National Toxicology Program

National Toxicology Program
http://ntp.niehs.nih.gov/go/ts-m950118

1,2-Dibromo-2,4-dicyanobutane - M950118

CASRN: 35691-65-7

Formula: C6-H6-Br2-N2

Synonyms/Common Names:

  • 2-BROMO-2-(BROMOMETHYL)GLUTARONITRILE
  • 2-BROMO-2-(BROMOMETHYL)-PENTANEDINITRILE (9CI)
  • DIBROMODICYANOBUTANE
  • METACIDE 38
  • METHYLDIBROMOGLUTARONITRILE
  • TEKTAMER 38

Known Uses:

As a preservative in paints, emulsions, dispersed pigments, adhesives, joint cements, metalworking fluids, paper, inks, waxes, household products, and cosmetics. As a biocide protects water-based systems from bacteria, fungi, yeast and algae. (exec sum)

Nomination Background

Short-Term Toxicity

  • 2 weeks (Topical Application)  (C97003B)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0, 37, 75, 150, 300, OR 600 MG/KG (GIVEN IN TWO DOSES); M: 0, 75, 150, 300, 600, OR 1200 MG/KG; 5/SEX/SPECIES/DOSE.
  • 2 weeks (Gavage)  (C97003)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0, 37, 75, 150, 300, OR 600 MG/KG; M: 0, 75, 150, 300, 600, OR 1200 MG/KG; 5/SEX/SPECIES/DOSE.
  • 13 weeks (Topical Application)  (C97003C)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0, 0.2, 0.6, 2, 6, OR 18 MG/KG, 5X PER WEEK; 10/SEX/DOSE.

Long-Term Carcinogenicity

  • 2 years (Topical Application)  (C97003D)  Completed 
    • TR-555 (NIH Number: 10-5896; NTIS Number: PB2010-113180)  (Peer Review Approval 02/27/2008A )
      Toxicology and Carcinogenesis Studies of 1,2-Dibromo-2,4-dicyanobutane (CASRN 35691-65-7) in F344/N Rats and B6C3F1 Mice (Dermal Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats No Evidence 
      • Female Rats No Evidence 
      • Male Mice No Evidence 
      • Female Mice No Evidence 
    • Dose: R: 0, 2, 6, OR 18 MG/KG AND M: 0, 0.6, 2, OR 6 MG/KG; 50/SEX/SPECIES/D0SE.

Special Studies

  • Mechanisms  (S0783)  Completed 
    • Citation: Sauer JM, Smith RL, Bao J, Kattnig MJ, Kuester RK, Sipes IG. Metabolic and dispositional fate of 1,2-dibromo-2,4-dicyanobutane in the male fischer 344 Rat. Drug Metab Dispos. 1998 May;26(5):429-36.
    • Male Rats: F344/N
  • Mechanisms  (S0784)  Completed 
    • Citation: Bao J, Sauer JM, Smith RL, Kuester RK, Kattnig MJ, Sipes IG. Sulfhydryl-dependent biotransformation and macromolecular binding of 1,2-dibromo-2,4-dicyanobutane in blood. Drug Metab Dispos. 1998 Oct;26(10):1001-7.
    • Male Rats: F344/N
  • Metabolism (Intravenous)  (S0806)  Completed 
    • Citation: Bao J, Sauer JM, Smith RL, Kuester RK, Kattnig MJ, Sipes IG. Sulfhydryl-dependent biotransformation and macromolecular binding of 1,2-dibromo-2,4-dicyanobutane in blood. Drug Metab Dispos. 1998 Oct;26(10):1001-7.
    • Male Rats: F344/N
  • Metabolism (Gavage; Intravenous; Topical Application)  (S0807)  Completed 
    • Citation: Sauer JM, Smith RL, Bao J, Kattnig MJ, Kuester RK, Sipes IG. Metabolic and dispositional fate of 1,2-dibromo-2,4-dicyanobutane in the male fischer 344 Rat. Drug Metab Dispos. 1998 May;26(5):429-36.
    • Male Rats: F344/N

Genetic Toxicology

  • Micronucleus  (A65692)  Completed 
    • Mice: B6C3F1
    • Male Negative 
    • Female Negative 
  • Salmonella  (A11889)  Completed 
    •  Negative 
  • Salmonella  (A92777)  Completed 
    •  Negative 
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.