National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/2236

TDMS Study 05130-03 Pathology Tables



                              INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)
                                                       C. I. DIRECT BLUE 218


NTP Experiment-Test: 05130-03                                                                                     Report: PEIRPT05
Study Type: CHRONIC                                                                                               Date: 09/17/94
Route: DOSED FEED                                                                                                 Time: 01:29:35




       Facility:  Microbiological Associates

       Chemical CAS #:  10401-500

       Lock Date:  11/21/91

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All
































a  Number of animals examined microscopically at site and number of animals with lesion

                                                             Page   1

NTP Experiment-Test: 05130-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/17/94  
Route: DOSED FEED                                                                                                 Time: 01:29:35  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                   0 PPM        1000 PPM     3000 PPM     10000PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
  Animals Initially in Study                          60           60           60           60                                     
  Scheduled Sacrifice                                  9            9           10           10                                     
  Early Deaths                                                                                                                      
    Moribund Sacrifice                                12           15           13           16                                     
    Natural Death                                      4            7            6            9                                     
  Survivors                                                                                                                         
    Moribund Sacrifice                                              1                                                               
    Terminal Sacrifice                                35           28           31           25                                     
  Animals Examined Microscopically                    50           50           50           50                                     
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Esophagus                                          (50)         (50)         (49)         (50)                                   
      Lipoma                                                        1 (2%)                                                          
   Intestine Large, Colon                             (49)         (47)         (49)         (48)                                   
   Intestine Large, Rectum                            (48)         (47)         (49)         (48)                                   
   Intestine Large, Cecum                             (48)         (47)         (48)         (48)                                   
   Intestine Small, Duodenum                          (48)         (48)         (50)         (45)                                   
   Intestine Small, Jejunum                           (48)         (45)         (49)         (47)                                   
      Carcinoma                                                                  1 (2%)                                             
   Intestine Small, Ileum                             (48)         (46)         (48)         (46)                                   
   Liver                                              (50)         (50)         (50)         (50)                                   
      Sarcoma, Metastatic, Skin                                     1 (2%)                                                          
   Mesentery                                          (8)          (6)          (3)          (6)                                    
      Sarcoma, Metastatic, Skin                                     1 (17%)                                                         
      Schwannoma Malignant, Metastatic, Uterus                      1 (17%)                                                         
      Fat, Sarcoma                                                               1 (33%)                                            
   Pancreas                                           (50)         (48)         (50)         (48)                                   
   Pharynx                                            (1)          (1)                       (2)                                    
      Palate, Squamous Cell Papilloma                  1 (100%)     1 (100%)                  2 (100%)                              
   Salivary Glands                                    (50)         (50)         (50)         (50)                                   
   Stomach, Forestomach                               (50)         (49)         (50)         (49)                                   
      Squamous Cell Papilloma                                                                 1 (2%)                                
   Stomach, Glandular                                 (50)         (48)         (50)         (48)                                   
      Sarcoma, Metastatic, Skin                                     1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                             Page   2                                                               
NTP Experiment-Test: 05130-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/17/94  
Route: DOSED FEED                                                                                                 Time: 01:29:35  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                   0 PPM        1000 PPM     3000 PPM     10000PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (50)         (50)         (50)         (50)                                   
      Schwannoma NOS                                                1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (50)         (50)         (50)         (49)                                   
      Carcinoma, Metastatic, Thyroid Gland                                                    1 (2%)                                
   Adrenal Medulla                                    (49)         (50)         (47)         (49)                                   
      Pheochromocytoma Benign                          1 (2%)                                 2 (4%)                                
      Bilateral, Pheochromocytoma Benign                                         1 (2%)       1 (2%)                                
   Islets, Pancreatic                                 (50)         (48)         (50)         (48)                                   
      Adenoma                                                       1 (2%)       2 (4%)                                             
   Parathyroid Gland                                  (45)         (43)         (39)         (44)                                   
   Pituitary Gland                                    (50)         (47)         (49)         (47)                                   
      Pars Distalis, Adenoma                          26 (52%)     29 (62%)     30 (61%)     20 (43%)                               
      Pars Distalis, Adenoma, Multiple                 2 (4%)       2 (4%)       2 (4%)       1 (2%)                                
      Pars Distalis, Carcinoma                         2 (4%)                    1 (2%)       2 (4%)                                
      Pars Intermedia, Adenoma                                      1 (2%)                                                          
   Thyroid Gland                                      (50)         (50)         (49)         (49)                                   
      Bilateral, C-Cell, Adenoma                       1 (2%)                                 1 (2%)                                
      C-Cell, Adenoma                                  2 (4%)       4 (8%)       2 (4%)       2 (4%)                                
      C-Cell, Carcinoma                                1 (2%)                                 3 (6%)                                
      Follicular Cell, Adenoma                                                   2 (4%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   Tissue NOS                                                      (1)                                                              
      Schwannoma Malignant, Metastatic, Uterus                      1 (100%)                                                        
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Clitoral Gland                                     (47)         (48)         (49)         (47)                                   
      Adenoma                                          2 (4%)                    2 (4%)       2 (4%)                                
      Carcinoma                                                     1 (2%)                                                          
      Squamous Cell Carcinoma                                                                 1 (2%)                                
   Ovary                                              (50)         (50)         (50)         (50)                                   
      Granulosa Cell Tumor Benign                                   1 (2%)                                                          
      Sarcoma, Metastatic, Skin                                     1 (2%)                                                          
   Uterus                                             (50)         (50)         (50)         (50)                                   
      Adenoma                                          1 (2%)       1 (2%)                                                          
      Leiomyosarcoma                                                             1 (2%)                                             
      Polyp Stromal                                    1 (2%)      12 (24%)     10 (20%)     10 (20%)                               
      Sarcoma                                                       1 (2%)                                                          
                                                             Page   3                                                               
NTP Experiment-Test: 05130-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/17/94  
Route: DOSED FEED                                                                                                 Time: 01:29:35  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                   0 PPM        1000 PPM     3000 PPM     10000PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
GENITAL SYSTEM - cont                                                                                                               
      Schwannoma Malignant                                          1 (2%)                                                          
      Cervix, Schwannoma Malignant, Metastatic,                                                                                     
           Uterus                                                   1 (2%)                                                          
   Vagina                                             (1)          (1)          (2)          (3)                                    
      Fibroma                                                                                 1 (33%)                               
      Leiomyosarcoma                                   1 (100%)                                                                     
      Schwannoma Malignant, Metastatic, Uterus                      1 (100%)                                                        
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (49)         (49)         (50)         (49)                                   
   Lymph Node                                         (7)          (12)         (6)          (14)                                   
   Lymph Node, Mandibular                             (49)         (50)         (50)         (50)                                   
      Carcinoma, Metastatic, Thyroid Gland                                                    1 (2%)                                
   Lymph Node, Mesenteric                             (50)         (50)         (50)         (50)                                   
      Sarcoma, Metastatic, Skin                                     1 (2%)                                                          
      Schwannoma Malignant, Metastatic, Uterus                      1 (2%)                                                          
   Spleen                                             (50)         (49)         (50)         (48)                                   
   Thymus                                             (48)         (49)         (42)         (45)                                   
      Thymoma Benign                                                                          1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (50)         (50)         (50)         (50)                                   
      Adenoacanthoma                                                                          1 (2%)                                
      Adenocarcinoma                                   3 (6%)       1 (2%)       2 (4%)       1 (2%)                                
      Adenoma                                                                                 1 (2%)                                
      Fibroadenoma                                    14 (28%)     11 (22%)     16 (32%)     10 (20%)                               
      Fibroadenoma, Multiple                           5 (10%)      1 (2%)       3 (6%)                                             
      Sarcoma, Metastatic, Skin                                     1 (2%)                                                          
   Skin                                               (50)         (50)         (50)         (50)                                   
      Keratoacanthoma                                               1 (2%)                                                          
      Subcutaneous Tissue, Fibroma                                                            1 (2%)                                
      Subcutaneous Tissue, Fibrosarcoma                             1 (2%)       1 (2%)                                             
      Subcutaneous Tissue, Sarcoma                                  1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Skeletal Muscle                                    (1)                                    (1)                                    
      Back, Sarcoma                                                                           1 (100%)                              
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                             Page   4                                                               
NTP Experiment-Test: 05130-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/17/94  
Route: DOSED FEED                                                                                                 Time: 01:29:35  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                   0 PPM        1000 PPM     3000 PPM     10000PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (50)         (50)         (50)         (50)                                   
      Astrocytoma NOS                                                            1 (2%)                                             
      Carcinoma, Metastatic, Pituitary Gland           1 (2%)                                 1 (2%)                                
      Ependymoma Benign                                                          1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Adenoma                                  1 (2%)                    2 (4%)                                
      Carcinoma, Metastatic, Thyroid Gland             1 (2%)                                 2 (4%)                                
      Fibrosarcoma, Metastatic, Ear                                              1 (2%)                                             
      Sarcoma, Metastatic, Skin                                     1 (2%)                                                          
   Nose                                               (50)         (50)         (50)         (50)                                   
   Trachea                                            (50)         (50)         (49)         (50)                                   
      Carcinoma, Metastatic, Thyroid Gland                                                    2 (4%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Ear                                                                          (1)                                                 
      Pinna, Fibrosarcoma                                                        1 (100%)                                           
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (50)         (50)         (50)         (50)                                   
      Carcinoma                                                                               1 (2%)                                
      Lipoma                                                        1 (2%)                                                          
   Urinary Bladder                                    (48)         (48)         (50)         (49)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
   Multiple Organs                                   *(50)        *(50)        *(50)        *(50)                                   
      Leukemia Mononuclear                            11 (22%)     16 (32%)      9 (18%)     16 (32%)                               
      Lymphoma Malignant Lymphocytic                                1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                             Page   5                                                               
NTP Experiment-Test: 05130-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/17/94  
Route: DOSED FEED                                                                                                 Time: 01:29:35  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                   0 PPM        1000 PPM     3000 PPM     10000PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)            45          46          46          45                                       
     Total Primary Neoplasms                           74          92          89          84                                       
                                                                                                                                    
   Total Animals with Benign Neoplasms                 37          39          39          38                                       
     Total Benign Neoplasms                            56          68          71          58                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms              17          22          17          22                                       
     Total Malignant Neoplasms                         18          23          17          26                                       
                                                                                                                                    
   Total Animals with Metastatic Neoplasms              2           2           1           3                                       
     Total Metastatic Neoplasm                          2          12           1           7                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms                                                                                           
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                              1           1                                                   
     Total Uncertain Neoplasms                                      1           1                                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                             Page   6                                                               
NTP Experiment-Test: 05130-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/17/94  
Route: DOSED FEED                                                                                                 Time: 01:29:35  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS MALE                     0 PPM        1000 PPM     3000 PPM     10000PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
  Animals Initially in Study                          60           60           60           60                                     
  Scheduled Sacrifice                                 10           10           10            9                                     
  Early Deaths                                                                                                                      
    Natural Death                                      7            3            5            9                                     
    Moribund Sacrifice                                13           22           16           18                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                30           25           29           24                                     
  Animals Examined Microscopically                    50           50           50           50                                     
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Esophagus                                          (50)         (50)         (50)         (50)                                   
   Intestine Large, Colon                             (50)         (50)         (50)         (50)                                   
      Leiomyoma                                        1 (2%)                                                                       
      Sarcoma, Metastatic, Spleen                                                1 (2%)                                             
   Intestine Large, Rectum                            (46)         (49)         (49)         (50)                                   
   Intestine Large, Cecum                             (46)         (49)         (49)         (49)                                   
      Adenocarcinoma                                                                          1 (2%)                                
   Intestine Small, Duodenum                          (49)         (49)         (50)         (48)                                   
      Sarcoma, Metastatic, Spleen                                                1 (2%)                                             
   Intestine Small, Jejunum                           (47)         (49)         (49)         (46)                                   
   Intestine Small, Ileum                             (46)         (49)         (48)         (47)                                   
   Liver                                              (50)         (50)         (50)         (50)                                   
      Hepatocellular Adenoma                           4 (8%)       5 (10%)      1 (2%)       6 (12%)                               
      Hepatocellular Adenoma, Multiple                 1 (2%)       1 (2%)       1 (2%)                                             
      Histiocytic Sarcoma                                                                     2 (4%)                                
      Sarcoma, Metastatic, Spleen                                                1 (2%)                                             
   Mesentery                                          (5)          (5)          (7)          (4)                                    
      Sarcoma, Metastatic, Spleen                                                1 (14%)                                            
   Pancreas                                           (50)         (50)         (50)         (50)                                   
      Sarcoma, Metastatic, Spleen                                                1 (2%)                                             
   Pharynx                                                         (1)                       (9)                                    
      Palate, Basosquamous Tumor Benign                                                       1 (11%)                               
      Palate, Squamous Cell Carcinoma                                                         1 (11%)                               
      Palate, Squamous Cell Papilloma                                                         5 (56%)                               
   Salivary Glands                                    (50)         (50)         (50)         (50)                                   
      Carcinoma                                                                  1 (2%)                                             
   Stomach, Forestomach                               (50)         (50)         (50)         (50)                                   
      Sarcoma, Metastatic, Spleen                                                1 (2%)                                             
      Squamous Cell Carcinoma                                                    1 (2%)                                             
      Squamous Cell Papilloma                                                    2 (4%)       1 (2%)                                
   Stomach, Glandular                                 (50)         (50)         (50)         (50)                                   
                                                             Page   7                                                               
NTP Experiment-Test: 05130-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/17/94  
Route: DOSED FEED                                                                                                 Time: 01:29:35  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS MALE                     0 PPM        1000 PPM     3000 PPM     10000PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - cont                                                                                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (50)         (50)         (50)         (50)                                   
      Schwannoma Malignant                             1 (2%)                    1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (49)         (50)         (50)         (50)                                   
      Adenoma                                                                                 1 (2%)                                
      Sarcoma, Metastatic, Spleen                                                1 (2%)                                             
   Adrenal Medulla                                    (49)         (50)         (50)         (50)                                   
      Mixed Tumor Malignant                                                      1 (2%)                                             
      Pheochromocytoma Malignant                       2 (4%)                    2 (4%)       1 (2%)                                
      Pheochromocytoma Benign                         14 (29%)      7 (14%)      9 (18%)      7 (14%)                               
      Sarcoma, Metastatic, Spleen                                                1 (2%)                                             
      Bilateral, Pheochromocytoma Benign               1 (2%)       2 (4%)       3 (6%)       5 (10%)                               
   Islets, Pancreatic                                 (50)         (50)         (50)         (50)                                   
      Adenoma                                          4 (8%)       5 (10%)      3 (6%)       7 (14%)                               
      Carcinoma                                                                  1 (2%)                                             
   Parathyroid Gland                                  (43)         (47)         (46)         (45)                                   
      Adenoma                                                       1 (2%)       1 (2%)                                             
   Pituitary Gland                                    (50)         (50)         (48)         (50)                                   
      Pars Distalis, Adenoma                          15 (30%)     14 (28%)     15 (31%)      9 (18%)                               
      Pars Distalis, Adenoma, Multiple                                           1 (2%)       3 (6%)                                
      Pars Distalis, Carcinoma                         2 (4%)                                                                       
   Thyroid Gland                                      (50)         (50)         (50)         (50)                                   
      C-Cell, Adenoma                                  7 (14%)      1 (2%)       4 (8%)       4 (8%)                                
      C-Cell, Carcinoma                                1 (2%)                    1 (2%)       1 (2%)                                
      Follicular Cell, Carcinoma                                                 1 (2%)       1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Epididymis                                         (50)         (50)         (50)         (50)                                   
   Preputial Gland                                    (50)         (50)         (50)         (49)                                   
      Adenoma                                          1 (2%)       3 (6%)       2 (4%)       2 (4%)                                
      Squamous Cell Papilloma                                                                 1 (2%)                                
      Bilateral, Adenoma                               1 (2%)                                                                       
   Prostate                                           (50)         (50)         (50)         (49)                                   
                                                             Page   8                                                               
NTP Experiment-Test: 05130-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/17/94  
Route: DOSED FEED                                                                                                 Time: 01:29:35  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS MALE                     0 PPM        1000 PPM     3000 PPM     10000PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
GENITAL SYSTEM - cont                                                                                                               
   Seminal Vesicle                                    (50)         (50)         (50)         (49)                                   
   Testes                                             (50)         (50)         (50)         (50)                                   
      Sarcoma, Metastatic, Spleen                                                1 (2%)                                             
      Bilateral, Interstitial Cell, Adenoma           41 (82%)     41 (82%)     43 (86%)     33 (66%)                               
      Interstitial Cell, Adenoma                       4 (8%)       5 (10%)      5 (10%)     11 (22%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Blood                                                           (1)                                                              
   Bone Marrow                                        (50)         (50)         (50)         (50)                                   
      Histiocytic Sarcoma                                                                     2 (4%)                                
   Lymph Node                                         (15)         (16)         (23)         (18)                                   
      Pancreatic, Rhabdomyosarcoma, Metastatic,                                                                                     
           Skeletal Muscle                             1 (7%)                                                                       
   Lymph Node, Mandibular                             (48)         (50)         (49)         (50)                                   
   Lymph Node, Mesenteric                             (49)         (50)         (50)         (50)                                   
      Rhabdomyosarcoma, Metastatic, Skeletal Muscle    1 (2%)                                                                       
      Sarcoma, Metastatic, Spleen                                                1 (2%)                                             
   Spleen                                             (50)         (50)         (50)         (49)                                   
      Sarcoma                                                                    1 (2%)       1 (2%)                                
   Thymus                                             (48)         (40)         (49)         (48)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (48)         (49)         (50)         (46)                                   
      Adenocarcinoma                                                                          1 (2%)                                
      Fibroadenoma                                     2 (4%)       2 (4%)       1 (2%)       1 (2%)                                
      Fibroadenoma, Multiple                                        1 (2%)                                                          
      Histiocytic Sarcoma                                                                     1 (2%)                                
   Skin                                               (50)         (50)         (50)         (50)                                   
      Basal Cell Adenoma                               2 (4%)       2 (4%)                                                          
      Basal Cell Carcinoma                                          1 (2%)                                                          
      Basosquamous Tumor Benign                                     1 (2%)                                                          
      Keratoacanthoma                                  1 (2%)       2 (4%)       2 (4%)       3 (6%)                                
      Keratoacanthoma, Multiple                                     1 (2%)                                                          
      Squamous Cell Papilloma                          1 (2%)                                 1 (2%)                                
      Sebaceous Gland, Adenoma                                                   1 (2%)       1 (2%)                                
      Subcutaneous Tissue, Fibroma                     4 (8%)       4 (8%)       5 (10%)      2 (4%)                                
      Subcutaneous Tissue, Fibrosarcoma                                                       1 (2%)                                
      Subcutaneous Tissue, Histiocytic Sarcoma                                                1 (2%)                                
      Subcutaneous Tissue, Neurofibrosarcoma           1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                             Page   9                                                               
NTP Experiment-Test: 05130-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/17/94  
Route: DOSED FEED                                                                                                 Time: 01:29:35  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS MALE                     0 PPM        1000 PPM     3000 PPM     10000PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Skeletal Muscle                                    (2)          (1)                       (1)                                    
      Histiocytic Sarcoma                                                                     1 (100%)                              
      Lipoma                                                        1 (100%)                                                        
      Abdominal, Rhabdomyosarcoma                      1 (50%)                                                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (50)         (50)         (50)         (50)                                   
      Ependymoma Benign                                             1 (2%)                                                          
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 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Adenoma                     2 (4%)                    1 (2%)       2 (4%)                                
      Histiocytic Sarcoma                                                                     1 (2%)                                
      Pheochromocytoma Malignant, Metastatic,                                                                                       
          Adrenal Medulla                                                        1 (2%)                                             
      Sarcoma, Metastatic, Spleen                                                1 (2%)                                             
   Nose                                               (50)         (50)         (49)         (50)                                   
   Trachea                                            (50)         (50)         (50)         (50)                                   
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 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Zymbal's Gland                                     (48)         (48)         (48)         (48)                                   
      Carcinoma                                        1 (2%)       1 (2%)       1 (2%)       1 (2%)                                
      Squamous Cell Papilloma                          1 (2%)                                                                       
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 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (50)         (50)         (50)         (50)                                   
      Renal Tubule, Adenoma                                                      2 (4%)                                             
      Transitional Epithelium, Carcinoma               1 (2%)       1 (2%)                                                          
   Urinary Bladder                                    (50)         (50)         (50)         (50)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
   Multiple Organs                                   *(50)        *(50)        *(50)        *(50)                                   
      Histiocytic Sarcoma                                                                     2 (4%)                                
      Leukemia Mononuclear                            18 (36%)     25 (50%)     23 (46%)     26 (52%)                               
      Mesothelioma Malignant                           1 (2%)       1 (2%)       1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                             Page  10                                                               
NTP Experiment-Test: 05130-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 09/17/94  
Route: DOSED FEED                                                                                                 Time: 01:29:35  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS MALE                     0 PPM        1000 PPM     3000 PPM     10000PPM                                  
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)            49          50          49          49                                       
     Total Primary Neoplasms                          136         129         137         143                                       
                                                                                                                                    
   Total Animals with Benign Neoplasms                 47          50          49          46                                       
     Total Benign Neoplasms                           107         100         102         106                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms              27          27          28          31                                       
     Total Malignant Neoplasms                         29          29          35          37                                       
                                                                                                                                    
   Total Animals with Metastatic Neoplasms              1                       2                                                   
     Total Metastatic Neoplasm                          2                      12                                                   
                                                                                                                                    
   Total Animals with Malignant Neoplasms                                                                                           
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                                                                                              
     Total Uncertain Neoplasms                                                                                                      
                                                                                                                                    
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a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                             Page  11                                                               
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NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.