National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/3366

TDMS Study 05114-02 Pathology Tables

NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: Battelle Northwest                             HEXACHLOROCYCLOPENTADIENE (HCCPD)                          DATE: 09/15/94       
EXPERIMENT: 05114 TEST: 02                                                                                     TIME: 07:14:57       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                   PAGE:       1        
CONT: N01ES2800301          ROUTE: RESPIRATORY EXPOSURE WHOLE BODY                                             NTP C#: 55607B       
PATHOLOGIST: E. GIDDENS                      HAILEY, JAMESR.                                                   CAS: 77-47-4         
------------------------------------------------------------------------------------------------------------------------------------
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
               REASONS FOR REMOVAL:    ALL                                                                                          
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
               REMOVAL DATE RANGE:     ALL                                                                                          
                                                                                                                                    
               TREATMENT GROUPS:       INCLUDE ALL                                                                                  
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08    
LAB: Battelle Northwest                             HEXACHLOROCYCLOPENTADIENE (HCCPD)                          DATE: 09/15/94      
EXPERIMENT: 05114 TEST: 02                                                                                     TIME: 07:14:57      
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                       
CONT: N01ES2800301          ROUTE: RESPIRATORY EXPOSURE WHOLE BODY                                             NTP C#: 55607B      
PATHOLOGIST: E. GIDDENS                      HAILEY, JAMESR.                                                   CAS: 77-47-4        
 
 
 
 
 
                                                    MICE(B6C3F1)
                                              ----------------------------
             FOR ALL DOSES THE TUMOR RATES IN THE FOLLOWING TISSUES/ORGANS ARE BASED ON NUMBER OF TISSUES EXAMINED.
             IN OTHER TISSUES/ORGANS, RATES ARE BASED ON NUMBER OF ANIMALS NECROPSIED.
 
 
                                                    ----------------
 
                                                    Adrenal Cortex
                                                    Adrenal Medulla
                                                    Bone Marrow
                                                    Brain
                                                    Clitoral Gland
                                                    Epididymis
                                                    Gallbladder
                                                    Heart
                                                    Islets, Pancreatic
                                                    Kidney
                                                    Larynx
                                                    Liver
                                                    Lung
                                                    Nose
                                                    Ovary
                                                    Pancreas
                                                    Parathyroid Gland
                                                    Pituitary Gland
                                                    Preputial Gland
                                                    Prostate
                                                    Salivary Glands
                                                    Spleen
                                                    Testes
                                                    Thymus
                                                    Thyroid Gland
                                                    Urinary Bladder
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: Battelle Northwest                             HEXACHLOROCYCLOPENTADIENE (HCCPD)                          DATE: 09/15/94       
EXPERIMENT: 05114 TEST: 02                                                                                     TIME: 07:14:57       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                        
CONT: N01ES2800301          ROUTE: RESPIRATORY EXPOSURE WHOLE BODY                                             NTP C#: 55607B       
PATHOLOGIST: E. GIDDENS                      HAILEY, JAMESR.                                                   CAS: 77-47-4         
 
 
 
 
 
                                     SUMMARY OF STATISTICALLY SIGNIFICANT (P<=.05) RESULTS
                               IN THE ANALYSIS OF THE STUDY OF HEXACHLOROCYCLOPENTADIENE (HCCPD)
 
              ====================================================================================================
 
                                                           MALE MICE
                                                          -------------
                                 ORGAN                               MORPHOLOGY
                                -------                             ------------
 
                             Harderian Gland               Adenoma
                             Liver                         Hepatocellular Adenoma
                                                           Hepatocellular Carcinoma
                                                           Hepatocellular Carcinoma or Hepatocellular Adenoma
                                                           Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma
                             Lung                          Alveolar/Bronchiolar Carcinoma
                             Thyroid Gland: Follicular Cell
                                                           Adenoma
                             All Organs                    Malignant Lymphoma And Histiocytic Sarcoma
                                                           Malignant Lymphoma: Histiocytic, Lymphocytic, Mixed,
                                                            NOS, or Undifferentiated Cell Type
                                                           Benign Tumors
                                                           Malignant Tumors
                                                           Malignant and Benign Tumors
 
              ====================================================================================================
 
                                                           FEMALE MICE
                                                          -------------
                                 ORGAN                               MORPHOLOGY
                                -------                             ------------
 
                             Stomach, Forestomach          Squamous Cell Papilloma
                             All Organs                    Histiocytic Sarcoma
                                                           Malignant Lymphoma And Histiocytic Sarcoma
                                                           Malignant Lymphoma: Histiocytic, Lymphocytic, Mixed,
                                                            NOS, or Undifferentiated Cell Type
                                                           Benign Tumors
                                                           Malignant Tumors
                                                           Malignant and Benign Tumors
 
              ====================================================================================================
         DATE: 09/15/94                     EXPERIMENT: 05114 TEST: 02                                                 PAGE   1
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- HEXACHLOROCYCLOPENTADIENE (HCCPD)
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   | CONTROL    0.01 PPM   0.05 PPM   0.2  PPM   0.5  PP|   CONTROL      0.01 PPM     0.05 PPM     0.2   PP  |
|                       |                                  SE65       SE42   |                                          M         |
|=================================================================================================================================|
|                                                                                                                                 |
|Harderian Gland                                                                                                                  |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |  #          #          #          #          #     |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)             7/90 (8%)  3/60 (5%)  7/60 (12%  9/190 (5%  5/160 (3%  4/60 (7%)    6/60 (10%)   4/60 (7%)    2/60 (3%)  |
|ADJUSTED (b)            19.0%       9.1%      16.7%       8.4%       6.8%   |  12.9%        18.8%        12.1%         6.4%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  0/10 (0%)  0/10 (0%)  0/10 (0%)  0/20 (0%)  0/20 (0%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    1/10 (10%) |
|TERMINAL (d)            6/35 (17%  3/33 (9%)  7/42 (17%  6/102 (6%  5/74 (7%)  4/31 (13%)   6/32 (19%)   2/30 (7%)    1/21 (5%)  |
|FIRST INCIDENCE (DAYS) | 627        729 (T)    729 (T)    654        729 (T)|   736 (T)      736 (T)      660          458 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P=0.051N   P=0.183N   P=0.478N   P=0.076N   P=0.046N*  P=0.324N     P=0.387      P=0.631      P=0.493N   |
|INCIDENTAL TUMOR       |P=0.064N   P=0.190N   P=0.492N   P=0.085N   P=0.061N   P=0.298N     P=0.387      P=0.623N     P=0.493N   |
|LOGISTIC REGRESSION    |P=0.061N   P=0.169N   P=0.522N   P=0.077N   P=0.056N   P=0.246N     P=0.387      P=0.635N     P=0.399N   |
|COCHRAN-ARMITAGE       |P=0.036N*                                           |  P=0.162N                                          |
|FISHER EXACT           |           P=0.377N   P=0.300    P=0.224N   P=0.092N                P=0.372      P=0.641N     P=0.340N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Harderian Gland                                                                                                                  |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |  #          #          #          #          #     |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)             7/90 (8%)  3/60 (5%)  7/60 (12%  9/190 (5%  6/160 (4%  5/60 (8%)    6/60 (10%)   4/60 (7%)    2/60 (3%)  |
|ADJUSTED (b)            19.0%       9.1%      16.7%       8.4%       8.1%   |  15.5%        18.8%        12.1%         6.4%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  0/10 (0%)  0/10 (0%)  0/10 (0%)  0/20 (0%)  0/20 (0%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    1/10 (10%) |
|TERMINAL (d)            6/35 (17%  3/33 (9%)  7/42 (17%  6/102 (6%  6/74 (8%)  4/31 (13%)   6/32 (19%)   2/30 (7%)    1/21 (5%)  |
|FIRST INCIDENCE (DAYS) | 627        729 (T)    729 (T)    654        729 (T)|   693          736 (T)      660          458 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P=0.093N   P=0.183N   P=0.478N   P=0.076N   P=0.076N   P=0.262N     P=0.520      P=0.510N     P=0.358N   |
|INCIDENTAL TUMOR       |P=0.112N   P=0.190N   P=0.492N   P=0.085N   P=0.097N   P=0.230N     P=0.529      P=0.461N     P=0.332N   |
|LOGISTIC REGRESSION    |P=0.109N   P=0.169N   P=0.522N   P=0.077N   P=0.093N   P=0.192N     P=0.521      P=0.487N     P=0.272N   |
|COCHRAN-ARMITAGE       |P=0.066N                                            |  P=0.123N                                          |
|FISHER EXACT           |           P=0.377N   P=0.300    P=0.224N   P=0.141N                P=0.500      P=0.500N     P=0.219N   |
|=================================================================================================================================|
 
         DATE: 09/15/94                     EXPERIMENT: 05114 TEST: 02                                                 PAGE   2
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- HEXACHLOROCYCLOPENTADIENE (HCCPD)
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   | CONTROL    0.01 PPM   0.05 PPM   0.2  PPM   0.5  PP|   CONTROL      0.01 PPM     0.05 PPM     0.2   PP  |
|                       |                                  SE65       SE42   |                                          M         |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hemangiosarcoma                                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)             0/90 (0%)  1/42 (2%)  0/47 (0%)  2/91 (2%)  0/60 (0%)  0/59 (0%)    1/60 (2%)    0/60 (0%)    0/60 (0%)  |
|ADJUSTED (b)             0.0%       2.6%       0.0%       3.6%       0.0%   |   0.0%         2.0%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  0/10 (0%)  0/10 (0%)  0/10 (0%)  0/20 (0%)  0/20 (0%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)            0/35 (0%)  0/15 (0%)  0/29 (0%)  1/34 (3%)  0/0     |  0/31 (0%)    0/32 (0%)    0/30 (0%)    0/21 (0%)  |
|FIRST INCIDENCE (DAYS) | ---        687        ---        481        ---    |   ---          503          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P=0.611N   P=0.505      (e)      P=0.349      (e)   |  P=0.617N     P=0.513        (e)          (e)      |
|INCIDENTAL TUMOR       |P=0.470    P=0.527      (e)      P=0.413      (e)   |  P=0.279N     P=0.282        (e)          (e)      |
|LOGISTIC REGRESSION    |P=0.619    P=0.384      (e)      P=0.249      (e)   |  P=0.543N     P=0.471        (e)          (e)      |
|COCHRAN-ARMITAGE       |P=0.621N                                            |  P=0.597N                                          |
|FISHER EXACT           |           P=0.318      (e)      P=0.251      (e)   |               P=0.504        (e)          (e)      |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Adenoma                                                                                                      |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            23/90 (26% 16/42 (38% 23/47 (49% 11/91 (12%  1/60 (2%)  6/59 (10%)  10/60 (17%)   7/60 (12%)   6/60 (10%) |
|ADJUSTED (b)            52.8%      87.5%      65.6%      25.2%       0.0%   |  16.5%        31.3%        22.5%        21.3%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  1/10 (10%  0/0        0/0        0/10 (0%)  1/20 (5%)                                                    |
|INTERIM SACRIFICE 4 (c  3/10 (30%  2/10 (20%  2/10 (20%  1/20 (5%)  0/20 (0%)  1/10 (10%)   0/10 (0%)    0/10 (0%)    1/10 (10%) |
|TERMINAL (d)           16/35 (46% 13/15 (87% 18/29 (62%  8/34 (24%  0/0     |  4/31 (13%)  10/32 (31%)   6/30 (20%)   2/21 (10%) |
|FIRST INCIDENCE (DAYS) | 295 (I)    459 (I)    459 (I)    459 (I)    295 (I)|   458 (I)      736 (T)      718          458 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P<0.001N** P=0.139    P=0.283    P=0.002N** P=0.002N** P=0.496      P=0.222      P=0.483      P=0.417    |
|INCIDENTAL TUMOR       |P<0.001N** P=0.134    P=0.192    P=0.006N** P=0.037N*  P=0.562      P=0.227      P=0.510      P=0.527    |
|LOGISTIC REGRESSION    |P<0.001N** P=0.287    P=0.123    P=0.009N** P=0.090N   P=0.522N     P=0.221      P=0.517      P=0.558    |
|COCHRAN-ARMITAGE       |P<0.001N**                                          |  P=0.339N                                          |
|FISHER EXACT           |           P=0.104    P=0.006 ** P=0.016N*  P<0.001N**              P=0.221      P=0.513      P=0.607N   |
|=================================================================================================================================|
 
         DATE: 09/15/94                     EXPERIMENT: 05114 TEST: 02                                                 PAGE   3
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- HEXACHLOROCYCLOPENTADIENE (HCCPD)
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   | CONTROL    0.01 PPM   0.05 PPM   0.2  PPM   0.5  PP|   CONTROL      0.01 PPM     0.05 PPM     0.2   PP  |
|                       |                                  SE65       SE42   |                                          M         |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma                                                                                                    |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)             9/90 (10%  7/42 (17% 10/47 (21% 12/91 (13%  0/60 (0%)  4/59 (7%)    2/60 (3%)    4/60 (7%)    1/60 (2%)  |
|ADJUSTED (b)            20.5%      22.7%      30.7%      15.5%       0.0%   |  11.2%         5.4%        10.8%         4.8%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  2/10 (20%  0/10 (0%)  0/10 (0%)  0/20 (0%)  0/20 (0%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)            3/35 (9%)  2/15 (13%  8/29 (28%  3/34 (9%)  0/0     |  2/31 (6%)    0/32 (0%)    1/30 (3%)    1/21 (5%)  |
|FIRST INCIDENCE (DAYS) | 459 (I)    443        618        393        ---    |   634          668          625          736 (T)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P=0.003N** P=0.591    P=0.430    P=0.243N   P=0.001N** P=0.326N     P=0.328N     P=0.633N     P=0.300N   |
|INCIDENTAL TUMOR       |P=0.578    P=0.546N   P=0.231    P=0.319    P=0.102N   P=0.206N     P=0.290N     P=0.562N     P=0.222N   |
|LOGISTIC REGRESSION    |P=0.448N   P=0.576    P=0.235    P=0.374    P=0.192N   P=0.251N     P=0.326N     P=0.632N     P=0.229N   |
|COCHRAN-ARMITAGE       |P=0.005N**                                          |  P=0.195N                                          |
|FISHER EXACT           |           P=0.207    P=0.063    P=0.331    P=0.009N**              P=0.332N     P=0.632N     P=0.177N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma or Hepatocellular Adenoma                                                                          |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            30/90 (33% 23/42 (55% 30/47 (64% 21/91 (23%  1/60 (2%) 10/59 (17%)  12/60 (20%)  10/60 (17%)   7/60 (12%) |
|ADJUSTED (b)            61.2%     100.0%      82.0%      35.5%       0.0%   |  26.7%        35.0%        29.3%        25.5%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  1/10 (10%  0/0        0/0        0/10 (0%)  1/20 (5%)                                                    |
|INTERIM SACRIFICE 4 (c  5/10 (50%  2/10 (20%  2/10 (20%  1/20 (5%)  0/20 (0%)  1/10 (10%)   0/10 (0%)    0/10 (0%)    1/10 (10%) |
|TERMINAL (d)           17/35 (49% 15/15 (100 23/29 (79% 10/34 (29%  0/0     |  6/31 (19%)  10/32 (31%)   7/30 (23%)   3/21 (14%) |
|FIRST INCIDENCE (DAYS) | 295 (I)    443        459 (I)    393        295 (I)|   458 (I)      668          625          458 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P<0.001N** P=0.128    P=0.286    P=0.001N** P<0.001N** P=0.476N     P=0.436      P=0.583      P=0.557N   |
|INCIDENTAL TUMOR       |P<0.001N** P=0.128    P=0.093    P=0.032N*  P=0.004N** P=0.343N     P=0.461      P=0.546N     P=0.407N   |
|LOGISTIC REGRESSION    |P<0.001N** P=0.098    P=0.054    P=0.025N*  P=0.020N*  P=0.298N     P=0.432      P=0.578N     P=0.385N   |
|COCHRAN-ARMITAGE       |P<0.001N**                                          |  P=0.163N                                          |
|FISHER EXACT           |           P=0.016 *  P<0.001 ** P=0.085N   P<0.001N**              P=0.424      P=0.580N     P=0.288N   |
|=================================================================================================================================|
 
         DATE: 09/15/94                     EXPERIMENT: 05114 TEST: 02                                                 PAGE   4
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- HEXACHLOROCYCLOPENTADIENE (HCCPD)
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   | CONTROL    0.01 PPM   0.05 PPM   0.2  PPM   0.5  PP|   CONTROL      0.01 PPM     0.05 PPM     0.2   PP  |
|                       |                                  SE65       SE42   |                                          M         |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma                                                         |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            30/90 (33% 23/42 (55% 30/47 (64% 21/91 (23%  1/60 (2%) 10/59 (17%)  12/60 (20%)  10/60 (17%)   7/60 (12%) |
|ADJUSTED (b)            61.2%     100.0%      82.0%      35.5%       0.0%   |  26.7%        35.0%        29.3%        25.5%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  1/10 (10%  0/0        0/0        0/10 (0%)  1/20 (5%)                                                    |
|INTERIM SACRIFICE 4 (c  5/10 (50%  2/10 (20%  2/10 (20%  1/20 (5%)  0/20 (0%)  1/10 (10%)   0/10 (0%)    0/10 (0%)    1/10 (10%) |
|TERMINAL (d)           17/35 (49% 15/15 (100 23/29 (79% 10/34 (29%  0/0     |  6/31 (19%)  10/32 (31%)   7/30 (23%)   3/21 (14%) |
|FIRST INCIDENCE (DAYS) | 295 (I)    443        459 (I)    393        295 (I)|   458 (I)      668          625          458 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P<0.001N** P=0.128    P=0.286    P=0.001N** P<0.001N** P=0.476N     P=0.436      P=0.583      P=0.557N   |
|INCIDENTAL TUMOR       |P<0.001N** P=0.128    P=0.093    P=0.032N*  P=0.004N** P=0.343N     P=0.461      P=0.546N     P=0.407N   |
|LOGISTIC REGRESSION    |P<0.001N** P=0.098    P=0.054    P=0.025N*  P=0.020N*  P=0.298N     P=0.432      P=0.578N     P=0.385N   |
|COCHRAN-ARMITAGE       |P<0.001N**                                          |  P=0.163N                                          |
|FISHER EXACT           |           P=0.016 *  P<0.001 ** P=0.085N   P<0.001N**              P=0.424      P=0.580N     P=0.288N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Adenoma                                                                                                |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            13/89 (15% 10/60 (17% 11/60 (18% 41/189 (22 22/160 (14  4/58 (7%)    3/60 (5%)    4/60 (7%)    5/59 (8%)  |
|ADJUSTED (b)            33.4%      29.2%      24.4%      35.2%      27.9%   |  12.9%         9.4%        11.6%        16.1%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  1/10 (10%  0/0        0/0        1/10 (10%  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  1/10 (10%  0/10 (0%)  1/10 (10%  1/20 (5%)  2/20 (10%  0/10 (0%)    0/10 (0%)    1/10 (10%)   1/10 (10%) |
|TERMINAL (d)           10/34 (29%  9/33 (27%  9/42 (21% 30/102 (29 19/74 (26%  4/31 (13%)   3/32 (9%)    3/30 (10%)   1/21 (5%)  |
|FIRST INCIDENCE (DAYS) | 295 (I)    689        459 (I)    295 (I)    458 (I)|   736 (T)      736 (T)      458 (I)      458 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P=0.388N   P=0.431N   P=0.311N   P=0.399    P=0.286N   P=0.170      P=0.482N     P=0.628      P=0.323    |
|INCIDENTAL TUMOR       |P=0.472N   P=0.418N   P=0.363N   P=0.424    P=0.325N   P=0.208      P=0.482N     P=0.628      P=0.424    |
|LOGISTIC REGRESSION    |P=0.505N   P=0.348N   P=0.395N   P=0.401    P=0.392N   P=0.270      P=0.482N     P=0.624N     P=0.435    |
|COCHRAN-ARMITAGE       |P=0.264N                                            |  P=0.354                                           |
|FISHER EXACT           |           P=0.452    P=0.349    P=0.108    P=0.496N                P=0.481N     P=0.622N     P=0.511    |
|=================================================================================================================================|
 
         DATE: 09/15/94                     EXPERIMENT: 05114 TEST: 02                                                 PAGE   5
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- HEXACHLOROCYCLOPENTADIENE (HCCPD)
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   | CONTROL    0.01 PPM   0.05 PPM   0.2  PPM   0.5  PP|   CONTROL      0.01 PPM     0.05 PPM     0.2   PP  |
|                       |                                  SE65       SE42   |                                          M         |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Carcinoma                                                                                              |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)             0/89 (0%)  3/60 (5%)  4/60 (7%)  8/189 (4% 12/160 (8%  3/58 (5%)    1/60 (2%)    2/60 (3%)    1/59 (2%)  |
|ADJUSTED (b)             0.0%       7.7%       9.5%       7.0%      14.7%   |   8.3%         3.1%         5.6%         4.8%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  0/10 (0%)  1/10 (10%  0/10 (0%)  1/20 (5%)  1/20 (5%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)            0/34 (0%)  2/33 (6%)  4/42 (10%  5/102 (5%  8/74 (11%  1/31 (3%)    1/32 (3%)    1/30 (3%)    1/21 (5%)  |
|FIRST INCIDENCE (DAYS) | ---        459 (I)    729 (T)    459 (I)    395    |   620          736 (T)      604          736 (T)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P=0.017 *  P=0.119    P=0.093    P=0.098    P=0.017 *  P=0.503N     P=0.305N     P=0.504N     P=0.436N   |
|INCIDENTAL TUMOR       |P=0.013 *  P=0.119    P=0.093    P=0.113    P=0.013 *  P=0.428N     P=0.266N     P=0.424N     P=0.326N   |
|LOGISTIC REGRESSION    |P=0.014 *  P=0.090    P=0.093    P=0.087    P=0.012 *  P=0.427N     P=0.290N     P=0.484N     P=0.357N   |
|COCHRAN-ARMITAGE       |P=0.034 *                                           |  P=0.369N                                          |
|FISHER EXACT           |           P=0.063    P=0.025 *  P=0.043 *  P=0.004 **              P=0.297N     P=0.484N     P=0.303N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma                                                              |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            13/89 (15% 12/60 (20% 15/60 (25% 49/189 (26 31/160 (19  7/58 (12%)   4/60 (7%)    6/60 (10%)   6/59 (10%) |
|ADJUSTED (b)            33.4%      33.3%      33.5%      41.0%      37.4%   |  20.5%        12.5%        16.8%        20.3%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  1/10 (10%  0/0        0/0        1/10 (10%  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  1/10 (10%  1/10 (10%  1/10 (10%  2/20 (10%  3/20 (15%  0/10 (0%)    0/10 (0%)    1/10 (10%)   1/10 (10%) |
|TERMINAL (d)           10/34 (29% 10/33 (30% 13/42 (31% 35/102 (34 24/74 (32%  5/31 (16%)   4/32 (13%)   4/30 (13%)   2/21 (10%) |
|FIRST INCIDENCE (DAYS) | 295 (I)    459 (I)    459 (I)    295 (I)    395    |   620          736 (T)      458 (I)      458 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P=0.338    P=0.565    P=0.547    P=0.174    P=0.421    P=0.278      P=0.249N     P=0.516N     P=0.498    |
|INCIDENTAL TUMOR       |P=0.251    P=0.578    P=0.492    P=0.198    P=0.366    P=0.358      P=0.226N     P=0.465N     P=0.579N   |
|LOGISTIC REGRESSION    |P=0.234    P=0.551N   P=0.453    P=0.165    P=0.299    P=0.421      P=0.238N     P=0.472N     P=0.589N   |
|COCHRAN-ARMITAGE       |P=0.518                                             |  P=0.532                                           |
|FISHER EXACT           |           P=0.259    P=0.085    P=0.023 *  P=0.221                 P=0.245N     P=0.474N     P=0.487N   |
|=================================================================================================================================|
 
         DATE: 09/15/94                     EXPERIMENT: 05114 TEST: 02                                                 PAGE   6
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- HEXACHLOROCYCLOPENTADIENE (HCCPD)
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   | CONTROL    0.01 PPM   0.05 PPM   0.2  PPM   0.5  PP|   CONTROL      0.01 PPM     0.05 PPM     0.2   PP  |
|                       |                                  SE65       SE42   |                                          M         |
|=================================================================================================================================|
|                                                                                                                                 |
|Ovary                                                                                                                            |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |  1/59 (2%)    0/60 (0%)    2/60 (3%)    0/60 (0%)  |
|ADJUSTED (b)           |                                                    |   3.2%         0.0%         6.7%         0.0%      |
|INTERIM SACRIFICE 1 (c)|                                                    |                                                    |
|INTERIM SACRIFICE 2 (c)|                                                    |                                                    |
|INTERIM SACRIFICE 3 (c)|                                                    |                                                    |
|INTERIM SACRIFICE 4 (c)|                                                    |  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)           |                                                    |  1/31 (3%)    0/32 (0%)    2/30 (7%)    0/21 (0%)  |
|FIRST INCIDENCE (DAYS) |                                                    |   736 (T)      ---          736 (T)      ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |  P=0.553N     P=0.494N     P=0.488      P=0.578N   |
|INCIDENTAL TUMOR       |                                                    |  P=0.553N     P=0.494N     P=0.488      P=0.578N   |
|LOGISTIC REGRESSION    |                                                    |  P=0.553N     P=0.494N     P=0.488      P=0.578N   |
|COCHRAN-ARMITAGE       |                                                    |  P=0.440N                                          |
|FISHER EXACT           |                                                    |               P=0.496N     P=0.506      P=0.496N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Pituitary Gland: Pars Distalis or Unspecified Site                                                                               |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)             0/89 (0%)  0/26 (0%)  0/18 (0%)  0/89 (0%)  0/60 (0%)  8/59 (14%)   3/59 (5%)    5/58 (9%)    3/60 (5%)  |
|ADJUSTED (b)             0.0%       0.0%       0.0%       0.0%       0.0%   |  22.0%         8.8%        15.6%        14.3%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  0/10 (0%)  0/10 (0%)  0/10 (0%)  0/20 (0%)  0/20 (0%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)            0/35 (0%)  0/0        0/0        0/34 (0%)  0/0     |  4/31 (13%)   2/31 (6%)    4/30 (13%)   3/21 (14%) |
|FIRST INCIDENCE (DAYS) | ---        ---        ---        ---        ---    |   589          646          654          736 (T)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  (e)        (e)        (e)        (e)        (e)   |  P=0.399N     P=0.107N     P=0.293N     P=0.242N   |
|INCIDENTAL TUMOR       |  (e)        (e)        (e)        (e)        (e)   |  P=0.316N     P=0.081N     P=0.275N     P=0.162N   |
|LOGISTIC REGRESSION    |  (e)        (e)        (e)        (e)        (e)   |  P=0.297N     P=0.099N     P=0.284N     P=0.165N   |
|COCHRAN-ARMITAGE       |  (e)                                               |  P=0.193N                                          |
|FISHER EXACT           |             (e)        (e)        (e)        (e)   |               P=0.102N     P=0.290N     P=0.097N   |
|=================================================================================================================================|
 
         DATE: 09/15/94                     EXPERIMENT: 05114 TEST: 02                                                 PAGE   7
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- HEXACHLOROCYCLOPENTADIENE (HCCPD)
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   | CONTROL    0.01 PPM   0.05 PPM   0.2  PPM   0.5  PP|   CONTROL      0.01 PPM     0.05 PPM     0.2   PP  |
|                       |                                  SE65       SE42   |                                          M         |
|=================================================================================================================================|
|                                                                                                                                 |
|Pituitary Gland: Pars Distalis or Unspecified Site                                                                               |
|     Carcinoma or Adenoma                                                                                                        |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)             1/89 (1%)  0/26 (0%)  0/18 (0%)  0/89 (0%)  0/60 (0%)  8/59 (14%)   4/59 (7%)    6/58 (10%)   3/60 (5%)  |
|ADJUSTED (b)             2.9%       0.0%       0.0%       0.0%       0.0%   |  22.0%        10.8%        17.5%        14.3%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  0/10 (0%)  0/10 (0%)  0/10 (0%)  0/20 (0%)  0/20 (0%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)            1/35 (3%)  0/0        0/0        0/34 (0%)  0/0     |  4/31 (13%)   2/31 (6%)    4/30 (13%)   3/21 (14%) |
|FIRST INCIDENCE (DAYS) | 729 (T)    ---        ---        ---        ---    |   589          576          592          736 (T)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P=0.697N     (e)        (e)      P=0.506N     (e)   |  P=0.337N     P=0.186N     P=0.401N     P=0.242N   |
|INCIDENTAL TUMOR       |P=0.697N     (e)        (e)      P=0.506N     (e)   |  P=0.236N     P=0.147N     P=0.373N     P=0.162N   |
|LOGISTIC REGRESSION    |P=0.697N     (e)        (e)      P=0.506N     (e)   |  P=0.225N     P=0.176N     P=0.398N     P=0.165N   |
|COCHRAN-ARMITAGE       |P=0.450N                                            |  P=0.151N                                          |
|FISHER EXACT           |           P=0.774N   P=0.832N   P=0.500N   P=0.597N                P=0.181N     P=0.402N     P=0.097N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Spleen                                                                                                                           |
|     Hemangiosarcoma                                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)             0/90 (0%)  0/28 (0%)  0/23 (0%)  0/90 (0%)  0/60 (0%)  0/59 (0%)    2/60 (3%)    0/60 (0%)    0/60 (0%)  |
|ADJUSTED (b)             0.0%       0.0%       0.0%       0.0%       0.0%   |   0.0%         4.4%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  0/10 (0%)  0/10 (0%)  0/10 (0%)  0/20 (0%)  0/20 (0%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)            0/35 (0%)  0/1 (0%)   0/5 (0%)   0/34 (0%)  0/0     |  0/31 (0%)    0/32 (0%)    0/30 (0%)    0/21 (0%)  |
|FIRST INCIDENCE (DAYS) | ---        ---        ---        ---        ---    |   ---          503          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  (e)        (e)        (e)        (e)        (e)   |  P=0.421N     P=0.249        (e)          (e)      |
|INCIDENTAL TUMOR       |  (e)        (e)        (e)        (e)        (e)   |  P=0.169N     P=0.139        (e)          (e)      |
|LOGISTIC REGRESSION    |  (e)        (e)        (e)        (e)        (e)   |  P=0.343N     P=0.227        (e)          (e)      |
|COCHRAN-ARMITAGE       |  (e)                                               |  P=0.377N                                          |
|FISHER EXACT           |             (e)        (e)        (e)        (e)   |               P=0.252        (e)          (e)      |
|=================================================================================================================================|
 
         DATE: 09/15/94                     EXPERIMENT: 05114 TEST: 02                                                 PAGE   8
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- HEXACHLOROCYCLOPENTADIENE (HCCPD)
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   | CONTROL    0.01 PPM   0.05 PPM   0.2  PPM   0.5  PP|   CONTROL      0.01 PPM     0.05 PPM     0.2   PP  |
|                       |                                  SE65       SE42   |                                          M         |
|=================================================================================================================================|
|                                                                                                                                 |
|Stomach, Forestomach                                                                                                             |
|     Squamous Cell Papilloma                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |  #          #          #          #          #     |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)             0/90 (0%)  1/60 (2%)  1/60 (2%)  1/190 (1%  0/160 (0%  0/60 (0%)    0/60 (0%)    1/60 (2%)    2/60 (3%)  |
|ADJUSTED (b)             0.0%       1.7%       2.4%       1.0%       0.0%   |   0.0%         0.0%         3.3%         9.5%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  0/10 (0%)  0/10 (0%)  0/10 (0%)  0/20 (0%)  0/20 (0%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)            0/35 (0%)  0/33 (0%)  1/42 (2%)  1/102 (1%  0/74 (0%)  0/31 (0%)    0/32 (0%)    1/30 (3%)    2/21 (10%) |
|FIRST INCIDENCE (DAYS) | ---        443        729 (T)    729 (T)    ---    |   ---          ---          736 (T)      736 (T)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P=0.258N   P=0.500    P=0.536    P=0.712      (e)   |  P=0.041 *      (e)        P=0.493      P=0.157    |
|INCIDENTAL TUMOR       |P=0.236N   P=0.773    P=0.536    P=0.712      (e)   |  P=0.041 *      (e)        P=0.493      P=0.157    |
|LOGISTIC REGRESSION    |P=0.248N   P=0.304    P=0.536    P=0.712      (e)   |  P=0.041 *      (e)        P=0.493      P=0.157    |
|COCHRAN-ARMITAGE       |P=0.237N                                            |  P=0.096                                           |
|FISHER EXACT           |           P=0.400    P=0.400    P=0.679      (e)   |                 (e)        P=0.500      P=0.248    |
|=================================================================================================================================|
|                                                                                                                                 |
|Thyroid Gland: Follicular Cell                                                                                                   |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)             1/88 (1%)  0/29 (0%)  3/22 (14%  6/182 (3%  1/149 (1%  1/59 (2%)    1/60 (2%)    6/60 (10%)   0/60 (0%)  |
|ADJUSTED (b)             2.9%       0.0%      51.1%       5.7%       1.4%   |   2.6%         3.1%        18.2%         0.0%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  0/10 (0%)  0/10 (0%)  0/10 (0%)  0/20 (0%)  0/20 (0%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)            1/34 (3%)  0/2 (0%)   2/4 (50%)  4/100 (4%  1/74 (1%)  0/31 (0%)    1/32 (3%)    4/30 (13%)   0/21 (0%)  |
|FIRST INCIDENCE (DAYS) | 729 (T)    ---        618        654        729 (T)|   657          736 (T)      660          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P=0.106N   P=0.974N   P=0.010 ** P=0.405    P=0.579N   P=0.409N     P=0.754N     P=0.061      P=0.554N   |
|INCIDENTAL TUMOR       |P=0.131N   P=0.974N   P=0.010 ** P=0.379    P=0.579N   P=0.352N     P=0.742N     P=0.072      P=0.473N   |
|LOGISTIC REGRESSION    |P=0.105N   P=0.974N   P=0.008 ** P=0.397    P=0.579N   P=0.349N     P=0.756N     P=0.061      P=0.509N   |
|COCHRAN-ARMITAGE       |P=0.162N                                            |  P=0.269N                                          |
|FISHER EXACT           |           P=0.752N   P=0.025 *  P=0.274    P=0.606N                P=0.748N     P=0.060      P=0.496N   |
|=================================================================================================================================|
 
         DATE: 09/15/94                     EXPERIMENT: 05114 TEST: 02                                                 PAGE   9
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- HEXACHLOROCYCLOPENTADIENE (HCCPD)
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   | CONTROL    0.01 PPM   0.05 PPM   0.2  PPM   0.5  PP|   CONTROL      0.01 PPM     0.05 PPM     0.2   PP  |
|                       |                                  SE65       SE42   |                                          M         |
|=================================================================================================================================|
|                                                                                                                                 |
|Uterus                                                                                                                           |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |  1/60 (2%)    0/60 (0%)    2/60 (3%)    0/60 (0%)  |
|ADJUSTED (b)           |                                                    |   3.2%         0.0%         5.8%         0.0%      |
|INTERIM SACRIFICE 1 (c)|                                                    |                                                    |
|INTERIM SACRIFICE 2 (c)|                                                    |                                                    |
|INTERIM SACRIFICE 3 (c)|                                                    |                                                    |
|INTERIM SACRIFICE 4 (c)|                                                    |  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)           |                                                    |  1/31 (3%)    0/32 (0%)    0/30 (0%)    0/21 (0%)  |
|FIRST INCIDENCE (DAYS) |                                                    |   736 (T)      ---          674          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |  P=0.530N     P=0.494N     P=0.503      P=0.578N   |
|INCIDENTAL TUMOR       |                                                    |  P=0.467N     P=0.494N     P=0.555      P=0.578N   |
|LOGISTIC REGRESSION    |                                                    |  P=0.500N     P=0.494N     P=0.507      P=0.578N   |
|COCHRAN-ARMITAGE       |                                                    |  P=0.442N                                          |
|FISHER EXACT           |                                                    |               P=0.500N     P=0.500      P=0.500N   |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangioma                                                                                                                  |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |  #          #          #          #          #     |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)             0/90 (0%)  0/60 (0%)  0/60 (0%)  0/190 (0%  0/160 (0%  1/60 (2%)    2/60 (3%)    1/60 (2%)    0/60 (0%)  |
|ADJUSTED (b)             0.0%       0.0%       0.0%       0.0%       0.0%   |   2.3%         5.7%         3.3%         0.0%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  0/10 (0%)  0/10 (0%)  0/10 (0%)  0/20 (0%)  0/20 (0%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)            0/35 (0%)  0/33 (0%)  0/42 (0%)  0/102 (0%  0/74 (0%)  0/31 (0%)    1/32 (3%)    1/30 (3%)    0/21 (0%)  |
|FIRST INCIDENCE (DAYS) | ---        ---        ---        ---        ---    |   598          668          736 (T)      ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  (e)        (e)        (e)        (e)        (e)   |  P=0.298N     P=0.504      P=0.757      P=0.530N   |
|INCIDENTAL TUMOR       |  (e)        (e)        (e)        (e)        (e)   |  P=0.254N     P=0.534      P=0.737N     P=0.429N   |
|LOGISTIC REGRESSION    |  (e)        (e)        (e)        (e)        (e)   |  P=0.257N     P=0.506      P=0.760N     P=0.493N   |
|COCHRAN-ARMITAGE       |  (e)                                               |  P=0.235N                                          |
|FISHER EXACT           |             (e)        (e)        (e)        (e)   |               P=0.500      P=0.752N     P=0.500N   |
|=================================================================================================================================|
 
         DATE: 09/15/94                     EXPERIMENT: 05114 TEST: 02                                                 PAGE  10
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- HEXACHLOROCYCLOPENTADIENE (HCCPD)
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   | CONTROL    0.01 PPM   0.05 PPM   0.2  PPM   0.5  PP|   CONTROL      0.01 PPM     0.05 PPM     0.2   PP  |
|                       |                                  SE65       SE42   |                                          M         |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangiosarcoma                                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |  #          #          #          #          #     |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)             0/90 (0%)  1/60 (2%)  1/60 (2%)  2/190 (1%  0/160 (0%  0/60 (0%)    2/60 (3%)    0/60 (0%)    1/60 (2%)  |
|ADJUSTED (b)             0.0%       2.6%       2.4%       1.7%       0.0%   |   0.0%         4.4%         0.0%         3.8%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  0/10 (0%)  0/10 (0%)  0/10 (0%)  0/20 (0%)  0/20 (0%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)            0/35 (0%)  0/33 (0%)  1/42 (2%)  1/102 (1%  0/74 (0%)  0/31 (0%)    0/32 (0%)    0/30 (0%)    0/21 (0%)  |
|FIRST INCIDENCE (DAYS) | ---        687        729 (T)    481        ---    |   ---          503          ---          695       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P=0.281N   P=0.505    P=0.536    P=0.497      (e)   |  P=0.588      P=0.249        (e)        P=0.459    |
|INCIDENTAL TUMOR       |P=0.286N   P=0.527    P=0.536    P=0.638      (e)   |  P=0.546N     P=0.139        (e)        P=0.527    |
|LOGISTIC REGRESSION    |P=0.275N   P=0.486    P=0.536    P=0.429      (e)   |  P=0.654      P=0.211        (e)        P=0.470    |
|COCHRAN-ARMITAGE       |P=0.253N                                            |  P=0.641                                           |
|FISHER EXACT           |           P=0.400    P=0.400    P=0.460      (e)   |               P=0.248        (e)        P=0.500    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangiosarcoma or Hemangioma                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |  #          #          #          #          #     |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)             0/90 (0%)  1/60 (2%)  1/60 (2%)  2/190 (1%  0/160 (0%  1/60 (2%)    4/60 (7%)    1/60 (2%)    1/60 (2%)  |
|ADJUSTED (b)             0.0%       2.6%       2.4%       1.7%       0.0%   |   2.3%         9.9%         3.3%         3.8%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  0/10 (0%)  0/10 (0%)  0/10 (0%)  0/20 (0%)  0/20 (0%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)            0/35 (0%)  0/33 (0%)  1/42 (2%)  1/102 (1%  0/74 (0%)  0/31 (0%)    1/32 (3%)    1/30 (3%)    0/21 (0%)  |
|FIRST INCIDENCE (DAYS) | ---        687        729 (T)    481        ---    |   598          503          736 (T)      695       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P=0.281N   P=0.505    P=0.536    P=0.497      (e)   |  P=0.434N     P=0.193      P=0.757      P=0.720    |
|INCIDENTAL TUMOR       |P=0.286N   P=0.527    P=0.536    P=0.638      (e)   |  P=0.223N     P=0.143      P=0.737N     P=0.705N   |
|LOGISTIC REGRESSION    |P=0.275N   P=0.486    P=0.536    P=0.429      (e)   |  P=0.346N     P=0.204      P=0.760N     P=0.755    |
|COCHRAN-ARMITAGE       |P=0.253N                                            |  P=0.333N                                          |
|FISHER EXACT           |           P=0.400    P=0.400    P=0.460      (e)   |               P=0.182      P=0.752N     P=0.752N   |
|=================================================================================================================================|
 
         DATE: 09/15/94                     EXPERIMENT: 05114 TEST: 02                                                 PAGE  11
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- HEXACHLOROCYCLOPENTADIENE (HCCPD)
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   | CONTROL    0.01 PPM   0.05 PPM   0.2  PPM   0.5  PP|   CONTROL      0.01 PPM     0.05 PPM     0.2   PP  |
|                       |                                  SE65       SE42   |                                          M         |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Histiocytic Sarcoma                                                                                                         |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |  #          #          #          #          #     |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)             0/90 (0%)  0/60 (0%)  0/60 (0%)  0/190 (0%  0/160 (0%  4/60 (7%)    1/60 (2%)    0/60 (0%)    0/60 (0%)  |
|ADJUSTED (b)             0.0%       0.0%       0.0%       0.0%       0.0%   |   9.2%         2.9%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  0/10 (0%)  0/10 (0%)  0/10 (0%)  0/20 (0%)  0/20 (0%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)            0/35 (0%)  0/33 (0%)  0/42 (0%)  0/102 (0%  0/74 (0%)  0/31 (0%)    0/32 (0%)    0/30 (0%)    0/21 (0%)  |
|FIRST INCIDENCE (DAYS) | ---        ---        ---        ---        ---    |   396          688          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  (e)        (e)        (e)        (e)        (e)   |  P=0.105N     P=0.184N     P=0.065N     P=0.083N   |
|INCIDENTAL TUMOR       |  (e)        (e)        (e)        (e)        (e)   |  P=0.015N*    P=0.262N     P=0.091N     P=0.009N** |
|LOGISTIC REGRESSION    |  (e)        (e)        (e)        (e)        (e)   |  P=0.077N     P=0.200N     P=0.066N     P=0.053N   |
|COCHRAN-ARMITAGE       |  (e)                                               |  P=0.088N                                          |
|FISHER EXACT           |             (e)        (e)        (e)        (e)   |               P=0.182N     P=0.059N     P=0.059N   |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Lymphoma And Histiocytic Sarcoma                                                                                  |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |  #          #          #          #          #     |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)             2/90 (2%)  5/60 (8%)  5/60 (8%) 10/190 (5%  2/160 (1% 15/60 (25%)  11/60 (18%)   5/60 (8%)    9/60 (15%) |
|ADJUSTED (b)             4.9%      13.2%      10.1%       8.3%       2.5%   |  36.3%        28.2%        13.7%        28.9%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  0/10 (0%)  0/10 (0%)  1/10 (10%  0/20 (0%)  0/20 (0%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)            0/35 (0%)  2/33 (6%)  1/42 (2%)  3/102 (3%  1/74 (1%)  7/31 (23%)   5/32 (16%)   2/30 (7%)    3/21 (14%) |
|FIRST INCIDENCE (DAYS) | 627        617        459 (I)    435        612    |   396          577          625          400       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P=0.051N   P=0.207    P=0.274    P=0.351    P=0.436N   P=0.493N     P=0.253N     P=0.019N*    P=0.337N   |
|INCIDENTAL TUMOR       |P=0.096N   P=0.203    P=0.081    P=0.398    P=0.663N   P=0.064N     P=0.279N     P=0.015N*    P=0.039N*  |
|LOGISTIC REGRESSION    |P=0.077N   P=0.190    P=0.163    P=0.358    P=0.448N   P=0.266N     P=0.236N     P=0.013N*    P=0.148N   |
|COCHRAN-ARMITAGE       |P=0.030N*                                           |  P=0.215N                                          |
|FISHER EXACT           |           P=0.091    P=0.091    P=0.199    P=0.455N                P=0.253N     P=0.013N*    P=0.127N   |
|=================================================================================================================================|
 
         DATE: 09/15/94                     EXPERIMENT: 05114 TEST: 02                                                 PAGE  12
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- HEXACHLOROCYCLOPENTADIENE (HCCPD)
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   | CONTROL    0.01 PPM   0.05 PPM   0.2  PPM   0.5  PP|   CONTROL      0.01 PPM     0.05 PPM     0.2   PP  |
|                       |                                  SE65       SE42   |                                          M         |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Lymphoma: Histiocytic, Lymphocytic, Mixed,                                                                        |
|     NOS, or Undifferentiated Cell Type                                                                                          |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |  #          #          #          #          #     |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)             2/90 (2%)  5/60 (8%)  5/60 (8%) 10/190 (5%  2/160 (1% 13/60 (22%)  10/60 (17%)   5/60 (8%)    9/60 (15%) |
|ADJUSTED (b)             4.9%      13.2%      10.1%       8.3%       2.5%   |  33.8%        26.0%        13.7%        28.9%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  0/10 (0%)  0/10 (0%)  1/10 (10%  0/20 (0%)  0/20 (0%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)            0/35 (0%)  2/33 (6%)  1/42 (2%)  3/102 (3%  1/74 (1%)  7/31 (23%)   5/32 (16%)   2/30 (7%)    3/21 (14%) |
|FIRST INCIDENCE (DAYS) | 627        617        459 (I)    435        612    |   520          577          625          400       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P=0.051N   P=0.207    P=0.274    P=0.351    P=0.436N   P=0.483      P=0.313N     P=0.045N*    P=0.503N   |
|INCIDENTAL TUMOR       |P=0.096N   P=0.203    P=0.081    P=0.398    P=0.663N   P=0.220N     P=0.285N     P=0.028N*    P=0.157N   |
|LOGISTIC REGRESSION    |P=0.077N   P=0.190    P=0.163    P=0.358    P=0.448N   P=0.437N     P=0.281N     P=0.026N*    P=0.301N   |
|COCHRAN-ARMITAGE       |P=0.030N*                                           |  P=0.344N                                          |
|FISHER EXACT           |           P=0.091    P=0.091    P=0.199    P=0.455N                P=0.322N     P=0.036N*    P=0.240N   |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Benign Tumors                                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |  #          #          #          #          #     |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            36/90 (40% 25/60 (42% 34/60 (57% 60/190 (32 27/160 (17 24/60 (40%)  22/60 (37%)  25/60 (42%)  15/60 (25%) |
|ADJUSTED (b)            75.3%      64.5%      68.9%      49.9%      33.7%   |  59.1%        62.4%        67.0%        49.0%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  2/10 (20%  0/0        0/0        1/10 (10%  1/20 (5%)                                                    |
|INTERIM SACRIFICE 4 (c  5/10 (50%  2/10 (20%  3/10 (30%  2/20 (10%  2/20 (10%  1/10 (10%)   0/10 (0%)    1/10 (10%)   2/10 (20%) |
|TERMINAL (d)           24/35 (69% 20/33 (61% 27/42 (64% 44/102 (43 23/74 (31% 15/31 (48%)  19/32 (59%)  18/30 (60%)   7/21 (33%) |
|FIRST INCIDENCE (DAYS) | 295 (I)    443        459 (I)    295 (I)    295 (I)|   458 (I)      633          458 (I)      458 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P<0.001N** P=0.107N   P=0.237N   P=0.003N** P<0.001N** P=0.404N     P=0.382N     P=0.470      P=0.351N   |
|INCIDENTAL TUMOR       |P<0.001N** P=0.080N   P=0.361N   P=0.002N** P<0.001N** P=0.214N     P=0.321N     P=0.565N     P=0.136N   |
|LOGISTIC REGRESSION    |P<0.001N** P=0.083N   P=0.509N   P=0.001N** P<0.001N** P=0.143N     P=0.370N     P=0.546      P=0.124N   |
|COCHRAN-ARMITAGE       |P<0.001N**                                          |  P=0.040N*                                         |
|FISHER EXACT           |           P=0.486    P=0.033 *  P=0.106N   P<0.001N**              P=0.426N     P=0.500      P=0.059N   |
|=================================================================================================================================|
 
         DATE: 09/15/94                     EXPERIMENT: 05114 TEST: 02                                                 PAGE  13
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- HEXACHLOROCYCLOPENTADIENE (HCCPD)
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   | CONTROL    0.01 PPM   0.05 PPM   0.2  PPM   0.5  PP|   CONTROL      0.01 PPM     0.05 PPM     0.2   PP  |
|                       |                                  SE65       SE42   |                                          M         |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Tumors                                                                                                            |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |  #          #          #          #          #     |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            13/90 (14% 15/60 (25% 20/60 (33% 30/190 (16 14/160 (9% 21/60 (35%)  22/60 (37%)  11/60 (18%)  12/60 (20%) |
|ADJUSTED (b)            29.1%      33.9%      40.4%      23.7%      17.0%   |  47.6%        48.7%        27.2%        39.3%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/20 (0%)                                                    |
|INTERIM SACRIFICE 4 (c  2/10 (20%  1/10 (10%  1/10 (10%  1/20 (5%)  1/20 (5%)  0/10 (0%)    0/10 (0%)    0/10 (0%)    0/10 (0%)  |
|TERMINAL (d)            5/35 (14%  6/33 (18% 13/42 (31% 12/102 (12  9/74 (12%  9/31 (29%)   9/32 (28%)   4/30 (13%)   5/21 (24%) |
|FIRST INCIDENCE (DAYS) | 459 (I)    443        459 (I)    393        395    |   396          503          411          400       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P=0.003N** P=0.385    P=0.244    P=0.327N   P=0.060N   P=0.219N     P=0.533      P=0.048N*    P=0.256N   |
|INCIDENTAL TUMOR       |P=0.006N** P=0.448    P=0.046 *  P=0.225N   P=0.133N   P=0.002N**   P=0.471      P=0.023N*    P=0.013N*  |
|LOGISTIC REGRESSION    |P=0.005N** P=0.380    P=0.078    P=0.280N   P=0.079N   P=0.028N*    P=0.570N     P=0.021N*    P=0.072N   |
|COCHRAN-ARMITAGE       |P<0.001N**                                          |  P=0.030N*                                         |
|FISHER EXACT           |           P=0.080    P=0.006 ** P=0.460    P=0.120N                P=0.500      P=0.031N*    P=0.051N   |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant and Benign Tumors                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |  #          #          #          #          #     |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            44/90 (49% 35/60 (58% 43/60 (72% 81/190 (43 37/160 (23 35/60 (58%)  37/60 (62%)  34/60 (57%)  22/60 (37%) |
|ADJUSTED (b)            82.5%      77.0%      79.5%      61.0%      43.5%   |  73.9%        80.3%        78.5%        62.2%      |
|INTERIM SACRIFICE 1 (c  0/10 (0%)  0/0        0/0        0/0        0/10 (0%)                                                    |
|INTERIM SACRIFICE 2 (c  0/10 (0%)  0/0        0/0        0/10 (0%)  0/10 (0%)                                                    |
|INTERIM SACRIFICE 3 (c  2/10 (20%  0/0        0/0        1/10 (10%  1/20 (5%)                                                    |
|INTERIM SACRIFICE 4 (c  7/10 (70%  3/10 (30%  4/10 (40%  3/20 (15%  3/20 (15%  1/10 (10%)   0/10 (0%)    1/10 (10%)   2/10 (20%) |
|TERMINAL (d)           26/35 (74% 23/33 (70% 31/42 (74% 52/102 (51 28/74 (38% 19/31 (61%)  23/32 (72%)  21/30 (70%)   9/21 (43%) |
|FIRST INCIDENCE (DAYS) | 295 (I)    443        459 (I)    295 (I)    295 (I)|   396          340          411          400       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |P<0.001N** P=0.238N   P=0.252N   P=0.005N** P<0.001N** P=0.216N     P=0.494      P=0.522N     P=0.264N   |
|INCIDENTAL TUMOR       |P<0.001N** P=0.149N   P=0.566    P<0.001N** P<0.001N** P=0.001N**   P=0.407      P=0.482N     P=0.007N** |
|LOGISTIC REGRESSION    |P<0.001N** P=0.100N   P=0.498N   P<0.001N** P<0.001N** P=0.009N**   P=0.495      P=0.447N     P=0.027N*  |
|COCHRAN-ARMITAGE       |P<0.001N**                                          |  P=0.002N**                                        |
|FISHER EXACT           |           P=0.167    P=0.004 ** P=0.196N   P<0.001N**              P=0.426      P=0.500N     P=0.014N*  |
|=================================================================================================================================|
 
         DATE: 09/15/94                     EXPERIMENT: 05114 TEST: 02                                                 PAGE  14
                      STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- HEXACHLOROCYCLOPENTADIENE (HCCPD)
 
                                                 MALE TERMINAL SACRIFICE AT 105 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 106 WEEKS
 
 
 (a)  Number of tumor-bearing animals / number of animals examined at site.
 (b)  Kaplan-Meier estimated lifetime tumor incidence after adjustment for 
      intercurrent mortality.
 (c)  Observed incidence at interim kill.
          Male interim sacrifices(days):   183  232  295  458
          Female interim sacrifices(days): 458
 (d)  Observed incidence at terminal kill.
 (f)  Beneath the control incidence are the P-values associated with the trend 
      test.  Beneath the dosed group incidence are the P-values corresponding to 
      pairwise comparisons between the controls and that dosed group.  The life
      table analysis regards tumors in animals dying  prior to terminal kill as
      being (directly or indirectly) the cause of death.  The incidental tumor 
      test regards these lesions as non-fatal.  Logistic regression is an alternative
      method for analyzing the incidence of non-fatal tumors.  The Cochran-Armitage
      and Fisher's exact tests compare directly the overall incidence rates.  
      For all tests, a negative trend is indicated by "N".
 (e)  Value of Statistic cannot be computed. 
 (I)  Interim sacrifice
 (T)  Terminal sacrifice
  #   Tumor rates based on number of animals necropsied.
 
  *   To the right of any statistical result, indicates significance at (P<=0.05).
  **  To the right of any statistical result, indicates significance at (P<=0.01).
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.