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TR 383 Mouse Pathology Tables

NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: Mason                                          1-AMINO-2,4-DIBROMOANTHRAQUINONE                           DATE: 05/19/95       
EXPERIMENT: 05061 TEST: 02                                                                                     TIME: 17:13:40       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                   PAGE:       1        
CONT: N01ES9561903                              ROUTE: DOSED FEED                                              NTP C#: 55458        
PATHOLOGIST: R. FLEISCHMAN                                                                                     CAS: 81-49-2         
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               REASONS FOR REMOVAL:    ALL                                                                                          
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
               REMOVAL DATE RANGE:     ALL                                                                                          
                                                                                                                                    
               TREATMENT GROUPS:       INCLUDE ALL                                                                                  
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: Mason                                          1-AMINO-2,4-DIBROMOANTHRAQUINONE                           DATE: 05/19/95       
EXPERIMENT: 05061 TEST: 02                                                                                     TIME: 17:13:40       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                        
CONT: N01ES9561903                              ROUTE: DOSED FEED                                              NTP C#: 55458        
PATHOLOGIST: R. FLEISCHMAN                                                                                     CAS: 81-49-2         


                                                    MICE(B6C3F1)
                                              ----------------------------
             FOR ALL DOSES THE TUMOR RATES IN THE FOLLOWING TISSUES/ORGANS ARE BASED ON NUMBER OF TISSUES EXAMINED.
             IN OTHER TISSUES/ORGANS, RATES ARE BASED ON NUMBER OF ANIMALS NECROPSIED.
                                                                    
                                                    ----------------
                                                    Adrenal Cortex
                                                    Adrenal Medulla
                                                    Bone Marrow
                                                    Brain
                                                    Clitoral Gland
                                                    Clitoral/Preputial Gland
                                                    Epididymis
                                                    Gallbladder
                                                    Heart
                                                    Islets, Pancreatic
                                                    Kidney
                                                    Liver
                                                    Lung
                                                    Nose
                                                    Ovary
                                                    Pancreas
                                                    Parathyroid Gland
                                                    Pituitary Gland
                                                    Preputial Gland
                                                    Prostate
                                                    Salivary Glands
                                                    Spleen
                                                    Testes
                                                    Thymus
                                                    Thyroid Gland
                                                    Urinary Bladder
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: Mason                                          1-AMINO-2,4-DIBROMOANTHRAQUINONE                           DATE: 05/19/95       
EXPERIMENT: 05061 TEST: 02                                                                                     TIME: 17:13:40       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                        
CONT: N01ES9561903                              ROUTE: DOSED FEED                                              NTP C#: 55458        
PATHOLOGIST: R. FLEISCHMAN                                                                                     CAS: 81-49-2         


                                     SUMMARY OF STATISTICALLY SIGNIFICANT (P<=.05) RESULTS
                                IN THE ANALYSIS OF THE STUDY OF 1-AMINO-2,4-DIBROMOANTHRAQUINONE
              ====================================================================================================
                                                           MALE MICE
                                                          -------------
                  ORGAN                               MORPHOLOGY
                 -------                             ------------
              Liver                         Hepatoblastoma
                                            Hepatocellular Adenoma
                                            Hepatocellular Carcinoma
                                            Hepatocellular Carcinoma or Hepatoblastoma
                                            Hepatocellular Carcinoma or Hepatocellular Adenoma
                                            Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma
              Lung                          Alveolar/Bronchiolar Adenoma
                                            Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma
              Skin                          Fibroma, Fibrosarcoma, Sarcoma, Myxoma, Myxosarcoma, or Fibrous Histiocytoma
                                            Fibrosarcoma
                                            Fibrosarcoma, Sarcoma, Myxosarcoma, or Fibrous Histiocytoma
              Stomach, Forestomach          Squamous Cell Carcinoma
                                            Squamous Cell Carcinoma or Papilloma Squamous
                                            Squamous Cell Papilloma
              All Organs                    Benign Tumors
                                            Malignant Tumors
                                            Malignant and Benign Tumors
              ====================================================================================================
                                                           FEMALE MICE
                                                          -------------
                  ORGAN                               MORPHOLOGY
                 -------                             ------------
              Liver                         Hepatocellular Adenoma
                                            Hepatocellular Carcinoma
                                            Hepatocellular Carcinoma or Hepatoblastoma
                                            Hepatocellular Carcinoma or Hepatocellular Adenoma
                                            Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma
              Lung                          Alveolar/Bronchiolar Adenoma
                                            Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma
              Pituitary Gland: Pars Distalis or Unspecified Site
                                            Adenoma
              Stomach, Forestomach          Squamous Cell Carcinoma
                                            Squamous Cell Carcinoma or Papilloma Squamous
                                            Squamous Cell Papilloma
              Uterus                        Sarcoma Stromal or Polyp Stromal
              All Organs                    Hemangioma
                                            Hemangiosarcoma or Hemangioma
                                            Benign Tumors
                                            Malignant Tumors
                                            Malignant and Benign Tumors
              ====================================================================================================
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE   1
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|Adrenal Cortex                                                                                                                   |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     2/60 (3%)       1/60 (2%)       0/60 (0%)      |     0/60 (0%)       0/59 (0%)       0/58 (0%)      |
|ADJUSTED (b)           |      5.0%            4.5%            0.0%          |      0.0%            0.0%            0.0%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     2/40 (5%)       1/22 (5%)       0/23 (0%)      |     0/39 (0%)       0/34 (0%)       0/32 (0%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         729 (T)         ---           |      ---             ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.265N        P=0.703N        P=0.367N       |       (e)             (e)             (e)          |
|INCIDENTAL TUMOR       |     P=0.265N        P=0.703N        P=0.367N       |       (e)             (e)             (e)          |
|LOGISTIC REGRESSION    |     P=0.265N        P=0.703N        P=0.367N       |       (e)             (e)             (e)          |
|COCHRAN-ARMITAGE       |     P=0.142N                                       |       (e)                                          |
|FISHER EXACT           |                     P=0.500N        P=0.248N       |                       (e)             (e)          |
|=================================================================================================================================|
|                                                                                                                                 |
|Clitoral/Preputial Gland                                                                                                         |
|     Squamous Cell Carcinoma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     2/21 (10%)      0/16 (0%)       0/13 (0%)      |     0/0 (0%)        0/0 (0%)        0/1 (0%)       |
|ADJUSTED (b)           |      9.9%            0.0%            0.0%          |      0.0%            0.0%            0.0%          |
|INTERIM SACRIFICE 1 (c)|     1/5 (20%)       0/0             0/1 (0%)       |     0/0             0/0             0/0            |
|TERMINAL (d)           |     1/12 (8%)       0/5 (0%)        0/6 (0%)       |     0/0             0/0             0/0            |
|FIRST INCIDENCE (DAYS) |      458 (I)         ---             ---           |      ---             ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.306N        P=0.674N        P=0.500N       |       (e)             (e)             (e)          |
|INCIDENTAL TUMOR       |     P=0.306N        P=0.674N        P=0.500N       |       (e)             (e)             (e)          |
|LOGISTIC REGRESSION    |     P=0.145N        P=0.272N        P=0.393N       |       (e)             (e)             (e)          |
|COCHRAN-ARMITAGE       |     P=0.146N                                       |       (e)                                          |
|FISHER EXACT           |                     P=0.315N        P=0.374N       |                       (e)             (e)          |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE   2
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|Harderian Gland                                                                                                                  |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     2/60 (3%)       2/60 (3%)       1/60 (2%)      |     1/60 (2%)       2/60 (3%)       2/60 (3%)      |
|ADJUSTED (b)           |      5.0%            6.7%            4.3%          |      2.6%            5.9%            6.1%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     2/40 (5%)       0/22 (0%)       1/23 (4%)      |     1/39 (3%)       2/34 (6%)       2/33 (6%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         583             729 (T)       |      729 (T)         729 (T)         729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.566N        P=0.514         P=0.689N       |     P=0.332         P=0.452         P=0.442        |
|INCIDENTAL TUMOR       |     P=0.389N        P=0.571N        P=0.689N       |     P=0.332         P=0.452         P=0.442        |
|LOGISTIC REGRESSION    |     P=0.468N        P=0.637         P=0.689N       |     P=0.332         P=0.452         P=0.442        |
|COCHRAN-ARMITAGE       |     P=0.391N                                       |     P=0.391                                        |
|FISHER EXACT           |                     P=0.691N        P=0.500N       |                     P=0.500         P=0.500        |
|=================================================================================================================================|
|                                                                                                                                 |
|Harderian Gland                                                                                                                  |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     2/60 (3%)       2/60 (3%)       1/60 (2%)      |     1/60 (2%)       3/60 (5%)       2/60 (3%)      |
|ADJUSTED (b)           |      5.0%            6.7%            4.3%          |      2.6%            7.5%            6.1%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     2/40 (5%)       0/22 (0%)       1/23 (4%)      |     1/39 (3%)       2/34 (6%)       2/33 (6%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         583             729 (T)       |      729 (T)         372             729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.566N        P=0.514         P=0.689N       |     P=0.347         P=0.271         P=0.442        |
|INCIDENTAL TUMOR       |     P=0.389N        P=0.571N        P=0.689N       |     P=0.375         P=0.356         P=0.442        |
|LOGISTIC REGRESSION    |     P=0.468N        P=0.637         P=0.689N       |     P=0.398         P=0.308         P=0.442        |
|COCHRAN-ARMITAGE       |     P=0.391N                                       |     P=0.400                                        |
|FISHER EXACT           |                     P=0.691N        P=0.500N       |                     P=0.309         P=0.500        |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE   3
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|Intestine Small: Ileum                                                                                                           |
|     Carcinoma                                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/60 (0%)       1/60 (2%)       2/60 (3%)      |     0/60 (0%)       0/60 (0%)       0/60 (0%)      |
|ADJUSTED (b)           |      0.0%            4.5%            7.1%          |      0.0%            0.0%            0.0%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     0/40 (0%)       1/22 (5%)       0/23 (0%)      |     0/39 (0%)       0/34 (0%)       0/33 (0%)      |
|FIRST INCIDENCE (DAYS) |      ---             729 (T)         665           |      ---             ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.085         P=0.381         P=0.155        |       (e)             (e)             (e)          |
|INCIDENTAL TUMOR       |     P=0.191         P=0.381         P=0.478        |       (e)             (e)             (e)          |
|LOGISTIC REGRESSION    |     P=0.106         P=0.381         P=0.198        |       (e)             (e)             (e)          |
|COCHRAN-ARMITAGE       |     P=0.142                                        |       (e)                                          |
|FISHER EXACT           |                     P=0.500         P=0.248        |                       (e)             (e)          |
|=================================================================================================================================|
|                                                                                                                                 |
|Intestine Small: Site Unspecified                                                                                                |
|     Carcinoma                                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/60 (0%)       1/60 (2%)       2/60 (3%)      |     0/60 (0%)       0/60 (0%)       0/60 (0%)      |
|ADJUSTED (b)           |      0.0%            4.5%            7.1%          |      0.0%            0.0%            0.0%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     0/40 (0%)       1/22 (5%)       0/23 (0%)      |     0/39 (0%)       0/34 (0%)       0/33 (0%)      |
|FIRST INCIDENCE (DAYS) |      ---             729 (T)         665           |      ---             ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.085         P=0.381         P=0.155        |       (e)             (e)             (e)          |
|INCIDENTAL TUMOR       |     P=0.191         P=0.381         P=0.478        |       (e)             (e)             (e)          |
|LOGISTIC REGRESSION    |     P=0.106         P=0.381         P=0.198        |       (e)             (e)             (e)          |
|COCHRAN-ARMITAGE       |     P=0.142                                        |       (e)                                          |
|FISHER EXACT           |                     P=0.500         P=0.248        |                       (e)             (e)          |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE   4
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|Islets, Pancreatic                                                                                                               |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/51 (0%)       0/50 (0%)       0/49 (0%)      |     2/49 (4%)       1/50 (2%)       0/48 (0%)      |
|ADJUSTED (b)           |      0.0%            0.0%            0.0%          |      4.7%            2.9%            0.0%          |
|INTERIM SACRIFICE 1 (c)|     0/1 (0%)        0/0             0/1 (0%)       |     0/0             0/0             0/0            |
|TERMINAL (d)           |     0/40 (0%)       0/22 (0%)       0/23 (0%)      |     1/38 (3%)       1/34 (3%)       0/33 (0%)      |
|FIRST INCIDENCE (DAYS) |      ---             ---             ---           |      610             729 (T)         ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |       (e)             (e)             (e)          |     P=0.165N        P=0.533N        P=0.263N       |
|INCIDENTAL TUMOR       |       (e)             (e)             (e)          |     P=0.122N        P=0.572N        P=0.179N       |
|LOGISTIC REGRESSION    |       (e)             (e)             (e)          |     P=0.144N        P=0.492N        P=0.247N       |
|COCHRAN-ARMITAGE       |       (e)                                          |     P=0.144N                                       |
|FISHER EXACT           |                       (e)             (e)          |                     P=0.492N        P=0.253N       |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hemangiosarcoma                                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     3/60 (5%)       2/60 (3%)       1/60 (2%)      |     0/60 (0%)       0/60 (0%)       0/60 (0%)      |
|ADJUSTED (b)           |      7.5%            6.2%            2.2%          |      0.0%            0.0%            0.0%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     3/40 (8%)       1/22 (5%)       0/23 (0%)      |     0/39 (0%)       0/34 (0%)       0/33 (0%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         414             484           |      ---             ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.366N        P=0.655         P=0.461N       |       (e)             (e)             (e)          |
|INCIDENTAL TUMOR       |     P=0.335N        P=0.676N        P=0.438N       |       (e)             (e)             (e)          |
|LOGISTIC REGRESSION    |     P=0.227N        P=0.535N        P=0.335N       |       (e)             (e)             (e)          |
|COCHRAN-ARMITAGE       |     P=0.223N                                       |       (e)                                          |
|FISHER EXACT           |                     P=0.500N        P=0.309N       |                       (e)             (e)          |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE   5
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatoblastoma                                                                                                              |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/60 (0%)       3/60 (5%)       5/60 (8%)      |     0/60 (0%)       0/60 (0%)       2/60 (3%)      |
|ADJUSTED (b)           |      0.0%            8.2%           16.8%          |      0.0%            0.0%            6.1%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     0/40 (0%)       0/22 (0%)       2/23 (9%)      |     0/39 (0%)       0/34 (0%)       2/33 (6%)      |
|FIRST INCIDENCE (DAYS) |      ---             572             633           |      ---             ---             729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.011 *       P=0.090         P=0.011 *      |     P=0.082           (e)           P=0.202        |
|INCIDENTAL TUMOR       |     P=0.049 *       P=0.542         P=0.069        |     P=0.082           (e)           P=0.202        |
|LOGISTIC REGRESSION    |     P=0.018 *       P=0.127         P=0.022 *      |     P=0.082           (e)           P=0.202        |
|COCHRAN-ARMITAGE       |     P=0.023 *                                      |     P=0.096                                        |
|FISHER EXACT           |                     P=0.122         P=0.029 *      |                       (e)           P=0.248        |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Adenoma                                                                                                      |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    10/60 (17%)     40/60 (67%)     43/60 (72%)     |     6/60 (10%)     47/60 (78%)     56/60 (93%)     |
|ADJUSTED (b)           |     24.3%           94.9%           95.4%          |     15.4%           95.9%          100.0%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       2/9 (22%)       4/10 (40%)     |     0/10 (0%)       2/10 (20%)      7/10 (70%)     |
|TERMINAL (d)           |     9/40 (23%)     20/22 (91%)     21/23 (91%)     |     6/39 (15%)     32/34 (94%)     33/33 (100%)    |
|FIRST INCIDENCE (DAYS) |      723             451             458 (I)       |      729 (T)         442             455 (I)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|INCIDENTAL TUMOR       |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|LOGISTIC REGRESSION    |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|COCHRAN-ARMITAGE       |     P<0.001 **                                     |     P<0.001 **                                     |
|FISHER EXACT           |                     P<0.001 **      P<0.001 **     |                     P<0.001 **      P<0.001 **     |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE   6
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma                                                                                                    |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     9/60 (15%)     19/60 (32%)     21/60 (35%)     |     0/60 (0%)      23/60 (38%)     28/60 (47%)     |
|ADJUSTED (b)           |     20.9%           58.8%           58.4%          |      0.0%           57.2%           61.5%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       1/9 (11%)       0/10 (0%)      |     0/10 (0%)       0/10 (0%)       1/10 (10%)     |
|TERMINAL (d)           |     7/40 (18%)     10/22 (45%)      9/23 (39%)     |     0/39 (0%)      17/34 (50%)     16/33 (48%)     |
|FIRST INCIDENCE (DAYS) |      445             458 (I)         535           |      ---             503             455 (I)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|INCIDENTAL TUMOR       |     P=0.006 **      P=0.013 *       P=0.022 *      |     P<0.001 **      P<0.001 **      P<0.001 **     |
|LOGISTIC REGRESSION    |     P=0.002 **      P=0.007 **      P=0.002 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|COCHRAN-ARMITAGE       |     P=0.009 **                                     |     P<0.001 **                                     |
|FISHER EXACT           |                     P=0.025 *       P=0.010 **     |                     P<0.001 **      P<0.001 **     |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma or Hepatoblastoma                                                                                  |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     9/60 (15%)     21/60 (35%)     24/60 (40%)     |     0/60 (0%)      23/60 (38%)     28/60 (47%)     |
|ADJUSTED (b)           |     20.9%           60.9%           65.3%          |      0.0%           57.2%           61.5%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       1/9 (11%)       0/10 (0%)      |     0/10 (0%)       0/10 (0%)       1/10 (10%)     |
|TERMINAL (d)           |     7/40 (18%)     10/22 (45%)     11/23 (48%)     |     0/39 (0%)      17/34 (50%)     16/33 (48%)     |
|FIRST INCIDENCE (DAYS) |      445             458 (I)         535           |      ---             503             455 (I)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|INCIDENTAL TUMOR       |     P=0.001 **      P=0.012 *       P=0.004 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|LOGISTIC REGRESSION    |     P=0.001 **      P=0.003 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|COCHRAN-ARMITAGE       |     P=0.002 **                                     |     P<0.001 **                                     |
|FISHER EXACT           |                     P=0.010 **      P=0.002 **     |                     P<0.001 **      P<0.001 **     |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE   7
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma or Hepatocellular Adenoma                                                                          |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    18/60 (30%)     46/60 (77%)     46/60 (77%)     |     6/60 (10%)     48/60 (80%)     58/60 (97%)     |
|ADJUSTED (b)           |     41.5%           97.8%           97.8%          |     15.4%           97.9%          100.0%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       3/9 (33%)       4/10 (40%)     |     0/10 (0%)       2/10 (20%)      8/10 (80%)     |
|TERMINAL (d)           |    15/40 (38%)     21/22 (95%)     22/23 (96%)     |     6/39 (15%)     33/34 (97%)     33/33 (100%)    |
|FIRST INCIDENCE (DAYS) |      445             451             458 (I)       |      729 (T)         442             455 (I)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|INCIDENTAL TUMOR       |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|LOGISTIC REGRESSION    |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|COCHRAN-ARMITAGE       |     P<0.001 **                                     |     P<0.001 **                                     |
|FISHER EXACT           |                     P<0.001 **      P<0.001 **     |                     P<0.001 **      P<0.001 **     |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma                                                         |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    18/60 (30%)     46/60 (77%)     46/60 (77%)     |     6/60 (10%)     48/60 (80%)     58/60 (97%)     |
|ADJUSTED (b)           |     41.5%           97.8%           97.8%          |     15.4%           97.9%          100.0%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       3/9 (33%)       4/10 (40%)     |     0/10 (0%)       2/10 (20%)      8/10 (80%)     |
|TERMINAL (d)           |    15/40 (38%)     21/22 (95%)     22/23 (96%)     |     6/39 (15%)     33/34 (97%)     33/33 (100%)    |
|FIRST INCIDENCE (DAYS) |      445             451             458 (I)       |      729 (T)         442             455 (I)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|INCIDENTAL TUMOR       |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|LOGISTIC REGRESSION    |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|COCHRAN-ARMITAGE       |     P<0.001 **                                     |     P<0.001 **                                     |
|FISHER EXACT           |                     P<0.001 **      P<0.001 **     |                     P<0.001 **      P<0.001 **     |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE   8
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Adenoma                                                                                                |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     7/60 (12%)     29/60 (48%)     29/60 (48%)     |     4/60 (7%)      20/60 (33%)     15/59 (25%)     |
|ADJUSTED (b)           |     16.5%           72.5%           69.4%          |      9.8%           48.4%           35.7%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       3/9 (33%)       5/10 (50%)     |     0/10 (0%)       3/10 (30%)      2/10 (20%)     |
|TERMINAL (d)           |     6/40 (15%)     12/22 (55%)     12/23 (52%)     |     3/39 (8%)      14/34 (41%)      9/33 (27%)     |
|FIRST INCIDENCE (DAYS) |      445             458 (I)         248           |      685             455 (I)         455 (I)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P<0.001 **      P<0.001 **      P<0.001 **     |     P=0.004 **      P<0.001 **      P=0.003 **     |
|INCIDENTAL TUMOR       |     P<0.001 **      P<0.001 **      P<0.001 **     |     P=0.010 *       P<0.001 **      P=0.008 **     |
|LOGISTIC REGRESSION    |     P<0.001 **      P<0.001 **      P<0.001 **     |     P=0.008 **      P<0.001 **      P=0.005 **     |
|COCHRAN-ARMITAGE       |     P<0.001 **                                     |     P=0.009 **                                     |
|FISHER EXACT           |                     P<0.001 **      P<0.001 **     |                     P<0.001 **      P=0.005 **     |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Carcinoma                                                                                              |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     3/60 (5%)       4/60 (7%)       1/60 (2%)      |     0/60 (0%)       0/60 (0%)       2/59 (3%)      |
|ADJUSTED (b)           |      6.6%           15.9%            3.0%          |      0.0%            0.0%            5.5%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     2/40 (5%)       2/22 (9%)       0/23 (0%)      |     0/39 (0%)       0/34 (0%)       1/33 (3%)      |
|FIRST INCIDENCE (DAYS) |      393             673             648           |      ---             ---             664           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.454N        P=0.258         P=0.441N       |     P=0.085           (e)           P=0.211        |
|INCIDENTAL TUMOR       |     P=0.230N        P=0.483         P=0.292N       |     P=0.094           (e)           P=0.252        |
|LOGISTIC REGRESSION    |     P=0.295N        P=0.449         P=0.296N       |     P=0.092           (e)           P=0.229        |
|COCHRAN-ARMITAGE       |     P=0.253N                                       |     P=0.094                                        |
|FISHER EXACT           |                     P=0.500         P=0.309N       |                       (e)           P=0.244        |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE   9
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma                                                              |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    10/60 (17%)     31/60 (52%)     30/60 (50%)     |     4/60 (7%)      20/60 (33%)     17/59 (29%)     |
|ADJUSTED (b)           |     22.7%           76.3%           70.3%          |      9.8%           48.4%           39.9%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       3/9 (33%)       5/10 (50%)     |     0/10 (0%)       3/10 (30%)      2/10 (20%)     |
|TERMINAL (d)           |     8/40 (20%)     13/22 (59%)     12/23 (52%)     |     3/39 (8%)      14/34 (41%)     10/33 (30%)     |
|FIRST INCIDENCE (DAYS) |      393             458 (I)         248           |      685             455 (I)         455 (I)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P<0.001 **      P<0.001 **      P<0.001 **     |     P=0.001 **      P<0.001 **      P=0.001 **     |
|INCIDENTAL TUMOR       |     P<0.001 **      P<0.001 **      P<0.001 **     |     P=0.003 **      P<0.001 **      P=0.003 **     |
|LOGISTIC REGRESSION    |     P<0.001 **      P<0.001 **      P<0.001 **     |     P=0.003 **      P<0.001 **      P=0.002 **     |
|COCHRAN-ARMITAGE       |     P<0.001 **                                     |     P=0.003 **                                     |
|FISHER EXACT           |                     P<0.001 **      P<0.001 **     |                     P<0.001 **      P=0.001 **     |
|=================================================================================================================================|
|                                                                                                                                 |
|Pituitary Gland: Pars Distalis or Unspecified Site                                                                               |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/52 (0%)       2/54 (4%)       0/56 (0%)      |     1/51 (2%)       9/55 (16%)      4/52 (8%)      |
|ADJUSTED (b)           |      0.0%            6.9%            0.0%          |      2.2%           27.2%           12.2%          |
|INTERIM SACRIFICE 1 (c)|     0/9 (0%)        0/9 (0%)        0/9 (0%)       |     0/8 (0%)        0/10 (0%)       0/9 (0%)       |
|TERMINAL (d)           |     0/36 (0%)       1/19 (5%)       0/22 (0%)      |     0/34 (0%)       7/30 (23%)      3/29 (10%)     |
|FIRST INCIDENCE (DAYS) |      ---             451             ---           |      628             671             553           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.605         P=0.173           (e)          |     P=0.145         P=0.007 **      P=0.151        |
|INCIDENTAL TUMOR       |     P=0.651N        P=0.251           (e)          |     P=0.207         P=0.009 **      P=0.246        |
|LOGISTIC REGRESSION    |     P=0.634N        P=0.268           (e)          |     P=0.167         P=0.008 **      P=0.179        |
|COCHRAN-ARMITAGE       |     P=0.653N                                       |     P=0.202                                        |
|FISHER EXACT           |                     P=0.257           (e)          |                     P=0.011 *       P=0.187        |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE  10
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin                                                                                                                             |
|     Fibroma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     2/60 (3%)       3/60 (5%)       1/60 (2%)      |     0/60 (0%)       0/60 (0%)       0/60 (0%)      |
|ADJUSTED (b)           |      5.0%           11.9%            3.8%          |      0.0%            0.0%            0.0%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     2/40 (5%)       1/22 (5%)       0/23 (0%)      |     0/39 (0%)       0/34 (0%)       0/33 (0%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         706             722           |      ---             ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.578         P=0.262         P=0.679N       |       (e)             (e)             (e)          |
|INCIDENTAL TUMOR       |     P=0.346N        P=0.497         P=0.518N       |       (e)             (e)             (e)          |
|LOGISTIC REGRESSION    |     P=0.551N        P=0.323         P=0.650N       |       (e)             (e)             (e)          |
|COCHRAN-ARMITAGE       |     P=0.400N                                       |       (e)                                          |
|FISHER EXACT           |                     P=0.500         P=0.500N       |                       (e)             (e)          |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin                                                                                                                             |
|     Fibroma, Fibrosarcoma, Sarcoma, Myxoma, Myxosarcoma, or Fibrous Histiocytoma                                                |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     6/60 (10%)     10/60 (17%)      3/60 (5%)      |     2/60 (3%)       1/60 (2%)       0/60 (0%)      |
|ADJUSTED (b)           |     14.1%           29.5%            8.7%          |      4.3%            2.9%            0.0%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     4/40 (10%)      3/22 (14%)      0/23 (0%)      |     0/39 (0%)       0/34 (0%)       0/33 (0%)      |
|FIRST INCIDENCE (DAYS) |      621             501             606           |      478             727             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.494N        P=0.053         P=0.476N       |     P=0.162N        P=0.526N        P=0.254N       |
|INCIDENTAL TUMOR       |     P=0.066N        P=0.587         P=0.046N*      |     P=0.072N        P=0.264N        P=0.140N       |
|LOGISTIC REGRESSION    |     P=0.068N        P=0.532         P=0.065N       |     P=0.142N        P=0.497N        P=0.247N       |
|COCHRAN-ARMITAGE       |     P=0.229N                                       |     P=0.142N                                       |
|FISHER EXACT           |                     P=0.211         P=0.245N       |                     P=0.500N        P=0.248N       |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE  11
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin                                                                                                                             |
|     Fibrosarcoma                                                                                                                |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     3/60 (5%)       8/60 (13%)      2/60 (3%)      |     2/60 (3%)       1/60 (2%)       0/60 (0%)      |
|ADJUSTED (b)           |      6.9%           22.8%            5.1%          |      4.3%            2.9%            0.0%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     1/40 (3%)       2/22 (9%)       0/23 (0%)      |     0/39 (0%)       0/34 (0%)       0/33 (0%)      |
|FIRST INCIDENCE (DAYS) |      621             501             606           |      478             727             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.526         P=0.037 *       P=0.638N       |     P=0.162N        P=0.526N        P=0.254N       |
|INCIDENTAL TUMOR       |     P=0.123N        P=0.567         P=0.066N       |     P=0.072N        P=0.264N        P=0.140N       |
|LOGISTIC REGRESSION    |     P=0.092N        P=0.521         P=0.530N       |     P=0.142N        P=0.497N        P=0.247N       |
|COCHRAN-ARMITAGE       |     P=0.430N                                       |     P=0.142N                                       |
|FISHER EXACT           |                     P=0.102         P=0.500N       |                     P=0.500N        P=0.248N       |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin                                                                                                                             |
|     Fibrosarcoma, Sarcoma, Myxosarcoma, or Fibrous Histiocytoma                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     4/60 (7%)       8/60 (13%)      2/60 (3%)      |     2/60 (3%)       1/60 (2%)       0/60 (0%)      |
|ADJUSTED (b)           |      9.3%           22.8%            5.1%          |      4.3%            2.9%            0.0%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     2/40 (5%)       2/22 (9%)       0/23 (0%)      |     0/39 (0%)       0/34 (0%)       0/33 (0%)      |
|FIRST INCIDENCE (DAYS) |      621             501             606           |      478             727             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.501N        P=0.066         P=0.508N       |     P=0.162N        P=0.526N        P=0.254N       |
|INCIDENTAL TUMOR       |     P=0.077N        P=0.630N        P=0.046N*      |     P=0.072N        P=0.264N        P=0.140N       |
|LOGISTIC REGRESSION    |     P=0.054N        P=0.635         P=0.381N       |     P=0.142N        P=0.497N        P=0.247N       |
|COCHRAN-ARMITAGE       |     P=0.305N                                       |     P=0.142N                                       |
|FISHER EXACT           |                     P=0.181         P=0.340N       |                     P=0.500N        P=0.248N       |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE  12
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|Spleen                                                                                                                           |
|     Mast Cell Tumor Malignant                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/60 (0%)       2/60 (3%)       0/58 (0%)      |     0/60 (0%)       0/60 (0%)       0/59 (0%)      |
|ADJUSTED (b)           |      0.0%            7.8%            0.0%          |      0.0%            0.0%            0.0%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/8 (0%)       |     0/10 (0%)       0/10 (0%)       0/9 (0%)       |
|TERMINAL (d)           |     0/40 (0%)       1/22 (5%)       0/23 (0%)      |     0/39 (0%)       0/34 (0%)       0/33 (0%)      |
|FIRST INCIDENCE (DAYS) |      ---             673             ---           |      ---             ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.555         P=0.137           (e)          |       (e)             (e)             (e)          |
|INCIDENTAL TUMOR       |     P=0.648N        P=0.256           (e)          |       (e)             (e)             (e)          |
|LOGISTIC REGRESSION    |     P=0.617         P=0.185           (e)          |       (e)             (e)             (e)          |
|COCHRAN-ARMITAGE       |     P=0.662                                        |       (e)                                          |
|FISHER EXACT           |                     P=0.248           (e)          |                       (e)             (e)          |
|=================================================================================================================================|
|                                                                                                                                 |
|Stomach, Forestomach                                                                                                             |
|     Squamous Cell Carcinoma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/60 (0%)      12/60 (20%)     14/60 (23%)     |     0/60 (0%)      12/60 (20%)     11/60 (18%)     |
|ADJUSTED (b)           |      0.0%           36.5%           38.8%          |      0.0%           30.9%           27.3%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        1/10 (10%)     |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     0/40 (0%)       4/22 (18%)      4/23 (17%)     |     0/39 (0%)       8/34 (24%)      5/33 (15%)     |
|FIRST INCIDENCE (DAYS) |      ---             505             458 (I)       |      ---             587             501           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P<0.001 **      P<0.001 **      P<0.001 **     |     P=0.001 **      P<0.001 **      P<0.001 **     |
|INCIDENTAL TUMOR       |     P<0.001 **      P=0.003 **      P=0.001 **     |     P=0.003 **      P<0.001 **      P=0.002 **     |
|LOGISTIC REGRESSION    |     P<0.001 **      P<0.001 **      P<0.001 **     |     P=0.002 **      P<0.001 **      P<0.001 **     |
|COCHRAN-ARMITAGE       |     P<0.001 **                                     |     P=0.002 **                                     |
|FISHER EXACT           |                     P<0.001 **      P<0.001 **     |                     P<0.001 **      P<0.001 **     |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE  13
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|Stomach, Forestomach                                                                                                             |
|     Squamous Cell Carcinoma or Papilloma Squamous                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/60 (0%)      19/60 (32%)     28/60 (47%)     |     2/60 (3%)      29/60 (48%)     36/60 (60%)     |
|ADJUSTED (b)           |      0.0%           61.2%           74.4%          |      5.1%           63.4%           81.2%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        1/10 (10%)     |     0/10 (0%)       4/10 (40%)      2/10 (20%)     |
|TERMINAL (d)           |     0/40 (0%)      11/22 (50%)     14/23 (61%)     |     2/39 (5%)      18/34 (53%)     25/33 (76%)     |
|FIRST INCIDENCE (DAYS) |      ---             505             458 (I)       |      729 (T)         455 (I)         455 (I)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|INCIDENTAL TUMOR       |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|LOGISTIC REGRESSION    |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|COCHRAN-ARMITAGE       |     P<0.001 **                                     |     P<0.001 **                                     |
|FISHER EXACT           |                     P<0.001 **      P<0.001 **     |                     P<0.001 **      P<0.001 **     |
|=================================================================================================================================|
|                                                                                                                                 |
|Stomach, Forestomach                                                                                                             |
|     Squamous Cell Papilloma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/60 (0%)      13/60 (22%)     16/60 (27%)     |     2/60 (3%)      20/60 (33%)     29/60 (48%)     |
|ADJUSTED (b)           |      0.0%           51.0%           55.6%          |      5.1%           45.8%           73.3%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       4/10 (40%)      2/10 (20%)     |
|TERMINAL (d)           |     0/40 (0%)      10/22 (45%)     11/23 (48%)     |     2/39 (5%)      12/34 (35%)     23/33 (70%)     |
|FIRST INCIDENCE (DAYS) |      ---             613             606           |      729 (T)         455 (I)         455 (I)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|INCIDENTAL TUMOR       |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|LOGISTIC REGRESSION    |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|COCHRAN-ARMITAGE       |     P<0.001 **                                     |     P<0.001 **                                     |
|FISHER EXACT           |                     P<0.001 **      P<0.001 **     |                     P<0.001 **      P<0.001 **     |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE  14
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|Thyroid Gland: Follicular Cell                                                                                                   |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/59 (0%)       0/59 (0%)       0/59 (0%)      |     3/60 (5%)       1/60 (2%)       2/58 (3%)      |
|ADJUSTED (b)           |      0.0%            0.0%            0.0%          |      7.7%            2.9%            6.1%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     0/39 (0%)       0/22 (0%)       0/23 (0%)      |     3/39 (8%)       1/34 (3%)       2/33 (6%)      |
|FIRST INCIDENCE (DAYS) |      ---             ---             ---           |      729 (T)         729 (T)         729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |       (e)             (e)             (e)          |     P=0.467N        P=0.355N        P=0.576N       |
|INCIDENTAL TUMOR       |       (e)             (e)             (e)          |     P=0.467N        P=0.355N        P=0.576N       |
|LOGISTIC REGRESSION    |       (e)             (e)             (e)          |     P=0.467N        P=0.355N        P=0.576N       |
|COCHRAN-ARMITAGE       |       (e)                                          |     P=0.413N                                       |
|FISHER EXACT           |                       (e)             (e)          |                     P=0.309N        P=0.516N       |
|=================================================================================================================================|
|                                                                                                                                 |
|Uterus                                                                                                                           |
|     Leiomyoma                                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |     2/60 (3%)       0/60 (0%)       0/60 (0%)      |
|ADJUSTED (b)           |                                                    |      5.1%            0.0%            0.0%          |
|INTERIM SACRIFICE 1 (c)|                                                    |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |                                                    |     2/39 (5%)       0/34 (0%)       0/33 (0%)      |
|FIRST INCIDENCE (DAYS) |                                                    |      729 (T)         ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |     P=0.115N        P=0.269N        P=0.276N       |
|INCIDENTAL TUMOR       |                                                    |     P=0.115N        P=0.269N        P=0.276N       |
|LOGISTIC REGRESSION    |                                                    |     P=0.115N        P=0.269N        P=0.276N       |
|COCHRAN-ARMITAGE       |                                                    |     P=0.096N                                       |
|FISHER EXACT           |                                                    |                     P=0.248N        P=0.248N       |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE  15
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|Uterus                                                                                                                           |
|     Polyp Stromal                                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |     0/60 (0%)       2/60 (3%)       0/60 (0%)      |
|ADJUSTED (b)           |                                                    |      0.0%            4.6%            0.0%          |
|INTERIM SACRIFICE 1 (c)|                                                    |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |                                                    |     0/39 (0%)       0/34 (0%)       0/33 (0%)      |
|FIRST INCIDENCE (DAYS) |                                                    |      ---             587             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |     P=0.647         P=0.225           (e)          |
|INCIDENTAL TUMOR       |                                                    |     P=0.529N        P=0.245           (e)          |
|LOGISTIC REGRESSION    |                                                    |     P=0.668         P=0.240           (e)          |
|COCHRAN-ARMITAGE       |                                                    |     P=0.668                                        |
|FISHER EXACT           |                                                    |                     P=0.248           (e)          |
|=================================================================================================================================|
|                                                                                                                                 |
|Uterus                                                                                                                           |
|     Sarcoma Stromal                                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |     0/60 (0%)       3/60 (5%)       0/60 (0%)      |
|ADJUSTED (b)           |                                                    |      0.0%            8.1%            0.0%          |
|INTERIM SACRIFICE 1 (c)|                                                    |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |                                                    |     0/39 (0%)       2/34 (6%)       0/33 (0%)      |
|FIRST INCIDENCE (DAYS) |                                                    |      ---             671             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |     P=0.597         P=0.105           (e)          |
|INCIDENTAL TUMOR       |                                                    |     P=0.633         P=0.134           (e)          |
|LOGISTIC REGRESSION    |                                                    |     P=0.625         P=0.113           (e)          |
|COCHRAN-ARMITAGE       |                                                    |     P=0.639                                        |
|FISHER EXACT           |                                                    |                     P=0.122           (e)          |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE  16
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|Uterus                                                                                                                           |
|     Sarcoma Stromal or Polyp Stromal                                                                                            |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |     0/60 (0%)       5/60 (8%)       0/60 (0%)      |
|ADJUSTED (b)           |                                                    |      0.0%           12.4%            0.0%          |
|INTERIM SACRIFICE 1 (c)|                                                    |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |                                                    |     0/39 (0%)       2/34 (6%)       0/33 (0%)      |
|FIRST INCIDENCE (DAYS) |                                                    |      ---             587             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |     P=0.562         P=0.029 *         (e)          |
|INCIDENTAL TUMOR       |                                                    |     P=0.525N        P=0.038 *         (e)          |
|LOGISTIC REGRESSION    |                                                    |     P=0.605         P=0.032 *         (e)          |
|COCHRAN-ARMITAGE       |                                                    |     P=0.609                                        |
|FISHER EXACT           |                                                    |                     P=0.029 *         (e)          |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangioma                                                                                                                  |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/60 (0%)       0/60 (0%)       0/60 (0%)      |     4/60 (7%)       0/60 (0%)       1/60 (2%)      |
|ADJUSTED (b)           |      0.0%            0.0%            0.0%          |      9.6%            0.0%            3.0%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     0/40 (0%)       0/22 (0%)       0/23 (0%)      |     3/39 (8%)       0/34 (0%)       1/33 (3%)      |
|FIRST INCIDENCE (DAYS) |      ---             ---             ---           |      478             ---             729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |       (e)             (e)             (e)          |     P=0.105N        P=0.080N        P=0.222N       |
|INCIDENTAL TUMOR       |       (e)             (e)             (e)          |     P=0.083N        P=0.042N*       P=0.178N       |
|LOGISTIC REGRESSION    |       (e)             (e)             (e)          |     P=0.084N        P=0.064N        P=0.183N       |
|COCHRAN-ARMITAGE       |       (e)                                          |     P=0.082N                                       |
|FISHER EXACT           |                       (e)             (e)          |                     P=0.059N        P=0.182N       |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE  17
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangiosarcoma                                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     3/60 (5%)       2/60 (3%)       2/60 (3%)      |     0/60 (0%)       0/60 (0%)       0/60 (0%)      |
|ADJUSTED (b)           |      7.5%            6.2%            4.4%          |      0.0%            0.0%            0.0%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     3/40 (8%)       1/22 (5%)       0/23 (0%)      |     0/39 (0%)       0/34 (0%)       0/33 (0%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         414             484           |      ---             ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.560N        P=0.655         P=0.650N       |       (e)             (e)             (e)          |
|INCIDENTAL TUMOR       |     P=0.519N        P=0.676N        P=0.610N       |       (e)             (e)             (e)          |
|LOGISTIC REGRESSION    |     P=0.395N        P=0.535N        P=0.506N       |       (e)             (e)             (e)          |
|COCHRAN-ARMITAGE       |     P=0.407N                                       |       (e)                                          |
|FISHER EXACT           |                     P=0.500N        P=0.500N       |                       (e)             (e)          |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangiosarcoma or Hemangioma                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     3/60 (5%)       2/60 (3%)       2/60 (3%)      |     4/60 (7%)       0/60 (0%)       1/60 (2%)      |
|ADJUSTED (b)           |      7.5%            6.2%            4.4%          |      9.6%            0.0%            3.0%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     3/40 (8%)       1/22 (5%)       0/23 (0%)      |     3/39 (8%)       0/34 (0%)       1/33 (3%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         414             484           |      478             ---             729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.560N        P=0.655         P=0.650N       |     P=0.105N        P=0.080N        P=0.222N       |
|INCIDENTAL TUMOR       |     P=0.519N        P=0.676N        P=0.610N       |     P=0.083N        P=0.042N*       P=0.178N       |
|LOGISTIC REGRESSION    |     P=0.395N        P=0.535N        P=0.506N       |     P=0.084N        P=0.064N        P=0.183N       |
|COCHRAN-ARMITAGE       |     P=0.407N                                       |     P=0.082N                                       |
|FISHER EXACT           |                     P=0.500N        P=0.500N       |                     P=0.059N        P=0.182N       |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE  18
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Lymphoma And Histiocytic Sarcoma                                                                                  |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    10/60 (17%)      7/60 (12%)      8/60 (13%)     |    11/60 (18%)     15/60 (25%)     13/60 (22%)     |
|ADJUSTED (b)           |     21.9%           23.2%           25.8%          |     25.5%           36.6%           35.7%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     5/40 (13%)      2/22 (9%)       4/23 (17%)     |     7/39 (18%)     10/34 (29%)     10/33 (30%)     |
|FIRST INCIDENCE (DAYS) |      480             612             512           |      653             202             680           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.397         P=0.524         P=0.447        |     P=0.237         P=0.181         P=0.268        |
|INCIDENTAL TUMOR       |     P=0.222N        P=0.081N        P=0.262N       |     P=0.275         P=0.330         P=0.365        |
|LOGISTIC REGRESSION    |     P=0.229N        P=0.119N        P=0.211N       |     P=0.277         P=0.230         P=0.313        |
|COCHRAN-ARMITAGE       |     P=0.346N                                       |     P=0.370                                        |
|FISHER EXACT           |                     P=0.301N        P=0.399N       |                     P=0.253         P=0.410        |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Lymphoma: Histiocytic, Lymphocytic, Mixed, NOS, or Undifferentiated                                               |
|     Cell Type                                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    10/60 (17%)      6/60 (10%)      7/60 (12%)     |    11/60 (18%)     15/60 (25%)     13/60 (22%)     |
|ADJUSTED (b)           |     21.9%           20.8%           23.3%          |     25.5%           36.6%           35.7%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       0/9 (0%)        0/10 (0%)      |     0/10 (0%)       0/10 (0%)       0/10 (0%)      |
|TERMINAL (d)           |     5/40 (13%)      2/22 (9%)       4/23 (17%)     |     7/39 (18%)     10/34 (29%)     10/33 (30%)     |
|FIRST INCIDENCE (DAYS) |      480             612             512           |      653             202             680           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.517         P=0.565N        P=0.567        |     P=0.237         P=0.181         P=0.268        |
|INCIDENTAL TUMOR       |     P=0.183N        P=0.073N        P=0.211N       |     P=0.275         P=0.330         P=0.365        |
|LOGISTIC REGRESSION    |     P=0.183N        P=0.294N        P=0.157N       |     P=0.277         P=0.230         P=0.313        |
|COCHRAN-ARMITAGE       |     P=0.247N                                       |     P=0.370                                        |
|FISHER EXACT           |                     P=0.211N        P=0.301N       |                     P=0.253         P=0.410        |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE  19
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Benign Tumors                                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    20/60 (33%)     47/60 (78%)     50/60 (83%)     |    24/60 (40%)     51/60 (85%)     58/60 (97%)     |
|ADJUSTED (b)           |     47.2%          100.0%          100.0%          |     54.1%           96.2%          100.0%          |
|INTERIM SACRIFICE 1 (c)|     0/10 (0%)       4/9 (44%)       7/10 (70%)     |     0/10 (0%)       5/10 (50%)      8/10 (80%)     |
|TERMINAL (d)           |    18/40 (45%)     22/22 (100%)    23/23 (100%)    |    19/39 (49%)     32/34 (94%)     33/33 (100%)    |
|FIRST INCIDENCE (DAYS) |      445             451             248           |      478             442             455 (I)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|INCIDENTAL TUMOR       |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|LOGISTIC REGRESSION    |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|COCHRAN-ARMITAGE       |     P<0.001 **                                     |     P<0.001 **                                     |
|FISHER EXACT           |                     P<0.001 **      P<0.001 **     |                     P<0.001 **      P<0.001 **     |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Tumors                                                                                                            |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    26/60 (43%)     40/60 (67%)     40/60 (67%)     |    14/60 (23%)     40/60 (67%)     38/60 (63%)     |
|ADJUSTED (b)           |     51.5%           83.1%           88.5%          |     30.9%           84.8%           78.9%          |
|INTERIM SACRIFICE 1 (c)|     1/10 (10%)      1/9 (11%)       1/10 (10%)     |     0/10 (0%)       0/10 (0%)       1/10 (10%)     |
|TERMINAL (d)           |    16/40 (40%)     14/22 (64%)     18/23 (78%)     |     8/39 (21%)     27/34 (79%)     23/33 (70%)     |
|FIRST INCIDENCE (DAYS) |      393             414             458 (I)       |      478             202             455 (I)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|INCIDENTAL TUMOR       |     P=0.011 *       P=0.171         P=0.016 *      |     P<0.001 **      P<0.001 **      P<0.001 **     |
|LOGISTIC REGRESSION    |     P=0.019 *       P=0.067         P=0.030 *      |     P<0.001 **      P<0.001 **      P<0.001 **     |
|COCHRAN-ARMITAGE       |     P=0.006 **                                     |     P<0.001 **                                     |
|FISHER EXACT           |                     P=0.008 **      P=0.008 **     |                     P<0.001 **      P<0.001 **     |
|=================================================================================================================================|
         DATE: 05/19/95                     EXPERIMENT: 05061 TEST: 02                                                 PAGE  20
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 1-AMINO-2,4-DIBROMOANTHRAQUINONE
                                                 TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      UNTREATE        1.0%            2.0%          |      UNTREATE        1.0%            2.0%          |
|                       |      D 71UM          71LM            71HM          |      D 71UF          71LF            71HF          |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant and Benign Tumors                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    38/60 (63%)     53/60 (88%)     55/60 (92%)     |    32/60 (53%)     55/60 (92%)     59/60 (98%)     |
|ADJUSTED (b)           |     75.8%          100.0%          100.0%          |     66.7%          100.0%          100.0%          |
|INTERIM SACRIFICE 1 (c)|     1/10 (10%)      4/9 (44%)       8/10 (80%)     |     0/10 (0%)       5/10 (50%)      9/10 (90%)     |
|TERMINAL (d)           |    28/40 (70%)     22/22 (100%)    23/23 (100%)    |    23/39 (59%)     34/34 (100%)    33/33 (100%)    |
|FIRST INCIDENCE (DAYS) |      393             414             248           |      478             202             455 (I)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|INCIDENTAL TUMOR       |     P<0.001 **      P=0.004 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|LOGISTIC REGRESSION    |     P<0.001 **      P<0.001 **      P<0.001 **     |     P<0.001 **      P<0.001 **      P<0.001 **     |
|COCHRAN-ARMITAGE       |     P<0.001 **                                     |     P<0.001 **                                     |
|FISHER EXACT           |                     P=0.001 **      P<0.001 **     |                     P<0.001 **      P<0.001 **     |
|=================================================================================================================================|
 (a)  Number of tumor-bearing animals / number of animals examined at site.
 (b)  Kaplan-Meier estimated lifetime tumor incidence after adjustment for 
      intercurrent mortality.
 (c)  Observed incidence at interim kill.
          Male interim sacrifices(days):   458
          Female interim sacrifices(days): 455
 (d)  Observed incidence at terminal kill.
 (f)  Beneath the control incidence are the P-values associated with the trend 
      test.  Beneath the dosed group incidence are the P-values corresponding to 
      pairwise comparisons between the controls and that dosed group.  The life
      table analysis regards tumors in animals dying  prior to terminal kill as
      being (directly or indirectly) the cause of death.  The incidental tumor 
      test regards these lesions as non-fatal.  Logistic regression is an alternative
      method for analyzing the incidence of non-fatal tumors.  The Cochran-Armitage
      and Fisher's exact tests compare directly the overall incidence rates.  
      For all tests, a negative trend is indicated by "N".
 (e)  Value of Statistic cannot be computed. 
 (I)  Interim sacrifice
 (T)  Terminal sacrifice
  #   Tumor rates based on number of animals necropsied.
  *   To the right of any statistical result, indicates significance at (P<=0.05).
  **  To the right of any statistical result, indicates significance at (P<=0.01).

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