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TDMS Study 05052-02 Pathology Tables

NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: Battelle Columbus                                     2,4-DICHLOROPHENOL                                  DATE: 09/08/94       
EXPERIMENT: 05052 TEST: 02                                                                                     TIME: 00:04:30       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                   PAGE:       1        
CONT: N01-ES-38068          ROUTE: DOSED FEED                                                                  NTP C#: C55345       
PATHOLOGIST: S. EUSTIS                       G. DILL                                                           CAS: 120-83-2        
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               REASONS FOR REMOVAL:    ALL                                                                                          
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
               REMOVAL DATE RANGE:     ALL                                                                                          
                                                                                                                                    
               TREATMENT GROUPS:       INCLUDE ALL                                                                                  
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08    
LAB: Battelle Columbus                                     2,4-DICHLOROPHENOL                                  DATE: 09/08/94      
EXPERIMENT: 05052 TEST: 02                                                                                     TIME: 00:04:30      
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                       
CONT: N01-ES-38068          ROUTE: DOSED FEED                                                                  NTP C#: C55345      
PATHOLOGIST: S. EUSTIS                       G. DILL                                                           CAS: 120-83-2       
 
 
 
 
 
                                                    MICE(B6C3F1)
                                              ----------------------------
             FOR ALL DOSES THE TUMOR RATES IN THE FOLLOWING TISSUES/ORGANS ARE BASED ON NUMBER OF TISSUES EXAMINED.
             IN OTHER TISSUES/ORGANS, RATES ARE BASED ON NUMBER OF ANIMALS NECROPSIED.
 
 
                                                    ----------------
 
                                                    Adrenal Gland
                                                    Bone Marrow
                                                    Brain
                                                    Epididymis
                                                    Gallbladder
                                                    Heart
                                                    Islets, Pancreatic
                                                    Kidney
                                                    Liver
                                                    Lung
                                                    Nose
                                                    Ovary
                                                    Pancreas
                                                    Parathyroid Gland
                                                    Pituitary Gland
                                                    Preputial Gland
                                                    Prostate
                                                    Salivary Glands
                                                    Spleen
                                                    Testes
                                                    Thymus
                                                    Thyroid Gland
                                                    Urinary Bladder
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: Battelle Columbus                                     2,4-DICHLOROPHENOL                                  DATE: 09/08/94       
EXPERIMENT: 05052 TEST: 02                                                                                     TIME: 00:04:30       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                        
CONT: N01-ES-38068          ROUTE: DOSED FEED                                                                  NTP C#: C55345       
PATHOLOGIST: S. EUSTIS                       G. DILL                                                           CAS: 120-83-2        
 
 
 
 
 
                                     SUMMARY OF STATISTICALLY SIGNIFICANT (P<=.05) RESULTS
                                       IN THE ANALYSIS OF THE STUDY OF 2,4-DICHLOROPHENOL
 
              ====================================================================================================
 
                                                           MALE MICE
                                                          -------------
                                 ORGAN                               MORPHOLOGY
                                -------                             ------------
 
                             Lung                          Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma
                             Skin: Subcutaneous Tissue     Neurofibrosarcoma, Fibrosarcoma, or Sarcoma
                                                           Sarcoma
                             Stomach, Forestomach          Squamous Cell Carcinoma or Papilloma Squamous
                             All Organs                    Malignant Tumors
 
              ====================================================================================================
 
                                                           FEMALE MICE
                                                          -------------
                                 ORGAN                               MORPHOLOGY
                                -------                             ------------
 
                             Liver                         Hepatocellular Adenoma
                                                           Hepatocellular Carcinoma or Hepatocellular Adenoma
                                                           Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma
                             Lung                          Alveolar/Bronchiolar Adenoma
                                                           Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma
                             Stomach, Forestomach          Squamous Cell Carcinoma or Papilloma Squamous
                             All Organs                    Malignant Lymphoma And Histiocytic Sarcoma
                                                           Malignant Lymphoma: Histiocytic, Lymphocytic, Mixed,
                                                            NOS, or Undifferentiated Cell Type
                                                           Malignant Tumors
                                                           Malignant and Benign Tumors
 
              ====================================================================================================
         DATE: 09/08/94                     EXPERIMENT: 05052 TEST: 02                                                 PAGE   1
                             STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 2,4-DICHLOROPHENOL
 
                                                 TERMINAL SACRIFICE AT 104 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      0 PPM           5000 PPM        10000         |      0 PPM           5000 PPM        10000         |
|                       |                                      PPM           |                                      PPM           |
|=================================================================================================================================|
|                                                                                                                                 |
|Adrenal Gland: Non-medullary, Non-cortical                                                                                       |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     1/49 (2%)       0/0 (0%)        1/45 (2%)      |     1/49 (2%)       0/1 (0%)        2/50 (4%)      |
|ADJUSTED (b)           |      3.0%            0.0%            3.2%          |      2.2%            0.0%            4.7%          |
|TERMINAL (d)           |     1/33 (3%)       0/0             1/31 (3%)      |     1/45 (2%)       0/0             2/43 (5%)      |
|FIRST INCIDENCE (DAYS) |      727 (T)         ---             727 (T)       |      728 (T)         ---             728 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.622           (e)           P=0.748        |     P=0.370           (e)           P=0.484        |
|INCIDENTAL TUMOR       |     P=0.622           (e)           P=0.748        |     P=0.370           (e)           P=0.484        |
|LOGISTIC REGRESSION    |     P=0.622           (e)           P=0.748        |     P=0.370           (e)           P=0.484        |
|COCHRAN-ARMITAGE       |     P=0.617                                        |     P=0.391                                        |
|FISHER EXACT           |                     P=1.000N        P=0.731        |                     P=0.980N        P=0.508        |
|=================================================================================================================================|
|                                                                                                                                 |
|Adrenal Medulla                                                                                                                  |
|     Pheochromocytoma Benign                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/49 (0%)       0/0 (0%)        1/45 (2%)      |     2/49 (4%)       0/1 (0%)        2/50 (4%)      |
|ADJUSTED (b)           |      0.0%            0.0%            3.2%          |      4.4%            0.0%            4.7%          |
|TERMINAL (d)           |     0/33 (0%)       0/0             1/31 (3%)      |     2/45 (4%)       0/0             2/43 (5%)      |
|FIRST INCIDENCE (DAYS) |      ---             ---             727 (T)       |      728 (T)         ---             728 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.298           (e)           P=0.488        |     P=0.582           (e)           P=0.678        |
|INCIDENTAL TUMOR       |     P=0.298           (e)           P=0.488        |     P=0.582           (e)           P=0.678        |
|LOGISTIC REGRESSION    |     P=0.298           (e)           P=0.488        |     P=0.582           (e)           P=0.678        |
|COCHRAN-ARMITAGE       |     P=0.293                                        |     P=0.593N                                       |
|FISHER EXACT           |                       (e)           P=0.479        |                     P=0.960N        P=0.684N       |
|=================================================================================================================================|
 
         DATE: 09/08/94                     EXPERIMENT: 05052 TEST: 02                                                 PAGE   2
                             STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 2,4-DICHLOROPHENOL
 
                                                 TERMINAL SACRIFICE AT 104 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      0 PPM           5000 PPM        10000         |      0 PPM           5000 PPM        10000         |
|                       |                                      PPM           |                                      PPM           |
|=================================================================================================================================|
|                                                                                                                                 |
|Harderian Gland                                                                                                                  |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     2/50 (4%)       2/50 (4%)       4/50 (8%)      |     1/50 (2%)       0/50 (0%)       2/50 (4%)      |
|ADJUSTED (b)           |      6.1%            6.3%           12.9%          |      2.2%            0.0%            4.7%          |
|TERMINAL (d)           |     2/33 (6%)       2/32 (6%)       4/31 (13%)     |     1/45 (2%)       0/40 (0%)       2/43 (5%)      |
|FIRST INCIDENCE (DAYS) |      727 (T)         727 (T)         727 (T)       |      728 (T)         ---             728 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.227         P=0.685         P=0.307        |     P=0.351         P=0.523N        P=0.484        |
|INCIDENTAL TUMOR       |     P=0.227         P=0.685         P=0.307        |     P=0.351         P=0.523N        P=0.484        |
|LOGISTIC REGRESSION    |     P=0.227         P=0.685         P=0.307        |     P=0.351         P=0.523N        P=0.484        |
|COCHRAN-ARMITAGE       |     P=0.252                                        |     P=0.360                                        |
|FISHER EXACT           |                     P=0.691N        P=0.339        |                     P=0.500N        P=0.500        |
|=================================================================================================================================|
|                                                                                                                                 |
|Harderian Gland                                                                                                                  |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     3/50 (6%)       2/50 (4%)       4/50 (8%)      |     1/50 (2%)       0/50 (0%)       3/50 (6%)      |
|ADJUSTED (b)           |      9.1%            6.3%           12.9%          |      2.2%            0.0%            6.8%          |
|TERMINAL (d)           |     3/33 (9%)       2/32 (6%)       4/31 (13%)     |     1/45 (2%)       0/40 (0%)       2/43 (5%)      |
|FIRST INCIDENCE (DAYS) |      727 (T)         727 (T)         727 (T)       |      728 (T)         ---             697           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.385         P=0.514N        P=0.465        |     P=0.173         P=0.523N        P=0.293        |
|INCIDENTAL TUMOR       |     P=0.385         P=0.514N        P=0.465        |     P=0.124         P=0.523N        P=0.207        |
|LOGISTIC REGRESSION    |     P=0.385         P=0.514N        P=0.465        |     P=0.166         P=0.523N        P=0.287        |
|COCHRAN-ARMITAGE       |     P=0.417                                        |     P=0.176                                        |
|FISHER EXACT           |                     P=0.500N        P=0.500        |                     P=0.500N        P=0.309        |
|=================================================================================================================================|
 
         DATE: 09/08/94                     EXPERIMENT: 05052 TEST: 02                                                 PAGE   3
                             STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 2,4-DICHLOROPHENOL
 
                                                 TERMINAL SACRIFICE AT 104 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      0 PPM           5000 PPM        10000         |      0 PPM           5000 PPM        10000         |
|                       |                                      PPM           |                                      PPM           |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hemangiosarcoma                                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     1/50 (2%)       2/49 (4%)       0/48 (0%)      |     0/50 (0%)       0/6 (0%)        0/50 (0%)      |
|ADJUSTED (b)           |      2.5%            5.2%            0.0%          |      0.0%            0.0%            0.0%          |
|TERMINAL (d)           |     0/33 (0%)       1/32 (3%)       0/31 (0%)      |     0/45 (0%)       0/5 (0%)        0/43 (0%)      |
|FIRST INCIDENCE (DAYS) |      682             476             ---           |      ---             ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.410N        P=0.476         P=0.551N       |       (e)             (e)             (e)          |
|INCIDENTAL TUMOR       |     P=0.260N        P=0.745         P=0.814N       |       (e)             (e)             (e)          |
|LOGISTIC REGRESSION    |     P=0.351N        P=0.490         P=0.526N       |       (e)             (e)             (e)          |
|COCHRAN-ARMITAGE       |     P=0.371N                                       |       (e)                                          |
|FISHER EXACT           |                     P=0.492         P=0.510N       |                       (e)             (e)          |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Adenoma                                                                                                      |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     4/50 (8%)       5/49 (10%)      6/48 (13%)     |     0/50 (0%)       2/6 (33%)       0/50 (0%)      |
|ADJUSTED (b)           |     12.1%           15.6%           19.4%          |      0.0%           40.0%            0.0%          |
|TERMINAL (d)           |     4/33 (12%)      5/32 (16%)      6/31 (19%)     |     0/45 (0%)       2/5 (40%)       0/43 (0%)      |
|FIRST INCIDENCE (DAYS) |      727 (T)         727 (T)         727 (T)       |      ---             728 (T)         ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.267         P=0.480         P=0.327        |     P=0.631         P<0.001 **        (e)          |
|INCIDENTAL TUMOR       |     P=0.267         P=0.480         P=0.327        |     P=0.631         P<0.001 **        (e)          |
|LOGISTIC REGRESSION    |     P=0.267         P=0.480         P=0.327        |     P=0.631         P<0.001 **        (e)          |
|COCHRAN-ARMITAGE       |     P=0.285                                        |     P=0.643                                        |
|FISHER EXACT           |                     P=0.487         P=0.344        |                     P=0.010 **        (e)          |
|=================================================================================================================================|
 
         DATE: 09/08/94                     EXPERIMENT: 05052 TEST: 02                                                 PAGE   4
                             STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 2,4-DICHLOROPHENOL
 
                                                 TERMINAL SACRIFICE AT 104 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      0 PPM           5000 PPM        10000         |      0 PPM           5000 PPM        10000         |
|                       |                                      PPM           |                                      PPM           |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma                                                                                                    |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     7/50 (14%)      7/49 (14%)      3/48 (6%)      |     2/50 (4%)       0/6 (0%)        1/50 (2%)      |
|ADJUSTED (b)           |     18.6%           18.7%            9.4%          |      4.4%            0.0%            2.3%          |
|TERMINAL (d)           |     4/33 (12%)      3/32 (9%)       2/31 (6%)      |     2/45 (4%)       0/5 (0%)        1/43 (2%)      |
|FIRST INCIDENCE (DAYS) |      605             665             675           |      728 (T)         ---             728 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.203N        P=0.567         P=0.222N       |     P=0.397N        P=0.763N        P=0.516N       |
|INCIDENTAL TUMOR       |     P=0.457N        P=0.517         P=0.445N       |     P=0.397N        P=0.763N        P=0.516N       |
|LOGISTIC REGRESSION    |     P=0.401N        P=0.567         P=0.242N       |     P=0.397N        P=0.763N        P=0.516N       |
|COCHRAN-ARMITAGE       |     P=0.151N                                       |     P=0.382N                                       |
|FISHER EXACT           |                     P=0.597         P=0.176N       |                     P=0.795N        P=0.500N       |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma or Hepatocellular Adenoma                                                                          |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    10/50 (20%)     12/49 (24%)      9/48 (19%)     |     2/50 (4%)       2/6 (33%)       1/50 (2%)      |
|ADJUSTED (b)           |     27.0%           32.7%           28.1%          |      4.4%           40.0%            2.3%          |
|TERMINAL (d)           |     7/33 (21%)      8/32 (25%)      8/31 (26%)     |     2/45 (4%)       2/5 (40%)       1/43 (2%)      |
|FIRST INCIDENCE (DAYS) |      605             665             675           |      728 (T)         728 (T)         728 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.541         P=0.364         P=0.592N       |     P=0.427N        P=0.029 *       P=0.516N       |
|INCIDENTAL TUMOR       |     P=0.302         P=0.315         P=0.405        |     P=0.427N        P=0.029 *       P=0.516N       |
|LOGISTIC REGRESSION    |     P=0.430         P=0.352         P=0.494        |     P=0.427N        P=0.029 *       P=0.516N       |
|COCHRAN-ARMITAGE       |     P=0.492N                                       |     P=0.407N                                       |
|FISHER EXACT           |                     P=0.384         P=0.540N       |                     P=0.053         P=0.500N       |
|=================================================================================================================================|
 
         DATE: 09/08/94                     EXPERIMENT: 05052 TEST: 02                                                 PAGE   5
                             STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 2,4-DICHLOROPHENOL
 
                                                 TERMINAL SACRIFICE AT 104 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      0 PPM           5000 PPM        10000         |      0 PPM           5000 PPM        10000         |
|                       |                                      PPM           |                                      PPM           |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma                                                         |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    10/50 (20%)     12/49 (24%)      9/48 (19%)     |     2/50 (4%)       2/6 (33%)       1/50 (2%)      |
|ADJUSTED (b)           |     27.0%           32.7%           28.1%          |      4.4%           40.0%            2.3%          |
|TERMINAL (d)           |     7/33 (21%)      8/32 (25%)      8/31 (26%)     |     2/45 (4%)       2/5 (40%)       1/43 (2%)      |
|FIRST INCIDENCE (DAYS) |      605             665             675           |      728 (T)         728 (T)         728 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.541         P=0.364         P=0.592N       |     P=0.427N        P=0.029 *       P=0.516N       |
|INCIDENTAL TUMOR       |     P=0.302         P=0.315         P=0.405        |     P=0.427N        P=0.029 *       P=0.516N       |
|LOGISTIC REGRESSION    |     P=0.430         P=0.352         P=0.494        |     P=0.427N        P=0.029 *       P=0.516N       |
|COCHRAN-ARMITAGE       |     P=0.492N                                       |     P=0.407N                                       |
|FISHER EXACT           |                     P=0.384         P=0.540N       |                     P=0.053         P=0.500N       |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Adenoma                                                                                                |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     2/50 (4%)       1/4 (25%)       3/50 (6%)      |     2/50 (4%)       1/1 (100%)      2/50 (4%)      |
|ADJUSTED (b)           |      6.1%          100.0%            9.7%          |      4.4%          100.0%            4.7%          |
|TERMINAL (d)           |     2/33 (6%)       1/1 (100%)      3/31 (10%)     |     2/45 (4%)       1/1 (100%)      2/43 (5%)      |
|FIRST INCIDENCE (DAYS) |      727 (T)         727 (T)         727 (T)       |      728 (T)         728 (T)         728 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.385         P=0.073         P=0.471        |     P=0.571         P=0.039 *       P=0.678        |
|INCIDENTAL TUMOR       |     P=0.385         P=0.073         P=0.471        |     P=0.571         P=0.039 *       P=0.678        |
|LOGISTIC REGRESSION    |     P=0.385         P=0.073         P=0.471        |     P=0.571         P=0.039 *       P=0.678        |
|COCHRAN-ARMITAGE       |     P=0.415                                        |     P=0.591                                        |
|FISHER EXACT           |                     P=0.210         P=0.500        |                     P=0.059         P=0.691N       |
|=================================================================================================================================|
 
         DATE: 09/08/94                     EXPERIMENT: 05052 TEST: 02                                                 PAGE   6
                             STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 2,4-DICHLOROPHENOL
 
                                                 TERMINAL SACRIFICE AT 104 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      0 PPM           5000 PPM        10000         |      0 PPM           5000 PPM        10000         |
|                       |                                      PPM           |                                      PPM           |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Carcinoma                                                                                              |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     1/50 (2%)       1/4 (25%)       2/50 (4%)      |     1/50 (2%)       0/1 (0%)        0/50 (0%)      |
|ADJUSTED (b)           |      3.0%            2.8%            6.5%          |      2.1%            0.0%            0.0%          |
|TERMINAL (d)           |     1/33 (3%)       0/1 (0%)        2/31 (6%)      |     0/45 (0%)       0/1 (0%)        0/43 (0%)      |
|FIRST INCIDENCE (DAYS) |      727 (T)         681             727 (T)       |      668             ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.359         P=0.502         P=0.478        |     P=0.285N        P=0.527N        P=0.517N       |
|INCIDENTAL TUMOR       |     P=0.286         P=0.229         P=0.478        |     P=0.500N          (e)           P=0.718N       |
|LOGISTIC REGRESSION    |     P=0.324         P=0.148         P=0.478        |     P=0.267N          (e)           P=0.465N       |
|COCHRAN-ARMITAGE       |     P=0.397                                        |     P=0.307N                                       |
|FISHER EXACT           |                     P=0.144         P=0.500        |                     P=0.980N        P=0.500N       |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma                                                              |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     3/50 (6%)       2/4 (50%)       5/50 (10%)     |     3/50 (6%)       1/1 (100%)      2/50 (4%)      |
|ADJUSTED (b)           |      9.1%          100.0%           16.1%          |      6.4%          100.0%            4.7%          |
|TERMINAL (d)           |     3/33 (9%)       1/1 (100%)      5/31 (16%)     |     2/45 (4%)       1/1 (100%)      2/43 (5%)      |
|FIRST INCIDENCE (DAYS) |      727 (T)         681             727 (T)       |      668             728 (T)         728 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.260         P=0.063         P=0.320        |     P=0.439N        P=0.523         P=0.524N       |
|INCIDENTAL TUMOR       |     P=0.219         P=0.007 **      P=0.320        |     P=0.519N        P=0.039 *       P=0.608N       |
|LOGISTIC REGRESSION    |     P=0.207         P=0.009 **      P=0.320        |     P=0.437N        P=0.051         P=0.516N       |
|COCHRAN-ARMITAGE       |     P=0.305                                        |     P=0.416N                                       |
|FISHER EXACT           |                     P=0.039 *       P=0.357        |                     P=0.078         P=0.500N       |
|=================================================================================================================================|
 
         DATE: 09/08/94                     EXPERIMENT: 05052 TEST: 02                                                 PAGE   7
                             STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 2,4-DICHLOROPHENOL
 
                                                 TERMINAL SACRIFICE AT 104 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      0 PPM           5000 PPM        10000         |      0 PPM           5000 PPM        10000         |
|                       |                                      PPM           |                                      PPM           |
|=================================================================================================================================|
|                                                                                                                                 |
|Pituitary Gland: Pars Distalis or Unspecified Site                                                                               |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/45 (0%)       0/0 (0%)        0/47 (0%)      |     4/49 (8%)       0/0 (0%)        3/48 (6%)      |
|ADJUSTED (b)           |      0.0%            0.0%            0.0%          |      8.9%            0.0%            7.0%          |
|TERMINAL (d)           |     0/29 (0%)       0/0             0/30 (0%)      |     4/45 (9%)       0/0             3/43 (7%)      |
|FIRST INCIDENCE (DAYS) |      ---             ---             ---           |      728 (T)         ---             728 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |       (e)             (e)             (e)          |     P=0.447N          (e)           P=0.525N       |
|INCIDENTAL TUMOR       |       (e)             (e)             (e)          |     P=0.447N          (e)           P=0.525N       |
|LOGISTIC REGRESSION    |       (e)             (e)             (e)          |     P=0.447N          (e)           P=0.525N       |
|COCHRAN-ARMITAGE       |       (e)                                          |     P=0.433N                                       |
|FISHER EXACT           |                       (e)             (e)          |                     P=1.000N        P=0.512N       |
|=================================================================================================================================|
|                                                                                                                                 |
|Pituitary Gland: Pars Distalis or Unspecified Site                                                                               |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/45 (0%)       0/0 (0%)        0/47 (0%)      |     4/49 (8%)       0/0 (0%)        4/48 (8%)      |
|ADJUSTED (b)           |      0.0%            0.0%            0.0%          |      8.9%            0.0%            9.3%          |
|TERMINAL (d)           |     0/29 (0%)       0/0             0/30 (0%)      |     4/45 (9%)       0/0             4/43 (9%)      |
|FIRST INCIDENCE (DAYS) |      ---             ---             ---           |      728 (T)         ---             728 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |       (e)             (e)             (e)          |     P=0.547           (e)           P=0.619        |
|INCIDENTAL TUMOR       |       (e)             (e)             (e)          |     P=0.547           (e)           P=0.619        |
|LOGISTIC REGRESSION    |       (e)             (e)             (e)          |     P=0.547           (e)           P=0.619        |
|COCHRAN-ARMITAGE       |       (e)                                          |     P=0.561                                        |
|FISHER EXACT           |                       (e)             (e)          |                     P=1.000N        P=0.631        |
|=================================================================================================================================|
 
         DATE: 09/08/94                     EXPERIMENT: 05052 TEST: 02                                                 PAGE   8
                             STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 2,4-DICHLOROPHENOL
 
                                                 TERMINAL SACRIFICE AT 104 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      0 PPM           5000 PPM        10000         |      0 PPM           5000 PPM        10000         |
|                       |                                      PPM           |                                      PPM           |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin: Subcutaneous Tissue                                                                                                        |
|     Fibroma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     2/50 (4%)       5/50 (10%)      3/50 (6%)      |     0/50 (0%)       0/50 (0%)       0/50 (0%)      |
|ADJUSTED (b)           |      6.1%           15.6%            9.7%          |      0.0%            0.0%            0.0%          |
|TERMINAL (d)           |     2/33 (6%)       5/32 (16%)      3/31 (10%)     |     0/45 (0%)       0/40 (0%)       0/43 (0%)      |
|FIRST INCIDENCE (DAYS) |      727 (T)         727 (T)         727 (T)       |      ---             ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.387         P=0.201         P=0.471        |       (e)             (e)             (e)          |
|INCIDENTAL TUMOR       |     P=0.387         P=0.201         P=0.471        |       (e)             (e)             (e)          |
|LOGISTIC REGRESSION    |     P=0.387         P=0.201         P=0.471        |       (e)             (e)             (e)          |
|COCHRAN-ARMITAGE       |     P=0.421                                        |       (e)                                          |
|FISHER EXACT           |                     P=0.218         P=0.500        |                       (e)             (e)          |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin: Subcutaneous Tissue                                                                                                        |
|     Fibrosarcoma                                                                                                                |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     1/50 (2%)       5/50 (10%)      1/50 (2%)      |     0/50 (0%)       0/50 (0%)       0/50 (0%)      |
|ADJUSTED (b)           |      2.6%           13.3%            3.2%          |      0.0%            0.0%            0.0%          |
|TERMINAL (d)           |     0/33 (0%)       2/32 (6%)       1/31 (3%)      |     0/45 (0%)       0/40 (0%)       0/43 (0%)      |
|FIRST INCIDENCE (DAYS) |      687             510             727 (T)       |      ---             ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.535         P=0.099         P=0.731        |       (e)             (e)             (e)          |
|INCIDENTAL TUMOR       |     P=0.532         P=0.154         P=0.629        |       (e)             (e)             (e)          |
|LOGISTIC REGRESSION    |     P=0.562         P=0.103         P=0.724        |       (e)             (e)             (e)          |
|COCHRAN-ARMITAGE       |     P=0.594                                        |       (e)                                          |
|FISHER EXACT           |                     P=0.102         P=0.753N       |                       (e)             (e)          |
|=================================================================================================================================|
 
         DATE: 09/08/94                     EXPERIMENT: 05052 TEST: 02                                                 PAGE   9
                             STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 2,4-DICHLOROPHENOL
 
                                                 TERMINAL SACRIFICE AT 104 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      0 PPM           5000 PPM        10000         |      0 PPM           5000 PPM        10000         |
|                       |                                      PPM           |                                      PPM           |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin: Subcutaneous Tissue                                                                                                        |
|     Neurofibrosarcoma, Fibrosarcoma, Sarcoma,                                                                                   |
|     Neurofibroma, or Fibroma                                                                                                    |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    10/50 (20%)     14/50 (28%)      5/50 (10%)     |     0/50 (0%)       0/50 (0%)       1/50 (2%)      |
|ADJUSTED (b)           |     24.1%           36.1%           16.1%          |      0.0%            0.0%            2.1%          |
|TERMINAL (d)           |     3/33 (9%)       8/32 (25%)      5/31 (16%)     |     0/45 (0%)       0/40 (0%)       0/43 (0%)      |
|FIRST INCIDENCE (DAYS) |      651             510             727 (T)       |      ---             ---             549           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.215N        P=0.220         P=0.211N       |     P=0.263           (e)           P=0.488        |
|INCIDENTAL TUMOR       |     P=0.389N        P=0.206         P=0.502N       |     P=0.315           (e)           P=0.581        |
|LOGISTIC REGRESSION    |     P=0.209N        P=0.221         P=0.219N       |     P=0.350           (e)           P=0.669        |
|COCHRAN-ARMITAGE       |     P=0.127N                                       |     P=0.269                                        |
|FISHER EXACT           |                     P=0.241         P=0.131N       |                       (e)           P=0.500        |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin: Subcutaneous Tissue                                                                                                        |
|     Neurofibrosarcoma, Fibrosarcoma, or Sarcoma                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     8/50 (16%)     10/50 (20%)      2/50 (4%)      |     0/50 (0%)       0/50 (0%)       1/50 (2%)      |
|ADJUSTED (b)           |     19.1%           25.5%            6.5%          |      0.0%            0.0%            2.1%          |
|TERMINAL (d)           |     1/33 (3%)       4/32 (13%)      2/31 (6%)      |     0/45 (0%)       0/40 (0%)       0/43 (0%)      |
|FIRST INCIDENCE (DAYS) |      651             510             727 (T)       |      ---             ---             549           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.106N        P=0.356         P=0.092N       |     P=0.263           (e)           P=0.488        |
|INCIDENTAL TUMOR       |     P=0.221N        P=0.368         P=0.312N       |     P=0.315           (e)           P=0.581        |
|LOGISTIC REGRESSION    |     P=0.172N        P=0.261         P=0.071N       |     P=0.350           (e)           P=0.669        |
|COCHRAN-ARMITAGE       |     P=0.053N                                       |     P=0.269                                        |
|FISHER EXACT           |                     P=0.398         P=0.046N*      |                       (e)           P=0.500        |
|=================================================================================================================================|
 
         DATE: 09/08/94                     EXPERIMENT: 05052 TEST: 02                                                 PAGE  10
                             STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 2,4-DICHLOROPHENOL
 
                                                 TERMINAL SACRIFICE AT 104 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      0 PPM           5000 PPM        10000         |      0 PPM           5000 PPM        10000         |
|                       |                                      PPM           |                                      PPM           |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin: Subcutaneous Tissue                                                                                                        |
|     Sarcoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     7/50 (14%)      5/50 (10%)      1/50 (2%)      |     0/50 (0%)       0/50 (0%)       1/50 (2%)      |
|ADJUSTED (b)           |     16.9%           13.7%            3.2%          |      0.0%            0.0%            2.1%          |
|TERMINAL (d)           |     1/33 (3%)       2/32 (6%)       1/31 (3%)      |     0/45 (0%)       0/40 (0%)       0/43 (0%)      |
|FIRST INCIDENCE (DAYS) |      651             547             727 (T)       |      ---             ---             549           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.050N*       P=0.432N        P=0.063N       |     P=0.263           (e)           P=0.488        |
|INCIDENTAL TUMOR       |     P=0.147N        P=0.486N        P=0.209N       |     P=0.315           (e)           P=0.581        |
|LOGISTIC REGRESSION    |     P=0.034N*       P=0.483N        P=0.115N       |     P=0.350           (e)           P=0.669        |
|COCHRAN-ARMITAGE       |     P=0.025N*                                      |     P=0.269                                        |
|FISHER EXACT           |                     P=0.380N        P=0.030N*      |                       (e)           P=0.500        |
|=================================================================================================================================|
|                                                                                                                                 |
|Stomach, Forestomach                                                                                                             |
|     Squamous Cell Carcinoma or Papilloma Squamous                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/50 (0%)       0/50 (0%)       3/50 (6%)      |     4/50 (8%)       3/50 (6%)       0/50 (0%)      |
|ADJUSTED (b)           |      0.0%            0.0%            9.7%          |      8.9%            7.5%            0.0%          |
|TERMINAL (d)           |     0/33 (0%)       0/32 (0%)       3/31 (10%)     |     4/45 (9%)       3/40 (8%)       0/43 (0%)      |
|FIRST INCIDENCE (DAYS) |      ---             ---             727 (T)       |      728 (T)         728 (T)         ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.033 *         (e)           P=0.110        |     P=0.057N        P=0.564N        P=0.069N       |
|INCIDENTAL TUMOR       |     P=0.033 *         (e)           P=0.110        |     P=0.057N        P=0.564N        P=0.069N       |
|LOGISTIC REGRESSION    |     P=0.033 *         (e)           P=0.110        |     P=0.057N        P=0.564N        P=0.069N       |
|COCHRAN-ARMITAGE       |     P=0.037 *                                      |     P=0.049N*                                      |
|FISHER EXACT           |                       (e)           P=0.121        |                     P=0.500N        P=0.059N       |
|=================================================================================================================================|
 
         DATE: 09/08/94                     EXPERIMENT: 05052 TEST: 02                                                 PAGE  11
                             STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 2,4-DICHLOROPHENOL
 
                                                 TERMINAL SACRIFICE AT 104 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      0 PPM           5000 PPM        10000         |      0 PPM           5000 PPM        10000         |
|                       |                                      PPM           |                                      PPM           |
|=================================================================================================================================|
|                                                                                                                                 |
|Stomach, Forestomach                                                                                                             |
|     Squamous Cell Papilloma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     0/50 (0%)       0/50 (0%)       2/50 (4%)      |     3/50 (6%)       3/50 (6%)       0/50 (0%)      |
|ADJUSTED (b)           |      0.0%            0.0%            6.5%          |      6.7%            7.5%            0.0%          |
|TERMINAL (d)           |     0/33 (0%)       0/32 (0%)       2/31 (6%)      |     3/45 (7%)       3/40 (8%)       0/43 (0%)      |
|FIRST INCIDENCE (DAYS) |      ---             ---             727 (T)       |      728 (T)         728 (T)         ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.090           (e)           P=0.224        |     P=0.113N        P=0.608         P=0.130N       |
|INCIDENTAL TUMOR       |     P=0.090           (e)           P=0.224        |     P=0.113N        P=0.608         P=0.130N       |
|LOGISTIC REGRESSION    |     P=0.090           (e)           P=0.224        |     P=0.113N        P=0.608         P=0.130N       |
|COCHRAN-ARMITAGE       |     P=0.095                                        |     P=0.101N                                       |
|FISHER EXACT           |                       (e)           P=0.247        |                     P=0.661N        P=0.121N       |
|=================================================================================================================================|
|                                                                                                                                 |
|Thyroid Gland: Follicular Cell                                                                                                   |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     2/50 (4%)       0/0 (0%)        0/48 (0%)      |     1/49 (2%)       0/0 (0%)        0/47 (0%)      |
|ADJUSTED (b)           |      6.1%            0.0%            0.0%          |      2.2%            0.0%            0.0%          |
|TERMINAL (d)           |     2/33 (6%)       0/0             0/31 (0%)      |     1/45 (2%)       0/0             0/43 (0%)      |
|FIRST INCIDENCE (DAYS) |      727 (T)         ---             ---           |      728 (T)         ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.153N          (e)           P=0.252N       |     P=0.317N          (e)           P=0.509N       |
|INCIDENTAL TUMOR       |     P=0.153N          (e)           P=0.252N       |     P=0.317N          (e)           P=0.509N       |
|LOGISTIC REGRESSION    |     P=0.153N          (e)           P=0.252N       |     P=0.317N          (e)           P=0.509N       |
|COCHRAN-ARMITAGE       |     P=0.149N                                       |     P=0.315N                                       |
|FISHER EXACT           |                     P=1.000N        P=0.258N       |                     P=1.000N        P=0.510N       |
|=================================================================================================================================|
 
         DATE: 09/08/94                     EXPERIMENT: 05052 TEST: 02                                                 PAGE  12
                             STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 2,4-DICHLOROPHENOL
 
                                                 TERMINAL SACRIFICE AT 104 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      0 PPM           5000 PPM        10000         |      0 PPM           5000 PPM        10000         |
|                       |                                      PPM           |                                      PPM           |
|=================================================================================================================================|
|                                                                                                                                 |
|Uterus                                                                                                                           |
|     Polyp Stromal                                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |     2/50 (4%)       2/50 (4%)       1/50 (2%)      |
|ADJUSTED (b)           |                                                    |      4.4%            5.0%            2.3%          |
|TERMINAL (d)           |                                                    |     2/45 (4%)       2/40 (5%)       1/43 (2%)      |
|FIRST INCIDENCE (DAYS) |                                                    |      728 (T)         728 (T)         728 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |     P=0.409N        P=0.652         P=0.516N       |
|INCIDENTAL TUMOR       |                                                    |     P=0.409N        P=0.652         P=0.516N       |
|LOGISTIC REGRESSION    |                                                    |     P=0.409N        P=0.652         P=0.516N       |
|COCHRAN-ARMITAGE       |                                                    |     P=0.390N                                       |
|FISHER EXACT           |                                                    |                     P=0.691N        P=0.500N       |
|=================================================================================================================================|
|                                                                                                                                 |
|Uterus                                                                                                                           |
|     Sarcoma Stromal or Polyp Stromal                                                                                            |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |     2/50 (4%)       2/50 (4%)       2/50 (4%)      |
|ADJUSTED (b)           |                                                    |      4.4%            5.0%            4.7%          |
|TERMINAL (d)           |                                                    |     2/45 (4%)       2/40 (5%)       2/43 (5%)      |
|FIRST INCIDENCE (DAYS) |                                                    |      728 (T)         728 (T)         728 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |     P=0.581         P=0.652         P=0.678        |
|INCIDENTAL TUMOR       |                                                    |     P=0.581         P=0.652         P=0.678        |
|LOGISTIC REGRESSION    |                                                    |     P=0.581         P=0.652         P=0.678        |
|COCHRAN-ARMITAGE       |                                                    |     P=0.601                                        |
|FISHER EXACT           |                                                    |                     P=0.691N        P=0.691N       |
|=================================================================================================================================|
 
         DATE: 09/08/94                     EXPERIMENT: 05052 TEST: 02                                                 PAGE  13
                             STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 2,4-DICHLOROPHENOL
 
                                                 TERMINAL SACRIFICE AT 104 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      0 PPM           5000 PPM        10000         |      0 PPM           5000 PPM        10000         |
|                       |                                      PPM           |                                      PPM           |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangiosarcoma                                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     1/50 (2%)       2/50 (4%)       0/50 (0%)      |     1/50 (2%)       0/50 (0%)       0/50 (0%)      |
|ADJUSTED (b)           |      2.5%            5.2%            0.0%          |      2.2%            0.0%            0.0%          |
|TERMINAL (d)           |     0/33 (0%)       1/32 (3%)       0/31 (0%)      |     1/45 (2%)       0/40 (0%)       0/43 (0%)      |
|FIRST INCIDENCE (DAYS) |      682             476             ---           |      728 (T)         ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.410N        P=0.476         P=0.551N       |     P=0.280N        P=0.523N        P=0.509N       |
|INCIDENTAL TUMOR       |     P=0.240N        P=0.745         P=0.739N       |     P=0.280N        P=0.523N        P=0.509N       |
|LOGISTIC REGRESSION    |     P=0.343N        P=0.509         P=0.519N       |     P=0.280N        P=0.523N        P=0.509N       |
|COCHRAN-ARMITAGE       |     P=0.360N                                       |     P=0.269N                                       |
|FISHER EXACT           |                     P=0.500         P=0.500N       |                     P=0.500N        P=0.500N       |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangiosarcoma or Hemangioma                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     1/50 (2%)       2/50 (4%)       0/50 (0%)      |     1/50 (2%)       0/50 (0%)       0/50 (0%)      |
|ADJUSTED (b)           |      2.5%            5.2%            0.0%          |      2.2%            0.0%            0.0%          |
|TERMINAL (d)           |     0/33 (0%)       1/32 (3%)       0/31 (0%)      |     1/45 (2%)       0/40 (0%)       0/43 (0%)      |
|FIRST INCIDENCE (DAYS) |      682             476             ---           |      728 (T)         ---             ---           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.410N        P=0.476         P=0.551N       |     P=0.280N        P=0.523N        P=0.509N       |
|INCIDENTAL TUMOR       |     P=0.240N        P=0.745         P=0.739N       |     P=0.280N        P=0.523N        P=0.509N       |
|LOGISTIC REGRESSION    |     P=0.343N        P=0.509         P=0.519N       |     P=0.280N        P=0.523N        P=0.509N       |
|COCHRAN-ARMITAGE       |     P=0.360N                                       |     P=0.269N                                       |
|FISHER EXACT           |                     P=0.500         P=0.500N       |                     P=0.500N        P=0.500N       |
|=================================================================================================================================|
 
         DATE: 09/08/94                     EXPERIMENT: 05052 TEST: 02                                                 PAGE  14
                             STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 2,4-DICHLOROPHENOL
 
                                                 TERMINAL SACRIFICE AT 104 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      0 PPM           5000 PPM        10000         |      0 PPM           5000 PPM        10000         |
|                       |                                      PPM           |                                      PPM           |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Lymphoma And Histiocytic Sarcoma                                                                                  |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     3/50 (6%)       3/50 (6%)       4/50 (8%)      |    12/50 (24%)      6/50 (12%)      4/50 (8%)      |
|ADJUSTED (b)           |      7.2%            8.2%           11.1%          |     26.1%           14.1%            9.3%          |
|TERMINAL (d)           |     1/33 (3%)       2/32 (6%)       2/31 (6%)      |    11/45 (24%)      4/40 (10%)      4/43 (9%)      |
|FIRST INCIDENCE (DAYS) |      575             469             394           |      679             616             728 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.354         P=0.637         P=0.423        |     P=0.025N*       P=0.156N        P=0.036N*      |
|INCIDENTAL TUMOR       |     P=0.452         P=0.669N        P=0.429        |     P=0.029N*       P=0.133N        P=0.046N*      |
|LOGISTIC REGRESSION    |     P=0.456         P=0.653N        P=0.539        |     P=0.022N*       P=0.118N        P=0.036N*      |
|COCHRAN-ARMITAGE       |     P=0.421                                        |     P=0.017N*                                      |
|FISHER EXACT           |                     P=0.661N        P=0.500        |                     P=0.096N        P=0.027N*      |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Lymphoma: Histiocytic, Lymphocytic, Mixed,                                                                        |
|     NOS, or Undifferentiated Cell Type                                                                                          |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |     3/50 (6%)       3/50 (6%)       4/50 (8%)      |    12/50 (24%)      6/50 (12%)      4/50 (8%)      |
|ADJUSTED (b)           |      7.2%            8.2%           11.1%          |     26.1%           14.1%            9.3%          |
|TERMINAL (d)           |     1/33 (3%)       2/32 (6%)       2/31 (6%)      |    11/45 (24%)      4/40 (10%)      4/43 (9%)      |
|FIRST INCIDENCE (DAYS) |      575             469             394           |      679             616             728 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.354         P=0.637         P=0.423        |     P=0.025N*       P=0.156N        P=0.036N*      |
|INCIDENTAL TUMOR       |     P=0.452         P=0.669N        P=0.429        |     P=0.029N*       P=0.133N        P=0.046N*      |
|LOGISTIC REGRESSION    |     P=0.456         P=0.653N        P=0.539        |     P=0.022N*       P=0.118N        P=0.036N*      |
|COCHRAN-ARMITAGE       |     P=0.421                                        |     P=0.017N*                                      |
|FISHER EXACT           |                     P=0.661N        P=0.500        |                     P=0.096N        P=0.027N*      |
|=================================================================================================================================|
 
         DATE: 09/08/94                     EXPERIMENT: 05052 TEST: 02                                                 PAGE  15
                             STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 2,4-DICHLOROPHENOL
 
                                                 TERMINAL SACRIFICE AT 104 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      0 PPM           5000 PPM        10000         |      0 PPM           5000 PPM        10000         |
|                       |                                      PPM           |                                      PPM           |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Benign Tumors                                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    11/50 (22%)     13/50 (26%)     16/50 (32%)     |    16/50 (32%)      8/50 (16%)     10/50 (20%)     |
|ADJUSTED (b)           |     33.3%           40.6%           51.6%          |     35.6%           20.0%           23.3%          |
|TERMINAL (d)           |    11/33 (33%)     13/32 (41%)     16/31 (52%)     |    16/45 (36%)      8/40 (20%)     10/43 (23%)     |
|FIRST INCIDENCE (DAYS) |      727 (T)         727 (T)         727 (T)       |      728 (T)         728 (T)         728 (T)       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.089         P=0.363         P=0.112        |     P=0.116N        P=0.090N        P=0.153N       |
|INCIDENTAL TUMOR       |     P=0.089         P=0.363         P=0.112        |     P=0.116N        P=0.090N        P=0.153N       |
|LOGISTIC REGRESSION    |     P=0.089         P=0.363         P=0.112        |     P=0.116N        P=0.090N        P=0.153N       |
|COCHRAN-ARMITAGE       |     P=0.154                                        |     P=0.094N                                       |
|FISHER EXACT           |                     P=0.408         P=0.184        |                     P=0.050N        P=0.127N       |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Tumors                                                                                                            |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    21/50 (42%)     21/50 (42%)     10/50 (20%)     |    16/50 (32%)      6/50 (12%)     12/50 (24%)     |
|ADJUSTED (b)           |     45.1%           47.4%           28.7%          |     34.0%           14.1%           25.0%          |
|TERMINAL (d)           |     8/33 (24%)      9/32 (28%)      7/31 (23%)     |    14/45 (31%)      4/40 (10%)      7/43 (16%)     |
|FIRST INCIDENCE (DAYS) |      561             469             394           |      668             616             445           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.073N        P=0.487         P=0.069N       |     P=0.255N        P=0.035N*       P=0.314N       |
|INCIDENTAL TUMOR       |     P=0.108N        P=0.570         P=0.217N       |     P=0.252N        P=0.024N*       P=0.270N       |
|LOGISTIC REGRESSION    |     P=0.069N        P=0.515         P=0.077N       |     P=0.187N        P=0.020N*       P=0.215N       |
|COCHRAN-ARMITAGE       |     P=0.014N*                                      |     P=0.202N                                       |
|FISHER EXACT           |                     P=0.580N        P=0.015N*      |                     P=0.014N*       P=0.252N       |
|=================================================================================================================================|
 
         DATE: 09/08/94                     EXPERIMENT: 05052 TEST: 02                                                 PAGE  16
                             STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- 2,4-DICHLOROPHENOL
 
                                                 TERMINAL SACRIFICE AT 104 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |      0 PPM           5000 PPM        10000         |      0 PPM           5000 PPM        10000         |
|                       |                                      PPM           |                                      PPM           |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant and Benign Tumors                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |       #               #               #            |       #               #               #            |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |    29/50 (58%)     29/50 (58%)     24/50 (48%)     |    26/50 (52%)     14/50 (28%)     22/50 (44%)     |
|ADJUSTED (b)           |     62.7%           65.7%           70.3%          |     55.3%           33.2%           45.8%          |
|TERMINAL (d)           |    16/33 (48%)     17/32 (53%)     21/31 (68%)     |    24/45 (53%)     12/40 (30%)     17/43 (40%)     |
|FIRST INCIDENCE (DAYS) |      561             469             394           |      668             616             445           |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |     P=0.407N        P=0.472         P=0.423N       |     P=0.316N        P=0.041N*       P=0.364N       |
|INCIDENTAL TUMOR       |     P=0.439         P=0.541         P=0.350        |     P=0.321N        P=0.031N*       P=0.330N       |
|LOGISTIC REGRESSION    |     P=0.477         P=0.487         P=0.517N       |     P=0.283N        P=0.025N*       P=0.298N       |
|COCHRAN-ARMITAGE       |     P=0.183N                                       |     P=0.239N                                       |
|FISHER EXACT           |                     P=0.580N        P=0.212N       |                     P=0.012N*       P=0.274N       |
|=================================================================================================================================|
 
 (a)  Number of tumor-bearing animals / number of animals examined at site.
 (b)  Kaplan-Meier estimated lifetime tumor incidence after adjustment for 
      intercurrent mortality.
 (d)  Observed incidence at terminal kill.
 (f)  Beneath the control incidence are the P-values associated with the trend 
      test.  Beneath the dosed group incidence are the P-values corresponding to 
      pairwise comparisons between the controls and that dosed group.  The life
      table analysis regards tumors in animals dying  prior to terminal kill as
      being (directly or indirectly) the cause of death.  The incidental tumor 
      test regards these lesions as non-fatal.  Logistic regression is an alternative
      method for analyzing the incidence of non-fatal tumors.  The Cochran-Armitage
      and Fisher's exact tests compare directly the overall incidence rates.  
      For all tests, a negative trend is indicated by "N".
 (e)  Value of Statistic cannot be computed. 
 (I)  Interim sacrifice
 (T)  Terminal sacrifice
  #   Tumor rates based on number of animals necropsied.
 
  *   To the right of any statistical result, indicates significance at (P<=0.05).
  **  To the right of any statistical result, indicates significance at (P<=0.01).

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