National Toxicology Program

CASRN: 109-99-9

Formula: C4-H8-O

Synonyms/Common Names:

• 1,4-EPOXYBUTANE

Known Uses:

Reaction medium for grignard and metal hydride reactions. Packaging fabrication. Solvent for resins and plastics. Chemical intermediate. Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Short-Term Toxicity

• 14-Day (Gavage)  (C60560A)  Completed 
  • Rats: F344/N; Mice: B6C3F1
• 13-Week (Gavage)  (C60560A)  Completed 
  • Rats: F344/N; Mice: B6C3F1
  • Dose: R: 0,125,2000, M: 0,63,1000 MG/KG/10 PER GROUP.
• 13-WEEK (Inhalation)  (C60560B)  Completed 
  • Rats: F344/N; Mice: B6C3F1
  • Dose: R&M: 0,66,200,600,1800,5000 PPM.

Long-Term Carcinogenicity

• 2-YEAR (Inhalation)  (C60560C)  Report Complete 
  • TR-475 (NIH Number: 98-3965; NTIS Number: PB98-164544)  (Peer Review Approval 12/11/1996A )
    Toxicology and Carcinogenesis Studies of Tetrahydrofuran (CASRN 109-99-9) in F344/N Rats and B6C3F1 Mice (Inhalation Studies)
  • Rats: F344/N; Mice: B6C3F1
  • Carcinogenesis Results
  • Male Rats Some Evidence 
  • Female Rats No Evidence 
  • Male Mice No Evidence 
  • Female Mice Clear Evidence 
  • Dose: R&M: 0, 200, 600, OR 1800 PPM (50/SEX/SPECIES/GROUP).

Genetic Toxicology

• Chromosome Aberrations  (720076)  Completed 
  • Mice
  • Chromosome Aberation  Negative 
• In Vitro Cytogenetics (CA/SCE)  (378945)  Completed 
  • Citation: Galloway, S., Armstrong, M., Reuben, C., Colman, S., Brown, B., Cannon, C., Bloom, A., Nakamura, F., Ahmed, M., Duk, S., Rimpo, J., Margolin, B., Resnick, M., Anderson, B., and Zeiger, E. Chromosome aberration and sister chromatid exchanges in Chinese hamster ovary cells: Evaluations of 108 chemicals. Environ. Molec. Mutagen. Vol. 10 (Suppl 10) (1987) 1-176
  • Sister Chromatid Exchange Negative 
  • Chromosome Aberation Negative 
• Drosophila  (442740)  Completed 
  • Citation: Valencia, R., Mason, J.M., Woodruff, R.C., and Zimmering, S. Chemical mutagenesis testing in Drosophila: III. Results of 48 coded compounds tested for the National Toxicology Program. Environ. Mutagen. Vol. 7 (1985) 325-348
  • Reciprocal Translocation/Sex-Linked Recessive Lethal Negative 
• Micronucleus  (A34612)  Completed 
  • Citation: Witt, K.L., Knapton, A., Wehr, C.M., Hook, G.J., Mirsalis, J., Shelby, M.D., and MacGregor, J.T. Micronucleated Erythrocyte Frequency in Peripheral Blood of B6C3F1 Mice from Short-Term, Prechronic, and Chronic Studies of the NTP Carcinogenesis Bioassay Program. Environ. Molec. Mutagen. Vol. 36 (2000) 163-194
  • Mice: B6C3F1
  • Male Equivocal 
  • Female Negative 
• Salmonella  (551903)  Completed 
  • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
  •  Negative 
• Salmonella  (620162)  Completed 
  • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
  •  Negative 
• Sister Chromatid Exchanges  (720076)  Completed 
  • Mice
  • Sister Chromatid  Positive (Nonstandard Protocol) 

Organ Systems Toxicity

• 6 to 17 GD for mice Conventional Teratology (Inhalation)  (TER88047)  Completed 
  • NTIS Number: DE89001383
    Citation: Inhalation Developmental Toxicology Studies: Teratology Study of Tetrahydrofuran in Mice and Rats (August 1988)
  • Female Rats/Mice: Sprague Dawley/Swiss
  • Rats/Mice Maternal Positive 
  • Rats/Mice Fetal Positive 
  • Dose: 600, 1800, 5000 PPM.
• 6 to 19 days GD Conventional Teratology (Inhalation)  (TER88139)  Completed 
  • NTIS Number: DE89001383
    Citation: Inhalation Developmental Toxicology Studies: Teratology Study of Tetrahydrofuran in Mice and Rats (August 1988)
  • Female Rats: Sprague Dawley
  • Dose: 0, 600, 1800, 5000 PPM.