One of NTP’s new study efforts is the Polycyclic Aromatic Compounds Mixtures Assessment Program (PAC-MAP), which aims to increase our understanding of the toxicity of PACs and PAC mixtures. NTP presented the research concept for PAC-MAP to the NTP Board of Scientific Counselors in December 2012.
NTP will use a wide range of tests in cells and rodents to assess the toxicity of individual PACs and select PAC mixtures and to identify potential health hazards. See the table below for a list of the studies and their descriptions.
|High throughput screening||Assays to derive information about cellular and molecular targets and use for predicting potential biological effects|
|Cytotoxicity and gene expression in diverse cell lines||Cell-based assays to evaluate cytotoxicity and gene expression changes|
|Zebrafish developmental effects||Short-term study to evaluate developmental effects in a vertebrate model system|
|Rat immunotoxicity (28-day)||Assay to evaluate effects on the immune system|
|Rat toxicity and toxicogenomics (28-day)||Assay to evaluate general toxicity and genomic changes|
In addition, NTP will design experiments to assess whether the toxicity of a PAC mixture found in the environment can be predicted using information about the toxicity of individual PACs (component-based-mixture approach for risk assessment). NTP will also generate data on PAC mixtures that can be used to predict the toxicity of untested mixtures (whole-mixture approach for risk assessment).
Results from PAC-MAP will contribute to addressing some of the knowledge gaps identified in the 2011 NIEHS workshop titled “Advancing Research on Mixtures: New Perspectives and Approaches for Predicting Adverse Human Health Effects.”