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TDMS Study 05130-02 Pathology Tables



                              INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)
                                                       C. I. DIRECT BLUE 218


NTP Experiment-Test: 05130-02                                                                                     Report: PEIRPT05
Study Type: CHRONIC                                                                                               Date: 09/16/94
Route: DOSED FEED                                                                                                 Time: 23:49:54




       Facility:  Microbiological Associates

       Chemical CAS #:  10401-500

       Lock Date:  10/29/91

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All
































a  Number of animals examined microscopically at site and number of animals with lesion

                                                             Page   1

NTP Experiment-Test: 05130-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/16/94  
Route: DOSED FEED                                                                                                 Time: 23:49:54  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        CONTROL      1000 PPM     3000 PPM     10000PPM                                  
                                                   FEMALE       LOW FEM      MID FEM      HIGH FEM                                  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
  Animals Initially in Study                          60           60           60           60                                     
  Scheduled Sacrifice                                 10           10           10           10                                     
  Early Deaths                                                                                                                      
    Moribund Sacrifice                                 5            8            1            3                                     
    Natural Death                                      4            2            2            5                                     
    Moribund                                           3                                      2                                     
    Accidently Killed                                                                         1                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                37           40           46           38                                     
  Missing                                              1                         1            1                                     
  Animals Examined Microscopically                    49           50           49           49                                     
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Gallbladder                                        (47)         (45)         (46)         (42)                                   
   Intestine Large, Cecum                             (47)         (50)         (48)         (45)                                   
   Intestine Small, Duodenum                          (46)         (50)         (48)         (46)                                   
      Hepatocholangiocarcinoma, Metastatic, Liver                   1 (2%)                                                          
   Intestine Small, Jejunum                           (47)         (49)         (48)         (45)                                   
      Carcinoma                                                                  1 (2%)                                             
   Intestine Small, Ileum                             (48)         (50)         (48)         (45)                                   
      Carcinoma                                                                               1 (2%)                                
   Liver                                              (49)         (50)         (49)         (49)                                   
      Hemangioma                                       1 (2%)                                                                       
      Hemangiosarcoma                                  1 (2%)       3 (6%)                    1 (2%)                                
      Hemangiosarcoma, Metastatic, Skin                             1 (2%)                                                          
      Hepatoblastoma                                                                          1 (2%)                                
      Hepatocellular Carcinoma                         5 (10%)      5 (10%)      6 (12%)     12 (24%)                               
      Hepatocellular Adenoma                           6 (12%)      8 (16%)      9 (18%)      6 (12%)                               
      Hepatocellular Adenoma, Multiple                 1 (2%)       4 (8%)       8 (16%)     35 (71%)                               
      Hepatocholangiocarcinoma                                      1 (2%)                                                          
      Histiocytic Sarcoma                                           1 (2%)                                                          
      Osteosarcoma, Metastatic, Bone                                1 (2%)                                                          
      Osteosarcoma, Metastatic, Nose                                             1 (2%)                                             
      Sarcoma Stromal, Metastatic, Uterus              1 (2%)                                                                       
   Mesentery                                          (6)          (5)          (3)          (5)                                    
      Hepatocholangiocarcinoma, Metastatic, Liver                   1 (20%)                                                         
      Histiocytic Sarcoma                                           1 (20%)                                                         
   Pancreas                                           (49)         (50)         (48)         (49)                                   
      Hepatocholangiocarcinoma, Metastatic, Liver                   1 (2%)                                                          
   Salivary Glands                                    (49)         (50)         (49)         (48)                                   
   Stomach, Forestomach                               (49)         (50)         (48)         (49)                                   
                                                             Page   2                                                               
NTP Experiment-Test: 05130-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/16/94  
Route: DOSED FEED                                                                                                 Time: 23:49:54  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        CONTROL      1000 PPM     3000 PPM     10000PPM                                  
                                                   FEMALE       LOW FEM      MID FEM      HIGH FEM                                  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - cont                                                                                                            
      Hepatocholangiocarcinoma, Metastatic, Liver                   1 (2%)                                                          
      Squamous Cell Papilloma                                                                 1 (2%)                                
   Stomach, Glandular                                 (49)         (50)         (48)         (49)                                   
      Hepatocholangiocarcinoma, Metastatic, Liver                   1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (49)         (50)         (49)         (49)                                   
      Histiocytic Sarcoma                                           1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (49)         (49)         (48)         (48)                                   
      Adenoma                                                                                 1 (2%)                                
   Adrenal Medulla                                    (49)         (49)         (48)         (47)                                   
      Pheochromocytoma Benign                                       1 (2%)       2 (4%)                                             
   Pituitary Gland                                    (48)         (50)         (49)         (45)                                   
      Pars Distalis, Adenoma                           2 (4%)       6 (12%)      2 (4%)                                             
   Thyroid Gland                                      (49)         (50)         (49)         (49)                                   
      Follicular Cell, Adenoma                                      1 (2%)                    1 (2%)                                
      Follicular Cell, Carcinoma                                    1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   Tissue NOS                                         (1)          (2)                       (1)                                    
      Carcinoma, Metastatic, Thyroid Gland                          1 (50%)                                                         
      Sarcoma, Metastatic, Skeletal Muscle                                                    1 (100%)                              
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Ovary                                              (47)         (50)         (48)         (49)                                   
      Choriocarcinoma                                                                         1 (2%)                                
      Cystadenoma                                      2 (4%)       1 (2%)                    1 (2%)                                
      Granulosa Cell Tumor Benign                                   1 (2%)       2 (4%)                                             
      Hemangioma                                                                 1 (2%)       1 (2%)                                
      Teratoma Benign                                  1 (2%)                    1 (2%)       1 (2%)                                
      Thecoma Benign                                                             1 (2%)                                             
   Uterus                                             (49)         (50)         (49)         (49)                                   
      Deciduoma Benign                                                                        1 (2%)                                
      Hemangioma                                                                              1 (2%)                                
      Hemangiosarcoma                                                                         1 (2%)                                
      Hemangiosarcoma, Metastatic, Bone Marrow                      1 (2%)                                                          
      Histiocytic Sarcoma                                           1 (2%)                                                          
                                                             Page   3                                                               
NTP Experiment-Test: 05130-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/16/94  
Route: DOSED FEED                                                                                                 Time: 23:49:54  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        CONTROL      1000 PPM     3000 PPM     10000PPM                                  
                                                   FEMALE       LOW FEM      MID FEM      HIGH FEM                                  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
GENITAL SYSTEM - cont                                                                                                               
      Leiomyoma                                        1 (2%)                                 1 (2%)                                
      Polyp Stromal                                                              1 (2%)       1 (2%)                                
      Sarcoma Stromal                                  1 (2%)       1 (2%)       1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (49)         (50)         (49)         (49)                                   
      Vertebral, Hemangiosarcoma                                    1 (2%)                                                          
   Lymph Node                                         (9)          (10)         (8)          (7)                                    
      Bronchial, Hepatocellular Carcinoma,                                                                                          
          Metastatic, Liver                                         1 (10%)                                                         
      Bronchial, Osteosarcoma, Metastatic, Bone                     1 (10%)                                                         
      Iliac, Sarcoma Stromal, Metastatic, Uterus       1 (11%)                                                                      
      Lumbar, Hepatocholangiocarcinoma, Metastatic,                                                                                 
           Liver                                                    1 (10%)                                                         
      Mediastinal, Hepatocholangiocarcinoma,                                                                                        
          Metastatic, Liver                                         1 (10%)                                                         
      Renal, Hepatocholangiocarcinoma, Metastatic,                                                                                  
           Liver                                                    1 (10%)                                                         
   Lymph Node, Mandibular                             (49)         (48)         (48)         (47)                                   
   Lymph Node, Mesenteric                             (46)         (48)         (47)         (46)                                   
      Hepatocholangiocarcinoma, Metastatic, Liver                   1 (2%)                                                          
   Spleen                                             (48)         (50)         (49)         (49)                                   
      Hemangiosarcoma                                               1 (2%)                                                          
   Thymus                                             (46)         (48)         (48)         (46)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (49)         (49)         (49)         (48)                                   
      Adenoma                                          1 (2%)                                                                       
   Skin                                               (49)         (50)         (49)         (49)                                   
      Squamous Cell Carcinoma                                                                 1 (2%)                                
      Subcutaneous Tissue, Hemangiosarcoma                          2 (4%)                                                          
      Subcutaneous Tissue, Sarcoma                                               1 (2%)                                             
      Subcutaneous Tissue, Schwannoma Malignant                                  1 (2%)       1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (49)         (50)         (49)         (49)                                   
      Dorsal, Osteosarcoma                                          1 (2%)                                                          
      Vertebra, Hemangiosarcoma, Extension                          1 (2%)                                                          
   Skeletal Muscle                                                 (3)                       (1)                                    
      Back, Hemangiosarcoma, Extension                              1 (33%)                                                         
      Back, Sarcoma                                                                           1 (100%)                              
                                                             Page   4                                                               
NTP Experiment-Test: 05130-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/16/94  
Route: DOSED FEED                                                                                                 Time: 23:49:54  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        CONTROL      1000 PPM     3000 PPM     10000PPM                                  
                                                   FEMALE       LOW FEM      MID FEM      HIGH FEM                                  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
MUSCULOSKELETAL SYSTEM - cont                                                                                                       
      Diaphragm, Hepatocholangiocarcinoma,                                                                                          
          Metastatic, Liver                                         1 (33%)                                                         
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (49)         (50)         (49)         (49)                                   
      Alveolar/Bronchiolar Adenoma                     5 (10%)      5 (10%)      4 (8%)       1 (2%)                                
      Alveolar/Bronchiolar Adenoma, Multiple                        2 (4%)                                                          
      Hepatocellular Carcinoma, Metastatic, Liver      1 (2%)       1 (2%)       2 (4%)       1 (2%)                                
      Hepatocholangiocarcinoma, Metastatic, Liver                   1 (2%)                                                          
      Histiocytic Sarcoma                                           1 (2%)                                                          
      Osteosarcoma, Metastatic, Bone                                1 (2%)                                                          
      Osteosarcoma, Metastatic, Nose                                             1 (2%)                                             
      Sarcoma Stromal, Metastatic, Uterus              1 (2%)                                                                       
   Nose                                               (49)         (50)         (49)         (49)                                   
      Osteosarcoma                                                               1 (2%)                                             
   Trachea                                            (49)         (50)         (49)         (49)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Harderian Gland                                    (3)                       (2)                                                 
      Adenoma                                          3 (100%)                  2 (100%)                                           
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (49)         (50)         (49)         (49)                                   
      Bilateral, Osteosarcoma, Metastatic, Bone                     1 (2%)                                                          
   Urinary Bladder                                    (48)         (50)         (48)         (49)                                   
      Histiocytic Sarcoma                                           1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
   Multiple Organs                                   *(49)        *(50)        *(49)        *(49)                                   
      Histiocytic Sarcoma                                           1 (2%)                                                          
      Lymphoma Malignant Histiocytic                   1 (2%)       2 (4%)                    1 (2%)                                
      Lymphoma Malignant Lymphocytic                   4 (8%)       7 (14%)      5 (10%)      2 (4%)                                
      Lymphoma Malignant Mixed                         1 (2%)       2 (4%)       2 (4%)       2 (4%)                                
      Lymphoma Malignant Undifferentiated Cell         1 (2%)                    1 (2%)                                             
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                             Page   5                                                               
NTP Experiment-Test: 05130-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/16/94  
Route: DOSED FEED                                                                                                 Time: 23:49:54  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        CONTROL      1000 PPM     3000 PPM     10000PPM                                  
                                                   FEMALE       LOW FEM      MID FEM      HIGH FEM                                  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
 SYSTEMIC LESIONS - cont                                                                                                            
   Multiple Organs                                   *(49)        *(50)        *(49)        *(49)                                   
____________________________________________________________________________________________________________________________________
                                                             Page   6                                                               
NTP Experiment-Test: 05130-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/16/94  
Route: DOSED FEED                                                                                                 Time: 23:49:54  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        CONTROL      1000 PPM     3000 PPM     10000PPM                                  
                                                   FEMALE       LOW FEM      MID FEM      HIGH FEM                                  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)            29          35          34          46                                       
     Total Primary Neoplasms                           37          59          52          78                                       
                                                                                                                                    
   Total Animals with Benign Neoplasms                 20          21          26          42                                       
     Total Benign Neoplasms                            23          29          33          53                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms              14          24          17          24                                       
     Total Malignant Neoplasms                         14          30          19          25                                       
                                                                                                                                    
   Total Animals with Metastatic Neoplasms              2           6           3           2                                       
     Total Metastatic Neoplasm                          4          20           4           2                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms                                                                                           
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                                                                                              
     Total Uncertain Neoplasms                                                                                                      
                                                                                                                                    
____________________________________________________________________________________________________________________________________
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                             Page   7                                                               
NTP Experiment-Test: 05130-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/16/94  
Route: DOSED FEED                                                                                                 Time: 23:49:54  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          CONTROL      1000 PPM     3000 PPM     10000PPM                                  
                                                   MALE         LOW MALE     MID MALE     HIGHMALE                                  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
  Animals Initially in Study                          60           60           60           60                                     
  Scheduled Sacrifice                                  9           10           10           10                                     
  Early Deaths                                                                                                                      
    Accidently Killed                                  1                                                                            
    Natural Death                                      3            2            5            4                                     
    Moribund Sacrifice                                 2            2            3            1                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                44           46           42           45                                     
  Missing                                              1                                                                            
  Animals Examined Microscopically                    50           50           50           50                                     
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Gallbladder                                        (45)         (47)         (46)         (47)                                   
   Intestine Large, Colon                             (48)         (50)         (50)         (49)                                   
   Intestine Large, Cecum                             (48)         (49)         (48)         (48)                                   
   Intestine Small, Duodenum                          (48)         (48)         (47)         (49)                                   
      Adenoma                                                       1 (2%)                                                          
   Intestine Small, Jejunum                           (49)         (49)         (49)         (49)                                   
      Carcinoma                                        1 (2%)                                 3 (6%)                                
   Intestine Small, Ileum                             (49)         (49)         (47)         (48)                                   
   Liver                                              (50)         (50)         (50)         (50)                                   
      Hemangioma                                                                              1 (2%)                                
      Hemangiosarcoma                                                            1 (2%)       1 (2%)                                
      Hemangiosarcoma, Metastatic, Spleen                           1 (2%)                    1 (2%)                                
      Hepatoblastoma                                                                          1 (2%)                                
      Hepatocellular Carcinoma                         7 (14%)      3 (6%)       8 (16%)     17 (34%)                               
      Hepatocellular Adenoma                          12 (24%)      9 (18%)      7 (14%)      9 (18%)                               
      Hepatocellular Adenoma, Multiple                 4 (8%)      10 (20%)     10 (20%)     31 (62%)                               
      Histiocytic Sarcoma                              1 (2%)                                 1 (2%)                                
      Mast Cell Tumor Malignant, Metastatic, Spleen    1 (2%)                                                                       
      Sarcoma, Metastatic, Uncertain Primary Site                   1 (2%)                                                          
   Mesentery                                          (3)          (1)                                                              
      Sarcoma, Metastatic, Uncertain Primary Site                   1 (100%)                                                        
   Pancreas                                           (49)         (50)         (50)         (50)                                   
      Sarcoma, Metastatic, Uncertain Primary Site                   1 (2%)                                                          
   Salivary Glands                                    (49)         (50)         (50)         (50)                                   
   Stomach, Forestomach                               (50)         (50)         (50)         (50)                                   
      Squamous Cell Papilloma                                       1 (2%)                    1 (2%)                                
   Stomach, Glandular                                 (50)         (50)         (50)         (50)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                             Page   8                                                               
NTP Experiment-Test: 05130-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/16/94  
Route: DOSED FEED                                                                                                 Time: 23:49:54  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          CONTROL      1000 PPM     3000 PPM     10000PPM                                  
                                                   MALE         LOW MALE     MID MALE     HIGHMALE                                  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (50)         (50)         (50)         (50)                                   
      Hemangiosarcoma                                  1 (2%)                                                                       
      Sarcoma                                                                    1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (50)         (50)         (50)         (50)                                   
      Adenoma                                                       3 (6%)       1 (2%)                                             
      Capsule, Adenoma                                              1 (2%)                                                          
   Pituitary Gland                                    (49)         (48)         (48)         (48)                                   
   Thyroid Gland                                      (50)         (50)         (50)         (50)                                   
      Follicular Cell, Adenoma                         2 (4%)       2 (4%)                    2 (4%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   Tissue NOS                                         (1)          (2)          (1)                                                 
      Sarcoma, Metastatic, Uncertain Primary Site                   1 (50%)                                                         
      Abdominal, Hemangiosarcoma, Metastatic,                                                                                       
          Spleen                                                    1 (50%)                                                         
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Ductus Deferens                                                                           (1)                                    
   Epididymis                                         (50)         (50)         (50)         (50)                                   
      Histiocytic Sarcoma                              1 (2%)                                                                       
      Sarcoma                                          1 (2%)       1 (2%)                                                          
   Preputial Gland                                    (29)         (23)         (23)         (21)                                   
   Prostate                                           (50)         (49)         (50)         (50)                                   
      Histiocytic Sarcoma                              1 (2%)                                                                       
   Seminal Vesicle                                    (50)         (50)         (50)         (50)                                   
   Testes                                             (50)         (50)         (50)         (50)                                   
      Histiocytic Sarcoma                              1 (2%)                                                                       
      Interstitial Cell, Adenoma                                    1 (2%)       1 (2%)       1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (50)         (50)         (50)         (50)                                   
      Histiocytic Sarcoma                              1 (2%)                                                                       
      Mast Cell Tumor Malignant, Metastatic, Spleen    1 (2%)                                                                       
   Lymph Node                                         (4)          (1)          (2)          (3)                                    
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                  1 (50%)                                            
                                                             Page   9                                                               
NTP Experiment-Test: 05130-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/16/94  
Route: DOSED FEED                                                                                                 Time: 23:49:54  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          CONTROL      1000 PPM     3000 PPM     10000PPM                                  
                                                   MALE         LOW MALE     MID MALE     HIGHMALE                                  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - cont                                                                                                         
      Bronchial, Alveolar/Bronchiolar Carcinoma,                                                                                    
           Metastatic, Lung                                                      1 (50%)                                            
      Mediastinal, Alveolar/Bronchiolar Carcinoma,                                                                                  
           Metastatic, Lung                                                      1 (50%)                                            
   Lymph Node, Mandibular                             (46)         (49)         (49)         (48)                                   
   Lymph Node, Mesenteric                             (48)         (49)         (50)         (50)                                   
      Sarcoma, Metastatic, Uncertain Primary Site                   1 (2%)                                                          
   Spleen                                             (49)         (50)         (50)         (50)                                   
      Hemangioma                                                    1 (2%)                                                          
      Hemangiosarcoma                                               1 (2%)                    1 (2%)                                
      Histiocytic Sarcoma                                                                     1 (2%)                                
      Mast Cell Tumor Malignant                        1 (2%)                                                                       
      Sarcoma, Metastatic, Uncertain Primary Site                   1 (2%)                                                          
   Thymus                                             (49)         (45)         (46)         (42)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Skin                                               (50)         (50)         (50)         (50)                                   
      Subcutaneous Tissue, Fibroma                                  1 (2%)                                                          
      Subcutaneous Tissue, Hemangioma                                                         1 (2%)                                
      Subcutaneous Tissue, Hemangiosarcoma                                       1 (2%)                                             
      Subcutaneous Tissue, Lipoma                      1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Skeletal Muscle                                                 (1)                                                              
      Sarcoma                                                       1 (100%)                                                        
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Adenoma                    12 (24%)      8 (16%)      7 (14%)      3 (6%)                                
      Alveolar/Bronchiolar Adenoma, Multiple           1 (2%)       1 (2%)                    1 (2%)                                
      Alveolar/Bronchiolar Carcinoma                   1 (2%)       3 (6%)       2 (4%)       1 (2%)                                
      Hepatocellular Carcinoma, Metastatic, Liver      1 (2%)                    3 (6%)       3 (6%)                                
      Sarcoma, Metastatic, Uncertain Primary Site                   1 (2%)                                                          
      Sarcoma, Metastatic, Skeletal Muscle                          1 (2%)                                                          
   Nose                                               (50)         (50)         (50)         (50)                                   
____________________________________________________________________________________________________________________________________
                                                             Page  10                                                               
NTP Experiment-Test: 05130-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/16/94  
Route: DOSED FEED                                                                                                 Time: 23:49:54  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          CONTROL      1000 PPM     3000 PPM     10000PPM                                  
                                                   MALE         LOW MALE     MID MALE     HIGHMALE                                  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Ear                                                (2)                                                                           
      Pinna, Sarcoma                                   1 (50%)                                                                      
   Harderian Gland                                    (3)          (4)          (4)                                                 
      Adenoma                                          3 (100%)     3 (75%)      4 (100%)                                           
      Bilateral, Adenoma                                            1 (25%)                                                         
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (50)         (50)         (50)         (50)                                   
      Renal Tubule, Adenoma                                         2 (4%)       1 (2%)       1 (2%)                                
      Renal Tubule, Carcinoma                                       1 (2%)                                                          
   Urinary Bladder                                    (50)         (50)         (50)         (50)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
   Multiple Organs                                   *(50)        *(50)        *(50)        *(50)                                   
      Histiocytic Sarcoma                              1 (2%)                                 1 (2%)                                
      Lymphoma Malignant Lymphocytic                   2 (4%)       1 (2%)       2 (4%)       2 (4%)                                
      Lymphoma Malignant Mixed                                      3 (6%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                             Page  11                                                               
NTP Experiment-Test: 05130-02  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/16/94  
Route: DOSED FEED                                                                                                 Time: 23:49:54  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          CONTROL      1000 PPM     3000 PPM     10000PPM                                  
                                                   MALE         LOW MALE     MID MALE     HIGHMALE                                  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)            31          37          28          46                                       
     Total Primary Neoplasms                           51          59          46          78                                       
                                                                                                                                    
   Total Animals with Benign Neoplasms                 25          31          21          42                                       
     Total Benign Neoplasms                            35          45          31          51                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms              14          14          12          24                                       
     Total Malignant Neoplasms                         16          14          15          27                                       
                                                                                                                                    
   Total Animals with Metastatic Neoplasms              2           3           4           4                                       
     Total Metastatic Neoplasm                          3          10           6           4                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms                           1                                                               
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                                                                                              
     Total Uncertain Neoplasms                                                                                                      
                                                                                                                                    
____________________________________________________________________________________________________________________________________
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                             Page  12                                                               
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