National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/5074

TR 371 Mouse Pathology Tables


                                     INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)
                                                              TOLUENE


NTP Experiment-Test: 05701-03                                                                                     Report: PEIRPT03
Study Type: CHRONIC                                                                                               Date: 09/18/94
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14




       Facility:  International Research and Development Corp.

       Chemical CAS #:  108883

       Lock Date:  None

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All
































a  Number of animals examined microscopically at site and number of animals with lesion

                                                             Page   1

NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                        0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY                                                                                                                 
  Animals Initially In Study                          60           60           60           60                                     
  Scheduled Sacrifice                                 10           10           10           10                                     
  Early Deaths                                                                                                                      
    Dead                                              11            6           13            9                                     
    Moribund                                           8            8           11            3                                     
    Accident                                           1            3                         1                                     
    Natural Death                                                                2            2                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                30           33           23           32                                     
    Dead                                                                         1                                                  
  Missing                                                                                     3                                     
  Animals Examined Microscopically                    50           50           50           47                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Esophagus                                          (49)         (50)         (50)         (47)                                   
      Infiltration Cellular, Lymphocyte                             2 (4%)                                                          
      Inflammation, Acute                                                        1 (2%)                                             
   Gallbladder                                        (49)         (49)         (50)         (45)                                   
      Hyperplasia, Lymphoid                            2 (4%)                                                                       
      Infiltration Cellular, Lymphocyte               14 (29%)     18 (37%)     15 (30%)     16 (36%)                               
      Inflammation, Acute                              1 (2%)       1 (2%)       1 (2%)       1 (2%)                                
      Inflammation, Chronic                                                      1 (2%)                                             
      Inflammation, Chronic Active                                               1 (2%)       1 (2%)                                
   Intestine Large                                    (50)         (50)         (50)         (47)                                   
      Anorectal Junction, Inflammation, Acute          1 (2%)                                                                       
      Anorectal Junction, Inflammation, Chronic                                                                                     
          Active                                                                 1 (2%)                                             
      Anorectal Junction, Ulcer                                                               1 (2%)                                
      Anus, Erosion                                                                           1 (2%)                                
      Anus, Inflammation, Acute                                                  1 (2%)       1 (2%)                                
      Anus, Inflammation, Chronic Active                                                      1 (2%)                                
      Anus, Ulcer                                                                1 (2%)                                             
   Intestine Large, Cecum                             (49)         (50)         (50)         (47)                                   
      Inflammation, Acute                              1 (2%)                                                                       
   Intestine Large, Rectum                            (50)         (50)         (50)         (45)                                   
      Inflammation, Acute                              1 (2%)                    1 (2%)       1 (2%)                                
   Intestine Small, Duodenum                          (49)         (50)         (50)         (47)                                   
      Inflammation, Chronic Active                                               1 (2%)                                             
   Intestine Small, Ileum                             (49)         (50)         (50)         (47)                                   
      Amyloid Deposition                               1 (2%)       2 (4%)       2 (4%)       1 (2%)                                
   Intestine Small, Jejunum                           (49)         (50)         (50)         (47)                                   
      Diverticulum                                                                            1 (2%)                                
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    2                                                              
NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                        0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
      Necrosis                                                                                1 (2%)                                
   Liver                                              (49)         (50)         (50)         (47)                                   
      Basophilic Focus                                 1 (2%)       3 (6%)                                                          
      Bile Stasis                                                                             1 (2%)                                
      Congestion                                                                              1 (2%)                                
      Cyst                                                          1 (2%)                                                          
      Fatty Change                                     1 (2%)                    1 (2%)       3 (6%)                                
      Hematopoietic Cell Proliferation                23 (47%)     16 (32%)     30 (60%)     26 (55%)                               
      Hyperplasia                                                   1 (2%)                                                          
      Hyperplasia, Lymphoid                            2 (4%)       1 (2%)                                                          
      Infarct                                          1 (2%)                                                                       
      Infiltration Cellular, Lymphocyte               23 (47%)     16 (32%)     15 (30%)     21 (45%)                               
      Inflammation, Acute                              1 (2%)                                 1 (2%)                                
      Inflammation, Chronic                                         1 (2%)                                                          
      Inflammation, Chronic Active                                                            1 (2%)                                
      Necrosis                                         7 (14%)      4 (8%)       4 (8%)       4 (9%)                                
      Thrombosis                                       1 (2%)                                                                       
      Vacuolization Cytoplasmic                                                  1 (2%)       1 (2%)                                
      Hepatocyte, Hypertrophy                          1 (2%)                                                                       
      Median Lobe, Angiectasis                                      1 (2%)                                                          
      Serosa, Inflammation, Chronic                                              1 (2%)       1 (2%)                                
   Mesentery                                          (5)          (2)          (4)          (2)                                    
      Inflammation, Chronic                                                      1 (25%)                                            
      Fat, Necrosis                                    1 (20%)                   1 (25%)                                            
   Pancreas                                           (50)         (50)         (50)         (47)                                   
      Hyperplasia, Lymphoid                            4 (8%)       1 (2%)                                                          
      Infiltration Cellular, Lymphocyte               17 (34%)     28 (56%)     25 (50%)     26 (55%)                               
      Inflammation, Acute                                           1 (2%)       1 (2%)                                             
      Inflammation, Chronic Active                     1 (2%)                                 1 (2%)                                
      Acinus, Atrophy                                  3 (6%)       1 (2%)       2 (4%)       2 (4%)                                
      Duct, Cyst                                       1 (2%)                    1 (2%)                                             
   Salivary Glands                                    (50)         (50)         (50)         (46)                                   
      Atrophy                                                       1 (2%)                                                          
      Hyperplasia, Lymphoid                            4 (8%)                                                                       
      Infiltration Cellular, Lymphocyte               28 (56%)     37 (74%)     33 (66%)     32 (70%)                               
   Stomach                                            (50)         (50)         (50)         (47)                                   
      Hyperplasia, Focal, Squamous                                               1 (2%)                                             
   Stomach, Forestomach                               (50)         (49)         (50)         (47)                                   
      Angiectasis                                                                             1 (2%)                                
      Erosion                                          1 (2%)       1 (2%)       2 (4%)       2 (4%)                                
      Hyperkeratosis                                   2 (4%)                                                                       
      Hyperplasia, Focal, Squamous                     2 (4%)       3 (6%)       2 (4%)       2 (4%)                                
      Hyperplasia, Squamous                            2 (4%)       6 (12%)      4 (8%)       4 (9%)                                
      Infiltration Cellular, Lymphocyte                5 (10%)      6 (12%)      8 (16%)      5 (11%)                               
      Inflammation, Acute                              2 (4%)       2 (4%)       6 (12%)      7 (15%)                               
      Inflammation, Chronic                                                                   1 (2%)                                
      Inflammation, Chronic Active                                  4 (8%)       3 (6%)       3 (6%)                                
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    3                                                              
NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                        0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
      Ulcer                                            1 (2%)       1 (2%)       4 (8%)       4 (9%)                                
   Stomach, Glandular                                 (49)         (50)         (50)         (47)                                   
      Edema                                                                      1 (2%)                                             
      Erosion                                          2 (4%)       4 (8%)       4 (8%)       2 (4%)                                
      Hyperplasia, Lymphoid                            2 (4%)                                                                       
      Infiltration Cellular, Lymphocyte               15 (31%)     18 (36%)     22 (44%)     23 (49%)                               
      Inflammation, Acute                              4 (8%)       2 (4%)       2 (4%)       2 (4%)                                
      Inflammation, Chronic                                                      1 (2%)                                             
      Inflammation, Chronic Active                                               1 (2%)                                             
      Metaplasia, Squamous                                          1 (2%)                                                          
      Mineralization                                                1 (2%)       2 (4%)       2 (4%)                                
      Ulcer                                            2 (4%)                    2 (4%)                                             
      Mucosa, Dilatation                              10 (20%)      6 (12%)     10 (20%)     14 (30%)                               
   Tooth                                                           (1)                                                              
      Pulp, Inflammation, Acute                                     1 (100%)                                                        
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Blood Vessel                                       (50)         (50)         (50)         (47)                                   
      Aorta, Mineralization                                         1 (2%)                                                          
   Heart                                              (50)         (50)         (50)         (47)                                   
      Fibrosis, Focal                                               1 (2%)                                                          
      Infiltration Cellular, Lymphocyte               10 (20%)     13 (26%)     20 (40%)     18 (38%)                               
      Inflammation, Acute                                                        3 (6%)                                             
      Inflammation, Chronic                            2 (4%)       1 (2%)       4 (8%)       1 (2%)                                
      Inflammation, Chronic Active                     2 (4%)                                                                       
      Mineralization                                   1 (2%)       1 (2%)                    1 (2%)                                
      Atrium, Thrombosis                                                         1 (2%)                                             
      Coronary Artery, Inflammation, Acute                          1 (2%)                                                          
      Coronary Artery, Inflammation, Chronic                        1 (2%)       1 (2%)                                             
      Valve, Thrombosis                                1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Gland                                      (49)         (50)         (50)         (47)                                   
      Capsule, Inflammation, Acute                                               1 (2%)                                             
      Capsule, Spindle Cell, Hyperplasia              49 (100%)    50 (100%)    49 (98%)     45 (96%)                               
   Adrenal Gland, Cortex                              (49)         (50)         (50)         (47)                                   
      Congestion                                                                              1 (2%)                                
      Cyst                                             1 (2%)                                 1 (2%)                                
      Degeneration, Fatty                              1 (2%)       9 (18%)      2 (4%)       1 (2%)                                
      Hematopoietic Cell Proliferation                                                        1 (2%)                                
      Hyperplasia                                      1 (2%)                                                                       
      Hypertrophy                                      1 (2%)       2 (4%)       2 (4%)       4 (9%)                                
      Hypertrophy, Diffuse                                                                    1 (2%)                                
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    4                                                              
NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                        0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ENDOCRINE SYSTEM - CONT                                                                                                             
      Necrosis                                                                   1 (2%)                                             
      Pigmentation                                    35 (71%)     37 (74%)     45 (90%)     37 (79%)                               
      Thrombosis                                       1 (2%)                    1 (2%)                                             
      Spindle Cell, Hyperplasia                                                               1 (2%)                                
   Adrenal Gland, Medulla                             (49)         (50)         (49)         (47)                                   
      Hyperplasia                                      1 (2%)                    1 (2%)                                             
      Necrosis                                                                   1 (2%)                                             
   Islets, Pancreatic                                 (50)         (49)         (50)         (47)                                   
      Hyperplasia                                      1 (2%)       1 (2%)       1 (2%)                                             
   Pituitary Gland                                    (49)         (48)         (49)         (46)                                   
      Pars Distalis, Angiectasis                       2 (4%)                    2 (4%)                                             
      Pars Distalis, Cyst                                           3 (6%)       3 (6%)                                             
      Pars Distalis, Hemorrhage                                                  1 (2%)                                             
      Pars Distalis, Hyperplasia                      12 (24%)     18 (38%)     14 (29%)     15 (33%)                               
   Thyroid Gland                                      (50)         (50)         (50)         (47)                                   
      Hyperplasia, Lymphoid                            1 (2%)                                                                       
      Infiltration Cellular, Lymphocyte                5 (10%)      6 (12%)      8 (16%)      6 (13%)                               
      Inflammation, Acute                                           1 (2%)       1 (2%)       1 (2%)                                
      Inflammation, Chronic                                                      1 (2%)                                             
      Inflammation, Chronic Active                                  1 (2%)                    3 (6%)                                
      C-Cell, Hyperplasia                                                        1 (2%)                                             
      Follicle, Dilatation                             8 (16%)      5 (10%)      4 (8%)       5 (11%)                               
      Follicle, Hyperplasia                            9 (18%)     13 (26%)      7 (14%)     10 (21%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Clitoral Gland                                     (1)          (1)          (1)          (1)                                    
      Abscess                                          1 (100%)                                                                     
      Cyst                                                                       1 (100%)                                           
   Ovary                                              (50)         (49)         (50)         (47)                                   
      Abscess                                                                    2 (4%)                                             
      Hemorrhage                                                                 1 (2%)       2 (4%)                                
      Hyperplasia, Lymphoid                            2 (4%)                                                                       
      Hyperplasia, Adenomatous                                                                1 (2%)                                
      Infiltration Cellular, Lymphocyte                                          1 (2%)                                             
      Inflammation, Acute                                                        2 (4%)       4 (9%)                                
      Mineralization                                                                          1 (2%)                                
      Pigmentation, Hemosiderin, Cholesterol                                     1 (2%)                                             
      Follicle, Cyst                                                             1 (2%)                                             
      Follicle, Cyst Multilocular                                                1 (2%)                                             
      Periovarian Tissue, Cyst                        11 (22%)      8 (16%)      8 (16%)      7 (15%)                               
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    5                                                              
NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                        0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
GENITAL SYSTEM - CONT                                                                                                               
   Oviduct                                                                      (1)                                                 
      Inflammation, Acute                                                        1 (100%)                                           
   Uterus                                             (50)         (50)         (50)         (47)                                   
      Angiectasis                                      1 (2%)       2 (4%)                    1 (2%)                                
      Endometrium, Hyperplasia, Cystic                45 (90%)     43 (86%)     38 (76%)     38 (81%)                               
      Endometrium, Inflammation, Acute                 4 (8%)       1 (2%)       7 (14%)      6 (13%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (50)         (50)         (50)         (47)                                   
      Angiectasis                                      1 (2%)                                                                       
      Fibrosis                                        25 (50%)     32 (64%)     26 (52%)     21 (45%)                               
      Fibrous Osteodystrophy                                                                  4 (9%)                                
      Myeloid Cell, Hyperplasia                       33 (66%)     35 (70%)     38 (76%)     34 (72%)                               
   Lymph Node                                         (48)         (49)         (50)         (46)                                   
      Hemorrhage                                       1 (2%)                                                                       
      Hyperplasia, Lymphoid                            1 (2%)       2 (4%)       2 (4%)                                             
      Inflammation, Acute                              1 (2%)                                                                       
      Axillary, Hyperplasia, Lymphoid                                                         1 (2%)                                
      Bronchial, Hyperplasia, Lymphoid                                           1 (2%)                                             
      Iliac, Hyperplasia, Lymphoid                     3 (6%)                                 2 (4%)                                
      Iliac, Inflammation, Acute                                                              1 (2%)                                
      Mediastinal, Congestion                                                                 2 (4%)                                
      Mediastinal, Hyperplasia, Lymphoid               1 (2%)       1 (2%)       2 (4%)       5 (11%)                               
      Mediastinal, Inflammation, Acute                                           1 (2%)                                             
      Mesenteric, Angiectasis                          1 (2%)                                 1 (2%)                                
      Mesenteric, Congestion                                        5 (10%)      6 (12%)      7 (15%)                               
      Mesenteric, Hemorrhage                                                                  1 (2%)                                
      Mesenteric, Hyperplasia, Lymphoid               26 (54%)     17 (35%)     21 (42%)     24 (52%)                               
      Mesenteric, Inflammation, Acute                  1 (2%)       2 (4%)       7 (14%)      7 (15%)                               
      Renal, Congestion                                                          1 (2%)                                             
      Renal, Hyperplasia, Lymphoid                     2 (4%)                    1 (2%)       1 (2%)                                
      Renal, Inflammation, Acute                                                 1 (2%)       1 (2%)                                
   Lymph Node, Mandibular                             (47)         (48)         (50)         (43)                                   
      Congestion                                                                              1 (2%)                                
      Hyperplasia                                                   1 (2%)       1 (2%)                                             
      Hyperplasia, Glandular                                                     1 (2%)                                             
      Hyperplasia, Lymphoid                           31 (66%)     29 (60%)     32 (64%)     25 (58%)                               
      Inflammation, Acute                                           2 (4%)       2 (4%)       1 (2%)                                
      Pigmentation                                                  1 (2%)       1 (2%)                                             
   Spleen                                             (50)         (50)         (49)         (47)                                   
      Angiectasis                                                                             1 (2%)                                
      Hematopoietic Cell Proliferation                41 (82%)     39 (78%)     41 (84%)     45 (96%)                               
      Hyperplasia, Lymphoid                           16 (32%)     16 (32%)     13 (27%)     14 (30%)                               
      Infiltration Cellular                                                      1 (2%)                                             
      Inflammation, Chronic                            1 (2%)                                                                       
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    6                                                              
NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                        0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - CONT                                                                                                         
      Necrosis                                         1 (2%)                                                                       
      Pigmentation                                    37 (74%)     33 (66%)     34 (69%)     28 (60%)                               
      Capsule, Inflammation, Acute                                               1 (2%)                                             
   Thymus                                             (46)         (48)         (48)         (47)                                   
      Congestion                                                    1 (2%)                    1 (2%)                                
      Cyst                                             2 (4%)       1 (2%)       1 (2%)       2 (4%)                                
      Ectopic Thyroid                                                            1 (2%)                                             
      Edema                                                                      1 (2%)                                             
      Hyperplasia, Lymphoid                            7 (15%)      6 (13%)     10 (21%)     10 (21%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (49)         (50)         (48)         (46)                                   
      Ectasia                                         16 (33%)     18 (36%)     18 (38%)     11 (24%)                               
      Inflammation, Acute                                           1 (2%)                                                          
      Acinus, Ectasia                                                            1 (2%)                                             
   Skin                                               (50)         (50)         (50)         (47)                                   
      Alopecia                                         9 (18%)      9 (18%)      8 (16%)      2 (4%)                                
      Inflammation, Acute                              1 (2%)                                                                       
      Inflammation, Chronic                                                      1 (2%)                                             
      Ulcer                                            1 (2%)                                                                       
      Back, Ulcer                                                   1 (2%)                                                          
      Foot, Ulcer                                                                1 (2%)                                             
      Head, Ulcer                                      1 (2%)                    1 (2%)                                             
      Inguinal, Inflammation, Acute                    1 (2%)                                                                       
      Inguinal, Ulcer                                                            1 (2%)                                             
      Subcutaneous Tissue, Edema                                                 1 (2%)       1 (2%)                                
      Subcutaneous Tissue, Hemorrhage                  1 (2%)       1 (2%)                                                          
      Subcutaneous Tissue, Inflammation, Acute                                   1 (2%)       1 (2%)                                
      Ventral, Inflammation, Chronic                   1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (50)         (49)         (50)         (47)                                   
      Cranium, Fracture                                                                       1 (2%)                                
      Cranium, Inflammation, Chronic Active                                                   1 (2%)                                
      Femur, Fracture                                               1 (2%)       1 (2%)       1 (2%)                                
   Skeletal Muscle                                                 (1)          (2)                                                 
      Head, Inflammation, Acute                                                  2 (100%)                                           
____________________________________________________________________________________________________________________________________
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    7                                                              
NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                        0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (50)         (50)         (50)         (47)                                   
      Compression                                                                3 (6%)       1 (2%)                                
      Hemorrhage                                       5 (10%)                   4 (8%)       2 (4%)                                
      Infiltration Cellular, Lymphocyte                4 (8%)       3 (6%)       4 (8%)       3 (6%)                                
      Mineralization                                  35 (70%)     31 (62%)     30 (60%)     28 (60%)                               
      Hippocampus, Cyst                                                                       1 (2%)                                
      Meninges, Inflammation, Acute                                 1 (2%)                    1 (2%)                                
      Nerve, Inflammation, Acute                                                 1 (2%)                                             
      Ventricle, Dilatation                            1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (50)         (50)         (47)                                   
      Congestion                                      13 (26%)      2 (4%)       9 (18%)      8 (17%)                               
      Hemorrhage                                       5 (10%)      8 (16%)      7 (14%)      6 (13%)                               
      Hyperplasia, Lymphoid                            5 (10%)      1 (2%)                                                          
      Infiltration Cellular, Lymphocyte               30 (60%)     42 (84%)     40 (80%)     43 (91%)                               
      Mineralization                                   2 (4%)       7 (14%)                   1 (2%)                                
      Pigmentation, Cholesterol                        1 (2%)                                                                       
      Alveolar Epithelium, Hyperplasia                 2 (4%)       3 (6%)       3 (6%)       4 (9%)                                
      Alveolus, Infiltration Cellular, Histiocyte      2 (4%)       2 (4%)       3 (6%)       7 (15%)                               
      Interstitium, Inflammation, Acute                3 (6%)                    1 (2%)       1 (2%)                                
      Interstitium, Inflammation, Chronic                           1 (2%)       1 (2%)                                             
      Peribronchiolar, Inflammation, Acute             1 (2%)                    1 (2%)                                             
      Pleura, Inflammation, Chronic                                              1 (2%)       1 (2%)                                
      Pleura, Interstitium, Inflammation, Acute                                  1 (2%)                                             
   Nose                                               (50)         (50)         (50)         (47)                                   
      Lumen, Hemorrhage                               31 (62%)     28 (56%)     28 (56%)     25 (53%)                               
      Mucosa, Inflammation, Acute                      3 (6%)       1 (2%)       4 (8%)       6 (13%)                               
      Nasolacrimal Duct, Hemorrhage                    1 (2%)       1 (2%)       1 (2%)                                             
      Nasolacrimal Duct, Inflammation, Acute                        1 (2%)       5 (10%)      2 (4%)                                
      Olfactory Epithelium, Degeneration              48 (96%)     31 (62%)     41 (82%)     31 (66%)                               
      Olfactory Epithelium, Inflammation, Acute                     1 (2%)                    3 (6%)                                
      Olfactory Epithelium, Metaplasia                                                        3 (6%)                                
      Respiratory Epithelium, Degeneration            18 (36%)     19 (38%)     14 (28%)     12 (26%)                               
      Respiratory Epithelium, Inflammation, Acute                                             2 (4%)                                
      Turbinate, Inflammation, Acute                   1 (2%)       1 (2%)       1 (2%)                                             
      Vomeronasal Organ, Inflammation, Acute           1 (2%)                                                                       
   Trachea                                            (50)         (50)         (50)         (47)                                   
      Hemorrhage                                                                              1 (2%)                                
      Inflammation, Acute                                                        1 (2%)                                             
      Glands, Inflammation, Acute                                                             1 (2%)                                
      Lumen, Hemorrhage                                                                       1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    8                                                              
NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                        0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                             (1)                                                              
      Atrophy                                                       1 (100%)                                                        
   Harderian Gland                                                 (3)          (2)          (3)                                    
      Hyperplasia                                                                             1 (33%)                               
      Infiltration Cellular, Lymphocyte                             1 (33%)                                                         
      Inflammation, Acute                                                                     1 (33%)                               
      Inflammation, Chronic Active                                                            1 (33%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (50)         (50)         (50)         (47)                                   
      Abscess                                                       1 (2%)       1 (2%)                                             
      Congestion                                                                              1 (2%)                                
      Hemorrhage                                                                 1 (2%)                                             
      Hyperplasia, Lymphoid                            6 (12%)      1 (2%)                                                          
      Infarct                                          1 (2%)                                 1 (2%)                                
      Infiltration Cellular, Lymphocyte               31 (62%)     42 (84%)     36 (72%)     38 (81%)                               
      Inflammation                                                               1 (2%)                                             
      Inflammation, Acute                                                                     1 (2%)                                
      Inflammation, Chronic                                                      1 (2%)                                             
      Metaplasia, Osseous                              4 (8%)       1 (2%)       3 (6%)       4 (9%)                                
      Mineralization                                                1 (2%)                                                          
      Regeneration                                                                            1 (2%)                                
      Capsule, Inflammation, Chronic                                             1 (2%)                                             
      Pelvis, Dilatation                               2 (4%)       1 (2%)       1 (2%)                                             
      Pelvis, Inflammation, Acute                                                2 (4%)                                             
      Renal Tubule, Casts Protein                      6 (12%)     11 (22%)      5 (10%)     12 (26%)                               
      Renal Tubule, Dilatation                        24 (48%)     16 (32%)     30 (60%)     24 (51%)                               
      Renal Tubule, Necrosis                           5 (10%)      7 (14%)      9 (18%)      6 (13%)                               
      Renal Tubule, Pigmentation                                    1 (2%)       1 (2%)       1 (2%)                                
      Renal Tubule, Regeneration                      14 (28%)     13 (26%)     23 (46%)     15 (32%)                               
   Ureter                                                          (1)          (1)                                                 
      Dilatation                                                    1 (100%)     1 (100%)                                           
      Infiltration Cellular, Lymphocyte                             1 (100%)                                                        
   Urinary Bladder                                    (50)         (50)         (50)         (47)                                   
      Angiectasis                                      1 (2%)                                 1 (2%)                                
      Ectasia                                                                    2 (4%)                                             
      Hyperplasia, Lymphoid                            5 (10%)      1 (2%)                                                          
      Infiltration Cellular, Lymphocyte               31 (62%)     39 (78%)     34 (68%)     35 (74%)                               
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    9                                                              
NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                          0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY                                                                                                                 
  Animals Initially In Study                          60           60           60           60                                     
  Early Deaths                                                                                                                      
    Moribund                                          19           18           23           17                                     
    Dead                                              23           19           16           21                                     
    Accident                                           1                         2            2                                     
    Natural Death                                                   1            3            1                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                17           21           16           19                                     
    Dead                                                            1                                                               
  Animals Examined Microscopically                    60           60           60           60                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Esophagus                                          (59)         (60)         (60)         (57)                                   
      Inflammation, Chronic                                         1 (2%)                                                          
   Gallbladder                                        (59)         (54)         (57)         (59)                                   
      Cyst                                             1 (2%)                                                                       
      Infiltration Cellular, Lymphocyte                6 (10%)      8 (15%)      8 (14%)      7 (12%)                               
      Inflammation, Acute                              1 (2%)       1 (2%)       2 (4%)                                             
      Inflammation, Chronic                            1 (2%)                                                                       
      Inflammation, Chronic Active                                  1 (2%)                                                          
   Intestine Large                                    (60)         (60)         (60)         (60)                                   
      Anorectal Junction, Erosion                                   1 (2%)       1 (2%)                                             
      Anorectal Junction, Inflammation, Acute                                                 1 (2%)                                
      Anus, Erosion                                                              2 (3%)       1 (2%)                                
      Anus, Inflammation, Acute                                                  1 (2%)                                             
      Anus, Inflammation, Chronic Active                            1 (2%)       1 (2%)                                             
      Anus, Ulcer                                                   2 (3%)       2 (3%)       3 (5%)                                
   Intestine Large, Cecum                             (60)         (60)         (60)         (59)                                   
      Parasite Metazoan                                                          2 (3%)       3 (5%)                                
   Intestine Large, Colon                             (60)         (60)         (60)         (59)                                   
      Parasite Metazoan                                1 (2%)       1 (2%)       1 (2%)       2 (3%)                                
   Intestine Large, Rectum                            (59)         (59)         (60)         (58)                                   
      Hemorrhage                                                    1 (2%)                                                          
      Inflammation, Acute                                                        1 (2%)                                             
      Inflammation, Chronic                                                      1 (2%)                                             
      Ulcer                                            4 (7%)       2 (3%)       1 (2%)                                             
      Anorectal Junction, Ulcer                                                               1 (2%)                                
   Intestine Small, Ileum                             (60)         (60)         (60)         (60)                                   
      Amyloid Deposition                               1 (2%)       1 (2%)       1 (2%)       1 (2%)                                
   Intestine Small, Jejunum                           (60)         (60)         (60)         (60)                                   
      Hyperplasia, Lymphoid                            1 (2%)                                                                       
   Liver                                              (60)         (60)         (60)         (59)                                   
      Basophilic Focus                                              2 (3%)                    3 (5%)                                
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   10                                                              
NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                          0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
      Bile Stasis                                      1 (2%)                                                                       
      Cyst                                                                       1 (2%)       1 (2%)                                
      Focal Cellular Change                            1 (2%)                                                                       
      Hematopoietic Cell Proliferation                21 (35%)     13 (22%)     17 (28%)     17 (29%)                               
      Hyperplasia                                                   1 (2%)                    2 (3%)                                
      Hypertrophy                                                                             1 (2%)                                
      Infarct                                          1 (2%)       1 (2%)       1 (2%)       2 (3%)                                
      Infiltration Cellular, Lymphocyte                2 (3%)       7 (12%)     10 (17%)      3 (5%)                                
      Inflammation, Acute                              3 (5%)       1 (2%)       2 (3%)       2 (3%)                                
      Inflammation, Chronic                            3 (5%)       1 (2%)       1 (2%)                                             
      Necrosis                                         5 (8%)       6 (10%)      4 (7%)       9 (15%)                               
      Bile Duct, Hyperplasia                                                                  1 (2%)                                
      Caudate Lobe, Infarct                                         1 (2%)                                                          
      Centrilobular, Fatty Change                      1 (2%)                                                                       
      Periportal, Inflammation, Chronic                2 (3%)                                                                       
      Serosa, Inflammation, Chronic                                                           1 (2%)                                
   Mesentery                                          (2)                       (2)          (1)                                    
      Artery, Inflammation, Chronic                                              1 (50%)                                            
      Artery, Inflammation, Chronic Active             1 (50%)                                                                      
   Pancreas                                           (60)         (60)         (60)         (59)                                   
      Cyst                                                                                    1 (2%)                                
      Fibrosis                                                                   1 (2%)                                             
      Infiltration Cellular, Lymphocyte               11 (18%)     14 (23%)     18 (30%)     14 (24%)                               
      Inflammation, Acute                                           1 (2%)                                                          
      Inflammation, Chronic                                                      1 (2%)                                             
      Inflammation, Chronic Active                                  1 (2%)                                                          
      Acinus, Atrophy                                  1 (2%)       3 (5%)       1 (2%)                                             
      Acinus, Hyperplasia                              1 (2%)                    1 (2%)                                             
   Salivary Glands                                    (60)         (60)         (60)         (59)                                   
      Hemorrhage                                       1 (2%)                                                                       
      Hyperplasia, Glandular                           1 (2%)                                                                       
      Infiltration Cellular                                         1 (2%)                                                          
      Infiltration Cellular, Lymphocyte               30 (50%)     36 (60%)     34 (57%)     30 (51%)                               
      Mineralization                                                                          1 (2%)                                
   Stomach, Forestomach                               (60)         (59)         (60)         (60)                                   
      Erosion                                                                                 1 (2%)                                
      Hyperkeratosis                                   1 (2%)                    1 (2%)                                             
      Hyperplasia, Focal, Squamous                                                            1 (2%)                                
      Hyperplasia, Squamous                                         7 (12%)      1 (2%)                                             
      Infiltration Cellular, Lymphocyte                1 (2%)       3 (5%)       1 (2%)       1 (2%)                                
      Inflammation, Acute                              4 (7%)       2 (3%)                    2 (3%)                                
      Inflammation, Chronic                                                      1 (2%)                                             
      Inflammation, Chronic Active                     2 (3%)                    1 (2%)                                             
      Ulcer                                            1 (2%)       1 (2%)       1 (2%)                                             
   Stomach, Glandular                                 (60)         (60)         (60)         (60)                                   
      Edema                                                                                   2 (3%)                                
      Erosion                                          4 (7%)       6 (10%)      6 (10%)      4 (7%)                                
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   11                                                              
NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                          0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
      Infiltration Cellular, Lymphocyte               14 (23%)     14 (23%)     17 (28%)     15 (25%)                               
      Inflammation, Acute                              2 (3%)       2 (3%)                    3 (5%)                                
      Inflammation, Chronic Active                     1 (2%)       1 (2%)                                                          
      Mucosa, Dilatation                               2 (3%)       6 (10%)      5 (8%)      13 (22%)                               
      Mucosa, Hyperplasia                              1 (2%)                                                                       
      Submucosa, Edema                                                           1 (2%)                                             
   Tooth                                              (2)          (8)          (2)          (1)                                    
      Peridontal Tissue, Inflammation, Chronic                                                                                      
          Active                                                    2 (25%)                                                         
      Pulp, Inflammation, Acute                        2 (100%)     2 (25%)      2 (100%)                                           
      Pulp, Inflammation, Chronic                                   1 (13%)                                                         
      Pulp, Inflammation, Chronic Active                            3 (38%)                                                         
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Blood Vessel                                       (59)         (60)         (60)         (60)                                   
      Aorta, Inflammation, Chronic                                                            1 (2%)                                
      Aorta, Mineralization                            1 (2%)                                 1 (2%)                                
   Heart                                              (60)         (60)         (60)         (59)                                   
      Atrophy                                                                    1 (2%)                                             
      Infiltration Cellular, Lymphocyte                9 (15%)     13 (22%)      9 (15%)      8 (14%)                               
      Inflammation, Acute                                           1 (2%)       1 (2%)       2 (3%)                                
      Inflammation, Chronic                            5 (8%)       4 (7%)       1 (2%)       2 (3%)                                
      Inflammation, Chronic Active                                               1 (2%)       2 (3%)                                
      Mineralization                                                             1 (2%)                                             
      Atrium, Thrombosis                                                                      1 (2%)                                
      Ventricle Right, Thrombosis                                                1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Gland                                      (60)         (60)         (59)         (60)                                   
      Capsule, Hyperplasia                                                       1 (2%)                                             
      Capsule, Spindle Cell, Hyperplasia              59 (98%)     57 (95%)     53 (90%)     55 (92%)                               
   Adrenal Gland, Cortex                              (60)         (60)         (59)         (59)                                   
      Hyperplasia                                                   3 (5%)       2 (3%)                                             
      Hypertrophy                                      4 (7%)       3 (5%)       3 (5%)       3 (5%)                                
      Pigmentation                                     8 (13%)      7 (12%)     10 (17%)     16 (27%)                               
   Adrenal Gland, Medulla                             (60)         (58)         (58)         (59)                                   
      Hyperplasia                                      1 (2%)       1 (2%)                                                          
   Islets, Pancreatic                                 (60)         (60)         (60)         (58)                                   
      Hyperplasia                                                   1 (2%)       1 (2%)                                             
      Necrosis                                                                   1 (2%)                                             
   Pituitary Gland                                    (59)         (58)         (58)         (56)                                   
      Pars Distalis, Cyst                              1 (2%)       1 (2%)                                                          
      Pars Distalis, Hyperplasia                       2 (3%)       1 (2%)                                                          
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   12                                                              
NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                          0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ENDOCRINE SYSTEM - CONT                                                                                                             
      Pars Intermedia, Hyperplasia                                                            1 (2%)                                
   Thyroid Gland                                      (60)         (60)         (60)         (59)                                   
      Infiltration Cellular, Lymphocyte                8 (13%)      5 (8%)       2 (3%)       3 (5%)                                
      Inflammation, Acute                                                                     1 (2%)                                
      C-Cell, Hyperplasia                                           1 (2%)                                                          
      Follicle, Cyst                                   2 (3%)                    1 (2%)                                             
      Follicle, Dilatation                             3 (5%)       2 (3%)                    3 (5%)                                
      Follicle, Hyperplasia                            4 (7%)       5 (8%)       2 (3%)       3 (5%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Epididymis                                         (60)         (60)         (60)         (60)                                   
      Granuloma Sperm                                  3 (5%)                                 1 (2%)                                
      Infiltration Cellular, Lymphocyte                9 (15%)      3 (5%)       8 (13%)      4 (7%)                                
      Inflammation, Acute                                           1 (2%)       2 (3%)       3 (5%)                                
      Inflammation, Chronic                                                      2 (3%)       1 (2%)                                
      Inflammation, Chronic Active                                  1 (2%)       1 (2%)       4 (7%)                                
   Penis                                              (17)         (21)         (17)         (21)                                   
      Abscess                                                       3 (14%)      1 (6%)                                             
      Concretion                                       1 (6%)                                                                       
      Inflammation                                     1 (6%)       1 (5%)                    1 (5%)                                
      Inflammation, Acute                             16 (94%)     10 (48%)     13 (76%)     12 (57%)                               
      Inflammation, Chronic                                         2 (10%)                   1 (5%)                                
      Inflammation, Chronic Active                                  2 (10%)                   1 (5%)                                
      Necrosis                                                      2 (10%)      4 (24%)      4 (19%)                               
   Preputial Gland                                    (12)         (9)          (10)         (11)                                   
      Abscess                                          4 (33%)      2 (22%)      3 (30%)      3 (27%)                               
      Cyst                                             3 (25%)      2 (22%)      2 (20%)      2 (18%)                               
      Infiltration Cellular, Lymphocyte                             2 (22%)      1 (10%)                                            
      Inflammation, Acute                              2 (17%)      1 (11%)                   2 (18%)                               
      Inflammation, Chronic                            3 (25%)      1 (11%)      2 (20%)                                            
      Inflammation, Chronic Active                     1 (8%)       1 (11%)      3 (30%)      2 (18%)                               
   Prostate                                           (60)         (60)         (59)         (59)                                   
      Infiltration Cellular, Lymphocyte                8 (13%)      6 (10%)     16 (27%)     14 (24%)                               
      Inflammation, Acute                             14 (23%)     13 (22%)      9 (15%)     12 (20%)                               
      Inflammation, Chronic                            2 (3%)       2 (3%)                                                          
      Inflammation, Chronic Active                     2 (3%)                    3 (5%)       1 (2%)                                
   Seminal Vesicle                                    (4)          (4)          (4)          (6)                                    
      Dilatation                                                    1 (25%)      2 (50%)      1 (17%)                               
      Inflammation, Chronic                            1 (25%)      1 (25%)                                                         
      Inflammation, Chronic Active                                                            1 (17%)                               
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   13                                                              
NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                          0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
GENITAL SYSTEM - CONT                                                                                                               
   Testes                                             (60)         (60)         (60)         (60)                                   
      Atrophy                                          9 (15%)     12 (20%)      3 (5%)       3 (5%)                                
      Granuloma Sperm                                  2 (3%)                                                                       
      Hemorrhage                                       1 (2%)                                                                       
      Infiltration Cellular, Lymphocyte                1 (2%)                                                                       
      Inflammation, Acute                                                        1 (2%)       2 (3%)                                
      Mineralization                                                1 (2%)                                                          
      Interstitial Cell, Hyperplasia                   1 (2%)       1 (2%)       2 (3%)                                             
      Tunic, Inflammation, Chronic Active                                        1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (60)         (60)         (60)         (59)                                   
      Fibrosis                                                                   1 (2%)       1 (2%)                                
      Inflammation, Acute                                           1 (2%)                                                          
      Myeloid Cell, Hyperplasia                       51 (85%)     48 (80%)     56 (93%)     44 (75%)                               
   Lymph Node                                         (60)         (60)         (59)         (59)                                   
      Congestion                                       2 (3%)                    1 (2%)                                             
      Hyperplasia, Lymphoid                            4 (7%)       3 (5%)       2 (3%)       1 (2%)                                
      Inflammation, Acute                                           1 (2%)       1 (2%)       1 (2%)                                
      Iliac, Hyperplasia, Lymphoid                    10 (17%)      4 (7%)       5 (8%)       4 (7%)                                
      Iliac, Hyperplasia, Reticulum Cell                            1 (2%)                                                          
      Iliac, Inflammation, Acute                                    1 (2%)       1 (2%)                                             
      Inguinal, Hyperplasia, Lymphoid                  2 (3%)                    3 (5%)       1 (2%)                                
      Lumbar, Hyperplasia, Lymphoid                                              2 (3%)                                             
      Mediastinal, Hyperplasia, Lymphoid               4 (7%)       1 (2%)       6 (10%)      5 (8%)                                
      Mesenteric, Autolysis                                                                   1 (2%)                                
      Mesenteric, Congestion                          20 (33%)     14 (23%)     12 (20%)     11 (19%)                               
      Mesenteric, Hematopoietic Cell Proliferation                                            1 (2%)                                
      Mesenteric, Hemorrhage                           1 (2%)       2 (3%)                                                          
      Mesenteric, Hyperplasia, Lymphoid               32 (53%)     24 (40%)     35 (59%)     27 (46%)                               
      Mesenteric, Inflammation, Acute                  4 (7%)       5 (8%)       3 (5%)      10 (17%)                               
      Renal, Congestion                                1 (2%)                                                                       
      Renal, Hyperplasia, Lymphoid                     2 (3%)                    2 (3%)       2 (3%)                                
   Lymph Node, Mandibular                             (53)         (52)         (54)         (51)                                   
      Autolysis                                                                               1 (2%)                                
      Congestion                                                                 1 (2%)                                             
      Hyperplasia, Lymphoid                           38 (72%)     40 (77%)     38 (70%)     32 (63%)                               
      Inflammation, Acute                              1 (2%)                                                                       
      Necrosis                                         1 (2%)                                                                       
      Pigmentation                                                               1 (2%)                                             
   Spleen                                             (60)         (60)         (60)         (59)                                   
      Angiectasis                                      1 (2%)                    2 (3%)                                             
      Congestion                                                    1 (2%)                                                          
      Hematopoietic Cell Proliferation                54 (90%)     50 (83%)     56 (93%)     47 (80%)                               
      Hyperplasia, Lymphoid                           10 (17%)      7 (12%)      6 (10%)      5 (8%)                                
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   14                                                              
NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                          0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - CONT                                                                                                         
      Hyperplasia, Reticulum Cell                                   1 (2%)                                                          
      Necrosis                                                                                2 (3%)                                
      Pigmentation                                     4 (7%)       9 (15%)     11 (18%)     18 (31%)                               
      Capsule, Inflammation, Chronic Active                                      1 (2%)                                             
   Thymus                                             (53)         (59)         (58)         (54)                                   
      Congestion                                                    1 (2%)                                                          
      Cyst                                             3 (6%)                    2 (3%)       1 (2%)                                
      Ectopic Parathyroid Gland                        1 (2%)                                                                       
      Hyperplasia, Lymphoid                            2 (4%)       2 (3%)       4 (7%)       2 (4%)                                
      Inflammation, Chronic Active                                  1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Skin                                               (60)         (60)         (60)         (58)                                   
      Alopecia                                         4 (7%)       5 (8%)       3 (5%)       5 (9%)                                
      Inflammation, Acute                              1 (2%)       1 (2%)                                                          
      Inflammation, Chronic                            2 (3%)       1 (2%)       1 (2%)                                             
      Ulcer                                            3 (5%)       1 (2%)       2 (3%)       4 (7%)                                
      Abdominal, Abscess                                            1 (2%)                                                          
      Abdominal, Thoracic, Alopecia                                 1 (2%)                                                          
      Foot, Ulcer                                                   1 (2%)       2 (3%)       1 (2%)                                
      Head, Abscess                                                 1 (2%)                    1 (2%)                                
      Head, Inflammation, Acute                        2 (3%)       1 (2%)                                                          
      Head, Inflammation, Chronic                      3 (5%)                    1 (2%)                                             
      Head, Ulcer                                                                             1 (2%)                                
      Inguinal, Abscess                                1 (2%)                                                                       
      Inguinal, Inflammation, Acute                                              1 (2%)                                             
      Inguinal, Ulcer                                  1 (2%)       4 (7%)       2 (3%)                                             
      Neck, Abscess                                                              1 (2%)                                             
      Neck, Alopecia                                                                          1 (2%)                                
      Prepuce, Abscess                                 2 (3%)       2 (3%)                                                          
      Prepuce, Inflammation, Acute                                                            1 (2%)                                
      Prepuce, Inflammation, Chronic Active                         1 (2%)                    1 (2%)                                
      Prepuce, Ulcer                                   9 (15%)     14 (23%)      8 (13%)     10 (17%)                               
      Scrotal, Abscess                                 5 (8%)                    1 (2%)       1 (2%)                                
      Scrotal, Inflammation, Acute                                  1 (2%)                                                          
      Scrotal, Inflammation, Chronic Active                                      1 (2%)                                             
      Scrotal, Ulcer                                  16 (27%)      9 (15%)     16 (27%)      8 (14%)                               
      Subcutaneous Tissue, Edema                                    1 (2%)                    1 (2%)                                
      Subcutaneous Tissue, Inflammation, Acute         2 (3%)       1 (2%)                                                          
      Subcutaneous Tissue, Inflammation, Chronic       1 (2%)                                                                       
      Subcutaneous Tissue, Head, Abscess               2 (3%)       1 (2%)       1 (2%)       1 (2%)                                
      Subcutaneous Tissue, Head, Granuloma                          1 (2%)                                                          
      Subcutaneous Tissue, Head, Inflammation,                                                                                      
           Acute                                       1 (2%)                                                                       
      Subcutaneous Tissue, Head, Inflammation,                                                                                      
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   15                                                              
NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                          0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
INTEGUMENTARY SYSTEM - CONT                                                                                                         
           Chronic Active                                           1 (2%)                                                          
      Tail, Inflammation, Acute                        1 (2%)                                                                       
      Tail, Inflammation, Chronic                                                1 (2%)                                             
      Tail, Necrosis                                   1 (2%)                                                                       
      Tail, Ulcer                                                   1 (2%)       1 (2%)                                             
      Thoracic, Alopecia                               1 (2%)                                                                       
      Ventral, Alopecia                                2 (3%)       2 (3%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (60)         (60)         (60)         (60)                                   
      Cranium, Inflammation, Chronic Active                         1 (2%)                    1 (2%)                                
      Femur, Hyperostosis                              1 (2%)                                 1 (2%)                                
      Tibia, Fracture                                                                         1 (2%)                                
   Skeletal Muscle                                                 (2)          (6)          (3)                                    
      Abscess                                                                    1 (17%)                                            
      Inflammation, Acute                                                                     1 (33%)                               
      Inflammation, Chronic                                                                   1 (33%)                               
      Inflammation, Chronic Active                                                            1 (33%)                               
      Head, Abscess                                                              4 (67%)                                            
      Head, Inflammation, Acute                                     1 (50%)                                                         
      Head, Inflammation, Chronic Active                                         1 (17%)                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (60)         (60)         (60)         (60)                                   
      Abscess                                                                                 1 (2%)                                
      Compression                                                                1 (2%)                                             
      Hemorrhage                                       2 (3%)       3 (5%)       2 (3%)       1 (2%)                                
      Infiltration Cellular, Lymphocyte                1 (2%)                                                                       
      Mineralization                                  40 (67%)     35 (58%)     41 (68%)     35 (58%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (60)         (60)         (60)         (60)                                   
      Abscess                                                                                 1 (2%)                                
      Congestion                                      11 (18%)     12 (20%)      5 (8%)       8 (13%)                               
      Granuloma                                                                  2 (3%)                                             
      Hemorrhage                                       6 (10%)     11 (18%)     16 (27%)      3 (5%)                                
      Infiltration Cellular, Lymphocyte               50 (83%)     54 (90%)     55 (92%)     52 (87%)                               
      Inflammation, Acute                                                        1 (2%)                                             
      Mineralization                                   2 (3%)                                                                       
      Alveolar Epithelium, Hyperplasia                 1 (2%)                    2 (3%)       1 (2%)                                
      Alveolus, Infiltration Cellular, Histiocyte      3 (5%)       1 (2%)       2 (3%)       3 (5%)                                
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   16                                                              
NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                          0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
RESPIRATORY SYSTEM - CONT                                                                                                           
      Arteriole, Inflammation, Acute                                             1 (2%)                                             
      Artery, Inflammation, Acute                                                1 (2%)                                             
      Interstitium, Inflammation, Acute                5 (8%)       1 (2%)       3 (5%)       4 (7%)                                
      Interstitium, Inflammation, Chronic              1 (2%)                                                                       
      Peribronchiolar, Inflammation, Acute             1 (2%)                                                                       
      Peribronchiolar, Inflammation, Chronic           2 (3%)       1 (2%)                                                          
      Peribronchiolar, Inflammation, Chronic Active                                           1 (2%)                                
      Perivascular, Inflammation, Acute                             1 (2%)                                                          
   Nose                                               (59)         (59)         (60)         (59)                                   
      Lumen, Hemorrhage                               36 (61%)     25 (42%)     40 (67%)     29 (49%)                               
      Mucosa, Inflammation, Acute                      1 (2%)       3 (5%)       1 (2%)       6 (10%)                               
      Nasolacrimal Duct, Hemorrhage                    2 (3%)       1 (2%)                                                          
      Nasolacrimal Duct, Inflammation, Acute           1 (2%)       4 (7%)       5 (8%)                                             
      Olfactory Epithelium, Degeneration              41 (69%)     11 (19%)     27 (45%)     30 (51%)                               
      Olfactory Epithelium, Metaplasia                                                        1 (2%)                                
      Respiratory Epithelium, Degeneration             7 (12%)      1 (2%)       8 (13%)      4 (7%)                                
      Respiratory Epithelium, Inflammation, Acute                   1 (2%)                                                          
      Septum, Inflammation, Acute                                                1 (2%)                                             
      Septum, Inflammation, Chronic Active                          1 (2%)                                                          
      Sinus, Inflammation, Acute                                                              1 (2%)                                
      Turbinate, Congestion                                                      1 (2%)       2 (3%)                                
      Turbinate, Inflammation, Acute                   1 (2%)                                                                       
      Vomeronasal Organ, Congestion                                 1 (2%)                                                          
      Vomeronasal Organ, Inflammation, Acute                        1 (2%)       2 (3%)                                             
   Trachea                                            (60)         (60)         (60)         (59)                                   
      Inflammation, Chronic Active                                               1 (2%)                                             
      Glands, Inflammation, Acute                                   3 (5%)                                                          
      Mucosa, Erosion                                  1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Harderian Gland                                                 (3)          (1)          (3)                                    
      Cyst                                                                                    1 (33%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (60)         (60)         (60)         (59)                                   
      Abscess                                                       1 (2%)       1 (2%)       1 (2%)                                
      Congestion                                       1 (2%)                                                                       
      Cyst                                             1 (2%)                                                                       
      Hemorrhage                                                                 1 (2%)                                             
      Hydronephrosis                                                1 (2%)                                                          
      Infiltration Cellular, Lymphocyte               53 (88%)     52 (87%)     52 (87%)     48 (81%)                               
      Inflammation, Acute                              4 (7%)       3 (5%)       3 (5%)       3 (5%)                                
      Inflammation, Chronic                            1 (2%)                                                                       
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   17                                                              
NTP Experiment-Test: 05701-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                              TOLUENE                                          Date: 09/18/94  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 14:49:14  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                          0 PPM        120 PPM      600 PPM      1200 PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
URINARY SYSTEM - CONT                                                                                                               
      Inflammation, Chronic Active                                  1 (2%)       1 (2%)       1 (2%)                                
      Metaplasia, Osseous                                                        1 (2%)                                             
      Capsule, Inflammation, Chronic                                                          1 (2%)                                
      Cortex, Cyst                                     2 (3%)       2 (3%)                    2 (3%)                                
      Pelvis, Calculus Micro Observation Only                                                 1 (2%)                                
      Pelvis, Dilatation                              14 (23%)     10 (17%)      9 (15%)      4 (7%)                                
      Pelvis, Hemorrhage                                            1 (2%)                    1 (2%)                                
      Pelvis, Inflammation, Acute                      7 (12%)      3 (5%)       5 (8%)       9 (15%)                               
      Pelvis, Inflammation, Chronic                    1 (2%)                                                                       
      Pelvis, Inflammation, Chronic Active             1 (2%)                                 1 (2%)                                
      Renal Tubule, Casts Protein                      4 (7%)       6 (10%)      4 (7%)       2 (3%)                                
      Renal Tubule, Cyst                               2 (3%)                    1 (2%)                                             
      Renal Tubule, Dilatation                        13 (22%)     17 (28%)     15 (25%)     17 (29%)                               
      Renal Tubule, Hyperplasia                        1 (2%)       3 (5%)       1 (2%)                                             
      Renal Tubule, Mineralization                     2 (3%)       6 (10%)      1 (2%)                                             
      Renal Tubule, Necrosis                           6 (10%)     12 (20%)      9 (15%)     12 (20%)                               
      Renal Tubule, Regeneration                      36 (60%)     30 (50%)     29 (48%)     21 (36%)                               
   Ureter                                             (8)          (1)          (3)                                                 
      Dilatation                                       3 (38%)      1 (100%)     3 (100%)                                           
      Inflammation, Acute                              1 (13%)                                                                      
   Urethra                                            (5)          (6)          (1)          (3)                                    
      Calculus Micro Observation Only                  3 (60%)      4 (67%)                   1 (33%)                               
      Inflammation, Acute                              1 (20%)      1 (17%)      1 (100%)     1 (33%)                               
      Inflammation, Chronic                            1 (20%)                                                                      
      Bulbourethral Gland, Inflammation, Acute                      1 (17%)                                                         
   Urinary Bladder                                    (60)         (60)         (60)         (59)                                   
      Angiectasis                                      2 (3%)       1 (2%)                                                          
      Calculus Gross Observation                       1 (2%)       1 (2%)                    2 (3%)                                
      Calculus Micro Observation Only                  1 (2%)                                 2 (3%)                                
      Congestion                                                    1 (2%)                                                          
      Ectasia                                         19 (32%)     10 (17%)     15 (25%)     13 (22%)                               
      Hemorrhage                                       1 (2%)                                                                       
      Infiltration Cellular, Lymphocyte               27 (45%)     25 (42%)     36 (60%)     31 (53%)                               
      Inflammation, Acute                             10 (17%)      8 (13%)      7 (12%)     11 (19%)                               
      Inflammation, Chronic                            1 (2%)       3 (5%)                                                          
      Inflammation, Chronic Active                     6 (10%)                   1 (2%)       4 (7%)                                
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   18                                                              
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NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.