Skip to Main Navigation
Skip to Page Content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
https://ntp.niehs.nih.gov/go/20989

TDMS Study 91005-08 Pathology Tables

NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05
Route: GAVAGE                                                                                                     Time: 13:15:34

                                                   FINAL #1; REVISION #1 MICE




       Facility:  Southern Research Institute

       Chemical CAS #:  7220-79-3

       Lock Date:  09/05/03

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All




































                                                              Page   1


NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 5| 7| 7| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 5| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 8| 0| 3| 3| 3| 3| 1| 3| 3| 3| 3| 2|             
                                           | 2| 2| 0| 2| 2| 8| 1| 7| 7| 7| 0| 6| 6| 5| 4| 6| 6| 6| 6| 6| 2| 2| 2| 2| 1|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Gallbladder                             | +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  A|             
      Cyst                                 |                      X                          X                        |             
                                           |__________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A|             
                                           |__________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A|             
                                           |__________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  A|             
      Edema                                |                                           3                              |             
                                           |__________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  A|             
                                           |__________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  A|             
                                           |__________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  A|             
                                           |__________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                                  2                                       |             
      Clear Cell Focus                     |             X                                                            |             
      Congestion                           |                                        3                                 |             
      Eosinophilic Focus                   |                                                                          |             
      Fibrosis, Focal                      |                                                                          |             
      Hematopoietic Cell Proliferation     |                                                                          |             
      Hemorrhage                           |       3                                                                  |             
      Hepatodiaphragmatic Nodule           |       2                                                                  |             
      Infiltration Cellular, Mixed Cell    |    1     2                    2              2              1  1  1      |             
      Mixed Cell Focus                     |       X                                                                  |             
      Bile Duct, Cyst                      |                                                                          |             
      Hepatocyte, Necrosis, Focal          |                                                                         2|             
      Hepatocyte, Vacuolization Cytoplasmic|       3                                   3              2        2      |             
      Kupffer Cell, Pigmentation           |                               1                                          |             
                                           |__________________________________________________________________________|             
   Mesentery                               | +                       +     +           +  +     +        +  +  +      |             
      Artery, Inflammation, Chronic, Focal |                               3                                          |             
      Fat, Necrosis, Focal                 | 3                       1     2           3  1     3        2  3  3      |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page   2                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 5| 7| 7| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 5| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 8| 0| 3| 3| 3| 3| 1| 3| 3| 3| 3| 2|             
                                           | 2| 2| 0| 2| 2| 8| 1| 7| 7| 7| 0| 6| 6| 5| 4| 6| 6| 6| 6| 6| 2| 2| 2| 2| 1|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM - cont                  |                                                                          |             
                                           |__________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A|             
      Acinus, Atrophy, Focal               |                            4                                             |             
      Duct, Cyst                           |                            X                                             |             
                                           |__________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Diverticulum                         |                                                                          |             
      Inflammation, Focal                  |                                                                          |             
      Ulcer                                |                                                                          |             
      Epithelium, Hyperplasia              |                                                                          |             
                                           |__________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A|             
      Cyst                                 |                                                                          |             
      Epithelium, Cytoplasmic Alteration,  |                                                                          |             
           Focal                           |                                                                   2      |             
      Epithelium, Hyperplasia              |                                           2                              |             
                                           |__________________________________________________________________________|             
   Tooth                                   |                                                       +                  |             
      Dysplasia                            |                                                       2                  |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       |                                                                          |             
      Thrombosis                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Accessory Adrenal Cortical Nodule    |                                  1           3                           |             
      Cytoplasmic Alteration, Focal        |                                                                          |             
      Degeneration, Cystic                 |                                                                          |             
      Infiltration Cellular, Mixed Cell    |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page   3                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 5| 7| 7| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 5| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 8| 0| 3| 3| 3| 3| 1| 3| 3| 3| 3| 2|             
                                           | 2| 2| 0| 2| 2| 8| 1| 7| 7| 7| 0| 6| 6| 5| 4| 6| 6| 6| 6| 6| 2| 2| 2| 2| 1|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A|             
                                           |__________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M|             
      Cyst                                 |                                                                          |             
                                           |__________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +|             
      Angiectasis                          |                                                                1         |             
      Pars Distalis, Cytoplasmic           |                                                                          |             
          Alteration, Focal                |                                                    1                     |             
      Pars Distalis, Hyperplasia, Focal    |                         1                                                |             
                                           |__________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I|             
      Cyst                                 |                                                    X                     |             
      Inflammation, Chronic, Focal         |                               2                                          |             
      Follicle, Degeneration, Cystic, Focal| 2           2           1        3                    2              2   |             
      Follicular Cell, Hyperplasia, Focal  |                                     1           2                        |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |__________________________________________________________________________|             
   Tissue NOS                              |                               +                                          |             
      Pelvic, Fibrosis                     |                               3                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  M  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M|             
      Angiectasis                          |    3                                4                                    |             
      Cyst                                 |    X              X                          X  X     X           X      |             
      Thrombosis                           | 4                 4                 4                                    |             
      Bilateral, Cyst                      |                               X                                          |             
                                           |__________________________________________________________________________|             
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis                          |                                                                   3      |             
      Inflammation, Suppurative            |                                                                          |             
      Endometrium, Hyperplasia, Cystic     | 3  3  3  3  3  3  2  3  3  3     3  1     3  3  3  3  3  3  3  3  3  3  2|             
                                           |__________________________________________________________________________|             
   Vagina                                  |                                                 +                        |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page   4                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 5| 7| 7| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 5| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 8| 0| 3| 3| 3| 3| 1| 3| 3| 3| 3| 2|             
                                           | 2| 2| 0| 2| 2| 8| 1| 7| 7| 7| 0| 6| 6| 5| 4| 6| 6| 6| 6| 6| 2| 2| 2| 2| 1|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                           2  4                    3      |             
                                           |__________________________________________________________________________|             
   Lymph Node                              |                                                                      +  +|             
      Iliac, Angiectasis                   |                                                                          |             
      Iliac, Hyperplasia, Lymphoid         |                                                                          |             
      Mediastinal, Hyperplasia, Lymphoid   |                                                                      3   |             
      Renal, Hyperplasia, Lymphoid         |                                                                         2|             
                                           |__________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I  +|             
      Hemorrhage                           |          3                                                               |             
      Hyperplasia, Lymphoid                |                         2  2                 2     2        2     2      |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |             3                                                            |             
      Hyperplasia, Lymphoid                |                                                       2     2            |             
                                           |__________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hematopoietic Cell Proliferation     |    2  2           2     2     3     3     3  3        3  3  3     3     3|             
      Hyperplasia, Lymphoid                |    3  2     2           2                    3     2              3  2   |             
                                           |__________________________________________________________________________|             
   Thymus                                  | +  +  I  +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  +  +  I  +  +  +  +|             
      Hyperplasia, Lymphoid                |                                              4     3           2     3   |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperostosis                         |                   1                             2     1        2         |             
      Vertebra, Hypertrophy, Focal         |                                                                          |             
                                           |__________________________________________________________________________|             
   Skeletal Muscle                         |                                                                          |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page   5                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 5| 7| 7| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 5| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 8| 0| 3| 3| 3| 3| 1| 3| 3| 3| 3| 2|             
                                           | 2| 2| 0| 2| 2| 8| 1| 7| 7| 7| 0| 6| 6| 5| 4| 6| 6| 6| 6| 6| 2| 2| 2| 2| 1|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Compression, Focal                   |                                                                          |             
      Artery, Meninges, Inflammation,      |                                                                          |             
          Chronic                          |                               2                                          |             
                                           |__________________________________________________________________________|             
   Peripheral Nerve                        |                               +                                          |             
                                           |__________________________________________________________________________|             
   Spinal Cord                             |                               +                                          |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                        3                                 |             
      Foreign Body                         |                                  3                                       |             
      Hemorrhage                           |                                        3                                 |             
      Hyperplasia, Histiocytic             |                                                                          |             
      Infiltration Cellular,               |                                                                          |             
          Polymorphonuclear                |                                                                          |             
      Infiltration Cellular, Mixed Cell    |                                                                          |             
      Inflammation                         |                                  2                                       |             
      Metaplasia, Focal, Osseous           |                                                                         1|             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |       1                                   2                              |             
                                           |__________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  A|             
      Anterior Chamber, Inflammation       |                                                                          |             
      Cornea, Inflammation                 |                                                                          |             
                                           |__________________________________________________________________________|             
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Epithelium, Hyperplasia              |                                                                          |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   6                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 5| 7| 7| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 5| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 8| 0| 3| 3| 3| 3| 1| 3| 3| 3| 3| 2|             
                                           | 2| 2| 0| 2| 2| 8| 1| 7| 7| 7| 0| 6| 6| 5| 4| 6| 6| 6| 6| 6| 2| 2| 2| 2| 1|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A|             
      Hyperplasia, Lymphoid                |                                                                          |             
      Metaplasia, Focal, Osseous           |                         3                                         1      |             
      Nephropathy                          |          1                    2                 1                    1   |             
      Renal Tubule, Accumulation, Hyaline  |                                                                          |             
          Droplet                          |                                           3                              |             
      Renal Tubule, Cyst                   |                                                    X                     |             
                                           |__________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                               2                                          |             
      Transitional Epithelium, Hyperplasia |                               2                                          |             
 __________________________________________|__________________________________________________________________________              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   7                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 6| 6| 7| 7| 6| 6| 7| 5| 7| 7| 7| 6| 7| 7| 6| 6| 7| 7| 6| 7|            |
                             DAY ON TEST   | 3| 3| 1| 3| 3| 3| 1| 3| 3| 5| 0| 3| 6| 3| 3| 3| 6| 3| 3| 7| 2| 3| 3| 6| 2|            |
                                           | 1| 7| 8| 7| 7| 8| 7| 7| 1| 5| 4| 6| 8| 6| 6| 7| 3| 7| 7| 3| 7| 6| 6| 6| 9|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Gallbladder                             | +  +  +  +  +  +  +  +  +  +  A  +  A  +  +  +  +  +  +  M  +  +  +  M  A|  42        |
      Cyst                                 |                                                                          |      2     |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +|  48        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +|  48        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  A  +  +  +  +  +  +  +  A  +  A  +  +  +  +  +  +  A  +  +  +  A  +|  43        |
      Edema                                |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  A  +  +  +  +  +  +  +  A  +  A  +  +  +  +  +  +  A  +  +  +  A  +|  43        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  A  +  +  +  +  +  +  +  A  +  A  +  +  +  +  +  +  A  +  +  +  A  +|  43        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  A  +  +  +  +  +  +  +  A  +  A  +  +  +  +  +  +  A  +  +  +  A  +|  43        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Angiectasis, Focal                   |                                                    2                     |      2  2.0|
      Clear Cell Focus                     |                                              X  X                        |      3     |
      Congestion                           |                                                                          |      1  3.0|
      Eosinophilic Focus                   |          X                                                               |      1     |
      Fibrosis, Focal                      |                                                                      1   |      1  1.0|
      Hematopoietic Cell Proliferation     |          1                    1  1                                   2   |      4  1.3|
      Hemorrhage                           |                                                                          |      1  3.0|
      Hepatodiaphragmatic Nodule           |                                                                          |      1  2.0|
      Infiltration Cellular, Mixed Cell    |                         2                 1        2        1            |     11  1.5|
      Mixed Cell Focus                     |          X                                                               |      2     |
      Bile Duct, Cyst                      |                            X                                             |      1     |
      Hepatocyte, Necrosis, Focal          |                                           1  2                           |      3  1.7|
      Hepatocyte, Vacuolization Cytoplasmic|          3     3  2        3  2              2  3                       4|     12  2.7|
      Kupffer Cell, Pigmentation           |                                                                          |      1  1.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page   8                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 6| 6| 7| 7| 6| 6| 7| 5| 7| 7| 7| 6| 7| 7| 6| 6| 7| 7| 6| 7|            |
                             DAY ON TEST   | 3| 3| 1| 3| 3| 3| 1| 3| 3| 5| 0| 3| 6| 3| 3| 3| 6| 3| 3| 7| 2| 3| 3| 6| 2|            |
                                           | 1| 7| 8| 7| 7| 8| 7| 7| 1| 5| 4| 6| 8| 6| 6| 7| 3| 7| 7| 3| 7| 6| 6| 6| 9|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mesentery                               | +     +           +  +  +  +           +        +                       +|  18        |
      Artery, Inflammation, Chronic, Focal |                                                                          |      1  3.0|
      Fat, Necrosis, Focal                 |                   3  3  3  3           1        1                       3|     16  2.4|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  A  +  +  +  +  A|  46        |
      Acinus, Atrophy, Focal               |                                                                          |      1  4.0|
      Duct, Cyst                           |                X                                                         |      2     |
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Diverticulum                         |       1                                                                  |      1  1.0|
      Inflammation, Focal                  |                                                 1                        |      1  1.0|
      Ulcer                                | 2              2                                                         |      2  2.0|
      Epithelium, Hyperplasia              | 3                                            1                           |      2  2.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  A  +  +  +  +  +  +  +  A  +  A  +  +  +  +  +  +  A  +  +  +  +  +|  45        |
      Cyst                                 |                                        X                                 |      1     |
      Epithelium, Cytoplasmic Alteration,  |                                                                          |            |
           Focal                           |                                                                          |      1  2.0|
      Epithelium, Hyperplasia              |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Tooth                                   |                                                                +         |   2        |
      Dysplasia                            |                                                                1         |      2  1.5|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cardiomyopathy                       |                                           1                              |      1  1.0|
      Thrombosis                           |                                                                      3   |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page   9                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 6| 6| 7| 7| 6| 6| 7| 5| 7| 7| 7| 6| 7| 7| 6| 6| 7| 7| 6| 7|            |
                             DAY ON TEST   | 3| 3| 1| 3| 3| 3| 1| 3| 3| 5| 0| 3| 6| 3| 3| 3| 6| 3| 3| 7| 2| 3| 3| 6| 2|            |
                                           | 1| 7| 8| 7| 7| 8| 7| 7| 1| 5| 4| 6| 8| 6| 6| 7| 3| 7| 7| 3| 7| 6| 6| 6| 9|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ENDOCRINE SYSTEM - cont                   |                                                                          |            |
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Accessory Adrenal Cortical Nodule    |                                                                          |      2  2.0|
      Cytoplasmic Alteration, Focal        |                                                                   1      |      1  1.0|
      Degeneration, Cystic                 |          1                                                               |      1  1.0|
      Infiltration Cellular, Mixed Cell    |                         2                                                |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  A  +  +  +  +  A|  46        |
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Cyst                                 |                X                                                         |      1     |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  M  +  +  +  +  +  +  +  +|  46        |
      Angiectasis                          |                                        1                                 |      2  1.0|
      Pars Distalis, Cytoplasmic           |                                                                          |            |
          Alteration, Focal                |                                                                          |      1  1.0|
      Pars Distalis, Hyperplasia, Focal    |                                                                          |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  A  +  A  +  +  +  +  +  +  +  +  +  +  +  A|  46        |
      Cyst                                 |                                                                          |      1     |
      Inflammation, Chronic, Focal         |                                                                          |      1  2.0|
      Follicle, Degeneration, Cystic, Focal|             1  1        2        2        2     2  1  1        2  2  2   |     17  1.8|
      Follicular Cell, Hyperplasia, Focal  | 1                                            1                 3         |      5  1.6|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Tissue NOS                              | +     +                                                                  |   3        |
      Pelvic, Fibrosis                     |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  10                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 6| 6| 7| 7| 6| 6| 7| 5| 7| 7| 7| 6| 7| 7| 6| 6| 7| 7| 6| 7|            |
                             DAY ON TEST   | 3| 3| 1| 3| 3| 3| 1| 3| 3| 5| 0| 3| 6| 3| 3| 3| 6| 3| 3| 7| 2| 3| 3| 6| 2|            |
                                           | 1| 7| 8| 7| 7| 8| 7| 7| 1| 5| 4| 6| 8| 6| 6| 7| 3| 7| 7| 3| 7| 6| 6| 6| 9|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  A|  47        |
      Angiectasis                          |                                                          3               |      3  3.3|
      Cyst                                 |                      X                             X  X     X        X   |     11     |
      Thrombosis                           |                                                                          |      3  4.0|
      Bilateral, Cyst                      |                                                                          |      1     |
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Angiectasis                          |                                                                          |      1  3.0|
      Inflammation, Suppurative            |                         3                                                |      1  3.0|
      Endometrium, Hyperplasia, Cystic     | 3  3  3  3  1  3  2  3  2  3  1  3  3  3  3  3  3  3  3  3  3  3  3  2  3|     48  2.8|
                                           |__________________________________________________________________________|____________|
   Vagina                                  |                                                                          |   1        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |          2              3           2        3  2                    3  3|     10  2.7|
                                           |__________________________________________________________________________|____________|
   Lymph Node                              | +     +                    +     +                                   +   |   7        |
      Iliac, Angiectasis                   | 3                                                                        |      1  3.0|
      Iliac, Hyperplasia, Lymphoid         |                            2                                             |      1  2.0|
      Mediastinal, Hyperplasia, Lymphoid   |                                  3                                       |      2  3.0|
      Renal, Hyperplasia, Lymphoid         |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Hemorrhage                           |                                                                          |      1  3.0|
      Hyperplasia, Lymphoid                |             2                    2  2                                    |      9  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  M  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I|  46        |
      Hemorrhage                           |                                                                          |      1  3.0|
      Hyperplasia, Lymphoid                |                         2        3                          2  3         |      6  2.3|
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  A  +  +  +  +  A|  47        |
      Hematopoietic Cell Proliferation     |          3           3  3  3     2     2     3  2     2              3   |     23  2.7|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  11                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 6| 6| 7| 7| 6| 6| 7| 5| 7| 7| 7| 6| 7| 7| 6| 6| 7| 7| 6| 7|            |
                             DAY ON TEST   | 3| 3| 1| 3| 3| 3| 1| 3| 3| 5| 0| 3| 6| 3| 3| 3| 6| 3| 3| 7| 2| 3| 3| 6| 2|            |
                                           | 1| 7| 8| 7| 7| 8| 7| 7| 1| 5| 4| 6| 8| 6| 6| 7| 3| 7| 7| 3| 7| 6| 6| 6| 9|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
      Hyperplasia, Lymphoid                |    2        2     3  3     3     2           3              3     2      |     17  2.5|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  I  +  M  +  +  +  +  +  +  +  +  +  +  +  I  A|  43        |
      Hyperplasia, Lymphoid                |    2     2                                            2           2      |      8  2.5|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperostosis                         |                                                             2            |      5  1.6|
      Vertebra, Hypertrophy, Focal         |                                                 2           1        2   |      3  1.7|
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         |                            +                                             |   1        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Compression, Focal                   |                                                 3                        |      1  3.0|
      Artery, Meninges, Inflammation,      |                                                                          |            |
          Chronic                          |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Peripheral Nerve                        |                                                                          |   1        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |                                                                          |   1        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Congestion                           |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  12                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 6| 6| 7| 7| 6| 6| 7| 5| 7| 7| 7| 6| 7| 7| 6| 6| 7| 7| 6| 7|            |
                             DAY ON TEST   | 3| 3| 1| 3| 3| 3| 1| 3| 3| 5| 0| 3| 6| 3| 3| 3| 6| 3| 3| 7| 2| 3| 3| 6| 2|            |
                                           | 1| 7| 8| 7| 7| 8| 7| 7| 1| 5| 4| 6| 8| 6| 6| 7| 3| 7| 7| 3| 7| 6| 6| 6| 9|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 RESPIRATORY SYSTEM - cont                 |                                                                          |            |
      Foreign Body                         |                                                                          |      1  3.0|
      Hemorrhage                           |                                                                          |      1  3.0|
      Hyperplasia, Histiocytic             |                                           1                              |      1  1.0|
      Infiltration Cellular,               |                                                                          |            |
          Polymorphonuclear                |                                                                         3|      1  3.0|
      Infiltration Cellular, Mixed Cell    |                                     3                                    |      1  3.0|
      Inflammation                         |                                                                          |      1  2.0|
      Metaplasia, Focal, Osseous           |                                                                          |      1  1.0|
      Alveolar Epithelium, Hyperplasia,    |                                                                          |            |
           Focal                           |                                                                          |      2  1.5|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  A  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  A  +  +  +  A  A|  43        |
      Anterior Chamber, Inflammation       |                                                 3                        |      1  3.0|
      Cornea, Inflammation                 |                                                 3                        |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Epithelium, Hyperplasia              |                   2                                                     1|      2  1.5|
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A|  48        |
      Hyperplasia, Lymphoid                |                                              2                           |      1  2.0|
      Metaplasia, Focal, Osseous           |                                                                          |      2  2.0|
      Nephropathy                          |    1           2                                                         |      6  1.3|
      Renal Tubule, Accumulation, Hyaline  |                                                                          |            |
          Droplet                          | 3                                                                        |      2  3.0|
      Renal Tubule, Cyst                   |                                                                          |      1     |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  13                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 6| 6| 7| 7| 6| 6| 7| 5| 7| 7| 7| 6| 7| 7| 6| 6| 7| 7| 6| 7|            |
                             DAY ON TEST   | 3| 3| 1| 3| 3| 3| 1| 3| 3| 5| 0| 3| 6| 3| 3| 3| 6| 3| 3| 7| 2| 3| 3| 6| 2|            |
                                           | 1| 7| 8| 7| 7| 8| 7| 7| 1| 5| 4| 6| 8| 6| 6| 7| 3| 7| 7| 3| 7| 6| 6| 6| 9|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 URINARY SYSTEM - cont                     |                                                                          |            |
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  A  +  A  +  +  +  +  +  +  A  +  +  +  I  +|  45        |
      Inflammation                         |                                                                          |      1  2.0|
      Transitional Epithelium, Hyperplasia |                                                                          |      1  2.0|
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  14                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 5| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 6| 5| 7| 7| 6| 5| 7|             
                             DAY ON TEST   | 3| 8| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 8| 3| 3| 7| 8| 3| 3| 1| 2| 3|             
                                           | 2| 9| 2| 2| 2| 7| 3| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 3| 5| 3| 3| 6| 1| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    2.5                                    | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |                   3                                                      |             
      Inflammation                         |                   3                                                      |             
      Perforation                          |                   3                                                      |             
                                           |__________________________________________________________________________|             
   Gallbladder                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  A  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  A  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
      Artery, Inflammation, Chronic, Focal |                                                          3               |             
                                           |__________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  A  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  A  +  +  A  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  A  +  +  A  +  +|             
      Epithelium, Hyperplasia, Focal       |                      2                                                   |             
                                           |__________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  A  +  +  A  +  +|             
                                           |__________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     |                                                                          |             
      Clear Cell Focus                     |                      X                                      X            |             
      Congestion                           |                                                                          |             
      Eosinophilic Focus                   |                                                                X         |             
      Hematopoietic Cell Proliferation     |                                              2                           |             
      Infiltration Cellular, Mixed Cell    |                                           1        2        1        1  1|             
      Mixed Cell Focus                     |                                        X                                 |             
      Hepatocyte, Necrosis, Focal          |             2                             1           2                  |             
      Hepatocyte, Vacuolization Cytoplasmic|                         2        2              1           3  2  3     2|             
                                           |__________________________________________________________________________|             
   Mesentery                               | +                    +        +     +        +        +  +               |             
      Infiltration Cellular, Lymphoid      |                      2                                                   |             
      Artery, Inflammation, Chronic, Focal |                                                          3               |             
      Fat, Necrosis, Focal                 | 3                    3        1     3        3                           |             
                                           |__________________________________________________________________________|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  15                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 5| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 6| 5| 7| 7| 6| 5| 7|             
                             DAY ON TEST   | 3| 8| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 8| 3| 3| 7| 8| 3| 3| 1| 2| 3|             
                                           | 2| 9| 2| 2| 2| 7| 3| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 3| 5| 3| 3| 6| 1| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    2.5                                    | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM - cont                  |                                                                          |             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  A  +  +  A  +  +|             
      Acinus, Atrophy, Focal               |                                     4                                    |             
                                           |__________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Lymphoid                |                                                                          |             
                                           |__________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Epithelium, Hyperplasia              |                                                                          |             
                                           |__________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  A  +  +|             
                                           |__________________________________________________________________________|             
   Tooth                                   |                         +                             +     +  +        +|             
      Dysplasia                            |                         1                             1     1  1        1|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Blood Vessel                            |                                                                          |             
      Aneurysm                             |                                                                          |             
                                           |__________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Accessory Adrenal Cortical Nodule    |                               1                             2            |             
                                           |__________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  A  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
                                           |__________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis                          |                                           1                              |             
      Pars Distalis, Cytoplasmic           |                                                                          |             
          Alteration, Focal                |             2                                                     1      |             
      Pars Distalis, Hyperplasia, Focal    |                                                                          |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  16                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 5| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 6| 5| 7| 7| 6| 5| 7|             
                             DAY ON TEST   | 3| 8| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 8| 3| 3| 7| 8| 3| 3| 1| 2| 3|             
                                           | 2| 9| 2| 2| 2| 7| 3| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 3| 5| 3| 3| 6| 1| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    2.5                                    | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
                                           |__________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic, Focal         |                                                          2               |             
      C-Cell, Hyperplasia                  |                                                                          |             
      Follicle, Degeneration, Cystic, Focal|    2  1                                1     1     2        2  1     2   |             
      Follicular Cell, Hyperplasia, Focal  | 2                                                                        |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |__________________________________________________________________________|             
   Peritoneum                              |                                              +                           |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration, Cystic                 |                                                                          |             
                                           |__________________________________________________________________________|             
   Ovary                                   | +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  +  +  +|             
      Angiectasis                          |                                        3                                 |             
      Cyst                                 |                                     X                    X               |             
      Interstitial Cell, Hyperplasia       |                                        3                                 |             
                                           |__________________________________________________________________________|             
   Oviduct                                 |                                                       +                  |             
                                           |__________________________________________________________________________|             
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis                          |                                           3                              |             
      Hemorrhage                           |                                                                          |             
      Inflammation, Chronic                |                                                                          |             
      Endometrium, Hyperplasia, Cystic     | 3  3  3  3  3  3     3     3  3  2  3  3  3  3  3  2  3  1  3  3  1  2  2|             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                2                                                         |             
                                           |__________________________________________________________________________|             
   Lymph Node                              |                                     +        +        +                  |             
      Pancreatic, Hyperplasia, Lymphoid    |                                     3                                    |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  17                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 5| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 6| 5| 7| 7| 6| 5| 7|             
                             DAY ON TEST   | 3| 8| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 8| 3| 3| 7| 8| 3| 3| 1| 2| 3|             
                                           | 2| 9| 2| 2| 2| 7| 3| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 3| 5| 3| 3| 6| 1| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    2.5                                    | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
      Hyperplasia, Lymphoid                | 4  2  2        3     3  3     2     2              3                     |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Ectasia                              |                                                                          |             
      Hemorrhage                           |                                                                          |             
      Hyperplasia, Lymphoid                |                      2              3  3                                 |             
                                           |__________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  A  +  +|             
      Angiectasis, Focal                   |          3                                                               |             
      Congestion                           |                               3                                          |             
      Fibrosis, Focal                      |          3                                                               |             
      Hematopoietic Cell Proliferation     | 2              3     3           2  2     3  2     3                     |             
      Hyperplasia, Lymphoid                | 2  2     2     2     3              3  3                                 |             
                                           |__________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Lymphoid                |                3     2     2        3  2                                2|             
      Inflammation                         |                   3                                                      |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperostosis                         |    1                                               1                     |             
      Vertebra, Hypertrophy, Focal         |                                                                      1   |             
                                           |__________________________________________________________________________|             
   Skeletal Muscle                         |    +                                               +                     |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Peripheral Nerve                        |    +                                                                     |             
                                           |__________________________________________________________________________|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  18                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 5| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 6| 5| 7| 7| 6| 5| 7|             
                             DAY ON TEST   | 3| 8| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 8| 3| 3| 7| 8| 3| 3| 1| 2| 3|             
                                           | 2| 9| 2| 2| 2| 7| 3| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 3| 5| 3| 3| 6| 1| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    2.5                                    | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 NERVOUS SYSTEM - cont                     |                                                                          |             
   Spinal Cord                             |    +                                                                     |             
      Demyelination, Focal                 |    3                                                                     |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                                          |             
      Mediastinum, Foreign Body            |                   X                                                      |             
      Mediastinum, Inflammation            |                   3                                                      |             
                                           |__________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                2                    2  2                                 |             
      Nasolacrimal Duct, Inflammation      |                                                                          |             
                                           |__________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +|             
      Artery, Retrobulbar, Inflammation,   |                                                                          |             
           Chronic, Focal                  |                                                          2               |             
      Cornea, Inflammation                 |                                              3                           |             
      Cornea, Necrosis, Focal              |                                              3                           |             
                                           |__________________________________________________________________________|             
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Epithelium, Hyperplasia              |       1                                                                  |             
                                           |__________________________________________________________________________|             
   Lacrimal Gland                          |                                                          +               |             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Infarct                              |                                                                          |             
      Metaplasia, Focal, Osseous           |                                                                          |             
      Nephropathy                          |                         2     1     1                                    |             
      Capsule, Fibrosis, Focal             |    2                                                                     |             
                                           |__________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  A  +  +|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  19                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 5| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 6| 5| 7| 7| 6| 5| 7|             
                             DAY ON TEST   | 3| 8| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 8| 3| 3| 7| 8| 3| 3| 1| 2| 3|             
                                           | 2| 9| 2| 2| 2| 7| 3| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 3| 5| 3| 3| 6| 1| 3|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    2.5                                    | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 URINARY SYSTEM - cont                     |                                                                          |             
      Hyperplasia, Lymphoid                |                                                                          |             
 __________________________________________|__________________________________________________________________________              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  20                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3|            |
                                           | 2| 8| 2| 2| 2| 2| 2| 2| 2| 3| 7| 7| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|      A     |
    2.5                                    | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Foreign Body                         |                                                                          |      1  3.0|
      Inflammation                         |                                                                          |      1  3.0|
      Perforation                          |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Gallbladder                             | +  +  +  +  +  +  +  +  +  A  +  +  M  +  +  +  +  +  +  +  A  +  +  +  +|  45        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Artery, Inflammation, Chronic, Focal |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +|  46        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +|  45        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +|  45        |
      Epithelium, Hyperplasia, Focal       |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  M  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +|  44        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Basophilic Focus                     |                                                                      X   |      1     |
      Clear Cell Focus                     | X                                                                        |      3     |
      Congestion                           |                            2                                            3|      2  2.5|
      Eosinophilic Focus                   |                                                                          |      1     |
      Hematopoietic Cell Proliferation     |                                                                          |      1  2.0|
      Infiltration Cellular, Mixed Cell    |          1  1  1  1     1           1                             2      |     12  1.2|
      Mixed Cell Focus                     |                                                                          |      1     |
      Hepatocyte, Necrosis, Focal          |                                                                          |      3  1.7|
      Hepatocyte, Vacuolization Cytoplasmic| 2                                            1     2     2               |     11  2.0|
                                           |__________________________________________________________________________|____________|
   Mesentery                               |       +                    +  +     +           +  +  +                  |  14        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  21                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3|            |
                                           | 2| 8| 2| 2| 2| 2| 2| 2| 2| 3| 7| 7| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|      A     |
    2.5                                    | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
      Infiltration Cellular, Lymphoid      |                                                                          |      1  2.0|
      Artery, Inflammation, Chronic, Focal |                                                                          |      1  3.0|
      Fat, Necrosis, Focal                 |       3                    3  2     3           3  3  3                  |     12  2.8|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +|  46        |
      Acinus, Atrophy, Focal               |                                                                          |      1  4.0|
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia, Lymphoid                |                                           3                              |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Epithelium, Hyperplasia              |    2                                                              2      |      2  2.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
                                           |__________________________________________________________________________|____________|
   Tooth                                   |                                  +           +  +     +              +   |  10        |
      Dysplasia                            |                                  2           1  2     1              1   |     10  1.2|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Blood Vessel                            |                                                    +                     |   1        |
      Aneurysm                             |                                                    4                     |      1  4.0|
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Accessory Adrenal Cortical Nodule    |                                                                          |      2  1.5|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +|  47        |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  22                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3|            |
                                           | 2| 8| 2| 2| 2| 2| 2| 2| 2| 3| 7| 7| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|      A     |
    2.5                                    | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ENDOCRINE SYSTEM - cont                   |                                                                          |            |
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Cyst                                 | X                                                                        |      1     |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Angiectasis                          |                                                                          |      1  1.0|
      Pars Distalis, Cytoplasmic           |                                                                          |            |
          Alteration, Focal                |                                        1                             2   |      4  1.5|
      Pars Distalis, Hyperplasia, Focal    |                                                                   2      |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation, Chronic, Focal         |                                                                          |      1  2.0|
      C-Cell, Hyperplasia                  |                                                                   2      |      1  2.0|
      Follicle, Degeneration, Cystic, Focal| 1                    1                          1  2  1        1  3      |     15  1.5|
      Follicular Cell, Hyperplasia, Focal  |       2                                                                  |      2  2.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Peritoneum                              |                                                                          |   1        |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Degeneration, Cystic                 |                                        2                                 |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Angiectasis                          |                                           3                              |      2  3.0|
      Cyst                                 |          X     X  X        X  X        X        X                 X  X  X|     12     |
      Interstitial Cell, Hyperplasia       |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Oviduct                                 |                                                                          |   1        |
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Angiectasis                          |                                                                          |      1  3.0|
      Hemorrhage                           | 3                                                                        |      1  3.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  23                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3|            |
                                           | 2| 8| 2| 2| 2| 2| 2| 2| 2| 3| 7| 7| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|      A     |
    2.5                                    | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
      Inflammation, Chronic                |                                           3                              |      1  3.0|
      Endometrium, Hyperplasia, Cystic     | 3  3  1  3  3  3  3  3  3     2  3     3  3  3  2  3  3  3  2  3  3  3   |     45  2.7|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                                  2                          2     2      |      4  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node                              |                            +              +                              |   5        |
      Pancreatic, Hyperplasia, Lymphoid    |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  +  +|  49        |
      Hyperplasia, Lymphoid                |       2                                                           3      |     11  2.6|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +|  49        |
      Ectasia                              |             3                                                            |      1  3.0|
      Hemorrhage                           |    3                                            3                        |      2  3.0|
      Hyperplasia, Lymphoid                |       2     2                 2                                2         |      7  2.3|
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +|  47        |
      Angiectasis, Focal                   |                                                                          |      1  3.0|
      Congestion                           |                                           3                              |      2  3.0|
      Fibrosis, Focal                      |                                                                          |      1  3.0|
      Hematopoietic Cell Proliferation     | 2     3  2        2     2  3     3  3  2                 2        3  2  3|     21  2.5|
      Hyperplasia, Lymphoid                |       2           2  3        2  2           2        2        2         |     15  2.3|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  I  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
      Hyperplasia, Lymphoid                |       2  2                          2                    2        2  2  2|     13  2.2|
      Inflammation                         |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  24                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3|            |
                                           | 2| 8| 2| 2| 2| 2| 2| 2| 2| 3| 7| 7| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|      A     |
    2.5                                    | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 INTEGUMENTARY SYSTEM - cont               |                                                                          |            |
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperostosis                         |          2                                   1                    2      |      5  1.4|
      Vertebra, Hypertrophy, Focal         |                            2                                             |      2  1.5|
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         |                            +                                            +|   4        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Peripheral Nerve                        |    +                                                                     |   2        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |    +                                                                     |   2        |
      Demyelination, Focal                 |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hemorrhage                           |                                     2                                    |      1  2.0|
      Mediastinum, Foreign Body            |                                                                          |      1     |
      Mediastinum, Inflammation            |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation                         |                                                                          |      3  2.0|
      Nasolacrimal Duct, Inflammation      |                            2                                             |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  25                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3|            |
                                           | 2| 8| 2| 2| 2| 2| 2| 2| 2| 3| 7| 7| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3|      A     |
    2.5                                    | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 SPECIAL SENSES SYSTEM - cont              |                                                                          |            |
   Eye                                     | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Artery, Retrobulbar, Inflammation,   |                                                                          |            |
           Chronic, Focal                  |                                                                          |      1  2.0|
      Cornea, Inflammation                 |                                                                          |      1  3.0|
      Cornea, Necrosis, Focal              |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Epithelium, Hyperplasia              |                                                                          |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Lacrimal Gland                          |                                                                          |   1        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Infarct                              |                                                          3               |      1  3.0|
      Metaplasia, Focal, Osseous           |                                                                      1   |      1  1.0|
      Nephropathy                          |                                           1              2               |      5  1.4|
      Capsule, Fibrosis, Focal             |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +|  47        |
      Hyperplasia, Lymphoid                |                                           3                              |      1  3.0|
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  26                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 5| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 3| 1| 3| 6| 3| 8| 3| 3| 3| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
                                           | 3| 3| 3| 6| 3| 8| 3| 3| 3| 3| 3| 3| 6| 3| 0| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    12.5                                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Gallbladder                             | +  +  +  +  +  A  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                               X                                          |             
                                           |__________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  A  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  A  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Lymphoid Tissue, Hyperplasia,        |                                                                          |             
          Lymphoid                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  A  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Epithelium, Hyperplasia, Focal       |                                                                          |             
                                           |__________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  A  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Lymphoid Tissue, Hyperplasia         |                                                                          |             
                                           |__________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  A  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Clear Cell Focus                     |             X                                                            |             
      Congestion                           |                                                                   1      |             
      Eosinophilic Focus                   |    X                                                                     |             
      Hematopoietic Cell Proliferation     |                                     2              1  1                  |             
      Infarct                              |                                           3                              |             
      Infiltration Cellular, Mixed Cell    | 2                          1  1  1  2     2  1              1  1         |             
      Hepatocyte, Necrosis, Focal          |                                                                          |             
      Hepatocyte, Vacuolization Cytoplasmic|             2     3     2                                            2   |             
      Kupffer Cell, Pigmentation           |                      1                                                   |             
                                           |__________________________________________________________________________|             
   Mesentery                               | +  +     +     +                          +           +           +      |             
      Hemorrhage                           |                                           3                              |             
      Artery, Inflammation, Chronic, Focal |                                                                          |             
      Fat, Necrosis, Focal                 | 1        3     3                          3           1           2      |             
                                           |__________________________________________________________________________|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  27                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 5| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 3| 1| 3| 6| 3| 8| 3| 3| 3| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
                                           | 3| 3| 3| 6| 3| 8| 3| 3| 3| 3| 3| 3| 6| 3| 0| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    12.5                                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM - cont                  |                                                                          |             
   Pancreas                                | +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acinus, Cytoplasmic Alteration       |                                                                      1   |             
      Duct, Cyst                           |       X                                X                                 |             
                                           |__________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Diverticulum                         |                               1                                          |             
      Inflammation, Focal                  |                                                 3                        |             
      Ulcer                                |                2           2                                             |             
      Epithelium, Hyperplasia              |                                                 3                        |             
                                           |__________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Tooth                                   |                               +                                          |             
      Dysplasia                            |                               1                                          |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Infiltration Cellular, Mixed Cell    |                                                                   3      |             
      Artery, Inflammation, Chronic        |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis                          |                                                                          |             
      Hyperplasia, Focal                   |                                                          2               |             
                                           |__________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +|             
      Hyperplasia, Focal                   |                                                                          |             
                                           |__________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +|             
      Cyst                                 |                            X                                             |             
                                           |__________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  M  +  +  +|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  28                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 5| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 3| 1| 3| 6| 3| 8| 3| 3| 3| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
                                           | 3| 3| 3| 6| 3| 8| 3| 3| 3| 3| 3| 3| 6| 3| 0| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    12.5                                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
      Cyst                                 |                                                                   X      |             
      Pars Distalis, Cytoplasmic           |                                                                          |             
          Alteration, Focal                |                                                                          |             
      Pars Distalis, Hyperplasia, Focal    |                                        2                                 |             
                                           |__________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                         X|             
      Inflammation, Chronic, Focal         |                                                                          |             
      Follicle, Degeneration, Cystic, Focal| 2  2     2              1           1  1     2  2     1  1     2     1   |             
      Follicular Cell, Hyperplasia, Focal  |                                        1                          3      |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Clitoral Gland                          | +  +  +  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration, Cystic                 |                2                                                         |             
                                           |__________________________________________________________________________|             
   Ovary                                   | M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |          X                             X        X                 X     X|             
      Hemorrhage                           |       3                                                                  |             
                                           |__________________________________________________________________________|             
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis                          |                                                                          |             
      Endometrium, Hyperplasia, Cystic     | 3  3  3  3  1  3  3     3  3  3  2  3  3  3  3  3  3  3  3  1  3  3  3  3|             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                   2  3  2           3        2     2                     |             
                                           |__________________________________________________________________________|             
   Lymph Node                              |    +           +                    +  +              +                  |             
      Iliac, Hyperplasia, Lymphoid         |                                                                          |             
      Pancreatic, Hyperplasia, Lymphoid    |                                                                          |             
      Renal, Hyperplasia, Lymphoid         |                                                                          |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  29                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 5| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 3| 1| 3| 6| 3| 8| 3| 3| 3| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
                                           | 3| 3| 3| 6| 3| 8| 3| 3| 3| 3| 3| 3| 6| 3| 0| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    12.5                                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
      Hyperplasia, Lymphoid                | 4     2                    2              2     2                        |             
      Hyperplasia, Mast Cell               |                                                                          |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Ectasia                              |                         3     3                                          |             
      Hemorrhage                           |                         3     3                                          |             
      Hyperplasia, Histiocytic             |                                              3                           |             
      Hyperplasia, Lymphoid                | 4                       3  2        2        2                           |             
                                           |__________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Fibrosis, Focal                      |                                              3                           |             
      Hematopoietic Cell Proliferation     | 3     2           2     2  2  2     3     3  3        2        2     3   |             
      Hyperplasia, Lymphoid                |       2  2  2     3     2                    3  3           2           2|             
                                           |__________________________________________________________________________|             
   Thymus                                  | +  +  I  +  +  +  +  I  +  +  +  +  I  +  +  +  +  +  +  +  +  +  I  +  +|             
      Hyperplasia, Lymphoid                | 4           2           4  2                 2                 2         |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperostosis                         |                2                                                  2     2|             
      Vertebra, Hypertrophy, Focal         |                                                                          |             
                                           |__________________________________________________________________________|             
   Skeletal Muscle                         |                                     +                                    |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Peripheral Nerve                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Spinal Cord                             |                                                                          |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  30                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 5| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 3| 1| 3| 6| 3| 8| 3| 3| 3| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
                                           | 3| 3| 3| 6| 3| 8| 3| 3| 3| 3| 3| 3| 6| 3| 0| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    12.5                                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |                      4                                                   |             
      Inflammation                         |                      4                                                   |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                                                    1                     |             
      Mediastinum, Foreign Body            |                      X                                                   |             
      Mediastinum, Inflammation            |                      3                                                   |             
      Serosa, Fibrosis                     |                                                 3                        |             
                                           |__________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                                     2  2                                 |             
                                           |__________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Eye                                     | +  +  +  +  +  A  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cornea, Inflammation                 |                            2                                             |             
                                           |__________________________________________________________________________|             
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Infarct                              |       2                                                        3         |             
      Metaplasia, Focal, Osseous           |                            2                                             |             
      Nephropathy                          |    1     1                    1           1                    2     1   |             
      Renal Tubule, Cyst                   |                                                                          |             
                                           |__________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  31                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 0| 3| 3| 2| 3| 3| 3| 3| 3|            |
                                           | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 7| 7| 5| 6| 7| 6| 5| 6| 6| 7| 3| 3| 3| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|      A     |
    12.5                                   | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Gallbladder                             | +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +|  47        |
      Cyst                                 |                                                                          |      1     |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +|  47        |
      Lymphoid Tissue, Hyperplasia,        |                                                                          |            |
          Lymphoid                         |                                           3                              |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +|  47        |
      Epithelium, Hyperplasia, Focal       |                                                                      2   |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +|  47        |
      Lymphoid Tissue, Hyperplasia         |                                                                      4   |      1  4.0|
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +|  47        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Clear Cell Focus                     |                                  X                    X     X            |      4     |
      Congestion                           |                                                                          |      1  1.0|
      Eosinophilic Focus                   |                               X                                      X   |      3     |
      Hematopoietic Cell Proliferation     |       2              1                    3                              |      6  1.7|
      Infarct                              |                                                                          |      1  3.0|
      Infiltration Cellular, Mixed Cell    |       2                                2     2                           |     12  1.5|
      Hepatocyte, Necrosis, Focal          |                                                 2                        |      1  2.0|
      Hepatocyte, Vacuolization Cytoplasmic|                                                             2  3     2  2|      8  2.3|
      Kupffer Cell, Pigmentation           |                                                                          |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Mesentery                               |                               +     +  +  +  +  +           +        +   |  15        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  32                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 0| 3| 3| 2| 3| 3| 3| 3| 3|            |
                                           | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 7| 7| 5| 6| 7| 6| 5| 6| 6| 7| 3| 3| 3| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|      A     |
    12.5                                   | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
      Hemorrhage                           |                                                                          |      1  3.0|
      Artery, Inflammation, Chronic, Focal |                                     3                                    |      1  3.0|
      Fat, Necrosis, Focal                 |                               3        3  1  3  3           3        1   |     13  2.3|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Acinus, Cytoplasmic Alteration       |                                                                          |      1  1.0|
      Duct, Cyst                           |                                                                          |      2     |
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Diverticulum                         |                                                                          |      1  1.0|
      Inflammation, Focal                  |                                                                          |      1  3.0|
      Ulcer                                |                                                 2                        |      3  2.0|
      Epithelium, Hyperplasia              |                                                 2                        |      2  2.5|
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Tooth                                   | +                                                           +            |   3        |
      Dysplasia                            | 1                                                           2            |      3  1.3|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Infiltration Cellular, Mixed Cell    |                                                                          |      1  3.0|
      Artery, Inflammation, Chronic        |                                     3                                    |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Angiectasis                          |                                                          2               |      1  2.0|
      Hyperplasia, Focal                   |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  33                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 0| 3| 3| 2| 3| 3| 3| 3| 3|            |
                                           | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 7| 7| 5| 6| 7| 6| 5| 6| 6| 7| 3| 3| 3| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|      A     |
    12.5                                   | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ENDOCRINE SYSTEM - cont                   |                                                                          |            |
      Hyperplasia, Focal                   |                               1                                          |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | M  +  +  M  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  46        |
      Cyst                                 |                                                                          |      1     |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Cyst                                 |                                                                          |      1     |
      Pars Distalis, Cytoplasmic           |                                                                          |            |
          Alteration, Focal                |                            2                                             |      1  2.0|
      Pars Distalis, Hyperplasia, Focal    |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Cyst                                 |                                                                          |      1     |
      Inflammation, Chronic, Focal         |                                     2                                    |      1  2.0|
      Follicle, Degeneration, Cystic, Focal|       2  1           2     2     2  1     2  2  2  1  2  3     2  1  1  1|     28  1.6|
      Follicular Cell, Hyperplasia, Focal  | 2                                            2                           |      4  2.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  I  I  +|  46        |
      Degeneration, Cystic                 |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Cyst                                 |    X  X        X  X  X           X  X     X  X  X        X               |     16     |
      Hemorrhage                           |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Angiectasis                          |                                                       3                  |      1  3.0|
      Endometrium, Hyperplasia, Cystic     | 3  3  3  3  3  3  3  3  3  3  3  3  1  3  1  3  2  2  3  3  3  3  3  3  3|     49  2.8|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  34                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 0| 3| 3| 2| 3| 3| 3| 3| 3|            |
                                           | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 7| 7| 5| 6| 7| 6| 5| 6| 6| 7| 3| 3| 3| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|      A     |
    12.5                                   | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Hyperplasia                          |       2                                2                 3               |      9  2.3|
                                           |__________________________________________________________________________|____________|
   Lymph Node                              | +     +                 +                 +                             +|  10        |
      Iliac, Hyperplasia, Lymphoid         |       3                                   2                              |      2  2.5|
      Pancreatic, Hyperplasia, Lymphoid    |                                                                         3|      1  3.0|
      Renal, Hyperplasia, Lymphoid         |       3                                                                  |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +|  48        |
      Hyperplasia, Lymphoid                |       1              2                                2  2  2     3  3   |     12  2.3|
      Hyperplasia, Mast Cell               |                                           3                              |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Ectasia                              |                                                                          |      2  3.0|
      Hemorrhage                           |                                                                          |      2  3.0|
      Hyperplasia, Histiocytic             |                                                                          |      1  3.0|
      Hyperplasia, Lymphoid                |       3                 3     2              2                       3   |     10  2.6|
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Fibrosis, Focal                      |                                                                          |      1  3.0|
      Hematopoietic Cell Proliferation     | 3  2  3  2  2  2     2  2     3  2     3  2     3  2  3  3     2     2  2|     31  2.4|
      Hyperplasia, Lymphoid                |          2           2                       2     2        3  2     2  2|     17  2.2|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  I  +  I  +  +  +  +  +  +  +  +  +  +  +|  44        |
      Hyperplasia, Lymphoid                |                2                             2     2        3        2   |     11  2.5|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  35                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 0| 3| 3| 2| 3| 3| 3| 3| 3|            |
                                           | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 7| 7| 5| 6| 7| 6| 5| 6| 6| 7| 3| 3| 3| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|      A     |
    12.5                                   | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 MUSCULOSKELETAL SYSTEM - cont             |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperostosis                         |       1                                                                  |      4  1.8|
      Vertebra, Hypertrophy, Focal         |                                                          2               |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         |       +                                         +                        |   3        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Peripheral Nerve                        |                                     +                                    |   1        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |                                     +                                    |   1        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Foreign Body                         |                                                                          |      1  4.0|
      Inflammation                         |                                                                          |      1  4.0|
      Alveolar Epithelium, Hyperplasia,    |                                                                          |            |
           Focal                           |                                                                          |      1  1.0|
      Mediastinum, Foreign Body            |                                                                          |      1     |
      Mediastinum, Inflammation            |                                                                          |      1  3.0|
      Serosa, Fibrosis                     |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation                         |       2              2                 2     2                       2   |      7  2.0|
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +|  47        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  36                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 0| 3| 3| 2| 3| 3| 3| 3| 3|            |
                                           | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 7| 7| 5| 6| 7| 6| 5| 6| 6| 7| 3| 3| 3| 3| 3|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|      A     |
    12.5                                   | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 SPECIAL SENSES SYSTEM - cont              |                                                                          |            |
      Cornea, Inflammation                 |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Infarct                              |                                                                          |      2  2.5|
      Metaplasia, Focal, Osseous           |                                                                          |      1  2.0|
      Nephropathy                          |             1                                                  1        1|      9  1.1|
      Renal Tubule, Cyst                   |       X  X                                                              X|      3     |
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +|  49        |
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  37                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 5| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3|             
                                           | 3| 3| 3| 3| 3| 2| 2| 2| 2| 2| 2| 2| 2| 2| 9| 6| 6| 6| 6| 6| 9| 6| 6| 6| 6|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    25                                     | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Gallbladder                             | +  +  +  +  +  +  +  +  A  +  +  +  +  +  A  +  +  +  +  +  A  +  +  +  +|             
      Cyst                                 |                                                                          |             
                                           |__________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  A  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  M  +  +  +  +  +  A  +  +  +  +  +  A  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  A  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  A  +  +  +  +  +  A  +  +  +  +  +  A  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  A  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  A  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     |                                                                X         |             
      Clear Cell Focus                     | X                       X                                                |             
      Congestion                           |                         3                                                |             
      Eosinophilic Focus                   |                         X                                                |             
      Hematopoietic Cell Proliferation     |       1  1                                                               |             
      Hemorrhage                           |                                     1                                    |             
      Infiltration Cellular, Mixed Cell    | 1     1  1     1           1           2     1     1  1  1               |             
      Mixed Cell Focus                     |                                                                          |             
      Hepatocyte, Vacuolization Cytoplasmic|    3                    3           3  3  3     2  2           2  2      |             
                                           |__________________________________________________________________________|             
   Mesentery                               |                         +                 +              +  +     +      |             
      Hemorrhage                           |                                                                          |             
      Fat, Necrosis, Focal                 |                                           1                       3      |             
                                           |__________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acinus, Atrophy, Diffuse             |             4                                                            |             
      Acinus, Atrophy, Focal               | 1                                                                        |             
      Duct, Cyst                           |             X                                                            |             
                                           |__________________________________________________________________________|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  38                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 5| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3|             
                                           | 3| 3| 3| 3| 3| 2| 2| 2| 2| 2| 2| 2| 2| 2| 9| 6| 6| 6| 6| 6| 9| 6| 6| 6| 6|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    25                                     | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM - cont                  |                                                                          |             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Diverticulum                         |    1                       1                                             |             
      Ulcer                                |                                                                          |             
      Epithelium, Hyperplasia              |                                                                          |             
                                           |__________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
                                           |__________________________________________________________________________|             
   Tooth                                   |    +                                                     +               |             
      Dysplasia                            |    1                                                     2               |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       |                                                                          |             
      Mineralization, Focal                |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
                                           |__________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
      Pars Distalis, Cytoplasmic           |                                                                          |             
          Alteration, Focal                | 2                                                                        |             
      Pars Distalis, Hyperplasia, Focal    |                                                                          |             
                                           |__________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +|             
      C-Cell, Hyperplasia                  |    2                                                                     |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  39                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 5| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3|             
                                           | 3| 3| 3| 3| 3| 2| 2| 2| 2| 2| 2| 2| 2| 2| 9| 6| 6| 6| 6| 6| 9| 6| 6| 6| 6|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    25                                     | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
      Follicle, Degeneration, Cystic, Focal|    2  2  2  2     2                 2  2  1  1  2  1     2     1  1  1   |             
      Follicular Cell, Hyperplasia, Focal  |                            1                                            1|             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |__________________________________________________________________________|             
   Tissue NOS                              |                         +                                                |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis                          |       3  2                                                               |             
      Cyst                                 |                                                       X     X        X  X|             
      Hemorrhage                           |                                                                          |             
      Pigmentation, Focal                  |       3                                                                  |             
      Thrombosis                           |       3                                                                  |             
                                           |__________________________________________________________________________|             
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Endometrium, Hyperplasia, Cystic     | 3  3  3  3  3  3  3  3  1  3  3  4  2  3  2  3  3  3  3  3  3  3  3  3  3|             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                                          3  2            |             
                                           |__________________________________________________________________________|             
   Lymph Node                              |                         +                                +               |             
      Iliac, Hyperplasia, Lymphoid         |                                                          3               |             
      Mediastinal, Hyperplasia, Lymphoid   |                                                          3               |             
      Mediastinal, Inflammation            |                                                                          |             
      Pancreatic, Hyperplasia, Lymphoid    |                                                                          |             
      Renal, Hyperplasia, Lymphoid         |                                                                          |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Lymphoid                |    2     2        2        2                 2  2  2              2  2  2|             
                                           |__________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                             3            |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  40                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 5| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3|             
                                           | 3| 3| 3| 3| 3| 2| 2| 2| 2| 2| 2| 2| 2| 2| 9| 6| 6| 6| 6| 6| 9| 6| 6| 6| 6|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    25                                     | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
      Hyperplasia, Lymphoid                |                               2                 2                 2  3   |             
                                           |__________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hematopoietic Cell Proliferation     | 2  2  3  2  2  3  2  2  3  2  2  2  2  3        2  3     3  3  2  2  2  2|             
      Hyperplasia, Lymphoid                |       3                    2  2                       2           3  2   |             
                                           |__________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +|             
      Hyperplasia, Lymphoid                |    2                          2                    2           2  2     2|             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Subcutaneous Tissue, Edema           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperostosis                         |                   2              2                                       |             
      Vertebra, Hypertrophy, Focal         |                                                                          |             
                                           |__________________________________________________________________________|             
   Skeletal Muscle                         |                                     +                    +  +            |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Compression, Focal                   |                                                                         3|             
                                           |__________________________________________________________________________|             
   Peripheral Nerve                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Spinal Cord                             |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Larynx                                  |                               M                                          |             
                                           |__________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  41                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 5| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3|             
                                           | 3| 3| 3| 3| 3| 2| 2| 2| 2| 2| 2| 2| 2| 2| 9| 6| 6| 6| 6| 6| 9| 6| 6| 6| 6|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    25                                     | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
      Hyperplasia, Histiocytic             |                                                                          |             
      Hyperplasia, Lymphoid                |                                                                          |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                                                                          |             
                                           |__________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                         2           1                          2     2   |             
      Nasolacrimal Duct, Hyperplasia, Focal|          2                                                               |             
                                           |__________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |                                                                          |             
      Cataract                             |                                                                          |             
      Cornea, Inflammation                 |                                                                   3      |             
                                           |__________________________________________________________________________|             
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Epithelium, Hyperplasia              |       3              2                                                   |             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                                          |             
      Infarct                              | 2                                                                        |             
      Nephropathy                          |                                        1     1  1                        |             
      Bilateral, Infarct                   |                                                                          |             
      Renal Tubule, Accumulation, Hyaline  |                                                                          |             
          Droplet                          |                         3                                                |             
                                           |__________________________________________________________________________|             
   Ureter                                  |                               +                                          |             
                                           |__________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  +|             
      Transitional Epithelium, Hyperplasia | 2                                                                        |             
 __________________________________________|__________________________________________________________________________              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  42                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 5| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 2| 2| 2| 2| 2| 3| 4| 3| 3| 3| 7| 3| 8| 2| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3|            |
                                           | 9| 9| 9| 9| 9| 2| 9| 2| 2| 2| 1| 3| 0| 8| 3| 7| 7| 7| 5| 7| 7| 2| 7| 7| 7|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|      A     |
    25                                     | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Gallbladder                             | +  +  +  +  +  +  A  +  +  +  A  +  +  M  +  +  +  +  +  +  +  +  +  +  +|  44        |
      Cyst                                 |    X                       X                                             |      2     |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|  47        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|  46        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  A  +  +  +  A  +  +  A  +  +  +  +  A  +  +  +  +  +  +|  44        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  A  +  +  +  A  +  +  A  +  +  +  +  A  +  +  A  +  +  +|  42        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  A  +  +  +  A  +  +  A  +  +  +  +  A  +  +  A  +  +  +|  43        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  A  +  +  +  A  +  +  A  +  +  +  +  A  +  +  A  +  +  +|  43        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Basophilic Focus                     | X                                                  X                     |      3     |
      Clear Cell Focus                     |                                                                          |      2     |
      Congestion                           |                                                                          |      1  3.0|
      Eosinophilic Focus                   |                                                                          |      1     |
      Hematopoietic Cell Proliferation     |                                                                          |      2  1.0|
      Hemorrhage                           |                                                                          |      1  1.0|
      Infiltration Cellular, Mixed Cell    |          1              1                          1     1  1            |     15  1.1|
      Mixed Cell Focus                     |                                                             X            |      1     |
      Hepatocyte, Vacuolization Cytoplasmic|       2     2                             2     2                 2  1   |     15  2.3|
                                           |__________________________________________________________________________|____________|
   Mesentery                               |                   +           +  +  +              +  +        +     +   |  13        |
      Hemorrhage                           |                                     2                                    |      1  2.0|
      Fat, Necrosis, Focal                 |                                     3              3                     |      4  2.5|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|  49        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  43                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 5| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 2| 2| 2| 2| 2| 3| 4| 3| 3| 3| 7| 3| 8| 2| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3|            |
                                           | 9| 9| 9| 9| 9| 2| 9| 2| 2| 2| 1| 3| 0| 8| 3| 7| 7| 7| 5| 7| 7| 2| 7| 7| 7|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|      A     |
    25                                     | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
      Acinus, Atrophy, Diffuse             |                                                                          |      1  4.0|
      Acinus, Atrophy, Focal               |                                                                          |      1  1.0|
      Duct, Cyst                           |          X                                                               |      2     |
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Diverticulum                         |          1                                                               |      3  1.0|
      Ulcer                                |                   1                                                      |      1  1.0|
      Epithelium, Hyperplasia              |                   2                                                      |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  A  +  +  +  A  +  +  +  +  +  +  +  +  +  +  A  +  +  +|  46        |
      Cyst                                 |                                                                      X   |      1     |
                                           |__________________________________________________________________________|____________|
   Tooth                                   |                                     +                                    |   3        |
      Dysplasia                            |                                     1                                    |      3  1.3|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cardiomyopathy                       |                                     1                                    |      1  1.0|
      Mineralization, Focal                |                   1                                                      |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  A  +  +  +|  48        |
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Cyst                                 |       X                                                                  |      1     |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  44                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 5| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 2| 2| 2| 2| 2| 3| 4| 3| 3| 3| 7| 3| 8| 2| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3|            |
                                           | 9| 9| 9| 9| 9| 2| 9| 2| 2| 2| 1| 3| 0| 8| 3| 7| 7| 7| 5| 7| 7| 2| 7| 7| 7|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|      A     |
    25                                     | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ENDOCRINE SYSTEM - cont                   |                                                                          |            |
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  M|  47        |
      Cyst                                 |             X                                                            |      1     |
      Pars Distalis, Cytoplasmic           |                                                                          |            |
          Alteration, Focal                |                2                                                         |      2  2.0|
      Pars Distalis, Hyperplasia, Focal    |                                                                   2      |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
      C-Cell, Hyperplasia                  |                                                                          |      1  2.0|
      Follicle, Degeneration, Cystic, Focal| 2     2           2  2              1     2                       2  2   |     23  1.7|
      Follicular Cell, Hyperplasia, Focal  |             1                                            2           2   |      5  1.4|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Tissue NOS                              |                               +                       +                  |   3        |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|  48        |
      Angiectasis                          |                                                                          |      2  2.5|
      Cyst                                 | X     X                 X        X  X  X  X        X              X      |     13     |
      Hemorrhage                           |                                     3                                    |      1  3.0|
      Pigmentation, Focal                  |                                                                          |      1  3.0|
      Thrombosis                           |                                     3  4                                 |      3  3.3|
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Endometrium, Hyperplasia, Cystic     | 3  2  3  3  3  3  2  3  2  3     3  3  1  3  3  3  3     1  3  2  3  3  3|     48  2.8|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                                     2           3                        |      4  2.5|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  45                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 5| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 2| 2| 2| 2| 2| 3| 4| 3| 3| 3| 7| 3| 8| 2| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3|            |
                                           | 9| 9| 9| 9| 9| 2| 9| 2| 2| 2| 1| 3| 0| 8| 3| 7| 7| 7| 5| 7| 7| 2| 7| 7| 7|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|      A     |
    25                                     | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
   Lymph Node                              |                +  +           +     +        +        +        +     +   |  10        |
      Iliac, Hyperplasia, Lymphoid         |                                     2                                    |      2  2.5|
      Mediastinal, Hyperplasia, Lymphoid   |                                                                          |      1  3.0|
      Mediastinal, Inflammation            |                                     3                                    |      1  3.0|
      Pancreatic, Hyperplasia, Lymphoid    |                                              3                           |      1  3.0|
      Renal, Hyperplasia, Lymphoid         |                                     3                                    |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  M  +  +  +  +  +  +  I  +  +|  46        |
      Hyperplasia, Lymphoid                |          2                 2        3  2        3        3               |     16  2.2|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  I  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Hemorrhage                           |                                                                          |      1  3.0|
      Hyperplasia, Lymphoid                |          2                                      3  2                    2|      8  2.3|
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hematopoietic Cell Proliferation     | 2  3  2  2  2  2        3  2     3  3  3        3  2     2  2     2  2  2|     40  2.3|
      Hyperplasia, Lymphoid                |       2                    2                 3  3        3               |     11  2.5|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Hyperplasia, Lymphoid                |    2  2                    2        3     2  2  2        2               |     14  2.1|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Subcutaneous Tissue, Edema           |                   3                                                      |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperostosis                         |                                           2        2                 3   |      5  2.2|
      Vertebra, Hypertrophy, Focal         |                                                       2                  |      1  2.0|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  46                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 5| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 2| 2| 2| 2| 2| 3| 4| 3| 3| 3| 7| 3| 8| 2| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3|            |
                                           | 9| 9| 9| 9| 9| 2| 9| 2| 2| 2| 1| 3| 0| 8| 3| 7| 7| 7| 5| 7| 7| 2| 7| 7| 7|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|      A     |
    25                                     | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 MUSCULOSKELETAL SYSTEM - cont             |                                                                          |            |
   Skeletal Muscle                         |                                                                          |   3        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +|  49        |
      Compression, Focal                   |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Peripheral Nerve                        |                                     +                                    |   1        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |                                     +                                    |   1        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Larynx                                  |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia, Histiocytic             |                                                                3         |      1  3.0|
      Hyperplasia, Lymphoid                |                                        3                                 |      1  3.0|
      Alveolar Epithelium, Hyperplasia,    |                                                                          |            |
           Focal                           |                                  2                                       |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation                         | 2              2        2                 2           2  2  2            |     11  1.9|
      Nasolacrimal Duct, Hyperplasia, Focal|                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  A  +  +  +  A  +  +  +  +  +  +  +  +  +  +  A  +  +  +|  47        |
      Atrophy                              |                                                       4                  |      1  4.0|
      Cataract                             |                                                       3                  |      1  3.0|
      Cornea, Inflammation                 |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  47                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 5| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7|            |
                             DAY ON TEST   | 2| 2| 2| 2| 2| 3| 4| 3| 3| 3| 7| 3| 8| 2| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3|            |
                                           | 9| 9| 9| 9| 9| 2| 9| 2| 2| 2| 1| 3| 0| 8| 3| 7| 7| 7| 5| 7| 7| 2| 7| 7| 7|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE FEMALE                      | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|      A     |
    25                                     | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 SPECIAL SENSES SYSTEM - cont              |                                                                          |            |
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Epithelium, Hyperplasia              |          2                                                               |      3  2.3|
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hemorrhage                           |                                                                3         |      1  3.0|
      Infarct                              |                                     2                                    |      2  2.0|
      Nephropathy                          |    1                                2                                    |      5  1.2|
      Bilateral, Infarct                   |                                                                4         |      1  4.0|
      Renal Tubule, Accumulation, Hyaline  |                                                                          |            |
          Droplet                          |                         2                                                |      2  2.5|
                                           |__________________________________________________________________________|____________|
   Ureter                                  |                                                                          |   1        |
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Transitional Epithelium, Hyperplasia |                                                                          |      1  2.0|
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  48                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 6| 7| 7| 7| 7| 4| 6| 6| 5| 7| 7| 5| 7|             
                             DAY ON TEST   | 9| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 2| 7| 0| 6| 4| 2| 2| 4| 2|             
                                           | 4| 0| 1| 0| 0| 0| 0| 6| 0| 2| 0| 1| 0| 9| 1| 0| 3| 8| 5| 5| 8| 9| 9| 4| 9|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Gallbladder                             | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  A  +  +  +  M|             
                                           |__________________________________________________________________________|             
   Intestine Large, Colon                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Rectum                 | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Cecum                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Duodenum               | A  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  A  +  +  +  I  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Jejunum                | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +|             
      Inflammation                         |                                                                2         |             
      Lymphoid Tissue, Hyperplasia         |                                                                          |             
                                           |__________________________________________________________________________|             
   Intestine Small, Ileum                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  I  A  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Clear Cell Focus                     |    X  X  X  X  X           X     X        X                       X     X|             
      Fibrosis, Focal                      |                                                                          |             
      Hematopoietic Cell Proliferation     |                                                                          |             
      Infarct                              |                                                          4               |             
      Infiltration Cellular, Mixed Cell    |                                        1                                 |             
      Mixed Cell Focus                     |          X        X                                                      |             
      Hepatocyte, Necrosis, Focal          |                                                                      2   |             
      Hepatocyte, Vacuolization Cytoplasmic|    2  2  2  2  2        2  1              2  2              3  2  3     3|             
                                           |__________________________________________________________________________|             
   Mesentery                               |                                        +                 +               |             
      Fat, Necrosis, Focal                 |                                        4                 3               |             
                                           |__________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +|             
      Acinus, Atrophy, Diffuse             |                                                                          |             
      Acinus, Atrophy, Focal               |                                                                          |             
      Acinus, Cytoplasmic Alteration       |                                                                          |             
                                           |__________________________________________________________________________|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  49                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 6| 7| 7| 7| 7| 4| 6| 6| 5| 7| 7| 5| 7|             
                             DAY ON TEST   | 9| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 2| 7| 0| 6| 4| 2| 2| 4| 2|             
                                           | 4| 0| 1| 0| 0| 0| 0| 6| 0| 2| 0| 1| 0| 9| 1| 0| 3| 8| 5| 5| 8| 9| 9| 4| 9|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM - cont                  |                                                                          |             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Epithelium, Hyperplasia              |                                                 2                    2   |             
                                           |__________________________________________________________________________|             
   Stomach, Glandular                      | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +|             
      Erosion                              |                                                          2           2   |             
      Ulcer                                |                                                                          |             
                                           |__________________________________________________________________________|             
   Tooth                                   | +  +  +  +  +  +  +     +  +  +  +     +  +  +  +        +     +  +  +  +|             
      Dysplasia                            | 2  4  4  4  4  4  1     3  4  2  4     3  1  4  3        4     2  2  2  3|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Infiltration Cellular, Mixed Cell    |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Accessory Adrenal Cortical Nodule    |          1                                                               |             
      Cytoplasmic Alteration, Focal        |          2                                                  1            |             
      Degeneration, Cystic                 |                                                                          |             
      Hypertrophy                          |                                                                          |             
      Inflammation                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |             1                                                            |             
                                           |__________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |       2  2  2  2           2     2     2  2     3     2     2           2|             
                                           |__________________________________________________________________________|             
   Parathyroid Gland                       | +  M  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  M  +|             
                                           |__________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                               X           X                              |             
                                           |__________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  50                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 6| 7| 7| 7| 7| 4| 6| 6| 5| 7| 7| 5| 7|             
                             DAY ON TEST   | 9| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 2| 7| 0| 6| 4| 2| 2| 4| 2|             
                                           | 4| 0| 1| 0| 0| 0| 0| 6| 0| 2| 0| 1| 0| 9| 1| 0| 3| 8| 5| 5| 8| 9| 9| 4| 9|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
      Follicle, Degeneration, Cystic, Focal|          2        2              1                    1  1               |             
      Follicular Cell, Hyperplasia, Focal  |    3  1  1              2                       2                        |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |__________________________________________________________________________|             
   Tissue NOS                              |                                                                          |             
      Pelvic, Inflammation                 |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration, Cystic                 |       3     3           3                                3        3      |             
      Inflammation, Chronic                |                                                 3                        |             
                                           |__________________________________________________________________________|             
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                                        2                                 |             
      Epithelium, Hyperplasia              |                3                                                         |             
                                           |__________________________________________________________________________|             
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Dilatation                           |             3                    3                                       |             
                                           |__________________________________________________________________________|             
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis                          |                                                                          |             
      Atrophy                              |                                                                          |             
      Thrombosis                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                                                      3   |             
                                           |__________________________________________________________________________|             
   Lymph Node                              |                                                                +         |             
      Inguinal, Hyperplasia, Lymphoid      |                                                                          |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mandibular                  | A  +  +  M  +  M  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Lymphoid                |                                                    3                     |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  51                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 6| 7| 7| 7| 7| 4| 6| 6| 5| 7| 7| 5| 7|             
                             DAY ON TEST   | 9| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 2| 7| 0| 6| 4| 2| 2| 4| 2|             
                                           | 4| 0| 1| 0| 0| 0| 0| 6| 0| 2| 0| 1| 0| 9| 1| 0| 3| 8| 5| 5| 8| 9| 9| 4| 9|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mesenteric                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +|             
      Hyperplasia, Lymphoid                |                                                                          |             
                                           |__________________________________________________________________________|             
   Spleen                                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hematopoietic Cell Proliferation     |                                  2  3              3     3               |             
      Hyperplasia, Lymphoid                |       2                    2                                             |             
      Hyperplasia, Mast Cell               |                                                                          |             
      Lymphoid Follicle, Necrosis          |                                                                          |             
                                           |__________________________________________________________________________|             
   Thymus                                  | I  M  +  +  +  I  +  +  +  +  +  I  I  +  +  M  I  +  I  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|             
                                           |__________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst Epithelial Inclusion            |                                                                          |             
      Subcutaneous Tissue, Edema           |                                                          2               |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Peripheral Nerve                        |                                  +                                       |             
                                           |__________________________________________________________________________|             
   Spinal Cord                             |                                  +                                       |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                       2                  |             
      Hyperplasia, Histiocytic             |                                                 2                        |             
      Inflammation                         |                                                                          |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  52                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 6| 7| 7| 7| 7| 4| 6| 6| 5| 7| 7| 5| 7|             
                             DAY ON TEST   | 9| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 2| 7| 0| 6| 4| 2| 2| 4| 2|             
                                           | 4| 0| 1| 0| 0| 0| 0| 6| 0| 2| 0| 1| 0| 9| 1| 0| 3| 8| 5| 5| 8| 9| 9| 4| 9|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    0 MG/KG                                | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                2                          2                              |             
      Mediastinum, Foreign Body            |                                                       X                  |             
      Mediastinum, Inflammation,           |                                                                          |             
          Suppurative                      |                                                       3                  |             
      Mediastinum, Thrombosis              |    4                                                                     |             
                                           |__________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |       2                                                                  |             
      Nasolacrimal Duct, Inflammation      | 2                                2                                       |             
                                           |__________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Eye                                     | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +|             
      Cornea, Inflammation                 |                                  2                                       |             
      Cornea, Necrosis, Focal              |                                                                          |             
                                           |__________________________________________________________________________|             
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                                                                          |             
      Epithelium, Hyperplasia              |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Kidney                                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hydronephrosis                       |                                                                          |             
      Metaplasia, Focal, Osseous           |             1                                                           2|             
      Nephropathy                          |    2  1  1  1  1  2  2  2  2  2  2  3  2  1  2  2     1  2     1        1|             
      Renal Tubule, Cyst                   |                                  X                                X      |             
      Renal Tubule, Hyperplasia, Focal     |                                           1  2                 1         |             
                                           |__________________________________________________________________________|             
   Urethra                                 |                                                                          |             
      Inflammation, Chronic                |                                                                          |             
                                           |__________________________________________________________________________|             
   Urinary Bladder                         | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Transitional Epithelium, Hyperplasia |                                                                          |             
 __________________________________________|__________________________________________________________________________              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  53                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 5| 6| 7| 7|            |
                             DAY ON TEST   | 2| 0| 3| 3| 3| 2| 7| 3| 3| 2| 3| 2| 3| 2| 3| 2| 3| 3| 2| 2| 2| 5| 0| 3| 3|            |
                                           | 3| 5| 0| 1| 1| 9| 3| 0| 0| 9| 0| 9| 0| 9| 0| 9| 0| 0| 9| 9| 9| 9| 5| 1| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation                         |                                                                   3      |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Gallbladder                             | A  +  +  +  +  +  M  +  +  +  +  +  +  +  +  M  I  +  +  +  +  A  A  +  +|  40        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  A  +  +  +|  45        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  A  M  +  +|  42        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  A  +  +  +|  45        |
      Inflammation                         |                                                                          |      1  2.0|
      Lymphoid Tissue, Hyperplasia         |                                                             3            |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  A  +  +  +|  43        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Clear Cell Focus                     |                X           X  X     X              X  X     X     X  X  X|     20     |
      Fibrosis, Focal                      |                                           3                              |      1  3.0|
      Hematopoietic Cell Proliferation     |    1                                                                     |      1  1.0|
      Infarct                              |                                                                          |      1  4.0|
      Infiltration Cellular, Mixed Cell    |    2     2                                                               |      3  1.7|
      Mixed Cell Focus                     |                                                          X               |      3     |
      Hepatocyte, Necrosis, Focal          |                                           3                              |      2  2.5|
      Hepatocyte, Vacuolization Cytoplasmic|             2  2     2  2  3  2  3  3        2     3  2  2  3     2  3  3|     29  2.3|
                                           |__________________________________________________________________________|____________|
   Mesentery                               |                   +                                   +  +               |   5        |
      Fat, Necrosis, Focal                 |                                                       3  2               |      4  3.0|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  54                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 5| 6| 7| 7|            |
                             DAY ON TEST   | 2| 0| 3| 3| 3| 2| 7| 3| 3| 2| 3| 2| 3| 2| 3| 2| 3| 3| 2| 2| 2| 5| 0| 3| 3|            |
                                           | 3| 5| 0| 1| 1| 9| 3| 0| 0| 9| 0| 9| 0| 9| 0| 9| 0| 0| 9| 9| 9| 9| 5| 1| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
      Acinus, Atrophy, Diffuse             |                                           4                              |      1  4.0|
      Acinus, Atrophy, Focal               |                                     2                                    |      1  2.0|
      Acinus, Cytoplasmic Alteration       |                      1                                                   |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Epithelium, Hyperplasia              |                   2                                                      |      3  2.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +|  47        |
      Erosion                              |                                                                          |      2  2.0|
      Ulcer                                |    3                                                                     |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Tooth                                   | +  +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +  +  +  +     +  +  +|  43        |
      Dysplasia                            | 1  2  1  4  2  2  2  4  2  4  3  2  2     2  1  4  4  2  3  3     2  2  3|     43  2.7|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Infiltration Cellular, Mixed Cell    |                               2                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +|  48        |
      Accessory Adrenal Cortical Nodule    |                                                                          |      1  1.0|
      Cytoplasmic Alteration, Focal        |                               1                          2               |      4  1.5|
      Degeneration, Cystic                 |          2                                                               |      1  2.0|
      Hypertrophy                          |                         1                                                |      1  1.0|
      Inflammation                         |    3                                                                     |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +|  48        |
      Hyperplasia, Focal                   |                                                          2               |      2  1.5|
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  55                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 5| 6| 7| 7|            |
                             DAY ON TEST   | 2| 0| 3| 3| 3| 2| 7| 3| 3| 2| 3| 2| 3| 2| 3| 2| 3| 3| 2| 2| 2| 5| 0| 3| 3|            |
                                           | 3| 5| 0| 1| 1| 9| 3| 0| 0| 9| 0| 9| 0| 9| 0| 9| 0| 0| 9| 9| 9| 9| 5| 1| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ENDOCRINE SYSTEM - cont                   |                                                                          |            |
      Hyperplasia                          |                         2                 2  2  3                       2|     17  2.1|
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  46        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |                                                       X                  |      3     |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Follicle, Degeneration, Cystic, Focal|                               2                                          |      6  1.5|
      Follicular Cell, Hyperplasia, Focal  |       2  2                       2     1  1  2     2  2     1            |     14  1.7|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Tissue NOS                              | +                                                                        |   1        |
      Pelvic, Inflammation                 | 3                                                                        |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Degeneration, Cystic                 |       3              3                       3     3           3  3      |     11  3.0|
      Inflammation, Chronic                | 3                                                                        |      2  3.0|
                                           |__________________________________________________________________________|____________|
   Prostate                                | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Inflammation                         |                                                                          |      1  2.0|
      Epithelium, Hyperplasia              |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Seminal Vesicle                         | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Dilatation                           |                                                                          |      2  3.0|
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Angiectasis                          |                                                    2                     |      1  2.0|
      Atrophy                              | 2                                                                        |      1  2.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  56                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 5| 6| 7| 7|            |
                             DAY ON TEST   | 2| 0| 3| 3| 3| 2| 7| 3| 3| 2| 3| 2| 3| 2| 3| 2| 3| 3| 2| 2| 2| 5| 0| 3| 3|            |
                                           | 3| 5| 0| 1| 1| 9| 3| 0| 0| 9| 0| 9| 0| 9| 0| 9| 0| 0| 9| 9| 9| 9| 5| 1| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
      Thrombosis                           |                                                    2                     |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                                                          3               |      2  3.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node                              |       +  +                 +                             +               |   5        |
      Inguinal, Hyperplasia, Lymphoid      |                                                          3               |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | M  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +|  43        |
      Hyperplasia, Lymphoid                |                                                          3               |      2  3.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
      Hyperplasia, Lymphoid                |                                                          4  3            |      2  3.5|
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Hematopoietic Cell Proliferation     | 2  2     3     3  3                 2  3     2           3     2         |     14  2.6|
      Hyperplasia, Lymphoid                |             2                    2  2                    2  2            |      7  2.0|
      Hyperplasia, Mast Cell               |                                        2                                 |      1  2.0|
      Lymphoid Follicle, Necrosis          |                                                                   3      |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | I  +  +  +  +  +  +  +  +  I  +  +  I  I  +  +  +  +  +  +  +  +  +  +  +|  38        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|            |
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst Epithelial Inclusion            |                                                       X                  |      1     |
      Subcutaneous Tissue, Edema           |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  57                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 5| 6| 7| 7|            |
                             DAY ON TEST   | 2| 0| 3| 3| 3| 2| 7| 3| 3| 2| 3| 2| 3| 2| 3| 2| 3| 3| 2| 2| 2| 5| 0| 3| 3|            |
                                           | 3| 5| 0| 1| 1| 9| 3| 0| 0| 9| 0| 9| 0| 9| 0| 9| 0| 0| 9| 9| 9| 9| 5| 1| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 MUSCULOSKELETAL SYSTEM - cont             |                                                                          |            |
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Peripheral Nerve                        |    M     +                                                               |   2        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |    +     +                 +                                             |   4        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Congestion                           |                                                                          |      1  2.0|
      Hyperplasia, Histiocytic             |                                                                          |      1  2.0|
      Inflammation                         |                                        2                                 |      1  2.0|
      Alveolar Epithelium, Hyperplasia,    |                                                                          |            |
           Focal                           |                                  1                                1      |      4  1.5|
      Mediastinum, Foreign Body            |                                                                          |      1     |
      Mediastinum, Inflammation,           |                                                                          |            |
          Suppurative                      |                                                                          |      1  3.0|
      Mediastinum, Thrombosis              |                                                                          |      1  4.0|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation                         |                                                                          |      1  2.0|
      Nasolacrimal Duct, Inflammation      |          2                                                               |      3  2.0|
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  A  +  +  +|  45        |
      Cornea, Inflammation                 |                      2                                                   |      2  2.0|
      Cornea, Necrosis, Focal              |             2                                                            |      1  2.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  58                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 5| 6| 7| 7|            |
                             DAY ON TEST   | 2| 0| 3| 3| 3| 2| 7| 3| 3| 2| 3| 2| 3| 2| 3| 2| 3| 3| 2| 2| 2| 5| 0| 3| 3|            |
                                           | 3| 5| 0| 1| 1| 9| 3| 0| 0| 9| 0| 9| 0| 9| 0| 9| 0| 0| 9| 9| 9| 9| 5| 1| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      A     |
    0 MG/KG                                | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
                                           | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 SPECIAL SENSES SYSTEM - cont              |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation                         |       3                                                                  |      1  3.0|
      Epithelium, Hyperplasia              |                         2                                         2      |      2  2.0|
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Hydronephrosis                       | 3                                      3                                 |      2  3.0|
      Metaplasia, Focal, Osseous           |                            1                                             |      3  1.3|
      Nephropathy                          |    2  1  2  1  1  1  1  2  1  1  1        1  2     2  2  1  2  1     2  1|     40  1.5|
      Renal Tubule, Cyst                   |                                                 X        X               |      4     |
      Renal Tubule, Hyperplasia, Focal     |                                                                          |      3  1.3|
                                           |__________________________________________________________________________|____________|
   Urethra                                 |                                        +                                 |   1        |
      Inflammation, Chronic                |                                        3                                 |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Transitional Epithelium, Hyperplasia |                                                                   3      |      1  3.0|
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  59                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7|             
                             DAY ON TEST   | 3| 9| 3| 5| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 1| 2| 3| 2| 3| 7| 3|             
                                           | 1| 4| 2| 2| 1| 1| 1| 9| 1| 1| 0| 0| 0| 2| 7| 0| 0| 1| 7| 9| 0| 9| 1| 0| 2|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    2.5                                    | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |                                                                          |             
      Inflammation                         |                                                                          |             
      Perforation                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Gallbladder                             | +  A  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Rectum                 | +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Cecum                  | +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
      Edema                                |                                                             3            |             
                                           |__________________________________________________________________________|             
   Intestine Small, Duodenum               | +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
      Epithelium, Cyst                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Intestine Small, Jejunum                | +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
      Peyer's Patch, Hyperplasia, Lymphoid |                               2                                          |             
                                           |__________________________________________________________________________|             
   Intestine Small, Ileum                  | +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     |                   X                                                      |             
      Clear Cell Focus                     | X     X        X     X     X     X     X           X        X  X        X|             
      Cyst                                 |                                                                          |             
      Eosinophilic Focus                   |             X                                                            |             
      Hematopoietic Cell Proliferation     |                         3                                                |             
      Hemorrhage                           |                                                                          |             
      Hepatodiaphragmatic Nodule           |                                                                          |             
      Infiltration Cellular, Mixed Cell    |                                        1                                 |             
      Mixed Cell Focus                     | X                                                                        |             
      Thrombosis                           |    3                                                                     |             
      Bile Duct, Hyperplasia               |                                                                          |             
      Hepatocyte, Necrosis, Focal          |    3                                                                     |             
      Hepatocyte, Vacuolization Cytoplasmic| 3     3        2  3  3     3     2     3        3  3           3         |             
                                           |__________________________________________________________________________|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  60                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7|             
                             DAY ON TEST   | 3| 9| 3| 5| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 1| 2| 3| 2| 3| 7| 3|             
                                           | 1| 4| 2| 2| 1| 1| 1| 9| 1| 1| 0| 0| 0| 2| 7| 0| 0| 1| 7| 9| 0| 9| 1| 0| 2|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    2.5                                    | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM - cont                  |                                                                          |             
   Mesentery                               |          +     +        +     +                       +     +            |             
      Inflammation                         |          3                                                               |             
      Fat, Necrosis, Focal                 |                2              1                       3                  |             
                                           |__________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acinus, Atrophy, Focal               |                                                                          |             
      Acinus, Cytoplasmic Alteration       |                                                                      2   |             
                                           |__________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Ulcer                                |    2                                                        2            |             
      Epithelium, Hyperplasia              |                                                             2            |             
                                           |__________________________________________________________________________|             
   Stomach, Glandular                      | +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
      Erosion                              |                                                                      2   |             
                                           |__________________________________________________________________________|             
   Tooth                                   | +     +        +  +  +  +  +  +  +  +  +        +  +     +  +     +     +|             
      Dysplasia                            | 4     1        1  1  3  4  2  2  1  2  2        3  2     4  1     2     3|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Infiltration Cellular, Mixed Cell    |                         2                                                |             
      Artery, Inflammation, Chronic        |                                                                   2      |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hypertrophy, Focal                   |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |                      1                                                   |             
                                           |__________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |    2  1                                   1  2     2                     |             
                                           |__________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  61                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7|             
                             DAY ON TEST   | 3| 9| 3| 5| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 1| 2| 3| 2| 3| 7| 3|             
                                           | 1| 4| 2| 2| 1| 1| 1| 9| 1| 1| 0| 0| 0| 2| 7| 0| 0| 1| 7| 9| 0| 9| 1| 0| 2|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    2.5                                    | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
      Cyst                                 |                                                          X        X      |             
                                           |__________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
      Pars Distalis, Hyperplasia, Focal    |                                                 2                        |             
                                           |__________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Follicle, Degeneration, Cystic, Focal|       1        3        2     1                       1     1  1         |             
      Follicular Cell, Hyperplasia, Focal  |                                              2                           |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |__________________________________________________________________________|             
   Tissue NOS                              |                                                       +                  |             
      Abdominal, Fibrosis                  |                                                       3                  |             
      Abdominal, Inflammation              |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration, Cystic                 |             3                 3  3                          3            |             
      Inflammation, Chronic                |                                                    3                     |             
                                           |__________________________________________________________________________|             
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                         3                                                |             
      Epithelium, Hyperplasia              |                         3                                                |             
                                           |__________________________________________________________________________|             
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Dilatation                           |                                                                          |             
                                           |__________________________________________________________________________|             
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                         3                                                |             
                                           |__________________________________________________________________________|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  62                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7|             
                             DAY ON TEST   | 3| 9| 3| 5| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 1| 2| 3| 2| 3| 7| 3|             
                                           | 1| 4| 2| 2| 1| 1| 1| 9| 1| 1| 0| 0| 0| 2| 7| 0| 0| 1| 7| 9| 0| 9| 1| 0| 2|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    2.5                                    | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
   Lymph Node                              |                                                       +     +            |             
      Iliac, Hyperplasia, Lymphoid         |                                                                          |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  M  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  M|             
      Hyperplasia, Lymphoid                |          3                                                               |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Lymphoid                |             3                                                            |             
                                           |__________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hematopoietic Cell Proliferation     |          2  2           4                 3  3           2     2     3   |             
      Hyperplasia, Lymphoid                |                               3  2                                      2|             
                                           |__________________________________________________________________________|             
   Thymus                                  | +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  +  +  +  I  +  +|             
      Angiectasis                          |                3                                                         |             
      Hyperplasia, Lymphoid                |                3                                                         |             
      Epithelial Cell, Hyperplasia         |                3                                                         |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|             
                                           |__________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Subcutaneous Tissue, Edema           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Vertebra, Fracture                   |                                                                          |             
                                           |__________________________________________________________________________|             
   Skeletal Muscle                         |    +                                                                     |             
      Fibrosis, Focal                      |    1                                                                     |             
      Inflammation                         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  63                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7|             
                             DAY ON TEST   | 3| 9| 3| 5| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 1| 2| 3| 2| 3| 7| 3|             
                                           | 1| 4| 2| 2| 1| 1| 1| 9| 1| 1| 0| 0| 0| 2| 7| 0| 0| 1| 7| 9| 0| 9| 1| 0| 2|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    2.5                                    | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 NERVOUS SYSTEM - cont                     |                                                                          |             
   Peripheral Nerve                        |                                                                      +   |             
                                           |__________________________________________________________________________|             
   Spinal Cord                             |                                                                      +   |             
      Hemorrhage                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                                          |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                      2                                                   |             
                                           |__________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         | 1                                                                        |             
      Nasolacrimal Duct, Hyperplasia, Focal|                                                                          |             
                                           |__________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Infarct                              |                2                                                         |             
      Nephropathy                          | 2     2     1  2  2  1     1  3  1  2  2  1  3  1  2  1  2  1  1  2  3  2|             
      Renal Tubule, Cyst                   |                                                                          |             
      Renal Tubule, Hyperplasia, Atypical  |                                                                          |             
      Renal Tubule, Hyperplasia, Focal     |    2                                                                     |             
                                           |__________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  64                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 6| 7| 6| 7| 7|            |
                             DAY ON TEST   | 2| 7| 3| 1| 2| 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 2| 0| 2| 1| 3| 2| 2| 3|            |
                                           | 9| 6| 0| 5| 9| 2| 2| 9| 9| 1| 1| 0| 1| 0| 0| 1| 8| 9| 4| 9| 0| 1| 7| 5| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1|      A     |
    2.5                                    | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Foreign Body                         |                                                             2            |      1  2.0|
      Inflammation                         |                                                             3            |      1  3.0|
      Perforation                          |                                                             3            |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Gallbladder                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  A  +  +  A  +|  44        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  A  +|  46        |
      Edema                                |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  A  +|  46        |
      Epithelium, Cyst                     |    X                                                                     |      1     |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  A  +  +  A  +|  45        |
      Peyer's Patch, Hyperplasia, Lymphoid |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I  +  A  +  +  A  +|  45        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Basophilic Focus                     |                                                                          |      1     |
      Clear Cell Focus                     |       X                    X  X     X     X        X  X        X         |     19     |
      Cyst                                 |                                     X                                    |      1     |
      Eosinophilic Focus                   |             X                                                            |      2     |
      Hematopoietic Cell Proliferation     |                      2                                                   |      2  2.5|
      Hemorrhage                           |                                                                      3   |      1  3.0|
      Hepatodiaphragmatic Nodule           |             2                                                            |      1  2.0|
      Infiltration Cellular, Mixed Cell    |                                                                          |      1  1.0|
      Mixed Cell Focus                     |                                                                          |      1     |
      Thrombosis                           |                                                                      3   |      2  3.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  65                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 6| 7| 6| 7| 7|            |
                             DAY ON TEST   | 2| 7| 3| 1| 2| 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 2| 0| 2| 1| 3| 2| 2| 3|            |
                                           | 9| 6| 0| 5| 9| 2| 2| 9| 9| 1| 1| 0| 1| 0| 0| 1| 8| 9| 4| 9| 0| 1| 7| 5| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1|      A     |
    2.5                                    | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
      Bile Duct, Hyperplasia               |                               1                                          |      1  1.0|
      Hepatocyte, Necrosis, Focal          |                                                                      3   |      2  3.0|
      Hepatocyte, Vacuolization Cytoplasmic|       3        2           3  3     1     2                    3        2|     19  2.6|
                                           |__________________________________________________________________________|____________|
   Mesentery                               | +                 +  +     +                    +                        |  11        |
      Inflammation                         |                                                                          |      1  3.0|
      Fat, Necrosis, Focal                 | 3                 1  3     3                                             |      7  2.3|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Acinus, Atrophy, Focal               |                1                                                         |      1  1.0|
      Acinus, Cytoplasmic Alteration       |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Ulcer                                |                                                 2                        |      3  2.0|
      Epithelium, Hyperplasia              |                                                 2                        |      2  2.0|
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +|  47        |
      Erosion                              |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Tooth                                   | +  +  +     +  +  +  +  +  +  +  +     +  +  +  +  +  +        +     +  +|  37        |
      Dysplasia                            | 3  2  4     2  3  1  4  4  2  4  2     2  4  4  2  2  1        3     4  2|     37  2.5|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Infiltration Cellular, Mixed Cell    |                                                                          |      1  2.0|
      Artery, Inflammation, Chronic        |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hypertrophy, Focal                   |                            3                                             |      1  3.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  66                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 6| 7| 6| 7| 7|            |
                             DAY ON TEST   | 2| 7| 3| 1| 2| 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 2| 0| 2| 1| 3| 2| 2| 3|            |
                                           | 9| 6| 0| 5| 9| 2| 2| 9| 9| 1| 1| 0| 1| 0| 0| 1| 8| 9| 4| 9| 0| 1| 7| 5| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1|      A     |
    2.5                                    | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ENDOCRINE SYSTEM - cont                   |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia, Focal                   |                                                                          |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          | 2                 2  2  2     2  2  2     1  2  2  1                 2   |     17  1.8|
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |                                     X                                    |      3     |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Cyst                                 |                               X                                          |      1     |
      Pars Distalis, Hyperplasia, Focal    |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Follicle, Degeneration, Cystic, Focal| 1           1  1              1              1                           |     12  1.3|
      Follicular Cell, Hyperplasia, Focal  |                      2  1           1              2                     |      5  1.6|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Tissue NOS                              |                                                                +         |   2        |
      Abdominal, Fibrosis                  |                                                                3         |      2  3.0|
      Abdominal, Inflammation              |                                                                3         |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Degeneration, Cystic                 |             3  3  3     2        3  3                       3            |     11  2.9|
      Inflammation, Chronic                |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation                         |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  67                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 6| 7| 6| 7| 7|            |
                             DAY ON TEST   | 2| 7| 3| 1| 2| 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 2| 0| 2| 1| 3| 2| 2| 3|            |
                                           | 9| 6| 0| 5| 9| 2| 2| 9| 9| 1| 1| 0| 1| 0| 0| 1| 8| 9| 4| 9| 0| 1| 7| 5| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1|      A     |
    2.5                                    | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
      Epithelium, Hyperplasia              |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Dilatation                           |                                           3        3                     |      2  3.0|
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                   3                                         3            |      3  3.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node                              |    +                                                                     |   3        |
      Iliac, Hyperplasia, Lymphoid         |    2                                                                     |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  +  +  +  +  +|  46        |
      Hyperplasia, Lymphoid                |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia, Lymphoid                |             3                                                            |      2  3.0|
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hematopoietic Cell Proliferation     | 3  3           3  2  2                 3        3                 3      |     16  2.7|
      Hyperplasia, Lymphoid                |                                     2                    2              2|      6  2.2|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
      Angiectasis                          |                                                                          |      1  3.0|
      Hyperplasia, Lymphoid                |                                                                          |      1  3.0|
      Epithelial Cell, Hyperplasia         |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  68                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 6| 7| 6| 7| 7|            |
                             DAY ON TEST   | 2| 7| 3| 1| 2| 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 2| 0| 2| 1| 3| 2| 2| 3|            |
                                           | 9| 6| 0| 5| 9| 2| 2| 9| 9| 1| 1| 0| 1| 0| 0| 1| 8| 9| 4| 9| 0| 1| 7| 5| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1|      A     |
    2.5                                    | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 INTEGUMENTARY SYSTEM - cont               |                                                                          |            |
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Subcutaneous Tissue, Edema           | 3                                                                        |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Vertebra, Fracture                   |          3                                                               |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         |    +                                            +           +            |   4        |
      Fibrosis, Focal                      |                                                                          |      1  1.0|
      Inflammation                         |                                                             2            |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Peripheral Nerve                        |    +                                                           +         |   3        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |    +     +                                                     +         |   4        |
      Hemorrhage                           |    3     3                                                               |      2  3.0|
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Congestion                           |                                                             3            |      1  3.0|
      Alveolar Epithelium, Hyperplasia,    |                                                                          |            |
           Focal                           |                1                                               2         |      3  1.7|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation                         |                         2                       1                        |      3  1.3|
      Nasolacrimal Duct, Hyperplasia, Focal|                                                                3         |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                             Page  69                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 6| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 6| 7| 6| 7| 7|            |
                             DAY ON TEST   | 2| 7| 3| 1| 2| 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 2| 0| 2| 1| 3| 2| 2| 3|            |
                                           | 9| 6| 0| 5| 9| 2| 2| 9| 9| 1| 1| 0| 1| 0| 0| 1| 8| 9| 4| 9| 0| 1| 7| 5| 0|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1|      A     |
    2.5                                    | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +|  49        |
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +|  49        |
      Infarct                              |                                                                          |      1  2.0|
      Nephropathy                          | 3  1  2     1  2  2  1  1  1  1  1  2  2  2  2     1  2  1  3  2  3     1|     44  1.7|
      Renal Tubule, Cyst                   |       X                                      X                           |      2     |
      Renal Tubule, Hyperplasia, Atypical  |       2                                                                  |      1  2.0|
      Renal Tubule, Hyperplasia, Focal     |                                              3                           |      2  2.5|
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  70                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 2| 7| 6| 7| 6| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 6| 2| 2| 2| 0| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3|             
                                           | 1| 1| 9| 7| 9| 5| 1| 0| 0| 0| 1| 5| 1| 1| 0| 0| 1| 0| 1| 7| 0| 1| 1| 0| 1|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    12.5                                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Gallbladder                             | +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Cecum                  | +  A  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  A  +  +  +  +  +|             
      Edema                                |                                                                          |             
                                           |__________________________________________________________________________|             
   Intestine Small, Duodenum               | +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Jejunum                | +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Ileum                  | +  A  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  A  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Clear Cell Focus                     |             X     X     X  X        X     X     X     X        X  X     X|             
      Eosinophilic Focus                   | X                                                                    X   |             
      Infiltration Cellular, Mixed Cell    |                                                                          |             
      Thrombosis                           |                                                                          |             
      Hepatocyte, Necrosis, Focal          |                                                                          |             
      Hepatocyte, Vacuolization Cytoplasmic| 2     3  2  3  2  2     3  2  1     2     3     3  2  2     2  3  3  2  3|             
      Hepatocyte, Centrilobular, Necrosis  |                                                                          |             
                                           |__________________________________________________________________________|             
   Mesentery                               |             +                    +                                       |             
      Fat, Necrosis, Focal                 |             1                                                            |             
                                           |__________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acinus, Atrophy, Focal               |                            2     2                                       |             
      Acinus, Inflammation                 |                                                                2         |             
      Duct, Cyst                           |                                                                          |             
                                           |__________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  71                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 2| 7| 6| 7| 6| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 6| 2| 2| 2| 0| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3|             
                                           | 1| 1| 9| 7| 9| 5| 1| 0| 0| 0| 1| 5| 1| 1| 0| 0| 1| 0| 1| 7| 0| 1| 1| 0| 1|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    12.5                                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM - cont                  |                                                                          |             
      Epithelium, Hyperplasia              |                1                                      2                  |             
                                           |__________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  A  +  +  +  +  +|             
      Erosion                              |          1                                                               |             
                                           |__________________________________________________________________________|             
   Tooth                                   | +        +  +  +  +     +        +  +     +  +     +  +     +  +  +  +  +|             
      Dysplasia                            | 3        1  2  2  2     2        2  3     4  2     4  3     1  1  2  2  3|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Infiltration Cellular, Mixed Cell    |                                                                2         |             
      Mineralization, Focal                |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Accessory Adrenal Cortical Nodule    |                                                                          |             
      Cytoplasmic Alteration, Focal        |                                     2                                    |             
      Degeneration, Cystic                 |                         2                                                |             
                                           |__________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |                                                                          |             
                                           |__________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                            2                          2                 2|             
                                           |__________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                X              X                                          |             
                                           |__________________________________________________________________________|             
   Pituitary Gland                         | I  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |             X                                                            |             
                                           |__________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Follicle, Degeneration, Cystic, Focal| 2           1     2                    1  2  1     1              2      |             
      Follicular Cell, Hyperplasia, Focal  |                      3                          2     1              2  1|             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  72                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 2| 7| 6| 7| 6| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 6| 2| 2| 2| 0| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3|             
                                           | 1| 1| 9| 7| 9| 5| 1| 0| 0| 0| 1| 5| 1| 1| 0| 0| 1| 0| 1| 7| 0| 1| 1| 0| 1|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    12.5                                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 GENERAL BODY SYSTEM - cont                |                                                                          |             
                                           |__________________________________________________________________________|             
   Peritoneum                              |                                  +                                       |             
                                           |__________________________________________________________________________|             
   Tissue NOS                              |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration, Cystic                 |             3  3                          3  3  3     3     3           3|             
      Inflammation, Chronic                | 3                                                                        |             
                                           |__________________________________________________________________________|             
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Epithelium, Hyperplasia              |       2                                                                  |             
                                           |__________________________________________________________________________|             
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Epithelium, Hyperplasia              |                                                                          |             
                                           |__________________________________________________________________________|             
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          | 3                                                        3               |             
                                           |__________________________________________________________________________|             
   Lymph Node                              |                                  +                 +                     |             
      Mediastinal, Hemorrhage              |                                                                          |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                                          |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  +  +  +|             
      Hemorrhage                           |                                                          3               |             
      Hyperplasia, Lymphoid                |                                        3                    3            |             
                                           |__________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  73                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 2| 7| 6| 7| 6| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 6| 2| 2| 2| 0| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3|             
                                           | 1| 1| 9| 7| 9| 5| 1| 0| 0| 0| 1| 5| 1| 1| 0| 0| 1| 0| 1| 7| 0| 1| 1| 0| 1|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    12.5                                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
      Atrophy                              |    3                                                                     |             
      Hematopoietic Cell Proliferation     | 3        3     3  2  3     2  2  4     3     3     2        2            |             
      Hyperplasia, Lymphoid                |                                                                      2   |             
      Lymphoid Follicle, Necrosis          |          2                                                               |             
                                           |__________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  M  +  +  M  M  +  I  +  +  M  +  +  +  +  I  I  +  +|             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|             
                                           |__________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Subcutaneous Tissue, Edema           |                3                                                         |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Skeletal Muscle                         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Compression, Focal                   |                                                                          |             
                                           |__________________________________________________________________________|             
   Peripheral Nerve                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Spinal Cord                             |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                                2         |             
      Hyperplasia, Histiocytic             |                                              4                       3   |             
      Thrombosis                           |                      2                                                   |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                                                    1                     |             
                                           |__________________________________________________________________________|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  74                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 2| 7| 6| 7| 6| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 3| 6| 2| 2| 2| 0| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3|             
                                           | 1| 1| 9| 7| 9| 5| 1| 0| 0| 0| 1| 5| 1| 1| 0| 0| 1| 0| 1| 7| 0| 1| 1| 0| 1|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    12.5                                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |    2                                                                     |             
      Nasolacrimal Duct, Inflammation      |                                                                          |             
                                           |__________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |                                                                          |             
                                           |__________________________________________________________________________|             
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Epithelium, Hyperplasia              |                                        2                                 |             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Lymphoid                |                                                                          |             
      Metaplasia, Focal, Osseous           |       1        1                                                         |             
      Mineralization, Focal                |                                                                          |             
      Nephropathy                          | 1     1  2  2  2  2  1  2  1  1     1  3  2  2  1  2  1     1  1  1  2  2|             
      Artery, Inflammation, Chronic        |                                                                          |             
      Renal Tubule, Accumulation, Hyaline  |                                                                          |             
          Droplet                          |                                                                          |             
      Renal Tubule, Cyst                   | X                                                                 X      |             
      Renal Tubule, Hyperplasia, Focal     |                   1                                                      |             
      Renal Tubule, Pigmentation           |                                                                          |             
                                           |__________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  75                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 6|            |
                             DAY ON TEST   | 2| 3| 7| 3| 2| 3| 3| 3| 3| 0| 9| 3| 3| 3| 3| 2| 3| 3| 2| 9| 8| 4| 3| 3| 3|            |
                                           | 9| 0| 3| 1| 9| 1| 1| 0| 1| 5| 0| 1| 1| 1| 1| 9| 1| 0| 9| 6| 0| 5| 0| 1| 8|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|      A     |
    12.5                                   | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Gallbladder                             | +  +  A  +  +  +  +  +  +  +  I  +  +  +  M  +  +  +  +  +  M  +  +  +  +|  45        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  46        |
      Edema                                |                                                                3         |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  A  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  46        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  A  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +|  44        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Clear Cell Focus                     |    X     X        X     X              X  X  X  X  X  X           X      |     22     |
      Eosinophilic Focus                   |                                                                          |      2     |
      Infiltration Cellular, Mixed Cell    |                      1              1                                    |      2  1.0|
      Thrombosis                           |                                                          3               |      1  3.0|
      Hepatocyte, Necrosis, Focal          |                                                          3     3         |      2  3.0|
      Hepatocyte, Vacuolization Cytoplasmic|          3     2  3              2  3  2  3  2  3  3  3        3  2      |     32  2.5|
      Hepatocyte, Centrilobular, Necrosis  |                               3                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Mesentery                               |                         +                                      +  +      |   5        |
      Fat, Necrosis, Focal                 |                         3                                      3  3      |      4  2.5|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Acinus, Atrophy, Focal               |                                                                          |      2  2.0|
      Acinus, Inflammation                 |                                                                          |      1  2.0|
      Duct, Cyst                           |                                     X                                    |      1     |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  76                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 6|            |
                             DAY ON TEST   | 2| 3| 7| 3| 2| 3| 3| 3| 3| 0| 9| 3| 3| 3| 3| 2| 3| 3| 2| 9| 8| 4| 3| 3| 3|            |
                                           | 9| 0| 3| 1| 9| 1| 1| 0| 1| 5| 0| 1| 1| 1| 1| 9| 1| 0| 9| 6| 0| 5| 0| 1| 8|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|      A     |
    12.5                                   | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Epithelium, Hyperplasia              |                      2                                                   |      3  1.7|
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
      Erosion                              |                                                                3         |      2  2.0|
                                           |__________________________________________________________________________|____________|
   Tooth                                   |       +  +  +  +  +  +  +     +  +     +  +  +  +     +  +        +      |  33        |
      Dysplasia                            |       2  4  4  2  1  1  3     4  2     2  3  2  2     3  2        4      |     33  2.4|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Infiltration Cellular, Mixed Cell    |                                                                          |      1  2.0|
      Mineralization, Focal                |                                                                3         |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Accessory Adrenal Cortical Nodule    |                               1                                          |      1  1.0|
      Cytoplasmic Alteration, Focal        |                         1           2           2  1                     |      5  1.6|
      Degeneration, Cystic                 |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia, Focal                   |                                        2                                 |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Hyperplasia                          |                   1     2        1              1                        |      7  1.6|
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Cyst                                 |                                                                      X   |      3     |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  77                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 6|            |
                             DAY ON TEST   | 2| 3| 7| 3| 2| 3| 3| 3| 3| 0| 9| 3| 3| 3| 3| 2| 3| 3| 2| 9| 8| 4| 3| 3| 3|            |
                                           | 9| 0| 3| 1| 9| 1| 1| 0| 1| 5| 0| 1| 1| 1| 1| 9| 1| 0| 9| 6| 0| 5| 0| 1| 8|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|      A     |
    12.5                                   | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ENDOCRINE SYSTEM - cont                   |                                                                          |            |
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Cyst                                 |                                                                          |      1     |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Follicle, Degeneration, Cystic, Focal| 2                                                     1  1               |     11  1.5|
      Follicular Cell, Hyperplasia, Focal  |          3  1                       1     2  1     2                     |     11  1.7|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Peritoneum                              |                                                                          |   1        |
                                           |__________________________________________________________________________|____________|
   Tissue NOS                              |                            M                                             |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Degeneration, Cystic                 |    3                 3                       3        3     3  3  3  2   |     16  2.9|
      Inflammation, Chronic                |       3                                                                  |      2  3.0|
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Epithelium, Hyperplasia              |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Epithelium, Hyperplasia              |          1                                                               |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Atrophy                              |                                                             3            |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |                      3                                                   |      3  3.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  78                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 6|            |
                             DAY ON TEST   | 2| 3| 7| 3| 2| 3| 3| 3| 3| 0| 9| 3| 3| 3| 3| 2| 3| 3| 2| 9| 8| 4| 3| 3| 3|            |
                                           | 9| 0| 3| 1| 9| 1| 1| 0| 1| 5| 0| 1| 1| 1| 1| 9| 1| 0| 9| 6| 0| 5| 0| 1| 8|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|      A     |
    12.5                                   | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lymph Node                              |                                                                +         |   3        |
      Mediastinal, Hemorrhage              |                                                                3         |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +|  48        |
      Hemorrhage                           |                                                                3         |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Hemorrhage                           |                                                             3  3         |      3  3.0|
      Hyperplasia, Lymphoid                |                                                                          |      2  3.0|
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Atrophy                              |                                                                          |      1  3.0|
      Hematopoietic Cell Proliferation     | 3  3           3     3     3  3  2           3     3     3  3  3     3   |     25  2.8|
      Hyperplasia, Lymphoid                |                                                                      2   |      2  2.0|
      Lymphoid Follicle, Necrosis          |                                                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  I  +  +  +  A  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +|  40        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|            |
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Subcutaneous Tissue, Edema           |                                                                3         |      2  3.0|
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         |                +                                                         |   1        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  79                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 6|            |
                             DAY ON TEST   | 2| 3| 7| 3| 2| 3| 3| 3| 3| 0| 9| 3| 3| 3| 3| 2| 3| 3| 2| 9| 8| 4| 3| 3| 3|            |
                                           | 9| 0| 3| 1| 9| 1| 1| 0| 1| 5| 0| 1| 1| 1| 1| 9| 1| 0| 9| 6| 0| 5| 0| 1| 8|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|      A     |
    12.5                                   | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 NERVOUS SYSTEM - cont                     |                                                                          |            |
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Compression, Focal                   |                            3                                             |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Peripheral Nerve                        |                                                             +            |   1        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |                                                             +            |   1        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hemorrhage                           |                                                                          |      1  2.0|
      Hyperplasia, Histiocytic             |                                                          2               |      3  3.0|
      Thrombosis                           |                                                                          |      1  2.0|
      Alveolar Epithelium, Hyperplasia,    |                                                                          |            |
           Focal                           |                                                          1               |      2  1.0|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation                         |                                                             2  3         |      3  2.3|
      Nasolacrimal Duct, Inflammation      |                                              2                           |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Atrophy                              |                            4                                             |      1  4.0|
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Epithelium, Hyperplasia              |                                                                      2   |      2  2.0|
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia, Lymphoid                |                                     3                                    |      1  3.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  80                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 6|            |
                             DAY ON TEST   | 2| 3| 7| 3| 2| 3| 3| 3| 3| 0| 9| 3| 3| 3| 3| 2| 3| 3| 2| 9| 8| 4| 3| 3| 3|            |
                                           | 9| 0| 3| 1| 9| 1| 1| 0| 1| 5| 0| 1| 1| 1| 1| 9| 1| 0| 9| 6| 0| 5| 0| 1| 8|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|      A     |
    12.5                                   | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 URINARY SYSTEM - cont                     |                                                                          |            |
      Metaplasia, Focal, Osseous           |       1                                                                  |      3  1.0|
      Mineralization, Focal                |                                                                2         |      1  2.0|
      Nephropathy                          | 2  1     2  2  2  1  3  2  2     2  1  1  1  2  1  2  1     3  2  1  2  4|     44  1.7|
      Artery, Inflammation, Chronic        |                                           2                              |      1  2.0|
      Renal Tubule, Accumulation, Hyaline  |                                                                          |            |
          Droplet                          |                               2                                          |      1  2.0|
      Renal Tubule, Cyst                   | X                                                                        |      3     |
      Renal Tubule, Hyperplasia, Focal     |                                                                          |      1  1.0|
      Renal Tubule, Pigmentation           |                               2                                          |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  81                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7|             
                             DAY ON TEST   | 2| 3| 1| 8| 4| 3| 2| 2| 2| 2| 2| 2| 0| 3| 3| 3| 2| 2| 3| 2| 3| 3| 9| 3| 2|             
                                           | 9| 1| 0| 7| 1| 0| 9| 9| 9| 9| 9| 9| 6| 1| 0| 0| 9| 9| 1| 9| 1| 0| 0| 0| 9|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    25                                     | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |                                                                          |             
      Inflammation                         |       3                                                                  |             
      Perforation                          |       3                                                                  |             
                                           |__________________________________________________________________________|             
   Gallbladder                             | +  +  A  A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  M  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic, Focal         |                                                                          |             
                                           |__________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Liver                                   | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Clear Cell Focus                     |       X        X  X  X  X        X     X  X  X                           |             
      Congestion                           |                                                                          |             
      Cyst                                 |                                                             X            |             
      Eosinophilic Focus                   |                                                       X                  |             
      Hepatodiaphragmatic Nodule           |                                                                          |             
      Infiltration Cellular, Mixed Cell    |                               1                    2                    1|             
      Thrombosis                           |                                                                          |             
      Hepatocyte, Necrosis, Focal          |             1                                                            |             
      Hepatocyte, Syncytial Alteration,    |                                                                          |             
           Focal                           |                                  1                                       |             
      Hepatocyte, Vacuolization Cytoplasmic|       2           2  2  2        2     3  2  2        3  2           3  2|             
                                           |__________________________________________________________________________|             
   Mesentery                               |                         +                          +              +      |             
      Inflammation                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Pancreas                                | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  82                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7|             
                             DAY ON TEST   | 2| 3| 1| 8| 4| 3| 2| 2| 2| 2| 2| 2| 0| 3| 3| 3| 2| 2| 3| 2| 3| 3| 9| 3| 2|             
                                           | 9| 1| 0| 7| 1| 0| 9| 9| 9| 9| 9| 9| 6| 1| 0| 0| 9| 9| 1| 9| 1| 0| 0| 0| 9|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    25                                     | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ALIMENTARY SYSTEM - cont                  |                                                                          |             
      Acinus, Atrophy, Focal               |                                                                          |             
                                           |__________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Ulcer                                |                                                                          |             
      Epithelium, Hyperplasia              |                                                                          |             
                                           |__________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Erosion                              |                                                                          |             
                                           |__________________________________________________________________________|             
   Tooth                                   | +  +  +  +  +     +  +  +  +  +  +     +  +  +     +  +  +     +     +  +|             
      Dysplasia                            | 2  2  3  2  2     3  3  3  1  2  3     3  3  4     3  1  2     2     2  4|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Mineralization, Focal                |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cytoplasmic Alteration, Focal        |                                                                      1   |             
      Hyperplasia, Focal                   |                                                                          |             
                                           |__________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |                                                                          |             
                                           |__________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |       2        2  2     2              3  2                    2         |             
                                           |__________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  I  +  +|             
      Cyst                                 |                                              X                           |             
                                           |__________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
                                           |__________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  83                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7|             
                             DAY ON TEST   | 2| 3| 1| 8| 4| 3| 2| 2| 2| 2| 2| 2| 0| 3| 3| 3| 2| 2| 3| 2| 3| 3| 9| 3| 2|             
                                           | 9| 1| 0| 7| 1| 0| 9| 9| 9| 9| 9| 9| 6| 1| 0| 0| 9| 9| 1| 9| 1| 0| 0| 0| 9|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    25                                     | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
      Follicle, Degeneration, Cystic, Focal|                   1           1           1           1     1            |             
      Follicular Cell, Hyperplasia, Focal  |                               1                       2        1         |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |__________________________________________________________________________|             
   Tissue NOS                              |                                                                          |             
      Foreign Body                         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Coagulating Gland                       |                         +                                                |             
                                           |__________________________________________________________________________|             
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  +  +  +  I|             
      Degeneration, Cystic                 | 3           3              3                             3               |             
      Inflammation, Chronic                |                                                                          |             
                                           |__________________________________________________________________________|             
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                                                                          |             
                                           |__________________________________________________________________________|             
   Seminal Vesicle                         | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                     3                                    |             
                                           |__________________________________________________________________________|             
   Lymph Node                              |          +                    +  +                 +              +      |             
      Mediastinal, Hyperplasia, Lymphoid   |                                  3                                       |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  M|             
      Hyperplasia, Lymphoid                |                                  3                                       |             
                                           |__________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I  +  +|             
      Hematopoietic Cell Proliferation     |                                     3                                    |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  84                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7|             
                             DAY ON TEST   | 2| 3| 1| 8| 4| 3| 2| 2| 2| 2| 2| 2| 0| 3| 3| 3| 2| 2| 3| 2| 3| 3| 9| 3| 2|             
                                           | 9| 1| 0| 7| 1| 0| 9| 9| 9| 9| 9| 9| 6| 1| 0| 0| 9| 9| 1| 9| 1| 0| 0| 0| 9|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    25                                     | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
                                           |__________________________________________________________________________|             
   Spleen                                  | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Fibrosis, Focal                      |                                                                          |             
      Hematopoietic Cell Proliferation     | 3  2  2     3     3  2  2     3     3  2  2  2  3  3  2        4  3  3   |             
      Hyperplasia, Lymphoid                |                                                 2     2                  |             
                                           |__________________________________________________________________________|             
   Thymus                                  | +  +  +  A  +  +  +  +  +  I  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |__________________________________________________________________________|             
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  +  M  M  M  M  M  M  M  M  M  M  M  M|             
                                           |__________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Ulcer                                |             3                                                            |             
      Pinna, Inflammation, Focal           |                                                                          |             
      Subcutaneous Tissue, Edema           |          3                                                               |             
      Subcutaneous Tissue, Inflammation,   |                                                                          |             
           Focal, Suppurative              |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |__________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperostosis                         |             1                             2                              |             
                                           |__________________________________________________________________________|             
   Skeletal Muscle                         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                           |__________________________________________________________________________|             
   Peripheral Nerve                        |                                                                   +      |             
                                           |__________________________________________________________________________|             
   Spinal Cord                             |                                                                   +      |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |__________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |       3                                                                  |             
 __________________________________________|__________________________________________________________________________|             
                                                                                                                                    
                                                                                                                                    
                                                             Page  85                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7|             
                             DAY ON TEST   | 2| 3| 1| 8| 4| 3| 2| 2| 2| 2| 2| 2| 0| 3| 3| 3| 2| 2| 3| 2| 3| 3| 9| 3| 2|             
                                           | 9| 1| 0| 7| 1| 0| 9| 9| 9| 9| 9| 9| 6| 1| 0| 0| 9| 9| 1| 9| 1| 0| 0| 0| 9|             
 __________________________________________|__________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    25                                     | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7|             
    MG/KG                                  | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5|             
 __________________________________________|__________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
      Hemorrhage                           |                                     3                                    |             
      Hyperplasia, Histiocytic             |                                                          1               |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                                              1                       1   |             
                                           |__________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                                                          |             
      Inflammation                         |    2  1  2                                                        2      |             
      Nasolacrimal Duct, Hyperplasia, Focal|                                                                          |             
      Nasolacrimal Duct, Inflammation      |                                                                          |             
                                           |__________________________________________________________________________|             
   Trachea                                 | +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |__________________________________________________________________________|             
   Eye                                     | +  +  +  A  I  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cataract                             |                                                                          |             
      Cornea, Inflammation                 |                                                                          |             
      Retrobulbar, Inflammation, Chronic,  |                                                                          |             
           Focal                           |                                                                          |             
                                           |__________________________________________________________________________|             
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 | X                                                                        |             
      Inflammation                         |                               1                                          |             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |__________________________________________________________________________|             
   Kidney                                  | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Infarct                              |                                                       2                  |             
      Metaplasia, Focal, Osseous           |                   1                                1                     |             
      Nephropathy                          |    1        2  1  1  1     1  1  1     1  1  1  3  3  2  1  2  2  2  1  1|             
      Renal Tubule, Cyst                   |                                                                X         |             
      Renal Tubule, Hyperplasia, Focal     |                                                                          |             
                                           |__________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 __________________________________________|__________________________________________________________________________              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  86                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7|            |
                             DAY ON TEST   | 3| 3| 3| 2| 1| 3| 0| 3| 2| 3| 2| 1| 3| 3| 2| 3| 3| 2| 3| 2| 3| 3| 3| 9| 3|            |
                                           | 2| 1| 1| 9| 0| 1| 5| 0| 9| 1| 9| 6| 0| 1| 9| 1| 0| 9| 0| 9| 2| 0| 0| 4| 1|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2|      A     |
    25                                     | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Foreign Body                         |             3                                                            |      1  3.0|
      Inflammation                         |                                                                          |      1  3.0|
      Perforation                          |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Gallbladder                             | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +|  44        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
      Inflammation, Chronic, Focal         |                                                 3                        |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I  +|  46        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
      Clear Cell Focus                     |       X              X        X           X  X        X        X         |     16     |
      Congestion                           |                                                                      3   |      1  3.0|
      Cyst                                 |                                                                          |      1     |
      Eosinophilic Focus                   |       X                                                                  |      2     |
      Hepatodiaphragmatic Nodule           |                               X                                          |      1     |
      Infiltration Cellular, Mixed Cell    |                                                    2     1              1|      6  1.3|
      Thrombosis                           |                   3                                                      |      1  3.0|
      Hepatocyte, Necrosis, Focal          |                                  1                 3                 3   |      4  2.0|
      Hepatocyte, Syncytial Alteration,    |                                                                          |            |
           Focal                           |                                                                          |      1  1.0|
      Hepatocyte, Vacuolization Cytoplasmic|                3     2  3     2     2  2     3        3  1     1  2      |     23  2.2|
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  87                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7|            |
                             DAY ON TEST   | 3| 3| 3| 2| 1| 3| 0| 3| 2| 3| 2| 1| 3| 3| 2| 3| 3| 2| 3| 2| 3| 3| 3| 9| 3|            |
                                           | 2| 1| 1| 9| 0| 1| 5| 0| 9| 1| 9| 6| 0| 1| 9| 1| 0| 9| 0| 9| 2| 0| 0| 4| 1|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2|      A     |
    25                                     | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
   Mesentery                               |                                  +                                       |   4        |
      Inflammation                         |                                  2                                       |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Acinus, Atrophy, Focal               |                                     1                                    |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Ulcer                                |                   2                                                      |      1  2.0|
      Epithelium, Hyperplasia              |                   2                                                  3   |      2  2.5|
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +|  48        |
      Erosion                              |             2                                                            |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Tooth                                   |       +  +  +  +  +  +  +  +  +  +        +  +  +        +        +      |  35        |
      Dysplasia                            |       2  4  1  1  4  3  3  2  3  4        4  3  2        3        2      |     35  2.6|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Mineralization, Focal                |    1                                                                     |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  +|  48        |
      Cytoplasmic Alteration, Focal        | 2                          2        3           1                        |      5  1.8|
      Hyperplasia, Focal                   |                      2                                                   |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +|  48        |
      Hyperplasia, Focal                   |                                                       1                  |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Hyperplasia                          |          2           2        2  3  2                          1         |     13  2.1|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  88                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7|            |
                             DAY ON TEST   | 3| 3| 3| 2| 1| 3| 0| 3| 2| 3| 2| 1| 3| 3| 2| 3| 3| 2| 3| 2| 3| 3| 3| 9| 3|            |
                                           | 2| 1| 1| 9| 0| 1| 5| 0| 9| 1| 9| 6| 0| 1| 9| 1| 0| 9| 0| 9| 2| 0| 0| 4| 1|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2|      A     |
    25                                     | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 ENDOCRINE SYSTEM - cont                   |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  46        |
      Cyst                                 |                               X                                          |      2     |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |                X           X                                X            |      3     |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Follicle, Degeneration, Cystic, Focal|                                  2     2                                 |      7  1.3|
      Follicular Cell, Hyperplasia, Focal  |    2                                2  2           1     2        2  1  1|     11  1.5|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Tissue NOS                              |             +                                                            |   1        |
      Foreign Body                         |             3                                                            |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Coagulating Gland                       |                                                                          |   1        |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +|  49        |
                                           |__________________________________________________________________________|____________|
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Degeneration, Cystic                 |                                     3  3     3  3        3  3        3   |     11  3.0|
      Inflammation, Chronic                |          3                       3                 3                     |      3  3.0|
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Inflammation                         |                                  2                                       |      1  2.0|
                                           |__________________________________________________________________________|____________|
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  89                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7|            |
                             DAY ON TEST   | 3| 3| 3| 2| 1| 3| 0| 3| 2| 3| 2| 1| 3| 3| 2| 3| 3| 2| 3| 2| 3| 3| 3| 9| 3|            |
                                           | 2| 1| 1| 9| 0| 1| 5| 0| 9| 1| 9| 6| 0| 1| 9| 1| 0| 9| 0| 9| 2| 0| 0| 4| 1|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2|      A     |
    25                                     | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |       3                                                                  |      2  3.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node                              |                                                    +                     |   6        |
      Mediastinal, Hyperplasia, Lymphoid   |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
      Hyperplasia, Lymphoid                |       3                                                                  |      2  3.0|
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Hematopoietic Cell Proliferation     |                                                                          |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Fibrosis, Focal                      |                2                                                         |      1  2.0|
      Hematopoietic Cell Proliferation     |                2     2     2           2  2  2  2  3     3  3           3|     29  2.5|
      Hyperplasia, Lymphoid                |                                           2                              |      3  2.0|
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  I  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I  +  +  +  I|  44        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M  M|   1        |
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Ulcer                                |                                                                         3|      2  3.0|
      Pinna, Inflammation, Focal           |                                                                         2|      1  2.0|
      Subcutaneous Tissue, Edema           |                                                                          |      1  3.0|
      Subcutaneous Tissue, Inflammation,   |                                                                          |            |
           Focal, Suppurative              |       3                                                                  |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  90                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7|            |
                             DAY ON TEST   | 3| 3| 3| 2| 1| 3| 0| 3| 2| 3| 2| 1| 3| 3| 2| 3| 3| 2| 3| 2| 3| 3| 3| 9| 3|            |
                                           | 2| 1| 1| 9| 0| 1| 5| 0| 9| 1| 9| 6| 0| 1| 9| 1| 0| 9| 0| 9| 2| 0| 0| 4| 1|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2|      A     |
    25                                     | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 MUSCULOSKELETAL SYSTEM - cont             |                                                                          |            |
      Hyperostosis                         |                                                                          |      2  1.5|
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         |                   +                                                      |   1        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
                                           |__________________________________________________________________________|____________|
   Peripheral Nerve                        |       +                                                                  |   2        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             |       +                                                                  |   2        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Congestion                           |                                                                          |      1  3.0|
      Hemorrhage                           |                                                                          |      1  3.0|
      Hyperplasia, Histiocytic             |                                        2                                 |      2  1.5|
      Alveolar Epithelium, Hyperplasia,    |                                                                          |            |
           Focal                           |    2  1                                                                  |      4  1.3|
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Hyperplasia                          |          3                                                               |      1  3.0|
      Inflammation                         |       2                                   2                              |      6  1.8|
      Nasolacrimal Duct, Hyperplasia, Focal|       2                                                                  |      1  2.0|
      Nasolacrimal Duct, Inflammation      |                         1                                                |      1  1.0|
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  49        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  47        |
      Cataract                             |       2                                                                  |      1  2.0|
      Cornea, Inflammation                 |       2                                                                  |      1  2.0|
 _____________________________________________________________________________________________________________________|____________|
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                             Page  91                                                               
                                                                                                                                   
NTP Experiment-Test: 91005-08                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09  
Study Type: CHRONIC                                     METHYLENE BLUE TRIHYDRATE                                 Date: 06/02/05    
Route: GAVAGE                                                                                                     Time: 13:15:34    
 __________________________________________________________________________________________________________________________________ 
                                           | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7|            |
                             DAY ON TEST   | 3| 3| 3| 2| 1| 3| 0| 3| 2| 3| 2| 1| 3| 3| 2| 3| 3| 2| 3| 2| 3| 3| 3| 9| 3|            |
                                           | 2| 1| 1| 9| 0| 1| 5| 0| 9| 1| 9| 6| 0| 1| 9| 1| 0| 9| 0| 9| 2| 0| 0| 4| 1|            |
 __________________________________________|__________________________________________________________________________|      T (*) |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      O     |
   B6C3F1 MICE MALE                        | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2|      A     |
    25                                     | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0|      L     |
    MG/KG                                  | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0|            |
 _____________________________________________________________________________________________________________________|____________|
 SPECIAL SENSES SYSTEM - cont              |                                                                          |            |
      Retrobulbar, Inflammation, Chronic,  |                                                                          |            |
           Focal                           |       3                                                                  |      1  3.0|
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  50        |
      Cyst                                 |                                                                          |      1     |
      Inflammation                         |                                                                          |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
      Infarct                              |                                                                          |      1  2.0|
      Metaplasia, Focal, Osseous           |                               2              1                           |      4  1.3|
      Nephropathy                          | 1  1  2  1     1  2  2  1  1     3  1  1  1  1  2  1  2  2  1  1  1  2  3|     43  1.5|
      Renal Tubule, Cyst                   |                                                       X                  |      2     |
      Renal Tubule, Hyperplasia, Focal     |                                        2                       1         |      2  1.5|
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|  48        |
 __________________________________________________________________________________________________________________________________ 
                                                                                                                                    
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  92                                                               
                                                                                                                                   
                             ------------------------------------------------------------                                           
                             ----------              END OF REPORT             ----------                                           
                             ------------------------------------------------------------