National Toxicology Program

National Toxicology Program

Presentations: Workshop on Alternatives to the HIST

Please note that we have made some minor formatting changes to some of the presentations to improve accessibility, but none of the content of the presentations has been changed.

Opening Session

Session 1: Alternatives to HIST: Methods and Evaluations
Reports on Alternative Methods to HIST and Results Using Pertussis Toxin-Spiked Vaccine Samples

Biochemical Assays: Carbohydrate-Binding Assay; Enzymatic/HPLC Assays

Biochemical and Cell-Based Assays: Carbohydrate-Binding Assay, Enzymatic/HPLC Assays, CHO Cell Clustering Assay

Genetic Assays: Differential Gene Expression in Monocyte-Derived Dendritic Cells

  • Using Pertussis Toxin-Sensitive Genes in Dendritic Cells to Evaluate the Safety of Acellular Pertussis Vaccines
    Stefan F.C. Vaessen, PhD, University of Applied Sciences Utrecht, The Netherlands

  • Summary Analysis of Reported Data Sets
    Lev Sirota, PhD, CBER, U.S. FDA

Session 2: The Path Forward: Gaps to Cross and Bridges to Build in the Road Toward the Adoption of Alternatives to HIST

  • Opening Remarks
    Richard Isbrucker, PhD, Health Canada

  • Plenary Presentation: Pertussis Toxin and the CHO Cell Response
    Erik L. Hewlett, MD, University of Virginia School of Medicine, USA

NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.