National Toxicology Program

National Toxicology Program

Caffeine

http://ntp.niehs.nih.gov/go/TS-10036-G

CASRN: 58-08-2

Formula: C8-H10-N4-O2

Synonyms/Common Names:

  • 3,7-DIHYDRO-1,3,7-TRIMETHYL-1H-PURINE (9CI)

Known Uses:

Multipurpose food substance, flavor enhancer, stimulant, diuretic used in the treatment of shock, asthma and heart disease. Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Nomination Background

Short-Term Toxicity

  • 2 weeks (Dosed-Water)  (C02733)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 13 weeks (Dosed-Water)  (C02733)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0,188,3000, M: 0,94,1500 PPM/12 PER GROUP.

Special Studies

  • C. elegans  (S0873)  Completed 
    • C. elegans

Genetic Toxicology

  • Salmonella (NCI)  (N-9342)  Completed 
    • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. (1985) Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli. Environ. Mutagen. 7 (Suppl 5): 1-248.
    • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli Environ. Mutagen. Vol. 7 (Suppl 5) (1985) 1-248
    • Salmonella
    • Salmonella-NCI  Negative 
  • Salmonella  (075951)  Completed 
    • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. (1985) Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli. Environ. Mutagen. 7 (Suppl 5): 1-248.
    • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
    •  Negative 

Organ Systems Toxicity

  • 120 day minimum Continuous Breeding (Legacy) (Dosed-Water)  (RACB81078)  Completed 
    • NTIS Number: PB85-101202
      Citation: Final Report - Caffeine: Reproduction and Fertility Assessment in CD-1 Mice When Administered in the Drinking Water
    • Male and Female Mice: Swiss
    • Dose: 0,.012,.033,.1,.3,1.0%; 0,.012,.025,.05%; N/A; 0,.05%.
  • 120 day minimum Continuous Breeding (Legacy) (Dosed-Water)  (RACB84044)  Completed 
    • NTIS Number: PB85-205052
      Citation: Final Report - Caffeine: Reproduction and Fertility Assessment In CD-1 Mice When Administered in Drinking Water
    • Male and Female Mice: Swiss
    • Dose: 0,.01,.03,.1,.3,1.0%; 0,.012,.025,.05%; 0,.05%; N/A.
  • 14 weeks Continuous Breeding (Legacy) (Gavage)  (RACB94006)  Completed 
    • NTIS Number: PB96-211743
      Citation: Reproductive Toxicity of Caffeine (CAS #58-08-2) Administered by Gavage to Sprague-Dawley Rats
    • Male and Female Rats: Sprague Dawley
    • Dose: TASK 1: 0, 50, 100, 150, OR 200 MG/KG; TASK 2: 0, 12.5, 25, OR 50 MG/KG; TASK 3: 0 OR 50 MG/KG; TASK 4: 0, 12.5, 25, OR 50 MG/KG.
  • 11 GD Conventional Teratology (Intravenous)  (T0009A)  Completed 
    • Female Rats: Sprague Dawley
    • Dose: 37.5, 75, 112.5, 150 MG/KG/DAY.
  • 0 to 19 GD Conventional Teratology (Gavage)  (T0009D)  Completed 
    • Female Rats: Sprague Dawley
    • Dose: 40, 80 MG/KG/DAY.
The NTP is administratively located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.

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