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NICEATM Reference Chemical Lists for Test Method Evaluations

Below are lists of reference chemicals that may be useful for development or validation of new test methods. Some lists were developed during studies on new test methods and approaches conducted by NICEATM; others have been recommended by ICCVAM for validation of alternative test methods. Lists are downloadable as both Excel spreadsheets and tab-delimited text files.

The sections below also include links to the ICCVAM Test Method Evaluation Reports or references in the scientific literature in which the lists were originally published. Links to pages on the NICEATM website that have additional information about the context under which the lists were developed are also available. Users of these lists are encouraged to consult these resources to help them decide whether any of these lists might be useful for a specific test method development activity.

These lists are also available through the NICEATM Integrated Chemical Environment resource. In the "Integrator" section, select reference lists within the "Select Chemicals" window, then choose the reference list of interest from the window that opens.

Endocrine Disruptors

Androgen Receptor Agonist and Antagonist Chemicals

Published as Table 2 in:
Kleinstreuer NC, et al. Development and validation of a computational model for androgen receptor activity. Chem Res Toxicol. 2016;30(4):946-964. doi: 10.1021/acs.chemrestox.6b00347.

Estrogen Receptor Agonist and Antagonist Chemicals

Published as Tables 2 and 3 in:
Browne P, et al. Screening chemicals for estrogen receptor bioactivity using a computational model. Environ Sci Technol. 2015 Jul 21;49(14):8804-8814. doi: 10.1021/acs.est.5b02641.

Published as Table 1 in:
OECD. Test no. 455: performance-based test guideline for stably transfected transactivation in vitro assays to detect estrogen receptor agonists and antagonists. In: OECD guidelines for the testing of chemicals. Section 4. Paris: OECD Publishing; 2016. doi: 10.1787/9789264265295-en.

Curated Database of Rodent Uterotrophic Bioactivity

NICEATM compiled data from the peer-reviewed literature for in vivo uterotrophic assays that met six minimum criteria specified by accepted test guidelines. These data have potential utility for:

  • Developing adverse outcome pathways or models of estrogenic activity.
  • Prioritizing chemicals for further testing.
  • Evaluating species-specific responses to chemicals.

NICEATM Guideline-like Uterotrophic Database
More information about the Uterotrophic Database

Skin Sensitization

Recommended Reference Substances for the Murine Local Lymph Node Assay (LLNA)

Published as Appendix F in:
Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM). Recommended performance standards: murine local lymph node assay. National Institute of Environmental Health Sciences; 2010. NIH Publication No.: 09-7357.

NICEATM LLNA Database

Data from analyses of the LLNA conducted on behalf of ICCVAM by NICEATM are available to interested stakeholders as a reference for developing and evaluating alternative approaches to testing and assessment that replace, reduce, or refine the use of animals for identification of potential skin sensitizers.

Ocular Corrosion and Irritation

Recommended Substances for Optimization or Validation of In Vitro Ocular Toxicity Test Methods

Published as Appendix H in:
Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM). ICCVAM test method evaluation report: in vitro ocular toxicity test methods for identifying severe irritants and corrosives. National Institute of Environmental Health Sciences; 2006 Nov. NIH Publication No.: 07-4517.

More information about ICCVAM evaluations of in vitro ocular toxicity test methods

Acute Systemic Toxicity

Recommended Reference Substances for Evaluation of In Vitro Basal Cytotoxicity Methods for Predicting the Starting Dose for Rodent Acute Oral Toxicity Tests

Published as Table 3-1 in:
Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM). ICCVAM test method evaluation report: in vitro cytotoxicity test methods for estimating starting doses for acute oral systemic toxicity tests. National Institute of Environmental Health Sciences; 2006 Nov. NIH Publication No.: 07-4519.

More information about the NICEATM validation study of in vitro cytotoxicity test methods

Skin Corrosion and Irritation

Recommended Chemicals for Validation of New In Vitro Skin Corrosivity Test Methods

Published in:
Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM). Recommended performance standards for in vitro test methods for skin corrosion. National Institute of Environmental Health Sciences; 2004 May. NIH Publication No.: 04-4510.

More about development of the performance standards