TDMS Study 88070-03 Pathology Tables
NTP Experiment-Test: 88070-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/11/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 10:10:25 CORE STUDY Facility: Battelle Northwest Chemical CAS #: 115-11-0007 Lock Date: 10/05/95 Cage Range: All Reasons For Removal: All Removal Date Range: All Treatment Groups: Include All a Number of animals examined microscopically at site and number of animals with lesion Page 1 NTP Experiment-Test: 88070-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/11/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 10:10:25 ____________________________________________________________________________________________________________________________________ FISCHER 344 RATS FEMALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ DISPOSITION SUMMARY Animals Initially In Study 50 50 50 50 Early Deaths Moribund Sacrifice 21 25 15 23 Natural Death 6 5 2 5 Accidently Killed 1 Survivors Terminal Sacrifice 23 19 33 22 Animals Examined Microscopically 50 50 50 50 ____________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM Esophagus (50) (50) (50) (50) Inflammation, Suppurative 1 (2%) Intestine Large, Colon (49) (50) (49) (49) Inflammation, Acute 1 (2%) Parasite Metazoan 5 (10%) 1 (2%) 1 (2%) Intestine Large, Rectum (47) (47) (47) (48) Parasite Metazoan 8 (17%) 5 (11%) 5 (11%) 3 (6%) Intestine Large, Cecum (48) (49) (48) (49) Inflammation, Acute 1 (2%) 1 (2%) Parasite Metazoan 5 (10%) 2 (4%) 4 (8%) 3 (6%) Intestine Small, Jejunum (47) (47) (48) (47) Inflammation, Acute 1 (2%) Necrosis 1 (2%) Intestine Small, Ileum (46) (47) (48) (47) Inflammation, Acute 1 (2%) Parasite Metazoan 1 (2%) Liver (50) (50) (50) (49) Angiectasis 1 (2%) 3 (6%) 4 (8%) 3 (6%) Basophilic Focus 36 (72%) 36 (72%) 43 (86%) 36 (73%) Clear Cell Focus 6 (12%) 9 (18%) 12 (24%) 5 (10%) Cytomegaly 1 (2%) Degeneration, Cystic 2 (4%) Degeneration, Fatty 12 (24%) 10 (20%) 12 (24%) 12 (24%) Eosinophilic Focus 6 (12%) 6 (12%) 5 (10%) 9 (18%) Hematopoietic Cell Proliferation 1 (2%) Hepatodiaphragmatic Nodule 4 (8%) 7 (14%) 5 (10%) 3 (6%) Inflammation, Granulomatous 1 (2%) Mitotic Alteration 1 (2%) Mixed Cell Focus 7 (14%) 11 (22%) 9 (18%) 8 (16%) Necrosis 2 (4%) 2 (4%) Regeneration 1 (2%) 1 (2%) 2 (4%) 3 (6%) Thrombosis 1 (2%) Bile Duct, Hyperplasia 9 (18%) 5 (10%) 7 (14%) 10 (20%) a Number of animals examined microscopically at site and number of animals with lesion Page 2 NTP Experiment-Test: 88070-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/11/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 10:10:25 ____________________________________________________________________________________________________________________________________ FISCHER 344 RATS FEMALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM - CONT Centrilobular, Necrosis 4 (8%) 4 (8%) 5 (10%) 9 (18%) Mesentery (8) (10) (12) (7) Thrombosis 1 (8%) Artery, Inflammation, Chronic Active 2 (29%) Fat, Hemorrhage 1 (13%) Fat, Necrosis 8 (100%) 10 (100%) 12 (100%) 6 (86%) Pancreas (50) (50) (49) (49) Atrophy 9 (18%) 11 (22%) 17 (35%) 10 (20%) Basophilic Focus 1 (2%) 4 (8%) 3 (6%) 2 (4%) Hyperplasia 1 (2%) 1 (2%) Metaplasia, Hepatocyte 1 (2%) Salivary Glands (50) (50) (50) (50) Atrophy 1 (2%) 1 (2%) Stomach, Forestomach (50) (50) (50) (50) Hyperplasia, Basal Cell 1 (2%) Inflammation, Acute 1 (2%) Mineralization 1 (2%) Necrosis 7 (14%) 7 (14%) 6 (12%) 5 (10%) Stomach, Glandular (50) (50) (50) (49) Mineralization 1 (2%) Necrosis 3 (6%) 2 (4%) 1 (2%) 1 (2%) Tongue (1) (1) Hyperplasia, Squamous 1 (100%) Tooth (1) Necrosis 1 (100%) ____________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM Heart (50) (50) (49) (50) Cardiomyopathy 30 (60%) 29 (58%) 37 (76%) 31 (62%) Atrium, Thrombosis 1 (2%) ____________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM Adrenal Cortex (50) (50) (49) (49) Atrophy 1 (2%) 1 (2%) Degeneration, Cystic 2 (4%) 2 (4%) 4 (8%) 2 (4%) Hyperplasia 15 (30%) 16 (32%) 24 (49%) 21 (43%) Hypertrophy 11 (22%) 8 (16%) 9 (18%) 9 (18%) Necrosis 2 (4%) 3 (6%) 1 (2%) Thrombosis 1 (2%) Vacuolization Cytoplasmic 1 (2%) Adrenal Medulla (50) (50) (49) (49) Hyperplasia 10 (20%) 5 (10%) 6 (12%) 10 (20%) Islets, Pancreatic (50) (50) (49) (49) a Number of animals examined microscopically at site and number of animals with lesion Page 3 NTP Experiment-Test: 88070-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/11/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 10:10:25 ____________________________________________________________________________________________________________________________________ FISCHER 344 RATS FEMALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - CONT Hyperplasia 1 (2%) Pituitary Gland (50) (49) (50) (49) Angiectasis 1 (2%) Pars Distalis, Angiectasis 1 (2%) 1 (2%) Pars Distalis, Cyst 1 (2%) Pars Distalis, Hyperplasia 7 (14%) 7 (14%) 11 (22%) 5 (10%) Thyroid Gland (50) (50) (49) (49) C-Cell, Hyperplasia 37 (74%) 34 (68%) 38 (78%) 35 (71%) Follicular Cell, Hyperplasia 2 (4%) ____________________________________________________________________________________________________________________________________ GENERAL BODY SYSTEM None ____________________________________________________________________________________________________________________________________ GENITAL SYSTEM Clitoral Gland (50) (49) (47) (48) Hyperplasia 1 (2%) 5 (11%) 1 (2%) Inflammation, Chronic Active 1 (2%) Ovary (50) (50) (50) (50) Cyst 9 (18%) 4 (8%) 4 (8%) 2 (4%) Inflammation, Granulomatous 1 (2%) Uterus (50) (50) (50) (50) Fibrosis 1 (2%) Inflammation, Chronic Active 1 (2%) Necrosis 1 (2%) Cervix, Hypertrophy 1 (2%) Vagina (1) (1) (1) Inflammation, Suppurative 1 (100%) ____________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM Bone Marrow (50) (50) (49) (49) Atrophy 1 (2%) 1 (2%) 2 (4%) Hyperplasia, Histiocytic 1 (2%) Hyperplasia, Reticulum Cell 1 (2%) Lymph Node (6) (3) (3) (2) Pancreatic, Ectasia 1 (33%) Lymph Node, Mandibular (44) (46) (47) (47) Infiltration Cellular, Plasma Cell 1 (2%) Infiltration Cellular, Polymorphonuclear 1 (2%) Lymph Node, Mesenteric (50) (50) (49) (49) Infiltration Cellular, Plasma Cell 1 (2%) Spleen (50) (50) (49) (50) a Number of animals examined microscopically at site and number of animals with lesion Page 4 NTP Experiment-Test: 88070-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/11/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 10:10:25 ____________________________________________________________________________________________________________________________________ FISCHER 344 RATS FEMALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM - CONT Accessory Spleen 1 (2%) Fibrosis 3 (6%) 6 (12%) 2 (4%) Hematopoietic Cell Proliferation 5 (10%) 2 (4%) 3 (6%) 2 (4%) Hemorrhage 1 (2%) 2 (4%) Inflammation, Granulomatous 1 (2%) Necrosis 1 (2%) ____________________________________________________________________________________________________________________________________ INTEGUMENTARY SYSTEM Mammary Gland (50) (50) (50) (50) Galactocele 2 (4%) 2 (4%) 1 (2%) 2 (4%) Hyperplasia, Atypical 1 (2%) Inflammation, Acute 1 (2%) Inflammation, Chronic 1 (2%) Skin (50) (50) (50) (50) Cyst Epithelial Inclusion 1 (2%) Hyperkeratosis 1 (2%) Inflammation, Acute 1 (2%) Inflammation, Chronic Active 1 (2%) 1 (2%) 2 (4%) ____________________________________________________________________________________________________________________________________ MUSCULOSKELETAL SYSTEM Bone (50) (50) (50) (50) Hyperostosis 9 (18%) 7 (14%) 11 (22%) 15 (30%) Skeletal Muscle (1) Artery, Inflammation, Chronic 1 (100%) ____________________________________________________________________________________________________________________________________ NERVOUS SYSTEM Brain (50) (50) (50) (50) Hemorrhage 1 (2%) Artery, Inflammation, Chronic 1 (2%) ____________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM Larynx (50) (50) (50) (50) Epiglottis, Metaplasia, Squamous 2 (4%) Lung (50) (50) (50) (50) Inflammation, Granulomatous 7 (14%) 5 (10%) 9 (18%) 11 (22%) Inflammation, Suppurative 1 (2%) Alveolar Epithelium, Hyperplasia 9 (18%) 9 (18%) 7 (14%) 6 (12%) Alveolus, Infiltration Cellular, Histiocyte 1 (2%) Bronchiole, Hyperplasia 1 (2%) a Number of animals examined microscopically at site and number of animals with lesion Page 5 NTP Experiment-Test: 88070-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/11/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 10:10:25 ____________________________________________________________________________________________________________________________________ FISCHER 344 RATS FEMALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM - CONT Nose (50) (50) (50) (49) Inflammation, Suppurative 7 (14%) 2 (4%) 8 (16%) 8 (16%) Thrombosis 2 (4%) 3 (6%) 2 (4%) 3 (6%) Lateral Wall, Metaplasia, Squamous 3 (6%) Olfactory Epithelium, Degeneration, Hyaline 44 (88%) 47 (94%) 48 (96%) 47 (96%) Respiratory Epithelium, Metaplasia, Squamous 1 (2%) ____________________________________________________________________________________________________________________________________ SPECIAL SENSES SYSTEM Eye (4) (4) (6) (4) Cataract 4 (100%) 3 (75%) 6 (100%) 4 (100%) Retina, Atrophy 3 (75%) 3 (75%) 6 (100%) 4 (100%) ____________________________________________________________________________________________________________________________________ URINARY SYSTEM Kidney (50) (50) (49) (49) Infarct 1 (2%) 2 (4%) Nephropathy 46 (92%) 46 (92%) 46 (94%) 46 (94%) Renal Tubule, Hyperplasia 1 (2%) Urinary Bladder (50) (49) (49) (49) Hemorrhage 1 (2%) a Number of animals examined microscopically at site and number of animals with lesion Page 6 NTP Experiment-Test: 88070-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/11/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 10:10:25 ____________________________________________________________________________________________________________________________________ FISCHER 344 RATS MALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ DISPOSITION SUMMARY Animals Initially In Study 50 50 50 50 Early Deaths Moribund Sacrifice 39 40 37 41 Natural Death 4 5 7 1 Survivors Terminal Sacrifice 7 5 6 8 Animals Examined Microscopically 50 50 50 50 ____________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM Esophagus (50) (50) (50) (50) Inflammation, Suppurative 1 (2%) 1 (2%) 2 (4%) Intestine Large, Colon (49) (48) (48) (49) Diverticulum 1 (2%) Mineralization 1 (2%) Parasite Metazoan 4 (8%) 1 (2%) 2 (4%) 2 (4%) Intestine Large, Rectum (47) (49) (49) (49) Necrosis 1 (2%) Parasite Metazoan 2 (4%) 3 (6%) 3 (6%) Intestine Large, Cecum (48) (47) (49) (49) Inflammation, Acute 1 (2%) Necrosis 2 (4%) 1 (2%) Parasite Metazoan 3 (6%) 2 (4%) 6 (12%) 4 (8%) Intestine Small, Duodenum (49) (49) (50) (49) Necrosis 2 (4%) 2 (4%) Liver (50) (50) (50) (50) Angiectasis 3 (6%) 1 (2%) 1 (2%) Basophilic Focus 17 (34%) 14 (28%) 14 (28%) 22 (44%) Clear Cell Focus 3 (6%) 1 (2%) 3 (6%) 3 (6%) Cyst 2 (4%) Degeneration, Cystic 13 (26%) 10 (20%) 14 (28%) 16 (32%) Degeneration, Fatty 14 (28%) 18 (36%) 18 (36%) 14 (28%) Eosinophilic Focus 6 (12%) 2 (4%) 5 (10%) 5 (10%) Hepatodiaphragmatic Nodule 4 (8%) 2 (4%) 1 (2%) 2 (4%) Inflammation, Granulomatous 1 (2%) Mineralization 1 (2%) 1 (2%) Mixed Cell Focus 3 (6%) 2 (4%) 4 (8%) Necrosis 2 (4%) 3 (6%) 1 (2%) Regeneration 2 (4%) Thrombosis 2 (4%) Vacuolization Cytoplasmic, Focal 1 (2%) Bile Duct, Hyperplasia 23 (46%) 27 (54%) 23 (46%) 29 (58%) Centrilobular, Necrosis 10 (20%) 9 (18%) 10 (20%) 15 (30%) Mesentery (15) (10) (8) (11) a Number of animals examined microscopically at site and number of animals with lesion Page 7 NTP Experiment-Test: 88070-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/11/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 10:10:25 ____________________________________________________________________________________________________________________________________ FISCHER 344 RATS MALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ ALIMENTARY SYSTEM - CONT Mineralization 1 (7%) Pigmentation 1 (7%) Artery, Inflammation, Chronic Active 1 (10%) 1 (9%) Artery, Mineralization 1 (7%) 1 (10%) 1 (9%) Fat, Hemorrhage 1 (10%) 1 (9%) Fat, Necrosis 14 (93%) 9 (90%) 8 (100%) 8 (73%) Pancreas (50) (50) (50) (50) Atrophy 22 (44%) 17 (34%) 26 (52%) 18 (36%) Basophilic Focus 1 (2%) 1 (2%) 1 (2%) 2 (4%) Hyperplasia 4 (8%) 3 (6%) 4 (8%) 3 (6%) Artery, Inflammation 1 (2%) Artery, Mineralization 1 (2%) 1 (2%) Salivary Glands (50) (50) (50) (50) Artery, Mineralization 1 (2%) Stomach, Forestomach (50) (50) (50) (50) Inflammation, Chronic 1 (2%) Mineralization 1 (2%) 1 (2%) 1 (2%) 1 (2%) Necrosis 17 (34%) 16 (32%) 19 (38%) 10 (20%) Perforation 1 (2%) Stomach, Glandular (50) (49) (50) (50) Mineralization 3 (6%) 3 (6%) 5 (10%) 4 (8%) Necrosis 8 (16%) 5 (10%) 11 (22%) 9 (18%) Tongue (1) (1) Necrosis 1 (100%) Tooth (2) (3) (1) (1) Developmental Malformation 2 (100%) 1 (33%) 1 (100%) Inflammation, Chronic Active 2 (67%) 1 (100%) Necrosis 1 (33%) ____________________________________________________________________________________________________________________________________ CARDIOVASCULAR SYSTEM Blood Vessel (2) (2) (2) (2) Aorta, Mineralization 2 (100%) 2 (100%) 2 (100%) 2 (100%) Heart (50) (50) (50) (50) Cardiomyopathy 39 (78%) 39 (78%) 41 (82%) 41 (82%) Artery, Mineralization 3 (6%) 4 (8%) 4 (8%) 5 (10%) Atrium, Thrombosis 1 (2%) 3 (6%) 6 (12%) ____________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM Adrenal Cortex (50) (49) (50) (50) Atrophy 1 (2%) Degeneration, Cystic 1 (2%) Hyperplasia 21 (42%) 23 (47%) 24 (48%) 23 (46%) Hypertrophy 7 (14%) 6 (12%) 7 (14%) 4 (8%) a Number of animals examined microscopically at site and number of animals with lesion Page 8 NTP Experiment-Test: 88070-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/11/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 10:10:25 ____________________________________________________________________________________________________________________________________ FISCHER 344 RATS MALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ ENDOCRINE SYSTEM - CONT Necrosis 1 (2%) 1 (2%) Vacuolization Cytoplasmic 1 (2%) 3 (6%) Adrenal Medulla (50) (49) (50) (50) Atrophy 1 (2%) Hyperplasia 14 (28%) 18 (37%) 28 (56%) 15 (30%) Bilateral, Hyperplasia 1 (2%) 1 (2%) 2 (4%) 3 (6%) Islets, Pancreatic (50) (49) (50) (50) Hyperplasia 4 (8%) 3 (6%) 3 (6%) Parathyroid Gland (49) (50) (49) (47) Hyperplasia 9 (18%) 11 (22%) 12 (24%) 10 (21%) Pituitary Gland (50) (49) (50) (49) Angiectasis 1 (2%) Pars Distalis, Hyperplasia 5 (10%) 5 (10%) 3 (6%) 6 (12%) Pars Distalis, Thrombosis 1 (2%) Thyroid Gland (48) (48) (48) (50) C-Cell, Hyperplasia 32 (67%) 36 (75%) 34 (71%) 26 (52%) Follicular Cell, Hyperplasia 2 (4%) 1 (2%) ____________________________________________________________________________________________________________________________________ GENERAL BODY SYSTEM None ____________________________________________________________________________________________________________________________________ GENITAL SYSTEM Epididymis (50) (50) (50) (50) Granuloma Sperm 1 (2%) Preputial Gland (50) (50) (49) (50) Hyperplasia 2 (4%) 1 (2%) 2 (4%) 1 (2%) Inflammation, Acute 1 (2%) Inflammation, Chronic Active 3 (6%) 3 (6%) 2 (4%) Prostate (50) (49) (50) (50) Hyperplasia 6 (12%) 6 (12%) 6 (12%) 7 (14%) Inflammation, Chronic Active 4 (8%) 4 (8%) 4 (8%) 6 (12%) Seminal Vesicle (50) (47) (50) (50) Inflammation, Chronic Active 1 (2%) 1 (2%) Mineralization 1 (2%) 1 (2%) 1 (2%) Testes (50) (50) (50) (50) Atrophy 7 (14%) 3 (6%) 5 (10%) Necrosis 3 (6%) 1 (2%) Artery, Inflammation, Chronic Active 4 (8%) 2 (4%) 1 (2%) 3 (6%) Artery, Mineralization 1 (2%) Interstitial Cell, Hyperplasia 4 (8%) 4 (8%) 4 (8%) 2 (4%) ____________________________________________________________________________________________________________________________________ a Number of animals examined microscopically at site and number of animals with lesion Page 9 NTP Experiment-Test: 88070-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/11/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 10:10:25 ____________________________________________________________________________________________________________________________________ FISCHER 344 RATS MALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ HEMATOPOIETIC SYSTEM Bone Marrow (50) (48) (50) (50) Necrosis 1 (2%) Lymph Node (4) (11) (10) (10) Hemorrhage 1 (25%) Iliac, Ectasia 1 (10%) Renal, Hemorrhage 1 (9%) Lymph Node, Mandibular (47) (47) (49) (49) Hemorrhage 1 (2%) Infiltration Cellular, Plasma Cell 1 (2%) 1 (2%) 1 (2%) Infiltration Cellular, Polymorphonuclear 1 (2%) Lymph Node, Mesenteric (50) (50) (49) (50) Ectasia 1 (2%) 1 (2%) Lymph Node, Mediastinal (46) (42) (44) (45) Hemorrhage 1 (2%) Spleen (50) (49) (50) (50) Accessory Spleen 1 (2%) 1 (2%) 1 (2%) Fibrosis 9 (18%) 6 (12%) 10 (20%) 11 (22%) Hematopoietic Cell Proliferation 2 (4%) 5 (10%) 4 (8%) 3 (6%) Hemorrhage 1 (2%) 1 (2%) 1 (2%) Necrosis 2 (4%) 1 (2%) ____________________________________________________________________________________________________________________________________ INTEGUMENTARY SYSTEM Mammary Gland (41) (41) (40) (44) Galactocele 2 (5%) 7 (17%) 1 (3%) 4 (9%) Hyperplasia, Atypical 1 (2%) Inflammation, Chronic 1 (2%) 1 (3%) 1 (2%) Skin (50) (50) (50) (50) Cyst Epithelial Inclusion 1 (2%) Hyperkeratosis 1 (2%) 1 (2%) 1 (2%) Inflammation, Chronic Active 1 (2%) 1 (2%) 5 (10%) ____________________________________________________________________________________________________________________________________ MUSCULOSKELETAL SYSTEM Bone (50) (50) (50) (50) Fibrous Osteodystrophy 9 (18%) 7 (14%) 9 (18%) 6 (12%) Hyperostosis 1 (2%) 1 (2%) Skeletal Muscle (1) (1) (1) Mineralization 1 (100%) ____________________________________________________________________________________________________________________________________ a Number of animals examined microscopically at site and number of animals with lesion Page 10 NTP Experiment-Test: 88070-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/11/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 10:10:25 ____________________________________________________________________________________________________________________________________ FISCHER 344 RATS MALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ NERVOUS SYSTEM Brain (50) (50) (50) (50) Angiectasis 1 (2%) Hemorrhage 1 (2%) ____________________________________________________________________________________________________________________________________ RESPIRATORY SYSTEM Larynx (50) (49) (50) (49) Necrosis 1 (2%) 1 (2%) Epiglottis, Metaplasia, Squamous 1 (2%) Lung (50) (50) (50) (50) Cyst 1 (2%) Hemorrhage 1 (2%) 1 (2%) 1 (2%) Inflammation, Granulomatous 9 (18%) 3 (6%) 1 (2%) 5 (10%) Inflammation, Suppurative 1 (2%) 1 (2%) Metaplasia, Squamous 1 (2%) Mineralization 2 (4%) 2 (4%) 1 (2%) 1 (2%) Alveolar Epithelium, Hyperplasia 9 (18%) 5 (10%) 7 (14%) 5 (10%) Alveolus, Infiltration Cellular, Histiocyte 1 (2%) Artery, Mediastinum, Mineralization 2 (4%) 2 (4%) 1 (2%) 1 (2%) Bronchiole, Hyperplasia 1 (2%) Mediastinum, Inflammation, Suppurative 1 (2%) Nose (49) (49) (50) (49) Inflammation, Suppurative 14 (29%) 11 (22%) 16 (32%) 9 (18%) Thrombosis 4 (8%) 7 (14%) 10 (20%) 6 (12%) Lateral Wall, Metaplasia, Squamous 1 (2%) Olfactory Epithelium, Atrophy 3 (6%) Olfactory Epithelium, Degeneration, Hyaline 43 (88%) 45 (92%) 46 (92%) 49 (100%) Olfactory Epithelium, Metaplasia 4 (8%) Respiratory Epithelium, Metaplasia, Squamous 1 (2%) Trachea (50) (50) (50) (50) Inflammation, Suppurative 2 (4%) ____________________________________________________________________________________________________________________________________ SPECIAL SENSES SYSTEM Eye (3) (4) Cataract 2 (67%) 3 (75%) Degeneration 1 (33%) Inflammation, Chronic Active 2 (50%) Retina, Atrophy 1 (33%) 3 (75%) ____________________________________________________________________________________________________________________________________ a Number of animals examined microscopically at site and number of animals with lesion Page 11 NTP Experiment-Test: 88070-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC ISOBUTENE Date: 06/11/97 Route: RESPIRATORY EXPOSURE WHOLE BODY Time: 10:10:25 ____________________________________________________________________________________________________________________________________ FISCHER 344 RATS MALE CONTROL 500 PPM 2000 PPM 8000 PPM ____________________________________________________________________________________________________________________________________ URINARY SYSTEM Kidney (50) (50) (50) (50) Cyst 3 (6%) 3 (6%) 1 (2%) Hydronephrosis 1 (2%) Hyperplasia, Oncocytic 1 (2%) Infarct 1 (2%) 1 (2%) Mineralization 4 (8%) 2 (4%) 3 (6%) 4 (8%) Nephropathy 50 (100%) 48 (96%) 50 (100%) 50 (100%) Papilla, Necrosis 1 (2%) Pelvis, Inflammation, Acute 1 (2%) 1 (2%) 2 (4%) 1 (2%) Renal Tubule, Hyperplasia 2 (4%) 4 (8%) 3 (6%) 4 (8%) Transitional Epithelium, Hyperplasia 1 (2%) 2 (4%) Urinary Bladder (50) (49) (50) (50) Hemorrhage 1 (2%) 1 (2%) Inflammation, Chronic Active 1 (2%) 3 (6%) 2 (4%) Inflammation, Suppurative 1 (2%) Necrosis 1 (2%) Transitional Epithelium, Hyperplasia 1 (2%) 1 (2%) 1 (2%) 3 (6%) a Number of animals examined microscopically at site and number of animals with lesion Page 12 ------------------------------------------------------------ ---------- END OF REPORT ---------- ------------------------------------------------------------