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TDMS Study 91005-07 Pathology Tables

NTP Experiment-Test: 91005-07  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05
Route: GAVAGE                                                                                                     Time: 10:08:31

                                                         FINAL #1 RATS




       Facility:  Southern Research Institute

       Chemical CAS #:  7220-79-3

       Lock Date:  09/05/03

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All


































a  Number of animals examined microscopically at site and number of animals with lesion

                                                              Page   1


NTP Experiment-Test: 91005-07  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05  
Route: GAVAGE                                                                                                     Time: 10:08:31  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                   0 MG/KG      5 MG/KG      25 MG/KG     50 MG/KG                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially in Study                          50           50           50           50                                     
  Early Deaths                                                                                                                      
    Natural Death                                      6            4            9           11                                     
    Moribund Sacrifice                                 9           13            5            3                                     
    Dosing Accident                                                                           1                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                35           32           34           35                                     
    Natural Death                                                                2                                                  
  Other                                                             1                                                               
                                                                                                                                    
  Animals Examined Microscopically                    50           49           50           50                                     
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Liver                                              (50)         (49)         (50)         (50)                                   
      Granulosa Cell Tumor Malignant, Metastatic,                                                                                   
           Ovary                                                                 1 (2%)                                             
   Mesentery                                          (17)         (14)         (12)         (8)                                    
      Granulosa Cell Tumor Malignant, Metastatic,                                                                                   
           Ovary                                                                 1 (8%)                                             
   Oral Mucosa                                        (2)                                    (1)                                    
      Pharyngeal, Squamous Cell Carcinoma              1 (50%)                                                                      
   Pancreas                                           (49)         (48)         (48)         (49)                                   
      Acinus, Adenoma                                  1 (2%)                                                                       
   Salivary Glands                                    (50)         (49)         (50)         (50)                                   
      Schwannoma Malignant                                                       1 (2%)                                             
   Stomach, Forestomach                               (50)         (49)         (49)         (49)                                   
      Squamous Cell Papilloma                          1 (2%)                                                                       
   Tongue                                             (1)          (1)          (1)          (1)                                    
      Squamous Cell Papilloma                                                    1 (100%)                                           
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (50)         (49)         (50)         (50)                                   
      Schwannoma Benign                                                          1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
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NTP Experiment-Test: 91005-07  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05  
Route: GAVAGE                                                                                                     Time: 10:08:31  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                   0 MG/KG      5 MG/KG      25 MG/KG     50 MG/KG                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (50)         (49)         (50)         (50)                                   
      Adenoma                                          1 (2%)                                                                       
   Adrenal Medulla                                    (50)         (49)         (50)         (50)                                   
      Pheochromocytoma Complex                                      1 (2%)                                                          
      Pheochromocytoma Benign                          2 (4%)                    1 (2%)       1 (2%)                                
   Islets, Pancreatic                                 (49)         (48)         (48)         (49)                                   
      Adenoma                                          2 (4%)                    1 (2%)       1 (2%)                                
   Pituitary Gland                                    (50)         (49)         (50)         (50)                                   
      Pars Distalis, Adenoma                          36 (72%)     32 (65%)     31 (62%)     28 (56%)                               
   Thyroid Gland                                      (50)         (49)         (50)         (50)                                   
      Bilateral, C-Cell, Adenoma                       1 (2%)                                 2 (4%)                                
      C-Cell, Adenoma                                  6 (12%)      5 (10%)      8 (16%)      1 (2%)                                
      C-Cell, Carcinoma                                             1 (2%)                                                          
      Follicular Cell, Adenoma                                                   1 (2%)                                             
      Follicular Cell, Carcinoma                                    1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Clitoral Gland                                     (50)         (48)         (50)         (50)                                   
      Adenoma                                          7 (14%)     10 (21%)      8 (16%)      9 (18%)                               
      Carcinoma                                        1 (2%)       1 (2%)       1 (2%)       1 (2%)                                
   Ovary                                              (50)         (49)         (50)         (50)                                   
      Granulosa Cell Tumor Malignant                                             1 (2%)                                             
   Uterus                                             (50)         (49)         (50)         (50)                                   
      Adenoma                                                                    1 (2%)                                             
      Polyp Stromal                                   13 (26%)     14 (29%)     13 (26%)      7 (14%)                               
      Sarcoma Stromal                                  2 (4%)                    1 (2%)       1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (50)         (49)         (50)         (50)                                   
      Histiocytic Sarcoma                              1 (2%)                                                                       
   Lymph Node                                         (15)         (8)          (11)         (3)                                    
   Lymph Node, Mesenteric                             (49)         (49)         (50)         (50)                                   
   Spleen                                             (49)         (48)         (49)         (49)                                   
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   3                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05  
Route: GAVAGE                                                                                                     Time: 10:08:31  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                   0 MG/KG      5 MG/KG      25 MG/KG     50 MG/KG                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - cont                                                                                                         
      Granulosa Cell Tumor Malignant, Metastatic,                                                                                   
           Ovary                                                                 1 (2%)                                             
      Histiocytic Sarcoma                                           1 (2%)                                                          
   Thymus                                             (50)         (49)         (50)         (50)                                   
      Thymoma Benign                                   1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (50)         (49)         (50)         (50)                                   
      Adenoma                                          1 (2%)       2 (4%)                                                          
      Carcinoma                                        1 (2%)                                                                       
      Fibroadenoma                                    20 (40%)     17 (35%)     22 (44%)     13 (26%)                               
      Fibroadenoma, Multiple                           8 (16%)     13 (27%)      6 (12%)      4 (8%)                                
   Skin                                               (50)         (49)         (50)         (50)                                   
      Basal Cell Carcinoma                             1 (2%)                                                                       
      Squamous Cell Papilloma                          1 (2%)       1 (2%)                                                          
      Trichoepithelioma                                1 (2%)                    1 (2%)                                             
      Pinna, Neural Crest Tumor                                                               1 (2%)                                
      Subcutaneous Tissue, Fibroma                     1 (2%)       1 (2%)       2 (4%)                                             
      Subcutaneous Tissue, Fibrosarcoma                1 (2%)       3 (6%)                                                          
      Subcutaneous Tissue, Hemangiosarcoma             1 (2%)                                                                       
      Subcutaneous Tissue, Lipoma                                   1 (2%)                    1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (50)         (49)         (50)         (50)                                   
      Osteosarcoma                                     1 (2%)                                                                       
      Vertebra, Chordoma                                                                      1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (50)         (49)         (50)         (50)                                   
      Astrocytoma Malignant                                                      1 (2%)                                             
   Peripheral Nerve                                   (3)          (4)          (4)          (2)                                    
      Schwannoma Malignant                                          1 (25%)                                                         
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   4                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05  
Route: GAVAGE                                                                                                     Time: 10:08:31  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                   0 MG/KG      5 MG/KG      25 MG/KG     50 MG/KG                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (49)         (50)         (50)                                   
      Alveolar/Bronchiolar Adenoma                                  1 (2%)       1 (2%)                                             
      Chordoma, Metastatic, Bone                                                              1 (2%)                                
      Pheochromocytoma Complex, Metastatic,                                                                                         
          Adrenal Medulla                                           1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                (50)         (49)         (50)         (50)                                   
   Harderian Gland                                    (50)         (49)         (50)         (50)                                   
   Zymbal's Gland                                                  (1)          (1)                                                 
      Carcinoma                                                     1 (100%)     1 (100%)                                           
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (50)         (49)         (50)         (50)                                   
      Renal Tubule, Adenoma                                                                   1 (2%)                                
   Urinary Bladder                                    (50)         (49)         (50)         (50)                                   
      Transitional Epithelium, Papilloma                            1 (2%)                                                          
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
                                                                                                                                    
   Multiple Organs                                   *(50)        *(49)        *(50)        *(50)                                   
      Histiocytic Sarcoma                              1 (2%)       1 (2%)                                                          
      Leukemia Mononuclear                            12 (24%)      6 (12%)      3 (6%)       2 (4%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   5                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05  
Route: GAVAGE                                                                                                     Time: 10:08:31  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                   0 MG/KG      5 MG/KG      25 MG/KG     50 MG/KG                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)            50          48          46          40                                       
     Total Primary Neoplasms                          125         114         107          74                                       
                                                                                                                                    
   Total Animals with Benign Neoplasms                 47          45          45          40                                       
     Total Benign Neoplasms                           103          98          98          68                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms              19          16           8           5                                       
     Total Malignant Neoplasms                         22          16           9           5                                       
                                                                                                                                    
   Total Animals with Metastatic Neoplasms                          1           1           1                                       
     Total Metastatic Neoplasm                                      1           3           1                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms                                                                                           
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                                                      1                                       
     Total Uncertain Neoplasms                                                              1                                       
                                                                                                                                    
                                                                                                                                    
____________________________________________________________________________________________________________________________________
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   6                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05  
Route: GAVAGE                                                                                                     Time: 10:08:31  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS MALE                     0 MG/KG      5 MG/KG      25 MG/KG     50 MG/KG                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially in Study                          50           50           50           50                                     
  Early Deaths                                                                                                                      
    Natural Death                                      6           10            5            7                                     
    Moribund Sacrifice                                13            7            5           10                                     
    Dosing Accident                                                              1            2                                     
  Survivors                                                                                                                         
    Natural Death                                      1                                                                            
    Terminal Sacrifice                                29           33           39           30                                     
    Moribund Sacrifice                                 1                                      1                                     
                                                                                                                                    
  Animals Examined Microscopically                    50           50           50           50                                     
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Intestine Small, Duodenum                          (50)         (50)         (50)         (48)                                   
      Schwannoma Malignant                                          1 (2%)                                                          
   Intestine Small, Jejunum                           (47)         (47)         (47)         (48)                                   
   Intestine Small, Ileum                             (48)         (50)         (49)         (48)                                   
   Liver                                              (50)         (50)         (50)         (50)                                   
      Hemangiosarcoma                                  1 (2%)                                                                       
      Hepatocellular Carcinoma                                                   1 (2%)                                             
      Hepatocellular Adenoma                           1 (2%)       1 (2%)                                                          
   Mesentery                                          (16)         (11)         (9)          (12)                                   
      Carcinoma, Metastatic, Zymbal's Gland                                                   1 (8%)                                
      Fibrous Histiocytoma, Metastatic, Skin                                                  1 (8%)                                
      Hemangiosarcoma                                  1 (6%)                                                                       
   Oral Mucosa                                        (1)                                    (4)                                    
      Pharyngeal, Squamous Cell Carcinoma              1 (100%)                               1 (25%)                               
   Pancreas                                           (50)         (50)         (50)         (50)                                   
      Carcinoma, Metastatic, Zymbal's Gland                                                   1 (2%)                                
      Mixed Tumor Benign                               1 (2%)       1 (2%)                    1 (2%)                                
      Acinus, Adenoma                                               2 (4%)       4 (8%)       3 (6%)                                
   Stomach, Forestomach                               (50)         (50)         (50)         (50)                                   
   Stomach, Glandular                                 (50)         (50)         (50)         (50)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   7                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05  
Route: GAVAGE                                                                                                     Time: 10:08:31  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS MALE                     0 MG/KG      5 MG/KG      25 MG/KG     50 MG/KG                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (50)         (50)         (50)         (50)                                   
      Carcinoma, Metastatic, Zymbal's Gland                                                   1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (50)         (50)         (50)         (50)                                   
      Adenoma                                          1 (2%)                    1 (2%)       2 (4%)                                
      Osteosarcoma, Metastatic, Nose                                1 (2%)                                                          
   Adrenal Medulla                                    (50)         (50)         (50)         (50)                                   
      Pheochromocytoma Malignant                       1 (2%)                                 1 (2%)                                
      Pheochromocytoma Complex                         1 (2%)                                                                       
      Pheochromocytoma Benign                          7 (14%)     12 (24%)      2 (4%)       2 (4%)                                
      Bilateral, Pheochromocytoma Benign               2 (4%)       1 (2%)                    1 (2%)                                
   Islets, Pancreatic                                 (50)         (50)         (50)         (50)                                   
      Adenoma                                          4 (8%)       8 (16%)     12 (24%)      7 (14%)                               
      Adenoma, Multiple                                             1 (2%)                    1 (2%)                                
      Carcinoma                                                                  2 (4%)                                             
   Pituitary Gland                                    (50)         (50)         (50)         (50)                                   
      Pars Distalis, Adenoma                          25 (50%)     32 (64%)     26 (52%)     21 (42%)                               
      Pars Distalis, Carcinoma                                                   1 (2%)                                             
   Thyroid Gland                                      (50)         (50)         (50)         (50)                                   
      Bilateral, C-Cell, Adenoma                       2 (4%)                    1 (2%)                                             
      C-Cell, Adenoma                                  4 (8%)       3 (6%)       6 (12%)      4 (8%)                                
      C-Cell, Carcinoma                                2 (4%)       2 (4%)                                                          
      Follicular Cell, Adenoma                                                   2 (4%)       2 (4%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   Peritoneum                                         (1)          (1)          (1)          (2)                                    
      Carcinoma, Metastatic, Zymbal's Gland                                                   1 (50%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Epididymis                                         (50)         (50)         (50)         (50)                                   
   Preputial Gland                                    (50)         (50)         (50)         (50)                                   
      Adenoma                                          1 (2%)       4 (8%)       5 (10%)                                            
      Bilateral, Adenoma                                                         1 (2%)                                             
   Prostate                                           (50)         (50)         (50)         (50)                                   
      Adenoma                                          1 (2%)                                                                       
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   8                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05  
Route: GAVAGE                                                                                                     Time: 10:08:31  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS MALE                     0 MG/KG      5 MG/KG      25 MG/KG     50 MG/KG                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
GENITAL SYSTEM - cont                                                                                                               
      Carcinoma, Metastatic, Zymbal's Gland                                                   1 (2%)                                
   Seminal Vesicle                                    (50)         (50)         (50)         (50)                                   
   Testes                                             (50)         (50)         (50)         (50)                                   
      Bilateral, Interstitial Cell, Adenoma           32 (64%)     28 (56%)     31 (62%)     31 (62%)                               
      Interstitial Cell, Adenoma                       9 (18%)     11 (22%)     14 (28%)      3 (6%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (50)         (50)         (50)         (50)                                   
   Lymph Node                                         (21)         (17)         (10)         (18)                                   
      Mediastinal, Carcinoma, Metastatic,                                                                                           
          Harderian Gland                                                                     1 (6%)                                
      Pancreatic, Carcinoma, Metastatic, Zymbal's                                                                                   
          Gland                                                                               1 (6%)                                
   Lymph Node, Mesenteric                             (50)         (50)         (50)         (50)                                   
      Carcinoma, Metastatic, Zymbal's Gland                                                   1 (2%)                                
   Spleen                                             (50)         (50)         (50)         (50)                                   
      Carcinoma, Metastatic, Zymbal's Gland                                                   1 (2%)                                
      Hemangiosarcoma                                  1 (2%)       1 (2%)       1 (2%)                                             
   Thymus                                             (49)         (50)         (50)         (50)                                   
      Carcinoma, Metastatic, Zymbal's Gland                                                   1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (50)         (50)         (50)         (50)                                   
      Fibroadenoma                                     2 (4%)       3 (6%)       1 (2%)       1 (2%)                                
   Skin                                               (50)         (50)         (50)         (50)                                   
      Basal Cell Adenoma                                            2 (4%)       1 (2%)                                             
      Keratoacanthoma                                  3 (6%)       1 (2%)       3 (6%)                                             
      Squamous Cell Carcinoma                                                    2 (4%)                                             
      Squamous Cell Papilloma                          3 (6%)       2 (4%)       5 (10%)                                            
      Squamous Cell Papilloma, Multiple                1 (2%)                                 1 (2%)                                
      Lip, Fibrosarcoma                                1 (2%)                                                                       
      Pinna, Neural Crest Tumor                        1 (2%)                                                                       
      Sebaceous Gland, Adenoma                                                   1 (2%)                                             
      Subcutaneous Tissue, Fibroma                     5 (10%)      4 (8%)       1 (2%)       1 (2%)                                
      Subcutaneous Tissue, Fibroma, Multiple           1 (2%)                                                                       
      Subcutaneous Tissue, Fibrosarcoma                2 (4%)       2 (4%)                                                          
      Subcutaneous Tissue, Fibrous Histiocytoma                                               1 (2%)                                
      Subcutaneous Tissue, Hemangiosarcoma                                       1 (2%)                                             
      Subcutaneous Tissue, Lipoma                                                             1 (2%)                                
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   9                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05  
Route: GAVAGE                                                                                                     Time: 10:08:31  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS MALE                     0 MG/KG      5 MG/KG      25 MG/KG     50 MG/KG                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (50)         (50)         (50)         (50)                                   
      Osteosarcoma                                                               1 (2%)       1 (2%)                                
   Skeletal Muscle                                    (3)          (1)                       (4)                                    
      Carcinoma, Metastatic, Zymbal's Gland                                                   1 (25%)                               
      Hemangioma                                       1 (33%)                                                                      
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 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (50)         (50)         (50)         (50)                                   
      Carcinoma, Metastatic, Pituitary Gland                                     1 (2%)                                             
      Glioma Malignant                                              1 (2%)                                                          
   Peripheral Nerve                                   (6)          (3)          (4)          (6)                                    
      Schwannoma Benign                                             1 (33%)                                                         
      Schwannoma Malignant                             1 (17%)                   1 (25%)                                            
      Trigeminal, Squamous Cell Carcinoma,                                                                                          
          Metastatic, Oral Mucosa                      1 (17%)                                                                      
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 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Adenoma                     1 (2%)                    1 (2%)       2 (4%)                                
      Alveolar/Bronchiolar Carcinoma                                                          2 (4%)                                
      Carcinoma, Metastatic, Thyroid Gland                          1 (2%)                                                          
      Carcinoma, Metastatic, Zymbal's Gland                                                   1 (2%)                                
      Fibrous Histiocytoma, Metastatic, Skin                                                  1 (2%)                                
      Osteosarcoma, Metastatic, Nose                                1 (2%)                                                          
      Pheochromocytoma Malignant, Metastatic,                                                                                       
          Adrenal Medulla                              1 (2%)                                                                       
      Schwannoma Malignant, Metastatic, Peripheral                                                                                  
          Nerve                                        1 (2%)                                                                       
      Squamous Cell Carcinoma, Metastatic, Skin                                  1 (2%)                                             
   Nose                                               (50)         (50)         (50)         (50)                                   
      Carcinoma, Metastatic, Harderian Gland                                                  1 (2%)                                
      Osteosarcoma                                                  1 (2%)                                                          
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                                                             Page  10                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05  
Route: GAVAGE                                                                                                     Time: 10:08:31  
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         FISCHER 344 RATS MALE                     0 MG/KG      5 MG/KG      25 MG/KG     50 MG/KG                                  
                                                                                                                                    
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 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Harderian Gland                                    (50)         (50)         (50)         (50)                                   
      Adenoma                                                                    1 (2%)                                             
      Carcinoma                                                                               1 (2%)                                
   Zymbal's Gland                                                  (1)          (1)          (1)                                    
      Adenoma                                                                    1 (100%)                                           
      Carcinoma                                                     1 (100%)                  1 (100%)                              
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 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (50)         (50)         (50)         (50)                                   
      Carcinoma, Metastatic, Zymbal's Gland                                                   1 (2%)                                
      Renal Tubule, Adenoma                                                                   1 (2%)                                
   Urinary Bladder                                    (50)         (50)         (50)         (49)                                   
      Papilloma                                                                  1 (2%)                                             
                                                                                                                                    
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 SYSTEMIC LESIONS                                                                                                                   
                                                                                                                                    
   Multiple Organs                                   *(50)        *(50)        *(50)        *(50)                                   
      Leukemia Mononuclear                            23 (46%)     10 (20%)      2 (4%)       2 (4%)                                
      Mesothelioma Malignant                           3 (6%)       5 (10%)      2 (4%)       3 (6%)                                
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     *   Number of animals with any tissue examined microscopically                                                                 
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  11                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-07  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 03/25/05  
Route: GAVAGE                                                                                                     Time: 10:08:31  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS MALE                     0 MG/KG      5 MG/KG      25 MG/KG     50 MG/KG                                  
                                                                                                                                    
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TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)            49          50          50          48                                       
     Total Primary Neoplasms                          146         141         135          98                                       
                                                                                                                                    
   Total Animals with Benign Neoplasms                 47          50          50          48                                       
     Total Benign Neoplasms                           107         117         121          85                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms              30          19          12          12                                       
     Total Malignant Neoplasms                         38          24          14          13                                       
                                                                                                                                    
   Total Animals with Metastatic Neoplasms              3           2           2           3                                       
     Total Metastatic Neoplasm                          3           3           2          16                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms                                                                                           
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                  1                                                                           
     Total Uncertain Neoplasms                          1                                                                           
                                                                                                                                    
                                                                                                                                    
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a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
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