Share This:
https://ntp.niehs.nih.gov/go/3346

TDMS Study 05122-06 Pathology Tables

NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: LITRI                                             NICKEL SULFATE HEXAHYDRATE                              DATE: 05/20/95       
EXPERIMENT: 05122 TEST: 06                                                                                     TIME: 04:06:44       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                   PAGE:       1        
CONT: Y01ES30108                                ROUTE: RESPIRATORY EXPOSURE WHOLE BODY                         NTP C#: C60344       
PATHOLOGIST: F. HAHN                                                                                           CAS: 010101970       
------------------------------------------------------------------------------------------------------------------------------------
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
               REASONS FOR REMOVAL:    ALL                                                                                          
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
               REMOVAL DATE RANGE:     ALL                                                                                          
                                                                                                                                    
               TREATMENT GROUPS:       INCLUDE ALL                                                                                  
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: LITRI                                             NICKEL SULFATE HEXAHYDRATE                              DATE: 05/20/95       
EXPERIMENT: 05122 TEST: 06                                                                                     TIME: 04:06:44       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                        
CONT: Y01ES30108                                ROUTE: RESPIRATORY EXPOSURE WHOLE BODY                         NTP C#: C60344       
PATHOLOGIST: F. HAHN                                                                                           CAS: 010101970       


                                                    MICE(B6C3F1)
                                              ----------------------------
             FOR ALL DOSES THE TUMOR RATES IN THE FOLLOWING TISSUES/ORGANS ARE BASED ON NUMBER OF TISSUES EXAMINED.
             IN OTHER TISSUES/ORGANS, RATES ARE BASED ON NUMBER OF ANIMALS NECROPSIED.
                                                                    
                                                    ----------------
                                                    Adrenal Cortex
                                                    Adrenal Medulla
                                                    Bone Marrow
                                                    Brain
                                                    Clitoral Gland
                                                    Epididymis
                                                    Gallbladder
                                                    Heart
                                                    Islets, Pancreatic
                                                    Kidney
                                                    Larynx
                                                    Liver
                                                    Lung
                                                    Nose
                                                    Ovary
                                                    Pancreas
                                                    Parathyroid Gland
                                                    Pituitary Gland
                                                    Preputial Gland
                                                    Prostate
                                                    Salivary Glands
                                                    Spleen
                                                    Testes
                                                    Thymus
                                                    Thyroid Gland
                                                    Urinary Bladder
NTP                                        STATISTICAL ANALYSIS OF PRIMARY TUMORS                              REPORT: PEIRPT08     
LAB: LITRI                                             NICKEL SULFATE HEXAHYDRATE                              DATE: 05/20/95       
EXPERIMENT: 05122 TEST: 06                                                                                     TIME: 04:06:44       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                        
CONT: Y01ES30108                                ROUTE: RESPIRATORY EXPOSURE WHOLE BODY                         NTP C#: C60344       
PATHOLOGIST: F. HAHN                                                                                           CAS: 010101970       


                                     SUMMARY OF STATISTICALLY SIGNIFICANT (P<=.05) RESULTS
                                   IN THE ANALYSIS OF THE STUDY OF NICKEL SULFATE HEXAHYDRATE
              ====================================================================================================
                                                           MALE MICE
                                                          -------------
                  ORGAN                               MORPHOLOGY
                 -------                             ------------
              Liver                         Hepatocellular Adenoma
                                            Hepatocellular Carcinoma or Hepatocellular Adenoma
                                            Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma
              Lung                          Alveolar/Bronchiolar Carcinoma
                                            Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma
              All Organs                    Malignant Tumors
                                            Malignant and Benign Tumors
              ====================================================================================================
                                                           FEMALE MICE
                                                          -------------
                  ORGAN                               MORPHOLOGY
                 -------                             ------------
              Harderian Gland               Adenoma
              Liver                         Hepatocellular Carcinoma
                                            Hepatocellular Carcinoma or Hepatocellular Adenoma
                                            Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma
              Skin                          Fibrosarcoma
              All Organs                    Benign Tumors
              ====================================================================================================
         DATE: 05/20/95                     EXPERIMENT: 05122 TEST: 06                                                 PAGE   1
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- NICKEL SULFATE HEXAHYDRATE
                                                 MALE TERMINAL SACRIFICE AT 106 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |
|                       |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |
|=================================================================================================================================|
|                                                                                                                                 |
|Adrenal Cortex                                                                                                                   |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/66 (2%)    2/65 (3%)    3/66 (5%)    1/65 (2%)  |  1/65 (2%)    0/63 (0%)    0/65 (0%)    0/65 (0%)  |
|ADJUSTED (b)           |   4.8%         3.9%        11.6%         2.4%      |   2.3%         0.0%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/0          0/0          0/0          0/0        |  0/0          0/0          0/0          0/0        |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     1/5 (20%)    0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |  1/26 (4%)    0/22 (0%)    1/24 (4%)    0/25 (0%)  |  0/34 (0%)    0/39 (0%)    0/45 (0%)    0/37 (0%)  |
|FIRST INCIDENCE (DAYS) |   737 (T)      458 (I)      621          601       |   696          ---          ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.537N     P=0.466      P=0.304      P=0.749N   |  P=0.308N     P=0.500N     P=0.487N     P=0.509N   |
|INCIDENTAL TUMOR       |  P=0.599      P=0.453      P=0.311      P=0.716    |  P=0.347N     P=0.590N     P=0.590N     P=0.564N   |
|LOGISTIC REGRESSION    |  P=0.581N     P=0.512      P=0.299      P=0.762N   |  P=0.304N     P=0.500N     P=0.487N     P=0.503N   |
|COCHRAN-ARMITAGE       |  P=0.581N                                          |  P=0.305N                                          |
|FISHER EXACT           |               P=0.494      P=0.310      P=0.748    |               P=0.508N     P=0.500N     P=0.500N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Harderian Gland                                                                                                                  |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/71 (0%)    2/71 (3%)    2/72 (3%)    1/72 (1%)  |  0/71 (0%)    2/70 (3%)    4/70 (6%)    1/70 (1%)  |
|ADJUSTED (b)           |   0.0%         8.0%         6.0%         3.8%      |   0.0%         4.6%         8.3%         1.7%      |
|INTERIM SACRIFICE 1 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     1/5 (20%)  |
|TERMINAL (d)           |  0/26 (0%)    1/23 (4%)    1/24 (4%)    0/25 (0%)  |  0/34 (0%)    1/39 (3%)    2/45 (4%)    0/37 (0%)  |
|FIRST INCIDENCE (DAYS) |   ---          714          538          720       |   ---          635          672          459 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.464      P=0.222      P=0.225      P=0.492    |  P=0.431      P=0.262      P=0.097      P=0.500    |
|INCIDENTAL TUMOR       |  P=0.559      P=0.298      P=0.281      P=0.638    |  P=0.373      P=0.222      P=0.046 *    P=0.500    |
|LOGISTIC REGRESSION    |  P=0.482      P=0.226      P=0.237      P=0.505    |  P=0.416      P=0.240      P=0.072      P=0.489    |
|COCHRAN-ARMITAGE       |  P=0.475                                           |  P=0.419                                           |
|FISHER EXACT           |               P=0.248      P=0.252      P=0.503    |               P=0.245      P=0.058      P=0.496    |
|=================================================================================================================================|
         DATE: 05/20/95                     EXPERIMENT: 05122 TEST: 06                                                 PAGE   2
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- NICKEL SULFATE HEXAHYDRATE
                                                 MALE TERMINAL SACRIFICE AT 106 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |
|                       |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |
|=================================================================================================================================|
|                                                                                                                                 |
|Islets, Pancreatic                                                                                                               |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/66 (0%)    0/65 (0%)    1/62 (2%)    0/63 (0%)  |  2/65 (3%)    1/61 (2%)    1/64 (2%)    1/65 (2%)  |
|ADJUSTED (b)           |   0.0%         0.0%         2.9%         0.0%      |   5.0%         2.7%         2.2%         2.7%      |
|INTERIM SACRIFICE 1 (c)|  0/0          0/0          0/0          0/0        |  0/0          0/0          0/0          0/0        |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |  0/26 (0%)    0/22 (0%)    0/23 (0%)    0/25 (0%)  |  1/33 (3%)    1/37 (3%)    1/45 (2%)    1/37 (3%)  |
|FIRST INCIDENCE (DAYS) |   ---          ---          614          ---       |   626          730 (T)      730 (T)      730 (T)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.708        (e)        P=0.512        (e)      |  P=0.384N     P=0.476N     P=0.422N     P=0.486N   |
|INCIDENTAL TUMOR       |  P=0.646        (e)        P=0.451        (e)      |  P=0.397N     P=0.527N     P=0.506N     P=0.507N   |
|LOGISTIC REGRESSION    |  P=0.687        (e)        P=0.488        (e)      |  P=0.406N     P=0.512N     P=0.481N     P=0.503N   |
|COCHRAN-ARMITAGE       |  P=0.688                                           |  P=0.404N                                          |
|FISHER EXACT           |                 (e)        P=0.484        (e)      |               P=0.524N     P=0.506N     P=0.500N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hemangiosarcoma                                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/66 (2%)    0/67 (0%)    2/68 (3%)    0/65 (0%)  |  0/66 (0%)    0/64 (0%)    0/65 (0%)    1/65 (2%)  |
|ADJUSTED (b)           |   3.4%         0.0%         8.3%         0.0%      |   0.0%         0.0%         0.0%         2.3%      |
|INTERIM SACRIFICE 1 (c)|  0/0          0/1 (0%)     0/1 (0%)     0/0        |  0/0          0/0          0/0          0/0        |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |  0/26 (0%)    0/23 (0%)    2/24 (8%)    0/25 (0%)  |  0/34 (0%)    0/39 (0%)    0/45 (0%)    0/37 (0%)  |
|FIRST INCIDENCE (DAYS) |   687          ---          737 (T)      ---       |   ---          ---          ---          675       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.463N     P=0.500N     P=0.485      P=0.500N   |  P=0.194        (e)          (e)        P=0.500    |
|INCIDENTAL TUMOR       |  P=0.424N     P=0.362N     P=0.535      P=0.362N   |  P=0.169        (e)          (e)        P=0.419    |
|LOGISTIC REGRESSION    |  P=0.452N     P=0.507N     P=0.496      P=0.498N   |  P=0.197        (e)          (e)        P=0.496    |
|COCHRAN-ARMITAGE       |  P=0.465N                                          |  P=0.198                                           |
|FISHER EXACT           |               P=0.496N     P=0.511      P=0.504N   |                 (e)          (e)        P=0.496    |
|=================================================================================================================================|
         DATE: 05/20/95                     EXPERIMENT: 05122 TEST: 06                                                 PAGE   3
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- NICKEL SULFATE HEXAHYDRATE
                                                 MALE TERMINAL SACRIFICE AT 106 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |
|                       |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Adenoma                                                                                                      |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 20/66 (30%)   8/67 (12%)  19/68 (28%)  10/65 (15%) | 13/66 (20%)  14/64 (22%)  13/65 (20%)  10/65 (15%) |
|ADJUSTED (b)           |  48.8%        18.8%        49.6%        25.2%      |  35.0%        34.7%        26.3%        23.5%      |
|INTERIM SACRIFICE 1 (c)|  0/0          0/1 (0%)     0/1 (0%)     0/0        |  0/0          0/0          0/0          0/0        |
|INTERIM SACRIFICE 2 (c)|  2/5 (40%)    1/5 (20%)    1/5 (20%)    0/5 (0%)   |  0/5 (0%)     0/5 (0%)     2/5 (40%)    1/5 (20%)  |
|TERMINAL (d)           |  9/26 (35%)   0/23 (0%)    8/24 (33%)   3/25 (12%) | 11/34 (32%)  13/39 (33%)  10/45 (22%)   6/37 (16%) |
|FIRST INCIDENCE (DAYS) |   395          458 (I)      284          404       |   547          635          459 (I)      459 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.102N     P=0.024N*    P=0.559N     P=0.040N*  |  P=0.190N     P=0.529N     P=0.324N     P=0.269N   |
|INCIDENTAL TUMOR       |  P=0.118N     P=0.014N*    P=0.506N     P=0.055N   |  P=0.232N     P=0.577N     P=0.405N     P=0.339N   |
|LOGISTIC REGRESSION    |  P=0.103N     P=0.009N**   P=0.483N     P=0.034N*  |  P=0.260N     P=0.524      P=0.527N     P=0.349N   |
|COCHRAN-ARMITAGE       |  P=0.107N                                          |  P=0.252N                                          |
|FISHER EXACT           |               P=0.008N**   P=0.456N     P=0.033N*  |               P=0.464      P=0.569      P=0.338N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma                                                                                                    |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 11/66 (17%)  11/67 (16%)  15/68 (22%)  10/65 (15%) |  7/66 (11%)  14/64 (22%)   5/65 (8%)   11/65 (17%) |
|ADJUSTED (b)           |  24.3%        31.8%        39.7%        26.9%      |  17.4%        27.7%        10.8%        23.6%      |
|INTERIM SACRIFICE 1 (c)|  0/0          0/1 (0%)     0/1 (0%)     0/0        |  0/0          0/0          0/0          0/0        |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |  2/26 (8%)    3/23 (13%)   6/24 (25%)   3/25 (12%) |  4/34 (12%)   4/39 (10%)   4/45 (9%)    5/37 (14%) |
|FIRST INCIDENCE (DAYS) |   452          552          482          533       |   530          511          681          516       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.461N     P=0.499      P=0.235      P=0.478N   |  P=0.410      P=0.123      P=0.245N     P=0.251    |
|INCIDENTAL TUMOR       |  P=0.468N     P=0.565N     P=0.224      P=0.404N   |  P=0.325      P=0.032 *    P=0.358N     P=0.213    |
|LOGISTIC REGRESSION    |  P=0.528N     P=0.592      P=0.201      P=0.570N   |  P=0.324      P=0.039 *    P=0.338N     P=0.171    |
|COCHRAN-ARMITAGE       |  P=0.508N                                          |  P=0.387                                           |
|FISHER EXACT           |               P=0.577N     P=0.285      P=0.515N   |               P=0.065      P=0.392N     P=0.213    |
|=================================================================================================================================|
         DATE: 05/20/95                     EXPERIMENT: 05122 TEST: 06                                                 PAGE   4
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- NICKEL SULFATE HEXAHYDRATE
                                                 MALE TERMINAL SACRIFICE AT 106 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |
|                       |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma or Hepatocellular Adenoma                                                                          |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 29/66 (44%)  19/67 (28%)  30/68 (44%)  19/65 (29%) | 18/66 (27%)  28/64 (44%)  17/65 (26%)  20/65 (31%) |
|ADJUSTED (b)           |  61.5%        44.6%        67.8%        44.6%      |  45.9%        55.5%        34.7%        42.0%      |
|INTERIM SACRIFICE 1 (c)|  0/0          0/1 (0%)     0/1 (0%)     0/0        |  0/0          0/0          0/0          0/0        |
|INTERIM SACRIFICE 2 (c)|  2/5 (40%)    1/5 (20%)    1/5 (20%)    0/5 (0%)   |  0/5 (0%)     0/5 (0%)     2/5 (40%)    1/5 (20%)  |
|TERMINAL (d)           | 11/26 (42%)   3/23 (13%)  12/24 (50%)   6/25 (24%) | 14/34 (41%)  17/39 (44%)  14/45 (31%)  11/37 (30%) |
|FIRST INCIDENCE (DAYS) |   395          458 (I)      284          404       |   530          511          459 (I)      459 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.134N     P=0.138N     P=0.424      P=0.076N   |  P=0.364N     P=0.125      P=0.204N     P=0.493    |
|INCIDENTAL TUMOR       |  P=0.123N     P=0.050N     P=0.482      P=0.056N   |  P=0.447N     P=0.041 *    P=0.296N     P=0.425    |
|LOGISTIC REGRESSION    |  P=0.133N     P=0.052N     P=0.534      P=0.058N   |  P=0.446N     P=0.031 *    P=0.420N     P=0.387    |
|COCHRAN-ARMITAGE       |  P=0.133N                                          |  P=0.431N                                          |
|FISHER EXACT           |               P=0.045N*    P=0.561      P=0.058N   |               P=0.037 *    P=0.521N     P=0.402    |
|=================================================================================================================================|
|                                                                                                                                 |
|Liver                                                                                                                            |
|     Hepatocellular Carcinoma, Hepatocellular Adenoma, or Hepatoblastoma                                                         |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 29/66 (44%)  19/67 (28%)  30/68 (44%)  19/65 (29%) | 18/66 (27%)  28/64 (44%)  17/65 (26%)  20/65 (31%) |
|ADJUSTED (b)           |  61.5%        44.6%        67.8%        44.6%      |  45.9%        55.5%        34.7%        42.0%      |
|INTERIM SACRIFICE 1 (c)|  0/0          0/1 (0%)     0/1 (0%)     0/0        |  0/0          0/0          0/0          0/0        |
|INTERIM SACRIFICE 2 (c)|  2/5 (40%)    1/5 (20%)    1/5 (20%)    0/5 (0%)   |  0/5 (0%)     0/5 (0%)     2/5 (40%)    1/5 (20%)  |
|TERMINAL (d)           | 11/26 (42%)   3/23 (13%)  12/24 (50%)   6/25 (24%) | 14/34 (41%)  17/39 (44%)  14/45 (31%)  11/37 (30%) |
|FIRST INCIDENCE (DAYS) |   395          458 (I)      284          404       |   530          511          459 (I)      459 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.134N     P=0.138N     P=0.424      P=0.076N   |  P=0.364N     P=0.125      P=0.204N     P=0.493    |
|INCIDENTAL TUMOR       |  P=0.123N     P=0.050N     P=0.482      P=0.056N   |  P=0.447N     P=0.041 *    P=0.296N     P=0.425    |
|LOGISTIC REGRESSION    |  P=0.133N     P=0.052N     P=0.534      P=0.058N   |  P=0.446N     P=0.031 *    P=0.420N     P=0.387    |
|COCHRAN-ARMITAGE       |  P=0.133N                                          |  P=0.431N                                          |
|FISHER EXACT           |               P=0.045N*    P=0.561      P=0.058N   |               P=0.037 *    P=0.521N     P=0.402    |
|=================================================================================================================================|
         DATE: 05/20/95                     EXPERIMENT: 05122 TEST: 06                                                 PAGE   5
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- NICKEL SULFATE HEXAHYDRATE
                                                 MALE TERMINAL SACRIFICE AT 106 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |
|                       |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Adenoma                                                                                                |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  5/71 (7%)    5/71 (7%)    3/72 (4%)    5/71 (7%)  |  3/71 (4%)    3/70 (4%)    2/70 (3%)    0/70 (0%)  |
|ADJUSTED (b)           |  18.4%        17.2%         9.1%        17.2%      |   8.8%         7.2%         4.4%         0.0%      |
|INTERIM SACRIFICE 1 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |  4/26 (15%)   3/23 (13%)   1/24 (4%)    3/25 (12%) |  3/34 (9%)    1/39 (3%)    2/45 (4%)    0/37 (0%)  |
|FIRST INCIDENCE (DAYS) |   688          552          552          597       |   730 (T)      711          730 (T)      ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.525N     P=0.574      P=0.384N     P=0.615    |  P=0.059N     P=0.602N     P=0.373N     P=0.106N   |
|INCIDENTAL TUMOR       |  P=0.525N     P=0.588      P=0.337N     P=0.583N   |  P=0.076N     P=0.661      P=0.373N     P=0.106N   |
|LOGISTIC REGRESSION    |  P=0.508N     P=0.606      P=0.363N     P=0.614N   |  P=0.067N     P=0.637N     P=0.373N     P=0.106N   |
|COCHRAN-ARMITAGE       |  P=0.532N                                          |  P=0.075N                                          |
|FISHER EXACT           |               P=0.628N     P=0.352N     P=0.628N   |               P=0.653      P=0.507N     P=0.125N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Carcinoma                                                                                              |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  9/71 (13%)  13/71 (18%)   4/72 (6%)    3/71 (4%)  |  4/71 (6%)    3/70 (4%)    9/70 (13%)   2/70 (3%)  |
|ADJUSTED (b)           |  29.5%        51.6%        13.0%         9.8%      |  10.7%         7.1%        18.9%         4.3%      |
|INTERIM SACRIFICE 1 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |  6/26 (23%)  11/23 (48%)   2/24 (8%)    2/25 (8%)  |  3/34 (9%)    2/39 (5%)    7/45 (16%)   0/37 (0%)  |
|FIRST INCIDENCE (DAYS) |   516          687          613          561       |   627          649          656          562       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.012N*    P=0.155      P=0.138N     P=0.068N   |  P=0.378N     P=0.444N     P=0.238      P=0.332N   |
|INCIDENTAL TUMOR       |  P=0.009N**   P=0.250      P=0.111N     P=0.044N*  |  P=0.452N     P=0.491N     P=0.151      P=0.394N   |
|LOGISTIC REGRESSION    |  P=0.009N**   P=0.191      P=0.122N     P=0.057N   |  P=0.417N     P=0.489N     P=0.155      P=0.349N   |
|COCHRAN-ARMITAGE       |  P=0.013N*                                         |  P=0.410N                                          |
|FISHER EXACT           |               P=0.244      P=0.117N     P=0.064N   |               P=0.508N     P=0.116      P=0.347N   |
|=================================================================================================================================|
         DATE: 05/20/95                     EXPERIMENT: 05122 TEST: 06                                                 PAGE   6
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- NICKEL SULFATE HEXAHYDRATE
                                                 MALE TERMINAL SACRIFICE AT 106 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |
|                       |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |
|=================================================================================================================================|
|                                                                                                                                 |
|Lung                                                                                                                             |
|     Alveolar/Bronchiolar Carcinoma or Alveolar/Bronchiolar Adenoma                                                              |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 13/71 (18%)  18/71 (25%)   7/72 (10%)   8/71 (11%) |  7/71 (10%)   6/70 (9%)   10/70 (14%)   2/70 (3%)  |
|ADJUSTED (b)           |  43.6%        65.4%        21.2%        26.2%      |  19.3%        13.9%        21.1%         4.3%      |
|INTERIM SACRIFICE 1 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           | 10/26 (38%)  14/23 (61%)   3/24 (13%)   5/25 (20%) |  6/34 (18%)   3/39 (8%)    8/45 (18%)   0/37 (0%)  |
|FIRST INCIDENCE (DAYS) |   516          552          552          561       |   627          649          656          562       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.040N*    P=0.105      P=0.135N     P=0.179N   |  P=0.089N     P=0.415N     P=0.512      P=0.077N   |
|INCIDENTAL TUMOR       |  P=0.033N*    P=0.157      P=0.103N     P=0.126N   |  P=0.131N     P=0.495N     P=0.404      P=0.096N   |
|LOGISTIC REGRESSION    |  P=0.028N*    P=0.144      P=0.110N     P=0.141N   |  P=0.111N     P=0.477N     P=0.377      P=0.089N   |
|COCHRAN-ARMITAGE       |  P=0.045N*                                         |  P=0.112N                                          |
|FISHER EXACT           |               P=0.208      P=0.107N     P=0.172N   |               P=0.511N     P=0.292      P=0.086N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Mammary Gland                                                                                                                    |
|     Carcinoma                                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/71 (0%)    0/71 (0%)    0/72 (0%)    0/72 (0%)  |  2/71 (3%)    1/70 (1%)    0/70 (0%)    1/70 (1%)  |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%         0.0%      |   5.9%         2.3%         0.0%         2.7%      |
|INTERIM SACRIFICE 1 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |  0/26 (0%)    0/23 (0%)    0/24 (0%)    0/25 (0%)  |  2/34 (6%)    0/39 (0%)    0/45 (0%)    1/37 (3%)  |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          ---       |   730 (T)      687          ---          730 (T)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |    (e)          (e)          (e)          (e)      |  P=0.352N     P=0.466N     P=0.179N     P=0.470N   |
|INCIDENTAL TUMOR       |    (e)          (e)          (e)          (e)      |  P=0.379N     P=0.501N     P=0.179N     P=0.470N   |
|LOGISTIC REGRESSION    |    (e)          (e)          (e)          (e)      |  P=0.371N     P=0.489N     P=0.179N     P=0.470N   |
|COCHRAN-ARMITAGE       |    (e)                                             |  P=0.370N                                          |
|FISHER EXACT           |                 (e)          (e)          (e)      |               P=0.505N     P=0.252N     P=0.505N   |
|=================================================================================================================================|
         DATE: 05/20/95                     EXPERIMENT: 05122 TEST: 06                                                 PAGE   7
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- NICKEL SULFATE HEXAHYDRATE
                                                 MALE TERMINAL SACRIFICE AT 106 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |
|                       |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |
|=================================================================================================================================|
|                                                                                                                                 |
|Mammary Gland                                                                                                                    |
|     Fibroma, Fibroadenoma, Carcinoma, or Adenoma                                                                                |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/71 (0%)    0/71 (0%)    0/72 (0%)    0/72 (0%)  |  2/71 (3%)    2/70 (3%)    0/70 (0%)    1/70 (1%)  |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%         0.0%      |   5.9%         4.8%         0.0%         2.7%      |
|INTERIM SACRIFICE 1 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |  0/26 (0%)    0/23 (0%)    0/24 (0%)    0/25 (0%)  |  2/34 (6%)    1/39 (3%)    0/45 (0%)    1/37 (3%)  |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          ---       |   730 (T)      687          ---          730 (T)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |    (e)          (e)          (e)          (e)      |  P=0.278N     P=0.654N     P=0.179N     P=0.470N   |
|INCIDENTAL TUMOR       |    (e)          (e)          (e)          (e)      |  P=0.300N     P=0.683N     P=0.179N     P=0.470N   |
|LOGISTIC REGRESSION    |    (e)          (e)          (e)          (e)      |  P=0.299N     P=0.682N     P=0.179N     P=0.470N   |
|COCHRAN-ARMITAGE       |    (e)                                             |  P=0.300N                                          |
|FISHER EXACT           |                 (e)          (e)          (e)      |               P=0.685      P=0.252N     P=0.505N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Ovary                                                                                                                            |
|     Cystadenoma                                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |  2/64 (3%)    1/63 (2%)    0/65 (0%)    0/64 (0%)  |
|ADJUSTED (b)           |                                                    |   5.8%         2.6%         0.0%         0.0%      |
|INTERIM SACRIFICE 1 (c)|                                                    |  0/0          0/0          0/0          0/0        |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |                                                    |  1/33 (3%)    1/38 (3%)    0/45 (0%)    0/37 (0%)  |
|FIRST INCIDENCE (DAYS) |                                                    |   728          730 (T)      ---          ---       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |  P=0.085N     P=0.452N     P=0.181N     P=0.218N   |
|INCIDENTAL TUMOR       |                                                    |  P=0.106N     P=0.514N     P=0.259N     P=0.270N   |
|LOGISTIC REGRESSION    |                                                    |  P=0.089N     P=0.466N     P=0.193N     P=0.225N   |
|COCHRAN-ARMITAGE       |                                                    |  P=0.100N                                          |
|FISHER EXACT           |                                                    |               P=0.506N     P=0.244N     P=0.248N   |
|=================================================================================================================================|
         DATE: 05/20/95                     EXPERIMENT: 05122 TEST: 06                                                 PAGE   8
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- NICKEL SULFATE HEXAHYDRATE
                                                 MALE TERMINAL SACRIFICE AT 106 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |
|                       |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |
|=================================================================================================================================|
|                                                                                                                                 |
|Pituitary Gland: Pars Distalis or Unspecified Site                                                                               |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/58 (0%)    0/57 (0%)    0/63 (0%)    0/62 (0%)  |  8/64 (13%)   9/61 (15%)   5/64 (8%)    4/61 (7%)  |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%         0.0%      |  20.6%        21.8%        11.1%         9.4%      |
|INTERIM SACRIFICE 1 (c)|  0/0          0/0          0/0          0/0        |  0/0          0/0          0/0          0/0        |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/3 (0%)     0/5 (0%)     0/4 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/4 (0%)   |
|TERMINAL (d)           |  0/25 (0%)    0/22 (0%)    0/24 (0%)    0/24 (0%)  |  4/33 (12%)   7/38 (18%)   5/45 (11%)   1/37 (3%)  |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          ---       |   696          559          730 (T)      601       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |    (e)          (e)          (e)          (e)      |  P=0.072N     P=0.589      P=0.144N     P=0.172N   |
|INCIDENTAL TUMOR       |    (e)          (e)          (e)          (e)      |  P=0.108N     P=0.425      P=0.241N     P=0.271N   |
|LOGISTIC REGRESSION    |    (e)          (e)          (e)          (e)      |  P=0.086N     P=0.497      P=0.189N     P=0.186N   |
|COCHRAN-ARMITAGE       |    (e)                                             |  P=0.102N                                          |
|FISHER EXACT           |                 (e)          (e)          (e)      |               P=0.457      P=0.280N     P=0.206N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Skin                                                                                                                             |
|     Fibrosarcoma                                                                                                                |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/71 (0%)    0/71 (0%)    0/72 (0%)    0/72 (0%)  |  5/71 (7%)    0/70 (0%)    1/70 (1%)    2/70 (3%)  |
|ADJUSTED (b)           |   0.0%         0.0%         0.0%         0.0%      |   9.3%         0.0%         1.7%         4.3%      |
|INTERIM SACRIFICE 1 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |  0/26 (0%)    0/23 (0%)    0/24 (0%)    0/25 (0%)  |  0/34 (0%)    0/39 (0%)    0/45 (0%)    1/37 (3%)  |
|FIRST INCIDENCE (DAYS) |   ---          ---          ---          ---       |   384          ---          510          393       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |    (e)          (e)          (e)          (e)      |  P=0.239N     P=0.037N*    P=0.096N     P=0.233N   |
|INCIDENTAL TUMOR       |    (e)          (e)          (e)          (e)      |  P=0.168N     P=0.033N*    P=0.173N     P=0.143N   |
|LOGISTIC REGRESSION    |    (e)          (e)          (e)          (e)      |  P=0.202N     P=0.037N*    P=0.130N     P=0.226N   |
|COCHRAN-ARMITAGE       |    (e)                                             |  P=0.237N                                          |
|FISHER EXACT           |                 (e)          (e)          (e)      |               P=0.030N*    P=0.108N     P=0.226N   |
|=================================================================================================================================|
         DATE: 05/20/95                     EXPERIMENT: 05122 TEST: 06                                                 PAGE   9
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- NICKEL SULFATE HEXAHYDRATE
                                                 MALE TERMINAL SACRIFICE AT 106 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |
|                       |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |
|=================================================================================================================================|
|                                                                                                                                 |
|Thyroid Gland: Follicular Cell                                                                                                   |
|     Adenoma                                                                                                                     |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |                                                    |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  0/66 (0%)    2/66 (3%)    1/66 (2%)    1/67 (1%)  |  1/65 (2%)    1/64 (2%)    2/65 (3%)    1/65 (2%)  |
|ADJUSTED (b)           |   0.0%         8.3%         3.0%         4.8%      |   2.9%         2.6%         4.4%         2.7%      |
|INTERIM SACRIFICE 1 (c)|  0/0          0/0          0/0          0/0        |  0/0          0/0          0/0          0/0        |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |  0/26 (0%)    1/23 (4%)    0/24 (0%)    1/25 (4%)  |  1/34 (3%)    1/39 (3%)    2/45 (4%)    1/37 (3%)  |
|FIRST INCIDENCE (DAYS) |   ---          594          627          737 (T)   |   730 (T)      730 (T)      730 (T)      730 (T)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.530      P=0.209      P=0.519      P=0.500    |  P=0.617      P=0.731N     P=0.597      P=0.743N   |
|INCIDENTAL TUMOR       |  P=0.460      P=0.200      P=0.451      P=0.492    |  P=0.617      P=0.731N     P=0.597      P=0.743N   |
|LOGISTIC REGRESSION    |  P=0.510      P=0.231      P=0.500      P=0.492    |  P=0.617      P=0.731N     P=0.597      P=0.743N   |
|COCHRAN-ARMITAGE       |  P=0.505                                           |  P=0.592                                           |
|FISHER EXACT           |               P=0.248      P=0.500      P=0.504    |               P=0.748      P=0.500      P=0.752N   |
|=================================================================================================================================|
|                                                                                                                                 |
|Uterus                                                                                                                           |
|     Polyp Stromal                                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |                                                    |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |                                                    |  2/71 (3%)    3/70 (4%)    0/70 (0%)    1/70 (1%)  |
|ADJUSTED (b)           |                                                    |   5.9%         6.5%         0.0%         2.7%      |
|INTERIM SACRIFICE 1 (c)|                                                    |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|                                                    |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |                                                    |  2/34 (6%)    1/39 (3%)    0/45 (0%)    1/37 (3%)  |
|FIRST INCIDENCE (DAYS) |                                                    |   730 (T)      614          ---          730 (T)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |                                                    |  P=0.229N     P=0.545      P=0.179N     P=0.470N   |
|INCIDENTAL TUMOR       |                                                    |  P=0.263N     P=0.485      P=0.179N     P=0.470N   |
|LOGISTIC REGRESSION    |                                                    |  P=0.247N     P=0.507      P=0.179N     P=0.470N   |
|COCHRAN-ARMITAGE       |                                                    |  P=0.246N                                          |
|FISHER EXACT           |                                                    |               P=0.493      P=0.252N     P=0.505N   |
|=================================================================================================================================|
         DATE: 05/20/95                     EXPERIMENT: 05122 TEST: 06                                                 PAGE  10
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- NICKEL SULFATE HEXAHYDRATE
                                                 MALE TERMINAL SACRIFICE AT 106 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |
|                       |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangiosarcoma                                                                                                             |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  2/71 (3%)    1/71 (1%)    2/72 (3%)    0/72 (0%)  |  1/71 (1%)    0/70 (0%)    0/70 (0%)    1/70 (1%)  |
|ADJUSTED (b)           |   6.9%         4.3%         8.3%         0.0%      |   2.9%         0.0%         0.0%         2.3%      |
|INTERIM SACRIFICE 1 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |  0/26 (0%)    1/23 (4%)    2/24 (8%)    0/25 (0%)  |  1/34 (3%)    0/39 (0%)    0/45 (0%)    0/37 (0%)  |
|FIRST INCIDENCE (DAYS) |   687          737 (T)      737 (T)      ---       |   730 (T)      ---          ---          675       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.202N     P=0.514N     P=0.687      P=0.236N   |  P=0.604      P=0.473N     P=0.444N     P=0.751N   |
|INCIDENTAL TUMOR       |  P=0.162N     P=0.355N     P=0.626N     P=0.097N   |  P=0.560      P=0.473N     P=0.444N     P=0.726    |
|LOGISTIC REGRESSION    |  P=0.192N     P=0.514N     P=0.692      P=0.232N   |  P=0.591      P=0.473N     P=0.444N     P=0.756    |
|COCHRAN-ARMITAGE       |  P=0.197N                                          |  P=0.592                                           |
|FISHER EXACT           |               P=0.500N     P=0.685N     P=0.245N   |               P=0.504N     P=0.504N     P=0.748    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Hemangiosarcoma or Hemangioma                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  2/71 (3%)    1/71 (1%)    2/72 (3%)    0/72 (0%)  |  1/71 (1%)    1/70 (1%)    1/70 (1%)    2/70 (3%)  |
|ADJUSTED (b)           |   6.9%         4.3%         8.3%         0.0%      |   2.9%         2.6%         2.2%         5.0%      |
|INTERIM SACRIFICE 1 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |  0/26 (0%)    1/23 (4%)    2/24 (8%)    0/25 (0%)  |  1/34 (3%)    1/39 (3%)    1/45 (2%)    1/37 (3%)  |
|FIRST INCIDENCE (DAYS) |   687          737 (T)      737 (T)      ---       |   730 (T)      730 (T)      730 (T)      675       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.202N     P=0.514N     P=0.687      P=0.236N   |  P=0.364      P=0.731N     P=0.698N     P=0.520    |
|INCIDENTAL TUMOR       |  P=0.162N     P=0.355N     P=0.626N     P=0.097N   |  P=0.340      P=0.731N     P=0.698N     P=0.473    |
|LOGISTIC REGRESSION    |  P=0.192N     P=0.514N     P=0.692      P=0.232N   |  P=0.340      P=0.732N     P=0.698N     P=0.490    |
|COCHRAN-ARMITAGE       |  P=0.197N                                          |  P=0.348                                           |
|FISHER EXACT           |               P=0.500N     P=0.685N     P=0.245N   |               P=0.748      P=0.748      P=0.495    |
|=================================================================================================================================|
         DATE: 05/20/95                     EXPERIMENT: 05122 TEST: 06                                                 PAGE  11
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- NICKEL SULFATE HEXAHYDRATE
                                                 MALE TERMINAL SACRIFICE AT 106 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |
|                       |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Histiocytic Sarcoma                                                                                                         |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  1/71 (1%)    1/71 (1%)    2/72 (3%)    0/72 (0%)  |  1/71 (1%)    4/70 (6%)    2/70 (3%)    4/70 (6%)  |
|ADJUSTED (b)           |   3.8%         2.7%         5.9%         0.0%      |   2.3%         9.0%         4.0%         8.8%      |
|INTERIM SACRIFICE 1 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |  1/26 (4%)    0/23 (0%)    1/24 (4%)    0/25 (0%)  |  0/34 (0%)    2/39 (5%)    1/45 (2%)    1/37 (3%)  |
|FIRST INCIDENCE (DAYS) |   737 (T)      621          485          ---       |   670          398          577          571       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.360N     P=0.753      P=0.475      P=0.508N   |  P=0.217      P=0.213      P=0.551      P=0.181    |
|INCIDENTAL TUMOR       |  P=0.356N     P=0.719      P=0.535      P=0.508N   |  P=0.176      P=0.164      P=0.392      P=0.110    |
|LOGISTIC REGRESSION    |  P=0.360N     P=0.754      P=0.497      P=0.508N   |  P=0.212      P=0.180      P=0.506      P=0.089    |
|COCHRAN-ARMITAGE       |  P=0.362N                                          |  P=0.214                                           |
|FISHER EXACT           |               P=0.752N     P=0.505      P=0.497N   |               P=0.179      P=0.495      P=0.179    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Lymphoma And Histiocytic Sarcoma                                                                                  |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  3/71 (4%)    2/71 (3%)    5/72 (7%)    0/72 (0%)  |  8/71 (11%)  15/70 (21%)  11/70 (16%)  10/70 (14%) |
|ADJUSTED (b)           |  11.5%         6.1%        17.6%         0.0%      |  19.5%        33.6%        21.8%        22.7%      |
|INTERIM SACRIFICE 1 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |  3/26 (12%)   0/23 (0%)    3/24 (13%)   0/25 (0%)  |  3/34 (9%)   10/39 (26%)   7/45 (16%)   5/37 (14%) |
|FIRST INCIDENCE (DAYS) |   737 (T)      621          485          ---       |   626          398          567          571       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.165N     P=0.528N     P=0.315      P=0.126N   |  P=0.514N     P=0.146      P=0.495      P=0.429    |
|INCIDENTAL TUMOR       |  P=0.136N     P=0.495N     P=0.386      P=0.126N   |  P=0.435      P=0.065      P=0.199      P=0.261    |
|LOGISTIC REGRESSION    |  P=0.152N     P=0.519N     P=0.349      P=0.126N   |  P=0.518      P=0.088      P=0.360      P=0.374    |
|COCHRAN-ARMITAGE       |  P=0.159N                                          |  P=0.532                                           |
|FISHER EXACT           |               P=0.500N     P=0.367      P=0.120N   |               P=0.080      P=0.300      P=0.388    |
|=================================================================================================================================|
         DATE: 05/20/95                     EXPERIMENT: 05122 TEST: 06                                                 PAGE  12
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- NICKEL SULFATE HEXAHYDRATE
                                                 MALE TERMINAL SACRIFICE AT 106 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |
|                       |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Lymphoma: Histiocytic, Lymphocytic, Mixed, NOS, or Undifferentiated                                               |
|     Cell Type                                                                                                                   |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            |  2/71 (3%)    1/71 (1%)    3/72 (4%)    0/72 (0%)  |  7/71 (10%)  12/70 (17%)   9/70 (13%)   6/70 (9%)  |
|ADJUSTED (b)           |   7.7%         3.4%        12.0%         0.0%      |  17.6%        28.4%        18.3%        15.0%      |
|INTERIM SACRIFICE 1 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |  2/26 (8%)    0/23 (0%)    2/24 (8%)    0/25 (0%)  |  3/34 (9%)    9/39 (23%)   6/45 (13%)   4/37 (11%) |
|FIRST INCIDENCE (DAYS) |   737 (T)      687          728          ---       |   626          687          567          596       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.248N     P=0.528N     P=0.464      P=0.246N   |  P=0.258N     P=0.238      P=0.574      P=0.467N   |
|INCIDENTAL TUMOR       |  P=0.203N     P=0.450N     P=0.513      P=0.246N   |  P=0.357N     P=0.144      P=0.310      P=0.592N   |
|LOGISTIC REGRESSION    |  P=0.233N     P=0.516N     P=0.480      P=0.244N   |  P=0.305N     P=0.175      P=0.453      P=0.523N   |
|COCHRAN-ARMITAGE       |  P=0.238N                                          |  P=0.298N                                          |
|FISHER EXACT           |               P=0.500N     P=0.507      P=0.245N   |               P=0.154      P=0.384      P=0.511N   |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Benign Tumors                                                                                                               |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 24/71 (34%)  18/71 (25%)  25/72 (35%)  16/72 (22%) | 27/71 (38%)  35/70 (50%)  24/70 (34%)  18/70 (26%) |
|ADJUSTED (b)           |  59.4%        43.8%        60.0%        42.4%      |  60.9%        71.0%        47.4%        39.8%      |
|INTERIM SACRIFICE 1 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|  2/5 (40%)    2/5 (40%)    1/5 (20%)    0/5 (0%)   |  0/5 (0%)     1/5 (20%)    2/5 (40%)    2/5 (40%)  |
|TERMINAL (d)           | 12/26 (46%)   5/23 (22%)  10/24 (42%)   7/25 (28%) | 17/34 (50%)  25/39 (64%)  19/45 (42%)  11/37 (30%) |
|FIRST INCIDENCE (DAYS) |   395          458 (I)      284          404       |   547          459 (I)      459 (I)      459 (I)   |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.142N     P=0.280N     P=0.441      P=0.112N   |  P=0.009N**   P=0.278      P=0.088N     P=0.059N   |
|INCIDENTAL TUMOR       |  P=0.139N     P=0.220N     P=0.487      P=0.108N   |  P=0.018N*    P=0.095      P=0.255N     P=0.108N   |
|LOGISTIC REGRESSION    |  P=0.114N     P=0.201N     P=0.487      P=0.079N   |  P=0.014N*    P=0.109      P=0.234N     P=0.079N   |
|COCHRAN-ARMITAGE       |  P=0.127N                                          |  P=0.017N*                                         |
|FISHER EXACT           |               P=0.179N     P=0.524      P=0.087N   |               P=0.103      P=0.387N     P=0.082N   |
|=================================================================================================================================|
         DATE: 05/20/95                     EXPERIMENT: 05122 TEST: 06                                                 PAGE  13
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- NICKEL SULFATE HEXAHYDRATE
                                                 MALE TERMINAL SACRIFICE AT 106 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 105 WEEKS
|=================================================================================================================================|
|                       |                                                    |                                                    |
|                       |                       MALES                        |                      FEMALES                       |
|                       |                                                    |                                                    |
|DOSE                   |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |   0.0MG/M3     .25MG/M3     0.5MG/M3     1.0MG/M3  |
|                       |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |   LUNG TOX     LUNG TOX     LUNG TOX     LUNG TOX  |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant Tumors                                                                                                            |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 20/71 (28%)  22/71 (31%)  25/72 (35%)  13/72 (18%) | 26/71 (37%)  27/70 (39%)  26/70 (37%)  26/70 (37%) |
|ADJUSTED (b)           |  48.8%        66.8%        64.1%        34.8%      |  53.0%        52.3%        47.7%        50.0%      |
|INTERIM SACRIFICE 1 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|TERMINAL (d)           |  8/26 (31%)  13/23 (57%)  12/24 (50%)   5/25 (20%) | 12/34 (35%)  15/39 (38%)  17/45 (38%)  12/37 (32%) |
|FIRST INCIDENCE (DAYS) |   452          552          482          533       |   384          398          510          393       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.095N     P=0.302      P=0.205      P=0.129N   |  P=0.467N     P=0.478N     P=0.252N     P=0.516N   |
|INCIDENTAL TUMOR       |  P=0.057N     P=0.439      P=0.227      P=0.044N*  |  P=0.458      P=0.413      P=0.436      P=0.470    |
|LOGISTIC REGRESSION    |  P=0.064N     P=0.365      P=0.213      P=0.101N   |  P=0.499      P=0.512      P=0.491N     P=0.518    |
|COCHRAN-ARMITAGE       |  P=0.081N                                          |  P=0.530N                                          |
|FISHER EXACT           |               P=0.427      P=0.254      P=0.108N   |               P=0.474      P=0.544      P=0.544    |
|=================================================================================================================================|
|                                                                                                                                 |
|All Organs                                                                                                                       |
|     Malignant and Benign Tumors                                                                                                 |
|                                                                                                                                 |
|---------------------------------------------------------------------------------------------------------------------------------|
|TUMOR RATES            |    #            #            #            #        |    #            #            #            #        |
|-----------            |                                                    |                                                    |
|OVERALL (a)            | 40/71 (56%)  38/71 (54%)  42/72 (58%)  27/72 (38%) | 41/71 (58%)  46/70 (66%)  42/70 (60%)  38/70 (54%) |
|ADJUSTED (b)           |  83.8%        85.0%        82.6%        62.3%      |  80.0%        80.6%        72.2%        67.6%      |
|INTERIM SACRIFICE 1 (c)|  0/5 (0%)     1/5 (20%)    0/5 (0%)     0/5 (0%)   |  0/5 (0%)     0/5 (0%)     0/5 (0%)     0/5 (0%)   |
|INTERIM SACRIFICE 2 (c)|  2/5 (40%)    2/5 (40%)    1/5 (20%)    0/5 (0%)   |  0/5 (0%)     1/5 (20%)    2/5 (40%)    2/5 (40%)  |
|TERMINAL (d)           | 19/26 (73%)  17/23 (74%)  16/24 (67%)  11/25 (44%) | 24/34 (71%)  28/39 (72%)  29/45 (64%)  19/37 (51%) |
|FIRST INCIDENCE (DAYS) |   395          206 (I)      284          404       |   384          398          459 (I)      393       |
|-----------------------|                                                    |                                                    |
|STATISTICAL TESTS (f)  |                                                    |                                                    |
|-----------------------|                                                    |                                                    |
|LIFE TABLE             |  P=0.033N*    P=0.485      P=0.363      P=0.042N*  |  P=0.203N     P=0.511      P=0.157N     P=0.318N   |
|INCIDENTAL TUMOR       |  P=0.009N**   P=0.489N     P=0.429      P=0.008N** |  P=0.304N     P=0.170      P=0.510      P=0.474N   |
|LOGISTIC REGRESSION    |  P=0.008N**   P=0.519N     P=0.399      P=0.013N*  |  P=0.260N     P=0.230      P=0.502N     P=0.430N   |
|COCHRAN-ARMITAGE       |  P=0.014N*                                         |  P=0.250N                                          |
|FISHER EXACT           |               P=0.433N     P=0.471      P=0.018N*  |               P=0.212      P=0.460      P=0.404N   |
|=================================================================================================================================|
         DATE: 05/20/95                     EXPERIMENT: 05122 TEST: 06                                                 PAGE  14
                         STATISTICAL ANALYSIS OF PRIMARY TUMORS IN MICE(B6C3F1) -- NICKEL SULFATE HEXAHYDRATE
                                                 MALE TERMINAL SACRIFICE AT 106 WEEKS
                                                 FEMALE TERMINAL SACRIFICE AT 105 WEEKS
                                                                           
 (a)  Number of tumor-bearing animals / number of animals examined at site.
 (b)  Kaplan-Meier estimated lifetime tumor incidence after adjustment for 
      intercurrent mortality.
 (c)  Observed incidence at interim kill.
          Male interim sacrifices(days):   206  458
          Female interim sacrifices(days): 207  459
 (d)  Observed incidence at terminal kill.
 (f)  Beneath the control incidence are the P-values associated with the trend 
      test.  Beneath the dosed group incidence are the P-values corresponding to 
      pairwise comparisons between the controls and that dosed group.  The life
      table analysis regards tumors in animals dying  prior to terminal kill as
      being (directly or indirectly) the cause of death.  The incidental tumor 
      test regards these lesions as non-fatal.  Logistic regression is an alternative
      method for analyzing the incidence of non-fatal tumors.  The Cochran-Armitage
      and Fisher's exact tests compare directly the overall incidence rates.  
      For all tests, a negative trend is indicated by "N".
 (e)  Value of Statistic cannot be computed. 
 (I)  Interim sacrifice
 (T)  Terminal sacrifice
  #   Tumor rates based on number of animals necropsied.
  *   To the right of any statistical result, indicates significance at (P<=0.05).
  **  To the right of any statistical result, indicates significance at (P<=0.01).