Share This:
https://ntp.niehs.nih.gov/go/2240

TDMS Study 05130-03 Pathology Tables

NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97
Route: DOSED FEED                                                                                                 Time: 14:07:43




       Facility:  Microbiological Associates

       Chemical CAS #:  10401-500

       Lock Date:  11/21/91

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All






































                                                              Page   1

NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 5| 6| 5| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 2| 7| 4| 7| 6| 7| 7|             
                             DAY ON TEST   | 7| 3| 3| 7| 3| 4| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 7| 3| 2| 3| 6| 3| 3|             
                                           | 3| 4| 2| 1| 2| 2| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 0| 4| 8| 4| 6| 4| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|             
    0 PPM                                  | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
      Mineralization, Focal                |                                                                          |             
                                            __________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                                     2                                    |             
      Basophilic Focus                     | X     X  X     X  X  X  X     X  X  X  X  X        X     X     X  X  X  X|             
      Clear Cell Focus                     |                   X  X              X     X        X     X     X         |             
      Congestion                           |          3                                            2                  |             
      Degeneration, Cystic, Focal          |                                                          1               |             
      Developmental Malformation           |                                                                          |             
      Eosinophilic Focus                   |    X                                                                     |             
      Fatty Change, Diffuse                |                                                 4                        |             
      Fatty Change, Focal                  | 1     1  2  1           1  2  2                                      1  2|             
      Hematopoietic Cell Proliferation     |                                           2                              |             
      Hepatodiaphragmatic Nodule           |                                        X                                 |             
      Hyperplasia, Focal                   |    2                                                                     |             
      Inflammation, Chronic, Focal         |             2              1  2  2                                       |             
      Inflammation, Focal, Granulomatous   |                                                                          |             
      Mixed Cell Focus                     |                                                                          |             
      Bile Duct, Hyperplasia, Focal        |                                                          2              1|             
                                            __________________________________________________________________________|             
   Mesentery                               | +                 +                                            +     +   |             
      Fat, Necrosis, Focal                 | 3                 3                                            2     3   |             
                                            __________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acinus, Atrophy, Diffuse             |                                                                          |             
      Acinus, Atrophy, Focal               |                   2              1  1  1  2  2           2               |             
                                            __________________________________________________________________________|             
   Pharynx                                 |                                                                          |             
                                            __________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acanthosis, Focal                    |          3                                                               |             
      Hyperplasia, Basal Cell, Focal       |          2                                                               |             
      Inflammation, Acute, Focal           |                                                                          |             
      Inflammation, Chronic, Focal         |          3                                                               |             
      Ulcer, Focal                         |          4                                                               |             
      Ulcer                                |                                                                          |             
                                            __________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Erosion, Focal                       |                                                 1                        |             
      Inflammation, Chronic Active, Focal  |                                     3                                    |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page   2                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 5| 6| 5| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 2| 7| 4| 7| 6| 7| 7|             
                             DAY ON TEST   | 7| 3| 3| 7| 3| 4| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 7| 3| 2| 3| 6| 3| 3|             
                                           | 3| 4| 2| 1| 2| 2| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 0| 4| 8| 4| 6| 4| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|             
    0 PPM                                  | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM - cont              |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 1        2     1  2     2     1     1  2           1     1     1     2   |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                                                                          |             
      Hyperplasia, Focal                   |                                                          3               |             
      Hypertrophy, Focal                   |                                                                          |             
      Vacuolization Cytoplasmic, Focal     |          3              2              1        2           2        1  2|             
                                            __________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                            3  2                          2               |             
                                            __________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |                                                    2                     |             
                                            __________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  M  M  +  +  +  +  +  M  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Pars Distalis, Angiectasis, Focal    |                      2                                                   |             
      Pars Distalis, Cyst                  |                   2                                      2  2            |             
      Pars Distalis, Hyperplasia, Focal    |       3              2        2  3                          1  2        1|             
                                            __________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                                  3                                       |             
      C-Cell, Hyperplasia, Diffuse         |                                  2                                       |             
      C-Cell, Hyperplasia, Focal           |                               1                                1        2|             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +|             
      Inflammation, Chronic                |                                                                          |             
      Inflammation, Suppurative            |                                        2                                 |             
      Duct, Dilatation                     |                   3                 2        3                          2|             
                                            __________________________________________________________________________|             
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                          3        2      |             
                                            __________________________________________________________________________|             
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                               1                                          |             
      Cyst, Multiple                       | 3                                                        3               |             
      Dilatation                           |                                                                          |             
      Hypoplasia                           |                                                                          |             
                                            __________________________________________________________________________|             
   Vagina                                  |       +                                                                  |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |          3     2                    2     2                              |             
                                            __________________________________________________________________________|             
   Lymph Node                              | +                                                           +     +      |             
 _____________________________________________________________________________________________________________________|             
                                                             Page   3                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 5| 6| 5| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 2| 7| 4| 7| 6| 7| 7|             
                             DAY ON TEST   | 7| 3| 3| 7| 3| 4| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 7| 3| 2| 3| 6| 3| 3|             
                                           | 3| 4| 2| 1| 2| 2| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 0| 4| 8| 4| 6| 4| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|             
    0 PPM                                  | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
                                           |                                                                          |             
      Mediastinal, Hemorrhage              |                                                                          |             
      Renal, Hemorrhage                    | 2                                                                 2      |             
                                            __________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +|             
      Hemorrhage                           |                2                                      2                  |             
      Hyperplasia, Lymphoid                |                                                                          |             
      Hyperplasia, Plasma Cell             | 1        2                                                               |             
                                            __________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           | 2                 2           2                                          |             
                                            __________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hematopoietic Cell Proliferation     |       4                                                                  |             
                                            __________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                       3                  |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration, Focal                  |                                                          2               |             
      Galactocele                          |          3     3  2  3              2           2  2           2     1   |             
      Hyperplasia, Focal                   |                                                                          |             
      Inflammation, Acute, Focal           |             2                                                            |             
      Inflammation, Chronic, Focal         |                                                                      3   |             
      Duct, Dilatation                     |       1                                                                  |             
                                            __________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst Epithelial Inclusion            |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperostosis                         |                   3        3  2                                          |             
      Inflammation, Acute                  |                                                                          |             
      Femur, Osteoporosis                  |                                                                          |             
                                            __________________________________________________________________________|             
   Skeletal Muscle                         |                   +                                                      |             
      Diaphragm, Ectopic Tissue            |                   X                                                      |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hydrocephalus                        |          3                                                               |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                       3                  |             
      Hemorrhage, Focal                    |                                           1                              |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Histiocyte                       |          2        1                    1  1  1                           |             
      Inflammation, Focal                  | 2     1           1              1  1                          1         |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page   4                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 5| 6| 5| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 2| 7| 4| 7| 6| 7| 7|             
                             DAY ON TEST   | 7| 3| 3| 7| 3| 4| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 5| 3| 7| 3| 2| 3| 6| 3| 3|             
                                           | 3| 4| 2| 1| 2| 2| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 0| 4| 8| 4| 6| 4| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|             
    0 PPM                                  | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
                                           |                                                                          |             
           Focal                           |                                                          2               |             
                                            __________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Fungus                               |                                                                          |             
      Hemorrhage                           |                                                                          |             
      Inflammation, Acute                  |                                     2                                    |             
      Inflammation, Chronic Active         |          2                                            3        2         |             
      Inflammation, Subacute               |                         3                                                |             
                                            __________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Eye                                     |                                              +                           |             
      Phthisis Bulbi                       |                                              4                           |             
      Retina, Atrophy                      |                                                                          |             
                                            __________________________________________________________________________|             
   Zymbal's Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  M  +  +|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage, Focal                    |                         1                                                |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                                                                          |             
      Mineralization, Focal                |       1  2  1  2  1  2     2     1  1  1     1  1  1  1  2  1     1  1  1|             
      Nephropathy                          |    2  1        4  3  2  1  2  1  1  2  1  2  1     2     2     2  2  2  1|             
      Pigmentation, Focal                  | 1  1  1  3  1  2  1     1  1        1  1        3  2           1     1   |             
                                            __________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  M  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page   5                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 4| 7| 7| 7| 7| 7| 7| 6| 7|             
                             DAY ON TEST   | 3| 3| 8| 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 9| 3| 6| 3| 3| 3| 3| 3| 1| 6| 3|             
                                           | 4| 4| 4| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 0| 4| 4| 4| 4| 4| 4| 6| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|             
    0 PPM                                  | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Mineralization, Focal                |                                              1                           |             
                                            __________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |             2                                      2                     |             
      Basophilic Focus                     | X  X        X  X  X  X  X  X  X     X  X  X  X     X  X  X  X  X  X     X|             
      Clear Cell Focus                     |                   X        X           X                       X         |             
      Congestion                           |                                                                          |             
      Degeneration, Cystic, Focal          |                      2                                                   |             
      Developmental Malformation           | X                                                                        |             
      Eosinophilic Focus                   |                               X                                          |             
      Fatty Change, Diffuse                |       3  4                                                           3   |             
      Fatty Change, Focal                  |                               2  1     2                       2  3      |             
      Hematopoietic Cell Proliferation     |             1                    1                                       |             
      Hepatodiaphragmatic Nodule           |                   X  X                       X                           |             
      Hyperplasia, Focal                   |    2                       2                                         3   |             
      Inflammation, Chronic, Focal         |    2        1           1  2  1        1     1                           |             
      Inflammation, Focal, Granulomatous   |                                                             3            |             
      Mixed Cell Focus                     |                                        X                 X               |             
      Bile Duct, Hyperplasia, Focal        |                                  2                                       |             
                                            __________________________________________________________________________|             
   Mesentery                               |          +           +                             +              +      |             
      Fat, Necrosis, Focal                 |          3           3                             3              3      |             
                                            __________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acinus, Atrophy, Diffuse             |                                                                   4      |             
      Acinus, Atrophy, Focal               | 1              2        2     1        2     2           2              2|             
                                            __________________________________________________________________________|             
   Pharynx                                 |                               +                                          |             
                                            __________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acanthosis, Focal                    |                                                                   2      |             
      Hyperplasia, Basal Cell, Focal       |                                                                          |             
      Inflammation, Acute, Focal           |          2                                                        3      |             
      Inflammation, Chronic, Focal         |                                                                          |             
      Ulcer, Focal                         |                                                                   2      |             
      Ulcer                                |                                                 2                        |             
                                            __________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Erosion, Focal                       |                                                                          |             
      Inflammation, Chronic Active, Focal  |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page   6                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 4| 7| 7| 7| 7| 7| 7| 6| 7|             
                             DAY ON TEST   | 3| 3| 8| 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 9| 3| 6| 3| 3| 3| 3| 3| 1| 6| 3|             
                                           | 4| 4| 4| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 0| 4| 4| 4| 4| 4| 4| 6| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|             
    0 PPM                                  | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM - cont              |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 1        2  1                    2  1        2     1  2  2        2      |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                                                          2              2|             
      Hyperplasia, Focal                   | 2  2                                   2                                 |             
      Hypertrophy, Focal                   |                               2              1                           |             
      Vacuolization Cytoplasmic, Focal     |       3  3  2     1  2     1  2  1     2              2           1      |             
                                            __________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          | 2  2                          2           3                              |             
                                            __________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |                                                                          |             
                                            __________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  M  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Pars Distalis, Angiectasis, Focal    |                                                                          |             
      Pars Distalis, Cyst                  | 3              2              3              3     2  3        2         |             
      Pars Distalis, Hyperplasia, Focal    |       2  1              2           2  1                       2         |             
                                            __________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                                                                          |             
      C-Cell, Hyperplasia, Diffuse         |                                                                          |             
      C-Cell, Hyperplasia, Focal           |       2     1  1                 1                       1  2           2|             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Clitoral Gland                          | +  M  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                |             1                    2                                       |             
      Inflammation, Suppurative            |                3                                      3                  |             
      Duct, Dilatation                     |          3                             2     2                    3      |             
                                            __________________________________________________________________________|             
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                          2               |             
                                            __________________________________________________________________________|             
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                            2        3                       1           1|             
      Cyst, Multiple                       |                                                                          |             
      Dilatation                           |                   2     2  1                                             |             
      Hypoplasia                           |                2                                                         |             
                                            __________________________________________________________________________|             
   Vagina                                  |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone Marrow                             | +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |                   2        2                                      2      |             
                                            __________________________________________________________________________|             
   Lymph Node                              |       +  +                                                        +  +   |             
 _____________________________________________________________________________________________________________________|             
                                                             Page   7                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 4| 7| 7| 7| 7| 7| 7| 6| 7|             
                             DAY ON TEST   | 3| 3| 8| 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 9| 3| 6| 3| 3| 3| 3| 3| 1| 6| 3|             
                                           | 4| 4| 4| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 0| 4| 4| 4| 4| 4| 4| 6| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|             
    0 PPM                                  | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
                                           |                                                                          |             
      Mediastinal, Hemorrhage              |                                                                   2      |             
      Renal, Hemorrhage                    |                                                                          |             
                                            __________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                                   2      |             
      Hyperplasia, Lymphoid                |    2                                                                     |             
      Hyperplasia, Plasma Cell             | 2                                                                        |             
                                            __________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |    3                 2                                            1      |             
                                            __________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hematopoietic Cell Proliferation     |                                                                          |             
                                            __________________________________________________________________________|             
   Thymus                                  | +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration, Focal                  |                                                                          |             
      Galactocele                          | 2  3                                   2                          2      |             
      Hyperplasia, Focal                   |                                           2                              |             
      Inflammation, Acute, Focal           |                                                                          |             
      Inflammation, Chronic, Focal         |                                                                          |             
      Duct, Dilatation                     |                                           3                              |             
                                            __________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst Epithelial Inclusion            |                         X                                                |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperostosis                         |                                        4                                3|             
      Inflammation, Acute                  |    2                                                                     |             
      Femur, Osteoporosis                  |          4                                                               |             
                                            __________________________________________________________________________|             
   Skeletal Muscle                         |                                                                          |             
      Diaphragm, Ectopic Tissue            |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hydrocephalus                        |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                                          |             
      Hemorrhage, Focal                    |                                                                          |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Histiocyte                       |                         1     1                       1  1     1         |             
      Inflammation, Focal                  |                   2        1           2                                 |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page   8                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 4| 7| 4| 7| 7| 7| 7| 7| 7| 6| 7|             
                             DAY ON TEST   | 3| 3| 8| 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 9| 3| 6| 3| 3| 3| 3| 3| 1| 6| 3|             
                                           | 4| 4| 4| 1| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 9| 4| 0| 4| 4| 4| 4| 4| 4| 6| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|             
    0 PPM                                  | 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
                                           |                                                                          |             
           Focal                           |                                                                          |             
                                            __________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Fungus                               |                         3                                                |             
      Hemorrhage                           |                                                    2                     |             
      Inflammation, Acute                  |                                                 2                        |             
      Inflammation, Chronic Active         |    2        2           3                                                |             
      Inflammation, Subacute               |                2                                                         |             
                                            __________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Eye                                     |                   +                                                      |             
      Phthisis Bulbi                       |                                                                          |             
      Retina, Atrophy                      |                   3                                                      |             
                                            __________________________________________________________________________|             
   Zymbal's Gland                          | +  +  +  +  +  +  +  +  +  +  M  +  +  +  M  +  +  +  +  +  +  +  +  M  +|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage, Focal                    |                                                                          |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                1                                                         |             
      Mineralization, Focal                | 1                 1     1  1  2     1  1  1  1     1     1     2  2      |             
      Nephropathy                          |    1        1     2  3  1  2  1  1  1  2  1  1     1  1  2  2  2  1  2  2|             
      Pigmentation, Focal                  |    2     2  1  1  2     1  2  1  1  1  2  1        1  2  1  2  2  2  3   |             
                                            __________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page   9                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 4| 6| 6| 6|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 9| 1| 1| 1|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|                                            |      A     |
    0 PPM                                  | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Esophagus                               |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Colon                  |                                                                          |  49        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Rectum                 |                                                                          |  48        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Cecum                  |                                                                          |  48        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Duodenum               |                                                                          |  48        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Jejunum                |                                                                          |  48        |
      Mineralization, Focal                |                                                                          |      1  1.0|
                                            __________________________________________________________________________|____________|
   Intestine Small, Ileum                  |                                                                          |  48        |
                                            __________________________________________________________________________|____________|
   Liver                                   |                                                                          |  50        |
      Angiectasis, Focal                   |                                                                          |      3  2.0|
      Basophilic Focus                     |                                                                          |     38     |
      Clear Cell Focus                     |                                                                          |     11     |
      Congestion                           |                                                                          |      2  2.5|
      Degeneration, Cystic, Focal          |                                                                          |      2  1.5|
      Developmental Malformation           |                                                                          |      1     |
      Eosinophilic Focus                   |                                                                          |      2     |
      Fatty Change, Diffuse                |                                                                          |      4  3.5|
      Fatty Change, Focal                  |                                                                          |     14  1.6|
      Hematopoietic Cell Proliferation     |                                                                          |      3  1.3|
      Hepatodiaphragmatic Nodule           |                                                                          |      4     |
      Hyperplasia, Focal                   |                                                                          |      4  2.3|
      Inflammation, Chronic, Focal         |                                                                          |     11  1.5|
      Inflammation, Focal, Granulomatous   |                                                                          |      1  3.0|
      Mixed Cell Focus                     |                                                                          |      2     |
      Bile Duct, Hyperplasia, Focal        |                                                                          |      3  1.7|
                                            __________________________________________________________________________|____________|
   Mesentery                               |                                                                          |   8        |
      Fat, Necrosis, Focal                 |                                                                          |      8  2.9|
                                            __________________________________________________________________________|____________|
   Pancreas                                |                                                                          |  50        |
      Acinus, Atrophy, Diffuse             |                                                                          |      1  4.0|
      Acinus, Atrophy, Focal               |                                                                          |     15  1.7|
                                            __________________________________________________________________________|____________|
   Pharynx                                 |                                                                          |   1        |
                                            __________________________________________________________________________|____________|
   Salivary Glands                         |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Stomach, Forestomach                    |                                                                          |  50        |
      Acanthosis, Focal                    |                                                                          |      2  2.5|
      Hyperplasia, Basal Cell, Focal       |                                                                          |      1  2.0|
      Inflammation, Acute, Focal           |                                                                          |      2  2.5|
      Inflammation, Chronic, Focal         |                                                                          |      1  3.0|
      Ulcer, Focal                         |                                                                          |      2  3.0|
      Ulcer                                |                                                                          |      1  2.0|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  10                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 4| 6| 6| 6|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 9| 1| 1| 1|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|                                            |      A     |
    0 PPM                                  | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Stomach, Glandular                      |                                                                          |  50        |
      Erosion, Focal                       |                                                                          |      1  1.0|
      Inflammation, Chronic Active, Focal  |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Heart                                   |                                                                          |  50        |
      Cardiomyopathy                       |                                                                          |     22  1.5|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Adrenal Cortex                          |                                                                          |  50        |
      Angiectasis, Focal                   |                                                                          |      2  2.0|
      Hyperplasia, Focal                   |                                                                          |      4  2.3|
      Hypertrophy, Focal                   |                                                                          |      2  1.5|
      Vacuolization Cytoplasmic, Focal     |                                                                          |     18  1.8|
                                            __________________________________________________________________________|____________|
   Adrenal Medulla                         |                                                                          |  49        |
      Hyperplasia                          |                                                                          |      7  2.3|
                                            __________________________________________________________________________|____________|
   Islets, Pancreatic                      |                                                                          |  50        |
      Hyperplasia, Focal                   |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Parathyroid Gland                       |                                                                          |  45        |
                                            __________________________________________________________________________|____________|
   Pituitary Gland                         |                                                                          |  50        |
      Pars Distalis, Angiectasis, Focal    |                                                                          |      1  2.0|
      Pars Distalis, Cyst                  |                                                                          |     10  2.4|
      Pars Distalis, Hyperplasia, Focal    |                                                                          |     13  1.8|
                                            __________________________________________________________________________|____________|
   Thyroid Gland                           |                                                                          |  50        |
      Inflammation                         |                                                                          |      1  3.0|
      C-Cell, Hyperplasia, Diffuse         |                                                                          |      1  2.0|
      C-Cell, Hyperplasia, Focal           |                                                                          |     10  1.4|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Clitoral Gland                          |                                                                          |  47        |
      Inflammation, Chronic                |                                                                          |      2  1.5|
      Inflammation, Suppurative            |                                                                          |      3  2.7|
      Duct, Dilatation                     |                                                                          |      8  2.5|
                                            __________________________________________________________________________|____________|
   Ovary                                   |                                                                          |  50        |
      Cyst                                 |                                                                          |      3  2.3|
                                            __________________________________________________________________________|____________|
   Uterus                                  |                                                                          |  50        |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  11                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 4| 6| 6| 6|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 9| 1| 1| 1|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|                                            |      A     |
    0 PPM                                  | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 GENITAL SYSTEM - cont                     |                                                                          |            |
                                           |                                                                          |            |
      Cyst                                 |                                                                          |      5  1.6|
      Cyst, Multiple                       |                                                                          |      2  3.0|
      Dilatation                           |                                                                          |      3  1.7|
      Hypoplasia                           |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Vagina                                  |                                                                          |   1        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Bone Marrow                             |                                                                          |  49        |
      Atrophy                              |                                                                          |      7  2.1|
                                            __________________________________________________________________________|____________|
   Lymph Node                              |                                                                          |   7        |
      Mediastinal, Hemorrhage              |                                                                          |      1  2.0|
      Renal, Hemorrhage                    |                                                                          |      2  2.0|
                                            __________________________________________________________________________|____________|
   Lymph Node, Mandibular                  |                                                                          |  49        |
      Hemorrhage                           |                                                                          |      3  2.0|
      Hyperplasia, Lymphoid                |                                                                          |      1  2.0|
      Hyperplasia, Plasma Cell             |                                                                          |      3  1.7|
                                            __________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  |                                                                          |  50        |
      Hemorrhage                           |                                                                          |      6  2.0|
                                            __________________________________________________________________________|____________|
   Spleen                                  |                                                                          |  50        |
      Hematopoietic Cell Proliferation     |                                                                          |      1  4.0|
                                            __________________________________________________________________________|____________|
   Thymus                                  |                                                                          |  48        |
      Hemorrhage                           |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Mammary Gland                           |                                                                          |  50        |
      Degeneration, Focal                  |                                                                          |      1  2.0|
      Galactocele                          |                                                                          |     13  2.2|
      Hyperplasia, Focal                   |                                                                          |      1  2.0|
      Inflammation, Acute, Focal           |                                                                          |      1  2.0|
      Inflammation, Chronic, Focal         |                                                                          |      1  3.0|
      Duct, Dilatation                     |                                                                          |      2  2.0|
                                            __________________________________________________________________________|____________|
   Skin                                    |                                                                          |  50        |
      Cyst Epithelial Inclusion            |                                                                          |      1     |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Bone                                    |                                                                          |  50        |
      Hyperostosis                         |                                                                          |      5  3.0|
      Inflammation, Acute                  |                                                                          |      1  2.0|
      Femur, Osteoporosis                  |                                                                          |      1  4.0|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  12                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 4| 6| 6| 6|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 9| 1| 1| 1|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|                                            |      A     |
    0 PPM                                  | 9| 9| 9| 9| 9| 0| 0| 0| 0| 0|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 MUSCULOSKELETAL SYSTEM - cont             |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Skeletal Muscle                         |                                                                          |   1        |
      Diaphragm, Ectopic Tissue            |                                                                          |      1     |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Brain                                   |                                                                          |  50        |
      Hydrocephalus                        |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Lung                                    |                                                                          |  50        |
      Congestion                           |                                                                          |      1  3.0|
      Hemorrhage, Focal                    |                                                                          |      1  1.0|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Histiocyte                       |                                                                          |     10  1.1|
      Inflammation, Focal                  |                                                                          |      9  1.3|
      Alveolar Epithelium, Hyperplasia,    |                                                                          |            |
           Focal                           |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Nose                                    |                                                                          |  50        |
      Fungus                               |                                                                          |      1  3.0|
      Hemorrhage                           |                                                                          |      1  2.0|
      Inflammation, Acute                  |                                                                          |      2  2.0|
      Inflammation, Chronic Active         |                                                                          |      6  2.3|
      Inflammation, Subacute               |                                                                          |      2  2.5|
                                            __________________________________________________________________________|____________|
   Trachea                                 |                                                                          |  50        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Eye                                     |                                                                          |   2        |
      Phthisis Bulbi                       |                                                                          |      1  4.0|
      Retina, Atrophy                      |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Zymbal's Gland                          |                                                                          |  45        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Kidney                                  |                                                                          |  50        |
      Hemorrhage, Focal                    |                                                                          |      1  1.0|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                                                                          |      1  1.0|
      Mineralization, Focal                |                                                                          |     32  1.3|
      Nephropathy                          |                                                                          |     39  1.6|
      Pigmentation, Focal                  |                                                                          |     34  1.5|
                                            __________________________________________________________________________|____________|
   Urinary Bladder                         |                                                                          |  48        |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  13                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 4| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 7| 7| 7| 7| 7| 7| 6| 4| 7| 6| 6| 5| 7|             
                             DAY ON TEST   | 5| 3| 3| 3| 3| 5| 3| 3| 3| 7| 3| 7| 3| 3| 3| 3| 3| 3| 8| 1| 3| 4| 2| 2| 3|             
                                           | 2| 3| 3| 3| 3| 9| 3| 3| 3| 7| 3| 0| 3| 3| 0| 3| 3| 3| 1| 9| 3| 9| 1| 3| 3|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|             
    1000 PPM                               | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Colon                  | A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Rectum                 | A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Cecum                  | A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Duodenum               | A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Jejunum                | A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Ileum                  | A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                   2                                                      |             
      Basophilic Focus                     |    X  X  X  X     X     X     X     X  X     X  X  X        X           X|             
      Clear Cell Focus                     |       X  X                          X           X                        |             
      Congestion                           |                                                          2               |             
      Developmental Malformation           |                               3                             X            |             
      Eosinophilic Focus                   |    X                                               X                     |             
      Fatty Change, Diffuse                |                3                          4                       3      |             
      Fatty Change, Focal                  |             2                                2        1              2   |             
      Hematopoietic Cell Proliferation     |                                                                          |             
      Hemorrhage, Focal                    |                                                                          |             
      Hepatodiaphragmatic Nodule           |                X                                         X               |             
      Hyperplasia, Focal                   |                                        2                                 |             
      Inflammation, Chronic, Focal         |             1        2                                      1           1|             
      Inflammation, Focal, Granulomatous   |                                                                      2   |             
      Mixed Cell Focus                     |                                                                          |             
      Necrosis, Focal                      |                                              1              2            |             
      Bile Duct, Hyperplasia, Focal        |                                                                          |             
      Vein, Dilatation                     |                                                                          |             
                                            __________________________________________________________________________|             
   Mesentery                               | +                                               +              +         |             
      Fat, Necrosis, Focal                 |                                                 3                        |             
                                            __________________________________________________________________________|             
   Pancreas                                | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acinus, Atrophy, Focal               |       2  2                             1                          1      |             
                                            __________________________________________________________________________|             
   Pharynx                                 |                   +                                                      |             
                                            __________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +|             
      Acanthosis, Focal                    |                                                                          |             
      Hyperplasia, Basal Cell, Focal       |                                                                          |             
      Inflammation, Chronic, Focal         |                                                                          |             
      Ulcer, Focal                         |                            2                                             |             
      Ulcer                                | 2                                                                        |             
                                            __________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       |    1     1  1  1     2  2              2     2  1                    1   |             
      Atrium, Thrombosis                   |                3                          X                              |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  14                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 4| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 7| 7| 7| 7| 7| 7| 6| 4| 7| 6| 6| 5| 7|             
                             DAY ON TEST   | 5| 3| 3| 3| 3| 5| 3| 3| 3| 7| 3| 7| 3| 3| 3| 3| 3| 3| 8| 1| 3| 4| 2| 2| 3|             
                                           | 2| 3| 3| 3| 3| 9| 3| 3| 3| 7| 3| 0| 3| 3| 0| 3| 3| 3| 1| 9| 3| 9| 1| 3| 3|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|             
    1000 PPM                               | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM - cont              |                                                                          |             
                                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |       2                                2        2                       2|             
      Cyst                                 |                                                                          |             
      Degeneration, Cystic, Focal          |    1                                                                     |             
      Hyperplasia, Focal                   |                                                                          |             
      Hypertrophy, Focal                   |                                                             1            |             
      Vacuolization Cytoplasmic, Diffuse   |                            2                                             |             
      Vacuolization Cytoplasmic, Focal     |       1        2  2     3                 2        1        1  2  2      |             
                                            __________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration                         |                                                                          |             
      Hyperplasia                          |                      2           2     2                             1   |             
      Bilateral, Hyperplasia               |                                                    3                     |             
                                            __________________________________________________________________________|             
   Islets, Pancreatic                      | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |                                                                          |             
                                            __________________________________________________________________________|             
   Parathyroid Gland                       | +  M  M  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +|             
      Pars Distalis, Angiectasis           |    3                                                                     |             
      Pars Distalis, Cyst                  |    3     3           2     2     2                                2      |             
      Pars Distalis, Hyperplasia, Focal    |    2     2     1     3                          3                 3     2|             
                                            __________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                                  2                                       |             
      C-Cell, Hyperplasia, Focal           |                         3                                                |             
      Follicle, Dilatation, Focal          |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Tissue NOS                              |                                                                +         |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Duct, Dilatation                     |          3     3  2     2              3           2           3        2|             
                                            __________________________________________________________________________|             
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |    2     3                       3                                   X   |             
                                            __________________________________________________________________________|             
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                      1                    3     1                        |             
      Cyst, Multiple                       |                                  2           2                           |             
      Dilatation                           |                                           2  2                           |             
                                            __________________________________________________________________________|             
   Vagina                                  |                                                                +         |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |                                     2  1                                 |             
      Hyperplasia                          |                                                                2         |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  15                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 4| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 7| 7| 7| 7| 7| 7| 6| 4| 7| 6| 6| 5| 7|             
                             DAY ON TEST   | 5| 3| 3| 3| 3| 5| 3| 3| 3| 7| 3| 7| 3| 3| 3| 3| 3| 3| 8| 1| 3| 4| 2| 2| 3|             
                                           | 2| 3| 3| 3| 3| 9| 3| 3| 3| 7| 3| 0| 3| 3| 0| 3| 3| 3| 1| 9| 3| 9| 1| 3| 3|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|             
    1000 PPM                               | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lymph Node                              |                                  +        +           +                  |             
      Iliac, Hyperplasia, Plasma Cell      |                                                                          |             
      Mediastinal, Hemorrhage              |                                                                          |             
      Mediastinal, Infiltration Cellular,  |                                                                          |             
           Histiocyte                      |                                                                          |             
      Renal, Hemorrhage                    |                                                                          |             
                                            __________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                             2            |             
      Hemorrhage                           |                                                                      1   |             
      Hyperplasia, Plasma Cell             |                                                                          |             
                                            __________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |             2     1                                                      |             
      Hyperplasia, Plasma Cell             |                                                                          |             
                                            __________________________________________________________________________|             
   Spleen                                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic, Focal         |                                                                          |             
                                            __________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +|             
      Cyst, Multiple                       |                                                    3                     |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Galactocele                          |    2                    1     3     2  3  2        3  2     3            |             
      Hyperplasia, Focal                   |                                                                          |             
      Inflammation, Acute, Focal           |                2                                                         |             
      Inflammation, Chronic, Focal         |                                                                          |             
                                            __________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperostosis                         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage, Focal                    |                                                                          |             
      Hydrocephalus                        |                                                             2  2         |             
      Inflammation, Focal                  |                                                             3            |             
      Meninges, Angiectasis, Focal         |                   3                                                      |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage, Focal                    | 2                                                                       1|             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                                                                          |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Histiocyte                       |    1  1     1                                                  1        1|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  16                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 4| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 7| 7| 7| 7| 7| 7| 6| 4| 7| 6| 6| 5| 7|             
                             DAY ON TEST   | 5| 3| 3| 3| 3| 5| 3| 3| 3| 7| 3| 7| 3| 3| 3| 3| 3| 3| 8| 1| 3| 4| 2| 2| 3|             
                                           | 2| 3| 3| 3| 3| 9| 3| 3| 3| 7| 3| 0| 3| 3| 0| 3| 3| 3| 1| 9| 3| 9| 1| 3| 3|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|             
    1000 PPM                               | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
                                           |                                                                          |             
      Inflammation, Focal                  |             2        1                                   1               |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                                                                          |             
                                            __________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |                                                                          |             
      Fungus                               |                                                                          |             
      Inflammation, Acute                  |                            3  2     1                                    |             
      Inflammation, Chronic Active         |                      2           3                 2           3         |             
      Inflammation, Subacute               |    1                                         2                       2   |             
                                            __________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Eye                                     |    +              +                                                      |             
      Hemorrhage                           |    2                                                                     |             
      Bilateral, Lens, Cataract            |                                                                          |             
      Bilateral, Retina, Atrophy           |                                                                          |             
      Lens, Cataract                       |    3                                                                     |             
      Retina, Atrophy                      |    4                                                                     |             
                                            __________________________________________________________________________|             
   Harderian Gland                         |                                                                   +      |             
                                            __________________________________________________________________________|             
   Zymbal's Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  M  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
      Mineralization, Focal                | 2  1  2     2     1  1  1           1           1        2        2      |             
      Nephropathy                          |    2  1  2  1     2  2  2  1        2     1     1  2  2     2  2     3  1|             
      Pigmentation, Focal                  |    1  1        2  1              2        3     1  1  1           3  1   |             
      Bilateral, Hydronephrosis            |                                                                3         |             
                                            __________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Mucosa, Hyperplasia                  |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  17                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 6| 7| 4| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 5| 5|             
                             DAY ON TEST   | 3| 3| 2| 3| 5| 3| 3| 3| 3| 3| 9| 2| 9| 1| 3| 8| 3| 5| 3| 3| 3| 8| 2| 7| 0|             
                                           | 3| 3| 1| 3| 2| 3| 3| 3| 3| 3| 9| 6| 4| 3| 3| 4| 3| 9| 3| 3| 3| 4| 4| 2| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7|             
    1000 PPM                               | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  A  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                                                                          |             
      Basophilic Focus                     | X  X     X     X     X  X  X           X  X     X     X  X           X  X|             
      Clear Cell Focus                     |                                                                          |             
      Congestion                           |                                                                          |             
      Developmental Malformation           | X        X                                                  2            |             
      Eosinophilic Focus                   |                                                                          |             
      Fatty Change, Diffuse                |       3                                            4                     |             
      Fatty Change, Focal                  |                         1     1           1  2        1        3  3      |             
      Hematopoietic Cell Proliferation     |                                        1                                 |             
      Hemorrhage, Focal                    |                            3                                             |             
      Hepatodiaphragmatic Nodule           |                                              X                       X   |             
      Hyperplasia, Focal                   |                                                                          |             
      Inflammation, Chronic, Focal         |                         1  2           1        2           2            |             
      Inflammation, Focal, Granulomatous   |                                              3                           |             
      Mixed Cell Focus                     |          X                                X                              |             
      Necrosis, Focal                      |                                                                          |             
      Bile Duct, Hyperplasia, Focal        |                                  2                                       |             
      Vein, Dilatation                     |                                              2                           |             
                                            __________________________________________________________________________|             
   Mesentery                               |             +           +                                +               |             
      Fat, Necrosis, Focal                 |                         2                                2               |             
                                            __________________________________________________________________________|             
   Pancreas                                | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acinus, Atrophy, Focal               | 2  2                                                  2  3  1            |             
                                            __________________________________________________________________________|             
   Pharynx                                 |                                                                          |             
                                            __________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acanthosis, Focal                    |                                              3     2                     |             
      Hyperplasia, Basal Cell, Focal       |                   1                                                      |             
      Inflammation, Chronic, Focal         |                                              4                           |             
      Ulcer, Focal                         |                                              4                           |             
      Ulcer                                |                                                                          |             
                                            __________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 1  1     1              1     2           1           1  1  1        1   |             
      Atrium, Thrombosis                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  18                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 6| 7| 4| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 5| 5|             
                             DAY ON TEST   | 3| 3| 2| 3| 5| 3| 3| 3| 3| 3| 9| 2| 9| 1| 3| 8| 3| 5| 3| 3| 3| 8| 2| 7| 0|             
                                           | 3| 3| 1| 3| 2| 3| 3| 3| 3| 3| 9| 6| 4| 3| 3| 4| 3| 9| 3| 3| 3| 4| 4| 2| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7|             
    1000 PPM                               | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM - cont              |                                                                          |             
                                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                         2                                                |             
      Cyst                                 |                                              2                           |             
      Degeneration, Cystic, Focal          |                                  3                                       |             
      Hyperplasia, Focal                   |                      1                 1                 3               |             
      Hypertrophy, Focal                   |                            2                    1                        |             
      Vacuolization Cytoplasmic, Diffuse   |                                                    3                     |             
      Vacuolization Cytoplasmic, Focal     |    3     3        3        2  2  3  3  1  1     1              2  3      |             
                                            __________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration                         |                                                                   4      |             
      Hyperplasia                          |                   1                                                      |             
      Bilateral, Hyperplasia               |                                  2                                       |             
                                            __________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |    3                                                                     |             
                                            __________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  M  +  +  +  +  M  +  +  +  +  +  +  +  +  +  M  M  +  +  +  +|             
                                            __________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +|             
      Pars Distalis, Angiectasis           |          3                                                               |             
      Pars Distalis, Cyst                  |          3        2                                            3        X|             
      Pars Distalis, Hyperplasia, Focal    |                         3                                               2|             
                                            __________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation                         |                                                                          |             
      C-Cell, Hyperplasia, Focal           |       1        1                                               1         |             
      Follicle, Dilatation, Focal          |                                                    2                     |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Tissue NOS                              |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  M  +  +|             
      Duct, Dilatation                     |          3                                2  3              2            |             
                                            __________________________________________________________________________|             
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                            3        X        2           2        2      |             
                                            __________________________________________________________________________|             
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |       1                    1              1                          1   |             
      Cyst, Multiple                       |                   3                                                      |             
      Dilatation                           |                   3  1  2                                                |             
                                            __________________________________________________________________________|             
   Vagina                                  |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |    2     2                                                               |             
      Hyperplasia                          |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  19                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 6| 7| 4| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 5| 5|             
                             DAY ON TEST   | 3| 3| 2| 3| 5| 3| 3| 3| 3| 3| 9| 2| 9| 1| 3| 8| 3| 5| 3| 3| 3| 8| 2| 7| 0|             
                                           | 3| 3| 1| 3| 2| 3| 3| 3| 3| 3| 9| 6| 4| 3| 3| 4| 3| 9| 3| 3| 3| 4| 4| 2| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7|             
    1000 PPM                               | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lymph Node                              |                   +  +           +     +     +     +           +  +     +|             
      Iliac, Hyperplasia, Plasma Cell      |                                                                         2|             
      Mediastinal, Hemorrhage              |                      2                 3                                 |             
      Mediastinal, Infiltration Cellular,  |                                                                          |             
           Histiocyte                      |                      2                                                   |             
      Renal, Hemorrhage                    |                                              2                           |             
                                            __________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                1                                      2                  |             
      Hemorrhage                           |                                                                          |             
      Hyperplasia, Plasma Cell             |                                                       3                  |             
                                            __________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                      2              2                                    |             
      Hyperplasia, Plasma Cell             |                                                                3         |             
                                            __________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic, Focal         |                                                 2                        |             
                                            __________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst, Multiple                       |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Galactocele                          | 2  2           3           3                                   2         |             
      Hyperplasia, Focal                   |                2                                                         |             
      Inflammation, Acute, Focal           |                                                                          |             
      Inflammation, Chronic, Focal         |                3                             2                           |             
                                            __________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperostosis                         |                3                                         3               |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage, Focal                    |                                              1                           |             
      Hydrocephalus                        |                            1  3        2     3                           |             
      Inflammation, Focal                  |                                                                          |             
      Meninges, Angiectasis, Focal         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage, Focal                    |                                        1                                 |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                                        2                                 |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Histiocyte                       |                                                 1                       1|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  20                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 6| 7| 4| 7| 7| 6| 7| 6| 7| 7| 7| 6| 6| 5| 5|             
                             DAY ON TEST   | 3| 3| 2| 3| 5| 3| 3| 3| 3| 3| 9| 2| 9| 1| 3| 8| 3| 5| 3| 3| 3| 8| 2| 7| 0|             
                                           | 3| 3| 1| 3| 2| 3| 3| 3| 3| 3| 9| 6| 4| 3| 3| 4| 3| 9| 3| 3| 3| 4| 4| 2| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7|             
    1000 PPM                               | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
                                           |                                                                          |             
      Inflammation, Focal                  |                         1                    2        1                  |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                                        1                                 |             
                                            __________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |                                                 2                        |             
      Fungus                               |                                  3                                       |             
      Inflammation, Acute                  |                                                                          |             
      Inflammation, Chronic Active         |                               2  3                                       |             
      Inflammation, Subacute               |                2        2                                                |             
                                            __________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Eye                                     |                +           +                                             |             
      Hemorrhage                           |                                                                          |             
      Bilateral, Lens, Cataract            |                2           3                                             |             
      Bilateral, Retina, Atrophy           |                3           2                                             |             
      Lens, Cataract                       |                                                                          |             
      Retina, Atrophy                      |                                                                          |             
                                            __________________________________________________________________________|             
   Harderian Gland                         |                                                                          |             
                                            __________________________________________________________________________|             
   Zymbal's Gland                          | +  +  +  M  +  M  +  +  +  M  +  +  +  +  +  +  M  +  +  M  M  M  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                  4                                       |             
      Mineralization, Focal                |       1  1     1  1  1  1  1           2  1        2        1     2  2  1|             
      Nephropathy                          | 2  2  1  1     3  1  1  1  2  2     2     1  3  1  2  1  1  2  2  2     2|             
      Pigmentation, Focal                  | 1  3  3  1                 1           1  1  2     2        1  2  2     1|             
      Bilateral, Hydronephrosis            |                                                                          |             
                                            __________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Mucosa, Hyperplasia                  |    2                                                                     |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  21                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 3|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 1| 1| 1| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                                            |      A     |
    1000 PPM                               | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Esophagus                               |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Colon                  |                                                                          |  47        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Rectum                 |                                                                          |  47        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Cecum                  |                                                                          |  47        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Duodenum               |                                                                          |  48        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Jejunum                |                                                                          |  45        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Ileum                  |                                                                          |  46        |
                                            __________________________________________________________________________|____________|
   Liver                                   |                                                                          |  50        |
      Angiectasis, Focal                   |                                                                          |      1  2.0|
      Basophilic Focus                     |                                                                          |     28     |
      Clear Cell Focus                     |                                                                          |      4     |
      Congestion                           |                                                                          |      1  2.0|
      Developmental Malformation           |                                                                          |      5  2.5|
      Eosinophilic Focus                   |                                                                          |      2     |
      Fatty Change, Diffuse                |                                                                          |      5  3.4|
      Fatty Change, Focal                  |                                                                          |     11  1.7|
      Hematopoietic Cell Proliferation     |                                                                          |      1  1.0|
      Hemorrhage, Focal                    |                                                                          |      1  3.0|
      Hepatodiaphragmatic Nodule           |                                                                          |      4     |
      Hyperplasia, Focal                   |                                                                          |      1  2.0|
      Inflammation, Chronic, Focal         |                                                                          |      9  1.4|
      Inflammation, Focal, Granulomatous   |                                                                          |      2  2.5|
      Mixed Cell Focus                     |                                                                          |      2     |
      Necrosis, Focal                      |                                                                          |      2  1.5|
      Bile Duct, Hyperplasia, Focal        |                                                                          |      1  2.0|
      Vein, Dilatation                     |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Mesentery                               |                                                                          |   6        |
      Fat, Necrosis, Focal                 |                                                                          |      3  2.3|
                                            __________________________________________________________________________|____________|
   Pancreas                                |                                                                          |  48        |
      Acinus, Atrophy, Focal               |                                                                          |      9  1.8|
                                            __________________________________________________________________________|____________|
   Pharynx                                 |                                                                          |   1        |
                                            __________________________________________________________________________|____________|
   Salivary Glands                         |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Stomach, Forestomach                    |                                                                          |  49        |
      Acanthosis, Focal                    |                                                                          |      2  2.5|
      Hyperplasia, Basal Cell, Focal       |                                                                          |      1  1.0|
      Inflammation, Chronic, Focal         |                                                                          |      1  4.0|
      Ulcer, Focal                         |                                                                          |      2  3.0|
      Ulcer                                |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Stomach, Glandular                      |                                                                          |  48        |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  22                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 3|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 1| 1| 1| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                                            |      A     |
    1000 PPM                               | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
                                           |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Heart                                   |                                                                          |  50        |
      Cardiomyopathy                       |                                                                          |     20  1.3|
      Atrium, Thrombosis                   |                                                                          |      2  3.0|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Adrenal Cortex                          |                                                                          |  50        |
      Angiectasis, Focal                   |                                                                          |      5  2.0|
      Cyst                                 |                                                                          |      1  2.0|
      Degeneration, Cystic, Focal          |                                                                          |      2  2.0|
      Hyperplasia, Focal                   |                                                                          |      3  1.7|
      Hypertrophy, Focal                   |                                                                          |      3  1.3|
      Vacuolization Cytoplasmic, Diffuse   |                                                                          |      2  2.5|
      Vacuolization Cytoplasmic, Focal     |                                                                          |     21  2.0|
                                            __________________________________________________________________________|____________|
   Adrenal Medulla                         |                                                                          |  50        |
      Degeneration                         |                                                                          |      1  4.0|
      Hyperplasia                          |                                                                          |      5  1.6|
      Bilateral, Hyperplasia               |                                                                          |      2  2.5|
                                            __________________________________________________________________________|____________|
   Islets, Pancreatic                      |                                                                          |  48        |
      Hyperplasia, Focal                   |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Parathyroid Gland                       |                                                                          |  43        |
                                            __________________________________________________________________________|____________|
   Pituitary Gland                         |                                                                          |  47        |
      Pars Distalis, Angiectasis           |                                                                          |      2  3.0|
      Pars Distalis, Cyst                  |                                                                          |     10  2.4|
      Pars Distalis, Hyperplasia, Focal    |                                                                          |      9  2.3|
                                            __________________________________________________________________________|____________|
   Thyroid Gland                           |                                                                          |  50        |
      Inflammation                         |                                                                          |      1  2.0|
      C-Cell, Hyperplasia, Focal           |                                                                          |      4  1.5|
      Follicle, Dilatation, Focal          |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Tissue NOS                              |                                                                          |   1        |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Clitoral Gland                          |                                                                          |  48        |
      Duct, Dilatation                     |                                                                          |     12  2.5|
                                            __________________________________________________________________________|____________|
   Ovary                                   |                                                                          |  50        |
      Cyst                                 |                                                                          |      9  2.4|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  23                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 3|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 1| 1| 1| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                                            |      A     |
    1000 PPM                               | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 GENITAL SYSTEM - cont                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Uterus                                  |                                                                          |  50        |
      Cyst                                 |                                                                          |      7  1.3|
      Cyst, Multiple                       |                                                                          |      3  2.3|
      Dilatation                           |                                                                          |      5  2.0|
                                            __________________________________________________________________________|____________|
   Vagina                                  |                                                                          |   1        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Bone Marrow                             |                                                                          |  49        |
      Atrophy                              |                                                                          |      4  1.8|
      Hyperplasia                          |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Lymph Node                              |                                                                          |  12        |
      Iliac, Hyperplasia, Plasma Cell      |                                                                          |      1  2.0|
      Mediastinal, Hemorrhage              |                                                                          |      2  2.5|
      Mediastinal, Infiltration Cellular,  |                                                                          |            |
           Histiocyte                      |                                                                          |      1  2.0|
      Renal, Hemorrhage                    |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Lymph Node, Mandibular                  |                                                                          |  50        |
      Cyst                                 |                                                                          |      3  1.7|
      Hemorrhage                           |                                                                          |      1  1.0|
      Hyperplasia, Plasma Cell             |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  |                                                                          |  50        |
      Hemorrhage                           |                                                                          |      4  1.8|
      Hyperplasia, Plasma Cell             |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Spleen                                  |                                                                          |  49        |
      Inflammation, Chronic, Focal         |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Thymus                                  |                                                                          |  49        |
      Cyst, Multiple                       |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Mammary Gland                           |                                                                          |  50        |
      Galactocele                          |                                                                          |     14  2.4|
      Hyperplasia, Focal                   |                                                                          |      1  2.0|
      Inflammation, Acute, Focal           |                                                                          |      1  2.0|
      Inflammation, Chronic, Focal         |                                                                          |      2  2.5|
                                            __________________________________________________________________________|____________|
   Skin                                    |                                                                          |  50        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Bone                                    |                                                                          |  50        |
      Hyperostosis                         |                                                                          |      2  3.0|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  24                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 3|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 1| 1| 1| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                                            |      A     |
    1000 PPM                               | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 MUSCULOSKELETAL SYSTEM - cont             |                                                                          |            |
                                           |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Brain                                   |                                                                          |  50        |
      Hemorrhage, Focal                    |                                                                          |      1  1.0|
      Hydrocephalus                        |                                                                          |      6  2.2|
      Inflammation, Focal                  |                                                                          |      1  3.0|
      Meninges, Angiectasis, Focal         |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Lung                                    |                                                                          |  50        |
      Hemorrhage, Focal                    |                                                                          |      3  1.3|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                                                                          |      1  2.0|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Histiocyte                       |                                                                          |      7  1.0|
      Inflammation, Focal                  |                                                                          |      6  1.3|
      Alveolar Epithelium, Hyperplasia,    |                                                                          |            |
           Focal                           |                                                                          |      1  1.0|
                                            __________________________________________________________________________|____________|
   Nose                                    |                                                                          |  50        |
      Foreign Body                         |                                                                          |      1  2.0|
      Fungus                               |                                                                          |      1  3.0|
      Inflammation, Acute                  |                                                                          |      3  2.0|
      Inflammation, Chronic Active         |                                                                          |      6  2.5|
      Inflammation, Subacute               |                                                                          |      5  1.8|
                                            __________________________________________________________________________|____________|
   Trachea                                 |                                                                          |  50        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Eye                                     |                                                                          |   4        |
      Hemorrhage                           |                                                                          |      1  2.0|
      Bilateral, Lens, Cataract            |                                                                          |      2  2.5|
      Bilateral, Retina, Atrophy           |                                                                          |      2  2.5|
      Lens, Cataract                       |                                                                          |      1  3.0|
      Retina, Atrophy                      |                                                                          |      1  4.0|
                                            __________________________________________________________________________|____________|
   Harderian Gland                         |                                                                          |   1        |
                                            __________________________________________________________________________|____________|
   Zymbal's Gland                          |                                                                          |  41        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Kidney                                  |                                                                          |  50        |
      Cyst                                 |                                                                          |      1  4.0|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  25                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 3|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 1| 1| 1| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                                            |      A     |
    1000 PPM                               | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 URINARY SYSTEM - cont                     |                                                                          |            |
                                           |                                                                          |            |
      Mineralization, Focal                |                                                                          |     25  1.4|
      Nephropathy                          |                                                                          |     38  1.7|
      Pigmentation, Focal                  |                                                                          |     24  1.6|
      Bilateral, Hydronephrosis            |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Urinary Bladder                         |                                                                          |  48        |
      Mucosa, Hyperplasia                  |                                                                          |      1  2.0|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  26                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 7| 5| 7| 4| 5| 7| 6| 7| 7| 7| 5| 7| 7| 6| 7| 7| 7| 7| 5| 7| 6| 7|             
                             DAY ON TEST   | 3| 3| 6| 3| 4| 3| 2| 0| 0| 8| 3| 3| 3| 8| 3| 3| 7| 3| 3| 3| 3| 3| 3| 1| 3|             
                                           | 0| 0| 0| 0| 0| 0| 8| 6| 6| 8| 0| 0| 0| 3| 0| 0| 4| 0| 0| 0| 0| 2| 0| 7| 0|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
    3000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  I|             
                                            __________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     | X  X  X  X     X     X     X  X  X  X  X  X  X  X  X  X  X  X     X     X|             
      Clear Cell Focus                     |                X              X     X              X  X  X  X            |             
      Congestion                           |                                                                          |             
      Cyst                                 |                                                                          |             
      Developmental Malformation           |                   X                                                      |             
      Eosinophilic Focus                   | X                                                                        |             
      Fatty Change, Diffuse                |                                                                          |             
      Fatty Change, Focal                  | 2     1                    2           2                                 |             
      Hepatodiaphragmatic Nodule           |                                           X           X                  |             
      Hyperplasia, Focal                   |                                                                          |             
      Inflammation, Chronic, Focal         |    2  1                                1                                 |             
      Inflammation, Focal, Granulomatous   |                                                                          |             
      Necrosis, Focal                      |             3                                                            |             
      Bile Duct, Dilatation, Focal         |                                                                         1|             
      Bile Duct, Hyperplasia, Focal        |          2                                                               |             
      Vein, Dilatation                     |                                                                          |             
                                            __________________________________________________________________________|             
   Mesentery                               |             +              +                                             |             
      Fat, Necrosis, Focal                 |                            3                                             |             
                                            __________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acinus, Atrophy, Focal               |                               1     2  2           2  1                  |             
                                            __________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acanthosis, Focal                    |       2                                                                  |             
      Hyperplasia, Basal Cell, Focal       | 1     1                                      1                           |             
      Mineralization, Focal                |             2                                                            |             
      Ulcer, Focal                         |             2                                                            |             
                                            __________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Erosion, Focal                       |                                                                          |             
      Ulcer, Focal                         |                                                                          |             
                                            __________________________________________________________________________|             
   Tongue                                  |                                                                          |             
      Acanthosis, Focal                    |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 2                             1     2     1     2  1  1  2  1           2|             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  27                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 7| 5| 7| 4| 5| 7| 6| 7| 7| 7| 5| 7| 7| 6| 7| 7| 7| 7| 5| 7| 6| 7|             
                             DAY ON TEST   | 3| 3| 6| 3| 4| 3| 2| 0| 0| 8| 3| 3| 3| 8| 3| 3| 7| 3| 3| 3| 3| 3| 3| 1| 3|             
                                           | 0| 0| 0| 0| 0| 0| 8| 6| 6| 8| 0| 0| 0| 3| 0| 0| 4| 0| 0| 0| 0| 2| 0| 7| 0|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
    3000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
                                           |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |          2                       2           2  2        2        2      |             
      Cyst                                 |          2                                                               |             
      Degeneration, Cystic, Focal          |                         2                                                |             
      Hematopoietic Cell Proliferation,    |                                                                          |             
           Focal                           |                      2                                                   |             
      Hemorrhage                           |                            2                                             |             
      Hyperplasia, Focal                   |    2           2                    2                                    |             
      Hypertrophy, Focal                   |                                                                          |             
      Vacuolization Cytoplasmic, Focal     | 3  1  1  2     1        1     1           2     1  3  2                  |             
                                            __________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                        2     3        2                  |             
      Bilateral, Hyperplasia               |                                                                          |             
                                            __________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |                                                                          |             
                                            __________________________________________________________________________|             
   Parathyroid Gland                       | M  +  +  +  +  +  +  +  +  +  +  M  +  M  M  +  +  +  +  +  +  +  +  +  M|             
                                            __________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage, Focal                    |                                                                          |             
      Pars Distalis, Cyst                  |    2                                                                 2   |             
      Pars Distalis, Hyperplasia, Focal    |             2  1           2                          3              2   |             
                                            __________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M|             
      C-Cell, Hyperplasia, Focal           |                      2                    2                              |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                     2                                    |             
      Inflammation, Suppurative            |       3                                                                  |             
      Bilateral, Duct, Dilatation          |                                                                          |             
      Duct, Dilatation                     |                2     2  2        2     2                 2  2        3   |             
                                            __________________________________________________________________________|             
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                                          |             
      Cyst                                 |                2                       2     3           2           3   |             
                                            __________________________________________________________________________|             
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |          2                    2  1           1     1                    1|             
      Dilatation                           |    3     2                    3                                          |             
      Inflammation, Acute                  |                                                                          |             
                                            __________________________________________________________________________|             
   Vagina                                  |                                                          +               |             
      Cyst                                 |                                                          4               |             
      Hyperplasia, Focal, Squamous         |                                                          3               |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  28                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 7| 5| 7| 4| 5| 7| 6| 7| 7| 7| 5| 7| 7| 6| 7| 7| 7| 7| 5| 7| 6| 7|             
                             DAY ON TEST   | 3| 3| 6| 3| 4| 3| 2| 0| 0| 8| 3| 3| 3| 8| 3| 3| 7| 3| 3| 3| 3| 3| 3| 1| 3|             
                                           | 0| 0| 0| 0| 0| 0| 8| 6| 6| 8| 0| 0| 0| 3| 0| 0| 4| 0| 0| 0| 0| 2| 0| 7| 0|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
    3000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
                                           |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |          2                                2           2     2           2|             
                                            __________________________________________________________________________|             
   Lymph Node                              |       +              +                 +                                 |             
      Axillary, Hyperplasia, Plasma Cell   |                      2                                                   |             
      Mediastinal, Hemorrhage              |       2                                1                                 |             
      Renal, Hemorrhage                    |       2                                                                  |             
                                            __________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |       2                    2                                             |             
                                            __________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                          1               |             
      Hyperplasia, Lymphoid                |                   2                                                      |             
                                            __________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hematopoietic Cell Proliferation     |             3        3                                                   |             
                                            __________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Galactocele                          | 2                                2     2           1                     |             
      Duct, Dilatation                     |                                                                2         |             
                                            __________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperostosis                         |    4                          3                                   3      |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage, Focal                    |                                                                          |             
      Hydrocephalus                        |                         3  2                                   3         |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                                          |             
      Hemorrhage, Focal                    |    2                                         1                           |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                                        2                                 |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Histiocyte                       |    2     1           1                                   1              1|             
      Inflammation, Focal                  |    2  2  2                    1                                          |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                                                                          |             
                                            __________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  29                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 7| 5| 7| 4| 5| 7| 6| 7| 7| 7| 5| 7| 7| 6| 7| 7| 7| 7| 5| 7| 6| 7|             
                             DAY ON TEST   | 3| 3| 6| 3| 4| 3| 2| 0| 0| 8| 3| 3| 3| 8| 3| 3| 7| 3| 3| 3| 3| 3| 3| 1| 3|             
                                           | 0| 0| 0| 0| 0| 0| 8| 6| 6| 8| 0| 0| 0| 3| 0| 0| 4| 0| 0| 0| 0| 2| 0| 7| 0|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|             
    3000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
                                           |                                                                          |             
      Fungus                               |                                  3     2        2                        |             
      Hemorrhage                           |                                                                          |             
      Inflammation, Acute                  |                                                 2        1               |             
      Inflammation, Chronic Active         |                                  3     3                                 |             
      Inflammation, Subacute               |                                                                2         |             
      Goblet Cell, Hyperplasia             |    3                                                                     |             
                                            __________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Ear                                     |                                                 +                        |             
                                            __________________________________________________________________________|             
   Eye                                     | +  +     +                                                               |             
      Inflammation, Acute                  |                                                                          |             
      Bilateral, Lens, Cataract            | 2  2     2                                                               |             
      Bilateral, Retina, Atrophy           | 3  3     3                                                               |             
      Lens, Cataract                       |                                                                          |             
      Retina, Atrophy                      |                                                                          |             
                                            __________________________________________________________________________|             
   Zymbal's Gland                          | +  +  M  +  +  M  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                                          |             
      Cyst                                 |                                                                          |             
      Cyst, Multiple                       |                                                                          |             
      Mineralization, Focal                | 1     2  2        1        2  2        2        1     1  1  1        2  1|             
      Nephropathy                          | 3  1  3  3  2  1     1  3  2  2  2  1  1  1  2     3  1     1  3     2  2|             
      Pigmentation, Focal                  | 2     2  2  2  2     2  3  2  1  1        1  1  2           1  2         |             
                                            __________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  30                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 6| 5| 7| 7| 6| 7| 6| 7| 3| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 1| 5| 8| 3| 2| 2| 3| 6| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
                                           | 0| 0| 0| 2| 7| 0| 6| 9| 0| 9| 0| 7| 0| 0| 0| 0| 0| 0| 0| 9| 0| 3| 0| 0| 0|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8|             
    3000 PPM                               | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     | X  X        X  X  X  X  X  X  X     X  X  X  X  X  X  X     X  X  X  X  X|             
      Clear Cell Focus                     |    X                 X  X     X     X  X        X                    X  X|             
      Congestion                           |                            2     3                             2         |             
      Cyst                                 |                                                          X               |             
      Developmental Malformation           |                                                                          |             
      Eosinophilic Focus                   |       X                                X                                 |             
      Fatty Change, Diffuse                |          3                                               3               |             
      Fatty Change, Focal                  |                   3           2     1                             1     2|             
      Hepatodiaphragmatic Nodule           |                                                                          |             
      Hyperplasia, Focal                   |                                                       2                  |             
      Inflammation, Chronic, Focal         |                1        2     1     2                                1   |             
      Inflammation, Focal, Granulomatous   |                      2                                                   |             
      Necrosis, Focal                      |                                                                          |             
      Bile Duct, Dilatation, Focal         |                                                                          |             
      Bile Duct, Hyperplasia, Focal        |                   1                                                      |             
      Vein, Dilatation                     |                                                          3               |             
                                            __________________________________________________________________________|             
   Mesentery                               |          +                                                               |             
      Fat, Necrosis, Focal                 |          3                                                               |             
                                            __________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acinus, Atrophy, Focal               |                      2  1     2                       2                  |             
                                            __________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acanthosis, Focal                    |    1                                                     3               |             
      Hyperplasia, Basal Cell, Focal       | 1                                               1                        |             
      Mineralization, Focal                |                                                                          |             
      Ulcer, Focal                         |                                                          2               |             
                                            __________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Erosion, Focal                       |                                                          2               |             
      Ulcer, Focal                         |                                                          3               |             
                                            __________________________________________________________________________|             
   Tongue                                  |                +                                                         |             
      Acanthosis, Focal                    |                1                                                         |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 2  1              1  2     2  1     1  1     1  1                       2|             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  31                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 6| 5| 7| 7| 6| 7| 6| 7| 3| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 1| 5| 8| 3| 2| 2| 3| 6| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
                                           | 0| 0| 0| 2| 7| 0| 6| 9| 0| 9| 0| 7| 0| 0| 0| 0| 0| 0| 0| 9| 0| 3| 0| 0| 0|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8|             
    3000 PPM                               | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
                                           |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |       2              2     2              2     1                    2  2|             
      Cyst                                 |                                                                          |             
      Degeneration, Cystic, Focal          |                                                                          |             
      Hematopoietic Cell Proliferation,    |                                                                          |             
           Focal                           |                                                                          |             
      Hemorrhage                           |                                                                          |             
      Hyperplasia, Focal                   |                                           1  1                       2  1|             
      Hypertrophy, Focal                   |                               2                                      2   |             
      Vacuolization Cytoplasmic, Focal     |       1  2                                   1        3                 2|             
                                            __________________________________________________________________________|             
   Adrenal Medulla                         | M  M  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                         2     2  1                                       |             
      Bilateral, Hyperplasia               |                      2                                                   |             
                                            __________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |                                                       2                  |             
                                            __________________________________________________________________________|             
   Parathyroid Gland                       | M  M  +  +  +  +  +  +  M  +  +  +  +  M  M  +  +  +  +  +  +  +  M  +  +|             
                                            __________________________________________________________________________|             
   Pituitary Gland                         | +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage, Focal                    |                            2                                             |             
      Pars Distalis, Cyst                  | 2        2              2           2                       3            |             
      Pars Distalis, Hyperplasia, Focal    |                               2     2              2              3      |             
                                            __________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      C-Cell, Hyperplasia, Focal           |    1              1                             1                       3|             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Clitoral Gland                          | +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                                             4        2   |             
      Inflammation, Suppurative            |                                                                          |             
      Bilateral, Duct, Dilatation          |                                        3                                 |             
      Duct, Dilatation                     | 3        2     2           2                       2                 2  2|             
                                            __________________________________________________________________________|             
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                            2                                             |             
      Cyst                                 |                                                                          |             
                                            __________________________________________________________________________|             
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                   1                                      1               |             
      Dilatation                           |             2        2        2                                          |             
      Inflammation, Acute                  |                      1                                                   |             
                                            __________________________________________________________________________|             
   Vagina                                  | +                                                                        |             
      Cyst                                 | 2                                                                        |             
      Hyperplasia, Focal, Squamous         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  32                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 6| 5| 7| 7| 6| 7| 6| 7| 3| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 1| 5| 8| 3| 2| 2| 3| 6| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
                                           | 0| 0| 0| 2| 7| 0| 6| 9| 0| 9| 0| 7| 0| 0| 0| 0| 0| 0| 0| 9| 0| 3| 0| 0| 0|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8|             
    3000 PPM                               | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
                                           |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |       3                       1                       2              2  2|             
                                            __________________________________________________________________________|             
   Lymph Node                              |          +                 +                             +               |             
      Axillary, Hyperplasia, Plasma Cell   |                                                                          |             
      Mediastinal, Hemorrhage              |                            3                                             |             
      Renal, Hemorrhage                    |                                                                          |             
                                            __________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                            3                                             |             
                                            __________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                   2                                               2      |             
      Hyperplasia, Lymphoid                |                                                                          |             
                                            __________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hematopoietic Cell Proliferation     |                                                                          |             
                                            __________________________________________________________________________|             
   Thymus                                  | M  M  +  +  +  +  +  +  +  M  +  +  +  +  +  +  M  M  M  +  M  +  +  +  +|             
      Congestion                           |                                                                3         |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Galactocele                          |                         1              1        2     2     2           2|             
      Duct, Dilatation                     |                                                                          |             
                                            __________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperostosis                         |                3                                            3            |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage, Focal                    |                            2                                   2         |             
      Hydrocephalus                        |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                            3                                   2         |             
      Hemorrhage, Focal                    |                                                                          |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |       1                                                                  |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Histiocyte                       |                               1        1                                 |             
      Inflammation, Focal                  |                                     1     2  2     1           2     1  2|             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                                           2                              |             
                                            __________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  33                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 6| 6| 5| 7| 7| 6| 7| 6| 7| 3| 7| 7| 7| 7| 7| 7| 7| 6| 7| 5| 7| 7| 7|             
                             DAY ON TEST   | 3| 3| 1| 5| 8| 3| 2| 2| 3| 6| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
                                           | 0| 0| 0| 2| 7| 0| 6| 9| 0| 9| 0| 7| 0| 0| 0| 0| 0| 0| 0| 9| 0| 3| 0| 0| 0|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8|             
    3000 PPM                               | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
                                           |                                                                          |             
      Fungus                               |                                  3           3  2                        |             
      Hemorrhage                           |                                     3                 2                  |             
      Inflammation, Acute                  |                                                                          |             
      Inflammation, Chronic Active         | 2        2        2              4           3  2           2            |             
      Inflammation, Subacute               |             2                       1                                    |             
      Goblet Cell, Hyperplasia             |                                                                          |             
                                            __________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Ear                                     |                                                                          |             
                                            __________________________________________________________________________|             
   Eye                                     | +                                +              +                        |             
      Inflammation, Acute                  | 3                                                                        |             
      Bilateral, Lens, Cataract            |                                                                          |             
      Bilateral, Retina, Atrophy           |                                                                          |             
      Lens, Cataract                       |                                                 2                        |             
      Retina, Atrophy                      |                                                 4                        |             
                                            __________________________________________________________________________|             
   Zymbal's Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                            2                                             |             
      Cyst                                 |                                                          3               |             
      Cyst, Multiple                       |                                                                   3      |             
      Mineralization, Focal                |       2     2  1     2  1  1     1  2     1     1  1  1  2  1           2|             
      Nephropathy                          | 1  1  2  1     3  2     1  1  2     3  1  1  2  1  2  2  3  1  1        2|             
      Pigmentation, Focal                  |       2  2  1  1  1  1  1           1  2     1     1     2           1   |             
                                            __________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  34                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8|                                            |      A     |
    3000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Esophagus                               |                                                                          |  49        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Colon                  |                                                                          |  49        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Rectum                 |                                                                          |  49        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Cecum                  |                                                                          |  48        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Duodenum               |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Jejunum                |                                                                          |  49        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Ileum                  |                                                                          |  48        |
                                            __________________________________________________________________________|____________|
   Liver                                   |                                                                          |  50        |
      Basophilic Focus                     |                                                                          |     41     |
      Clear Cell Focus                     |                                                                          |     16     |
      Congestion                           |                                                                          |      3  2.3|
      Cyst                                 |                                                                          |      1     |
      Developmental Malformation           |                                                                          |      1     |
      Eosinophilic Focus                   |                                                                          |      3     |
      Fatty Change, Diffuse                |                                                                          |      2  3.0|
      Fatty Change, Focal                  |                                                                          |      9  1.8|
      Hepatodiaphragmatic Nodule           |                                                                          |      2     |
      Hyperplasia, Focal                   |                                                                          |      1  2.0|
      Inflammation, Chronic, Focal         |                                                                          |      8  1.4|
      Inflammation, Focal, Granulomatous   |                                                                          |      1  2.0|
      Necrosis, Focal                      |                                                                          |      1  3.0|
      Bile Duct, Dilatation, Focal         |                                                                          |      1  1.0|
      Bile Duct, Hyperplasia, Focal        |                                                                          |      2  1.5|
      Vein, Dilatation                     |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Mesentery                               |                                                                          |   3        |
      Fat, Necrosis, Focal                 |                                                                          |      2  3.0|
                                            __________________________________________________________________________|____________|
   Pancreas                                |                                                                          |  50        |
      Acinus, Atrophy, Focal               |                                                                          |      9  1.7|
                                            __________________________________________________________________________|____________|
   Salivary Glands                         |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Stomach, Forestomach                    |                                                                          |  50        |
      Acanthosis, Focal                    |                                                                          |      3  2.0|
      Hyperplasia, Basal Cell, Focal       |                                                                          |      5  1.0|
      Mineralization, Focal                |                                                                          |      1  2.0|
      Ulcer, Focal                         |                                                                          |      2  2.0|
                                            __________________________________________________________________________|____________|
   Stomach, Glandular                      |                                                                          |  50        |
      Erosion, Focal                       |                                                                          |      1  2.0|
      Ulcer, Focal                         |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Tongue                                  |                                                                          |   1        |
      Acanthosis, Focal                    |                                                                          |      1  1.0|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  35                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8|                                            |      A     |
    3000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
                                           |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Heart                                   |                                                                          |  50        |
      Cardiomyopathy                       |                                                                          |     21  1.4|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Adrenal Cortex                          |                                                                          |  50        |
      Angiectasis, Focal                   |                                                                          |     13  1.9|
      Cyst                                 |                                                                          |      1  2.0|
      Degeneration, Cystic, Focal          |                                                                          |      1  2.0|
      Hematopoietic Cell Proliferation,    |                                                                          |            |
           Focal                           |                                                                          |      1  2.0|
      Hemorrhage                           |                                                                          |      1  2.0|
      Hyperplasia, Focal                   |                                                                          |      7  1.6|
      Hypertrophy, Focal                   |                                                                          |      2  2.0|
      Vacuolization Cytoplasmic, Focal     |                                                                          |     16  1.7|
                                            __________________________________________________________________________|____________|
   Adrenal Medulla                         |                                                                          |  47        |
      Hyperplasia                          |                                                                          |      6  2.0|
      Bilateral, Hyperplasia               |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Islets, Pancreatic                      |                                                                          |  50        |
      Hyperplasia, Focal                   |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Parathyroid Gland                       |                                                                          |  39        |
                                            __________________________________________________________________________|____________|
   Pituitary Gland                         |                                                                          |  49        |
      Hemorrhage, Focal                    |                                                                          |      1  2.0|
      Pars Distalis, Cyst                  |                                                                          |      7  2.1|
      Pars Distalis, Hyperplasia, Focal    |                                                                          |      9  2.1|
                                            __________________________________________________________________________|____________|
   Thyroid Gland                           |                                                                          |  49        |
      C-Cell, Hyperplasia, Focal           |                                                                          |      6  1.7|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Clitoral Gland                          |                                                                          |  49        |
      Hyperplasia                          |                                                                          |      3  2.7|
      Inflammation, Suppurative            |                                                                          |      1  3.0|
      Bilateral, Duct, Dilatation          |                                                                          |      1  3.0|
      Duct, Dilatation                     |                                                                          |     15  2.1|
                                            __________________________________________________________________________|____________|
   Ovary                                   |                                                                          |  50        |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  36                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8|                                            |      A     |
    3000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 GENITAL SYSTEM - cont                     |                                                                          |            |
                                           |                                                                          |            |
      Congestion                           |                                                                          |      1  2.0|
      Cyst                                 |                                                                          |      5  2.4|
                                            __________________________________________________________________________|____________|
   Uterus                                  |                                                                          |  50        |
      Cyst                                 |                                                                          |      8  1.3|
      Dilatation                           |                                                                          |      6  2.3|
      Inflammation, Acute                  |                                                                          |      1  1.0|
                                            __________________________________________________________________________|____________|
   Vagina                                  |                                                                          |   2        |
      Cyst                                 |                                                                          |      2  3.0|
      Hyperplasia, Focal, Squamous         |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Bone Marrow                             |                                                                          |  50        |
      Atrophy                              |                                                                          |     10  2.0|
                                            __________________________________________________________________________|____________|
   Lymph Node                              |                                                                          |   6        |
      Axillary, Hyperplasia, Plasma Cell   |                                                                          |      1  2.0|
      Mediastinal, Hemorrhage              |                                                                          |      3  2.0|
      Renal, Hemorrhage                    |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Lymph Node, Mandibular                  |                                                                          |  50        |
      Hemorrhage                           |                                                                          |      3  2.3|
                                            __________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  |                                                                          |  50        |
      Hemorrhage                           |                                                                          |      3  1.7|
      Hyperplasia, Lymphoid                |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Spleen                                  |                                                                          |  50        |
      Hematopoietic Cell Proliferation     |                                                                          |      2  3.0|
                                            __________________________________________________________________________|____________|
   Thymus                                  |                                                                          |  42        |
      Congestion                           |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Mammary Gland                           |                                                                          |  50        |
      Galactocele                          |                                                                          |     10  1.7|
      Duct, Dilatation                     |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Skin                                    |                                                                          |  50        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Bone                                    |                                                                          |  50        |
      Hyperostosis                         |                                                                          |      5  3.2|
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Brain                                   |                                                                          |  50        |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  37                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8|                                            |      A     |
    3000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 NERVOUS SYSTEM - cont                     |                                                                          |            |
                                           |                                                                          |            |
      Hemorrhage, Focal                    |                                                                          |      2  2.0|
      Hydrocephalus                        |                                                                          |      3  2.7|
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Lung                                    |                                                                          |  50        |
      Congestion                           |                                                                          |      2  2.5|
      Hemorrhage, Focal                    |                                                                          |      2  1.5|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                                                                          |      2  1.5|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Histiocyte                       |                                                                          |      7  1.1|
      Inflammation, Focal                  |                                                                          |     11  1.6|
      Alveolar Epithelium, Hyperplasia,    |                                                                          |            |
           Focal                           |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Nose                                    |                                                                          |  50        |
      Fungus                               |                                                                          |      6  2.5|
      Hemorrhage                           |                                                                          |      2  2.5|
      Inflammation, Acute                  |                                                                          |      2  1.5|
      Inflammation, Chronic Active         |                                                                          |      9  2.6|
      Inflammation, Subacute               |                                                                          |      3  1.7|
      Goblet Cell, Hyperplasia             |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Trachea                                 |                                                                          |  49        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Ear                                     |                                                                          |   1        |
                                            __________________________________________________________________________|____________|
   Eye                                     |                                                                          |   6        |
      Inflammation, Acute                  |                                                                          |      1  3.0|
      Bilateral, Lens, Cataract            |                                                                          |      3  2.0|
      Bilateral, Retina, Atrophy           |                                                                          |      3  3.0|
      Lens, Cataract                       |                                                                          |      1  2.0|
      Retina, Atrophy                      |                                                                          |      1  4.0|
                                            __________________________________________________________________________|____________|
   Zymbal's Gland                          |                                                                          |  47        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Kidney                                  |                                                                          |  50        |
      Congestion                           |                                                                          |      1  2.0|
      Cyst                                 |                                                                          |      1  3.0|
      Cyst, Multiple                       |                                                                          |      1  3.0|
      Mineralization, Focal                |                                                                          |     28  1.4|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  38                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8|                                            |      A     |
    3000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 URINARY SYSTEM - cont                     |                                                                          |            |
                                           |                                                                          |            |
      Nephropathy                          |                                                                          |     41  1.8|
      Pigmentation, Focal                  |                                                                          |     28  1.5|
                                            __________________________________________________________________________|____________|
   Urinary Bladder                         |                                                                          |  50        |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  39                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 7| 7| 7| 5| 6| 6| 5| 7| 7| 5| 6| 5| 7| 7| 6| 6| 6| 7| 2| 6| 7| 5|             
                             DAY ON TEST   | 2| 3| 2| 2| 2| 2| 7| 5| 7| 6| 2| 2| 9| 5| 5| 2| 2| 6| 3| 5| 1| 2| 4| 2| 5|             
                                           | 9| 7| 9| 9| 9| 9| 2| 8| 4| 0| 9| 9| 5| 8| 4| 9| 9| 5| 9| 8| 9| 6| 9| 9| 5|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8|             
    10000PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  A  +  +  A  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     | X  X     X  X  X  X     X     X  X        X  X  X     X  X           X   |             
      Clear Cell Focus                     | X        X                    X                 X                        |             
      Degeneration, Cystic, Focal          |             1                                                            |             
      Eosinophilic Focus                   |                                                                      X   |             
      Fatty Change, Diffuse                |                                                             3            |             
      Fatty Change, Focal                  | 2  2  1     1  1  2  3                 2  2  1     1  2           2  2   |             
      Hepatodiaphragmatic Nodule           |                                                                          |             
      Inflammation, Chronic, Focal         |                   1              1                                       |             
      Mixed Cell Focus                     |                               X                                          |             
      Necrosis, Focal                      |                                              2                 2        1|             
      Bile Duct, Hyperplasia, Focal        |                                                                          |             
      Centrilobular, Necrosis              |                   2                                               3      |             
                                            __________________________________________________________________________|             
   Mesentery                               |    +  +                       +                                      +  +|             
      Fat, Necrosis, Focal                 |    2                          3                                      3  3|             
                                            __________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Accessory Spleen                     |                                                                X         |             
      Acinus, Atrophy, Diffuse             |                                                                          |             
      Acinus, Atrophy, Focal               |          2                       2           1     2     2           2  2|             
      Acinus, Ectopic Liver, Focal         |                                                    2                     |             
      Artery, Inflammation, Focal          |                                                                      2   |             
      Duct, Hyperplasia, Focal             |                                                                1         |             
                                            __________________________________________________________________________|             
   Pharynx                                 |    +                                                              +      |             
                                            __________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                |                                                                          |             
                                            __________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acanthosis, Focal                    |       1                                         2                        |             
      Hyperplasia, Basal Cell, Focal       | 2        1                    1  2     1                    1            |             
      Inflammation, Chronic, Focal         |                               1                                          |             
      Ulcer, Focal                         |    3                                                                     |             
      Ulcer                                |                                                                          |             
                                            __________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 2           2     1  2  2     2           1  2                    2      |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  40                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 7| 7| 7| 5| 6| 6| 5| 7| 7| 5| 6| 5| 7| 7| 6| 6| 6| 7| 2| 6| 7| 5|             
                             DAY ON TEST   | 2| 3| 2| 2| 2| 2| 7| 5| 7| 6| 2| 2| 9| 5| 5| 2| 2| 6| 3| 5| 1| 2| 4| 2| 5|             
                                           | 9| 7| 9| 9| 9| 9| 2| 8| 4| 0| 9| 9| 5| 8| 4| 9| 9| 5| 9| 8| 9| 6| 9| 9| 5|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8|             
    10000PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
                                           |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                         3     2  2  2                                    |             
      Congestion                           |                         2                                                |             
      Hyperplasia, Focal                   | 2                                            2                       1   |             
      Hypertrophy, Focal                   | 1                                2                                   1   |             
      Necrosis, Focal                      |                      3                                                   |             
      Vacuolization Cytoplasmic, Focal     | 2  1           1  2  3  2              3     1     2        2     1  2   |             
                                            __________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          | 2                                      3                                 |             
      Bilateral, Hyperplasia               |                                                                         2|             
                                            __________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |             3                                                            |             
                                            __________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  M  +  +  +  +|             
      Cyst                                 |                                                                          |             
                                            __________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Pars Distalis, Angiectasis           |                                                                          |             
      Pars Distalis, Cyst                  |       2           X                 X           2     3  2               |             
      Pars Distalis, Hyperplasia, Focal    | 2  3              2           2     3           2  2                     |             
                                            __________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      C-Cell, Hyperplasia, Focal           |       2  2     3  2     1     1     2     1                          1   |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Clitoral Gland                          | +  +  +  +  +  +  +  M  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |             3  3                                                         |             
      Inflammation, Chronic                |                3                                                         |             
      Inflammation, Suppurative            |                                                                          |             
      Duct, Dilatation                     |       3                          1                 2        2            |             
                                            __________________________________________________________________________|             
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
      Bilateral, Cyst                      |                   3                                                      |             
                                            __________________________________________________________________________|             
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 | 2     2     2                                                            |             
      Cyst, Multiple                       |                                     2                                    |             
                                            __________________________________________________________________________|             
   Vagina                                  |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |          3           2                                         3        2|             
                                            __________________________________________________________________________|             
   Lymph Node                              |       +                       +  +     +                    +            |             
      Axillary, Hyperplasia, Lymphoid      |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  41                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 7| 7| 7| 5| 6| 6| 5| 7| 7| 5| 6| 5| 7| 7| 6| 6| 6| 7| 2| 6| 7| 5|             
                             DAY ON TEST   | 2| 3| 2| 2| 2| 2| 7| 5| 7| 6| 2| 2| 9| 5| 5| 2| 2| 6| 3| 5| 1| 2| 4| 2| 5|             
                                           | 9| 7| 9| 9| 9| 9| 2| 8| 4| 0| 9| 9| 5| 8| 4| 9| 9| 5| 9| 8| 9| 6| 9| 9| 5|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8|             
    10000PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
                                           |                                                                          |             
      Mediastinal, Hemorrhage              |                               2                                          |             
      Pancreatic, Infiltration Cellular,   |                                                                          |             
           Histiocyte                      |                                  2                                       |             
      Renal, Hemorrhage                    |                                                                          |             
                                            __________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                2                                                         |             
      Hyperplasia, Lymphoid                |                                                                          |             
      Hyperplasia, Plasma Cell             |                   2                                   2              2   |             
                                            __________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                                          |             
                                            __________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
      Hematopoietic Cell Proliferation     |                   3                                                      |             
                                            __________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                       3                  |             
      Necrosis                             |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Galactocele                          |             2  3  3  4  4     2  2           3                       2  3|             
      Hyperplasia, Focal                   |                   3           2                 2                        |             
      Inflammation, Acute, Focal           |                                                       3                  |             
      Inflammation, Chronic, Focal         |    3                                                                     |             
                                            __________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst Epithelial Inclusion            |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperostosis                         |                                                             3            |             
                                            __________________________________________________________________________|             
   Skeletal Muscle                         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hydrocephalus                        |                      2                       2                       2   |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                     2                 3           3      |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Histiocyte                       |                1                 1                       1              1|             
      Inflammation, Focal                  |                      2           1  2     3                              |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                         2                                                |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  42                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 7| 7| 7| 5| 6| 6| 5| 7| 7| 5| 6| 5| 7| 7| 6| 6| 6| 7| 2| 6| 7| 5|             
                             DAY ON TEST   | 2| 3| 2| 2| 2| 2| 7| 5| 7| 6| 2| 2| 9| 5| 5| 2| 2| 6| 3| 5| 1| 2| 4| 2| 5|             
                                           | 9| 7| 9| 9| 9| 9| 2| 8| 4| 0| 9| 9| 5| 8| 4| 9| 9| 5| 9| 8| 9| 6| 9| 9| 5|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8|             
    10000PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
                                           |                                                                          |             
      Arteriole, Mineralization, Focal     |                                                                          |             
                                            __________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |                                                                          |             
      Fungus                               |                   3     3                          3  3              2   |             
      Inflammation, Acute                  |                                                       3           2  3   |             
      Inflammation, Chronic Active         |                   3     3     2                                          |             
      Inflammation, Subacute               |                                                    3                     |             
                                            __________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Zymbal's Gland                          | M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Mineralization, Focal                | 2  1  1        3  2        2  1  1  2     2     2     2  1  1  2        2|             
      Nephropathy                          | 3  1  2  1  2  3  1  2  1     2  3  1     1  4  2        2        4  4  3|             
      Pigmentation, Focal                  | 2     2  2  2     2  3  2  2  2  2  2  2  2  3  2  3  2     2     2  3  2|             
                                            __________________________________________________________________________|             
   Urethra                                 |                                                                          |             
      Cyst                                 |                                                                          |             
                                            __________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +|             
      Hemorrhage                           |                                           4                              |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  43                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 2| 7| 7| 7| 6| 7| 6| 4| 7| 7| 7| 7| 5| 6| 5| 7| 1| 7| 0| 7| 7| 7|             
                             DAY ON TEST   | 2| 2| 2| 9| 2| 2| 2| 1| 2| 6| 8| 2| 2| 2| 2| 0| 6| 0| 2| 7| 0| 8| 2| 2| 2|             
                                           | 9| 9| 9| 6| 9| 9| 9| 8| 9| 0| 4| 9| 9| 9| 9| 2| 3| 2| 9| 6| 2| 5| 9| 9| 9|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9|             
    10000PPM                               | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|             
                                            __________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     |    X  X     X  X  X     X        X  X  X  X        X  X           X  X  X|             
      Clear Cell Focus                     |    X  X     X  X                 X     X  X                             X|             
      Degeneration, Cystic, Focal          |                                                                          |             
      Eosinophilic Focus                   |    X                    X                                                |             
      Fatty Change, Diffuse                |                                                                          |             
      Fatty Change, Focal                  |             1        2        2                    2                     |             
      Hepatodiaphragmatic Nodule           |                                                       X                  |             
      Inflammation, Chronic, Focal         |    2        1           1                          2        1     1  1  1|             
      Mixed Cell Focus                     |    X                                      X                             X|             
      Necrosis, Focal                      |                                                                          |             
      Bile Duct, Hyperplasia, Focal        |                2                       2                                 |             
      Centrilobular, Necrosis              |                                                                          |             
                                            __________________________________________________________________________|             
   Mesentery                               |                                                       +                  |             
      Fat, Necrosis, Focal                 |                                                       2                  |             
                                            __________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|             
      Accessory Spleen                     |                                                                          |             
      Acinus, Atrophy, Diffuse             |                                                                         4|             
      Acinus, Atrophy, Focal               |    3                             2  2           3     2           2      |             
      Acinus, Ectopic Liver, Focal         |                                                                          |             
      Artery, Inflammation, Focal          |                                                                          |             
      Duct, Hyperplasia, Focal             |                                                                          |             
                                            __________________________________________________________________________|             
   Pharynx                                 |                                                                          |             
                                            __________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic                |                               3                                          |             
                                            __________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|             
      Acanthosis, Focal                    |                                                                         2|             
      Hyperplasia, Basal Cell, Focal       |    2              1              2                    1                 1|             
      Inflammation, Chronic, Focal         |                                                                          |             
      Ulcer, Focal                         |                                                    3                     |             
      Ulcer                                |                                                          2               |             
                                            __________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       |    1           2        2        1                                   2   |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  44                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 2| 7| 7| 7| 6| 7| 6| 4| 7| 7| 7| 7| 5| 6| 5| 7| 1| 7| 0| 7| 7| 7|             
                             DAY ON TEST   | 2| 2| 2| 9| 2| 2| 2| 1| 2| 6| 8| 2| 2| 2| 2| 0| 6| 0| 2| 7| 0| 8| 2| 2| 2|             
                                           | 9| 9| 9| 6| 9| 9| 9| 8| 9| 0| 4| 9| 9| 9| 9| 2| 3| 2| 9| 6| 2| 5| 9| 9| 9|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9|             
    10000PPM                               | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM - cont                   |                                                                          |             
                                           |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|             
      Angiectasis, Focal                   |       3                             1                             2      |             
      Congestion                           |       2                                                                  |             
      Hyperplasia, Focal                   |                                     2  1                                2|             
      Hypertrophy, Focal                   |                                        1                                 |             
      Necrosis, Focal                      |                               3                                          |             
      Vacuolization Cytoplasmic, Focal     | 2                 2              2           1        2                 2|             
                                            __________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|             
      Hyperplasia                          |                                              1                           |             
      Bilateral, Hyperplasia               |                                                                          |             
                                            __________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|             
      Hyperplasia, Focal                   |                                                                          |             
                                            __________________________________________________________________________|             
   Parathyroid Gland                       | M  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  M|             
      Cyst                                 |                                                             2            |             
                                            __________________________________________________________________________|             
   Pituitary Gland                         | M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  A  +  +  +|             
      Pars Distalis, Angiectasis           |                2                                                         |             
      Pars Distalis, Cyst                  |    3                                   3                    3        2  2|             
      Pars Distalis, Hyperplasia, Focal    |       2     2  2     3  2  2           2                                 |             
                                            __________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +|             
      C-Cell, Hyperplasia, Focal           |                         2           1                                    |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                   3                                                      |             
      Inflammation, Chronic                |                                                                          |             
      Inflammation, Suppurative            |                                  2                    2                  |             
      Duct, Dilatation                     | 2              2                       2     2                          2|             
                                            __________________________________________________________________________|             
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |             3     3                    2           2                     |             
      Bilateral, Cyst                      |                                                                          |             
                                            __________________________________________________________________________|             
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |             2                    1  1                                    |             
      Cyst, Multiple                       |                                                                   3      |             
                                            __________________________________________________________________________|             
   Vagina                                  |                                     +                                +  +|             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|             
      Atrophy                              |    2                             2           3              1            |             
                                            __________________________________________________________________________|             
   Lymph Node                              | +  +        +  +     +     +                 +  +  +                     |             
      Axillary, Hyperplasia, Lymphoid      |                                              2                           |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  45                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 2| 7| 7| 7| 6| 7| 6| 4| 7| 7| 7| 7| 5| 6| 5| 7| 1| 7| 0| 7| 7| 7|             
                             DAY ON TEST   | 2| 2| 2| 9| 2| 2| 2| 1| 2| 6| 8| 2| 2| 2| 2| 0| 6| 0| 2| 7| 0| 8| 2| 2| 2|             
                                           | 9| 9| 9| 6| 9| 9| 9| 8| 9| 0| 4| 9| 9| 9| 9| 2| 3| 2| 9| 6| 2| 5| 9| 9| 9|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9|             
    10000PPM                               | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |             
                                           |                                                                          |             
      Mediastinal, Hemorrhage              |    1        2  1                                                         |             
      Pancreatic, Infiltration Cellular,   |                                                                          |             
           Histiocyte                      |                                                                          |             
      Renal, Hemorrhage                    |                2                                   3                     |             
                                            __________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                        2                                 |             
      Hyperplasia, Lymphoid                |                                                                         3|             
      Hyperplasia, Plasma Cell             |                                                                          |             
                                            __________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                                         3|             
                                            __________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +|             
      Hematopoietic Cell Proliferation     |                                                                          |             
                                            __________________________________________________________________________|             
   Thymus                                  | +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  A  +  +  +|             
      Hemorrhage                           |                2                                                         |             
      Necrosis                             |          3                                                               |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Galactocele                          |    2              2     2     X  2     1                                2|             
      Hyperplasia, Focal                   |                                                       2                  |             
      Inflammation, Acute, Focal           |                                                                          |             
      Inflammation, Chronic, Focal         |                                                                          |             
                                            __________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst Epithelial Inclusion            |             X                                                            |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperostosis                         |                                                                          |             
                                            __________________________________________________________________________|             
   Skeletal Muscle                         |                                                             +            |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hydrocephalus                        |                                              3     2                     |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                                              2                           |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                               2                                1         |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Histiocyte                       |       1     2  1        1        1        1  1                           |             
      Inflammation, Focal                  |             1  1                 1           2                           |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  46                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 2| 7| 7| 7| 6| 7| 6| 4| 7| 7| 7| 7| 5| 6| 5| 7| 1| 7| 0| 7| 7| 7|             
                             DAY ON TEST   | 2| 2| 2| 9| 2| 2| 2| 1| 2| 6| 8| 2| 2| 2| 2| 0| 6| 0| 2| 7| 0| 8| 2| 2| 2|             
                                           | 9| 9| 9| 6| 9| 9| 9| 8| 9| 0| 4| 9| 9| 9| 9| 2| 3| 2| 9| 6| 2| 5| 9| 9| 9|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9|             
    10000PPM                               | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM - cont                 |                                                                          |             
                                           |                                                                          |             
      Arteriole, Mineralization, Focal     |                                                          1               |             
                                            __________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |                      2                                                   |             
      Fungus                               |    2                                                     2              3|             
      Inflammation, Acute                  |                                                                          |             
      Inflammation, Chronic Active         |    3                       2                             3        2  2  3|             
      Inflammation, Subacute               |                   2  2                                      2            |             
                                            __________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Zymbal's Gland                          | +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Mineralization, Focal                | 1        2  2  2  2  3  1  1  2  1  2  1  2  2     1     2     2     2   |             
      Nephropathy                          | 1  2  1     1  2  3     2  2  1  3  1  1  3  2  2  3  2     2     1  2  3|             
      Pigmentation, Focal                  | 3  2  2     1  1  2  2     2  1  3  1  1  2  3  3  2  2     1     2  2  1|             
                                            __________________________________________________________________________|             
   Urethra                                 |                                  +                                       |             
      Cyst                                 |                                  4                                       |             
                                            __________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  47                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9|                                            |      A     |
    10000PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Esophagus                               |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Colon                  |                                                                          |  48        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Rectum                 |                                                                          |  48        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Cecum                  |                                                                          |  48        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Duodenum               |                                                                          |  45        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Jejunum                |                                                                          |  47        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Ileum                  |                                                                          |  46        |
                                            __________________________________________________________________________|____________|
   Liver                                   |                                                                          |  50        |
      Basophilic Focus                     |                                                                          |     30     |
      Clear Cell Focus                     |                                                                          |     12     |
      Degeneration, Cystic, Focal          |                                                                          |      1  1.0|
      Eosinophilic Focus                   |                                                                          |      3     |
      Fatty Change, Diffuse                |                                                                          |      1  3.0|
      Fatty Change, Focal                  |                                                                          |     18  1.7|
      Hepatodiaphragmatic Nodule           |                                                                          |      1     |
      Inflammation, Chronic, Focal         |                                                                          |     10  1.2|
      Mixed Cell Focus                     |                                                                          |      4     |
      Necrosis, Focal                      |                                                                          |      3  1.7|
      Bile Duct, Hyperplasia, Focal        |                                                                          |      2  2.0|
      Centrilobular, Necrosis              |                                                                          |      2  2.5|
                                            __________________________________________________________________________|____________|
   Mesentery                               |                                                                          |   6        |
      Fat, Necrosis, Focal                 |                                                                          |      5  2.6|
                                            __________________________________________________________________________|____________|
   Pancreas                                |                                                                          |  48        |
      Accessory Spleen                     |                                                                          |      1     |
      Acinus, Atrophy, Diffuse             |                                                                          |      1  4.0|
      Acinus, Atrophy, Focal               |                                                                          |     13  2.1|
      Acinus, Ectopic Liver, Focal         |                                                                          |      1  2.0|
      Artery, Inflammation, Focal          |                                                                          |      1  2.0|
      Duct, Hyperplasia, Focal             |                                                                          |      1  1.0|
                                            __________________________________________________________________________|____________|
   Pharynx                                 |                                                                          |   2        |
                                            __________________________________________________________________________|____________|
   Salivary Glands                         |                                                                          |  50        |
      Inflammation, Chronic                |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Stomach, Forestomach                    |                                                                          |  49        |
      Acanthosis, Focal                    |                                                                          |      3  1.7|
      Hyperplasia, Basal Cell, Focal       |                                                                          |     11  1.4|
      Inflammation, Chronic, Focal         |                                                                          |      1  1.0|
      Ulcer, Focal                         |                                                                          |      2  3.0|
      Ulcer                                |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Stomach, Glandular                      |                                                                          |  48        |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  48                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9|                                            |      A     |
    10000PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
                                           |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Heart                                   |                                                                          |  50        |
      Cardiomyopathy                       |                                                                          |     15  1.7|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Adrenal Cortex                          |                                                                          |  49        |
      Angiectasis, Focal                   |                                                                          |      7  2.1|
      Congestion                           |                                                                          |      2  2.0|
      Hyperplasia, Focal                   |                                                                          |      6  1.7|
      Hypertrophy, Focal                   |                                                                          |      4  1.3|
      Necrosis, Focal                      |                                                                          |      2  3.0|
      Vacuolization Cytoplasmic, Focal     |                                                                          |     18  1.8|
                                            __________________________________________________________________________|____________|
   Adrenal Medulla                         |                                                                          |  49        |
      Hyperplasia                          |                                                                          |      3  2.0|
      Bilateral, Hyperplasia               |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Islets, Pancreatic                      |                                                                          |  48        |
      Hyperplasia, Focal                   |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Parathyroid Gland                       |                                                                          |  44        |
      Cyst                                 |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Pituitary Gland                         |                                                                          |  47        |
      Pars Distalis, Angiectasis           |                                                                          |      1  2.0|
      Pars Distalis, Cyst                  |                                                                          |     11  2.4|
      Pars Distalis, Hyperplasia, Focal    |                                                                          |     14  2.2|
                                            __________________________________________________________________________|____________|
   Thyroid Gland                           |                                                                          |  49        |
      C-Cell, Hyperplasia, Focal           |                                                                          |     11  1.6|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Clitoral Gland                          |                                                                          |  47        |
      Hyperplasia                          |                                                                          |      3  3.0|
      Inflammation, Chronic                |                                                                          |      1  3.0|
      Inflammation, Suppurative            |                                                                          |      2  2.0|
      Duct, Dilatation                     |                                                                          |      9  2.0|
                                            __________________________________________________________________________|____________|
   Ovary                                   |                                                                          |  50        |
      Cyst                                 |                                                                          |      4  2.5|
      Bilateral, Cyst                      |                                                                          |      1  3.0|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  49                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9|                                            |      A     |
    10000PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 GENITAL SYSTEM - cont                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Uterus                                  |                                                                          |  50        |
      Cyst                                 |                                                                          |      6  1.7|
      Cyst, Multiple                       |                                                                          |      2  2.5|
                                            __________________________________________________________________________|____________|
   Vagina                                  |                                                                          |   3        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Bone Marrow                             |                                                                          |  49        |
      Atrophy                              |                                                                          |      8  2.3|
                                            __________________________________________________________________________|____________|
   Lymph Node                              |                                                                          |  14        |
      Axillary, Hyperplasia, Lymphoid      |                                                                          |      1  2.0|
      Mediastinal, Hemorrhage              |                                                                          |      4  1.5|
      Pancreatic, Infiltration Cellular,   |                                                                          |            |
           Histiocyte                      |                                                                          |      1  2.0|
      Renal, Hemorrhage                    |                                                                          |      2  2.5|
                                            __________________________________________________________________________|____________|
   Lymph Node, Mandibular                  |                                                                          |  50        |
      Hemorrhage                           |                                                                          |      2  2.0|
      Hyperplasia, Lymphoid                |                                                                          |      1  3.0|
      Hyperplasia, Plasma Cell             |                                                                          |      3  2.0|
                                            __________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  |                                                                          |  50        |
      Hemorrhage                           |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Spleen                                  |                                                                          |  48        |
      Hematopoietic Cell Proliferation     |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Thymus                                  |                                                                          |  45        |
      Hemorrhage                           |                                                                          |      2  2.5|
      Necrosis                             |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Mammary Gland                           |                                                                          |  50        |
      Galactocele                          |                                                                          |     17  2.4|
      Hyperplasia, Focal                   |                                                                          |      4  2.3|
      Inflammation, Acute, Focal           |                                                                          |      1  3.0|
      Inflammation, Chronic, Focal         |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Skin                                    |                                                                          |  50        |
      Cyst Epithelial Inclusion            |                                                                          |      1     |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Bone                                    |                                                                          |  50        |
      Hyperostosis                         |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Skeletal Muscle                         |                                                                          |   1        |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  50                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS FEMALE                 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 9| 9| 9| 9| 9| 9| 9| 9| 9| 9|                                            |      A     |
    10000PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 MUSCULOSKELETAL SYSTEM - cont             |                                                                          |            |
                                           |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Brain                                   |                                                                          |  50        |
      Hydrocephalus                        |                                                                          |      5  2.2|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Lung                                    |                                                                          |  50        |
      Congestion                           |                                                                          |      5  2.2|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Histiocyte                       |                                                                          |     11  1.1|
      Inflammation, Focal                  |                                                                          |      8  1.6|
      Alveolar Epithelium, Hyperplasia,    |                                                                          |            |
           Focal                           |                                                                          |      1  2.0|
      Arteriole, Mineralization, Focal     |                                                                          |      1  1.0|
                                            __________________________________________________________________________|____________|
   Nose                                    |                                                                          |  50        |
      Foreign Body                         |                                                                          |      1  2.0|
      Fungus                               |                                                                          |      8  2.6|
      Inflammation, Acute                  |                                                                          |      3  2.7|
      Inflammation, Chronic Active         |                                                                          |      9  2.6|
      Inflammation, Subacute               |                                                                          |      4  2.3|
                                            __________________________________________________________________________|____________|
   Trachea                                 |                                                                          |  50        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Zymbal's Gland                          |                                                                          |  46        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Kidney                                  |                                                                          |  50        |
      Mineralization, Focal                |                                                                          |     34  1.7|
      Nephropathy                          |                                                                          |     40  2.1|
      Pigmentation, Focal                  |                                                                          |     42  2.0|
                                            __________________________________________________________________________|____________|
   Urethra                                 |                                                                          |   1        |
      Cyst                                 |                                                                          |      1  4.0|
                                            __________________________________________________________________________|____________|
   Urinary Bladder                         |                                                                          |  49        |
      Hemorrhage                           |                                                                          |      1  4.0|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  51                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 2| 7| 7| 7| 7| 4| 7| 7| 7| 7| 6| 7| 7| 6| 7| 5| 5| 6| 7| 7| 5| 7| 5| 6| 7|             
                             DAY ON TEST   | 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 8| 3| 2| 3| 3| 4| 3| 0| 7| 3|             
                                           | 3| 3| 3| 3| 3| 8| 3| 3| 3| 3| 3| 3| 3| 0| 3| 1| 9| 9| 3| 3| 8| 4| 6| 3| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    0 PPM                                  | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Rectum                 | A  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Cecum                  | A  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Jejunum                | A  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Ileum                  | A  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     |    X  X           X  X  X  X  X     X  X  X           X  X     X     X  X|             
      Clear Cell Focus                     |    X  X              X  X                                               X|             
      Congestion                           |                                                                      2   |             
      Degeneration, Cystic, Focal          |    2     2              1        1  2  1     2  1     2  1     1         |             
      Developmental Malformation           |                                                                   X      |             
      Eosinophilic Focus                   |                   X  X  X                                                |             
      Fatty Change, Diffuse                |                                                                          |             
      Fatty Change, Focal                  |             1           1     1                 1           3  2     2   |             
      Hematopoietic Cell Proliferation     |                                                                          |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                                     2                                    |             
      Inflammation, Chronic, Focal         |                2        2  1        1                                    |             
      Mixed Cell Focus                     |    X                    X                                                |             
      Necrosis, Focal                      | 1                                                                 1      |             
      Pigmentation, Focal                  |                                                                          |             
      Bile Duct, Cyst                      |                                                          3               |             
      Bile Duct, Hyperplasia, Focal        |                                           3                              |             
      Centrilobular, Necrosis              |                                                                          |             
                                            __________________________________________________________________________|             
   Mesentery                               |                         +                                                |             
      Fat, Necrosis, Focal                 |                         2                                                |             
                                            __________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acinus, Atrophy, Diffuse             |                                                                         4|             
      Acinus, Atrophy, Focal               |       2  1  3           3  3     2  2  2  1  3     2     2           3   |             
      Acinus, Vacuolization Cytoplasmic,   |                                                                          |             
           Focal                           |                                                                          |             
      Artery, Inflammation, Focal          |                                                                          |             
                                            __________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy, Focal                       |                                                                          |             
                                            __________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal, Squamous         |    2                                                                     |             
      Inflammation, Chronic, Focal         |    2                                                                     |             
      Ulcer, Focal                         |                                              3              3            |             
                                            __________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  52                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 2| 7| 7| 7| 7| 4| 7| 7| 7| 7| 6| 7| 7| 6| 7| 5| 5| 6| 7| 7| 5| 7| 5| 6| 7|             
                             DAY ON TEST   | 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 8| 3| 2| 3| 3| 4| 3| 0| 7| 3|             
                                           | 3| 3| 3| 3| 3| 8| 3| 3| 3| 3| 3| 3| 3| 0| 3| 1| 9| 9| 3| 3| 8| 4| 6| 3| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    0 PPM                                  | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM - cont              |                                                                          |             
                                           |                                                                          |             
      Cardiomyopathy                       |    2  2  2  2     2  1  2  3     2  3     2  2        2  2  2  2  2  2  2|             
      Mineralization, Focal                |                                                                          |             
      Atrium, Dilatation                   |                                                                      3   |             
      Atrium, Thrombosis                   |                                  2                                       |             
      Valve, Degeneration                  |                                              3                           |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                                          |             
      Hyperplasia, Focal                   |             1                       1                                    |             
      Vacuolization Cytoplasmic, Focal     |             2           1                          2        3  2     2   |             
                                            __________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |          2        1     2           1  2                       2         |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                                     1                                    |             
      Bilateral, Hyperplasia               |                                  2                    2  2               |             
                                            __________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |       2                                                                  |             
                                            __________________________________________________________________________|             
   Parathyroid Gland                       | M  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  M  +|             
      Hyperplasia                          |                                                                          |             
      Bilateral, Hyperplasia               |                                                                          |             
                                            __________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                                                                          |             
      Pars Distalis, Cyst                  |    2                                                                     |             
      Pars Distalis, Hyperplasia, Focal    |    2                 2     2              2  3        2                  |             
      Pars Distalis, Hypertrophy, Focal    |                                                                          |             
                                            __________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      C-Cell, Hyperplasia, Focal           |    1                                2        1                       2   |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration, Focal                  |    2  2  1  2     2  1  2  2  2  2  1     2              1  3  2        2|             
                                            __________________________________________________________________________|             
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |       2                                2                                 |             
      Inflammation, Suppurative            |    2              2                       3                              |             
      Duct, Dilatation                     |                               2  3                 2                     |             
                                            __________________________________________________________________________|             
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |          2           2           2                                       |             
      Degeneration, Focal                  |                                                                          |             
      Hyperplasia, Focal                   |             1     1  1                                                   |             
      Inflammation, Chronic, Focal         |                                                          3               |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  53                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 2| 7| 7| 7| 7| 4| 7| 7| 7| 7| 6| 7| 7| 6| 7| 5| 5| 6| 7| 7| 5| 7| 5| 6| 7|             
                             DAY ON TEST   | 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 8| 3| 2| 3| 3| 4| 3| 0| 7| 3|             
                                           | 3| 3| 3| 3| 3| 8| 3| 3| 3| 3| 3| 3| 3| 0| 3| 1| 9| 9| 3| 3| 8| 4| 6| 3| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    0 PPM                                  | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM - cont                     |                                                                          |             
                                           |                                                                          |             
      Inflammation, Focal, Suppurative     |                   1     2                                      2         |             
      Inflammation, Suppurative            |                                                                          |             
      Vacuolization Cytoplasmic, Focal     |                                                                          |             
                                            __________________________________________________________________________|             
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |          4              3     3           4                              |             
      Cyst, Multiple                       |                            3                                             |             
      Interstitial Cell, Hyperplasia, Focal|                2        1     2                                          |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                                                          |             
                                            __________________________________________________________________________|             
   Lymph Node                              |             +           +  +     +  +  +  +  +     +        +     +  +   |             
      Bronchial, Hemorrhage                |                                                                   3      |             
      Mediastinal, Hemorrhage              |                         2  3        1     2                          2   |             
      Mediastinal, Hyperplasia, Plasma Cell|                                                    3                 3   |             
      Renal, Hemorrhage                    |                                                                          |             
                                            __________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +|             
      Cyst                                 |                      2     2                                             |             
      Hemorrhage                           | 1                                               2                        |             
      Hyperplasia, Plasma Cell             |                      2  2                          3           2     3   |             
                                            __________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                                          |             
      Hyperplasia, Lymphoid                |                                                 2                        |             
      Infiltration Cellular, Histiocyte    |                               2                                          |             
                                            __________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                     2                                    |             
      Fibrosis, Focal                      |                                                                          |             
      Hematopoietic Cell Proliferation     |                                              2                           |             
      Pigmentation, Hemosiderin            |                                                                2         |             
                                            __________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           | 2                                                                        |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +|             
      Galactocele                          |                         2                                         3  2   |             
      Hyperplasia, Focal                   |                                                                          |             
                                            __________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Subcutaneous Tissue, Inflammation,   |                                                                          |             
           Chronic Active, Focal           |                                     2                                    |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperostosis                         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  54                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 2| 7| 7| 7| 7| 4| 7| 7| 7| 7| 6| 7| 7| 6| 7| 5| 5| 6| 7| 7| 5| 7| 5| 6| 7|             
                             DAY ON TEST   | 7| 3| 3| 3| 3| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 8| 3| 2| 3| 3| 4| 3| 0| 7| 3|             
                                           | 3| 3| 3| 3| 3| 8| 3| 3| 3| 3| 3| 3| 3| 0| 3| 1| 9| 9| 3| 3| 8| 4| 6| 3| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
    0 PPM                                  | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM - cont             |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Skeletal Muscle                         |                         +                    +                           |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage, Focal                    |                                                 2                        |             
      Hydrocephalus                        |                                                                2  2  3   |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                                                                          |             
      Necrosis, Focal                      |                                                 3                        |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           | 2                                               2                    3   |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Histiocyte                       |       1                                            1                    1|             
      Inflammation, Focal                  |    1                    1     1                                   1  2   |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                                                                          |             
                                            __________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                                          |             
      Foreign Body                         |                                                          2           2   |             
      Fungus                               |    2     3           2                             3  2  2     2         |             
      Hemorrhage                           | 2                                                                        |             
      Inflammation, Acute                  |                2     2  2                    1                           |             
      Inflammation, Chronic Active         |    3     3                                         3  3  2     2     2   |             
      Inflammation, Subacute               |       2                                                           2      |             
                                            __________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Eye                                     |       +     +     +           +                                          |             
      Phthisis Bulbi                       |             3                                                            |             
      Cornea, Inflammation                 |                                                                          |             
      Lens, Cataract                       |       3                       2                                          |             
      Lens, Inflammation, Focal, Subacute  |                   2                                                      |             
      Retina, Atrophy                      |       2                                                                  |             
                                            __________________________________________________________________________|             
   Zymbal's Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                                                         3|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Nephropathy                          |    3  2  3  3  2  2  2  3  2  3  2  2  2  2  2  1  2  2  3  2  3  2  4  2|             
      Pigmentation, Focal                  |    1                          2           1     1              1  1     1|             
                                            __________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  55                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 5| 7| 7| 7| 5| 6| 7| 6| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 6| 6| 7| 7| 7|             
                             DAY ON TEST   | 3| 7| 3| 3| 1| 7| 1| 3| 7| 3| 3| 7| 3| 3| 3| 3| 0| 3| 3| 7| 1| 8| 3| 3| 3|             
                                           | 4| 2| 4| 4| 5| 0| 4| 4| 9| 4| 4| 3| 4| 4| 4| 4| 7| 4| 4| 2| 8| 2| 4| 4| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1|             
    0 PPM                                  | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  A  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  A  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  A  +  +  +|             
                                            __________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Basophilic Focus                     | X     X              X     X  X  X  X  X  X  X     X  X           X  X  X|             
      Clear Cell Focus                     | X     X                             X  X  X  X                    X  X   |             
      Congestion                           |    2                                                        2            |             
      Degeneration, Cystic, Focal          |                            2           1           1              2      |             
      Developmental Malformation           |                                  X                                       |             
      Eosinophilic Focus                   | X     X  X           X           X  X                                    |             
      Fatty Change, Diffuse                |             3                                                            |             
      Fatty Change, Focal                  |                                     1  1                       1     1   |             
      Hematopoietic Cell Proliferation     |       2                                                                  |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                                                                          |             
      Inflammation, Chronic, Focal         |                                                       1  1               |             
      Mixed Cell Focus                     |       X                                                                 X|             
      Necrosis, Focal                      |                                                                          |             
      Pigmentation, Focal                  |       2                                                                  |             
      Bile Duct, Cyst                      |                                                                          |             
      Bile Duct, Hyperplasia, Focal        |                      3        3  2                 2              2      |             
      Centrilobular, Necrosis              |                                                                2         |             
                                            __________________________________________________________________________|             
   Mesentery                               |       +           +                 +                 +                  |             
      Fat, Necrosis, Focal                 |       2                             3                 3                  |             
                                            __________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acinus, Atrophy, Diffuse             |                                                                          |             
      Acinus, Atrophy, Focal               | 3        3        1        2     3  2  2  1     2  2  2  2     2  3     3|             
      Acinus, Vacuolization Cytoplasmic,   |                                                                          |             
           Focal                           |                                        1                                 |             
      Artery, Inflammation, Focal          |                               2                                          |             
                                            __________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy, Focal                       |                                                                      1   |             
                                            __________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal, Squamous         |                                                                          |             
      Inflammation, Chronic, Focal         |                                                                          |             
      Ulcer, Focal                         |             3           2                                                |             
                                            __________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  56                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 5| 7| 7| 7| 5| 6| 7| 6| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 6| 6| 7| 7| 7|             
                             DAY ON TEST   | 3| 7| 3| 3| 1| 7| 1| 3| 7| 3| 3| 7| 3| 3| 3| 3| 0| 3| 3| 7| 1| 8| 3| 3| 3|             
                                           | 4| 2| 4| 4| 5| 0| 4| 4| 9| 4| 4| 3| 4| 4| 4| 4| 7| 4| 4| 2| 8| 2| 4| 4| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1|             
    0 PPM                                  | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM - cont              |                                                                          |             
                                           |                                                                          |             
      Cardiomyopathy                       | 2     3  3     2  2  2     2     2  2  2     2     2  2     3  2  2  2  2|             
      Mineralization, Focal                |                                                             2            |             
      Atrium, Dilatation                   |                                                                3         |             
      Atrium, Thrombosis                   |             X              3                 4                    3     X|             
      Valve, Degeneration                  |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Adrenal Cortex                          | +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                                4         |             
      Hyperplasia, Focal                   |       2  2           1        3                                          |             
      Vacuolization Cytoplasmic, Focal     |             2                                      2                     |             
                                            __________________________________________________________________________|             
   Adrenal Medulla                         | +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |          2  2        2           2  3        1     2     1     2  2      |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                                                                          |             
      Bilateral, Hyperplasia               |       2                                                                  |             
                                            __________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   | 1           3                          1                                2|             
                                            __________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  M  +  M  +  +  +  +  +  +  +  +  +  +  M  +|             
      Hyperplasia                          |                                                                   2      |             
      Bilateral, Hyperplasia               |                                              2                           |             
                                            __________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                               4                                          |             
      Pars Distalis, Cyst                  |                                     2  2                                 |             
      Pars Distalis, Hyperplasia, Focal    |       3  2  2                                3     1        2            |             
      Pars Distalis, Hypertrophy, Focal    |                                  2                                       |             
                                            __________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      C-Cell, Hyperplasia, Focal           | 1                                                  3     3               |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration, Focal                  | 1     2  2  1     3  2  2  2  2     2     2  2        2           2     2|             
                                            __________________________________________________________________________|             
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |          3                                                               |             
      Inflammation, Suppurative            |             3                                               2            |             
      Duct, Dilatation                     |    2     3                          3        3           3  3     3      |             
                                            __________________________________________________________________________|             
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              | 2     2                    3        3                             2  2  3|             
      Degeneration, Focal                  |             2                                                            |             
      Hyperplasia, Focal                   |                                                                          |             
      Inflammation, Chronic, Focal         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  57                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 5| 7| 7| 7| 5| 6| 7| 6| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 6| 6| 7| 7| 7|             
                             DAY ON TEST   | 3| 7| 3| 3| 1| 7| 1| 3| 7| 3| 3| 7| 3| 3| 3| 3| 0| 3| 3| 7| 1| 8| 3| 3| 3|             
                                           | 4| 2| 4| 4| 5| 0| 4| 4| 9| 4| 4| 3| 4| 4| 4| 4| 7| 4| 4| 2| 8| 2| 4| 4| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1|             
    0 PPM                                  | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM - cont                     |                                                                          |             
                                           |                                                                          |             
      Inflammation, Focal, Suppurative     |                                                                          |             
      Inflammation, Suppurative            | 2  3                                                           2         |             
      Vacuolization Cytoplasmic, Focal     |                                                    2                     |             
                                            __________________________________________________________________________|             
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |                                                                          |             
      Cyst, Multiple                       |                                                                          |             
      Interstitial Cell, Hyperplasia, Focal|                                                          1               |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |    3                                                                     |             
                                            __________________________________________________________________________|             
   Lymph Node                              |             +           +        +                                       |             
      Bronchial, Hemorrhage                |                                                                          |             
      Mediastinal, Hemorrhage              |                                                                          |             
      Mediastinal, Hyperplasia, Plasma Cell|                                                                          |             
      Renal, Hemorrhage                    |                                  2                                       |             
                                            __________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M|             
      Cyst                                 |                                              2                           |             
      Hemorrhage                           |                                  2                                       |             
      Hyperplasia, Plasma Cell             |                                  2                 3     2     2         |             
                                            __________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +     +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                                   3  3   |             
      Hyperplasia, Lymphoid                |                                                                          |             
      Infiltration Cellular, Histiocyte    |                                                                          |             
                                            __________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                                          |             
      Fibrosis, Focal                      |                                              3                    2      |             
      Hematopoietic Cell Proliferation     |    3                                                                     |             
      Pigmentation, Hemosiderin            |                                                                          |             
                                            __________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +|             
      Hemorrhage                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Galactocele                          |                                                    2     4           4   |             
      Hyperplasia, Focal                   | 2                                                                        |             
                                            __________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Subcutaneous Tissue, Inflammation,   |                                                                          |             
           Chronic Active, Focal           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperostosis                         | 2                                                                        |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  58                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 5| 7| 7| 7| 5| 6| 7| 6| 7| 7| 6| 7| 7| 7| 7| 6| 7| 7| 5| 6| 6| 7| 7| 7|             
                             DAY ON TEST   | 3| 7| 3| 3| 1| 7| 1| 3| 7| 3| 3| 7| 3| 3| 3| 3| 0| 3| 3| 7| 1| 8| 3| 3| 3|             
                                           | 4| 2| 4| 4| 5| 0| 4| 4| 9| 4| 4| 3| 4| 4| 4| 4| 7| 4| 4| 2| 8| 2| 4| 4| 4|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1|             
    0 PPM                                  | 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM - cont             |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Skeletal Muscle                         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage, Focal                    |                                                                          |             
      Hydrocephalus                        |                                                          2               |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                                                          2               |             
      Necrosis, Focal                      |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |    2                                                        3  4         |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Histiocyte                       |                                           2              1           2   |             
      Inflammation, Focal                  |       2                                                                 1|             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                                           2                          2   |             
                                            __________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                                3         |             
      Foreign Body                         | 2                                2                                   3   |             
      Fungus                               |                                                                          |             
      Hemorrhage                           |          2                                                               |             
      Inflammation, Acute                  |                                              2  2                    3   |             
      Inflammation, Chronic Active         |             2           3                                                |             
      Inflammation, Subacute               | 2                                2                          2            |             
                                            __________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Eye                                     |                                                    +                     |             
      Phthisis Bulbi                       |                                                                          |             
      Cornea, Inflammation                 |                                                    2                     |             
      Lens, Cataract                       |                                                    3                     |             
      Lens, Inflammation, Focal, Subacute  |                                                                          |             
      Retina, Atrophy                      |                                                                          |             
                                            __________________________________________________________________________|             
   Zymbal's Gland                          | +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +|             
      Hyperplasia                          |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Nephropathy                          | 2  2  3  3  2  2  2  2     4  3  3  2  1  2  4  2  2  2  3  2  3  3  2  3|             
      Pigmentation, Focal                  |             2                    1                    1     2           2|             
                                            __________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  59                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|                                            |      A     |
    0 PPM                                  | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Esophagus                               |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Colon                  |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Rectum                 |                                                                          |  46        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Cecum                  |                                                                          |  46        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Duodenum               |                                                                          |  49        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Jejunum                |                                                                          |  47        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Ileum                  |                                                                          |  46        |
                                            __________________________________________________________________________|____________|
   Liver                                   |                                                                          |  50        |
      Basophilic Focus                     |                                                                          |     30     |
      Clear Cell Focus                     |                                                                          |     13     |
      Congestion                           |                                                                          |      3  2.0|
      Degeneration, Cystic, Focal          |                                                                          |     15  1.5|
      Developmental Malformation           |                                                                          |      2     |
      Eosinophilic Focus                   |                                                                          |      9     |
      Fatty Change, Diffuse                |                                                                          |      1  3.0|
      Fatty Change, Focal                  |                                                                          |     11  1.4|
      Hematopoietic Cell Proliferation     |                                                                          |      1  2.0|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                                                                          |      1  2.0|
      Inflammation, Chronic, Focal         |                                                                          |      6  1.3|
      Mixed Cell Focus                     |                                                                          |      4     |
      Necrosis, Focal                      |                                                                          |      2  1.0|
      Pigmentation, Focal                  |                                                                          |      1  2.0|
      Bile Duct, Cyst                      |                                                                          |      1  3.0|
      Bile Duct, Hyperplasia, Focal        |                                                                          |      6  2.5|
      Centrilobular, Necrosis              |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Mesentery                               |                                                                          |   5        |
      Fat, Necrosis, Focal                 |                                                                          |      4  2.5|
                                            __________________________________________________________________________|____________|
   Pancreas                                |                                                                          |  50        |
      Acinus, Atrophy, Diffuse             |                                                                          |      1  4.0|
      Acinus, Atrophy, Focal               |                                                                          |     28  2.2|
      Acinus, Vacuolization Cytoplasmic,   |                                                                          |            |
           Focal                           |                                                                          |      1  1.0|
      Artery, Inflammation, Focal          |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Salivary Glands                         |                                                                          |  50        |
      Atrophy, Focal                       |                                                                          |      1  1.0|
                                            __________________________________________________________________________|____________|
   Stomach, Forestomach                    |                                                                          |  50        |
      Hyperplasia, Focal, Squamous         |                                                                          |      1  2.0|
      Inflammation, Chronic, Focal         |                                                                          |      1  2.0|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  60                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|                                            |      A     |
    0 PPM                                  | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
                                           |                                                                          |            |
      Ulcer, Focal                         |                                                                          |      4  2.8|
                                            __________________________________________________________________________|____________|
   Stomach, Glandular                      |                                                                          |  50        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Heart                                   |                                                                          |  50        |
      Cardiomyopathy                       |                                                                          |     37  2.1|
      Mineralization, Focal                |                                                                          |      1  2.0|
      Atrium, Dilatation                   |                                                                          |      2  3.0|
      Atrium, Thrombosis                   |                                                                          |      6  3.0|
      Valve, Degeneration                  |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Adrenal Cortex                          |                                                                          |  49        |
      Hemorrhage                           |                                                                          |      1  4.0|
      Hyperplasia, Focal                   |                                                                          |      6  1.7|
      Vacuolization Cytoplasmic, Focal     |                                                                          |      8  2.0|
                                            __________________________________________________________________________|____________|
   Adrenal Medulla                         |                                                                          |  49        |
      Hyperplasia                          |                                                                          |     16  1.8|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                                                                          |      1  1.0|
      Bilateral, Hyperplasia               |                                                                          |      4  2.0|
                                            __________________________________________________________________________|____________|
   Islets, Pancreatic                      |                                                                          |  50        |
      Hyperplasia, Focal                   |                                                                          |      5  1.8|
                                            __________________________________________________________________________|____________|
   Parathyroid Gland                       |                                                                          |  43        |
      Hyperplasia                          |                                                                          |      1  2.0|
      Bilateral, Hyperplasia               |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Pituitary Gland                         |                                                                          |  50        |
      Angiectasis, Focal                   |                                                                          |      1  4.0|
      Pars Distalis, Cyst                  |                                                                          |      3  2.0|
      Pars Distalis, Hyperplasia, Focal    |                                                                          |     12  2.2|
      Pars Distalis, Hypertrophy, Focal    |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Thyroid Gland                           |                                                                          |  50        |
      C-Cell, Hyperplasia, Focal           |                                                                          |      7  1.9|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Epididymis                              |                                                                          |  50        |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  61                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|                                            |      A     |
    0 PPM                                  | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 GENITAL SYSTEM - cont                     |                                                                          |            |
                                           |                                                                          |            |
      Degeneration, Focal                  |                                                                          |     31  1.9|
                                            __________________________________________________________________________|____________|
   Preputial Gland                         |                                                                          |  50        |
      Hyperplasia                          |                                                                          |      3  2.3|
      Inflammation, Suppurative            |                                                                          |      5  2.4|
      Duct, Dilatation                     |                                                                          |     10  2.7|
                                            __________________________________________________________________________|____________|
   Prostate                                |                                                                          |  50        |
      Atrophy                              |                                                                          |     10  2.3|
      Degeneration, Focal                  |                                                                          |      1  2.0|
      Hyperplasia, Focal                   |                                                                          |      3  1.0|
      Inflammation, Chronic, Focal         |                                                                          |      1  3.0|
      Inflammation, Focal, Suppurative     |                                                                          |      3  1.7|
      Inflammation, Suppurative            |                                                                          |      3  2.3|
      Vacuolization Cytoplasmic, Focal     |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Seminal Vesicle                         |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Testes                                  |                                                                          |  50        |
      Atrophy                              |                                                                          |      4  3.5|
      Cyst, Multiple                       |                                                                          |      1  3.0|
      Interstitial Cell, Hyperplasia, Focal|                                                                          |      4  1.5|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Bone Marrow                             |                                                                          |  50        |
      Hyperplasia                          |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Lymph Node                              |                                                                          |  15        |
      Bronchial, Hemorrhage                |                                                                          |      1  3.0|
      Mediastinal, Hemorrhage              |                                                                          |      5  2.0|
      Mediastinal, Hyperplasia, Plasma Cell|                                                                          |      2  3.0|
      Renal, Hemorrhage                    |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Lymph Node, Mandibular                  |                                                                          |  48        |
      Cyst                                 |                                                                          |      3  2.0|
      Hemorrhage                           |                                                                          |      3  1.7|
      Hyperplasia, Plasma Cell             |                                                                          |      9  2.3|
                                            __________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  |                                                                          |  49        |
      Hemorrhage                           |                                                                          |      2  3.0|
      Hyperplasia, Lymphoid                |                                                                          |      1  2.0|
      Infiltration Cellular, Histiocyte    |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Spleen                                  |                                                                          |  50        |
      Congestion                           |                                                                          |      1  2.0|
      Fibrosis, Focal                      |                                                                          |      2  2.5|
      Hematopoietic Cell Proliferation     |                                                                          |      2  2.5|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  62                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|                                            |      A     |
    0 PPM                                  | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
                                           |                                                                          |            |
      Pigmentation, Hemosiderin            |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Thymus                                  |                                                                          |  48        |
      Hemorrhage                           |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Mammary Gland                           |                                                                          |  48        |
      Galactocele                          |                                                                          |      6  2.8|
      Hyperplasia, Focal                   |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Skin                                    |                                                                          |  50        |
      Subcutaneous Tissue, Inflammation,   |                                                                          |            |
           Chronic Active, Focal           |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Bone                                    |                                                                          |  50        |
      Hyperostosis                         |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Skeletal Muscle                         |                                                                          |   2        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Brain                                   |                                                                          |  50        |
      Hemorrhage, Focal                    |                                                                          |      1  2.0|
      Hydrocephalus                        |                                                                          |      4  2.3|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                                                                          |      1  2.0|
      Necrosis, Focal                      |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Lung                                    |                                                                          |  50        |
      Congestion                           |                                                                          |      6  2.7|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Histiocyte                       |                                                                          |      6  1.3|
      Inflammation, Focal                  |                                                                          |      7  1.3|
      Alveolar Epithelium, Hyperplasia,    |                                                                          |            |
           Focal                           |                                                                          |      2  2.0|
                                            __________________________________________________________________________|____________|
   Nose                                    |                                                                          |  50        |
      Congestion                           |                                                                          |      1  3.0|
      Foreign Body                         |                                                                          |      5  2.2|
      Fungus                               |                                                                          |      7  2.3|
      Hemorrhage                           |                                                                          |      2  2.0|
      Inflammation, Acute                  |                                                                          |      7  2.0|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  63                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|                                            |      A     |
    0 PPM                                  | 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 RESPIRATORY SYSTEM - cont                 |                                                                          |            |
                                           |                                                                          |            |
      Inflammation, Chronic Active         |                                                                          |      9  2.6|
      Inflammation, Subacute               |                                                                          |      5  2.0|
                                            __________________________________________________________________________|____________|
   Trachea                                 |                                                                          |  50        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Eye                                     |                                                                          |   5        |
      Phthisis Bulbi                       |                                                                          |      1  3.0|
      Cornea, Inflammation                 |                                                                          |      1  2.0|
      Lens, Cataract                       |                                                                          |      3  2.7|
      Lens, Inflammation, Focal, Subacute  |                                                                          |      1  2.0|
      Retina, Atrophy                      |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Zymbal's Gland                          |                                                                          |  48        |
      Hyperplasia                          |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Kidney                                  |                                                                          |  50        |
      Nephropathy                          |                                                                          |     48  2.4|
      Pigmentation, Focal                  |                                                                          |     12  1.3|
                                            __________________________________________________________________________|____________|
   Urinary Bladder                         |                                                                          |  50        |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  64                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 6| 6| 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 6| 6| 7| 7| 7| 7|             
                             DAY ON TEST   | 2| 0| 3| 3| 3| 4| 3| 3| 3| 2| 3| 3| 1| 3| 5| 8| 3| 3| 3| 7| 5| 3| 3| 3| 3|             
                                           | 1| 1| 7| 0| 0| 1| 0| 0| 7| 1| 0| 0| 3| 0| 1| 5| 0| 0| 0| 3| 1| 3| 3| 3| 3|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    1000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                                                                   2      |             
      Basophilic Focus                     |       X  X        X  X  X        X     X        X  X  X        X  X  X  X|             
      Clear Cell Focus                     |          X                             X        X  X                    X|             
      Congestion                           |    2                                                                     |             
      Degeneration, Cystic, Focal          |                1  2     1     2     2                 1        1        2|             
      Developmental Malformation           |                                                                          |             
      Eosinophilic Focus                   |                      X        X  X     X  X     X     X           X  X  X|             
      Fatty Change, Diffuse                |                                                          3               |             
      Fatty Change, Focal                  |                                  1                          3            |             
      Inflammation, Chronic, Focal         |                                                       1                  |             
      Mixed Cell Focus                     |                                                       X                  |             
      Necrosis, Focal                      |                2                                                         |             
      Bile Duct, Hyperplasia, Focal        |                               2                          3               |             
                                            __________________________________________________________________________|             
   Mesentery                               |                                        +     +  +        +               |             
      Fat, Necrosis, Focal                 |                                        3     3  3        3               |             
                                            __________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Accessory Spleen                     |                                                                          |             
      Acinus, Atrophy, Diffuse             |                                                                4         |             
      Acinus, Atrophy, Focal               |          2  1     2  2     2  2  1  2  2  2     2     3     2     2     1|             
                                            __________________________________________________________________________|             
   Pharynx                                 |          +                                                               |             
      Palate, Inflammation, Chronic Active |          3                                                               |             
                                            __________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acanthosis, Focal                    |                                                                          |             
      Hyperplasia, Basal Cell, Focal       |                   1                                                      |             
      Hyperplasia, Focal, Squamous         |                                                                          |             
      Inflammation, Chronic, Focal         |                                                                          |             
      Inflammation, Chronic Active, Focal  |                                                                          |             
      Ulcer, Focal                         |                                                          3               |             
                                            __________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Erosion, Focal                       |                                                             2            |             
      Inflammation, Acute, Focal           |                                                                          |             
      Ulcer, Focal                         |                                                             2            |             
                                            __________________________________________________________________________|             
   Tooth                                   |          +                                                               |             
      Gingiva, Inflammation, Chronic Active|          2                                                               |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  65                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 6| 6| 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 6| 6| 7| 7| 7| 7|             
                             DAY ON TEST   | 2| 0| 3| 3| 3| 4| 3| 3| 3| 2| 3| 3| 1| 3| 5| 8| 3| 3| 3| 7| 5| 3| 3| 3| 3|             
                                           | 1| 1| 7| 0| 0| 1| 0| 0| 7| 1| 0| 0| 3| 0| 1| 5| 0| 0| 0| 3| 1| 3| 3| 3| 3|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    1000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM - cont              |                                                                          |             
                                           |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 2  2  2  1        2  2  1     1     1  2  2  2  2  1  1     1        2  1|             
      Atrium, Dilatation                   |                                     3                                    |             
      Atrium, Thrombosis                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                                                                          |             
      Congestion                           |                         1                                                |             
      Cyst                                 |                                                                          |             
      Degeneration, Cystic, Focal          |                            2                                             |             
      Hyperplasia, Focal                   |                                                                         2|             
      Vacuolization Cytoplasmic, Focal     |                            2           2                 3        1     1|             
                                            __________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |       2                 1        1     2              3  2               |             
      Bilateral, Hyperplasia               | 2                    2     2                                             |             
                                            __________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |                      2                                      3            |             
                                            __________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                                                                          |             
      Pigmentation, Focal                  |                                                                          |             
      Pars Distalis, Cyst                  |                                                                          |             
      Pars Distalis, Hyperplasia, Focal    |       2     3     1  1        2        2              2     2           2|             
                                            __________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      C-Cell, Hyperplasia, Focal           |                                                    2     2               |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Coagulating Gland                       |                                              +                           |             
                                            __________________________________________________________________________|             
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration, Focal                  |    2  2  2  2  2     2  2  2  2  1  2     2     2  2  2  3  2     2  1  3|             
                                            __________________________________________________________________________|             
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                                       3                  |             
      Inflammation, Suppurative            |                                                                          |             
      Duct, Dilatation                     | 2        3  2                       2  2  2     2        2  2            |             
                                            __________________________________________________________________________|             
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Abscess                              |                                                                          |             
      Atrophy                              |             2              2        2                                    |             
      Degeneration, Focal                  |                                  1                                       |             
      Inflammation, Chronic, Focal         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  66                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 6| 6| 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 6| 6| 7| 7| 7| 7|             
                             DAY ON TEST   | 2| 0| 3| 3| 3| 4| 3| 3| 3| 2| 3| 3| 1| 3| 5| 8| 3| 3| 3| 7| 5| 3| 3| 3| 3|             
                                           | 1| 1| 7| 0| 0| 1| 0| 0| 7| 1| 0| 0| 3| 0| 1| 5| 0| 0| 0| 3| 1| 3| 3| 3| 3|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    1000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM - cont                     |                                                                          |             
                                           |                                                                          |             
      Inflammation, Focal, Suppurative     |                                                                          |             
                                            __________________________________________________________________________|             
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |                                                                          |             
                                            __________________________________________________________________________|             
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |                      3                                                  3|             
      Mineralization, Focal                |                                                                          |             
      Bilateral, Atrophy                   |                                                                          |             
      Interstitial Cell, Hyperplasia, Focal|                                                                2         |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Blood                                   |                                                                          |             
                                            __________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Lymph Node                              |             +           +  +        +        +           +  +            |             
      Bronchial, Infiltration Cellular,    |                                                                          |             
           Histiocyte                      |                                                                          |             
      Mediastinal, Hemorrhage              |                                                                          |             
      Renal, Hemorrhage                    |                                                                          |             
                                            __________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |    2                                                                     |             
      Hemorrhage                           |                                                                          |             
      Hyperplasia, Plasma Cell             | 3  3     3        2           2        2                                 |             
                                            __________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                  3                                       |             
      Hemorrhage                           |                                                                         3|             
                                            __________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Depletion Cellular                   |                                                                      3   |             
      Fibrosis, Focal                      |                                                                          |             
      Hematopoietic Cell Proliferation     |                                                                          |             
                                            __________________________________________________________________________|             
   Thymus                                  | +  +  +  M  +  +  +  +  +  +  +  +  +  M  +  +  +  +  M  +  +  M  +  +  +|             
      Hemorrhage                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Galactocele                          | 3                 2  2                                                   |             
      Hyperplasia, Focal                   |             3                                                  1         |             
      Inflammation, Acute, Focal           | 2                                                                        |             
                                            __________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Skeletal Muscle                         |                                                       +                  |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  67                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 6| 6| 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 6| 6| 7| 7| 7| 7|             
                             DAY ON TEST   | 2| 0| 3| 3| 3| 4| 3| 3| 3| 2| 3| 3| 1| 3| 5| 8| 3| 3| 3| 7| 5| 3| 3| 3| 3|             
                                           | 1| 1| 7| 0| 0| 1| 0| 0| 7| 1| 0| 0| 3| 0| 1| 5| 0| 0| 0| 3| 1| 3| 3| 3| 3|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1|             
    1000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM - cont                     |                                                                          |             
                                           |                                                                          |             
      Hydrocephalus                        |                                                                          |             
      Necrosis, Focal                      |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                                          |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                                                                          |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Histiocyte                       | 1                                                  1                    1|             
      Inflammation, Focal                  |          1                                      2                        |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                                                                          |             
                                            __________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |                2                                                         |             
      Fungus                               |                               2                       3  3           2   |             
      Hemorrhage                           |                                                                          |             
      Inflammation, Acute                  |                2                                                     2   |             
      Inflammation, Chronic Active         |             2              2  2                       3  3               |             
      Inflammation, Subacute               | 2                                                                        |             
      Olfactory Epithelium, Necrosis, Focal| 2                                                                        |             
                                            __________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Eye                                     |    +     +                                                               |             
      Inflammation, Chronic                |    3                                                                     |             
      Phthisis Bulbi                       |                                                                          |             
      Lens, Cataract                       |                                                                          |             
      Retina, Atrophy                      |          3                                                               |             
                                            __________________________________________________________________________|             
   Harderian Gland                         |    +                                                                     |             
                                            __________________________________________________________________________|             
   Zymbal's Gland                          | +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Nephropathy                          | 3  2  2  3  4  2  3  2  1  3  2  2  2  1  1  2  2  2  2  2  2  2  2  3  3|             
      Pigmentation, Focal                  |                         2           3                    2  4            |             
                                            __________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  68                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 7| 6| 7| 3| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 6| 7| 6| 7| 6|             
                             DAY ON TEST   | 1| 1| 1| 2| 1| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 7| 7| 6| 1| 3| 4| 3| 7|             
                                           | 5| 7| 4| 6| 4| 3| 9| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 1| 0| 0| 8| 3| 6| 3| 1|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    1000 PPM                               | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Rectum                 | +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                2                                                         |             
      Basophilic Focus                     |          X     X        X  X  X  X  X  X  X  X  X              X         |             
      Clear Cell Focus                     |                         X     X  X  X  X     X  X              X         |             
      Congestion                           |       3  2                                                  2            |             
      Degeneration, Cystic, Focal          |       1                 2                       1        2              2|             
      Developmental Malformation           |          X                                                               |             
      Eosinophilic Focus                   |                            X                                             |             
      Fatty Change, Diffuse                | 4  3        2                                         3                  |             
      Fatty Change, Focal                  |       1  1           3           1  1  1  1              1  2            |             
      Inflammation, Chronic, Focal         |                                                             1            |             
      Mixed Cell Focus                     |                                                                          |             
      Necrosis, Focal                      |                                     1                          1         |             
      Bile Duct, Hyperplasia, Focal        |                                                                         3|             
                                            __________________________________________________________________________|             
   Mesentery                               |                                        +                                 |             
      Fat, Necrosis, Focal                 |                                        2                                 |             
                                            __________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Accessory Spleen                     |                                                                X         |             
      Acinus, Atrophy, Diffuse             |                                                                          |             
      Acinus, Atrophy, Focal               | 2     2  2  1     1  2  2  2                          1        1        1|             
                                            __________________________________________________________________________|             
   Pharynx                                 |                                                                          |             
      Palate, Inflammation, Chronic Active |                                                                          |             
                                            __________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acanthosis, Focal                    | 3                                                                        |             
      Hyperplasia, Basal Cell, Focal       |                                                                      1   |             
      Hyperplasia, Focal, Squamous         |                                              3                           |             
      Inflammation, Chronic, Focal         |                                                    2                     |             
      Inflammation, Chronic Active, Focal  | 3                                                           2            |             
      Ulcer, Focal                         | 3                                                                       2|             
                                            __________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Erosion, Focal                       |                                                                   2      |             
      Inflammation, Acute, Focal           |                                                       2                  |             
      Ulcer, Focal                         | 2                                                                       2|             
                                            __________________________________________________________________________|             
   Tooth                                   |                                                                          |             
      Gingiva, Inflammation, Chronic Active|                                                                          |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  69                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 7| 6| 7| 3| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 6| 7| 6| 7| 6|             
                             DAY ON TEST   | 1| 1| 1| 2| 1| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 7| 7| 6| 1| 3| 4| 3| 7|             
                                           | 5| 7| 4| 6| 4| 3| 9| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 1| 0| 0| 8| 3| 6| 3| 1|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    1000 PPM                               | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM - cont              |                                                                          |             
                                           |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       |    2  2  2  3  1        1     2  2     1  2  2              2  2         |             
      Atrium, Dilatation                   |          3     3                                                         |             
      Atrium, Thrombosis                   |       X                 2                                                |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                2                                                         |             
      Congestion                           |                                                                          |             
      Cyst                                 |          3                                                               |             
      Degeneration, Cystic, Focal          |                                                             1            |             
      Hyperplasia, Focal                   |       2        1        1                       2                    1   |             
      Vacuolization Cytoplasmic, Focal     | 2                             1     2                 2  3  2     2      |             
                                            __________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |       2        2     2        2        1                       2         |             
      Bilateral, Hyperplasia               |                                                                          |             
                                            __________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |                                              2           1               |             
                                            __________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  M  +  +  +|             
                                            __________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                   2                                                      |             
      Pigmentation, Focal                  |             2                                                            |             
      Pars Distalis, Cyst                  |                                              2                       3   |             
      Pars Distalis, Hyperplasia, Focal    |                         2  1        1  3     1  2              3         |             
                                            __________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      C-Cell, Hyperplasia, Focal           |             3           2                                      2        2|             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Coagulating Gland                       |                                                                          |             
                                            __________________________________________________________________________|             
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration, Focal                  | 2  3  2  2     2           2     2     1  2  2  3  2  2     3  1  3  3  2|             
                                            __________________________________________________________________________|             
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                                                          |             
      Inflammation, Suppurative            |                                           4     3  2                     |             
      Duct, Dilatation                     |    3     3           2                 2     2  3        3           3   |             
                                            __________________________________________________________________________|             
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Abscess                              |                                                          3               |             
      Atrophy                              |                2                             2  2                    2   |             
      Degeneration, Focal                  |                                                                          |             
      Inflammation, Chronic, Focal         |                                  3                                       |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  70                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 7| 6| 7| 3| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 6| 7| 6| 7| 6|             
                             DAY ON TEST   | 1| 1| 1| 2| 1| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 7| 7| 6| 1| 3| 4| 3| 7|             
                                           | 5| 7| 4| 6| 4| 3| 9| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 1| 0| 0| 8| 3| 6| 3| 1|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    1000 PPM                               | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM - cont                     |                                                                          |             
                                           |                                                                          |             
      Inflammation, Focal, Suppurative     |                   2                       2                              |             
                                            __________________________________________________________________________|             
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |                                                       2                  |             
                                            __________________________________________________________________________|             
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |                3                                                         |             
      Mineralization, Focal                |                                                             2            |             
      Bilateral, Atrophy                   | 2                                                           2            |             
      Interstitial Cell, Hyperplasia, Focal|                                                       2                  |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Blood                                   |                                                       +                  |             
                                            __________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Lymph Node                              |    +  +  +  +  +                                   +  +           +     +|             
      Bronchial, Infiltration Cellular,    |                                                                          |             
           Histiocyte                      |                3                                                         |             
      Mediastinal, Hemorrhage              |       3  3                                                               |             
      Renal, Hemorrhage                    |                                                       2                  |             
                                            __________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                     2                                    |             
      Hemorrhage                           |       3  2                                                               |             
      Hyperplasia, Plasma Cell             |                         2        2        2                 2            |             
                                            __________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
      Hemorrhage                           |       3                                                                  |             
                                            __________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Depletion Cellular                   |                                                                          |             
      Fibrosis, Focal                      |                                  2                                       |             
      Hematopoietic Cell Proliferation     |          2                                                               |             
                                            __________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  M  +  M  M  M  M  M  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |          2                                                               |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Galactocele                          | 2              3  2                                         2            |             
      Hyperplasia, Focal                   |                                                                          |             
      Inflammation, Acute, Focal           |                                                                          |             
                                            __________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Skeletal Muscle                         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  71                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 7| 6| 7| 3| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 6| 7| 6| 7| 6|             
                             DAY ON TEST   | 1| 1| 1| 2| 1| 1| 6| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 7| 7| 6| 1| 3| 4| 3| 7|             
                                           | 5| 7| 4| 6| 4| 3| 9| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 1| 0| 0| 8| 3| 6| 3| 1|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    1000 PPM                               | 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM - cont                     |                                                                          |             
                                           |                                                                          |             
      Hydrocephalus                        | 2                 2                                   3     2            |             
      Necrosis, Focal                      |                   3                                                      |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |          2                                                               |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                         1                 1                    1         |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Histiocyte                       |                                        1                                 |             
      Inflammation, Focal                  |                                                                          |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                                        1                                 |             
                                            __________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |                                                                   3      |             
      Fungus                               |    2                 3                                                   |             
      Hemorrhage                           |                                                                3         |             
      Inflammation, Acute                  |                                                                          |             
      Inflammation, Chronic Active         |    3     3           4              3                             3      |             
      Inflammation, Subacute               |                                                                          |             
      Olfactory Epithelium, Necrosis, Focal|                                                                          |             
                                            __________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Eye                                     |                         +                                      +         |             
      Inflammation, Chronic                |                                                                          |             
      Phthisis Bulbi                       |                                                                3         |             
      Lens, Cataract                       |                         4                                                |             
      Retina, Atrophy                      |                         3                                                |             
                                            __________________________________________________________________________|             
   Harderian Gland                         |                                                                          |             
                                            __________________________________________________________________________|             
   Zymbal's Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Nephropathy                          | 3  2  2  3  2  4  3  1  2  3  2  2  2  2  3  2  2  3  3  4  2  2  3  4  2|             
      Pigmentation, Focal                  |    2        3                                      3  3  3        2     2|             
                                            __________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  72                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                                            |      A     |
    1000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Esophagus                               |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Colon                  |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Rectum                 |                                                                          |  49        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Cecum                  |                                                                          |  49        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Duodenum               |                                                                          |  49        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Jejunum                |                                                                          |  49        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Ileum                  |                                                                          |  49        |
                                            __________________________________________________________________________|____________|
   Liver                                   |                                                                          |  50        |
      Angiectasis, Focal                   |                                                                          |      2  2.0|
      Basophilic Focus                     |                                                                          |     26     |
      Clear Cell Focus                     |                                                                          |     13     |
      Congestion                           |                                                                          |      4  2.3|
      Degeneration, Cystic, Focal          |                                                                          |     13  1.5|
      Developmental Malformation           |                                                                          |      1     |
      Eosinophilic Focus                   |                                                                          |     11     |
      Fatty Change, Diffuse                |                                                                          |      5  3.0|
      Fatty Change, Focal                  |                                                                          |     11  1.5|
      Inflammation, Chronic, Focal         |                                                                          |      2  1.0|
      Mixed Cell Focus                     |                                                                          |      1     |
      Necrosis, Focal                      |                                                                          |      3  1.3|
      Bile Duct, Hyperplasia, Focal        |                                                                          |      3  2.7|
                                            __________________________________________________________________________|____________|
   Mesentery                               |                                                                          |   5        |
      Fat, Necrosis, Focal                 |                                                                          |      5  2.8|
                                            __________________________________________________________________________|____________|
   Pancreas                                |                                                                          |  50        |
      Accessory Spleen                     |                                                                          |      1     |
      Acinus, Atrophy, Diffuse             |                                                                          |      1  4.0|
      Acinus, Atrophy, Focal               |                                                                          |     26  1.7|
                                            __________________________________________________________________________|____________|
   Pharynx                                 |                                                                          |   1        |
      Palate, Inflammation, Chronic Active |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Salivary Glands                         |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Stomach, Forestomach                    |                                                                          |  50        |
      Acanthosis, Focal                    |                                                                          |      1  3.0|
      Hyperplasia, Basal Cell, Focal       |                                                                          |      2  1.0|
      Hyperplasia, Focal, Squamous         |                                                                          |      1  3.0|
      Inflammation, Chronic, Focal         |                                                                          |      1  2.0|
      Inflammation, Chronic Active, Focal  |                                                                          |      2  2.5|
      Ulcer, Focal                         |                                                                          |      3  2.7|
                                            __________________________________________________________________________|____________|
   Stomach, Glandular                      |                                                                          |  50        |
      Erosion, Focal                       |                                                                          |      2  2.0|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  73                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                                            |      A     |
    1000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
                                           |                                                                          |            |
      Inflammation, Acute, Focal           |                                                                          |      1  2.0|
      Ulcer, Focal                         |                                                                          |      3  2.0|
                                            __________________________________________________________________________|____________|
   Tooth                                   |                                                                          |   1        |
      Gingiva, Inflammation, Chronic Active|                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Heart                                   |                                                                          |  50        |
      Cardiomyopathy                       |                                                                          |     31  1.7|
      Atrium, Dilatation                   |                                                                          |      3  3.0|
      Atrium, Thrombosis                   |                                                                          |      2  2.0|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Adrenal Cortex                          |                                                                          |  50        |
      Angiectasis, Focal                   |                                                                          |      1  2.0|
      Congestion                           |                                                                          |      1  1.0|
      Cyst                                 |                                                                          |      1  3.0|
      Degeneration, Cystic, Focal          |                                                                          |      2  1.5|
      Hyperplasia, Focal                   |                                                                          |      6  1.5|
      Vacuolization Cytoplasmic, Focal     |                                                                          |     12  1.9|
                                            __________________________________________________________________________|____________|
   Adrenal Medulla                         |                                                                          |  50        |
      Hyperplasia                          |                                                                          |     12  1.8|
      Bilateral, Hyperplasia               |                                                                          |      3  2.0|
                                            __________________________________________________________________________|____________|
   Islets, Pancreatic                      |                                                                          |  50        |
      Hyperplasia, Focal                   |                                                                          |      4  2.0|
                                            __________________________________________________________________________|____________|
   Parathyroid Gland                       |                                                                          |  47        |
                                            __________________________________________________________________________|____________|
   Pituitary Gland                         |                                                                          |  50        |
      Angiectasis, Focal                   |                                                                          |      1  2.0|
      Pigmentation, Focal                  |                                                                          |      1  2.0|
      Pars Distalis, Cyst                  |                                                                          |      2  2.5|
      Pars Distalis, Hyperplasia, Focal    |                                                                          |     16  1.9|
                                            __________________________________________________________________________|____________|
   Thyroid Gland                           |                                                                          |  50        |
      C-Cell, Hyperplasia, Focal           |                                                                          |      6  2.2|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Coagulating Gland                       |                                                                          |   1        |
                                            __________________________________________________________________________|____________|
   Epididymis                              |                                                                          |  50        |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  74                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                                            |      A     |
    1000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 GENITAL SYSTEM - cont                     |                                                                          |            |
                                           |                                                                          |            |
      Degeneration, Focal                  |                                                                          |     38  2.1|
                                            __________________________________________________________________________|____________|
   Preputial Gland                         |                                                                          |  50        |
      Hyperplasia                          |                                                                          |      1  3.0|
      Inflammation, Suppurative            |                                                                          |      3  3.0|
      Duct, Dilatation                     |                                                                          |     17  2.4|
                                            __________________________________________________________________________|____________|
   Prostate                                |                                                                          |  50        |
      Abscess                              |                                                                          |      1  3.0|
      Atrophy                              |                                                                          |      7  2.0|
      Degeneration, Focal                  |                                                                          |      1  1.0|
      Inflammation, Chronic, Focal         |                                                                          |      1  3.0|
      Inflammation, Focal, Suppurative     |                                                                          |      2  2.0|
                                            __________________________________________________________________________|____________|
   Seminal Vesicle                         |                                                                          |  50        |
      Hyperplasia, Focal                   |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Testes                                  |                                                                          |  50        |
      Atrophy                              |                                                                          |      3  3.0|
      Mineralization, Focal                |                                                                          |      1  2.0|
      Bilateral, Atrophy                   |                                                                          |      2  2.0|
      Interstitial Cell, Hyperplasia, Focal|                                                                          |      2  2.0|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Blood                                   |                                                                          |   1        |
                                            __________________________________________________________________________|____________|
   Bone Marrow                             |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Lymph Node                              |                                                                          |  16        |
      Bronchial, Infiltration Cellular,    |                                                                          |            |
           Histiocyte                      |                                                                          |      1  3.0|
      Mediastinal, Hemorrhage              |                                                                          |      2  3.0|
      Renal, Hemorrhage                    |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Lymph Node, Mandibular                  |                                                                          |  50        |
      Cyst                                 |                                                                          |      2  2.0|
      Hemorrhage                           |                                                                          |      2  2.5|
      Hyperplasia, Plasma Cell             |                                                                          |     10  2.3|
                                            __________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  |                                                                          |  50        |
      Cyst                                 |                                                                          |      1  3.0|
      Hemorrhage                           |                                                                          |      2  3.0|
                                            __________________________________________________________________________|____________|
   Spleen                                  |                                                                          |  50        |
      Depletion Cellular                   |                                                                          |      1  3.0|
      Fibrosis, Focal                      |                                                                          |      1  2.0|
      Hematopoietic Cell Proliferation     |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Thymus                                  |                                                                          |  40        |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  75                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                                            |      A     |
    1000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
                                           |                                                                          |            |
      Hemorrhage                           |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Mammary Gland                           |                                                                          |  49        |
      Galactocele                          |                                                                          |      7  2.3|
      Hyperplasia, Focal                   |                                                                          |      2  2.0|
      Inflammation, Acute, Focal           |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Skin                                    |                                                                          |  50        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Bone                                    |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Skeletal Muscle                         |                                                                          |   1        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Brain                                   |                                                                          |  50        |
      Hydrocephalus                        |                                                                          |      4  2.3|
      Necrosis, Focal                      |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Lung                                    |                                                                          |  50        |
      Congestion                           |                                                                          |      1  2.0|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                                                                          |      3  1.0|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Histiocyte                       |                                                                          |      4  1.0|
      Inflammation, Focal                  |                                                                          |      2  1.5|
      Alveolar Epithelium, Hyperplasia,    |                                                                          |            |
           Focal                           |                                                                          |      1  1.0|
                                            __________________________________________________________________________|____________|
   Nose                                    |                                                                          |  50        |
      Foreign Body                         |                                                                          |      2  2.5|
      Fungus                               |                                                                          |      6  2.5|
      Hemorrhage                           |                                                                          |      1  3.0|
      Inflammation, Acute                  |                                                                          |      2  2.0|
      Inflammation, Chronic Active         |                                                                          |     10  2.8|
      Inflammation, Subacute               |                                                                          |      1  2.0|
      Olfactory Epithelium, Necrosis, Focal|                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Trachea                                 |                                                                          |  50        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |                                                                          |            |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  76                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                                            |      A     |
    1000 PPM                               | 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 SPECIAL SENSES SYSTEM - cont              |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Eye                                     |                                                                          |   4        |
      Inflammation, Chronic                |                                                                          |      1  3.0|
      Phthisis Bulbi                       |                                                                          |      1  3.0|
      Lens, Cataract                       |                                                                          |      1  4.0|
      Retina, Atrophy                      |                                                                          |      2  3.0|
                                            __________________________________________________________________________|____________|
   Harderian Gland                         |                                                                          |   1        |
                                            __________________________________________________________________________|____________|
   Zymbal's Gland                          |                                                                          |  48        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Kidney                                  |                                                                          |  50        |
      Nephropathy                          |                                                                          |     50  2.4|
      Pigmentation, Focal                  |                                                                          |     11  2.6|
                                            __________________________________________________________________________|____________|
   Urinary Bladder                         |                                                                          |  50        |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  77                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 5| 6| 7| 6| 5| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 6| 7| 5| 7| 7| 7|             
                             DAY ON TEST   | 2| 8| 2| 8| 7| 2| 7| 3| 2| 2| 2| 2| 2| 2| 3| 9| 2| 2| 2| 8| 3| 3| 3| 3| 3|             
                                           | 9| 6| 9| 1| 1| 9| 7| 7| 9| 9| 9| 9| 9| 9| 2| 2| 9| 9| 9| 0| 0| 3| 0| 0| 0|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    3000 PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal, Lymphoid         |                                                          2               |             
                                            __________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  A  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   | 2                                   1  2                                 |             
      Basophilic Focus                     | X  X  X     X  X  X     X  X           X     X  X  X  X  X  X  X     X  X|             
      Clear Cell Focus                     | X     X        X        X        X                    X     X        X  X|             
      Congestion                           |    2                                                                     |             
      Degeneration, Cystic, Focal          |          1  1     2           1              1                    2      |             
      Eosinophilic Focus                   |       X           X        X  X     X        X        X     X     X     X|             
      Fatty Change, Diffuse                |                                                                          |             
      Fatty Change, Focal                  |             1     2           1        1           1  1  1               |             
      Hyperplasia, Focal                   |                                                                          |             
      Inflammation, Chronic, Focal         |                                  1     1                                 |             
      Necrosis, Focal                      |                                                                          |             
      Bile Duct, Cyst                      |                                                                          |             
      Bile Duct, Hyperplasia, Focal        |                                  3  3                                    |             
                                            __________________________________________________________________________|             
   Mesentery                               |                   +                                         +            |             
      Fat, Necrosis, Focal                 |                   3                                         3            |             
                                            __________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Acute, Focal           |                         2                                                |             
      Acinus, Atrophy, Diffuse             | 4                                                                        |             
      Acinus, Atrophy, Focal               |    2  2  2  2  2     2  3           3        1  1     1  1  2  2  3      |             
      Acinus, Hyperplasia, Focal           |       2                                                                  |             
      Artery, Inflammation, Focal          |                                                                          |             
                                            __________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Basal Cell, Focal       |       1                    1           1                             1  1|             
      Hyperplasia, Focal, Squamous         |    2     1                    2                    1                     |             
      Inflammation, Chronic Active, Focal  |             2                                                            |             
      Ulcer, Focal                         |             1                 2                                          |             
                                            __________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
      Hyperplasia, Basal Cell, Focal       |                                                 4                        |             
                                            __________________________________________________________________________|             
   Tooth                                   |                                                                          |             
      Peridontal Tissue, Inflammation,     |                                                                          |             
           Focal                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  78                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 5| 6| 7| 6| 5| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 6| 7| 5| 7| 7| 7|             
                             DAY ON TEST   | 2| 8| 2| 8| 7| 2| 7| 3| 2| 2| 2| 2| 2| 2| 3| 9| 2| 2| 2| 8| 3| 3| 3| 3| 3|             
                                           | 9| 6| 9| 1| 1| 9| 7| 7| 9| 9| 9| 9| 9| 9| 2| 2| 9| 9| 9| 0| 0| 3| 0| 0| 0|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    3000 PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM - cont              |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       |    2  2  2  2  3  2  1  2     2  2     2  2     2  2  2  2  2     2  2  3|             
      Mineralization, Focal                |                                                                          |             
      Atrium, Dilatation                   |                   3                                      3               |             
      Atrium, Thrombosis                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   | 2                       2           2              1                     |             
      Vacuolization Cytoplasmic, Focal     |                                                    2                     |             
                                            __________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                       2                  |             
      Hyperplasia                          |    2           1        2        2        2     1        3              3|             
      Bilateral, Hyperplasia               |                                                    2                     |             
                                            __________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   | 3                          1     1                                       |             
                                            __________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  M  +  +  +  +  +  +  M  +  +  +  +  +  +  M  +  +  +  +  +  +|             
      Hyperplasia                          |                                                                          |             
                                            __________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +|             
      Pars Distalis, Cyst                  |                      X                                         X         |             
      Pars Distalis, Hyperplasia, Focal    |       3        1                 1                             2         |             
                                            __________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      C-Cell, Hyperplasia, Focal           |                               3                 1  2        2            |             
      Follicular Cell, Hyperplasia, Focal  |                            1                                             |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration, Focal                  | 2  2  1  3  2  2  3     2  2  2  2  2        2  2     2     2        2  2|             
                                            __________________________________________________________________________|             
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                                       2                  |             
      Inflammation, Suppurative            |                                                                          |             
      Duct, Dilatation                     | 2     2                                   2  2     2                     |             
                                            __________________________________________________________________________|             
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |       2        3        2  2  2           3                              |             
      Hyperplasia, Focal                   |                                  2              1                        |             
      Inflammation, Chronic, Focal         |                                                                          |             
      Inflammation, Focal, Granulomatous   |    1                                                     2               |             
      Inflammation, Focal, Suppurative     |                                                    2                    2|             
                                            __________________________________________________________________________|             
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Dilatation                           |                                                                          |             
                                            __________________________________________________________________________|             
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  79                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 5| 6| 7| 6| 5| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 6| 7| 5| 7| 7| 7|             
                             DAY ON TEST   | 2| 8| 2| 8| 7| 2| 7| 3| 2| 2| 2| 2| 2| 2| 3| 9| 2| 2| 2| 8| 3| 3| 3| 3| 3|             
                                           | 9| 6| 9| 1| 1| 9| 7| 7| 9| 9| 9| 9| 9| 9| 2| 2| 9| 9| 9| 0| 0| 3| 0| 0| 0|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    3000 PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM - cont                     |                                                                          |             
                                           |                                                                          |             
      Atrophy                              |    3                                            2     3                  |             
      Cyst                                 |       2                          3                                       |             
      Artery, Inflammation, Focal          |                                                    2                     |             
      Interstitial Cell, Hyperplasia, Focal|                                                                          |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |                                                    2                     |             
      Hyperplasia                          |                         2                                            2   |             
                                            __________________________________________________________________________|             
   Lymph Node                              | +        +     +     +  +        +  +  +  +  +                    +      |             
      Lumbar, Hemorrhage                   |                         1                                                |             
      Lumbar, Infiltration Cellular,       |                                                                          |             
          Histiocyte                       |                                        2                                 |             
      Mediastinal, Cyst                    | 2                                                                        |             
      Mediastinal, Hemorrhage              |                         2                 2                              |             
      Mediastinal, Hyperplasia, Plasma Cell|                                                                          |             
      Mediastinal, Infiltration Cellular,  |                                                                          |             
           Histiocyte                      |                                                                          |             
      Pancreatic, Hemorrhage               |                                           2                              |             
      Pancreatic, Infiltration Cellular,   |                                                                          |             
           Histiocyte                      |                                  3                                       |             
      Renal, Hemorrhage                    |                                                                          |             
                                            __________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 | 2                       2                                            2   |             
      Hemorrhage                           |                   1                                                      |             
      Hyperplasia, Plasma Cell             |    2                                                           3         |             
                                            __________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Edema                                |                                                          3               |             
      Hemorrhage                           |                                                       1                  |             
                                            __________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |             2                                                            |             
      Fibrosis, Focal                      |                                  3     2                                 |             
                                            __________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Galactocele                          |                                                    3                     |             
      Hyperplasia, Focal                   |                                     2           1                        |             
                                            __________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acanthosis, Focal                    |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Fibrous Osteodystrophy               |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  80                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 6| 7| 5| 6| 7| 6| 5| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 6| 7| 5| 7| 7| 7|             
                             DAY ON TEST   | 2| 8| 2| 8| 7| 2| 7| 3| 2| 2| 2| 2| 2| 2| 3| 9| 2| 2| 2| 8| 3| 3| 3| 3| 3|             
                                           | 9| 6| 9| 1| 1| 9| 7| 7| 9| 9| 9| 9| 9| 9| 2| 2| 9| 9| 9| 0| 0| 3| 0| 0| 0|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|             
    3000 PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM - cont             |                                                                          |             
                                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hydrocephalus                        |                                                                          |             
      Mineralization, Focal                |                                                                          |             
                                            __________________________________________________________________________|             
   Spinal Cord                             |             +                                                            |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                   3                                                      |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                                                                      2   |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Histiocyte                       |                                              3     1                     |             
      Inflammation, Focal                  |                         2                                1  2        2   |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                                                                         2|             
                                            __________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                   3                                                      |             
      Foreign Body                         |    3                                                           2         |             
      Fungus                               | 3     3                 3                             2        3  2  3   |             
      Hemorrhage                           |                                                                          |             
      Inflammation, Acute                  |                                                                          |             
      Inflammation, Chronic Active         | 3  3  3                 3                             2        3  2  3   |             
      Inflammation, Subacute               |                1                          2                              |             
                                            __________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic Active, Focal  |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Eye                                     |       +                 +        +                                       |             
      Cornea, Degeneration, Focal          |                                  3                                       |             
      Lens, Cataract                       |       3                                                                  |             
                                            __________________________________________________________________________|             
   Zymbal's Gland                          | +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
      Hydronephrosis                       |                                                    4                     |             
      Nephropathy                          | 4  3  3  2  2  3  2  2  3  3  2  3  3  4  1  2  4  4  3  1  3  1  3  4  3|             
      Pigmentation, Focal                  |             2        1              2        2  1        1  1            |             
                                            __________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  81                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 3| 7| 7| 7| 7| 6| 6| 6|             
                             DAY ON TEST   | 7| 0| 2| 3| 3| 9| 3| 3| 3| 3| 3| 1| 0| 3| 3| 3| 7| 5| 2| 3| 3| 3| 9| 4| 7|             
                                           | 0| 0| 1| 0| 0| 2| 0| 0| 0| 0| 0| 4| 9| 0| 0| 0| 4| 6| 5| 0| 0| 0| 4| 9| 7|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    3000 PPM                               | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal, Lymphoid         |                                                                          |             
                                            __________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                                                             2  2  2  2   |             
      Basophilic Focus                     |          X  X     X  X  X  X  X     X  X  X  X  X     X     X  X  X      |             
      Clear Cell Focus                     |          X  X     X  X  X  X  X           X  X                    X      |             
      Congestion                           |                                                    2  3                 4|             
      Degeneration, Cystic, Focal          |    2  2  1        2  2  1  2           2     2           3  3     1      |             
      Eosinophilic Focus                   |                            X           X        X        X  X            |             
      Fatty Change, Diffuse                |                                                                      3   |             
      Fatty Change, Focal                  |       3     1                    1        2                    1         |             
      Hyperplasia, Focal                   |                                                          3               |             
      Inflammation, Chronic, Focal         |          1                                   1  1                        |             
      Necrosis, Focal                      |                                     2                                    |             
      Bile Duct, Cyst                      |                                                          1     1         |             
      Bile Duct, Hyperplasia, Focal        | 3  3  3                                                  3  2  2  3      |             
                                            __________________________________________________________________________|             
   Mesentery                               |                      +              +                    +        +  +   |             
      Fat, Necrosis, Focal                 |                      3                                   2        2      |             
                                            __________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Acute, Focal           |                                                                          |             
      Acinus, Atrophy, Diffuse             |                                                                          |             
      Acinus, Atrophy, Focal               |       2        2  1     1  3           2  2  1     1  1  2  1  2  2      |             
      Acinus, Hyperplasia, Focal           |                                                                          |             
      Artery, Inflammation, Focal          |                   2                                                      |             
                                            __________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Basal Cell, Focal       | 1                          1        1           1     1                  |             
      Hyperplasia, Focal, Squamous         |                                                       2              3   |             
      Inflammation, Chronic Active, Focal  |                                                                      2   |             
      Ulcer, Focal                         |                                                                      3   |             
                                            __________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                      2   |             
      Hyperplasia, Basal Cell, Focal       |                                                                          |             
                                            __________________________________________________________________________|             
   Tooth                                   |    +                                                                     |             
      Peridontal Tissue, Inflammation,     |                                                                          |             
           Focal                           |    3                                                                     |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  82                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 3| 7| 7| 7| 7| 6| 6| 6|             
                             DAY ON TEST   | 7| 0| 2| 3| 3| 9| 3| 3| 3| 3| 3| 1| 0| 3| 3| 3| 7| 5| 2| 3| 3| 3| 9| 4| 7|             
                                           | 0| 0| 1| 0| 0| 2| 0| 0| 0| 0| 0| 4| 9| 0| 0| 0| 4| 6| 5| 0| 0| 0| 4| 9| 7|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    3000 PPM                               | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM - cont              |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 2  2     2  2  2     1     2  1     1  2  2  2  2     3     2  2  2     2|             
      Mineralization, Focal                |                                                       2                  |             
      Atrium, Dilatation                   |                                                       3                  |             
      Atrium, Thrombosis                   |                                  3                                       |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |          2                                                  1            |             
      Vacuolization Cytoplasmic, Focal     |                                                 2                    2   |             
                                            __________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                                          |             
      Hyperplasia                          |                         2        2              2  1  2                 2|             
      Bilateral, Hyperplasia               |             2        3                                                   |             
                                            __________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   | 2                       1  2     2                                       |             
                                            __________________________________________________________________________|             
   Parathyroid Gland                       | +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                           2           3                  |             
                                            __________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +|             
      Pars Distalis, Cyst                  |    3                                                                     |             
      Pars Distalis, Hyperplasia, Focal    |       3                 1                    3  2     2  3  3            |             
                                            __________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      C-Cell, Hyperplasia, Focal           |       2           2        2              2                 2  1         |             
      Follicular Cell, Hyperplasia, Focal  |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration, Focal                  | 2  3     1  2     2  2     2  1  2  1  2  1     1     3     2  2  2  3  2|             
                                            __________________________________________________________________________|             
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                        2                                 |             
      Inflammation, Suppurative            |          3                 2                                             |             
      Duct, Dilatation                     |       2        2                                         2           3   |             
                                            __________________________________________________________________________|             
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |          2  2           1     2     2                                    |             
      Hyperplasia, Focal                   |          2                                                               |             
      Inflammation, Chronic, Focal         |                            2                 2                           |             
      Inflammation, Focal, Granulomatous   |    2                                                                     |             
      Inflammation, Focal, Suppurative     |                               2                    2                     |             
                                            __________________________________________________________________________|             
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Dilatation                           |                                           3                              |             
                                            __________________________________________________________________________|             
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  83                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 3| 7| 7| 7| 7| 6| 6| 6|             
                             DAY ON TEST   | 7| 0| 2| 3| 3| 9| 3| 3| 3| 3| 3| 1| 0| 3| 3| 3| 7| 5| 2| 3| 3| 3| 9| 4| 7|             
                                           | 0| 0| 1| 0| 0| 2| 0| 0| 0| 0| 0| 4| 9| 0| 0| 0| 4| 6| 5| 0| 0| 0| 4| 9| 7|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    3000 PPM                               | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM - cont                     |                                                                          |             
                                           |                                                                          |             
      Atrophy                              |                                                             3            |             
      Cyst                                 |                                                                          |             
      Artery, Inflammation, Focal          |                                                                          |             
      Interstitial Cell, Hyperplasia, Focal|                                                             1            |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |                                                                          |             
      Hyperplasia                          |                                                       3                  |             
                                            __________________________________________________________________________|             
   Lymph Node                              | +     +        +        +        +     +     +  +     +  +  +        +   |             
      Lumbar, Hemorrhage                   |                                                                          |             
      Lumbar, Infiltration Cellular,       |                                                                          |             
          Histiocyte                       |                                              3                           |             
      Mediastinal, Cyst                    |                                                                          |             
      Mediastinal, Hemorrhage              |                         1                       3     3                  |             
      Mediastinal, Hyperplasia, Plasma Cell|                                                 2                        |             
      Mediastinal, Infiltration Cellular,  |                                                                          |             
           Histiocyte                      |                                              2                           |             
      Pancreatic, Hemorrhage               |                                                                          |             
      Pancreatic, Infiltration Cellular,   |                                                                          |             
           Histiocyte                      |                                                                          |             
      Renal, Hemorrhage                    |                                                 3                        |             
                                            __________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +|             
      Cyst                                 |             2           3     2                                          |             
      Hemorrhage                           |                                                    1  1                  |             
      Hyperplasia, Plasma Cell             |                2                          2                              |             
                                            __________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Edema                                |                                                                          |             
      Hemorrhage                           |                                                                          |             
                                            __________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                                          |             
      Fibrosis, Focal                      |                                                 1                 2      |             
                                            __________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Galactocele                          |                                                                          |             
      Hyperplasia, Focal                   |                                                                2         |             
                                            __________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acanthosis, Focal                    |                                                                2         |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Fibrous Osteodystrophy               |                                                       1                  |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  84                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 6| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 3| 7| 7| 7| 7| 6| 6| 6|             
                             DAY ON TEST   | 7| 0| 2| 3| 3| 9| 3| 3| 3| 3| 3| 1| 0| 3| 3| 3| 7| 5| 2| 3| 3| 3| 9| 4| 7|             
                                           | 0| 0| 1| 0| 0| 2| 0| 0| 0| 0| 0| 4| 9| 0| 0| 0| 4| 6| 5| 0| 0| 0| 4| 9| 7|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|             
    3000 PPM                               | 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM - cont             |                                                                          |             
                                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hydrocephalus                        |                                     2                                    |             
      Mineralization, Focal                |                                                    2                     |             
                                            __________________________________________________________________________|             
   Spinal Cord                             |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                                          |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Lymphocyte                       |                   1     1                                                |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Histiocyte                       |                   1                             1        1        1      |             
      Inflammation, Focal                  |             1     1                                   1                  |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                      2                          2                        |             
                                            __________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                                          |             
      Foreign Body                         |                                                                      2   |             
      Fungus                               |             3     3  3  3     3              3  3  3  3                  |             
      Hemorrhage                           |                            3                                             |             
      Inflammation, Acute                  |                      3                                                   |             
      Inflammation, Chronic Active         |    3        3     4     3     3     2     3  4  3  3  3              2   |             
      Inflammation, Subacute               |                                                                         2|             
                                            __________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic Active, Focal  |             2                                                            |             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Eye                                     |                                                                          |             
      Cornea, Degeneration, Focal          |                                                                          |             
      Lens, Cataract                       |                                                                          |             
                                            __________________________________________________________________________|             
   Zymbal's Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                               2                       3                  |             
      Hydronephrosis                       |                                                                          |             
      Nephropathy                          | 3  3  2  3  4  2  4  3  3  3  4  3  3  3  4  3  3  1  4  2  3  3  2  3  2|             
      Pigmentation, Focal                  | 3  3  3        2                    2        1              1     1      |             
                                            __________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
                                                             Page  85                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                                            |      A     |
    3000 PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Esophagus                               |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Colon                  |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Rectum                 |                                                                          |  49        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Cecum                  |                                                                          |  49        |
      Hyperplasia, Focal, Lymphoid         |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Intestine Small, Duodenum               |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Jejunum                |                                                                          |  49        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Ileum                  |                                                                          |  48        |
                                            __________________________________________________________________________|____________|
   Liver                                   |                                                                          |  50        |
      Angiectasis, Focal                   |                                                                          |      7  1.9|
      Basophilic Focus                     |                                                                          |     34     |
      Clear Cell Focus                     |                                                                          |     19     |
      Congestion                           |                                                                          |      4  2.8|
      Degeneration, Cystic, Focal          |                                                                          |     18  1.7|
      Eosinophilic Focus                   |                                                                          |     15     |
      Fatty Change, Diffuse                |                                                                          |      1  3.0|
      Fatty Change, Focal                  |                                                                          |     12  1.3|
      Hyperplasia, Focal                   |                                                                          |      1  3.0|
      Inflammation, Chronic, Focal         |                                                                          |      5  1.0|
      Necrosis, Focal                      |                                                                          |      1  2.0|
      Bile Duct, Cyst                      |                                                                          |      2  1.0|
      Bile Duct, Hyperplasia, Focal        |                                                                          |      9  2.8|
                                            __________________________________________________________________________|____________|
   Mesentery                               |                                                                          |   7        |
      Fat, Necrosis, Focal                 |                                                                          |      5  2.6|
                                            __________________________________________________________________________|____________|
   Pancreas                                |                                                                          |  50        |
      Inflammation, Acute, Focal           |                                                                          |      1  2.0|
      Acinus, Atrophy, Diffuse             |                                                                          |      1  4.0|
      Acinus, Atrophy, Focal               |                                                                          |     29  1.8|
      Acinus, Hyperplasia, Focal           |                                                                          |      1  2.0|
      Artery, Inflammation, Focal          |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Salivary Glands                         |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Stomach, Forestomach                    |                                                                          |  50        |
      Hyperplasia, Basal Cell, Focal       |                                                                          |     10  1.0|
      Hyperplasia, Focal, Squamous         |                                                                          |      6  1.8|
      Inflammation, Chronic Active, Focal  |                                                                          |      2  2.0|
      Ulcer, Focal                         |                                                                          |      3  2.0|
                                            __________________________________________________________________________|____________|
   Stomach, Glandular                      |                                                                          |  50        |
      Cyst                                 |                                                                          |      1  2.0|
      Hyperplasia, Basal Cell, Focal       |                                                                          |      1  4.0|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  86                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                                            |      A     |
    3000 PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Tooth                                   |                                                                          |   1        |
      Peridontal Tissue, Inflammation,     |                                                                          |            |
           Focal                           |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Heart                                   |                                                                          |  50        |
      Cardiomyopathy                       |                                                                          |     38  2.0|
      Mineralization, Focal                |                                                                          |      1  2.0|
      Atrium, Dilatation                   |                                                                          |      3  3.0|
      Atrium, Thrombosis                   |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Adrenal Cortex                          |                                                                          |  50        |
      Hyperplasia, Focal                   |                                                                          |      6  1.7|
      Vacuolization Cytoplasmic, Focal     |                                                                          |      3  2.0|
                                            __________________________________________________________________________|____________|
   Adrenal Medulla                         |                                                                          |  50        |
      Cyst                                 |                                                                          |      1  2.0|
      Hyperplasia                          |                                                                          |     14  1.9|
      Bilateral, Hyperplasia               |                                                                          |      3  2.3|
                                            __________________________________________________________________________|____________|
   Islets, Pancreatic                      |                                                                          |  50        |
      Hyperplasia, Focal                   |                                                                          |      7  1.7|
                                            __________________________________________________________________________|____________|
   Parathyroid Gland                       |                                                                          |  46        |
      Hyperplasia                          |                                                                          |      2  2.5|
                                            __________________________________________________________________________|____________|
   Pituitary Gland                         |                                                                          |  48        |
      Pars Distalis, Cyst                  |                                                                          |      3  3.0|
      Pars Distalis, Hyperplasia, Focal    |                                                                          |     11  2.2|
                                            __________________________________________________________________________|____________|
   Thyroid Gland                           |                                                                          |  50        |
      C-Cell, Hyperplasia, Focal           |                                                                          |     10  1.9|
      Follicular Cell, Hyperplasia, Focal  |                                                                          |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Epididymis                              |                                                                          |  50        |
      Degeneration, Focal                  |                                                                          |     37  2.0|
                                            __________________________________________________________________________|____________|
   Preputial Gland                         |                                                                          |  50        |
      Hyperplasia                          |                                                                          |      2  2.0|
      Inflammation, Suppurative            |                                                                          |      2  2.5|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  87                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                                            |      A     |
    3000 PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 GENITAL SYSTEM - cont                     |                                                                          |            |
                                           |                                                                          |            |
      Duct, Dilatation                     |                                                                          |      9  2.1|
                                            __________________________________________________________________________|____________|
   Prostate                                |                                                                          |  50        |
      Atrophy                              |                                                                          |     11  2.1|
      Hyperplasia, Focal                   |                                                                          |      3  1.7|
      Inflammation, Chronic, Focal         |                                                                          |      2  2.0|
      Inflammation, Focal, Granulomatous   |                                                                          |      3  1.7|
      Inflammation, Focal, Suppurative     |                                                                          |      4  2.0|
                                            __________________________________________________________________________|____________|
   Seminal Vesicle                         |                                                                          |  50        |
      Dilatation                           |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Testes                                  |                                                                          |  50        |
      Atrophy                              |                                                                          |      4  2.8|
      Cyst                                 |                                                                          |      2  2.5|
      Artery, Inflammation, Focal          |                                                                          |      1  2.0|
      Interstitial Cell, Hyperplasia, Focal|                                                                          |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Bone Marrow                             |                                                                          |  50        |
      Atrophy                              |                                                                          |      1  2.0|
      Hyperplasia                          |                                                                          |      3  2.3|
                                            __________________________________________________________________________|____________|
   Lymph Node                              |                                                                          |  23        |
      Lumbar, Hemorrhage                   |                                                                          |      1  1.0|
      Lumbar, Infiltration Cellular,       |                                                                          |            |
          Histiocyte                       |                                                                          |      2  2.5|
      Mediastinal, Cyst                    |                                                                          |      1  2.0|
      Mediastinal, Hemorrhage              |                                                                          |      5  2.2|
      Mediastinal, Hyperplasia, Plasma Cell|                                                                          |      1  2.0|
      Mediastinal, Infiltration Cellular,  |                                                                          |            |
           Histiocyte                      |                                                                          |      1  2.0|
      Pancreatic, Hemorrhage               |                                                                          |      1  2.0|
      Pancreatic, Infiltration Cellular,   |                                                                          |            |
           Histiocyte                      |                                                                          |      1  3.0|
      Renal, Hemorrhage                    |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Lymph Node, Mandibular                  |                                                                          |  49        |
      Cyst                                 |                                                                          |      6  2.2|
      Hemorrhage                           |                                                                          |      3  1.0|
      Hyperplasia, Plasma Cell             |                                                                          |      4  2.3|
                                            __________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  |                                                                          |  50        |
      Edema                                |                                                                          |      1  3.0|
      Hemorrhage                           |                                                                          |      1  1.0|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  88                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                                            |      A     |
    3000 PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Spleen                                  |                                                                          |  50        |
      Congestion                           |                                                                          |      1  2.0|
      Fibrosis, Focal                      |                                                                          |      4  2.0|
                                            __________________________________________________________________________|____________|
   Thymus                                  |                                                                          |  49        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Mammary Gland                           |                                                                          |  50        |
      Galactocele                          |                                                                          |      1  3.0|
      Hyperplasia, Focal                   |                                                                          |      3  1.7|
                                            __________________________________________________________________________|____________|
   Skin                                    |                                                                          |  50        |
      Acanthosis, Focal                    |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Bone                                    |                                                                          |  50        |
      Fibrous Osteodystrophy               |                                                                          |      1  1.0|
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Brain                                   |                                                                          |  50        |
      Hydrocephalus                        |                                                                          |      1  2.0|
      Mineralization, Focal                |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Spinal Cord                             |                                                                          |   1        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Lung                                    |                                                                          |  50        |
      Congestion                           |                                                                          |      1  3.0|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Lymphocyte                       |                                                                          |      3  1.3|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Histiocyte                       |                                                                          |      6  1.3|
      Inflammation, Focal                  |                                                                          |      7  1.4|
      Alveolar Epithelium, Hyperplasia,    |                                                                          |            |
           Focal                           |                                                                          |      3  2.0|
                                            __________________________________________________________________________|____________|
   Nose                                    |                                                                          |  49        |
      Congestion                           |                                                                          |      1  3.0|
      Foreign Body                         |                                                                          |      3  2.3|
      Fungus                               |                                                                          |     16  2.9|
      Hemorrhage                           |                                                                          |      1  3.0|
      Inflammation, Acute                  |                                                                          |      1  3.0|
      Inflammation, Chronic Active         |                                                                          |     20  2.9|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  89                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3|                                            |      A     |
    3000 PPM                               | 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 RESPIRATORY SYSTEM - cont                 |                                                                          |            |
                                           |                                                                          |            |
      Inflammation, Subacute               |                                                                          |      3  1.7|
                                            __________________________________________________________________________|____________|
   Trachea                                 |                                                                          |  50        |
      Inflammation, Chronic Active, Focal  |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Eye                                     |                                                                          |   3        |
      Cornea, Degeneration, Focal          |                                                                          |      1  3.0|
      Lens, Cataract                       |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Zymbal's Gland                          |                                                                          |  48        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Kidney                                  |                                                                          |  50        |
      Cyst                                 |                                                                          |      2  2.5|
      Hydronephrosis                       |                                                                          |      1  4.0|
      Nephropathy                          |                                                                          |     50  2.8|
      Pigmentation, Focal                  |                                                                          |     15  1.7|
                                            __________________________________________________________________________|____________|
   Urinary Bladder                         |                                                                          |  50        |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page  90                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 5| 7| 5| 3| 7| 7| 6| 7| 6| 4| 7| 7| 7| 6| 7| 6| 6| 5| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 8| 2| 0| 2| 6| 0| 2| 2| 4| 2| 9| 6| 2| 2| 2| 4| 2| 8| 8| 8| 2| 2| 0| 2| 2|             
                                           | 0| 9| 6| 9| 6| 5| 9| 9| 6| 9| 9| 0| 9| 2| 9| 6| 9| 0| 4| 3| 9| 9| 2| 9| 9|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|             
    10000PPM                               | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                                                                         2|             
      Basophilic Focus                     |       X  X        X  X     X        X     X     X  X        X  X        X|             
      Clear Cell Focus                     |          X        X  X     X        X     X     X           X  X        X|             
      Congestion                           |                                  2                                       |             
      Degeneration, Cystic, Focal          |    1     2        2     1  2  2     2  3  2              1  2           2|             
      Eosinophilic Focus                   | X  X  X  X                 X              X  X           X  X  X     X  X|             
      Fatty Change, Focal                  |                            1  1  2  2     1        1        2        1   |             
      Hepatodiaphragmatic Nodule           |                                              X                           |             
      Inflammation, Chronic, Focal         |                   1                                               2      |             
      Mixed Cell Focus                     |                   X  X              X                             X  X   |             
      Necrosis, Focal                      |                2                 2                                3      |             
      Pigmentation, Focal                  |                                                                          |             
      Bile Duct, Cyst                      |                                                                          |             
      Bile Duct, Fibrosis                  |                                                                   4      |             
      Bile Duct, Hyperplasia, Focal        | 3                             3                                          |             
      Centrilobular, Atrophy, Focal        |                2                                                         |             
      Vein, Dilatation                     |                                                                          |             
                                            __________________________________________________________________________|             
   Mesentery                               |                         +     +                                   +      |             
      Fat, Necrosis, Focal                 |                         3     3                                   3      |             
                                            __________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acinus, Atrophy, Diffuse             |                                                                          |             
      Acinus, Atrophy, Focal               | 2  1     1           1  1     1     2     1  2  2  2  2     2  3  1  1   |             
      Acinus, Cytoplasmic Alteration, Focal|                                  2                                       |             
                                            __________________________________________________________________________|             
   Pharynx                                 |    +                       +                          +     +  +         |             
      Palate, Hyperplasia, Focal, Squamous |    4                                                                     |             
                                            __________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |                                                                          |             
                                            __________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acanthosis, Focal                    |          1                                                               |             
      Hyperplasia, Basal Cell, Focal       |                   2  1     1  2     1        1        2     1  2  2  1  2|             
      Hyperplasia, Focal, Plasma Cell      |                                                                   2      |             
      Hyperplasia, Focal, Squamous         |    1                       1                    2                        |             
                                            __________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Erosion, Focal                       |                               2                                          |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  91                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 5| 7| 5| 3| 7| 7| 6| 7| 6| 4| 7| 7| 7| 6| 7| 6| 6| 5| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 8| 2| 0| 2| 6| 0| 2| 2| 4| 2| 9| 6| 2| 2| 2| 4| 2| 8| 8| 8| 2| 2| 0| 2| 2|             
                                           | 0| 9| 6| 9| 6| 5| 9| 9| 6| 9| 9| 0| 9| 2| 9| 6| 9| 0| 4| 3| 9| 9| 2| 9| 9|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|             
    10000PPM                               | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM - cont              |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 2     1  1  2     2  2  2  1        2     2     2  2  1  3  3  2     2  1|             
      Mineralization, Focal                |                                                                          |             
      Atrium, Thrombosis                   | 3                                                                        |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                                          |             
      Cyst, Multiple                       |                                                                          |             
      Hemorrhage                           |                                                                          |             
      Hyperplasia, Focal                   |                                                                         2|             
      Vacuolization Cytoplasmic, Focal     |    1           2        3  3  2                             3     2      |             
                                            __________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |       2                 2  2        2                       2            |             
      Bilateral, Hyperplasia               |                                                                          |             
                                            __________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |                   4                                   3     3     2  2   |             
                                            __________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  M  +  +  +  M  +  +  +  +  M  +  +  +  +  +|             
      Bilateral, Hyperplasia               |                                                       3                  |             
                                            __________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Pars Distalis, Hyperplasia, Focal    | 3  2     1        1     2  2        1     2           2     2  1         |             
      Pars Nervosa, Hyperplasia, Focal     |                                                                          |             
                                            __________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      C-Cell, Hyperplasia, Focal           |                                     1                                    |             
      Follicle, Dilatation, Focal          |                3           2                                             |             
      Follicular Cell, Hyperplasia, Focal  |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration, Focal                  | 3  2     2        1  2  2  1  3     2  2  2  3  2     1     2  1  3  2  2|             
                                            __________________________________________________________________________|             
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                     3                                    |             
      Inflammation, Chronic                |          2                                                               |             
      Inflammation, Suppurative            |                                                                          |             
      Duct, Dilatation                     |    1                    2  3  3              3  2                        |             
                                            __________________________________________________________________________|             
   Prostate                                | +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Abscess                              |                                                                          |             
      Atrophy                              |    2     2           2        2        3                       2     2  2|             
      Inflammation, Chronic, Focal         |    2                       3        2                                    |             
      Inflammation, Focal, Granulomatous   |       2                                                                  |             
      Inflammation, Focal, Suppurative     |                         3                    3                           |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  92                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 5| 7| 5| 3| 7| 7| 6| 7| 6| 4| 7| 7| 7| 6| 7| 6| 6| 5| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 8| 2| 0| 2| 6| 0| 2| 2| 4| 2| 9| 6| 2| 2| 2| 4| 2| 8| 8| 8| 2| 2| 0| 2| 2|             
                                           | 0| 9| 6| 9| 6| 5| 9| 9| 6| 9| 9| 0| 9| 2| 9| 6| 9| 0| 4| 3| 9| 9| 2| 9| 9|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|             
    10000PPM                               | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM - cont                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Seminal Vesicle                         | +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Abscess                              |                                                                          |             
      Inflammation, Chronic, Focal         |                                                             2            |             
                                            __________________________________________________________________________|             
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              | 3                    4           3              4                        |             
      Interstitial Cell, Hyperplasia, Focal| 2     1                          2              2                        |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |       3        3                                                         |             
                                            __________________________________________________________________________|             
   Lymph Node                              |    +  +              +        +        +     +     +  +           +     +|             
      Axillary, Hyperplasia, Plasma Cell   |                                                    2                     |             
      Iliac, Cyst                          |       X                                                                  |             
      Iliac, Infiltration Cellular,        |                                                                          |             
          Histiocyte                       |       2                                                                  |             
      Inguinal, Cyst                       |       X                                                                  |             
      Lumbar, Hemorrhage                   |                      2                                                   |             
      Lumbar, Hyperplasia, Plasma Cell     |                                                                          |             
      Mediastinal, Hemorrhage              |                                                                          |             
      Mediastinal, Infiltration Cellular,  |                                                                          |             
           Histiocyte                      |                               3                                          |             
      Pancreatic, Hemorrhage               |                                                                          |             
      Renal, Hemorrhage                    |                                                       2                  |             
      Renal, Infiltration Cellular,        |                                                                          |             
          Histiocyte                       |                                                                   3      |             
                                            __________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                              3                           |             
      Hemorrhage                           |                                                                          |             
      Hyperplasia, Plasma Cell             |          2                                                              2|             
                                            __________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                         1                                                |             
                                            __________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Fibrosis, Focal                      |                            2                    2                        |             
      Hematopoietic Cell Proliferation     |                2                                                  3      |             
      Capsule, Cyst                        |                                                                          |             
                                            __________________________________________________________________________|             
   Thymus                                  | +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                                                          |             
      Hemorrhage                           |                                  2                                       |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  M  +  +  M  +  +  +  +  +|             
      Galactocele                          |                                                             3            |             
      Hyperplasia, Focal                   | 3                                                           2            |             
      Inflammation, Chronic, Focal         |                                                             3            |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  93                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 5| 7| 5| 3| 7| 7| 6| 7| 6| 4| 7| 7| 7| 6| 7| 6| 6| 5| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 8| 2| 0| 2| 6| 0| 2| 2| 4| 2| 9| 6| 2| 2| 2| 4| 2| 8| 8| 8| 2| 2| 0| 2| 2|             
                                           | 0| 9| 6| 9| 6| 5| 9| 9| 6| 9| 9| 0| 9| 2| 9| 6| 9| 0| 4| 3| 9| 9| 2| 9| 9|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|             
    10000PPM                               | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM - cont               |                                                                          |             
                                           |                                                                          |             
      Pigmentation, Focal                  |                                                                          |             
                                            __________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Subcutaneous Tissue, Inflammation,   |                                                                          |             
           Acute                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Fibrous Osteodystrophy               |                                                                          |             
      Hyperplasia, Focal                   |                                                                          |             
                                            __________________________________________________________________________|             
   Skeletal Muscle                         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Gliosis, Focal                       |                                                                          |             
      Hydrocephalus                        |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                2                 3                                       |             
      Hemorrhage, Focal                    |                                                       2                  |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Histiocyte                       |                                                       2  1     1     1   |             
      Inflammation, Focal                  |                                           1              1  2            |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |                                                                2         |             
                                            __________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |                               2                                          |             
      Fungus                               |                                              3     2  3              2   |             
      Hemorrhage                           |    2                                                                     |             
      Inflammation, Chronic Active         |                               3     2        3     2  2           2  3   |             
      Inflammation, Subacute               |          2                                                               |             
      Glands, Dilatation, Focal            |                                                                          |             
                                            __________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Eye                                     |                                                 +                        |             
      Lens, Cataract                       |                                                 2                        |             
      Retina, Atrophy                      |                                                 4                        |             
                                            __________________________________________________________________________|             
   Zymbal's Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Inflammation, Chronic Active         |                2                                                         |             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                       3                  |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  94                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 6| 7| 5| 7| 5| 3| 7| 7| 6| 7| 6| 4| 7| 7| 7| 6| 7| 6| 6| 5| 7| 7| 7| 7| 7|             
                             DAY ON TEST   | 8| 2| 0| 2| 6| 0| 2| 2| 4| 2| 9| 6| 2| 2| 2| 4| 2| 8| 8| 8| 2| 2| 0| 2| 2|             
                                           | 0| 9| 6| 9| 6| 5| 9| 9| 6| 9| 9| 0| 9| 2| 9| 6| 9| 0| 4| 3| 9| 9| 2| 9| 9|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|             
    10000PPM                               | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM - cont                     |                                                                          |             
                                           |                                                                          |             
      Infarct                              |                                                                          |             
      Mineralization, Focal                |                                                          1               |             
      Nephropathy                          | 3  3     3  2     3  2  3  3  2  2  4  2  3  2  3  2  4  2  2  3  3  3  3|             
      Pigmentation, Focal                  | 2  1  3                       2     2  3     2     2     1  1     3      |             
      Pelvis, Inflammation, Suppurative    |                2                                                         |             
                                            __________________________________________________________________________|             
   Urethra                                 |                                              +                           |             
                                            __________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Abscess                              |                3                                                         |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  95                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 5| 6| 7| 6| 7| 7| 7| 7| 6| 5| 5| 7| 7| 7| 6| 6| 7| 4| 7| 5| 7| 7|             
                             DAY ON TEST   | 2| 0| 2| 3| 0| 2| 1| 2| 2| 2| 2| 1| 3| 8| 0| 2| 2| 6| 9| 2| 5| 2| 0| 2| 2|             
                                           | 9| 2| 9| 2| 1| 9| 0| 9| 9| 9| 9| 4| 1| 0| 6| 9| 9| 8| 2| 9| 0| 2| 6| 9| 9|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|             
    10000PPM                               | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 ALIMENTARY SYSTEM                         |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  +  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  A  A  +  +|             
                                            __________________________________________________________________________|             
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  A  +  +  +  +  +  +  +  +  +  A  +  +|             
                                            __________________________________________________________________________|             
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Angiectasis, Focal                   |                               2                                          |             
      Basophilic Focus                     |       X        X  X  X  X                                               X|             
      Clear Cell Focus                     |                X     X  X  X                 X           X              X|             
      Congestion                           |                                                                          |             
      Degeneration, Cystic, Focal          |       1              1  1     2  2  2     1  2  1     1        2     2  1|             
      Eosinophilic Focus                   | X              X     X     X  X  X                       X           X   |             
      Fatty Change, Focal                  |    1                 2        1  3  2  1                    1           2|             
      Hepatodiaphragmatic Nodule           |                                                                          |             
      Inflammation, Chronic, Focal         |                                                                          |             
      Mixed Cell Focus                     |             X           X                                                |             
      Necrosis, Focal                      |                                                                          |             
      Pigmentation, Focal                  |                                  2                                       |             
      Bile Duct, Cyst                      |                                                                1         |             
      Bile Duct, Fibrosis                  |                                                                          |             
      Bile Duct, Hyperplasia, Focal        |                                                                          |             
      Centrilobular, Atrophy, Focal        |                                                                          |             
      Vein, Dilatation                     |                                           2                              |             
                                            __________________________________________________________________________|             
   Mesentery                               |          +                                                               |             
      Fat, Necrosis, Focal                 |                                                                          |             
                                            __________________________________________________________________________|             
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acinus, Atrophy, Diffuse             |                      4                                                   |             
      Acinus, Atrophy, Focal               | 2  2  2                    2     2        2  2           1  2  1         |             
      Acinus, Cytoplasmic Alteration, Focal|                                                                          |             
                                            __________________________________________________________________________|             
   Pharynx                                 |    +  +        +                             +                           |             
      Palate, Hyperplasia, Focal, Squamous |                3                                                         |             
                                            __________________________________________________________________________|             
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |                      1                                                   |             
                                            __________________________________________________________________________|             
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Acanthosis, Focal                    |                                                                          |             
      Hyperplasia, Basal Cell, Focal       |    1                                      1        1  2  2     1     1   |             
      Hyperplasia, Focal, Plasma Cell      |                                                                          |             
      Hyperplasia, Focal, Squamous         |                               2                                          |             
                                            __________________________________________________________________________|             
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Erosion, Focal                       |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM                     |                                                                          |             
                                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  96                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 5| 6| 7| 6| 7| 7| 7| 7| 6| 5| 5| 7| 7| 7| 6| 6| 7| 4| 7| 5| 7| 7|             
                             DAY ON TEST   | 2| 0| 2| 3| 0| 2| 1| 2| 2| 2| 2| 1| 3| 8| 0| 2| 2| 6| 9| 2| 5| 2| 0| 2| 2|             
                                           | 9| 2| 9| 2| 1| 9| 0| 9| 9| 9| 9| 4| 1| 0| 6| 9| 9| 8| 2| 9| 0| 2| 6| 9| 9|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|             
    10000PPM                               | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 CARDIOVASCULAR SYSTEM - cont              |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cardiomyopathy                       | 2     1        1  2  3  2  2  2     3        2  1  2           2     2   |             
      Mineralization, Focal                |                                                                2         |             
      Atrium, Thrombosis                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 ENDOCRINE SYSTEM                          |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                  3                                       |             
      Cyst, Multiple                       |                2                                                         |             
      Hemorrhage                           |                               2                                          |             
      Hyperplasia, Focal                   |                                                 1                        |             
      Vacuolization Cytoplasmic, Focal     |    2        2                    2           2                 1         |             
                                            __________________________________________________________________________|             
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                  3           1  1                        |             
      Bilateral, Hyperplasia               |                                           2                              |             
                                            __________________________________________________________________________|             
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia, Focal                   |       2              2              3     2                             3|             
                                            __________________________________________________________________________|             
   Parathyroid Gland                       | +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  M  +|             
      Bilateral, Hyperplasia               |                                                                4         |             
                                            __________________________________________________________________________|             
   Pituitary Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Pars Distalis, Hyperplasia, Focal    | 2  1                 2                       3           2     3        1|             
      Pars Nervosa, Hyperplasia, Focal     |                                                                2         |             
                                            __________________________________________________________________________|             
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      C-Cell, Hyperplasia, Focal           |                                              1        1  1               |             
      Follicle, Dilatation, Focal          |                               2  3                                       |             
      Follicular Cell, Hyperplasia, Focal  |                                                    2                     |             
 _____________________________________________________________________________________________________________________|             
 GENERAL BODY SYSTEM                       |                                                                          |             
                                           |                                                                          |             
    None                                   |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Degeneration, Focal                  |    2  1     2  3     2     2  1           3  2  2     2        3     2  1|             
                                            __________________________________________________________________________|             
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +|             
      Hyperplasia                          |                                                                          |             
      Inflammation, Chronic                |                                                                          |             
      Inflammation, Suppurative            |                                           3                              |             
      Duct, Dilatation                     |    3  2                 2                       2        3               |             
                                            __________________________________________________________________________|             
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Abscess                              |                                     3                          3         |             
      Atrophy                              |                            2  2                 2        2           2   |             
      Inflammation, Chronic, Focal         |                                                                         2|             
      Inflammation, Focal, Granulomatous   |                                  3                                       |             
      Inflammation, Focal, Suppurative     |                                              2           1               |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  97                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 5| 6| 7| 6| 7| 7| 7| 7| 6| 5| 5| 7| 7| 7| 6| 6| 7| 4| 7| 5| 7| 7|             
                             DAY ON TEST   | 2| 0| 2| 3| 0| 2| 1| 2| 2| 2| 2| 1| 3| 8| 0| 2| 2| 6| 9| 2| 5| 2| 0| 2| 2|             
                                           | 9| 2| 9| 2| 1| 9| 0| 9| 9| 9| 9| 4| 1| 0| 6| 9| 9| 8| 2| 9| 0| 2| 6| 9| 9|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|             
    10000PPM                               | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 GENITAL SYSTEM - cont                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Abscess                              |                                  3                                       |             
      Inflammation, Chronic, Focal         |                                                                          |             
                                            __________________________________________________________________________|             
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Atrophy                              |                            4     3     2  3           3                  |             
      Interstitial Cell, Hyperplasia, Focal|                                           2                              |             
 _____________________________________________________________________________________________________________________|             
 HEMATOPOIETIC SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hyperplasia                          |                2                 2                                       |             
                                            __________________________________________________________________________|             
   Lymph Node                              |    +  +                       +  +     +  +        +                 +   |             
      Axillary, Hyperplasia, Plasma Cell   |                                                                          |             
      Iliac, Cyst                          |                                                                          |             
      Iliac, Infiltration Cellular,        |                                                                          |             
          Histiocyte                       |                                                                          |             
      Inguinal, Cyst                       |                                                                          |             
      Lumbar, Hemorrhage                   |                                  3                                       |             
      Lumbar, Hyperplasia, Plasma Cell     |                                  3                                       |             
      Mediastinal, Hemorrhage              |                               2  3                                       |             
      Mediastinal, Infiltration Cellular,  |                                                                          |             
           Histiocyte                      |                                                                          |             
      Pancreatic, Hemorrhage               |                               3  3                                       |             
      Renal, Hemorrhage                    |                                                                          |             
      Renal, Infiltration Cellular,        |                                                                          |             
          Histiocyte                       |                                                                          |             
                                            __________________________________________________________________________|             
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                         3  3                                             |             
      Hemorrhage                           |                               2     3                                    |             
      Hyperplasia, Plasma Cell             |       2                                                                  |             
                                            __________________________________________________________________________|             
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Hemorrhage                           |                               3  2                                       |             
                                            __________________________________________________________________________|             
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  A  +  +|             
      Fibrosis, Focal                      |                2           2                                             |             
      Hematopoietic Cell Proliferation     |                                                                          |             
      Capsule, Cyst                        |                                                       2                  |             
                                            __________________________________________________________________________|             
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                     3                                    |             
      Hemorrhage                           |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM                      |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +|             
      Galactocele                          |                                                                          |             
      Hyperplasia, Focal                   |                                                                          |             
      Inflammation, Chronic, Focal         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  98                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 5| 6| 7| 6| 7| 7| 7| 7| 6| 5| 5| 7| 7| 7| 6| 6| 7| 4| 7| 5| 7| 7|             
                             DAY ON TEST   | 2| 0| 2| 3| 0| 2| 1| 2| 2| 2| 2| 1| 3| 8| 0| 2| 2| 6| 9| 2| 5| 2| 0| 2| 2|             
                                           | 9| 2| 9| 2| 1| 9| 0| 9| 9| 9| 9| 4| 1| 0| 6| 9| 9| 8| 2| 9| 0| 2| 6| 9| 9|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|             
    10000PPM                               | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 INTEGUMENTARY SYSTEM - cont               |                                                                          |             
                                           |                                                                          |             
      Pigmentation, Focal                  |                                  2                                       |             
                                            __________________________________________________________________________|             
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Subcutaneous Tissue, Inflammation,   |                                                                          |             
           Acute                           |                               2                                          |             
 _____________________________________________________________________________________________________________________|             
 MUSCULOSKELETAL SYSTEM                    |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Fibrous Osteodystrophy               |                                                                2         |             
      Hyperplasia, Focal                   |                                        3                                 |             
                                            __________________________________________________________________________|             
   Skeletal Muscle                         |                                                       +                  |             
 _____________________________________________________________________________________________________________________|             
 NERVOUS SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Gliosis, Focal                       |                                                                         2|             
      Hydrocephalus                        |                   2                                                      |             
 _____________________________________________________________________________________________________________________|             
 RESPIRATORY SYSTEM                        |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Congestion                           |                                     3                                    |             
      Hemorrhage, Focal                    |                                                                          |             
      Infiltration Cellular, Focal,        |                                                                          |             
          Histiocyte                       |                                                                          |             
      Inflammation, Focal                  |                      1        3  2                             3         |             
      Alveolar Epithelium, Hyperplasia,    |                                                                          |             
           Focal                           |          3                                                               |             
                                            __________________________________________________________________________|             
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Foreign Body                         |       3  2                                                               |             
      Fungus                               |       3  3  3     2     3  3     3                                       |             
      Hemorrhage                           |                                                                          |             
      Inflammation, Chronic Active         |       3  4  3     2     3  3     3                                       |             
      Inflammation, Subacute               |                                                                1  1      |             
      Glands, Dilatation, Focal            |    2                                                                     |             
                                            __________________________________________________________________________|             
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
 _____________________________________________________________________________________________________________________|             
 SPECIAL SENSES SYSTEM                     |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Eye                                     |                                                       +                  |             
      Lens, Cataract                       |                                                                          |             
      Retina, Atrophy                      |                                                                          |             
                                            __________________________________________________________________________|             
   Zymbal's Gland                          | M  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  M  +  +  +  +  +  +|             
      Inflammation, Chronic Active         |                                                                          |             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM                            |                                                                          |             
                                           |                                                                          |             
                                            __________________________________________________________________________|             
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Cyst                                 |                                                       2                  |             
 _____________________________________________________________________________________________________________________|             
                                                             Page  99                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 _____________________________________________________________________________________________________________________              
                                           | 7| 7| 7| 5| 6| 7| 6| 7| 7| 7| 7| 6| 5| 5| 7| 7| 7| 6| 6| 7| 4| 7| 5| 7| 7|             
                             DAY ON TEST   | 2| 0| 2| 3| 0| 2| 1| 2| 2| 2| 2| 1| 3| 8| 0| 2| 2| 6| 9| 2| 5| 2| 0| 2| 2|             
                                           | 9| 2| 9| 2| 1| 9| 0| 9| 9| 9| 9| 4| 1| 0| 6| 9| 9| 8| 2| 9| 0| 2| 6| 9| 9|             
 _____________________________________________________________________________________________________________________|             
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|             
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|             
    10000PPM                               | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|             
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|             
 _____________________________________________________________________________________________________________________|             
 URINARY SYSTEM - cont                     |                                                                          |             
                                           |                                                                          |             
      Infarct                              |    4                                                                     |             
      Mineralization, Focal                |                                                                          |             
      Nephropathy                          | 3  2  4     2  3  3  3  3  3  2  2  1  2  2  3  3  2  3  3  1  4     2  2|             
      Pigmentation, Focal                  |    3  1  2  3  1  2              1  2  2  2           2              2   |             
      Pelvis, Inflammation, Suppurative    |                                                                          |             
                                            __________________________________________________________________________|             
   Urethra                                 |                                                                          |             
                                            __________________________________________________________________________|             
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +  +|             
      Abscess                              |                                                                          |             
 _____________________________________________________________________________________________________________________|             
                                                             Page 100                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 7|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |      A     |
    10000PPM                               | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Esophagus                               |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Colon                  |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Rectum                 |                                                                          |  50        |
                                            __________________________________________________________________________|____________|
   Intestine Large, Cecum                  |                                                                          |  49        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Duodenum               |                                                                          |  48        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Jejunum                |                                                                          |  46        |
                                            __________________________________________________________________________|____________|
   Intestine Small, Ileum                  |                                                                          |  47        |
                                            __________________________________________________________________________|____________|
   Liver                                   |                                                                          |  50        |
      Angiectasis, Focal                   |                                                                          |      2  2.0|
      Basophilic Focus                     |                                                                          |     18     |
      Clear Cell Focus                     |                                                                          |     17     |
      Congestion                           |                                                                          |      1  2.0|
      Degeneration, Cystic, Focal          |                                                                          |     25  1.6|
      Eosinophilic Focus                   |                                                                          |     20     |
      Fatty Change, Focal                  |                                                                          |     16  1.5|
      Hepatodiaphragmatic Nodule           |                                                                          |      1     |
      Inflammation, Chronic, Focal         |                                                                          |      2  1.5|
      Mixed Cell Focus                     |                                                                          |      7     |
      Necrosis, Focal                      |                                                                          |      3  2.3|
      Pigmentation, Focal                  |                                                                          |      1  2.0|
      Bile Duct, Cyst                      |                                                                          |      1  1.0|
      Bile Duct, Fibrosis                  |                                                                          |      1  4.0|
      Bile Duct, Hyperplasia, Focal        |                                                                          |      2  3.0|
      Centrilobular, Atrophy, Focal        |                                                                          |      1  2.0|
      Vein, Dilatation                     |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Mesentery                               |                                                                          |   4        |
      Fat, Necrosis, Focal                 |                                                                          |      3  3.0|
                                            __________________________________________________________________________|____________|
   Pancreas                                |                                                                          |  50        |
      Acinus, Atrophy, Diffuse             |                                                                          |      1  4.0|
      Acinus, Atrophy, Focal               |                                                                          |     26  1.7|
      Acinus, Cytoplasmic Alteration, Focal|                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Pharynx                                 |                                                                          |   9        |
      Palate, Hyperplasia, Focal, Squamous |                                                                          |      2  3.5|
                                            __________________________________________________________________________|____________|
   Salivary Glands                         |                                                                          |  50        |
      Hyperplasia, Focal                   |                                                                          |      1  1.0|
                                            __________________________________________________________________________|____________|
   Stomach, Forestomach                    |                                                                          |  50        |
      Acanthosis, Focal                    |                                                                          |      1  1.0|
      Hyperplasia, Basal Cell, Focal       |                                                                          |     19  1.4|
      Hyperplasia, Focal, Plasma Cell      |                                                                          |      1  2.0|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page 101                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 7|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |      A     |
    10000PPM                               | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 ALIMENTARY SYSTEM - cont                  |                                                                          |            |
                                           |                                                                          |            |
      Hyperplasia, Focal, Squamous         |                                                                          |      4  1.5|
                                            __________________________________________________________________________|____________|
   Stomach, Glandular                      |                                                                          |  50        |
      Erosion, Focal                       |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Heart                                   |                                                                          |  50        |
      Cardiomyopathy                       |                                                                          |     32  1.9|
      Mineralization, Focal                |                                                                          |      1  2.0|
      Atrium, Thrombosis                   |                                                                          |      1  3.0|
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Adrenal Cortex                          |                                                                          |  50        |
      Congestion                           |                                                                          |      1  3.0|
      Cyst, Multiple                       |                                                                          |      1  2.0|
      Hemorrhage                           |                                                                          |      1  2.0|
      Hyperplasia, Focal                   |                                                                          |      2  1.5|
      Vacuolization Cytoplasmic, Focal     |                                                                          |     12  2.1|
                                            __________________________________________________________________________|____________|
   Adrenal Medulla                         |                                                                          |  50        |
      Hyperplasia                          |                                                                          |      8  1.9|
      Bilateral, Hyperplasia               |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Islets, Pancreatic                      |                                                                          |  50        |
      Hyperplasia, Focal                   |                                                                          |     10  2.6|
                                            __________________________________________________________________________|____________|
   Parathyroid Gland                       |                                                                          |  45        |
      Bilateral, Hyperplasia               |                                                                          |      2  3.5|
                                            __________________________________________________________________________|____________|
   Pituitary Gland                         |                                                                          |  50        |
      Pars Distalis, Hyperplasia, Focal    |                                                                          |     18  1.8|
      Pars Nervosa, Hyperplasia, Focal     |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Thyroid Gland                           |                                                                          |  50        |
      C-Cell, Hyperplasia, Focal           |                                                                          |      4  1.0|
      Follicle, Dilatation, Focal          |                                                                          |      4  2.5|
      Follicular Cell, Hyperplasia, Focal  |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Epididymis                              |                                                                          |  50        |
      Degeneration, Focal                  |                                                                          |     33  2.0|
                                            __________________________________________________________________________|____________|
   Preputial Gland                         |                                                                          |  49        |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page 102                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 7|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |      A     |
    10000PPM                               | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 GENITAL SYSTEM - cont                     |                                                                          |            |
                                           |                                                                          |            |
      Hyperplasia                          |                                                                          |      1  3.0|
      Inflammation, Chronic                |                                                                          |      1  2.0|
      Inflammation, Suppurative            |                                                                          |      1  3.0|
      Duct, Dilatation                     |                                                                          |     11  2.4|
                                            __________________________________________________________________________|____________|
   Prostate                                |                                                                          |  49        |
      Abscess                              |                                                                          |      2  3.0|
      Atrophy                              |                                                                          |     13  2.1|
      Inflammation, Chronic, Focal         |                                                                          |      4  2.3|
      Inflammation, Focal, Granulomatous   |                                                                          |      2  2.5|
      Inflammation, Focal, Suppurative     |                                                                          |      4  2.3|
                                            __________________________________________________________________________|____________|
   Seminal Vesicle                         |                                                                          |  49        |
      Abscess                              |                                                                          |      1  3.0|
      Inflammation, Chronic, Focal         |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Testes                                  |                                                                          |  50        |
      Atrophy                              |                                                                          |      9  3.2|
      Interstitial Cell, Hyperplasia, Focal|                                                                          |      5  1.8|
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Bone Marrow                             |                                                                          |  50        |
      Hyperplasia                          |                                                                          |      4  2.5|
                                            __________________________________________________________________________|____________|
   Lymph Node                              |                                                                          |  18        |
      Axillary, Hyperplasia, Plasma Cell   |                                                                          |      1  2.0|
      Iliac, Cyst                          |                                                                          |      1     |
      Iliac, Infiltration Cellular,        |                                                                          |            |
          Histiocyte                       |                                                                          |      1  2.0|
      Inguinal, Cyst                       |                                                                          |      1     |
      Lumbar, Hemorrhage                   |                                                                          |      2  2.5|
      Lumbar, Hyperplasia, Plasma Cell     |                                                                          |      1  3.0|
      Mediastinal, Hemorrhage              |                                                                          |      2  2.5|
      Mediastinal, Infiltration Cellular,  |                                                                          |            |
           Histiocyte                      |                                                                          |      1  3.0|
      Pancreatic, Hemorrhage               |                                                                          |      2  3.0|
      Renal, Hemorrhage                    |                                                                          |      1  2.0|
      Renal, Infiltration Cellular,        |                                                                          |            |
          Histiocyte                       |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Lymph Node, Mandibular                  |                                                                          |  50        |
      Cyst                                 |                                                                          |      3  3.0|
      Hemorrhage                           |                                                                          |      2  2.5|
      Hyperplasia, Plasma Cell             |                                                                          |      3  2.0|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page 103                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 7|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |      A     |
    10000PPM                               | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  |                                                                          |  50        |
      Hemorrhage                           |                                                                          |      3  2.0|
                                            __________________________________________________________________________|____________|
   Spleen                                  |                                                                          |  49        |
      Fibrosis, Focal                      |                                                                          |      4  2.0|
      Hematopoietic Cell Proliferation     |                                                                          |      2  2.5|
      Capsule, Cyst                        |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Thymus                                  |                                                                          |  48        |
      Congestion                           |                                                                          |      1  3.0|
      Hemorrhage                           |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Mammary Gland                           |                                                                          |  46        |
      Galactocele                          |                                                                          |      1  3.0|
      Hyperplasia, Focal                   |                                                                          |      2  2.5|
      Inflammation, Chronic, Focal         |                                                                          |      1  3.0|
      Pigmentation, Focal                  |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Skin                                    |                                                                          |  50        |
      Subcutaneous Tissue, Inflammation,   |                                                                          |            |
           Acute                           |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Bone                                    |                                                                          |  50        |
      Fibrous Osteodystrophy               |                                                                          |      1  2.0|
      Hyperplasia, Focal                   |                                                                          |      1  3.0|
                                            __________________________________________________________________________|____________|
   Skeletal Muscle                         |                                                                          |   1        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Brain                                   |                                                                          |  50        |
      Gliosis, Focal                       |                                                                          |      1  2.0|
      Hydrocephalus                        |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Lung                                    |                                                                          |  50        |
      Congestion                           |                                                                          |      3  2.7|
      Hemorrhage, Focal                    |                                                                          |      1  2.0|
      Infiltration Cellular, Focal,        |                                                                          |            |
          Histiocyte                       |                                                                          |      4  1.3|
      Inflammation, Focal                  |                                                                          |      7  1.9|
      Alveolar Epithelium, Hyperplasia,    |                                                                          |            |
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page 104                                                               
NTP Experiment-Test: 05130-03                  NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL                         Report: PEIRPT09
Study Type: CHRONIC                                       C. I. DIRECT BLUE 218                                   Date: 04/24/97  
Route: DOSED FEED                                                                                                 Time: 14:07:43  
                                                                                                                                    
 __________________________________________________________________________________________________________________________________ 
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |            |
                             DAY ON TEST   | 6| 6| 6| 6| 6| 6| 6| 6| 6| 4|                                            |            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 7|                                            |      T (*) |
 _____________________________________________________________________________________________________________________|            |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      O     |
   FISCHER 344 RATS MALE                   | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                                            |      T     |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                                            |      A     |
    10000PPM                               | 7| 7| 7| 7| 7| 8| 8| 8| 8| 8|                                            |      L     |
                                           | 1| 2| 3| 4| 5| 1| 2| 3| 4| 5|                                            |            |
 __________________________________________________________________________________________________________________________________ 
 RESPIRATORY SYSTEM - cont                 |                                                                          |            |
                                           |                                                                          |            |
           Focal                           |                                                                          |      2  2.5|
                                            __________________________________________________________________________|____________|
   Nose                                    |                                                                          |  50        |
      Foreign Body                         |                                                                          |      3  2.3|
      Fungus                               |                                                                          |     11  2.7|
      Hemorrhage                           |                                                                          |      1  2.0|
      Inflammation, Chronic Active         |                                                                          |     14  2.7|
      Inflammation, Subacute               |                                                                          |      3  1.3|
      Glands, Dilatation, Focal            |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Trachea                                 |                                                                          |  50        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Eye                                     |                                                                          |   2        |
      Lens, Cataract                       |                                                                          |      1  2.0|
      Retina, Atrophy                      |                                                                          |      1  4.0|
                                            __________________________________________________________________________|____________|
   Zymbal's Gland                          |                                                                          |  48        |
      Inflammation, Chronic Active         |                                                                          |      1  2.0|
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Kidney                                  |                                                                          |  50        |
      Cyst                                 |                                                                          |      2  2.5|
      Infarct                              |                                                                          |      1  4.0|
      Mineralization, Focal                |                                                                          |      1  1.0|
      Nephropathy                          |                                                                          |     46  2.6|
      Pigmentation, Focal                  |                                                                          |     23  2.0|
      Pelvis, Inflammation, Suppurative    |                                                                          |      1  2.0|
                                            __________________________________________________________________________|____________|
   Urethra                                 |                                                                          |   1        |
                                            __________________________________________________________________________|____________|
   Urinary Bladder                         |                                                                          |  50        |
      Abscess                              |                                                                          |      1  3.0|
 __________________________________________________________________________________________________________________________________ 
  * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade                         
  + : Tissue examined microscopically                M : Missing tissue                        1-4 : Lesion qualified as:           
  X : Lesion present but not qualified               A : Autolysis precludes examination               1) Minimal  3) Moderate      
  I : Insufficient tissue                        BLANK : Not examined                                  2) Mild     4) Marked        
                                                             Page 105                                                               
                             ------------------------------------------------------------                                           
                             ----------              END OF REPORT             ----------                                           
                             ------------------------------------------------------------