National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/3895

TDMS Study 05069-06 Pathology Tables

NTP                                         STATISTICAL ANALYSIS OF SURVIVAL DATA                              REPORT: PEIRPT11    
LAB: TSI Mason Research                      1,2-DIHYDRO-2,2,4-TRIMETHYLQUINOLINE_(MONOMER)                    DATE: 04/05/96      
EXPERIMENT: 05069 TEST: 06                                                                                     TIME: 06:12:38      
TEST TYPE:  INIT/PROMOT                         CAGES FROM 0000 TO LAST CAGE                                                       
CONT: N01-ES-95256                              ROUTE: DERMAL,SOLUTION                                         NTP C#: 60902B      
PATHOLOGIST: M.E.P. GOAD                                                                                       CAS: 147-47-7       
                  1,2-DIHYDRO-2,2,4-TRIMETHYLQUINOLINE_(MONOMER)

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                    FEMALE MICE
                       FIRST TERMINAL SACRIFICE AT 372 DAYS
                                                          

                          INDIVIDUAL SURVIVAL TIMES (DAYS)
 ==================================================================================
 |                          DOSE = DMBA/TMQ5 MG/KG                                |
 |                       ----------------------------                             |
 |                                                                                |
 |  TOTAL 30     UNCENSORED DEATHS 2      CENSORED DEATHS 0    TERMINAL 28        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      346   355                                                                 |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                          DOSE = DMBA/TMQ10 MG/KG                               |
 |                       ----------------------------                             |
 |                                                                                |
 |  TOTAL 30     UNCENSORED DEATHS 2      CENSORED DEATHS 0    TERMINAL 28        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      240   313                                                                 |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                          DOSE = DMBA/TMQ25 MG/KG                               |
 |                       ----------------------------                             |
 |                                                                                |
 |  TOTAL 30     UNCENSORED DEATHS 3      CENSORED DEATHS 0    TERMINAL 27        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      52    242   365                                                           |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 ==================================================================================
                  1,2-DIHYDRO-2,2,4-TRIMETHYLQUINOLINE_(MONOMER)

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                    FEMALE MICE
                       FIRST TERMINAL SACRIFICE AT 372 DAYS
                                                          

                          INDIVIDUAL SURVIVAL TIMES (DAYS)
 ==================================================================================
 |                          DOSE = ACET/TMQ5 MG/KG                                |
 |                       ----------------------------                             |
 |                                                                                |
 |  TOTAL 30     UNCENSORED DEATHS 1      CENSORED DEATHS 0    TERMINAL 29        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      327                                                                       |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                          DOSE = ACET/TMQ10 MG/KG                               |
 |                       ----------------------------                             |
 |                                                                                |
 |  TOTAL 30     UNCENSORED DEATHS 1      CENSORED DEATHS 0    TERMINAL 29        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      119                                                                       |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                          DOSE = ACET/TMQ25 MG/KG                               |
 |                       ----------------------------                             |
 |                                                                                |
 |  TOTAL 30     UNCENSORED DEATHS 3      CENSORED DEATHS 0    TERMINAL 27        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      183   276   369                                                           |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 ==================================================================================
                  1,2-DIHYDRO-2,2,4-TRIMETHYLQUINOLINE_(MONOMER)

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                    FEMALE MICE
                       FIRST TERMINAL SACRIFICE AT 372 DAYS
                                                          

                          INDIVIDUAL SURVIVAL TIMES (DAYS)
 ==================================================================================
 |                                DOSE = TMQ/TPA                                  |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 30     UNCENSORED DEATHS 5      CENSORED DEATHS 0    TERMINAL 25        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      201   213   221   313   326                                               |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 ==================================================================================
                  1,2-DIHYDRO-2,2,4-TRIMETHYLQUINOLINE_(MONOMER)

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                    FEMALE MICE
                       FIRST TERMINAL SACRIFICE AT 372 DAYS

 ==================================================================================
 |               KAPLAN-MEIER SURVIVAL PROBABILITY ESTIMATES (%)                  |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |    DOSE                               TIME (DAYS)                              |
 |                 90   180   270   365   425   485   545   605   665   372 (A)   |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |           |                                                                    |
 |DMBA/TMQ5  |   100.0 100.0 100.0  93.3  93.3  93.3  93.3  93.3  93.3  93.3      |
 |MG/KG      |                                                                    |
 |           |                                                                    |
 |DMBA/TMQ10 |   100.0 100.0  96.7  93.3  93.3  93.3  93.3  93.3  93.3  93.3      |
 |MG/KG      |                                                                    |
 |           |                                                                    |
 |DMBA/TMQ25 |    96.7  96.7  93.3  90.0  90.0  90.0  90.0  90.0  90.0  90.0      |
 |MG/KG      |                                                                    |
 |           |                                                                    |
 |ACET/TMQ5  |   100.0 100.0 100.0  96.7  96.7  96.7  96.7  96.7  96.7  96.7      |
 |MG/KG      |                                                                    |
 |           |                                                                    |
 |ACET/TMQ10 |   100.0  96.7  96.7  96.7  96.7  96.7  96.7  96.7  96.7  96.7      |
 |MG/KG      |                                                                    |
 |           |                                                                    |
 |ACET/TMQ25 |   100.0 100.0  96.7  93.3  90.0  90.0  90.0  90.0  90.0  90.0      |
 |MG/KG      |                                                                    |
 |           |                                                                    |
 |TMQ/TPA    |   100.0 100.0  90.0  83.3  83.3  83.3  83.3  83.3  83.3  83.3      |
 |           |                                                                    |
 |           |                                                                    |
 ==================================================================================
                  1,2-DIHYDRO-2,2,4-TRIMETHYLQUINOLINE_(MONOMER)

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                    FEMALE MICE
                       FIRST TERMINAL SACRIFICE AT 372 DAYS

 ===============================================================================================================================
 |                                               SURVIVAL SUMMARY STATISTICS                                                   |
 |                                                                                                                             |
 |                                                                                                                             |
 |-----------------------------------------------------------------------------------------------------------------------------|
 |                                                                        DOSE                                                 |
 |                                                                                                                             |
 |                                DMBA/TMQ5    DMBA/TMQ10   DMBA/TMQ25   ACET/TMQ5    ACET/TMQ10   ACET/TMQ25   TMQ/TPA        |
 |                                MG/KG        MG/KG        MG/KG        MG/KG        MG/KG        MG/KG                       |
 |-----------------------------------------------------------------------------------------------------------------------------|
 |SURVIVAL AT END OF STUDY|       93.3%        93.3%        90.0%        96.7%        96.7%        90.0%        83.3%          |
 |(KAPLAN-MEIER)          |                                                                                                    |
 |                        |                                                                                                    |
 |SIGNIFICANCE (B)        |       P=0.116       -----        -----        -----        -----        -----       P=0.376        |
 |(LIFE TABLE)            |                                                                                                    |
 |                        |                                                                                                    |
 |MEAN DAY OF             |       350.5        276.5        219.7        327.0        119.0        276.0        254.8          |
 |NATURAL DEATHS (C)      |       ( 4.5)       (36.5)       (91.0)       (  . )       (  . )       (53.7)       (26.7)         |
 |(STANDARD ERROR)        |                                                                                                    |
 |                        |                                                                                                    |
 |MEAN LIFE SPAN (D)      |       370.6        365.6        356.8        370.5        363.6        362.4        352.5          |
 |(STANDARD ERROR)        |       ( 1.0)       ( 4.8)       (11.4)       ( 1.5)       ( 8.4)       ( 7.0)       ( 9.1)         |
 |                        |                                                                                                    |
 ===============================================================================================================================
                                    

       (A)  FIRST TERMINAL SACRIFICE
       (B)  THE FIRST ENTRY IS THE TREND TEST (TARONE, 1975) RESULT.
            SUBSEQUENT ENTRIES ARE THE RESULTS OF PAIRWISE TESTS
            (COX,1972).  NEGATIVE TRENDS ARE INDICATED BY "N".
       (C)  MEAN OF ALL UNCENSORED DEATHS PRIOR TO TERMINAL SACRIFICE
       (D)  MEAN OF ALL DEATHS (UNCENSORED, CENSORED, TERMINAL SACRIFICE)
                                           1,2-DIHYDRO-2,2,4-TRIMETHYLQUINOLINE_(MONOMER)
                                                    KAPLAN-MEIER SURVIVAL CURVES
                                                        DMBA/TMQ5 MG/KG  = 1
                                                        DMBA/TMQ10 MG/KG = 2
                                                        DMBA/TMQ25 MG/KG = 3
                                                        ACET/TMQ5 MG/KG  = 4
                                                        ACET/TMQ10 MG/KG = 5
                                                        ACET/TMQ25 MG/KG = 6
                                                        TMQ/TPA          = 7

----------------------------------------------------- SPECIES=MICE SEX=FEMALE ------------------------------------------------------

                                         Plot of SURVIVAL*WEEK.  Symbol is value of DOSE.

         |
         |
  100.00 +1                 3                  5                     6    7            2                         4      1
         |
P        |
E        |
R  96.67 +                  3                  5                     6    7   7        2          6          2   4      1 1      4
C        |
E        |
N        |
T  93.33 +                                                                    7 7      3          6          2            1    3 1
         |
S        |
U        |
R  90.00 +                                                                      7                            7                 3 3
V        |
I        |
V        |
A  86.67 +                                                                                                   7   7
L        |
         |
         |
   83.33 +                                                                                                       7               7
         |
         -+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+
          0          5         10         15         20         25         30         35         40         45         50         55

                                                                     WEEK

NOTE: 12 obs hidden.
NTP                                         STATISTICAL ANALYSIS OF SURVIVAL DATA                              REPORT: PEIRPT11    
LAB: TSI Mason Research                      1,2-DIHYDRO-2,2,4-TRIMETHYLQUINOLINE_(MONOMER)                    DATE: 04/05/96      
EXPERIMENT: 05069 TEST: 06                                                                                     TIME: 06:12:38      
TEST TYPE:  INIT/PROMOT                         CAGES FROM 0000 TO LAST CAGE                                                       
CONT: N01-ES-95256                              ROUTE: DERMAL,SOLUTION                                         NTP C#: 60902B      
PATHOLOGIST: M.E.P. GOAD                                                                                       CAS: 147-47-7       
                  1,2-DIHYDRO-2,2,4-TRIMETHYLQUINOLINE_(MONOMER)

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                    FEMALE MICE
                       FIRST TERMINAL SACRIFICE AT 372 DAYS
                                                          

                          INDIVIDUAL SURVIVAL TIMES (DAYS)
 ==================================================================================
 |                                DOSE = DMBA/TPA                                 |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 30     UNCENSORED DEATHS 21     CENSORED DEATHS 0    TERMINAL 9         |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      228   228   231   275   281   281   306   311   313   317                 |
 |      319   323   324   337   341   344   345   347   347   352                 |
 |      355                                                                       |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                          DOSE = DMBA/   ACETONE                                |
 |                       ----------------------------                             |
 |                                                                                |
 |  TOTAL 30     UNCENSORED DEATHS 3      CENSORED DEATHS 0    TERMINAL 27        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      212   281   339                                                           |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                DOSE = ACETONE/TPA                              |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 30     UNCENSORED DEATHS 5      CENSORED DEATHS 0    TERMINAL 25        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      82    275   313   332   364                                               |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 ==================================================================================
                  1,2-DIHYDRO-2,2,4-TRIMETHYLQUINOLINE_(MONOMER)

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                    FEMALE MICE
                       FIRST TERMINAL SACRIFICE AT 372 DAYS
                                                          

                          INDIVIDUAL SURVIVAL TIMES (DAYS)
 ==================================================================================
 |                          DOSE = ACETONE/ACETONE                                |
 |                       ----------------------------                             |
 |                                                                                |
 |  TOTAL 30     UNCENSORED DEATHS 2      CENSORED DEATHS 0    TERMINAL 28        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      334   365                                                                 |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 ==================================================================================
                  1,2-DIHYDRO-2,2,4-TRIMETHYLQUINOLINE_(MONOMER)

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                    FEMALE MICE
                       FIRST TERMINAL SACRIFICE AT 372 DAYS

 ==================================================================================
 |               KAPLAN-MEIER SURVIVAL PROBABILITY ESTIMATES (%)                  |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |    DOSE                               TIME (DAYS)                              |
 |                 90   180   270   365   425   485   545   605   665   372 (A)   |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |           |                                                                    |
 |DMBA/TPA   |   100.0 100.0  90.0  30.0  30.0  30.0  30.0  30.0  30.0  30.0      |
 |           |                                                                    |
 |           |                                                                    |
 |DMBA/      |   100.0 100.0  96.7  90.0  90.0  90.0  90.0  90.0  90.0  90.0      |
 |ACETONE    |                                                                    |
 |           |                                                                    |
 |ACETONE/TPA|    96.7  96.7  96.7  83.3  83.3  83.3  83.3  83.3  83.3  83.3      |
 |           |                                                                    |
 |           |                                                                    |
 |ACETONE/ACE|ONE100.0 100.0 100.0  93.3  93.3  93.3  93.3  93.3  93.3  93.3      |
 |A          |                                                                    |
 |           |                                                                    |
 ==================================================================================
 ==================================================================================
 |                           SURVIVAL SUMMARY STATISTICS                          |
 |                                                                                |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                                  DOSE                          |
 |                                                                                |
 |                                DMBA/TPA     DMBA/        ACETONE/TPA  ACETONE/A|ETONE
 |                                             ACETONE                   A        |
 |--------------------------------------------------------------------------------|
 |SURVIVAL AT END OF STUDY|       30.0%        90.0%        83.3%        93.3%    |
 |(KAPLAN-MEIER)          |                                                       |
 |                        |                                                       |
 |SIGNIFICANCE (B)        |        -----        -----        -----        -----   |
 |(LIFE TABLE)            |                                                       |
 |                        |                                                       |
 |MEAN DAY OF             |       309.8        277.3        273.2        349.5    |
 |NATURAL DEATHS (C)      |       ( 9.0)       (36.7)       (49.9)       (15.5)   |
 |(STANDARD ERROR)        |                                                       |
 |                        |                                                       |
 |MEAN LIFE SPAN (D)      |       328.4        362.5        355.5        370.5    |
 |(STANDARD ERROR)        |       ( 8.2)       ( 6.1)       (10.2)       ( 1.3)   |
 |                        |                                                       |
 ==================================================================================
                                    

       (A)  FIRST TERMINAL SACRIFICE
       (B)  THE FIRST ENTRY IS THE TREND TEST (TARONE, 1975) RESULT.
            SUBSEQUENT ENTRIES ARE THE RESULTS OF PAIRWISE TESTS
            (COX,1972).  NEGATIVE TRENDS ARE INDICATED BY "N".
       (C)  MEAN OF ALL UNCENSORED DEATHS PRIOR TO TERMINAL SACRIFICE
       (D)  MEAN OF ALL DEATHS (UNCENSORED, CENSORED, TERMINAL SACRIFICE)
                                           1,2-DIHYDRO-2,2,4-TRIMETHYLQUINOLINE_(MONOMER)
                                                    KAPLAN-MEIER SURVIVAL CURVES
                                                        DMBA/TPA         = 1
                                                        DMBA/   ACETONE  = 2
                                                        ACETONE/TPA      = 3
                                                        ACETONE/ACETONE  = 4

----------------------------------------------------- SPECIES=MICE SEX=FEMALE ------------------------------------------------------

                                         Plot of SURVIVAL*WEEK.  Symbol is value of DOSE.

      |
      |
  100 + 1                         3                                         2    1                                4
      |                           3                                         2                   3 2               4          4
      |
      |                                                                                         3 2        3        2        4 4
   90 +                                                                          1              1          3      3 2          2
      |                                                                                         1 1               3       3
P     |
E     |                                                                                                                   3    3
R  80 +                                                                                           1      1
C     |                                                                                                  1 1
E     |
N     |
T  70 +                                                                                                    1 1
      |
S     |
U     |                                                                                                      1 1
R  60 +
V     |                                                                                                        1    1
I     |
V     |
A  50 +                                                                                                             1 1
L     |
      |
      |
   40 +
      |                                                                                                               1 1
      |
      |
   30 +                                                                                                                 1      1
      |
      --+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+--
        0          5         10         15         20         25         30         35         40         45         50         55

                                                                   WEEK

NOTE: 3 obs hidden.
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.