This page has links to current ICCVAM-recommended protocols for the listed methods, the ICCVAM Test Method Evaluation Reports in which the protocols were originally published, and pages on the ICCVAM website on which additional information about the evaluation of the method can be found.
Please Note: Many of the complete ICCVAM Test Method Evaluation Reports are large documents and may take a few minutes to download.
Select links to jump to test method area of interest:
Revised Up-and-Down Procedure for Acute Oral Toxicity Testing
In Vitro Cytotoxicity Test Methods for Estimating Starting Doses for Acute Oral Systemic Toxicity Tests
Protocols for the BG1Luc Estrogen Receptor Transactivation Assays
In Vitro Test Methods for Assessing Potential to Cause Chemically Induced Eye Injuries
Please note: Some of the protocols listed below are recommended only for nonregulatory, validation, or optimization studies. Such limitations are noted on the title pages of the relevant protocols.
In Vitro Test Methods Proposed for Assessing Pyrogenicity of Pharmaceuticals and Other Products
Protocols were published as Appendix C to ICCVAM Test Method Evaluation Report, NIH Document No. 10-7553, 2010
More information about the ICCVAM evaluation of in vitro pyrogen testing methods
Please note: Availability of the Mono Mac 6 cell line for commercial testing purposes may be limited. See the in vitro pyrogen test method evaluation page of the ICCVAM website for details.
Updated Protocol for the Murine Local Lymph Node Assay for Assessing Allergic Contact Dermatitis Hazard Potential
Nonradiolabeled Murine Local Lymph Node Assay Methods for Assessing Allergic Contact Dermatitis Hazard Potential