TDMS Study 99034-01 Pathology Tables
NTP Experiment-Test: 99034-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:49:58
FINAL #1 MICE
Facility: Battelle Columbus Laboratory
Chemical CAS #: 71-43-2
Lock Date: 10/15/01
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
a Number of animals examined microscopically at site and number of animals with lesion
Page 1
NTP Experiment-Test: 99034-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:49:58
____________________________________________________________________________________________________________________________________
P16(INK4A)/(+/-) (C57BL/6) FEMALE 0 MG/KG 25 MG/KG 50 MG/KG 100 200
MG/KG MG/KG
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 15 15 15 15 15
Early Deaths
Survivors
Terminal Sacrifice 15 15 15 15 15
Animals Examined Microscopically 15 15 15 15 15
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Intestine Small, Duodenum (15) (15) (15) (15) (15)
Inflammation, Chronic Active 2 (13%)
Liver (15) (15) (15) (15) (15)
Hematopoietic Cell Proliferation 1 (7%) 1 (7%)
Inflammation, Chronic 10 (67%) 6 (40%) 6 (40%) 4 (27%) 11 (73%)
Stomach, Forestomach (15) (15) (15) (15) (15)
Erosion 1 (7%)
Hyperplasia 1 (7%)
Inflammation, Chronic 3 (20%)
Stomach, Glandular (15) (15) (15) (15) (15)
Mineralization 1 (7%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (15) (15) (15) (15) (15)
Myocardium, Inflammation, Chronic 1 (7%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Pituitary Gland (15) (15) (15) (15) (15)
Infiltration Cellular, Lymphoid 1 (7%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
a Number of animals examined microscopically at site and number of animals with lesion
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NTP Experiment-Test: 99034-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:49:58
____________________________________________________________________________________________________________________________________
P16(INK4A)/(+/-) (C57BL/6) FEMALE 0 MG/KG 25 MG/KG 50 MG/KG 100 200
MG/KG MG/KG
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM - CONT
Clitoral Gland (1)
Pigmentation 1 (100%)
Ovary (14) (15) (15) (15) (15)
Angiectasis 1 (7%)
Cyst 1 (7%) 2 (13%) 2 (13%)
Uterus (15) (15) (15) (15) (15)
Inflammation, Chronic Active 1 (7%)
Inflammation, Suppurative 1 (7%) 1 (7%) 1 (7%)
Endometrium, Hyperplasia, Cystic 12 (80%) 15 (100%) 15 (100%) 15 (100%) 14 (93%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Lymph Node (13) (11) (11) (12) (13)
Lumbar, Hyperplasia, Lymphoid 1 (8%)
Lumbar, Pigmentation 1 (8%)
Mediastinal, Atrophy 2 (18%) 4 (33%) 2 (15%)
Lymph Node, Mandibular (14) (15) (15) (15) (15)
Atrophy 1 (7%) 2 (13%) 4 (27%)
Hyperplasia, Lymphoid 1 (7%) 4 (27%) 2 (13%) 5 (33%) 3 (20%)
Infiltration Cellular, Polymorphonuclear 1 (7%) 2 (13%)
Lymph Node, Mesenteric (15) (15) (15) (15) (15)
Atrophy 2 (13%) 3 (20%) 8 (53%) 6 (40%)
Hyperplasia, Lymphoid 1 (7%)
Spleen (15) (15) (15) (15) (15)
Hematopoietic Cell Proliferation 5 (33%) 5 (33%) 2 (13%) 3 (20%) 7 (47%)
Infiltration Cellular, Lymphocyte 1 (7%)
Lymphoid Follicle, Atrophy 1 (7%) 1 (7%) 1 (7%)
Thymus (15) (15) (15) (15) (15)
Hyperplasia, Atypical 1 (7%) 1 (7%) 1 (7%) 1 (7%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (15) (15) (15) (15) (15)
Hyperplasia 2 (13%)
Skin (15) (15) (15) (15) (15)
Inflammation, Granulomatous 1 (7%) 1 (7%)
Inflammation, Suppurative 1 (7%) 1 (7%)
Pigmentation 1 (7%) 8 (53%) 15 (100%) 15 (100%)
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 3
NTP Experiment-Test: 99034-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:49:58
____________________________________________________________________________________________________________________________________
P16(INK4A)/(+/-) (C57BL/6) FEMALE 0 MG/KG 25 MG/KG 50 MG/KG 100 200
MG/KG MG/KG
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (15) (15) (15) (15) (15)
Fibrosis 1 (7%) 1 (7%) 1 (7%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
None
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (15) (15) (15) (15) (15)
Inflammation, Chronic Active 2 (13%) 2 (13%) 2 (13%)
Alveolar Epithelium, Hyperplasia, Focal 1 (7%) 3 (20%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
None
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (15) (15) (15) (15) (15)
Casts Protein 5 (33%) 4 (27%) 5 (33%) 8 (53%) 7 (47%)
Infiltration Cellular, Lymphoid 1 (7%)
Infiltration Cellular, Lymphocyte 1 (7%)
Nephropathy 3 (20%) 1 (7%) 2 (13%) 1 (7%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 4
NTP Experiment-Test: 99034-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:49:58
____________________________________________________________________________________________________________________________________
P16(INK4A)/(+/-) (C57BL/6) MALE 0 MG/KG 25 MG/KG 50 MG/KG 100 200
MG/KG MG/KG
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially In Study 15 15 15 15 15
Early Deaths
Moribund Sacrifice 1
Survivors
Terminal Sacrifice 15 15 15 15 14
Animals Examined Microscopically 15 15 15 15 15
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Liver (15) (15) (15) (15) (15)
Inflammation, Chronic 3 (20%) 6 (40%) 1 (7%) 3 (20%) 1 (7%)
Salivary Glands (1) (1)
Submandibular Gland, Atrophy 1 (100%)
Submandibular Gland, Inflammation, Chronic 1 (100%)
Stomach, Forestomach (15) (15) (15) (15) (15)
Erosion 1 (7%)
Hyperplasia 1 (7%) 3 (20%)
Inflammation, Acute 1 (7%)
Stomach, Glandular (15) (15) (15) (15) (15)
Mineralization 1 (7%)
Glands, Cyst 1 (7%) 1 (7%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (15) (15) (15) (15) (15)
Myocardium, Inflammation, Chronic 1 (7%) 1 (7%)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
None
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
Page 5
NTP Experiment-Test: 99034-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:49:58
____________________________________________________________________________________________________________________________________
P16(INK4A)/(+/-) (C57BL/6) MALE 0 MG/KG 25 MG/KG 50 MG/KG 100 200
MG/KG MG/KG
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Epididymis (15) (15) (15) (15) (15)
Granuloma Sperm 1 (7%)
Unilateral, Hypospermia 1 (7%)
Testes (15) (15) (15) (15) (15)
Germinal Epithelium, Atrophy 1 (7%)
Germinal Epithelium, Syncytial Alteration 1 (7%) 1 (7%)
Rete Testes, Inflammation, Granulomatous 1 (7%)
Unilateral, Germinal Epithelium, Atrophy 1 (7%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (15) (15) (15) (15) (15)
Atrophy 10 (67%) 12 (80%)
Pigmentation, Hemosiderin 1 (7%) 13 (87%) 13 (87%) 15 (100%) 14 (93%)
Lymph Node (11) (11) (12) (9) (7)
Mediastinal, Atrophy 2 (17%) 6 (67%) 6 (86%)
Lymph Node, Mandibular (15) (15) (15) (14) (14)
Atrophy 6 (43%) 11 (79%)
Hyperplasia, Lymphoid 2 (13%) 1 (7%) 1 (7%)
Infiltration Cellular, Polymorphonuclear 1 (7%) 1 (7%)
Lymph Node, Mesenteric (15) (15) (14) (15) (15)
Atrophy 1 (7%) 2 (13%) 2 (14%) 13 (87%) 13 (87%)
Infiltration Cellular, Polymorphonuclear 1 (7%)
Spleen (15) (15) (15) (15) (15)
Hematopoietic Cell Proliferation 1 (7%) 2 (13%) 8 (53%)
Lymphoid Follicle, Atrophy 15 (100%) 14 (93%)
Thymus (15) (15) (15) (15) (15)
Atrophy 7 (47%) 13 (87%)
Mineralization 1 (7%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Skin (15) (15) (15) (15) (15)
Pigmentation 15 (100%) 15 (100%) 15 (100%) 14 (93%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (15) (15) (15) (15) (15)
Pigmentation, Hemosiderin 1 (7%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 6
NTP Experiment-Test: 99034-01 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03
Study Type: SUBCHRON 26-WEEK TRANSGENIC MODEL EVALUATION II (BENZENE) Date: 11/05/03
Route: GAVAGE Time: 14:49:58
____________________________________________________________________________________________________________________________________
P16(INK4A)/(+/-) (C57BL/6) MALE 0 MG/KG 25 MG/KG 50 MG/KG 100 200
MG/KG MG/KG
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
None
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (15) (15) (15) (15) (15)
Inflammation, Chronic Active 2 (13%) 1 (7%) 3 (20%) 5 (33%)
Alveolar Epithelium, Hyperplasia, Focal 1 (7%)
Bronchiole, Hyperplasia, Focal 1 (7%)
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
None
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (15) (15) (15) (15) (15)
Casts Protein 1 (7%) 2 (13%) 1 (7%) 2 (13%)
Nephropathy 1 (7%) 1 (7%) 2 (13%) 4 (27%)
a Number of animals examined microscopically at site and number of animals with lesion
Page 7
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