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TR 396 Mouse Pathology Tables

NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: IRDC                                                 MONOCHLOROACETIC ACID                                DATE: 09/18/94       
EXPERIMENT: 05705 TEST: 01                                                                                     TIME: 19:59:13       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                   PAGE:       1        
CONT: 05700-05              ROUTE: GAVAGE                                                                      NTP C#: 60231        
PATHOLOGIST: D. GOODMAN                      S. EUSTIS                                                         CAS: 79-11-8         
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               REASONS FOR REMOVAL:    ALL                                                                                          
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
               REMOVAL DATE RANGE:     ALL                                                                                          
                                                                                                                                    
               TREATMENT GROUPS:       INCLUDE ALL                                                                                  
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08    
LAB: IRDC                                                 MONOCHLOROACETIC ACID                                DATE: 09/18/94      
EXPERIMENT: 05705 TEST: 01                                                                                     TIME: 19:59:13      
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                       
CONT: 05700-05              ROUTE: GAVAGE                                                                      NTP C#: 60231       
PATHOLOGIST: D. GOODMAN                      S. EUSTIS                                                         CAS: 79-11-8        
 
 
 
 
 
                                                    MICE(B6C3F1)
                                              ----------------------------
             FOR ALL DOSES THE TUMOR RATES IN THE FOLLOWING TISSUES/ORGANS ARE BASED ON NUMBER OF TISSUES EXAMINED.
             IN OTHER TISSUES/ORGANS, RATES ARE BASED ON NUMBER OF ANIMALS NECROPSIED.
 
 
                                                    ----------------
 
                                                    Adrenal Gland
                                                    Bone Marrow
                                                    Brain
                                                    Clitoral Gland
                                                    Epididymis
                                                    Gallbladder
                                                    Heart
                                                    Islets, Pancreatic
                                                    Kidney
                                                    Liver
                                                    Lung
                                                    Nose
                                                    Ovary
                                                    Pancreas
                                                    Parathyroid Gland
                                                    Pituitary Gland
                                                    Preputial Gland
                                                    Prostate
                                                    Salivary Glands
                                                    Spleen
                                                    Testes
                                                    Thymus
                                                    Thyroid Gland
                                                    Urinary Bladder
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: IRDC                                                 MONOCHLOROACETIC ACID                                DATE: 09/18/94       
EXPERIMENT: 05705 TEST: 01                                                                                     TIME: 19:59:13       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                        
CONT: 05700-05              ROUTE: GAVAGE                                                                      NTP C#: 60231        
PATHOLOGIST: D. GOODMAN                      S. EUSTIS                                                         CAS: 79-11-8         
 
 
 
 
 
                                     SUMMARY OF STATISTICALLY SIGNIFICANT (P<=.05) RESULTS
                                     IN THE ANALYSIS OF THE STUDY OF MONOCHLOROACETIC ACID
 
              ====================================================================================================
 
                                                           MALE MICE
                                                          -------------
                                 ORGAN                               MORPHOLOGY
                                -------                             ------------
 
                             Lung                          Alveolar/Bronchiolar Carcinoma
                                                           Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma
                             Skin                          Neurofibrosarcoma
                             All Organs                    Benign Tumors
                                                           Malignant and Benign Tumors
 
              ====================================================================================================
 
                                                           FEMALE MICE
                                                          -------------
                                 ORGAN                               MORPHOLOGY
                                -------                             ------------
 
                             Lung                          Alveolar/Bronchiolar Adenoma
                                                           Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma
                             Pituitary Gland: Pars Distalis or Unspecified Site
                                                           Adenoma
                             Thyroid Gland: Follicular Cell
                                                           Adenoma
                                                           Carcinoma or Adenoma
                             All Organs                    Malignant Lymphoma And Histiocytic Sarcoma
                                                           Malignant Lymphoma: Histiocytic, Lymphocytic, Mixed,
                                                            NOS, or Undifferentiated Cell Type
                                                           Malignant Tumors
                                                           Malignant and Benign Tumors
 
              ====================================================================================================
         DATE: 09/18/94                     EXPERIMENT: 05705 TEST: 01                                                 PAGE   1
                            STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      CONTROL         50 MG/KG        100MG/KG      |      CONTROL         50 MG/KG        100MG/KG      |
|                       |      MALE            MALE            MALE          |      FEMALE          FEMALE          FEMALE        |
|=================================================================================================================================|
|                                                                                                                                 |
|Adrenal Cortex                                                                                                                   |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     2/60 (3%)       2/59 (3%)       2/60 (3%)      |     1/59 (2%)       0/1 (0%)        0/60 (0%)      |
|ADJUSTED (b)           |      4.3%            5.1%            9.5%          |      2.4%            0.0%            0.0%          |
|TERMINAL (d)           |     2/46 (4%)       2/39 (5%)       2/21 (10%)     |     1/41 (2%)       0/0             0/44 (0%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         729 (T)         729 (T)       |      729 (T)         ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.307         P=0.634         P=0.393        |     P=0.296N          (e)           P=0.486N       |
|INCIDENTAL TUMOR       |     P=0.307         P=0.634         P=0.393        |     P=0.296N          (e)           P=0.486N       |
|LOGISTIC REGRESSION    |     P=0.307         P=0.634         P=0.393        |     P=0.296N          (e)           P=0.486N       |
|COCHRAN-ARMITAGE       |     P=0.600                                        |     P=0.304N                                       |
|FISHER EXACT           |                     P=0.684         P=0.691N       |                     P=0.983N        P=0.496N       |
|=================================================================================================================================|
|                                                                                                                                 |
|Harderian Gland                                                                                                                  |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     2/60 (3%)       3/60 (5%)       2/60 (3%)      |     1/60 (2%)       0/60 (0%)       0/60 (0%)      |
|ADJUSTED (b)           |      4.3%            7.2%            7.1%          |      2.4%            0.0%            0.0%          |
|TERMINAL (d)           |     2/46 (4%)       2/39 (5%)       1/21 (5%)      |     1/42 (2%)       0/40 (0%)       0/44 (0%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         670             467           |      729 (T)         ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.312         P=0.433         P=0.442        |     P=0.266N        P=0.510N        P=0.491N       |
|INCIDENTAL TUMOR       |     P=0.516         P=0.470         P=0.650        |     P=0.266N        P=0.510N        P=0.491N       |
|LOGISTIC REGRESSION    |     P=0.458         P=0.451         P=0.609        |     P=0.266N        P=0.510N        P=0.491N       |
|COCHRAN-ARMITAGE       |     P=0.593                                        |     P=0.270N                                       |
|FISHER EXACT           |                     P=0.500         P=0.691N       |                     P=0.500N        P=0.500N       |
|=================================================================================================================================|
 
         DATE: 09/18/94                     EXPERIMENT: 05705 TEST: 01                                                 PAGE   2
                            STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      CONTROL         50 MG/KG        100MG/KG      |      CONTROL         50 MG/KG        100MG/KG      |
|                       |      MALE            MALE            MALE          |      FEMALE          FEMALE          FEMALE        |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hemangioma                                                                                                                  |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     2/60 (3%)       0/59 (0%)       0/59 (0%)      |     0/60 (0%)       0/60 (0%)       0/60 (0%)      |
|ADJUSTED (b)           |      4.3%            0.0%            0.0%          |      0.0%            0.0%            0.0%          |
|TERMINAL (d)           |     2/46 (4%)       0/39 (0%)       0/21 (0%)      |     0/42 (0%)       0/40 (0%)       0/44 (0%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         ---             ---           |      ---             ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.168N        P=0.276N        P=0.423N       |       (e)             (e)             (e)          |
|INCIDENTAL TUMOR       |     P=0.168N        P=0.276N        P=0.423N       |       (e)             (e)             (e)          |
|LOGISTIC REGRESSION    |     P=0.168N        P=0.276N        P=0.423N       |       (e)             (e)             (e)          |
|COCHRAN-ARMITAGE       |     P=0.098N                                       |       (e)                                          |
|FISHER EXACT           |                     P=0.252N        P=0.252N       |                       (e)             (e)          |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Adenoma                                                                                                      |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     6/60 (10%)      6/59 (10%)      1/59 (2%)      |     1/60 (2%)       1/60 (2%)       2/60 (3%)      |
|ADJUSTED (b)           |     12.7%           14.8%            4.2%          |      2.4%            2.5%            4.5%          |
|TERMINAL (d)           |     5/46 (11%)      5/39 (13%)      0/21 (0%)      |     1/42 (2%)       1/40 (3%)       2/44 (5%)      |
|FIRST INCIDENCE (DAYS) |      702             673             695           |      729 (T)         729 (T)         729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.285N        P=0.505         P=0.274N       |     P=0.395         P=0.751         P=0.516        |
|INCIDENTAL TUMOR       |     P=0.189N        P=0.562         P=0.169N       |     P=0.395         P=0.751         P=0.516        |
|LOGISTIC REGRESSION    |     P=0.224N        P=0.535         P=0.220N       |     P=0.395         P=0.751         P=0.516        |
|COCHRAN-ARMITAGE       |     P=0.059N                                       |     P=0.378                                        |
|FISHER EXACT           |                     P=0.607         P=0.060N       |                     P=0.752N        P=0.500        |
|=================================================================================================================================|
 
         DATE: 09/18/94                     EXPERIMENT: 05705 TEST: 01                                                 PAGE   3
                            STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      CONTROL         50 MG/KG        100MG/KG      |      CONTROL         50 MG/KG        100MG/KG      |
|                       |      MALE            MALE            MALE          |      FEMALE          FEMALE          FEMALE        |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma                                                                                                    |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     6/60 (10%)      2/59 (3%)       5/59 (8%)      |     0/60 (0%)       1/60 (2%)       0/60 (0%)      |
|ADJUSTED (b)           |     11.7%            4.7%           19.9%          |      0.0%            2.5%            0.0%          |
|TERMINAL (d)           |     3/46 (7%)       1/39 (3%)       3/21 (14%)     |     0/42 (0%)       1/40 (3%)       0/44 (0%)      |
|FIRST INCIDENCE (DAYS) |      517             670             581           |      ---             729 (T)         ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.322         P=0.188N        P=0.292        |     P=0.721N        P=0.490           (e)          |
|INCIDENTAL TUMOR       |     P=0.517N        P=0.132N        P=0.639        |     P=0.721N        P=0.490           (e)          |
|LOGISTIC REGRESSION    |     P=0.530N        P=0.146N        P=0.617N       |     P=0.721N        P=0.490           (e)          |
|COCHRAN-ARMITAGE       |     P=0.440N                                       |     P=0.730                                        |
|FISHER EXACT           |                     P=0.142N        P=0.512N       |                     P=0.500           (e)          |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma or Hepatocellular Adenoma                                                                          |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    12/60 (20%)      8/59 (14%)      6/59 (10%)     |     1/60 (2%)       2/60 (3%)       2/60 (3%)      |
|ADJUSTED (b)           |     23.6%           19.1%           23.3%          |      2.4%            5.0%            4.5%          |
|TERMINAL (d)           |     8/46 (17%)      6/39 (15%)      3/21 (14%)     |     1/42 (2%)       2/40 (5%)       2/44 (5%)      |
|FIRST INCIDENCE (DAYS) |      517             670             581           |      729 (T)         729 (T)         729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.528N        P=0.359N        P=0.581        |     P=0.408         P=0.483         P=0.516        |
|INCIDENTAL TUMOR       |     P=0.224N        P=0.266N        P=0.259N       |     P=0.408         P=0.483         P=0.516        |
|LOGISTIC REGRESSION    |     P=0.282N        P=0.295N        P=0.346N       |     P=0.408         P=0.483         P=0.516        |
|COCHRAN-ARMITAGE       |     P=0.082N                                       |     P=0.391                                        |
|FISHER EXACT           |                     P=0.244N        P=0.107N       |                     P=0.500         P=0.500        |
|=================================================================================================================================|
 
         DATE: 09/18/94                     EXPERIMENT: 05705 TEST: 01                                                 PAGE   4
                            STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      CONTROL         50 MG/KG        100MG/KG      |      CONTROL         50 MG/KG        100MG/KG      |
|                       |      MALE            MALE            MALE          |      FEMALE          FEMALE          FEMALE        |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma                                                         |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    12/60 (20%)      8/59 (14%)      6/59 (10%)     |     1/60 (2%)       2/60 (3%)       2/60 (3%)      |
|ADJUSTED (b)           |     23.6%           19.1%           23.3%          |      2.4%            5.0%            4.5%          |
|TERMINAL (d)           |     8/46 (17%)      6/39 (15%)      3/21 (14%)     |     1/42 (2%)       2/40 (5%)       2/44 (5%)      |
|FIRST INCIDENCE (DAYS) |      517             670             581           |      729 (T)         729 (T)         729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.528N        P=0.359N        P=0.581        |     P=0.408         P=0.483         P=0.516        |
|INCIDENTAL TUMOR       |     P=0.224N        P=0.266N        P=0.259N       |     P=0.408         P=0.483         P=0.516        |
|LOGISTIC REGRESSION    |     P=0.282N        P=0.295N        P=0.346N       |     P=0.408         P=0.483         P=0.516        |
|COCHRAN-ARMITAGE       |     P=0.082N                                       |     P=0.391                                        |
|FISHER EXACT           |                     P=0.244N        P=0.107N       |                     P=0.500         P=0.500        |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Adenoma                                                                                                |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     9/60 (15%)     10/59 (17%)      5/60 (8%)      |     0/60 (0%)       2/14 (14%)      2/60 (3%)      |
|ADJUSTED (b)           |     19.6%           25.6%           20.7%          |      0.0%           21.6%            4.5%          |
|TERMINAL (d)           |     9/46 (20%)     10/39 (26%)      3/21 (14%)     |     0/42 (0%)       1/5 (20%)       2/44 (5%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         729 (T)         594           |      ---             636             729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.373         P=0.342         P=0.487        |     P=0.217         P=0.060         P=0.249        |
|INCIDENTAL TUMOR       |     P=0.441         P=0.342         P=0.599        |     P=0.198         P=0.079         P=0.249        |
|LOGISTIC REGRESSION    |     P=0.462         P=0.342         P=0.602        |     P=0.192         P=0.020 *       P=0.249        |
|COCHRAN-ARMITAGE       |     P=0.174N                                       |     P=0.211                                        |
|FISHER EXACT           |                     P=0.484         P=0.197N       |                     P=0.034 *       P=0.248        |
|=================================================================================================================================|
 
         DATE: 09/18/94                     EXPERIMENT: 05705 TEST: 01                                                 PAGE   5
                            STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      CONTROL         50 MG/KG        100MG/KG      |      CONTROL         50 MG/KG        100MG/KG      |
|                       |      MALE            MALE            MALE          |      FEMALE          FEMALE          FEMALE        |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Carcinoma                                                                                              |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     4/60 (7%)       0/59 (0%)       0/60 (0%)      |     0/60 (0%)       1/14 (7%)       0/60 (0%)      |
|ADJUSTED (b)           |      8.7%            0.0%            0.0%          |      0.0%           20.0%            0.0%          |
|TERMINAL (d)           |     4/46 (9%)       0/39 (0%)       0/21 (0%)      |     0/42 (0%)       1/5 (20%)       0/44 (0%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         ---             ---           |      ---             729 (T)         ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.044N*       P=0.086N        P=0.203N       |     P=0.689N        P=0.101           (e)          |
|INCIDENTAL TUMOR       |     P=0.044N*       P=0.086N        P=0.203N       |     P=0.689N        P=0.101           (e)          |
|LOGISTIC REGRESSION    |     P=0.043N*       P=0.086N        P=0.203N       |     P=0.689N        P=0.101           (e)          |
|COCHRAN-ARMITAGE       |     P=0.015N*                                      |     P=0.702                                        |
|FISHER EXACT           |                     P=0.061N        P=0.059N       |                     P=0.189           (e)          |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma                                                              |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    12/60 (20%)     10/59 (17%)      5/60 (8%)      |     0/60 (0%)       3/14 (21%)      2/60 (3%)      |
|ADJUSTED (b)           |     26.1%           25.6%           20.7%          |      0.0%           41.2%            4.5%          |
|TERMINAL (d)           |    12/46 (26%)     10/39 (26%)      3/21 (14%)     |     0/42 (0%)       2/5 (40%)       2/44 (5%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         729 (T)         594           |      ---             636             729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.477N        P=0.579N        P=0.526N       |     P=0.246         P=0.003 **      P=0.249        |
|INCIDENTAL TUMOR       |     P=0.411N        P=0.579N        P=0.421N       |     P=0.229         P=0.004 **      P=0.249        |
|LOGISTIC REGRESSION    |     P=0.387N        P=0.579N        P=0.410N       |     P=0.213         P=0.001 **      P=0.249        |
|COCHRAN-ARMITAGE       |     P=0.049N*                                      |     P=0.235                                        |
|FISHER EXACT           |                     P=0.424N        P=0.057N       |                     P=0.006 **      P=0.248        |
|=================================================================================================================================|
 
         DATE: 09/18/94                     EXPERIMENT: 05705 TEST: 01                                                 PAGE   6
                            STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      CONTROL         50 MG/KG        100MG/KG      |      CONTROL         50 MG/KG        100MG/KG      |
|                       |      MALE            MALE            MALE          |      FEMALE          FEMALE          FEMALE        |
|=================================================================================================================================|
|                                                                                                                                 |
|Mammary Gland                                                                                                                    |
|     Carcinoma                                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/60 (0%)       0/60 (0%)       0/60 (0%)      |     2/60 (3%)       2/60 (3%)       2/60 (3%)      |
|ADJUSTED (b)           |      0.0%            0.0%            0.0%          |      4.3%            5.0%            4.5%          |
|TERMINAL (d)           |     0/46 (0%)       0/39 (0%)       0/21 (0%)      |     1/42 (2%)       2/40 (5%)       2/44 (5%)      |
|FIRST INCIDENCE (DAYS) |      ---             ---             ---           |      682             729 (T)         729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |       (e)             (e)             (e)          |     P=0.588N        P=0.669         P=0.686N       |
|INCIDENTAL TUMOR       |       (e)             (e)             (e)          |     P=0.576         P=0.652         P=0.654        |
|LOGISTIC REGRESSION    |       (e)             (e)             (e)          |     P=0.581         P=0.668         P=0.670        |
|COCHRAN-ARMITAGE       |       (e)                                          |     P=0.600                                        |
|FISHER EXACT           |                       (e)             (e)          |                     P=0.691N        P=0.691N       |
|=================================================================================================================================|
|                                                                                                                                 |
|Ovary                                                                                                                            |
|     Granulosa Cell Tumor, Benign, Malignant, NOS                                                                                |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |     1/59 (2%)       2/24 (8%)       0/60 (0%)      |
|ADJUSTED (b)           |                                                    |      2.4%            7.9%            0.0%          |
|TERMINAL (d)           |                                                    |     1/42 (2%)       1/17 (6%)       0/44 (0%)      |
|FIRST INCIDENCE (DAYS) |                                                    |      729 (T)         651             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |     P=0.369N        P=0.287         P=0.491N       |
|INCIDENTAL TUMOR       |                                                    |     P=0.417N        P=0.182         P=0.491N       |
|LOGISTIC REGRESSION    |                                                    |     P=0.389N        P=0.197         P=0.491N       |
|COCHRAN-ARMITAGE       |                                                    |     P=0.369N                                       |
|FISHER EXACT           |                                                    |                     P=0.199         P=0.496N       |
|=================================================================================================================================|
 
         DATE: 09/18/94                     EXPERIMENT: 05705 TEST: 01                                                 PAGE   7
                            STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      CONTROL         50 MG/KG        100MG/KG      |      CONTROL         50 MG/KG        100MG/KG      |
|                       |      MALE            MALE            MALE          |      FEMALE          FEMALE          FEMALE        |
|=================================================================================================================================|
|                                                                                                                                 |
|Pituitary Gland: Pars Distalis or Unspecified Site                                                                               |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/56 (0%)       0/57 (0%)       0/56 (0%)      |     5/55 (9%)       6/6 (100%)      7/57 (12%)     |
|ADJUSTED (b)           |      0.0%            0.0%            0.0%          |     11.9%          100.0%           17.1%          |
|TERMINAL (d)           |     0/43 (0%)       0/38 (0%)       0/20 (0%)      |     4/41 (10%)      5/5 (100%)      7/41 (17%)     |
|FIRST INCIDENCE (DAYS) |      ---             ---             ---           |      724             675             729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |       (e)             (e)             (e)          |     P=0.342         P<0.001 **      P=0.382        |
|INCIDENTAL TUMOR       |       (e)             (e)             (e)          |     P=0.309         P<0.001 **      P=0.356        |
|LOGISTIC REGRESSION    |       (e)             (e)             (e)          |     P=0.310         P<0.001 **      P=0.375        |
|COCHRAN-ARMITAGE       |       (e)                                          |     P=0.377                                        |
|FISHER EXACT           |                       (e)             (e)          |                     P<0.001 **      P=0.406        |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin                                                                                                                             |
|     Fibrosarcoma                                                                                                                |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     1/60 (2%)       4/60 (7%)       3/60 (5%)      |     2/60 (3%)       2/60 (3%)       1/60 (2%)      |
|ADJUSTED (b)           |      2.2%            8.8%            9.5%          |      4.6%            3.9%            2.3%          |
|TERMINAL (d)           |     1/46 (2%)       0/39 (0%)       0/21 (0%)      |     1/42 (2%)       0/40 (0%)       1/44 (2%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         581             495           |      717             545             729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.080         P=0.147         P=0.127        |     P=0.394N        P=0.664         P=0.484N       |
|INCIDENTAL TUMOR       |     P=0.410         P=0.236         P=0.433        |     P=0.570N        P=0.659         P=0.558N       |
|LOGISTIC REGRESSION    |     P=0.341         P=0.170         P=0.267        |     P=0.392N        P=0.682N        P=0.510N       |
|COCHRAN-ARMITAGE       |     P=0.253                                        |     P=0.391N                                       |
|FISHER EXACT           |                     P=0.182         P=0.309        |                     P=0.691N        P=0.500N       |
|=================================================================================================================================|
 
         DATE: 09/18/94                     EXPERIMENT: 05705 TEST: 01                                                 PAGE   8
                            STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      CONTROL         50 MG/KG        100MG/KG      |      CONTROL         50 MG/KG        100MG/KG      |
|                       |      MALE            MALE            MALE          |      FEMALE          FEMALE          FEMALE        |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin                                                                                                                             |
|     Neurofibrosarcoma                                                                                                           |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/60 (0%)       0/60 (0%)       2/60 (3%)      |     0/60 (0%)       0/60 (0%)       0/60 (0%)      |
|ADJUSTED (b)           |      0.0%            0.0%            8.0%          |      0.0%            0.0%            0.0%          |
|TERMINAL (d)           |     0/46 (0%)       0/39 (0%)       1/21 (5%)      |     0/42 (0%)       0/40 (0%)       0/44 (0%)      |
|FIRST INCIDENCE (DAYS) |      ---             ---             599           |      ---             ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.038 *         (e)           P=0.104        |       (e)             (e)             (e)          |
|INCIDENTAL TUMOR       |     P=0.074           (e)           P=0.192        |       (e)             (e)             (e)          |
|LOGISTIC REGRESSION    |     P=0.066           (e)           P=0.170        |       (e)             (e)             (e)          |
|COCHRAN-ARMITAGE       |     P=0.096                                        |       (e)                                          |
|FISHER EXACT           |                       (e)           P=0.248        |                       (e)             (e)          |
|=================================================================================================================================|
|                                                                                                                                 |
|Stomach, Forestomach                                                                                                             |
|     Squamous Cell Papilloma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/60 (0%)       0/60 (0%)       0/60 (0%)      |     0/60 (0%)       0/60 (0%)       2/60 (3%)      |
|ADJUSTED (b)           |      0.0%            0.0%            0.0%          |      0.0%            0.0%            4.5%          |
|TERMINAL (d)           |     0/46 (0%)       0/39 (0%)       0/21 (0%)      |     0/42 (0%)       0/40 (0%)       2/44 (5%)      |
|FIRST INCIDENCE (DAYS) |      ---             ---             ---           |      ---             ---             729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |       (e)             (e)             (e)          |     P=0.103           (e)           P=0.249        |
|INCIDENTAL TUMOR       |       (e)             (e)             (e)          |     P=0.103           (e)           P=0.249        |
|LOGISTIC REGRESSION    |       (e)             (e)             (e)          |     P=0.103           (e)           P=0.249        |
|COCHRAN-ARMITAGE       |       (e)                                          |     P=0.096                                        |
|FISHER EXACT           |                       (e)             (e)          |                       (e)           P=0.248        |
|=================================================================================================================================|
 
         DATE: 09/18/94                     EXPERIMENT: 05705 TEST: 01                                                 PAGE   9
                            STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      CONTROL         50 MG/KG        100MG/KG      |      CONTROL         50 MG/KG        100MG/KG      |
|                       |      MALE            MALE            MALE          |      FEMALE          FEMALE          FEMALE        |
|=================================================================================================================================|
|                                                                                                                                 |
|Thyroid Gland: Follicular Cell                                                                                                   |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     1/60 (2%)       0/58 (0%)       0/57 (0%)      |     1/60 (2%)       1/1 (100%)      2/60 (3%)      |
|ADJUSTED (b)           |      2.2%            0.0%            0.0%          |      2.4%          100.0%            4.2%          |
|TERMINAL (d)           |     1/46 (2%)       0/39 (0%)       0/21 (0%)      |     1/42 (2%)       1/1 (100%)      1/44 (2%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         ---             ---           |      729 (T)         729 (T)         640           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.364N        P=0.533N        P=0.656N       |     P=0.407         P=0.016 *       P=0.506        |
|INCIDENTAL TUMOR       |     P=0.364N        P=0.533N        P=0.656N       |     P=0.374         P=0.016 *       P=0.464        |
|LOGISTIC REGRESSION    |     P=0.364N        P=0.533N        P=0.656N       |     P=0.373         P=0.016 *       P=0.480        |
|COCHRAN-ARMITAGE       |     P=0.277N                                       |     P=0.399                                        |
|FISHER EXACT           |                     P=0.508N        P=0.513N       |                     P=0.033 *       P=0.500        |
|=================================================================================================================================|
|                                                                                                                                 |
|Thyroid Gland: Follicular Cell                                                                                                   |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     2/60 (3%)       0/58 (0%)       0/57 (0%)      |     1/60 (2%)       1/1 (100%)      2/60 (3%)      |
|ADJUSTED (b)           |      4.3%            0.0%            0.0%          |      2.4%          100.0%            4.2%          |
|TERMINAL (d)           |     2/46 (4%)       0/39 (0%)       0/21 (0%)      |     1/42 (2%)       1/1 (100%)      1/44 (2%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         ---             ---           |      729 (T)         729 (T)         640           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.168N        P=0.276N        P=0.423N       |     P=0.407         P=0.016 *       P=0.506        |
|INCIDENTAL TUMOR       |     P=0.168N        P=0.276N        P=0.423N       |     P=0.374         P=0.016 *       P=0.464        |
|LOGISTIC REGRESSION    |     P=0.168N        P=0.276N        P=0.423N       |     P=0.373         P=0.016 *       P=0.480        |
|COCHRAN-ARMITAGE       |     P=0.101N                                       |     P=0.399                                        |
|FISHER EXACT           |                     P=0.256N        P=0.261N       |                     P=0.033 *       P=0.500        |
|=================================================================================================================================|
 
         DATE: 09/18/94                     EXPERIMENT: 05705 TEST: 01                                                 PAGE  10
                            STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      CONTROL         50 MG/KG        100MG/KG      |      CONTROL         50 MG/KG        100MG/KG      |
|                       |      MALE            MALE            MALE          |      FEMALE          FEMALE          FEMALE        |
|=================================================================================================================================|
|                                                                                                                                 |
|Uterus                                                                                                                           |
|     Carcinoma                                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |     0/60 (0%)       0/60 (0%)       2/60 (3%)      |
|ADJUSTED (b)           |                                                    |      0.0%            0.0%            4.0%          |
|TERMINAL (d)           |                                                    |     0/42 (0%)       0/40 (0%)       1/44 (2%)      |
|FIRST INCIDENCE (DAYS) |                                                    |      ---             ---             100           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |     P=0.099           (e)           P=0.241        |
|INCIDENTAL TUMOR       |                                                    |     P=0.209           (e)           P=0.433        |
|LOGISTIC REGRESSION    |                                                    |     P=0.190           (e)           P=0.391        |
|COCHRAN-ARMITAGE       |                                                    |     P=0.096                                        |
|FISHER EXACT           |                                                    |                       (e)           P=0.248        |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangioma                                                                                                                  |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     3/60 (5%)       1/60 (2%)       0/60 (0%)      |     0/60 (0%)       0/60 (0%)       1/60 (2%)      |
|ADJUSTED (b)           |      6.5%            2.1%            0.0%          |      0.0%            0.0%            2.3%          |
|TERMINAL (d)           |     3/46 (7%)       0/39 (0%)       0/21 (0%)      |     0/42 (0%)       0/40 (0%)       1/44 (2%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         639             ---           |      ---             ---             729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.146N        P=0.361N        P=0.289N       |     P=0.279           (e)           P=0.509        |
|INCIDENTAL TUMOR       |     P=0.104N        P=0.331N        P=0.289N       |     P=0.279           (e)           P=0.509        |
|LOGISTIC REGRESSION    |     P=0.106N        P=0.337N        P=0.289N       |     P=0.279           (e)           P=0.509        |
|COCHRAN-ARMITAGE       |     P=0.061N                                       |     P=0.270                                        |
|FISHER EXACT           |                     P=0.309N        P=0.122N       |                       (e)           P=0.500        |
|=================================================================================================================================|
 
         DATE: 09/18/94                     EXPERIMENT: 05705 TEST: 01                                                 PAGE  11
                            STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      CONTROL         50 MG/KG        100MG/KG      |      CONTROL         50 MG/KG        100MG/KG      |
|                       |      MALE            MALE            MALE          |      FEMALE          FEMALE          FEMALE        |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangiosarcoma or Hemangioma                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     4/60 (7%)       2/60 (3%)       1/60 (2%)      |     1/60 (2%)       0/60 (0%)       1/60 (2%)      |
|ADJUSTED (b)           |      8.7%            4.6%            4.8%          |      1.7%            0.0%            2.3%          |
|TERMINAL (d)           |     4/46 (9%)       1/39 (3%)       1/21 (5%)      |     0/42 (0%)       0/40 (0%)       1/44 (2%)      |
|FIRST INCIDENCE (DAYS) |      729 (T)         639             729 (T)       |      511             ---             729 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.326N        P=0.411N        P=0.473N       |     P=0.661         P=0.507N        P=0.754        |
|INCIDENTAL TUMOR       |     P=0.270N        P=0.385N        P=0.473N       |     P=0.536         P=0.443N        P=0.509        |
|LOGISTIC REGRESSION    |     P=0.265N        P=0.384N        P=0.473N       |     P=0.638N        P=0.488N        P=0.744N       |
|COCHRAN-ARMITAGE       |     P=0.119N                                       |     P=0.668N                                       |
|FISHER EXACT           |                     P=0.340N        P=0.182N       |                     P=0.500N        P=0.752N       |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Histiocytic Sarcoma                                                                                                         |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/60 (0%)       0/60 (0%)       0/60 (0%)      |     3/60 (5%)       2/60 (3%)       3/60 (5%)      |
|ADJUSTED (b)           |      0.0%            0.0%            0.0%          |      6.2%            4.3%            6.3%          |
|TERMINAL (d)           |     0/46 (0%)       0/39 (0%)       0/21 (0%)      |     1/42 (2%)       0/40 (0%)       1/44 (2%)      |
|FIRST INCIDENCE (DAYS) |      ---             ---             ---           |      513             590             664           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |       (e)             (e)             (e)          |     P=0.575         P=0.531N        P=0.651        |
|INCIDENTAL TUMOR       |       (e)             (e)             (e)          |     P=0.352         P=0.475N        P=0.345        |
|LOGISTIC REGRESSION    |       (e)             (e)             (e)          |     P=0.578N        P=0.477N        P=0.665        |
|COCHRAN-ARMITAGE       |       (e)                                          |     P=0.588N                                       |
|FISHER EXACT           |                       (e)             (e)          |                     P=0.500N        P=0.660N       |
|=================================================================================================================================|
 
         DATE: 09/18/94                     EXPERIMENT: 05705 TEST: 01                                                 PAGE  12
                            STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      CONTROL         50 MG/KG        100MG/KG      |      CONTROL         50 MG/KG        100MG/KG      |
|                       |      MALE            MALE            MALE          |      FEMALE          FEMALE          FEMALE        |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Lymphoma And Histiocytic Sarcoma                                                                                  |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     8/60 (13%)      7/60 (12%)      5/60 (8%)      |    32/60 (53%)     20/60 (33%)     15/60 (25%)     |
|ADJUSTED (b)           |     16.1%           16.0%           21.3%          |     59.0%           38.0%           30.3%          |
|TERMINAL (d)           |     5/46 (11%)      4/39 (10%)      3/21 (14%)     |    20/42 (48%)      9/40 (23%)     10/44 (23%)     |
|FIRST INCIDENCE (DAYS) |      637             573             679           |      513             390             640           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.388         P=0.598         P=0.420        |     P=0.003N**      P=0.076N        P=0.003N**     |
|INCIDENTAL TUMOR       |     P=0.466N        P=0.483N        P=0.553N       |     P=0.010N*       P=0.019N*       P=0.011N*      |
|LOGISTIC REGRESSION    |     P=0.546N        P=0.560N        P=0.581        |     P=0.007N**      P=0.021N*       P=0.007N**     |
|COCHRAN-ARMITAGE       |     P=0.234N                                       |     P<0.001N**                                     |
|FISHER EXACT           |                     P=0.500N        P=0.279N       |                     P=0.021N*       P=0.001N**     |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Lymphoma: Histiocytic, Lymphocytic, Mixed,                                                                        |
|     NOS, or Undifferentiated Cell Type                                                                                          |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     8/60 (13%)      7/60 (12%)      5/60 (8%)      |    29/60 (48%)     18/60 (30%)     13/60 (22%)     |
|ADJUSTED (b)           |     16.1%           16.0%           21.3%          |     55.4%           35.2%           27.3%          |
|TERMINAL (d)           |     5/46 (11%)      4/39 (10%)      3/21 (14%)     |    19/42 (45%)      9/40 (23%)     10/44 (23%)     |
|FIRST INCIDENCE (DAYS) |      637             573             679           |      625             390             640           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.388         P=0.598         P=0.420        |     P=0.003N**      P=0.085N        P=0.003N**     |
|INCIDENTAL TUMOR       |     P=0.466N        P=0.483N        P=0.553N       |     P=0.009N**      P=0.033N*       P=0.008N**     |
|LOGISTIC REGRESSION    |     P=0.546N        P=0.560N        P=0.581        |     P=0.003N**      P=0.033N*       P=0.004N**     |
|COCHRAN-ARMITAGE       |     P=0.234N                                       |     P=0.001N**                                     |
|FISHER EXACT           |                     P=0.500N        P=0.279N       |                     P=0.030N*       P=0.002N**     |
|=================================================================================================================================|
 
         DATE: 09/18/94                     EXPERIMENT: 05705 TEST: 01                                                 PAGE  13
                            STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      CONTROL         50 MG/KG        100MG/KG      |      CONTROL         50 MG/KG        100MG/KG      |
|                       |      MALE            MALE            MALE          |      FEMALE          FEMALE          FEMALE        |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Benign Tumors                                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    20/60 (33%)     21/60 (35%)     11/60 (18%)     |    14/60 (23%)     11/60 (18%)     13/60 (22%)     |
|ADJUSTED (b)           |     42.5%           49.6%           40.7%          |     32.6%           25.7%           28.1%          |
|TERMINAL (d)           |    19/46 (41%)     18/39 (46%)      6/21 (29%)     |    13/42 (31%)      9/40 (23%)     11/44 (25%)     |
|FIRST INCIDENCE (DAYS) |      702             639             467           |      724             636             640           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.298         P=0.256         P=0.399        |     P=0.412N        P=0.381N        P=0.448N       |
|INCIDENTAL TUMOR       |     P=0.531N        P=0.311         P=0.499N       |     P=0.478N        P=0.367N        P=0.520N       |
|LOGISTIC REGRESSION    |     P=0.534         P=0.302         P=0.539N       |     P=0.504N        P=0.424N        P=0.533N       |
|COCHRAN-ARMITAGE       |     P=0.043N*                                      |     P=0.455N                                       |
|FISHER EXACT           |                     P=0.500         P=0.047N*      |                     P=0.327N        P=0.500N       |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Tumors                                                                                                            |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    21/60 (35%)     14/60 (23%)     13/60 (22%)     |    36/60 (60%)     27/60 (45%)     20/60 (33%)     |
|ADJUSTED (b)           |     39.3%           29.3%           44.5%          |     64.2%           47.4%           38.9%          |
|TERMINAL (d)           |    14/46 (30%)      6/39 (15%)      6/21 (29%)     |    22/42 (52%)     11/40 (28%)     13/44 (30%)     |
|FIRST INCIDENCE (DAYS) |      517             573             495           |      511             253             100           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.352         P=0.261N        P=0.296        |     P=0.010N*       P=0.200N        P=0.008N**     |
|INCIDENTAL TUMOR       |     P=0.137N        P=0.085N        P=0.255N       |     P=0.021N*       P=0.054N        P=0.026N*      |
|LOGISTIC REGRESSION    |     P=0.179N        P=0.081N        P=0.289N       |     P=0.015N*       P=0.024N*       P=0.006N**     |
|COCHRAN-ARMITAGE       |     P=0.061N                                       |     P=0.002N**                                     |
|FISHER EXACT           |                     P=0.114N        P=0.078N       |                     P=0.072N        P=0.003N**     |
|=================================================================================================================================|
 
         DATE: 09/18/94                     EXPERIMENT: 05705 TEST: 01                                                 PAGE  14
                            STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- MONOCHLOROACETIC ACID
 
                                                 TERMINAL SACRIFICE AT 105 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      CONTROL         50 MG/KG        100MG/KG      |      CONTROL         50 MG/KG        100MG/KG      |
|                       |      MALE            MALE            MALE          |      FEMALE          FEMALE          FEMALE        |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant and Benign Tumors                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    32/60 (53%)     32/60 (53%)     21/60 (35%)     |    41/60 (68%)     33/60 (55%)     27/60 (45%)     |
|ADJUSTED (b)           |     60.2%           65.2%           66.6%          |     73.2%           58.3%           52.7%          |
|TERMINAL (d)           |    25/46 (54%)     22/39 (56%)     11/21 (52%)     |    27/42 (64%)     17/40 (43%)     20/44 (45%)     |
|FIRST INCIDENCE (DAYS) |      517             573             467           |      511             253             100           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.096         P=0.269         P=0.121        |     P=0.021N*       P=0.255N        P=0.017N*      |
|INCIDENTAL TUMOR       |     P=0.404N        P=0.454         P=0.418N       |     P=0.052N        P=0.090N        P=0.058N       |
|LOGISTIC REGRESSION    |     P=0.441N        P=0.367         P=0.437N       |     P=0.039N*       P=0.049N*       P=0.021N*      |
|COCHRAN-ARMITAGE       |     P=0.027N*                                      |     P=0.007N**                                     |
|FISHER EXACT           |                     P=0.573N        P=0.033N*      |                     P=0.094N        P=0.008N**     |
|=================================================================================================================================|
 
 (a)  Number of tumor-bearing animals / number of animals examined at site.
 (b)  Kaplan-Meier estimated lifetime tumor incidence after adjustment for 
      intercurrent mortality.
 (d)  Observed incidence at terminal kill.
 (f)  Beneath the control incidence are the P-values associated with the trend 
      test.  Beneath the dosed group incidence are the P-values corresponding to 
      pairwise comparisons between the controls and that dosed group.  The life
      table analysis regards tumors in animals dying  prior to terminal kill as
      being (directly or indirectly) the cause of death.  The incidental tumor 
      test regards these lesions as non-fatal.  Logistic regression is an alternative
      method for analyzing the incidence of non-fatal tumors.  The Cochran-Armitage
      and Fisher's exact tests compare directly the overall incidence rates.  
      For all tests, a negative trend is indicated by "N".
 (e)  Value of Statistic cannot be computed. 
 (I)  Interim sacrifice
 (T)  Terminal sacrifice
  #   Tumor rates based on number of animals necropsied.
 
  *   To the right of any statistical result, indicates significance at (P<=0.05).
  **  To the right of any statistical result, indicates significance at (P<=0.01).
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.