National Toxicology Program

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TDMS Study 05111-01 Pathology Tables

NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: Mason                                               1,2,3-TRICHLOROPROPANE                                DATE: 09/14/94       
EXPERIMENT: 05111 TEST: 01                                                                                     TIME: 05:25:07       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                   PAGE:       1        
CONT: N01-ES-45048          ROUTE: GAVAGE                                                                      NTP C#: 60220B       
PATHOLOGIST: K. MURTHY                       S. EUSTIS                                                         CAS: 96-18-4         
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               REASONS FOR REMOVAL:    ALL                                                                                          
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
               REMOVAL DATE RANGE:     ALL                                                                                          
                                                                                                                                    
               TREATMENT GROUPS:       INCLUDE ALL                                                                                  
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08    
LAB: Mason                                               1,2,3-TRICHLOROPROPANE                                DATE: 09/14/94      
EXPERIMENT: 05111 TEST: 01                                                                                     TIME: 05:25:07      
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                       
CONT: N01-ES-45048          ROUTE: GAVAGE                                                                      NTP C#: 60220B      
PATHOLOGIST: K. MURTHY                       S. EUSTIS                                                         CAS: 96-18-4        
 
 
 
 
 
                                                    RATS(FISCHER344)
                                              ----------------------------
             FOR ALL DOSES THE TUMOR RATES IN THE FOLLOWING TISSUES/ORGANS ARE BASED ON NUMBER OF TISSUES EXAMINED.
             IN OTHER TISSUES/ORGANS, RATES ARE BASED ON NUMBER OF ANIMALS NECROPSIED.
 
 
                                                    ----------------
 
                                                    Adrenal Gland
                                                    Bone Marrow
                                                    Brain
                                                    Clitoral Gland
                                                    Epididymis
                                                    Heart
                                                    Islets, Pancreatic
                                                    Kidney
                                                    Liver
                                                    Lung
                                                    Nose
                                                    Ovary
                                                    Pancreas
                                                    Parathyroid Gland
                                                    Pituitary Gland
                                                    Preputial Gland
                                                    Prostate
                                                    Salivary Glands
                                                    Spleen
                                                    Testes
                                                    Thymus
                                                    Thyroid Gland
                                                    Urinary Bladder
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: Mason                                               1,2,3-TRICHLOROPROPANE                                DATE: 09/14/94       
EXPERIMENT: 05111 TEST: 01                                                                                     TIME: 05:25:07       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                        
CONT: N01-ES-45048          ROUTE: GAVAGE                                                                      NTP C#: 60220B       
PATHOLOGIST: K. MURTHY                       S. EUSTIS                                                         CAS: 96-18-4         
 
 
 
 
 
                                     SUMMARY OF STATISTICALLY SIGNIFICANT (P<=.05) RESULTS
                                     IN THE ANALYSIS OF THE STUDY OF 1,2,3-TRICHLOROPROPANE
 
              ====================================================================================================
 
                                                           MALE RATS
                                                          -------------
                                 ORGAN                               MORPHOLOGY
                                -------                             ------------
 
                             Adrenal Medulla               Pheochromocytoma Benign
                                                           Pheochromocytoma: Benign, Complex, Malignant, NOS
                             Clitoral/Preputial Gland      Adenoma
                                                           Carcinoma
                                                           Carcinoma or Adenoma
                             Intestine Small               Carcinoma
                             Islets, Pancreatic            Adenoma
                                                           Carcinoma or Adenoma
                             Kidney: Renal Tubule          Adenoma
                                                           Carcinoma or Adenoma
                             Liver                         Hepatocellular Adenoma
                                                           Hepatocellular Carcinoma or Hepatocellular Adenoma
                                                           Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma
                             Lung                          Alveolar/Bronchiolar Adenoma
                                                           Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma
                             Oral Cavity (Oral Mucosa, Tongue, Pharynx, Tooth, Lip)
                                                           Papilloma Squamous or Papilloma
                                                           Squamous Cell Carcinoma
                                                           Squamous Cell Carcinoma, Papilloma Squamous, or Papilloma
                             Pancreas                      Adenoma
                                                           Carcinoma or Adenoma
                             Pharynx                       Squamous Cell Carcinoma
                                                           Squamous Cell Papilloma
                             Pituitary Gland: Pars Distalis or Unspecified Site
                                                           Adenoma
                             Skin                          Keratoacanthoma
                                                           Squamous Cell Carcinoma, Papilloma Squamous, or Papilloma
                                                           Squamous Cell Papilloma
                                                           Trichoepithelioma, Kertatocanthoma, Squamous Cell Carcinoma,
                                                            Basal Cell Carcinoma or Adenoma, Papilloma Squamous, or Papilloma
                             Skin: Subcutaneous Tissue     Fibroma
                                                           Neurofibrosarcoma, Fibrosarcoma, Sarcoma,
                                                            Neurofibroma, or Fibroma
                             Stomach, Forestomach          Squamous Cell Carcinoma
                                                           Squamous Cell Carcinoma or Papilloma Squamous
                                                           Squamous Cell Papilloma
                             Testes                        Adenoma
                             Thyroid Gland: C-Cell         Adenoma
                                                           Carcinoma or Adenoma
                             Thyroid Gland: Follicular Cell
                                                           Adenoma
                                                           Carcinoma or Adenoma
                             Tongue                        Squamous Cell Carcinoma
                                                           Squamous Cell Carcinoma or Papilloma Squamous
                                                           Squamous Cell Papilloma
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: Mason                                               1,2,3-TRICHLOROPROPANE                                DATE: 09/14/94       
EXPERIMENT: 05111 TEST: 01                                                                                     TIME: 05:25:07       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                        
CONT: N01-ES-45048          ROUTE: GAVAGE                                                                      NTP C#: 60220B       
PATHOLOGIST: K. MURTHY                       S. EUSTIS                                                         CAS: 96-18-4         
 
                             Zymbal's Gland                Carcinoma
                             All Organs                    Hemangioma
                                                           Hemangiosarcoma or Hemangioma
                                                           Leukemia: Lymphocytic, Monocytic, Mononuclear,
                                                            or Undifferentiated
                                                           Mesothelioma Benign, Malignant, NOS
                                                           Benign Tumors
                                                           Malignant Tumors
                                                           Malignant and Benign Tumors
 
              ====================================================================================================
 
                                                           FEMALE RATS
                                                          -------------
                                 ORGAN                               MORPHOLOGY
                                -------                             ------------
 
                             Adrenal Medulla               Pheochromocytoma Benign
                                                           Pheochromocytoma: Benign, Complex, Malignant, NOS
                             Clitoral/Preputial Gland      Adenoma
                                                           Carcinoma
                                                           Carcinoma or Adenoma
                             Intestine Small               Carcinoma
                             Mammary Gland                 Adenoma
                                                           Carcinoma
                                                           Carcinoma or Adenoma
                                                           Fibroadenoma
                                                           Fibroma, Fibroadenoma or Adenoma
                                                           Fibroma, Fibroadenoma, Carcinoma, or Adenoma
                             Oral Cavity (Oral Mucosa, Tongue, Pharynx, Tooth, Lip)
                                                           Papilloma Squamous or Papilloma
                                                           Squamous Cell Carcinoma
                                                           Squamous Cell Carcinoma, Papilloma Squamous, or Papilloma
                             Pancreas                      Adenoma
                                                           Carcinoma or Adenoma
                             Pharynx                       Squamous Cell Carcinoma
                                                           Squamous Cell Papilloma
                             Pituitary Gland: Pars Distalis or Unspecified Site
                                                           Adenoma
                             Stomach, Forestomach          Squamous Cell Carcinoma
                                                           Squamous Cell Carcinoma or Papilloma Squamous
                                                           Squamous Cell Papilloma
                             Thyroid Gland: C-Cell         Adenoma
                                                           Carcinoma or Adenoma
                             Thyroid Gland: Follicular Cell
                                                           Adenoma
                                                           Carcinoma or Adenoma
                             Tongue                        Squamous Cell Carcinoma
                                                           Squamous Cell Carcinoma or Papilloma Squamous
                                                           Squamous Cell Papilloma
                             Uterus                        Adenoma
                                                           Polyp Stromal
                                                           Sarcoma Stromal or Polyp Stromal
                             Zymbal's Gland                Carcinoma
                             All Organs                    Hemangiosarcoma or Hemangioma
                                                           Leukemia: Lymphocytic, Monocytic, Mononuclear,
                                                            or Undifferentiated
                                                           Benign Tumors
                                                           Malignant Tumors
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: Mason                                               1,2,3-TRICHLOROPROPANE                                DATE: 09/14/94       
EXPERIMENT: 05111 TEST: 01                                                                                     TIME: 05:25:07       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                        
CONT: N01-ES-45048          ROUTE: GAVAGE                                                                      NTP C#: 60220B       
PATHOLOGIST: K. MURTHY                       S. EUSTIS                                                         CAS: 96-18-4         
 
                                                           Malignant and Benign Tumors
 
              ====================================================================================================
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE   1
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Adrenal Medulla                                                                                                                  |
|     Pheochromocytoma Benign                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 10/60 (17%)   7/60 (12%)  13/58 (22%)   0/59 (0%)  |  5/59 (8%)    2/57 (4%)    1/60 (2%)    0/58 (0%)  |
|ADJUSTED (b)           |  27.6%        19.7%        54.1%         0.0%      |  16.7%         6.5%        12.5%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  8/34 (24%)   5/32 (16%)   5/14 (36%)   0/0        |  5/30 (17%)   1/29 (3%)    1/8 (13%)    0/0        |
|FIRST INCIDENCE (DAYS) |   663          600          596          ---       |   736 (T)      725          736 (T)      ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.009 **   P=0.322N     P=0.013 *      (e)      |  P=0.757N     P=0.226N     P=0.601N       (e)      |
|INCIDENTAL TUMOR       |  P=0.106      P=0.233N     P=0.169        (e)      |  P=0.686N     P=0.222N     P=0.601N       (e)      |
|LOGISTIC REGRESSION    |  P=0.196      P=0.256N     P=0.076      P=0.842N   |  P=0.702N     P=0.210N     P=0.601N       (e)      |
|COCHRAN-ARMITAGE       |  P=0.004N**                                        |  P=0.032N*                                         |
|FISHER EXACT           |               P=0.301N     P=0.289      P<0.001N** |               P=0.234N     P=0.100N     P=0.030N*  |
|=================================================================================================================================|
|                                                                                                                                 |
|Adrenal Medulla                                                                                                                  |
|     Pheochromocytoma Malignant                                                                                                  |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/60 (2%)    1/60 (2%)    2/58 (3%)    0/59 (0%)  |  2/59 (3%)    0/57 (0%)    0/60 (0%)    0/58 (0%)  |
|ADJUSTED (b)           |   2.9%         2.5%        14.3%         0.0%      |   6.7%         0.0%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  1/34 (3%)    0/32 (0%)    2/14 (14%)   0/0        |  2/30 (7%)    0/29 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      685          729 (T)      ---       |   736 (T)      ---          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.463      P=0.752N     P=0.209        (e)      |  P=0.876N     P=0.245N     P=0.555N       (e)      |
|INCIDENTAL TUMOR       |  P=0.603      P=0.676N     P=0.209        (e)      |  P=0.876N     P=0.245N     P=0.555N       (e)      |
|LOGISTIC REGRESSION    |  P=0.583      P=0.752N     P=0.209        (e)      |  P=0.876N     P=0.245N     P=0.555N       (e)      |
|COCHRAN-ARMITAGE       |  P=0.338N                                          |  P=0.243N                                          |
|FISHER EXACT           |               P=0.752N     P=0.487      P=0.504N   |               P=0.257N     P=0.244N     P=0.252N   |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE   2
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Adrenal Medulla                                                                                                                  |
|     Pheochromocytoma: Benign, Complex, Malignant, NOS                                                                           |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 11/60 (18%)   8/60 (13%)  14/58 (24%)   0/59 (0%)  |  7/59 (12%)   2/57 (4%)    1/60 (2%)    0/58 (0%)  |
|ADJUSTED (b)           |  30.4%        21.7%        59.2%         0.0%      |  23.3%         6.5%        12.5%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  9/34 (26%)   5/32 (16%)   6/14 (43%)   0/0        |  7/30 (23%)   1/29 (3%)    1/8 (13%)    0/0        |
|FIRST INCIDENCE (DAYS) |   663          600          596          ---       |   736 (T)      725          736 (T)      ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.007 **   P=0.327N     P=0.009 **     (e)      |  P=0.540N     P=0.085N     P=0.430N       (e)      |
|INCIDENTAL TUMOR       |  P=0.103      P=0.212N     P=0.124        (e)      |  P=0.472N     P=0.083N     P=0.430N       (e)      |
|LOGISTIC REGRESSION    |  P=0.174      P=0.261N     P=0.065      P=0.853N   |  P=0.476N     P=0.075N     P=0.430N       (e)      |
|COCHRAN-ARMITAGE       |  P=0.002N**                                        |  P=0.012N*                                         |
|FISHER EXACT           |               P=0.309N     P=0.293      P<0.001N** |               P=0.090N     P=0.029N*    P=0.007N** |
|=================================================================================================================================|
|                                                                                                                                 |
|Clitoral/Preputial Gland                                                                                                         |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  5/59 (8%)    3/57 (5%)    6/59 (10%)  11/58 (19%) |  5/56 (9%)   11/56 (20%)  14/58 (24%)  12/59 (20%) |
|ADJUSTED (b)           |  13.6%         9.6%        25.6%        55.5%      |  17.0%        31.5%        83.1%        49.4%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    1/10 (10%)   0/8 (0%)   |  0/10 (0%)    1/10 (10%)   1/8 (13%)    2/8 (25%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          3/9 (33%)  |                                                    |
|TERMINAL (d)           |  4/34 (12%)   2/29 (7%)    2/14 (14%)   0/0        |  4/28 (14%)   7/29 (24%)   6/8 (75%)    0/0        |
|FIRST INCIDENCE (DAYS) |   506          703          450 (I)      459       |   717          465 (I)      463 (I)      310       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.421N     P=0.154      P<0.001 ** |  P<0.001 **   P=0.105      P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P=0.256      P=0.510N     P=0.388      P=0.466N   |  P=0.004 **   P=0.104      P<0.001 **   P=0.123    |
|LOGISTIC REGRESSION    |  P=0.002 **   P=0.363N     P=0.404      P=0.023 *  |  P<0.001 **   P=0.098      P=0.001 **   P=0.030 *  |
|COCHRAN-ARMITAGE       |  P=0.014 *                                         |  P=0.187                                           |
|FISHER EXACT           |               P=0.378N     P=0.500      P=0.083    |               P=0.088      P=0.026 *    P=0.071    |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE   3
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Clitoral/Preputial Gland                                                                                                         |
|     Carcinoma                                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/59 (0%)    3/57 (5%)    3/59 (5%)    6/58 (10%) |  0/56 (0%)    0/56 (0%)    4/58 (7%)    6/59 (10%) |
|ADJUSTED (b)           |   0.0%         7.4%        10.9%        15.9%      |   0.0%         0.0%         7.5%        15.5%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    1/8 (13%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  0/34 (0%)    0/29 (0%)    1/14 (7%)    0/0        |  0/28 (0%)    0/29 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   ---          654          404          382       |   ---          ---          434          331       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.143      P=0.070      P=0.005 ** |  P<0.001 **     (e)        P=0.059      P=0.004 ** |
|INCIDENTAL TUMOR       |  P=0.434      P=0.302      P=0.212      P=0.326    |  P=0.451N       (e)        P=0.403      P=0.747    |
|LOGISTIC REGRESSION    |  P=0.103      P=0.118      P=0.152      P=0.164    |  P=0.404        (e)        P=0.176      P=0.246    |
|COCHRAN-ARMITAGE       |  P=0.021 *                                         |  P=0.003 **                                        |
|FISHER EXACT           |               P=0.115      P=0.122      P=0.013 *  |                 (e)        P=0.064      P=0.016 *  |
|=================================================================================================================================|
|                                                                                                                                 |
|Clitoral/Preputial Gland                                                                                                         |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  5/59 (8%)    6/57 (11%)   9/59 (15%)  17/58 (29%) |  5/56 (9%)   11/56 (20%)  18/58 (31%)  17/59 (29%) |
|ADJUSTED (b)           |  13.6%        16.4%        34.6%        62.7%      |  17.0%        31.5%        84.4%        56.2%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    1/10 (10%)   1/8 (13%)  |  0/10 (0%)    1/10 (10%)   1/8 (13%)    2/8 (25%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          3/9 (33%)  |                                                    |
|TERMINAL (d)           |  4/34 (12%)   2/29 (7%)    3/14 (21%)   0/0        |  4/28 (14%)   7/29 (24%)   6/8 (75%)    0/0        |
|FIRST INCIDENCE (DAYS) |   506          654          404          382       |   717          465 (I)      434          310       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.463      P=0.028 *    P<0.001 ** |  P<0.001 **   P=0.105      P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P=0.179      P=0.524      P=0.176      P=0.616    |  P=0.023 *    P=0.104      P<0.001 **   P=0.097    |
|LOGISTIC REGRESSION    |  P<0.001 **   P=0.491      P=0.163      P=0.007 ** |  P<0.001 **   P=0.098      P<0.001 **   P=0.013 *  |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P=0.020 *                                         |
|FISHER EXACT           |               P=0.476      P=0.197      P=0.004 ** |               P=0.088      P=0.003 **   P=0.006 ** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE   4
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Ear                                                                                                                              |
|     Sarcoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    1/60 (2%)    2/59 (3%)    0/60 (0%)  |  0/60 (0%)    0/59 (0%)    1/60 (2%)    0/60 (0%)  |
|ADJUSTED (b)           |   0.0%         3.1%        10.9%         0.0%      |   0.0%         0.0%         2.3%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  0/34 (0%)    1/32 (3%)    1/14 (7%)    0/0        |  0/31 (0%)    0/30 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   ---          729 (T)      669          ---       |   ---          ---          510          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.291      P=0.488      P=0.117        (e)      |  P=0.842        (e)        P=0.482        (e)      |
|INCIDENTAL TUMOR       |  P=0.435      P=0.488      P=0.241        (e)      |  P=0.537N       (e)        P=0.761        (e)      |
|LOGISTIC REGRESSION    |  P=0.474      P=0.488      P=0.168        (e)      |  P=0.771N       (e)        P=0.593        (e)      |
|COCHRAN-ARMITAGE       |  P=0.515N                                          |  P=0.785N                                          |
|FISHER EXACT           |               P=0.500      P=0.244        (e)      |                 (e)        P=0.500        (e)      |
|=================================================================================================================================|
|                                                                                                                                 |
|Intestine Small: Jejunum                                                                                                         |
|     Carcinoma                                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/60 (0%)    1/59 (2%)    1/60 (2%)  |  0/60 (0%)    0/59 (0%)    1/60 (2%)    2/60 (3%)  |
|ADJUSTED (b)           |   0.0%         0.0%         2.7%         4.3%      |   0.0%         0.0%         7.1%        15.3%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     1/8 (13%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  0/34 (0%)    0/32 (0%)    0/14 (0%)    0/0        |  0/31 (0%)    0/30 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          590          487       |   ---          ---          699          407       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.041 *      (e)        P=0.457      P=0.353    |  P=0.003 **     (e)        P=0.318      P=0.115    |
|INCIDENTAL TUMOR       |  P=0.457        (e)        P=0.443      P=0.983    |  P=0.055        (e)        P=0.500      P=0.377    |
|LOGISTIC REGRESSION    |  P=0.408        (e)        P=0.516      P=0.677    |  P=0.071        (e)        P=0.383      P=0.348    |
|COCHRAN-ARMITAGE       |  P=0.303                                           |  P=0.085                                           |
|FISHER EXACT           |                 (e)        P=0.496      P=0.500    |                 (e)        P=0.500      P=0.248    |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE   5
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Islets, Pancreatic                                                                                                               |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  9/60 (15%)   4/60 (7%)    3/59 (5%)    1/60 (2%)  |  2/60 (3%)    2/58 (3%)    0/60 (0%)    1/60 (2%)  |
|ADJUSTED (b)           |  24.4%        11.3%        17.3%         3.4%      |   6.5%         6.7%         0.0%         4.2%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  7/34 (21%)   3/32 (9%)    2/14 (14%)   0/0        |  2/31 (6%)    2/30 (7%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   614          603          660          479       |   736 (T)      736 (T)      ---          399       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.436      P=0.132N     P=0.387N     P=0.395    |  P=0.212      P=0.684      P=0.563N     P=0.318    |
|INCIDENTAL TUMOR       |  P=0.541N     P=0.127N     P=0.225N     P=0.983    |  P=0.592      P=0.684      P=0.563N     P=0.860    |
|LOGISTIC REGRESSION    |  P=0.392N     P=0.100N     P=0.175N     P=0.712N   |  P=0.528      P=0.684      P=0.563N     P=0.669    |
|COCHRAN-ARMITAGE       |  P=0.015N*                                         |  P=0.378N                                          |
|FISHER EXACT           |               P=0.120N     P=0.067N     P=0.008N** |               P=0.678      P=0.248N     P=0.500N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Islets, Pancreatic                                                                                                               |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 10/60 (17%)   4/60 (7%)    3/59 (5%)    1/60 (2%)  |  3/60 (5%)    3/58 (5%)    0/60 (0%)    1/60 (2%)  |
|ADJUSTED (b)           |  27.2%        11.3%        17.3%         3.4%      |   9.4%         9.3%         0.0%         4.2%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  8/34 (24%)   3/32 (9%)    2/14 (14%)   0/0        |  2/31 (6%)    2/30 (7%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   614          603          660          479       |   717          696          ---          399       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.504      P=0.087N     P=0.320N     P=0.395    |  P=0.305      P=0.653      P=0.408N     P=0.318    |
|INCIDENTAL TUMOR       |  P=0.471N     P=0.083N     P=0.177N     P=0.983    |  P=0.724      P=0.642N     P=0.293N     P=0.860    |
|LOGISTIC REGRESSION    |  P=0.343N     P=0.061N     P=0.126N     P=0.724N   |  P=0.649      P=0.652N     P=0.331N     P=0.667    |
|COCHRAN-ARMITAGE       |  P=0.010N**                                        |  P=0.191N                                          |
|FISHER EXACT           |               P=0.077N     P=0.040N*    P=0.004N** |               P=0.644      P=0.122N     P=0.309N   |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE   6
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney: Renal Tubule                                                                                                             |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    2/60 (3%)   20/59 (34%)  26/60 (43%) |  0/60 (0%)    0/57 (0%)    0/60 (0%)    0/59 (0%)  |
|ADJUSTED (b)           |   0.0%         6.3%        76.3%        85.0%      |   0.0%         0.0%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    5/8 (63%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          6/9 (67%)  |                                                    |
|TERMINAL (d)           |  0/34 (0%)    2/32 (6%)    8/14 (57%)   0/0        |  0/31 (0%)    0/30 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   ---          729 (T)      660          423       |   ---          ---          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.225      P<0.001 **   P<0.001 ** |    (e)          (e)          (e)          (e)      |
|INCIDENTAL TUMOR       |  P<0.001 **   P=0.225      P<0.001 **   P=0.006 ** |    (e)          (e)          (e)          (e)      |
|LOGISTIC REGRESSION    |  P<0.001 **   P=0.225      P<0.001 **   P<0.001 ** |    (e)          (e)          (e)          (e)      |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |    (e)                                             |
|FISHER EXACT           |               P=0.248      P<0.001 **   P<0.001 ** |                 (e)          (e)          (e)      |
|=================================================================================================================================|
|                                                                                                                                 |
|Kidney: Renal Tubule                                                                                                             |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    2/60 (3%)   20/59 (34%)  26/60 (43%) |  0/60 (0%)    0/57 (0%)    0/60 (0%)    1/59 (2%)  |
|ADJUSTED (b)           |   0.0%         6.3%        76.3%        85.0%      |   0.0%         0.0%         0.0%         5.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    5/8 (63%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          6/9 (67%)  |                                                    |
|TERMINAL (d)           |  0/34 (0%)    2/32 (6%)    8/14 (57%)   0/0        |  0/31 (0%)    0/30 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   ---          729 (T)      660          423       |   ---          ---          ---          408       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.225      P<0.001 **   P<0.001 ** |  P=0.067        (e)          (e)        P=0.282    |
|INCIDENTAL TUMOR       |  P<0.001 **   P=0.225      P<0.001 **   P=0.006 ** |  P=0.466        (e)          (e)        P=0.853    |
|LOGISTIC REGRESSION    |  P<0.001 **   P=0.225      P<0.001 **   P<0.001 ** |  P=0.474        (e)          (e)        P=0.769    |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P=0.214                                           |
|FISHER EXACT           |               P=0.248      P<0.001 **   P<0.001 ** |                 (e)          (e)        P=0.496    |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE   7
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Adenoma                                                                                                      |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/60 (2%)    1/60 (2%)    3/59 (5%)    1/60 (2%)  |  0/60 (0%)    0/59 (0%)    1/60 (2%)    0/60 (0%)  |
|ADJUSTED (b)           |   2.9%         3.1%        19.3%         9.1%      |   0.0%         0.0%        12.5%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  1/34 (3%)    1/32 (3%)    2/14 (14%)   0/0        |  0/31 (0%)    0/30 (0%)    1/8 (13%)    0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      729 (T)      709          520       |   ---          ---          736 (T)      ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.002 **   P=0.748      P=0.076      P=0.214    |  P=0.788        (e)        P=0.233        (e)      |
|INCIDENTAL TUMOR       |  P=0.204      P=0.748      P=0.166      P=0.983    |  P=0.788        (e)        P=0.233        (e)      |
|LOGISTIC REGRESSION    |  P=0.054      P=0.748      P=0.120      P=0.601    |  P=0.788        (e)        P=0.233        (e)      |
|COCHRAN-ARMITAGE       |  P=0.618N                                          |  P=0.785N                                          |
|FISHER EXACT           |               P=0.752N     P=0.303      P=0.752N   |                 (e)        P=0.500        (e)      |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma                                                                                                    |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/60 (0%)    1/59 (2%)    2/60 (3%)  |  0/60 (0%)    0/59 (0%)    0/60 (0%)    0/60 (0%)  |
|ADJUSTED (b)           |   0.0%         0.0%         4.0%        13.3%      |   0.0%         0.0%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          1/9 (11%)  |                                                    |
|TERMINAL (d)           |  0/34 (0%)    0/32 (0%)    0/14 (0%)    0/0        |  0/31 (0%)    0/30 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          669          452       |   ---          ---          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.073        (e)        P=0.427      P=0.440    |    (e)          (e)          (e)          (e)      |
|INCIDENTAL TUMOR       |  P=0.541        (e)        P=0.742      P=0.983    |    (e)          (e)          (e)          (e)      |
|LOGISTIC REGRESSION    |  P=0.117        (e)        P=0.465      P=0.381    |    (e)          (e)          (e)          (e)      |
|COCHRAN-ARMITAGE       |  P=0.084                                           |    (e)                                             |
|FISHER EXACT           |                 (e)        P=0.496      P=0.248    |                 (e)          (e)          (e)      |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE   8
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma or Hepatocellular Adenoma                                                                          |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/60 (2%)    1/60 (2%)    4/59 (7%)    3/60 (5%)  |  0/60 (0%)    0/59 (0%)    1/60 (2%)    0/60 (0%)  |
|ADJUSTED (b)           |   2.9%         3.1%        22.6%        21.2%      |   0.0%         0.0%        12.5%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          1/9 (11%)  |                                                    |
|TERMINAL (d)           |  1/34 (3%)    1/32 (3%)    2/14 (14%)   0/0        |  0/31 (0%)    0/30 (0%)    1/8 (13%)    0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      729 (T)      669          452       |   ---          ---          736 (T)      ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.748      P=0.034 *    P=0.080    |  P=0.788        (e)        P=0.233        (e)      |
|INCIDENTAL TUMOR       |  P=0.113      P=0.748      P=0.131      P=0.913    |  P=0.788        (e)        P=0.233        (e)      |
|LOGISTIC REGRESSION    |  P=0.011 *    P=0.748      P=0.073      P=0.216    |  P=0.788        (e)        P=0.233        (e)      |
|COCHRAN-ARMITAGE       |  P=0.223                                           |  P=0.785N                                          |
|FISHER EXACT           |               P=0.752N     P=0.177      P=0.309    |                 (e)        P=0.500        (e)      |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma                                                         |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/60 (2%)    1/60 (2%)    4/59 (7%)    3/60 (5%)  |  0/60 (0%)    0/59 (0%)    1/60 (2%)    0/60 (0%)  |
|ADJUSTED (b)           |   2.9%         3.1%        22.6%        21.2%      |   0.0%         0.0%        12.5%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          1/9 (11%)  |                                                    |
|TERMINAL (d)           |  1/34 (3%)    1/32 (3%)    2/14 (14%)   0/0        |  0/31 (0%)    0/30 (0%)    1/8 (13%)    0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      729 (T)      669          452       |   ---          ---          736 (T)      ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.748      P=0.034 *    P=0.080    |  P=0.788        (e)        P=0.233        (e)      |
|INCIDENTAL TUMOR       |  P=0.113      P=0.748      P=0.131      P=0.913    |  P=0.788        (e)        P=0.233        (e)      |
|LOGISTIC REGRESSION    |  P=0.011 *    P=0.748      P=0.073      P=0.216    |  P=0.788        (e)        P=0.233        (e)      |
|COCHRAN-ARMITAGE       |  P=0.223                                           |  P=0.785N                                          |
|FISHER EXACT           |               P=0.752N     P=0.177      P=0.309    |                 (e)        P=0.500        (e)      |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE   9
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Adenoma                                                                                                |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  2/60 (3%)    1/59 (2%)    2/59 (3%)    2/60 (3%)  |  2/60 (3%)    1/58 (2%)    1/59 (2%)    0/60 (0%)  |
|ADJUSTED (b)           |   5.5%         3.2%        11.2%         7.9%      |   5.6%         3.4%         2.3%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    1/8 (13%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  1/34 (3%)    1/31 (3%)    1/14 (7%)    0/0        |  1/31 (3%)    1/29 (3%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   663          729 (T)      684          452 (I)   |   645          736 (T)      510          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.007 **   P=0.510N     P=0.456      P=0.120    |  P=0.904      P=0.513N     P=0.729        (e)      |
|INCIDENTAL TUMOR       |  P=0.150      P=0.424N     P=0.667N     P=0.424    |  P=0.352N     P=0.492N     P=0.503N       (e)      |
|LOGISTIC REGRESSION    |  P=0.221      P=0.491N     P=0.575      P=0.610    |  P=0.481N     P=0.504N     P=0.536N     P=0.949N   |
|COCHRAN-ARMITAGE       |  P=0.532                                           |  P=0.192N                                          |
|FISHER EXACT           |               P=0.506N     P=0.684      P=0.691N   |               P=0.513N     P=0.506N     P=0.248N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Carcinoma                                                                                              |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/59 (0%)    2/59 (3%)    0/60 (0%)  |  0/60 (0%)    0/58 (0%)    1/59 (2%)    0/60 (0%)  |
|ADJUSTED (b)           |   0.0%         0.0%        10.0%         0.0%      |   0.0%         0.0%         2.9%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  0/34 (0%)    0/31 (0%)    1/14 (7%)    0/0        |  0/31 (0%)    0/29 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          641          ---       |   ---          ---          561          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.278        (e)        P=0.130        (e)      |  P=0.834        (e)        P=0.453        (e)      |
|INCIDENTAL TUMOR       |  P=0.282        (e)        P=0.123        (e)      |  P=0.947        (e)        P=0.718        (e)      |
|LOGISTIC REGRESSION    |  P=0.552        (e)        P=0.183        (e)      |  P=0.877N       (e)        P=0.547        (e)      |
|COCHRAN-ARMITAGE       |  P=0.695N                                          |  P=0.783N                                          |
|FISHER EXACT           |                 (e)        P=0.244        (e)      |                 (e)        P=0.496        (e)      |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  10
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma                                                              |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  2/60 (3%)    1/59 (2%)    4/59 (7%)    2/60 (3%)  |  2/60 (3%)    1/58 (2%)    2/59 (3%)    0/60 (0%)  |
|ADJUSTED (b)           |   5.5%         3.2%        20.6%         7.9%      |   5.6%         3.4%         5.2%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    1/8 (13%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  1/34 (3%)    1/31 (3%)    2/14 (14%)   0/0        |  1/31 (3%)    1/29 (3%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   663          729 (T)      641          452 (I)   |   645          736 (T)      510          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.001 **   P=0.510N     P=0.118      P=0.120    |  P=0.644      P=0.513N     P=0.467        (e)      |
|INCIDENTAL TUMOR       |  P=0.041 *    P=0.424N     P=0.274      P=0.424    |  P=0.442N     P=0.492N     P=0.615N       (e)      |
|LOGISTIC REGRESSION    |  P=0.132      P=0.491N     P=0.209      P=0.610    |  P=0.471N     P=0.504N     P=0.699      P=0.949N   |
|COCHRAN-ARMITAGE       |  P=0.547                                           |  P=0.209N                                          |
|FISHER EXACT           |               P=0.506N     P=0.332      P=0.691N   |               P=0.513N     P=0.684      P=0.248N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Mammary Gland                                                                                                                    |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/60 (0%)    0/59 (0%)    0/60 (0%)  |  1/60 (2%)    0/59 (0%)    3/60 (5%)    1/60 (2%)  |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%         0.0%      |   3.0%         0.0%        15.2%        16.7%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     1/8 (13%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  0/34 (0%)    0/32 (0%)    0/14 (0%)    0/0        |  0/31 (0%)    0/30 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          ---       |   714          ---          521          465 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |    (e)          (e)          (e)          (e)      |  P=0.022 *    P=0.506N     P=0.109      P=0.455    |
|INCIDENTAL TUMOR       |    (e)          (e)          (e)          (e)      |  P=0.373      P=0.460N     P=0.522      P=0.455    |
|LOGISTIC REGRESSION    |    (e)          (e)          (e)          (e)      |  P=0.337      P=0.497N     P=0.256      P=0.625    |
|COCHRAN-ARMITAGE       |    (e)                                             |  P=0.560                                           |
|FISHER EXACT           |                 (e)          (e)          (e)      |               P=0.504N     P=0.309      P=0.752N   |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  11
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Mammary Gland                                                                                                                    |
|     Carcinoma                                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/60 (0%)    0/59 (0%)    0/60 (0%)  |  1/60 (2%)    6/59 (10%)  12/60 (20%)  22/60 (37%) |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%         0.0%      |   1.7%        17.7%        51.7%        63.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     1/8 (13%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  0/34 (0%)    0/32 (0%)    0/14 (0%)    0/0        |  0/31 (0%)    4/30 (13%)   3/8 (38%)    0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          ---       |   450          469          424          239       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |    (e)          (e)          (e)          (e)      |  P<0.001 **   P=0.059      P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |    (e)          (e)          (e)          (e)      |  P=0.010 **   P=0.071      P=0.011 *    P=0.229    |
|LOGISTIC REGRESSION    |    (e)          (e)          (e)          (e)      |  P<0.001 **   P=0.057      P=0.003 **   P=0.014 *  |
|COCHRAN-ARMITAGE       |    (e)                                             |  P<0.001 **                                        |
|FISHER EXACT           |                 (e)          (e)          (e)      |               P=0.054      P=0.001 **   P<0.001 ** |
|=================================================================================================================================|
|                                                                                                                                 |
|Mammary Gland                                                                                                                    |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/60 (0%)    0/59 (0%)    0/60 (0%)  |  2/60 (3%)    6/59 (10%)  14/60 (23%)  23/60 (38%) |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%         0.0%      |   4.7%        17.7%        58.0%        70.4%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     2/8 (25%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  0/34 (0%)    0/32 (0%)    0/14 (0%)    0/0        |  0/31 (0%)    4/30 (13%)   3/8 (38%)    0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          ---       |   450          469          424          239       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |    (e)          (e)          (e)          (e)      |  P<0.001 **   P=0.135      P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |    (e)          (e)          (e)          (e)      |  P=0.003 **   P=0.166      P=0.016 *    P=0.123    |
|LOGISTIC REGRESSION    |    (e)          (e)          (e)          (e)      |  P<0.001 **   P=0.132      P=0.002 **   P=0.009 ** |
|COCHRAN-ARMITAGE       |    (e)                                             |  P<0.001 **                                        |
|FISHER EXACT           |                 (e)          (e)          (e)      |               P=0.131      P=0.001 **   P<0.001 ** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  12
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Mammary Gland                                                                                                                    |
|     Fibroadenoma                                                                                                                |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  2/60 (3%)    3/60 (5%)    2/59 (3%)    0/60 (0%)  | 15/60 (25%)  23/59 (39%)  20/60 (33%)   1/60 (2%)  |
|ADJUSTED (b)           |   5.9%         8.6%        14.3%         0.0%      |  40.1%        61.6%        88.2%         3.1%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  2/34 (6%)    2/32 (6%)    2/14 (14%)   0/0        | 10/31 (32%)  16/30 (53%)   6/8 (75%)    0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      685          729 (T)      ---       |   474          604          510          371       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.595      P=0.490      P=0.352        (e)      |  P<0.001 **   P=0.081      P<0.001 **   P=0.375    |
|INCIDENTAL TUMOR       |  P=0.696      P=0.554      P=0.352        (e)      |  P=0.358      P=0.059      P=0.033 *    P=0.311N   |
|LOGISTIC REGRESSION    |  P=0.701      P=0.520      P=0.352        (e)      |  P=0.249      P=0.078      P=0.016 *    P=0.306N   |
|COCHRAN-ARMITAGE       |  P=0.117N                                          |  P<0.001N**                                        |
|FISHER EXACT           |               P=0.500      P=0.684      P=0.248N   |               P=0.075      P=0.211      P<0.001N** |
|=================================================================================================================================|
|                                                                                                                                 |
|Mammary Gland                                                                                                                    |
|     Fibroma, Fibroadenoma or Adenoma                                                                                            |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  2/60 (3%)    3/60 (5%)    2/59 (3%)    0/60 (0%)  | 16/60 (27%)  23/59 (39%)  22/60 (37%)   2/60 (3%)  |
|ADJUSTED (b)           |   5.9%         8.6%        14.3%         0.0%      |  41.9%        61.6%        88.9%        19.3%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     1/8 (13%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  2/34 (6%)    2/32 (6%)    2/14 (14%)   0/0        | 10/31 (32%)  16/30 (53%)   6/8 (75%)    0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      685          729 (T)      ---       |   474          604          510          371       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.595      P=0.490      P=0.352        (e)      |  P<0.001 **   P=0.118      P<0.001 **   P=0.152    |
|INCIDENTAL TUMOR       |  P=0.696      P=0.554      P=0.352        (e)      |  P=0.269      P=0.092      P=0.038 *    P=0.681N   |
|LOGISTIC REGRESSION    |  P=0.701      P=0.520      P=0.352        (e)      |  P=0.048 *    P=0.114      P=0.012 *    P=0.524N   |
|COCHRAN-ARMITAGE       |  P=0.117N                                          |  P<0.001N**                                        |
|FISHER EXACT           |               P=0.500      P=0.684      P=0.248N   |               P=0.108      P=0.163      P<0.001N** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  13
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Mammary Gland                                                                                                                    |
|     Fibroma, Fibroadenoma, Carcinoma, or Adenoma                                                                                |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  2/60 (3%)    3/60 (5%)    2/59 (3%)    0/60 (0%)  | 17/60 (28%)  26/59 (44%)  29/60 (48%)  24/60 (40%) |
|ADJUSTED (b)           |   5.9%         8.6%        14.3%         0.0%      |  42.9%        67.8%        95.3%        71.4%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     2/8 (25%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  2/34 (6%)    2/32 (6%)    2/14 (14%)   0/0        | 10/31 (32%)  18/30 (60%)   7/8 (88%)    0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      685          729 (T)      ---       |   450          469          424          239       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.595      P=0.490      P=0.352        (e)      |  P<0.001 **   P=0.065      P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P=0.696      P=0.554      P=0.352        (e)      |  P=0.003 **   P=0.049 *    P=0.010 *    P=0.300    |
|LOGISTIC REGRESSION    |  P=0.701      P=0.520      P=0.352        (e)      |  P<0.001 **   P=0.057      P=0.002 **   P=0.078    |
|COCHRAN-ARMITAGE       |  P=0.117N                                          |  P=0.315                                           |
|FISHER EXACT           |               P=0.500      P=0.684      P=0.248N   |               P=0.055      P=0.019 *    P=0.124    |
|=================================================================================================================================|
|                                                                                                                                 |
|Oral Cavity (Oral Mucosa, Tongue, Pharynx, Tooth, Lip)                                                                           |
|     Papilloma Squamous or Papilloma                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    4/60 (7%)   10/59 (17%)  22/60 (37%) |  1/60 (2%)    5/59 (8%)   10/60 (17%)  21/60 (35%) |
|ADJUSTED (b)           |   0.0%        11.7%        39.3%        61.5%      |   3.2%        14.1%        58.7%        75.5%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    1/10 (10%)   3/8 (38%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     3/8 (38%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          2/9 (22%)  |                                                    |
|TERMINAL (d)           |  0/34 (0%)    2/32 (6%)    3/14 (21%)   0/0        |  1/31 (3%)    2/30 (7%)    4/8 (50%)    0/0        |
|FIRST INCIDENCE (DAYS) |   ---          694          452 (I)      337       |   736 (T)      664          405          233       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.062      P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.112      P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P<0.001 **   P=0.117      P=0.004 **   P=0.028 *  |  P<0.001 **   P=0.122      P=0.001 **   P=0.010 ** |
|LOGISTIC REGRESSION    |  P<0.001 **   P=0.069      P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.106      P=0.003 **   P<0.001 ** |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P=0.059      P<0.001 **   P<0.001 ** |               P=0.100      P=0.004 **   P<0.001 ** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  14
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Oral Cavity (Oral Mucosa, Tongue, Pharynx, Tooth, Lip)                                                                           |
|     Squamous Cell Carcinoma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/60 (2%)    0/60 (0%)   11/59 (19%)  25/60 (42%) |  0/60 (0%)    1/59 (2%)   21/60 (35%)  23/60 (38%) |
|ADJUSTED (b)           |   2.9%         0.0%        47.6%        65.6%      |   0.0%         3.3%        72.5%        73.9%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     2/8 (25%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          1/9 (11%)  |                                                    |
|TERMINAL (d)           |  1/34 (3%)    0/32 (0%)    4/14 (29%)   0/0        |  0/31 (0%)    1/30 (3%)    3/8 (38%)    0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      ---          404          327       |   ---          736 (T)      513          294       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.512N     P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.493      P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P<0.001 **   P=0.512N     P=0.006 **   P=0.151    |  P<0.001 **   P=0.493      P<0.001 **   P=0.017 *  |
|LOGISTIC REGRESSION    |  P<0.001 **   P=0.512N     P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.493      P<0.001 **   P<0.001 ** |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P=0.500N     P=0.002 **   P<0.001 ** |               P=0.496      P<0.001 **   P<0.001 ** |
|=================================================================================================================================|
|                                                                                                                                 |
|Oral Cavity (Oral Mucosa, Tongue, Pharynx, Tooth, Lip)                                                                           |
|     Squamous Cell Carcinoma, Papilloma Squamous, or Papilloma                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/60 (2%)    4/60 (7%)   19/59 (32%)  43/60 (72%) |  1/60 (2%)    6/59 (10%)  28/60 (47%)  37/60 (62%) |
|ADJUSTED (b)           |   2.9%        11.7%        66.4%        85.6%      |   3.2%        17.2%        90.3%        91.7%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    1/10 (10%)   3/8 (38%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     5/8 (63%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          3/9 (33%)  |                                                    |
|TERMINAL (d)           |  1/34 (3%)    2/32 (6%)    6/14 (43%)   0/0        |  1/31 (3%)    3/30 (10%)   6/8 (75%)    0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      694          404          327       |   736 (T)      664          405          233       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.173      P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.064      P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P<0.001 **   P=0.274      P<0.001 **   P=0.001 ** |  P<0.001 **   P=0.069      P<0.001 **   P<0.001 ** |
|LOGISTIC REGRESSION    |  P<0.001 **   P=0.192      P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.061      P<0.001 **   P<0.001 ** |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P=0.182      P<0.001 **   P<0.001 ** |               P=0.054      P<0.001 **   P<0.001 ** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  15
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Pancreas                                                                                                                         |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  5/60 (8%)   21/60 (35%)  37/59 (63%)  31/60 (52%) |  0/60 (0%)    0/59 (0%)    2/60 (3%)    0/60 (0%)  |
|ADJUSTED (b)           |  14.7%        58.0%       100.0%        96.4%      |   0.0%         0.0%        19.2%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    1/10 (10%)   2/8 (25%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          8/9 (89%)  |                                                    |
|TERMINAL (d)           |  5/34 (15%)  17/32 (53%)  14/14 (100%)  0/0        |  0/31 (0%)    0/30 (0%)    1/8 (13%)    0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      685          450 (I)      423       |   ---          ---          701          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P=0.153        (e)        P=0.046 *      (e)      |
|INCIDENTAL TUMOR       |  P<0.001 **   P<0.001 **   P<0.001 **   P=0.049 *  |  P=0.258        (e)        P=0.108        (e)      |
|LOGISTIC REGRESSION    |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P=0.253        (e)        P=0.080        (e)      |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P=0.696N                                          |
|FISHER EXACT           |               P<0.001 **   P<0.001 **   P<0.001 ** |                 (e)        P=0.248        (e)      |
|=================================================================================================================================|
|                                                                                                                                 |
|Pancreas                                                                                                                         |
|     Carcinoma                                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/60 (0%)    2/59 (3%)    1/60 (2%)  |  0/60 (0%)    0/59 (0%)    0/60 (0%)    0/60 (0%)  |
|ADJUSTED (b)           |   0.0%         0.0%        12.9%        11.1%      |   0.0%         0.0%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          1/9 (11%)  |                                                    |
|TERMINAL (d)           |  0/34 (0%)    0/32 (0%)    1/14 (7%)    0/0        |  0/31 (0%)    0/30 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          720          534 (I)   |   ---          ---          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.218        (e)        P=0.086        (e)      |    (e)          (e)          (e)          (e)      |
|INCIDENTAL TUMOR       |  P=0.426        (e)        P=0.241        (e)      |    (e)          (e)          (e)          (e)      |
|LOGISTIC REGRESSION    |  P=0.056        (e)        P=0.113      P=0.566    |    (e)          (e)          (e)          (e)      |
|COCHRAN-ARMITAGE       |  P=0.350                                           |    (e)                                             |
|FISHER EXACT           |                 (e)        P=0.244      P=0.500    |                 (e)          (e)          (e)      |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  16
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Pancreas                                                                                                                         |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  5/60 (8%)   21/60 (35%)  37/59 (63%)  31/60 (52%) |  0/60 (0%)    0/59 (0%)    2/60 (3%)    0/60 (0%)  |
|ADJUSTED (b)           |  14.7%        58.0%       100.0%        96.4%      |   0.0%         0.0%        19.2%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    1/10 (10%)   2/8 (25%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          8/9 (89%)  |                                                    |
|TERMINAL (d)           |  5/34 (15%)  17/32 (53%)  14/14 (100%)  0/0        |  0/31 (0%)    0/30 (0%)    1/8 (13%)    0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      685          450 (I)      423       |   ---          ---          701          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P=0.153        (e)        P=0.046 *      (e)      |
|INCIDENTAL TUMOR       |  P<0.001 **   P<0.001 **   P<0.001 **   P=0.049 *  |  P=0.258        (e)        P=0.108        (e)      |
|LOGISTIC REGRESSION    |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P=0.253        (e)        P=0.080        (e)      |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P=0.696N                                          |
|FISHER EXACT           |               P<0.001 **   P<0.001 **   P<0.001 ** |                 (e)        P=0.248        (e)      |
|=================================================================================================================================|
|                                                                                                                                 |
|Pharynx                                                                                                                          |
|     Squamous Cell Carcinoma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/60 (2%)    0/60 (0%)   11/59 (19%)   7/60 (12%) |  0/60 (0%)    1/59 (2%)   10/60 (17%)  16/60 (27%) |
|ADJUSTED (b)           |   2.9%         0.0%        47.6%        21.8%      |   0.0%         3.3%        45.6%        61.5%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     1/8 (13%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  1/34 (3%)    0/32 (0%)    4/14 (29%)   0/0        |  0/31 (0%)    1/30 (3%)    1/8 (13%)    0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      ---          404          423       |   ---          736 (T)      538          330       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.512N     P<0.001 **   P=0.001 ** |  P<0.001 **   P=0.493      P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P=0.077      P=0.512N     P=0.006 **   P=0.626    |  P<0.001 **   P=0.493      P=0.008 **   P=0.058    |
|LOGISTIC REGRESSION    |  P=0.003 **   P=0.512N     P<0.001 **   P=0.114    |  P<0.001 **   P=0.493      P<0.001 **   P<0.001 ** |
|COCHRAN-ARMITAGE       |  P=0.015 *                                         |  P<0.001 **                                        |
|FISHER EXACT           |               P=0.500N     P=0.002 **   P=0.031 *  |               P=0.496      P<0.001 **   P<0.001 ** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  17
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Pharynx                                                                                                                          |
|     Squamous Cell Papilloma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    2/60 (3%)    1/59 (2%)    4/60 (7%)  |  1/60 (2%)    2/59 (3%)    5/60 (8%)    3/60 (5%)  |
|ADJUSTED (b)           |   0.0%         6.1%         4.2%        19.2%      |   3.2%         5.6%        30.2%        26.7%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    1/8 (13%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     1/8 (13%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          1/9 (11%)  |                                                    |
|TERMINAL (d)           |  0/34 (0%)    1/32 (3%)    0/14 (0%)    0/0        |  1/31 (3%)    0/30 (0%)    2/8 (25%)    0/0        |
|FIRST INCIDENCE (DAYS) |   ---          710          678          424       |   736 (T)      664          405          336       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.001 **   P=0.230      P=0.425      P=0.059    |  P<0.001 **   P=0.508      P=0.012 *    P=0.030 *  |
|INCIDENTAL TUMOR       |  P=0.124      P=0.324      P=0.742      P=0.397    |  P=0.245      P=0.543      P=0.057      P=0.377    |
|LOGISTIC REGRESSION    |  P=0.046 *    P=0.247      P=0.458      P=0.196    |  P=0.145      P=0.505      P=0.092      P=0.242    |
|COCHRAN-ARMITAGE       |  P=0.048 *                                         |  P=0.323                                           |
|FISHER EXACT           |               P=0.248      P=0.496      P=0.059    |               P=0.494      P=0.103      P=0.309    |
|=================================================================================================================================|
|                                                                                                                                 |
|Pituitary Gland: Pars Distalis or Unspecified Site                                                                               |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  9/58 (16%)  15/58 (26%)   8/59 (14%)   2/59 (3%)  | 29/60 (48%)  30/58 (52%)  12/59 (20%)   5/59 (8%)  |
|ADJUSTED (b)           |  25.1%        34.3%        35.8%        14.5%      |  70.0%        76.4%        57.1%        36.3%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    2/10 (20%)   1/10 (10%)   0/8 (0%)   |  1/10 (10%)   1/10 (10%)   0/8 (0%)     2/8 (25%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          1/9 (11%)  |                                                    |
|TERMINAL (d)           |  6/32 (19%)   6/31 (19%)   3/14 (21%)   0/0        | 19/31 (61%)  21/30 (70%)   2/8 (25%)    0/0        |
|FIRST INCIDENCE (DAYS) |   648          450 (I)      450 (I)      484       |   463 (I)      465 (I)      520          331       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.343      P=0.150      P=0.214      P=0.374    |  P=0.004 **   P=0.459      P=0.486      P=0.027 *  |
|INCIDENTAL TUMOR       |  P=0.337N     P=0.205      P=0.464N     P=0.982    |  P=0.068N     P=0.456      P=0.019N*    P=0.336N   |
|LOGISTIC REGRESSION    |  P=0.153N     P=0.139      P=0.519      P=0.615    |  P=0.300N     P=0.498      P=0.028N*    P=0.622N   |
|COCHRAN-ARMITAGE       |  P=0.003N**                                        |  P<0.001N**                                        |
|FISHER EXACT           |               P=0.126      P=0.485N     P=0.025N*  |               P=0.427      P=0.001N**   P<0.001N** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  18
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Prostate                                                                                                                         |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/58 (0%)    0/60 (0%)    2/59 (3%)    0/60 (0%)  |                                                    |
|ADJUSTED (b)           |   0.0%         0.0%        11.8%         0.0%      |                                                    |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |                                                    |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  0/32 (0%)    0/32 (0%)    1/14 (7%)    0/0        |                                                    |
|FIRST INCIDENCE (DAYS) |   ---          ---          686          ---       |                                                    |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.240        (e)        P=0.106        (e)      |                                                    |
|INCIDENTAL TUMOR       |  P=0.431        (e)        P=0.248        (e)      |                                                    |
|LOGISTIC REGRESSION    |  P=0.415        (e)        P=0.166        (e)      |                                                    |
|COCHRAN-ARMITAGE       |  P=0.693N                                          |                                                    |
|FISHER EXACT           |                 (e)        P=0.252        (e)      |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin                                                                                                                             |
|     Keratoacanthoma                                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  2/60 (3%)    2/60 (3%)    1/59 (2%)    2/60 (3%)  |  0/60 (0%)    0/59 (0%)    0/60 (0%)    0/60 (0%)  |
|ADJUSTED (b)           |   5.9%         5.7%         7.1%         8.2%      |   0.0%         0.0%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  2/34 (6%)    1/32 (3%)    1/14 (7%)    0/0        |  0/31 (0%)    0/30 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      689          729 (T)      478       |   ---          ---          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.015 *    P=0.685      P=0.687      P=0.107    |    (e)          (e)          (e)          (e)      |
|INCIDENTAL TUMOR       |  P=0.548      P=0.637N     P=0.687      P=0.913    |    (e)          (e)          (e)          (e)      |
|LOGISTIC REGRESSION    |  P=0.225      P=0.680N     P=0.687      P=0.435    |    (e)          (e)          (e)          (e)      |
|COCHRAN-ARMITAGE       |  P=0.619                                           |    (e)                                             |
|FISHER EXACT           |               P=0.691N     P=0.506N     P=0.691N   |                 (e)          (e)          (e)      |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  19
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin                                                                                                                             |
|     Squamous Cell Carcinoma, Papilloma Squamous, or Papilloma                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    2/60 (3%)    1/59 (2%)    6/60 (10%) |  1/60 (2%)    0/59 (0%)    2/60 (3%)    0/60 (0%)  |
|ADJUSTED (b)           |   0.0%         5.7%         2.8%        30.5%      |   3.2%         0.0%        16.7%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    3/8 (38%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          2/9 (22%)  |                                                    |
|TERMINAL (d)           |  0/34 (0%)    1/32 (3%)    0/14 (0%)    0/0        |  1/31 (3%)    0/30 (0%)    1/8 (13%)    0/0        |
|FIRST INCIDENCE (DAYS) |   ---          689          596          450 (I)   |   736 (T)      ---          637          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.242      P=0.455      P=0.026 *  |  P=0.373      P=0.507N     P=0.156        (e)      |
|INCIDENTAL TUMOR       |  P=0.004 **   P=0.324      P=0.443      P=0.073    |  P=0.630      P=0.507N     P=0.320        (e)      |
|LOGISTIC REGRESSION    |  P=0.014 *    P=0.248      P=0.512      P=0.073    |  P=0.585      P=0.507N     P=0.289        (e)      |
|COCHRAN-ARMITAGE       |  P=0.005 **                                        |  P=0.454N                                          |
|FISHER EXACT           |               P=0.248      P=0.496      P=0.014 *  |               P=0.504N     P=0.500      P=0.500N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin                                                                                                                             |
|     Squamous Cell Papilloma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    2/60 (3%)    0/59 (0%)    5/60 (8%)  |  0/60 (0%)    0/59 (0%)    1/60 (2%)    0/60 (0%)  |
|ADJUSTED (b)           |   0.0%         5.7%         0.0%        27.7%      |   0.0%         0.0%        12.5%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    3/8 (38%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          2/9 (22%)  |                                                    |
|TERMINAL (d)           |  0/34 (0%)    1/32 (3%)    0/14 (0%)    0/0        |  0/31 (0%)    0/30 (0%)    1/8 (13%)    0/0        |
|FIRST INCIDENCE (DAYS) |   ---          689          ---          450 (I)   |   ---          ---          736 (T)      ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.005 **   P=0.242        (e)        P=0.074    |  P=0.788        (e)        P=0.233        (e)      |
|INCIDENTAL TUMOR       |  P=0.007 **   P=0.324        (e)        P=0.074    |  P=0.788        (e)        P=0.233        (e)      |
|LOGISTIC REGRESSION    |  P=0.023 *    P=0.248        (e)        P=0.111    |  P=0.788        (e)        P=0.233        (e)      |
|COCHRAN-ARMITAGE       |  P=0.010 *                                         |  P=0.785N                                          |
|FISHER EXACT           |               P=0.248        (e)        P=0.029 *  |                 (e)        P=0.500        (e)      |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  20
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin                                                                                                                             |
|     Trichoepithelioma, Kertatocanthoma, Squamous Cell Carcinoma,                                                                |
|     Basal Cell Carcinoma or Adenoma, Papilloma Squamous, or Papilloma                                                           |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  3/60 (5%)    3/60 (5%)    3/59 (5%)    9/60 (15%) |  1/60 (2%)    0/59 (0%)    2/60 (3%)    0/60 (0%)  |
|ADJUSTED (b)           |   8.8%         8.7%        15.4%        38.4%      |   3.2%         0.0%        16.7%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    3/8 (38%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          2/9 (22%)  |                                                    |
|TERMINAL (d)           |  3/34 (9%)    2/32 (6%)    1/14 (7%)    0/0        |  1/31 (3%)    0/30 (0%)    1/8 (13%)    0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      689          596          450 (I)   |   736 (T)      ---          637          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.645      P=0.305      P=0.001 ** |  P=0.373      P=0.507N     P=0.156        (e)      |
|INCIDENTAL TUMOR       |  P=0.003 **   P=0.624N     P=0.452      P=0.067    |  P=0.630      P=0.507N     P=0.320        (e)      |
|LOGISTIC REGRESSION    |  P=0.002 **   P=0.647N     P=0.494      P=0.034 *  |  P=0.585      P=0.507N     P=0.289        (e)      |
|COCHRAN-ARMITAGE       |  P=0.014 *                                         |  P=0.454N                                          |
|FISHER EXACT           |               P=0.660N     P=0.652      P=0.063    |               P=0.504N     P=0.500      P=0.500N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin: Subcutaneous Tissue                                                                                                        |
|     Fibroma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  2/60 (3%)    2/60 (3%)    6/59 (10%)   1/60 (2%)  |  0/60 (0%)    1/59 (2%)    0/60 (0%)    0/60 (0%)  |
|ADJUSTED (b)           |   5.2%         5.7%        34.0%         9.1%      |   0.0%         2.2%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  1/34 (3%)    1/32 (3%)    4/14 (29%)   0/0        |  0/31 (0%)    0/30 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   614          692          524          520       |   ---          510          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.001 **   P=0.690      P=0.016 *    P=0.214    |  P=0.983N     P=0.496        (e)          (e)      |
|INCIDENTAL TUMOR       |  P=0.446      P=0.658N     P=0.051      P=0.983    |  P=0.310N     P=0.545        (e)          (e)      |
|LOGISTIC REGRESSION    |  P=0.189      P=0.682N     P=0.068      P=0.814    |  P=0.468N     P=0.491        (e)          (e)      |
|COCHRAN-ARMITAGE       |  P=0.373N                                          |  P=0.575N                                          |
|FISHER EXACT           |               P=0.691N     P=0.131      P=0.500N   |               P=0.496        (e)          (e)      |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  21
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin: Subcutaneous Tissue                                                                                                        |
|     Neurofibrosarcoma, Fibrosarcoma, Sarcoma,                                                                                   |
|     Neurofibroma, or Fibroma                                                                                                    |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  3/60 (5%)    3/60 (5%)    7/59 (12%)   1/60 (2%)  |  1/60 (2%)    1/59 (2%)    1/60 (2%)    0/60 (0%)  |
|ADJUSTED (b)           |   7.6%         8.0%        36.5%         9.1%      |   3.1%         2.2%         5.6%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  1/34 (3%)    1/32 (3%)    4/14 (29%)   0/0        |  0/31 (0%)    0/30 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   614          663          524          520       |   717          510          643          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.002 **   P=0.646N     P=0.024 *    P=0.214    |  P=0.863      P=0.758      P=0.541        (e)      |
|INCIDENTAL TUMOR       |  P=0.516      P=0.590N     P=0.093      P=0.983    |  P=0.292N     P=0.713N     P=0.642N       (e)      |
|LOGISTIC REGRESSION    |  P=0.254      P=0.652N     P=0.086      P=0.694N   |  P=0.513N     P=0.756      P=0.681        (e)      |
|COCHRAN-ARMITAGE       |  P=0.229N                                          |  P=0.321N                                          |
|FISHER EXACT           |               P=0.660N     P=0.154      P=0.309N   |               P=0.748      P=0.752N     P=0.500N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Spleen                                                                                                                           |
|     Fibroma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  2/60 (3%)    0/60 (0%)    0/59 (0%)    0/60 (0%)  |  0/60 (0%)    0/57 (0%)    0/60 (0%)    0/59 (0%)  |
|ADJUSTED (b)           |   5.9%         0.0%         0.0%         0.0%      |   0.0%         0.0%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  2/34 (6%)    0/32 (0%)    0/14 (0%)    0/0        |  0/31 (0%)    0/30 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      ---          ---          ---       |   ---          ---          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.797N     P=0.252N     P=0.448N       (e)      |    (e)          (e)          (e)          (e)      |
|INCIDENTAL TUMOR       |  P=0.797N     P=0.252N     P=0.448N       (e)      |    (e)          (e)          (e)          (e)      |
|LOGISTIC REGRESSION    |  P=0.797N     P=0.252N     P=0.448N       (e)      |    (e)          (e)          (e)          (e)      |
|COCHRAN-ARMITAGE       |  P=0.243N                                          |    (e)                                             |
|FISHER EXACT           |               P=0.248N     P=0.252N     P=0.248N   |                 (e)          (e)          (e)      |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  22
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Stomach, Forestomach                                                                                                             |
|     Squamous Cell Carcinoma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    9/60 (15%)  28/59 (47%)  14/60 (23%) |  0/60 (0%)    3/59 (5%)    9/60 (15%)   6/60 (10%) |
|ADJUSTED (b)           |   0.0%        24.3%        89.4%        57.6%      |   0.0%         9.4%        57.6%        48.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    1/10 (10%)   1/8 (13%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     2/8 (25%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          2/9 (22%)  |                                                    |
|TERMINAL (d)           |  0/34 (0%)    6/32 (19%)  11/14 (79%)   0/0        |  0/31 (0%)    2/30 (7%)    3/8 (38%)    0/0        |
|FIRST INCIDENCE (DAYS) |   ---          600          450 (I)      423       |   ---          713          628          325       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.003 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.121      P<0.001 **   P=0.001 ** |
|INCIDENTAL TUMOR       |  P<0.001 **   P=0.002 **   P<0.001 **   P=0.232    |  P<0.001 **   P=0.130      P<0.001 **   P=0.127    |
|LOGISTIC REGRESSION    |  P<0.001 **   P=0.003 **   P<0.001 **   P=0.001 ** |  P<0.001 **   P=0.124      P<0.001 **   P=0.046 *  |
|COCHRAN-ARMITAGE       |  P=0.012 *                                         |  P=0.058                                           |
|FISHER EXACT           |               P=0.001 **   P<0.001 **   P<0.001 ** |               P=0.119      P=0.001 **   P=0.014 *  |
|=================================================================================================================================|
|                                                                                                                                 |
|Stomach, Forestomach                                                                                                             |
|     Squamous Cell Carcinoma or Papilloma Squamous                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)   35/60 (58%)  46/59 (78%)  51/60 (85%) |  0/60 (0%)   17/59 (29%)  42/60 (70%)  27/60 (45%) |
|ADJUSTED (b)           |   0.0%        80.8%       100.0%       100.0%      |   0.0%        47.3%       100.0%       100.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    2/10 (20%)   4/10 (40%)   8/8 (100%) |  0/10 (0%)    1/10 (10%)   5/8 (63%)    8/8 (100%) |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          9/9 (100%) |                                                    |
|TERMINAL (d)           |  0/34 (0%)   24/32 (75%)  14/14 (100%)  0/0        |  0/31 (0%)   12/30 (40%)   8/8 (100%)   0/0        |
|FIRST INCIDENCE (DAYS) |   ---          450 (I)      404          368       |   ---          463 (I)      451          325       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|LOGISTIC REGRESSION    |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P<0.001 **   P<0.001 **   P<0.001 ** |               P<0.001 **   P<0.001 **   P<0.001 ** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  23
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Stomach, Forestomach                                                                                                             |
|     Squamous Cell Papilloma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)   31/60 (52%)  36/59 (61%)  46/60 (77%) |  0/60 (0%)   14/59 (24%)  37/60 (62%)  24/60 (40%) |
|ADJUSTED (b)           |   0.0%        74.8%        89.0%        97.7%      |   0.0%        39.6%       100.0%        95.3%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    2/10 (20%)   3/10 (30%)   8/8 (100%) |  0/10 (0%)    1/10 (10%)   5/8 (63%)    7/8 (88%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          8/9 (89%)  |                                                    |
|TERMINAL (d)           |  0/34 (0%)   22/32 (69%)  10/14 (71%)   0/0        |  0/31 (0%)   10/30 (33%)   8/8 (100%)   0/0        |
|FIRST INCIDENCE (DAYS) |   ---          450 (I)      404          368       |   ---          463 (I)      451          325       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|LOGISTIC REGRESSION    |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |  P<0.001 **   P<0.001 **   P<0.001 **   P<0.001 ** |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P<0.001 **   P<0.001 **   P<0.001 ** |               P<0.001 **   P<0.001 **   P<0.001 ** |
|=================================================================================================================================|
|                                                                                                                                 |
|Testes                                                                                                                           |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 55/60 (92%)  54/60 (90%)  55/59 (93%)  52/60 (87%) |                                                    |
|ADJUSTED (b)           | 100.0%       100.0%       100.0%       100.0%      |                                                    |
|INTERIM SACRIFICE 1 (c)|  8/10 (80%)   6/10 (60%)  10/10 (100%)  8/8 (100%) |                                                    |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          9/9 (100%) |                                                    |
|TERMINAL (d)           | 34/34 (100%) 32/32 (100%) 14/14 (100%)  0/0        |                                                    |
|FIRST INCIDENCE (DAYS) |   450 (I)      450 (I)      404          361       |                                                    |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.566N     P<0.001 **   P<0.001 ** |                                                    |
|INCIDENTAL TUMOR       |  P=0.018 *    P=0.240N     P=0.037 *    P=0.087    |                                                    |
|LOGISTIC REGRESSION    |  P=0.016 *    P=0.339N     P=0.151      P=0.065    |                                                    |
|COCHRAN-ARMITAGE       |  P=0.223N                                          |                                                    |
|FISHER EXACT           |               P=0.500N     P=0.511      P=0.279N   |                                                    |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  24
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Thyroid Gland: C-Cell                                                                                                            |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  4/60 (7%)   15/59 (25%)   4/59 (7%)    6/60 (10%) |  4/60 (7%)    5/57 (9%)    4/60 (7%)    0/60 (0%)  |
|ADJUSTED (b)           |  10.9%        41.6%        22.5%        35.7%      |  11.6%        12.9%        14.8%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    1/8 (13%)  |  0/10 (0%)    1/10 (10%)   0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          2/9 (22%)  |                                                    |
|TERMINAL (d)           |  3/34 (9%)   11/31 (35%)   2/14 (14%)   0/0        |  3/31 (10%)   1/30 (3%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   614          621          685          452 (I)   |   526          465 (I)      513          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.005 **   P=0.253      P=0.012 *  |  P=0.542      P=0.492      P=0.243        (e)      |
|INCIDENTAL TUMOR       |  P=0.163      P=0.006 **   P=0.478      P=0.373    |  P=0.097N     P=0.460      P=0.509N     P=0.140N   |
|LOGISTIC REGRESSION    |  P=0.043 *    P=0.006 **   P=0.443      P=0.120    |  P=0.357N     P=0.472      P=0.569      P=0.676N   |
|COCHRAN-ARMITAGE       |  P=0.261N                                          |  P=0.032N*                                         |
|FISHER EXACT           |               P=0.005 **   P=0.632      P=0.372    |               P=0.467      P=0.641N     P=0.059N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Thyroid Gland: C-Cell                                                                                                            |
|     Carcinoma                                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    1/59 (2%)    2/59 (3%)    0/60 (0%)  |  0/60 (0%)    1/57 (2%)    0/60 (0%)    0/60 (0%)  |
|ADJUSTED (b)           |   0.0%         2.7%        11.8%         0.0%      |   0.0%         3.3%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  0/34 (0%)    0/31 (0%)    1/14 (7%)    0/0        |  0/31 (0%)    1/30 (3%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   ---          692          686          ---       |   ---          736 (T)      ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.297      P=0.511      P=0.101        (e)      |  P=0.999      P=0.493        (e)          (e)      |
|INCIDENTAL TUMOR       |  P=0.621      P=0.674      P=0.241        (e)      |  P=0.999      P=0.493        (e)          (e)      |
|LOGISTIC REGRESSION    |  P=0.530      P=0.504      P=0.156        (e)      |  P=0.999      P=0.493        (e)          (e)      |
|COCHRAN-ARMITAGE       |  P=0.513N                                          |  P=0.572N                                          |
|FISHER EXACT           |               P=0.496      P=0.244        (e)      |               P=0.487        (e)          (e)      |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  25
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Thyroid Gland: C-Cell                                                                                                            |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  4/60 (7%)   16/59 (27%)   6/59 (10%)   6/60 (10%) |  4/60 (7%)    6/57 (11%)   4/60 (7%)    0/60 (0%)  |
|ADJUSTED (b)           |  10.9%        43.1%        32.5%        35.7%      |  11.6%        15.9%        14.8%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    1/8 (13%)  |  0/10 (0%)    1/10 (10%)   0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          2/9 (22%)  |                                                    |
|TERMINAL (d)           |  3/34 (9%)   11/31 (35%)   3/14 (21%)   0/0        |  3/31 (10%)   2/30 (7%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   614          621          685          452 (I)   |   526          465 (I)      513          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.003 **   P=0.062      P=0.012 *  |  P=0.529      P=0.365      P=0.243        (e)      |
|INCIDENTAL TUMOR       |  P=0.103      P=0.005 **   P=0.229      P=0.373    |  P=0.103N     P=0.335      P=0.509N     P=0.140N   |
|LOGISTIC REGRESSION    |  P=0.026 *    P=0.003 **   P=0.171      P=0.120    |  P=0.183N     P=0.345      P=0.569      P=0.676N   |
|COCHRAN-ARMITAGE       |  P=0.222N                                          |  P=0.024N*                                         |
|FISHER EXACT           |               P=0.003 **   P=0.361      P=0.372    |               P=0.339      P=0.641N     P=0.059N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Thyroid Gland: Follicular Cell                                                                                                   |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/60 (2%)    1/59 (2%)    2/59 (3%)    2/60 (3%)  |  0/60 (0%)    0/57 (0%)    3/60 (5%)    2/60 (3%)  |
|ADJUSTED (b)           |   2.9%         3.2%         8.5%         5.6%      |   0.0%         0.0%        20.1%         8.1%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  1/34 (3%)    1/31 (3%)    0/14 (0%)    0/0        |  0/31 (0%)    0/30 (0%)    1/8 (13%)    0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      729 (T)      678          425       |   ---          ---          538          310       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.007 **   P=0.741      P=0.308      P=0.160    |  P<0.001 **     (e)        P=0.026 *    P=0.099    |
|INCIDENTAL TUMOR       |  P=0.370      P=0.741      P=0.677      P=0.913    |  P=0.377        (e)        P=0.093      P=0.860    |
|LOGISTIC REGRESSION    |  P=0.196      P=0.741      P=0.394      P=0.561    |  P=0.151        (e)        P=0.078      P=0.609    |
|COCHRAN-ARMITAGE       |  P=0.385                                           |  P=0.160                                           |
|FISHER EXACT           |               P=0.748      P=0.494      P=0.500    |                 (e)        P=0.122      P=0.248    |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  26
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Thyroid Gland: Follicular Cell                                                                                                   |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/60 (2%)    1/59 (2%)    3/59 (5%)    2/60 (3%)  |  0/60 (0%)    1/57 (2%)    3/60 (5%)    2/60 (3%)  |
|ADJUSTED (b)           |   2.9%         3.2%        15.1%         5.6%      |   0.0%         3.3%        20.1%         8.1%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  1/34 (3%)    1/31 (3%)    1/14 (7%)    0/0        |  0/31 (0%)    1/30 (3%)    1/8 (13%)    0/0        |
|FIRST INCIDENCE (DAYS) |   729 (T)      729 (T)      678          425       |   ---          736 (T)      538          310       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.002 **   P=0.741      P=0.116      P=0.160    |  P<0.001 **   P=0.493      P=0.026 *    P=0.099    |
|INCIDENTAL TUMOR       |  P=0.218      P=0.741      P=0.333      P=0.913    |  P=0.367      P=0.493      P=0.093      P=0.860    |
|LOGISTIC REGRESSION    |  P=0.120      P=0.741      P=0.185      P=0.561    |  P=0.137      P=0.493      P=0.078      P=0.609    |
|COCHRAN-ARMITAGE       |  P=0.403                                           |  P=0.264                                           |
|FISHER EXACT           |               P=0.748      P=0.303      P=0.500    |               P=0.487      P=0.122      P=0.248    |
|=================================================================================================================================|
|                                                                                                                                 |
|Tongue                                                                                                                           |
|     Squamous Cell Carcinoma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/60 (0%)    0/59 (0%)   19/60 (32%) |  0/60 (0%)    0/59 (0%)   13/60 (22%)   8/60 (13%) |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%        57.5%      |   0.0%         0.0%        57.0%        34.7%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     1/8 (13%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          1/9 (11%)  |                                                    |
|TERMINAL (d)           |  0/34 (0%)    0/32 (0%)    0/14 (0%)    0/0        |  0/31 (0%)    0/30 (0%)    3/8 (38%)    0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          327       |   ---          ---          513          294       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **     (e)          (e)        P<0.001 ** |  P<0.001 **     (e)        P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P=0.005 **     (e)          (e)        P=0.245    |  P=0.058        (e)        P<0.001 **   P=0.273    |
|LOGISTIC REGRESSION    |  P<0.001 **     (e)          (e)        P=0.004 ** |  P=0.011 *      (e)        P<0.001 **   P=0.100    |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P=0.005 **                                        |
|FISHER EXACT           |                 (e)          (e)        P<0.001 ** |                 (e)        P<0.001 **   P=0.003 ** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  27
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Tongue                                                                                                                           |
|     Squamous Cell Carcinoma or Papilloma Squamous                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    2/60 (3%)    9/59 (15%)  40/60 (67%) |  0/60 (0%)    3/59 (5%)   18/60 (30%)  26/60 (43%) |
|ADJUSTED (b)           |   0.0%         5.9%        36.7%        83.4%      |   0.0%         9.1%        77.1%        78.3%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    1/10 (10%)   3/8 (38%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     3/8 (38%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          3/9 (33%)  |                                                    |
|TERMINAL (d)           |  0/34 (0%)    1/32 (3%)    3/14 (21%)   0/0        |  0/31 (0%)    2/30 (7%)    5/8 (63%)    0/0        |
|FIRST INCIDENCE (DAYS) |   ---          694          452 (I)      327       |   ---          677          479          233       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.236      P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.124      P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P<0.001 **   P=0.324      P=0.005 **   P=0.002 ** |  P<0.001 **   P=0.130      P<0.001 **   P=0.005 ** |
|LOGISTIC REGRESSION    |  P<0.001 **   P=0.248      P=0.001 **   P<0.001 ** |  P<0.001 **   P=0.123      P<0.001 **   P<0.001 ** |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P=0.248      P=0.001 **   P<0.001 ** |               P=0.119      P<0.001 **   P<0.001 ** |
|=================================================================================================================================|
|                                                                                                                                 |
|Tongue                                                                                                                           |
|     Squamous Cell Papilloma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    2/60 (3%)    9/59 (15%)  21/60 (35%) |  0/60 (0%)    3/59 (5%)    5/60 (8%)   18/60 (30%) |
|ADJUSTED (b)           |   0.0%         5.9%        36.7%        59.3%      |   0.0%         9.1%        33.4%        65.2%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    1/10 (10%)   3/8 (38%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     2/8 (25%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          2/9 (22%)  |                                                    |
|TERMINAL (d)           |  0/34 (0%)    1/32 (3%)    3/14 (21%)   0/0        |  0/31 (0%)    2/30 (7%)    2/8 (25%)    0/0        |
|FIRST INCIDENCE (DAYS) |   ---          694          452 (I)      337       |   ---          677          479          233       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.236      P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.124      P=0.002 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P<0.001 **   P=0.324      P=0.005 **   P=0.030 *  |  P<0.001 **   P=0.130      P=0.017 *    P=0.027 *  |
|LOGISTIC REGRESSION    |  P<0.001 **   P=0.248      P=0.001 **   P<0.001 ** |  P<0.001 **   P=0.123      P=0.017 *    P<0.001 ** |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P=0.248      P=0.001 **   P<0.001 ** |               P=0.119      P=0.029 *    P<0.001 ** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  28
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Uterus                                                                                                                           |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |  0/60 (0%)    1/59 (2%)    2/60 (3%)    0/60 (0%)  |
|ADJUSTED (b)           |                                                    |   0.0%         3.2%        25.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|                                                    |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |                                                    |
|TERMINAL (d)           |                                                    |  0/31 (0%)    0/30 (0%)    2/8 (25%)    0/0        |
|FIRST INCIDENCE (DAYS) |                                                    |   ---          729          736 (T)      ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |  P=0.144      P=0.500      P=0.027 *      (e)      |
|INCIDENTAL TUMOR       |                                                    |  P=0.259      P=0.540      P=0.027 *      (e)      |
|LOGISTIC REGRESSION    |                                                    |  P=0.186      P=0.506      P=0.027 *      (e)      |
|COCHRAN-ARMITAGE       |                                                    |  P=0.513N                                          |
|FISHER EXACT           |                                                    |               P=0.496      P=0.248        (e)      |
|=================================================================================================================================|
|                                                                                                                                 |
|Uterus                                                                                                                           |
|     Polyp Stromal                                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |  9/60 (15%)   5/59 (8%)    7/60 (12%)   1/60 (2%)  |
|ADJUSTED (b)           |                                                    |  26.4%        13.7%        36.0%        11.1%      |
|INTERIM SACRIFICE 1 (c)|                                                    |  0/10 (0%)    1/10 (10%)   0/8 (0%)     1/8 (13%)  |
|INTERIM SACRIFICE 2 (c)|                                                    |                                                    |
|TERMINAL (d)           |                                                    |  7/31 (23%)   3/30 (10%)   2/8 (25%)    0/0        |
|FIRST INCIDENCE (DAYS) |                                                    |   547          463 (I)      450          463 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |  P=0.126      P=0.207N     P=0.143      P=0.455    |
|INCIDENTAL TUMOR       |                                                    |  P=0.611      P=0.220N     P=0.612      P=0.455    |
|LOGISTIC REGRESSION    |                                                    |  P=0.435N     P=0.192N     P=0.581      P=0.751    |
|COCHRAN-ARMITAGE       |                                                    |  P=0.016N*                                         |
|FISHER EXACT           |                                                    |               P=0.207N     P=0.395N     P=0.008N** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  29
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Uterus                                                                                                                           |
|     Sarcoma Stromal                                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |  3/60 (5%)    0/59 (0%)    0/60 (0%)    0/60 (0%)  |
|ADJUSTED (b)           |                                                    |   6.8%         0.0%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|                                                    |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |                                                    |
|TERMINAL (d)           |                                                    |  0/31 (0%)    0/30 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |                                                    |   424          ---          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |  P=0.407N     P=0.116N     P=0.233N     P=0.786N   |
|INCIDENTAL TUMOR       |                                                    |  P=0.094N     P=0.086N     P=0.053N     P=0.140N   |
|LOGISTIC REGRESSION    |                                                    |  P=0.073N     P=0.127N     P=0.087N     P=0.143N   |
|COCHRAN-ARMITAGE       |                                                    |  P=0.134N                                          |
|FISHER EXACT           |                                                    |               P=0.125N     P=0.122N     P=0.122N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Uterus                                                                                                                           |
|     Sarcoma Stromal or Polyp Stromal                                                                                            |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    | 12/60 (20%)   5/59 (8%)    7/60 (12%)   1/60 (2%)  |
|ADJUSTED (b)           |                                                    |  31.4%        13.7%        36.0%        11.1%      |
|INTERIM SACRIFICE 1 (c)|                                                    |  0/10 (0%)    1/10 (10%)   0/8 (0%)     1/8 (13%)  |
|INTERIM SACRIFICE 2 (c)|                                                    |                                                    |
|TERMINAL (d)           |                                                    |  7/31 (23%)   3/30 (10%)   2/8 (25%)    0/0        |
|FIRST INCIDENCE (DAYS) |                                                    |   424          463 (I)      450          463 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |  P=0.289      P=0.065N     P=0.355      P=0.584    |
|INCIDENTAL TUMOR       |                                                    |  P=0.303N     P=0.058N     P=0.236N     P=0.623N   |
|LOGISTIC REGRESSION    |                                                    |  P=0.134N     P=0.059N     P=0.293N     P=0.344N   |
|COCHRAN-ARMITAGE       |                                                    |  P=0.005N**                                        |
|FISHER EXACT           |                                                    |               P=0.061N     P=0.159N     P=0.001N** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  30
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|Zymbal's Gland                                                                                                                   |
|     Carcinoma                                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/60 (0%)    0/59 (0%)    3/60 (5%)  |  0/60 (0%)    1/59 (2%)    0/60 (0%)    4/60 (7%)  |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%         6.0%      |   0.0%         2.9%         0.0%        36.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     1/8 (13%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  0/34 (0%)    0/32 (0%)    0/14 (0%)    0/0        |  0/31 (0%)    0/30 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          395       |   ---          713          ---          336       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.005 **     (e)          (e)        P=0.093    |  P<0.001 **   P=0.506        (e)        P=0.003 ** |
|INCIDENTAL TUMOR       |  P=0.376        (e)          (e)        P=0.840    |  P=0.063      P=0.540        (e)        P=0.319    |
|LOGISTIC REGRESSION    |  P=0.058        (e)          (e)        P=0.441    |  P=0.028 *    P=0.503        (e)        P=0.103    |
|COCHRAN-ARMITAGE       |  P=0.009 **                                        |  P=0.011 *                                         |
|FISHER EXACT           |                 (e)          (e)        P=0.122    |               P=0.496        (e)        P=0.059    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangioma                                                                                                                  |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/60 (0%)    0/59 (0%)    2/60 (3%)  |  0/60 (0%)    1/59 (2%)    0/60 (0%)    1/60 (2%)  |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%         9.0%      |   0.0%         3.3%         0.0%         3.1%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  0/34 (0%)    0/32 (0%)    0/14 (0%)    0/0        |  0/31 (0%)    1/30 (3%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          485       |   ---          736 (T)      ---          371       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.005 **     (e)          (e)        P=0.090    |  P=0.120      P=0.493        (e)        P=0.375    |
|INCIDENTAL TUMOR       |  P=0.500        (e)          (e)        P=0.913    |  P=0.447      P=0.493        (e)        P=0.860    |
|LOGISTIC REGRESSION    |  P=0.111        (e)          (e)        P=0.394    |  P=0.481      P=0.493        (e)        P=0.842    |
|COCHRAN-ARMITAGE       |  P=0.042 *                                         |  P=0.466                                           |
|FISHER EXACT           |                 (e)          (e)        P=0.248    |               P=0.496        (e)        P=0.500    |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  31
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangiosarcoma or Hemangioma                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/60 (0%)    0/60 (0%)    0/59 (0%)    2/60 (3%)  |  0/60 (0%)    1/59 (2%)    1/60 (2%)    1/60 (2%)  |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%         9.0%      |   0.0%         3.3%         8.3%         3.1%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  0/34 (0%)    0/32 (0%)    0/14 (0%)    0/0        |  0/31 (0%)    1/30 (3%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          485       |   ---          736 (T)      709          371       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.005 **     (e)          (e)        P=0.090    |  P=0.038 *    P=0.493      P=0.299      P=0.375    |
|INCIDENTAL TUMOR       |  P=0.500        (e)          (e)        P=0.913    |  P=0.284      P=0.493      P=0.500      P=0.860    |
|LOGISTIC REGRESSION    |  P=0.111        (e)          (e)        P=0.394    |  P=0.268      P=0.493      P=0.362      P=0.842    |
|COCHRAN-ARMITAGE       |  P=0.042 *                                         |  P=0.487                                           |
|FISHER EXACT           |                 (e)          (e)        P=0.248    |               P=0.496      P=0.500      P=0.500    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Leukemia: Lymphocytic, Monocytic, Mononuclear,                                                                              |
|     or Undifferentiated                                                                                                         |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 16/60 (27%)  11/60 (18%)   9/59 (15%)   6/60 (10%) | 13/60 (22%)  17/59 (29%)  14/60 (23%)   0/60 (0%)  |
|ADJUSTED (b)           |  42.6%        30.5%        42.0%        34.6%      |  34.9%        44.1%        69.3%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          1/9 (11%)  |                                                    |
|TERMINAL (d)           | 13/34 (38%)   8/32 (25%)   4/14 (29%)   0/0        |  8/31 (26%)  10/30 (33%)   4/8 (50%)    0/0        |
|FIRST INCIDENCE (DAYS) |   605          591          590          459       |   597          434          405          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.216N     P=0.459      P=0.001 ** |  P=0.025 *    P=0.265      P=0.005 **     (e)      |
|INCIDENTAL TUMOR       |  P=0.410      P=0.171N     P=0.375N     P=0.719    |  P=0.104N     P=0.233      P=0.330        (e)      |
|LOGISTIC REGRESSION    |  P=0.152      P=0.141N     P=0.311N     P=0.219    |  P=0.323N     P=0.262      P=0.164      P=0.372N   |
|COCHRAN-ARMITAGE       |  P=0.022N*                                         |  P<0.001N**                                        |
|FISHER EXACT           |               P=0.191N     P=0.096N     P=0.016N*  |               P=0.246      P=0.500      P<0.001N** |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  32
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Mesothelioma Benign, Malignant, NOS                                                                                         |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  3/60 (5%)    4/60 (7%)    4/59 (7%)    2/60 (3%)  |  0/60 (0%)    0/59 (0%)    0/60 (0%)    0/60 (0%)  |
|ADJUSTED (b)           |   7.8%        10.4%        18.1%        10.6%      |   0.0%         0.0%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    1/10 (10%)   0/8 (0%)   |  0/10 (0%)    0/10 (0%)    0/8 (0%)     0/8 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          0/9 (0%)   |                                                    |
|TERMINAL (d)           |  2/34 (6%)    1/32 (3%)    2/14 (14%)   0/0        |  0/31 (0%)    0/30 (0%)    0/8 (0%)     0/0        |
|FIRST INCIDENCE (DAYS) |   485          621          450 (I)      493       |   ---          ---          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.034 *    P=0.505      P=0.228      P=0.217    |    (e)          (e)          (e)          (e)      |
|INCIDENTAL TUMOR       |  P=0.546N     P=0.430      P=0.315      P=0.160N   |    (e)          (e)          (e)          (e)      |
|LOGISTIC REGRESSION    |  P=0.606N     P=0.509      P=0.469      P=0.732N   |    (e)          (e)          (e)          (e)      |
|COCHRAN-ARMITAGE       |  P=0.332N                                          |    (e)                                             |
|FISHER EXACT           |               P=0.500      P=0.491      P=0.500N   |                 (e)          (e)          (e)      |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Benign Tumors                                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 57/60 (95%)  58/60 (97%)  56/59 (95%)  57/60 (95%) | 42/60 (70%)  48/59 (81%)  51/60 (85%)  40/60 (67%) |
|ADJUSTED (b)           | 100.0%       100.0%       100.0%       100.0%      |  93.2%       100.0%       100.0%       100.0%      |
|INTERIM SACRIFICE 1 (c)|  8/10 (80%)   8/10 (80%)  10/10 (100%)  8/8 (100%) |  1/10 (10%)   4/10 (40%)   5/8 (63%)    8/8 (100%) |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          9/9 (100%) |                                                    |
|TERMINAL (d)           | 34/34 (100%) 32/32 (100%) 14/14 (100%)  0/0        | 28/31 (90%)  30/30 (100%)  8/8 (100%)   0/0        |
|FIRST INCIDENCE (DAYS) |   340          450 (I)      404          337       |   463 (I)      463 (I)      405          233       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.445      P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.164      P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P=0.036 *    P=0.707      P=0.304      P=0.130    |  P<0.001 **   P=0.071      P<0.001 **   P<0.001 ** |
|LOGISTIC REGRESSION    |  P=0.026 *    P=0.656      P=0.268      P=0.072    |  P<0.001 **   P=0.073      P<0.001 **   P<0.001 ** |
|COCHRAN-ARMITAGE       |  P=0.524N                                          |  P=0.129N                                          |
|FISHER EXACT           |               P=0.500      P=0.652N     P=0.660N   |               P=0.109      P=0.040 *    P=0.422N   |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  33
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |   0 MG/KG      3 MG/KG      10 MG/KG     30 MG/KG  |
|                       |                                                    |                                                    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Tumors                                                                                                            |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 22/60 (37%)  28/60 (47%)  40/59 (68%)  47/60 (78%) | 20/60 (33%)  25/59 (42%)  46/60 (77%)  48/60 (80%) |
|ADJUSTED (b)           |  54.1%        60.5%        97.3%        93.6%      |  47.2%        62.9%       100.0%        94.9%      |
|INTERIM SACRIFICE 1 (c)|  0/10 (0%)    0/10 (0%)    2/10 (20%)   2/8 (25%)  |  0/10 (0%)    0/10 (0%)    0/8 (0%)     5/8 (63%)  |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          6/9 (67%)  |                                                    |
|TERMINAL (d)           | 16/34 (47%)  14/32 (44%)  13/14 (93%)   0/0        | 10/31 (32%)  16/30 (53%)   8/8 (100%)   0/0        |
|FIRST INCIDENCE (DAYS) |   485          591          404          327       |   424          434          255          184       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.199      P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.236      P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P<0.001 **   P=0.270      P<0.001 **   P=0.026 *  |  P<0.001 **   P=0.224      P<0.001 **   P=0.001 ** |
|LOGISTIC REGRESSION    |  P<0.001 **   P=0.247      P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.216      P<0.001 **   P<0.001 ** |
|COCHRAN-ARMITAGE       |  P<0.001 **                                        |  P<0.001 **                                        |
|FISHER EXACT           |               P=0.177      P<0.001 **   P<0.001 ** |               P=0.204      P<0.001 **   P<0.001 ** |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant and Benign Tumors                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 58/60 (97%)  58/60 (97%)  57/59 (97%)  60/60 (100%)| 49/60 (82%)  50/59 (85%)  56/60 (93%)  56/60 (93%) |
|ADJUSTED (b)           | 100.0%       100.0%       100.0%       100.0%      |  98.0%       100.0%       100.0%       100.0%      |
|INTERIM SACRIFICE 1 (c)|  8/10 (80%)   8/10 (80%)  10/10 (100%)  8/8 (100%) |  1/10 (10%)   4/10 (40%)   5/8 (63%)    8/8 (100%) |
|INTERIM SACRIFICE 2 (c)|  0/0          0/0          0/0          9/9 (100%) |                                                    |
|TERMINAL (d)           | 34/34 (100%) 32/32 (100%) 14/14 (100%)  0/0        | 30/31 (97%)  30/30 (100%)  8/8 (100%)   0/0        |
|FIRST INCIDENCE (DAYS) |   340          450 (I)      404          327       |   424          434          255          184       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P<0.001 **   P=0.512      P<0.001 **   P<0.001 ** |  P<0.001 **   P=0.455      P<0.001 **   P<0.001 ** |
|INCIDENTAL TUMOR       |  P=0.066      P=0.707      P=0.579      P=0.284    |  P<0.001 **   P=0.428      P=0.019 *    P<0.001 ** |
|LOGISTIC REGRESSION    |  P=0.005 **   P=0.566N     P=0.264      P=0.036 *  |  P<0.001 **   P=0.436      P=0.001 **   P<0.001 ** |
|COCHRAN-ARMITAGE       |  P=0.157                                           |  P=0.036 *                                         |
|FISHER EXACT           |               P=0.691N     P=0.684N     P=0.248    |               P=0.420      P=0.048 *    P=0.048 *  |
|=================================================================================================================================|
 
         DATE: 09/14/94                     EXPERIMENT: 05111 TEST: 01                                                 PAGE  34
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN RATS(FISCHER344) -- 1,2,3-TRICHLOROPROPANE
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
 
 (a)  Number of tumor-bearing animals / number of animals examined at site.
 (b)  Kaplan-Meier estimated lifetime tumor incidence after adjustment for 
      intercurrent mortality.
 (c)  Observed incidence at interim kill.
          Male interim sacrifices(days):   450  534
          Female interim sacrifices(days): 463
 (d)  Observed incidence at terminal kill.
 (f)  Beneath the control incidence are the P-values associated with the trend 
      test.  Beneath the dosed group incidence are the P-values corresponding to 
      pairwise comparisons between the controls and that dosed group.  The life
      table analysis regards tumors in animals dying  prior to terminal kill as
      being (directly or indirectly) the cause of death.  The incidental tumor 
      test regards these lesions as non-fatal.  Logistic regression is an alternative
      method for analyzing the incidence of non-fatal tumors.  The Cochran-Armitage
      and Fisher's exact tests compare directly the overall incidence rates.  
      For all tests, a negative trend is indicated by "N".
 (e)  Value of Statistic cannot be computed. 
 (I)  Interim sacrifice
 (T)  Terminal sacrifice
  #   Tumor rates based on number of animals necropsied.
 
  *   To the right of any statistical result, indicates significance at (P<=0.05).
  **  To the right of any statistical result, indicates significance at (P<=0.01).

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