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ICCVAM Biennial Report 2018-2019

ICCVAM Biennial Report 2018-2019
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https://ntp.niehs.nih.gov/go/884555

Validation of Androgen Receptor Activity Assays

NICEATM collaborated with test method developer CertiChem, Inc. to validate an in vitro test method that uses MDA-Kb2 human breast cancer cells to measure androgen receptor agonist and antagonist activity. Specifically, NICEATM provided guidance on incorporating a cytotoxicity assay into the test method protocol. Testing of 67 coded reference chemicals in agonist and antagonist modes to characterize method reliability and relevance is finished, and a report summarizing these results was provided to CertiChem in December of 2018.

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