Alternative Methods for Acute Inhalation Toxicity
NICEATM and the PETA International Science Consortium (PISC) presented a webinar series on Alternative Approaches for Acute Inhalation Toxicity to Address Global Regulatory and Non-regulatory Data Requirements. Webinar presenters:
- Reviewed regulatory guidelines relevant to assessing inhalation toxicity hazard potential
- Described alternative approaches for identifying chemicals likely to cause acute systemic toxicity via inhalation
- Identified mechanisms of acute toxicity that may constitute key events in adverse outcome pathways for acute inhalation toxicity
Following the webinar series, experts from industry, government, academia, and nongovernmental organizations met at the National Institutes of Health in September 2016 to discuss their experiences with alternative approaches for acute inhalation toxicity testing and consider how to develop strategies that regulatory agencies will accept. A manuscript describing workshop outcomes and recommendations is in preparation. A follow-up meeting is planned to discuss progress, identify outstanding issues, and define action plans to continue progress towards implementation.
In Vitro Testing Strategies to Assess Inhalation Toxicity of Nanomaterials
NICEATM is collaborating with PISC, the U.S. Environmental Protection Agency (EPA), and industry scientists to support a workgroup focused on inhalation toxicity. In 2014, NICEATM published a request for information (79 FR 35176, June 19, 2014) on devices and/or technologies currently used for identifying potential inhalation hazards. Subsequently, the workgroup met in 2015 to define specifications for the development and evaluation of an in vitro system to assess inhalation toxicity specifically of multiwalled carbon nanotubes. The recommended system would include different lung cells co-cultured at an air-liquid interface and consider relevant human dosimetry and nanomaterial lifecycle transformations. PISC used these recommendations for a subsequent request for proposals to develop an appropriate testing system; funding was awarded in September 2015. Presentations from the meeting and a list of publications developed by meeting participants are available on the PISC website.