National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/2924

TDMS Study 05133-03 Pathology Tables


                                     INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)
                                                            ACETONITRILE


NTP Experiment-Test: 05133-03                                                                                     Report: PEIRPT03
Study Type: CHRONIC                                                                                               Date: 05/20/95
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 06:11:11




       Facility:  Battelle Northwest

       Chemical CAS #:  75-05-8

       Lock Date:  05/15/92

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All
































a  Number of animals examined microscopically at site and number of animals with lesion

                                                             Page   1

NTP Experiment-Test: 05133-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                            ACETONITRILE                                       Date: 05/20/95  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 06:11:11  
____________________________________________________________________________________________________________________________________
         FISCHER 344 RATS FEMALE                   CONTROL      100 PPM      200 PPM      400 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY                                                                                                                 
  Animals Initially In Study                          56           56           56           56                                     
  Scheduled Sacrifice                                  8            8            8            8                                     
  Early Deaths                                                                                                                      
    Moribund Sacrifice                                24           25           21           14                                     
    Accidently Killed                                  1                                                                            
    Natural Death                                                   2            1            5                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                23           21           26           29                                     
  Animals Examined Microscopically                    56           56           56           56                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Esophagus                                          (56)         (56)         (56)         (55)                                   
      Mediastinum, Cyst                                                          1 (2%)                                             
   Intestine Large, Colon                             (55)         (55)         (56)         (53)                                   
      Inflammation, Suppurative                                     1 (2%)                                                          
      Lumen, Parasite Metazoan                         2 (4%)                                 1 (2%)                                
   Intestine Large, Rectum                            (55)         (56)         (56)         (53)                                   
      Ulcer                                                         1 (2%)                                                          
      Lumen, Parasite Metazoan                         2 (4%)                    6 (11%)      3 (6%)                                
   Intestine Large, Cecum                             (55)         (54)         (55)         (53)                                   
      Lumen, Parasite Metazoan                                      1 (2%)       2 (4%)                                             
   Intestine Small, Jejunum                           (55)         (55)         (56)         (53)                                   
      Inflammation, Granulomatous                                   1 (2%)                                                          
   Intestine Small, Ileum                             (55)         (55)         (54)         (53)                                   
      Inflammation, Granulomatous                                   1 (2%)                                                          
   Liver                                              (56)         (56)         (56)         (54)                                   
      Angiectasis                                                                             1 (2%)                                
      Atrophy                                                       1 (2%)                                                          
      Basophilic Focus                                40 (71%)     41 (73%)     38 (68%)     42 (78%)                               
      Clear Cell Focus                                 2 (4%)       2 (4%)       1 (2%)                                             
      Degeneration, Cystic                             1 (2%)                                 1 (2%)                                
      Eosinophilic Focus                                                         1 (2%)                                             
      Granuloma, Multifocal                                         4 (7%)       2 (4%)       3 (6%)                                
      Hematopoietic Cell Proliferation                 1 (2%)       1 (2%)                                                          
      Hepatodiaphragmatic Nodule                       7 (13%)      3 (5%)       8 (14%)      8 (15%)                               
      Infarct                                          1 (2%)                                                                       
      Mixed Cell Focus                                                                        2 (4%)                                
      Vacuolization Cytoplasmic                       11 (20%)     14 (25%)     12 (21%)     20 (37%)                               
      Bile Duct, Hyperplasia                           2 (4%)                                                                       
      Hepatocyte, Degeneration, Cystic                              1 (2%)       1 (2%)       3 (6%)                                
      Hepatocyte, Hyperplasia                          7 (13%)      9 (16%)      4 (7%)      10 (19%)                               
      Hepatocyte, Necrosis                             1 (2%)                    3 (5%)                                             
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    2                                                              
NTP Experiment-Test: 05133-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                            ACETONITRILE                                       Date: 05/20/95  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 06:11:11  
____________________________________________________________________________________________________________________________________
         FISCHER 344 RATS FEMALE                   CONTROL      100 PPM      200 PPM      400 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
      Portal, Fibrosis                                 1 (2%)                                                                       
      Serosa, Cyst                                                  1 (2%)                                                          
      Serosa, Hemorrhage                                            1 (2%)       1 (2%)                                             
   Mesentery                                          (10)         (9)          (8)          (11)                                   
      Hemorrhage                                       1 (10%)      1 (11%)      2 (25%)      1 (9%)                                
      Artery, Inflammation                                          1 (11%)      1 (13%)                                            
      Fat, Inflammation, Granulomatous                 1 (10%)      6 (67%)      4 (50%)      7 (64%)                               
      Fat, Necrosis                                    7 (70%)      2 (22%)      4 (50%)      9 (82%)                               
   Pancreas                                           (56)         (56)         (56)         (54)                                   
      Acinus, Atrophy                                 15 (27%)     22 (39%)     29 (52%)     27 (50%)                               
      Artery, Inflammation                                                       1 (2%)       1 (2%)                                
   Salivary Glands                                    (56)         (54)         (56)         (55)                                   
      Duct, Mineralization                             1 (2%)                                                                       
   Stomach, Forestomach                               (56)         (56)         (55)         (54)                                   
      Diverticulum                                                               2 (4%)                                             
      Edema                                            2 (4%)       3 (5%)       5 (9%)                                             
      Hyperplasia, Basal Cell                                                    1 (2%)       2 (4%)                                
      Hyperplasia, Squamous                            3 (5%)       6 (11%)      8 (15%)      7 (13%)                               
      Inflammation, Suppurative                        2 (4%)       5 (9%)       4 (7%)       3 (6%)                                
      Necrosis                                                                   1 (2%)                                             
      Ulcer                                            2 (4%)       3 (5%)       5 (9%)       5 (9%)                                
   Stomach, Glandular                                 (55)         (56)         (56)         (54)                                   
      Mineralization                                                                          1 (2%)                                
      Necrosis                                         1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Blood Vessel                                       (56)         (56)         (56)         (55)                                   
      Aorta, Inflammation                                                        1 (2%)                                             
      Aorta, Mineralization                                                                   1 (2%)                                
   Heart                                              (56)         (56)         (56)         (55)                                   
      Cardiomyopathy                                   2 (4%)       2 (4%)       2 (4%)       2 (4%)                                
      Thrombosis                                       1 (2%)       1 (2%)                    1 (2%)                                
      Endocardium, Hyperplasia                         1 (2%)                                 1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (56)         (56)         (56)         (54)                                   
      Hemorrhage                                       1 (2%)                    1 (2%)                                             
      Hyperplasia                                      1 (2%)       2 (4%)                                                          
      Mineralization                                                                          1 (2%)                                
      Vacuolization Cytoplasmic                        6 (11%)     11 (20%)      5 (9%)       8 (15%)                               
   Adrenal Medulla                                    (56)         (55)         (55)         (55)                                   
      Angiectasis                                                                1 (2%)                                             
      Hyperplasia                                      5 (9%)       6 (11%)      3 (5%)       1 (2%)                                
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    3                                                              
NTP Experiment-Test: 05133-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                            ACETONITRILE                                       Date: 05/20/95  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 06:11:11  
____________________________________________________________________________________________________________________________________
         FISCHER 344 RATS FEMALE                   CONTROL      100 PPM      200 PPM      400 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ENDOCRINE SYSTEM - CONT                                                                                                             
   Parathyroid Gland                                  (52)         (52)         (51)         (52)                                   
      Hyperplasia                                                                3 (6%)       1 (2%)                                
   Pituitary Gland                                    (56)         (56)         (55)         (53)                                   
      Cyst                                            13 (23%)     17 (30%)     14 (25%)     15 (28%)                               
      Pars Distalis, Cyst                              1 (2%)       1 (2%)                    1 (2%)                                
      Pars Distalis, Hemorrhage                                     1 (2%)                                                          
      Pars Distalis, Hyperplasia                       9 (16%)      5 (9%)       3 (5%)       3 (6%)                                
   Thyroid Gland                                      (56)         (56)         (56)         (54)                                   
      Dilatation                                       1 (2%)                                                                       
      Ultimobranchial Cyst                                                       1 (2%)                                             
      C-Cell, Hyperplasia                             26 (46%)     39 (70%)     36 (64%)     39 (72%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Clitoral Gland                                     (54)         (50)         (55)         (53)                                   
      Ectasia                                         11 (20%)      4 (8%)      10 (18%)     14 (26%)                               
      Hyperplasia                                      2 (4%)       4 (8%)       4 (7%)       3 (6%)                                
      Inflammation, Suppurative                        4 (7%)       3 (6%)       2 (4%)       6 (11%)                               
   Ovary                                              (56)         (56)         (56)         (55)                                   
      Cyst                                             4 (7%)       3 (5%)       2 (4%)       5 (9%)                                
      Inflammation, Granulomatous                                   1 (2%)                                                          
   Uterus                                             (56)         (56)         (56)         (55)                                   
      Hemorrhage                                       1 (2%)                                                                       
      Thrombosis                                       1 (2%)                                                                       
      Cervix, Endometrium, Hyperplasia                              1 (2%)       1 (2%)                                             
      Endometrium, Hyperplasia                         1 (2%)                    2 (4%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (56)         (56)         (56)         (54)                                   
      Hyperplasia, Re Cell                             1 (2%)       3 (5%)       2 (4%)       1 (2%)                                
      Myelofibrosis                                                 2 (4%)                                                          
   Lymph Node                                         (7)          (6)          (9)          (8)                                    
      Axillary, Hyperplasia, Lymphoid                                            1 (11%)                                            
      Iliac, Pigmentation                                                        1 (11%)                                            
      Lumbar, Pigmentation                             1 (14%)                                                                      
      Pancreatic, Hemorrhage                                        1 (17%)                                                         
      Pancreatic, Pigmentation                                                   1 (11%)                                            
      Renal, Hemorrhage                                             1 (17%)      1 (11%)                                            
   Lymph Node, Bronchial                              (49)         (44)         (45)         (48)                                   
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    4                                                              
NTP Experiment-Test: 05133-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                            ACETONITRILE                                       Date: 05/20/95  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 06:11:11  
____________________________________________________________________________________________________________________________________
         FISCHER 344 RATS FEMALE                   CONTROL      100 PPM      200 PPM      400 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - CONT                                                                                                         
      Hemorrhage                                       4 (8%)       1 (2%)                    2 (4%)                                
      Inflammation, Suppurative                        1 (2%)                                                                       
   Lymph Node, Mandibular                             (54)         (54)         (52)         (49)                                   
      Hemorrhage                                       1 (2%)                                                                       
      Hyperplasia, Lymphoid                            2 (4%)       1 (2%)       4 (8%)                                             
   Lymph Node, Mesenteric                             (54)         (55)         (55)         (55)                                   
      Hemorrhage                                                    3 (5%)                    2 (4%)                                
      Hyperplasia, Lymphoid                            2 (4%)       1 (2%)                                                          
      Inflammation, Granulomatous                                   1 (2%)                                                          
   Lymph Node, Mediastinal                            (56)         (53)         (53)         (54)                                   
      Hemorrhage                                      11 (20%)      7 (13%)      3 (6%)      11 (20%)                               
      Hyperplasia, Lymphoid                                                      1 (2%)                                             
      Pigmentation                                                                            1 (2%)                                
   Spleen                                             (56)         (56)         (56)         (53)                                   
      Developmental Malformation                                                              1 (2%)                                
      Fibrosis                                         1 (2%)       2 (4%)       1 (2%)       3 (6%)                                
      Hematopoietic Cell Proliferation                                                        1 (2%)                                
      Hemorrhage                                       1 (2%)                    2 (4%)                                             
      Hyperplasia, Re Cell                             2 (4%)       3 (5%)       1 (2%)                                             
      Necrosis                                         1 (2%)       1 (2%)       1 (2%)       1 (2%)                                
   Thymus                                             (56)         (56)         (55)         (55)                                   
      Hemorrhage                                                    1 (2%)                                                          
      Hyperplasia, Lymphoid                                                      1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (56)         (56)         (56)         (55)                                   
      Galactocele                                                   2 (4%)       2 (4%)                                             
      Epithelium, Hyperplasia                                                    1 (2%)                                             
   Skin                                               (56)         (56)         (56)         (56)                                   
      Acanthosis                                                    1 (2%)       1 (2%)                                             
      Inflammation, Granulomatous                                                2 (4%)       1 (2%)                                
      Inflammation, Suppurative                        1 (2%)       2 (4%)                                                          
      Ulcer                                                         2 (4%)       1 (2%)       2 (4%)                                
      Subcutaneous Tissue, Hemorrhage                                            1 (2%)                                             
      Subcutaneous Tissue, Necrosis                                                           1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (55)         (56)         (56)         (55)                                   
      Fibrosis                                                                                1 (2%)                                
      Periosteum, Cranium, Hemorrhage                                                         1 (2%)                                
      Vertebra, Fracture                               1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    5                                                              
NTP Experiment-Test: 05133-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                            ACETONITRILE                                       Date: 05/20/95  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 06:11:11  
____________________________________________________________________________________________________________________________________
         FISCHER 344 RATS FEMALE                   CONTROL      100 PPM      200 PPM      400 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (56)         (56)         (56)         (55)                                   
      Hemorrhage                                       4 (7%)       5 (9%)       8 (14%)      2 (4%)                                
      Hydrocephalus                                    4 (7%)       6 (11%)      3 (5%)       5 (9%)                                
      Necrosis                                         1 (2%)                                                                       
   Spinal Cord                                        (1)                                                                           
      Hemorrhage                                       1 (100%)                                                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Larynx                                             (55)         (56)         (56)         (54)                                   
      Foreign Body                                     8 (15%)      2 (4%)       4 (7%)       4 (7%)                                
      Hyperplasia                                      5 (9%)       1 (2%)       3 (5%)       4 (7%)                                
      Inflammation, Suppurative                        5 (9%)                    6 (11%)      3 (6%)                                
      Metaplasia, Squamous                             7 (13%)      5 (9%)       7 (13%)      3 (6%)                                
      Mineralization                                                             1 (2%)                                             
   Lung                                               (56)         (56)         (56)         (54)                                   
      Hemorrhage                                      43 (77%)     37 (66%)     40 (71%)     38 (70%)                               
      Infiltration Cellular, Histiocyte               11 (20%)     17 (30%)     17 (30%)     13 (24%)                               
      Metaplasia, Osseous                                                        1 (2%)                                             
      Alveolar Epithelium, Hyperplasia                 6 (11%)      5 (9%)       2 (4%)       1 (2%)                                
      Alveolus, Fibrosis                               2 (4%)       1 (2%)       1 (2%)                                             
      Alveolus, Inflammation, Chronic                  3 (5%)       1 (2%)                    1 (2%)                                
      Alveolus, Inflammation, Suppurative              2 (4%)                                                                       
      Pleura, Fibrosis                                 3 (5%)       2 (4%)       2 (4%)       1 (2%)                                
   Nose                                               (55)         (55)         (56)         (54)                                   
      Foreign Body                                     2 (4%)       5 (9%)       1 (2%)       3 (6%)                                
      Hemorrhage                                                    1 (2%)       1 (2%)       1 (2%)                                
      Inflammation, Chronic                            1 (2%)                    1 (2%)       2 (4%)                                
      Inflammation, Suppurative                        1 (2%)       2 (4%)       3 (5%)       2 (4%)                                
      Goblet Cell, Respiratory Epithelium,                                                                                          
          Hypertrophy                                  3 (5%)       2 (4%)       2 (4%)       4 (7%)                                
      Nasolacrimal Duct, Inflammation, Suppurative                  3 (5%)                    2 (4%)                                
      Olfactory Epithelium, Metaplasia                                                        1 (2%)                                
      Respiratory Epithelium, Hyperplasia              2 (4%)       2 (4%)       2 (4%)       1 (2%)                                
      Respiratory Epithelium, Metaplasia, Squamous                               1 (2%)       1 (2%)                                
   Trachea                                            (56)         (56)         (56)         (53)                                   
      Epithelium, Hyperplasia                                                                 1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                (4)          (2)          (4)          (4)                                    
      Cataract                                         1 (25%)                   2 (50%)      1 (25%)                               
      Hemorrhage                                       2 (50%)      1 (50%)      2 (50%)      1 (25%)                               
      Retina, Atrophy                                                            1 (25%)                                            
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    6                                                              
NTP Experiment-Test: 05133-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                            ACETONITRILE                                       Date: 05/20/95  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 06:11:11  
____________________________________________________________________________________________________________________________________
         FISCHER 344 RATS FEMALE                   CONTROL      100 PPM      200 PPM      400 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (56)         (56)         (56)         (54)                                   
      Infarct                                                       1 (2%)                                                          
      Nephropathy, Chronic                            40 (71%)     38 (68%)     38 (68%)     40 (74%)                               
      Cortex, Necrosis                                 5 (9%)       2 (4%)       5 (9%)       5 (9%)                                
      Pelvis, Transitional Epithelium, Hyperplasia                  1 (2%)                                                          
      Renal Tubule, Inflammation, Suppurative                       1 (2%)                                                          
      Renal Tubule, Mineralization                    44 (79%)     42 (75%)     48 (86%)     41 (76%)                               
      Renal Tubule, Pigmentation, Hemosiderin                       1 (2%)                                                          
   Urinary Bladder                                    (56)         (56)         (55)         (53)                                   
      Transitional Epithelium, Hyperplasia                          1 (2%)                                                          
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    7                                                              
NTP Experiment-Test: 05133-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                            ACETONITRILE                                       Date: 05/20/95  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 06:11:11  
____________________________________________________________________________________________________________________________________
         FISCHER 344 RATS MALE                     CONTROL      100 PPM      200 PPM      400 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY                                                                                                                 
  Animals Initially In Study                          56           56           56           56                                     
  Scheduled Sacrifice                                  8            8            8            8                                     
  Early Deaths                                                                                                                      
    Moribund Sacrifice                                36           26           35           26                                     
    Natural Death                                      1            8            4            5                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                11           13            9           17                                     
  Other                                                             1                                                               
  Animals Examined Microscopically                    56           56           56           56                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Intestine Large, Colon                             (56)         (50)         (54)         (53)                                   
      Lumen, Parasite Metazoan                         4 (7%)       2 (4%)       4 (7%)       1 (2%)                                
   Intestine Large, Rectum                            (55)         (52)         (55)         (54)                                   
      Lumen, Parasite Metazoan                         4 (7%)       4 (8%)       6 (11%)      5 (9%)                                
   Intestine Large, Cecum                             (55)         (51)         (54)         (52)                                   
      Lumen, Parasite Metazoan                         4 (7%)       3 (6%)       1 (2%)                                             
   Intestine Small, Duodenum                          (55)         (53)         (56)         (55)                                   
      Diverticulum                                                  1 (2%)                                                          
   Intestine Small, Jejunum                           (55)         (50)         (54)         (52)                                   
      Parasite Metazoan                                1 (2%)                                 1 (2%)                                
   Liver                                              (56)         (55)         (56)         (56)                                   
      Angiectasis                                                   1 (2%)       1 (2%)                                             
      Basophilic Focus                                21 (38%)     28 (51%)     32 (57%)     38 (68%)                               
      Clear Cell Focus                                 6 (11%)      1 (2%)       2 (4%)       7 (13%)                               
      Degeneration, Cystic                            16 (29%)     17 (31%)      8 (14%)      4 (7%)                                
      Eosinophilic Focus                               3 (5%)       7 (13%)      5 (9%)      10 (18%)                               
      Granuloma, Multifocal                                         1 (2%)                                                          
      Hematopoietic Cell Proliferation                 2 (4%)                                                                       
      Hepatodiaphragmatic Nodule                       2 (4%)       4 (7%)       5 (9%)       3 (5%)                                
      Inflammation, Chronic                            1 (2%)                                                                       
      Mixed Cell Focus                                 1 (2%)       1 (2%)       1 (2%)       5 (9%)                                
      Pigmentation, Hemosiderin                                     1 (2%)                                                          
      Thrombosis                                                                              2 (4%)                                
      Vacuolization Cytoplasmic                       11 (20%)     10 (18%)     12 (21%)      5 (9%)                                
      Bile Duct, Hyperplasia                           2 (4%)       2 (4%)       3 (5%)                                             
      Hepatocyte, Necrosis                             7 (13%)      8 (15%)      1 (2%)       3 (5%)                                
      Portal, Fibrosis                                 1 (2%)                                                                       
   Mesentery                                          (10)         (9)          (9)          (12)                                   
      Hemorrhage                                       3 (30%)                   1 (11%)      4 (33%)                               
      Fat, Inflammation, Granulomatous                 1 (10%)      1 (11%)      3 (33%)      2 (17%)                               
      Fat, Necrosis                                    7 (70%)      7 (78%)      1 (11%)      8 (67%)                               
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    8                                                              
NTP Experiment-Test: 05133-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                            ACETONITRILE                                       Date: 05/20/95  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 06:11:11  
____________________________________________________________________________________________________________________________________
         FISCHER 344 RATS MALE                     CONTROL      100 PPM      200 PPM      400 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
   Pancreas                                           (56)         (55)         (56)         (56)                                   
      Hemorrhage                                                                              2 (4%)                                
      Acinus, Atrophy                                 24 (43%)     35 (64%)     29 (52%)     31 (55%)                               
      Artery, Inflammation                                          1 (2%)                    2 (4%)                                
      Duct, Cyst                                       1 (2%)       1 (2%)                                                          
      Duct, Hyperplasia                                             1 (2%)                                                          
   Stomach, Forestomach                               (56)         (55)         (56)         (55)                                   
      Acanthosis                                       4 (7%)       4 (7%)      10 (18%)      4 (7%)                                
      Diverticulum                                                               2 (4%)       2 (4%)                                
      Edema                                            1 (2%)       3 (5%)       1 (2%)                                             
      Hyperkeratosis                                                2 (4%)                                                          
      Inflammation, Suppurative                        5 (9%)       4 (7%)       7 (13%)      1 (2%)                                
      Mineralization                                                1 (2%)       1 (2%)                                             
      Necrosis                                                                   1 (2%)                                             
      Ulcer                                            4 (7%)       3 (5%)       7 (13%)      3 (5%)                                
   Stomach, Glandular                                 (56)         (54)         (56)         (55)                                   
      Hemorrhage                                                                 1 (2%)                                             
      Inflammation, Suppurative                                                  1 (2%)                                             
      Mineralization                                                1 (2%)       1 (2%)       1 (2%)                                
      Necrosis                                                      1 (2%)       1 (2%)                                             
      Ulcer                                            1 (2%)                    1 (2%)                                             
   Tongue                                                          (2)                       (1)                                    
      Epithelium, Hyperplasia                                       1 (50%)                                                         
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Blood Vessel                                       (56)         (54)         (56)         (56)                                   
      Mineralization                                   1 (2%)                                                                       
      Aorta, Inflammation                              1 (2%)       1 (2%)                                                          
      Aorta, Mineralization                                         2 (4%)       1 (2%)                                             
   Heart                                              (56)         (55)         (56)         (56)                                   
      Cardiomyopathy                                  14 (25%)     11 (20%)     12 (21%)     11 (20%)                               
      Atrium, Thrombosis                               8 (14%)      3 (5%)       3 (5%)       1 (2%)                                
      Myocardium, Mineralization                                    1 (2%)       1 (2%)                                             
      Myocardium, Necrosis                                                       1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (56)         (55)         (56)         (56)                                   
      Hemorrhage                                                    1 (2%)       2 (4%)                                             
      Hyperplasia                                                                1 (2%)                                             
      Vacuolization Cytoplasmic                        7 (13%)      8 (15%)     10 (18%)      7 (13%)                               
   Adrenal Medulla                                    (56)         (53)         (53)         (56)                                   
      Hyperplasia                                     20 (36%)     16 (30%)     21 (40%)     16 (29%)                               
   Islets, Pancreatic                                 (56)         (55)         (56)         (56)                                   
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    9                                                              
NTP Experiment-Test: 05133-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                            ACETONITRILE                                       Date: 05/20/95  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 06:11:11  
____________________________________________________________________________________________________________________________________
         FISCHER 344 RATS MALE                     CONTROL      100 PPM      200 PPM      400 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ENDOCRINE SYSTEM - CONT                                                                                                             
      Hyperplasia                                      1 (2%)                    2 (4%)                                             
   Parathyroid Gland                                  (54)         (49)         (54)         (49)                                   
      Hyperplasia                                      2 (4%)       5 (10%)      4 (7%)       2 (4%)                                
   Pituitary Gland                                    (56)         (53)         (56)         (54)                                   
      Cyst                                             3 (5%)       7 (13%)      8 (14%)      4 (7%)                                
      Hemorrhage                                       1 (2%)       2 (4%)       1 (2%)                                             
      Pars Distalis, Hemorrhage                        1 (2%)                                                                       
      Pars Distalis, Hyperplasia                       8 (14%)      9 (17%)      9 (16%)     10 (19%)                               
      Pars Intermedia, Hyperplasia                                  1 (2%)                                                          
   Thyroid Gland                                      (56)         (55)         (56)         (56)                                   
      Ultimobranchial Cyst                                                       2 (4%)                                             
      C-Cell, Hyperplasia                             10 (18%)     10 (18%)      9 (16%)     12 (21%)                               
      Follicle, Dilatation                             1 (2%)                                                                       
      Follicular Cell, Hyperplasia                     1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Epididymis                                         (56)         (55)         (56)         (56)                                   
      Inflammation, Suppurative                                                  1 (2%)                                             
   Preputial Gland                                    (55)         (55)         (55)         (56)                                   
      Ectasia                                         22 (40%)     16 (29%)     25 (45%)     16 (29%)                               
      Granuloma                                                     1 (2%)                                                          
      Hyperplasia                                      1 (2%)       2 (4%)       2 (4%)       3 (5%)                                
      Inflammation, Chronic                            1 (2%)                    4 (7%)                                             
      Inflammation, Suppurative                        7 (13%)      7 (13%)      6 (11%)      8 (14%)                               
   Prostate                                           (56)         (55)         (56)         (56)                                   
      Cyst                                                          1 (2%)                                                          
      Inflammation, Suppurative                        9 (16%)     10 (18%)      9 (16%)     12 (21%)                               
      Epithelium, Hyperplasia                          1 (2%)       1 (2%)       1 (2%)       2 (4%)                                
   Seminal Vesicle                                    (56)         (54)         (56)         (56)                                   
      Hyperplasia                                                   1 (2%)       1 (2%)       1 (2%)                                
      Inflammation, Suppurative                        1 (2%)       2 (4%)       2 (4%)                                             
   Testes                                             (56)         (55)         (56)         (56)                                   
      Hemorrhage                                       1 (2%)       1 (2%)                                                          
      Inflammation, Suppurative                        1 (2%)                                                                       
      Necrosis                                         1 (2%)                                                                       
      Germinal Epithelium, Atrophy                    10 (18%)     13 (24%)      9 (16%)      9 (16%)                               
      Interstitial Cell, Hyperplasia                  21 (38%)     19 (35%)     21 (38%)      9 (16%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   10                                                              
NTP Experiment-Test: 05133-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                            ACETONITRILE                                       Date: 05/20/95  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 06:11:11  
____________________________________________________________________________________________________________________________________
         FISCHER 344 RATS MALE                     CONTROL      100 PPM      200 PPM      400 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (56)         (55)         (56)         (56)                                   
      Hyperplasia, Re Cell                             7 (13%)                   1 (2%)                                             
      Myelofibrosis                                    2 (4%)                                                                       
   Lymph Node                                         (12)         (8)          (15)         (13)                                   
      Inflammation, Granulomatous                      1 (8%)                                                                       
      Iliac, Pigmentation                                                                     1 (8%)                                
      Lumbar, Pigmentation                             1 (8%)       1 (13%)      2 (13%)      1 (8%)                                
      Pancreatic, Hyperplasia, Lymphoid                                                       1 (8%)                                
      Renal, Ectasia                                                1 (13%)                                                         
      Renal, Hemorrhage                                                                       1 (8%)                                
      Renal, Pigmentation                                                        1 (7%)       1 (8%)                                
   Lymph Node, Mandibular                             (49)         (51)         (52)         (54)                                   
      Hemorrhage                                                    1 (2%)                    1 (2%)                                
      Hyperplasia, Lymphoid                                         2 (4%)       1 (2%)                                             
   Lymph Node, Mesenteric                             (55)         (55)         (55)         (56)                                   
      Hemorrhage                                       1 (2%)       1 (2%)                    1 (2%)                                
      Hyperplasia, Lymphoid                                                      1 (2%)                                             
      Inflammation, Granulomatous                                   1 (2%)                                                          
   Lymph Node, Mediastinal                            (56)         (53)         (56)         (56)                                   
      Fibrosis                                                                   3 (5%)                                             
      Hemorrhage                                       1 (2%)       5 (9%)       1 (2%)       1 (2%)                                
      Hyperplasia, Lymphoid                                                      1 (2%)                                             
      Pigmentation                                     1 (2%)       1 (2%)                                                          
   Spleen                                             (56)         (55)         (56)         (56)                                   
      Congestion                                                                              1 (2%)                                
      Developmental Malformation                                    2 (4%)       2 (4%)       2 (4%)                                
      Fibrosis                                         7 (13%)     13 (24%)     18 (32%)     15 (27%)                               
      Hemorrhage                                       1 (2%)                                                                       
      Hyperplasia, Re Cell                             4 (7%)       1 (2%)                                                          
      Necrosis                                                      1 (2%)       2 (4%)                                             
   Thymus                                             (55)         (52)         (54)         (56)                                   
      Hemorrhage                                                                 1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (55)         (55)         (56)         (56)                                   
      Galactocele                                      1 (2%)       2 (4%)       3 (5%)                                             
      Hemorrhage                                                                 1 (2%)                                             
      Inflammation, Chronic                                         1 (2%)                                                          
      Epithelium, Hyperplasia                                       1 (2%)       3 (5%)       1 (2%)                                
   Skin                                               (56)         (55)         (56)         (56)                                   
      Acanthosis                                       2 (4%)       2 (4%)       3 (5%)                                             
      Cyst                                                          1 (2%)                                                          
      Cyst Epithelial Inclusion                                     2 (4%)       1 (2%)       1 (2%)                                
      Hyperkeratosis                                   1 (2%)       2 (4%)       1 (2%)                                             
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   11                                                              
NTP Experiment-Test: 05133-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                            ACETONITRILE                                       Date: 05/20/95  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 06:11:11  
____________________________________________________________________________________________________________________________________
         FISCHER 344 RATS MALE                     CONTROL      100 PPM      200 PPM      400 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
INTEGUMENTARY SYSTEM - CONT                                                                                                         
      Inflammation, Chronic                                         1 (2%)                                                          
      Inflammation, Granulomatous                      2 (4%)       1 (2%)       3 (5%)                                             
      Inflammation, Suppurative                                                  1 (2%)                                             
      Ulcer                                            4 (7%)       1 (2%)       1 (2%)       1 (2%)                                
      Subcutaneous Tissue, Hemorrhage                               1 (2%)       1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (56)         (55)         (56)         (56)                                   
      Developmental Malformation                                    1 (2%)                                                          
      Fibrosis                                                      1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (56)         (55)         (56)         (56)                                   
      Demyelination                                                 1 (2%)                                                          
      Gliosis                                          1 (2%)                                                                       
      Hemorrhage                                       3 (5%)       5 (9%)       6 (11%)      4 (7%)                                
      Hydrocephalus                                    7 (13%)      7 (13%)      7 (13%)      2 (4%)                                
      Necrosis                                                      1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Larynx                                             (56)         (54)         (56)         (56)                                   
      Foreign Body                                     4 (7%)       4 (7%)       5 (9%)       9 (16%)                               
      Hyperplasia                                      1 (2%)       4 (7%)       1 (2%)       4 (7%)                                
      Inflammation, Suppurative                        5 (9%)       3 (6%)       4 (7%)       8 (14%)                               
      Metaplasia, Squamous                             1 (2%)       2 (4%)       1 (2%)                                             
      Mineralization                                                1 (2%)                    1 (2%)                                
   Lung                                               (56)         (55)         (56)         (56)                                   
      Embolus                                          1 (2%)                                                                       
      Hemorrhage                                      24 (43%)     37 (67%)     34 (61%)     35 (63%)                               
      Infarct                                                                    1 (2%)                                             
      Infiltration Cellular, Histiocyte               10 (18%)     16 (29%)     14 (25%)     11 (20%)                               
      Metaplasia, Osseous                                           1 (2%)                                                          
      Thrombosis                                                                 1 (2%)                                             
      Alveolar Epithelium, Hyperplasia                 3 (5%)       7 (13%)      4 (7%)      10 (18%)                               
      Alveolus, Fibrosis                               2 (4%)       1 (2%)       7 (13%)                                            
      Alveolus, Inflammation, Chronic                  4 (7%)                                 2 (4%)                                
      Alveolus, Mineralization                                      1 (2%)       1 (2%)                                             
      Alveolus, Pigmentation                                        1 (2%)       1 (2%)                                             
      Artery, Inflammation, Chronic Active                                       1 (2%)                                             
      Pleura, Fibrosis                                 1 (2%)                                 1 (2%)                                
   Nose                                               (56)         (55)         (56)         (56)                                   
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   12                                                              
NTP Experiment-Test: 05133-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                            ACETONITRILE                                       Date: 05/20/95  
Route: RESPIRATORY EXPOSURE WHOLE BODY                                                                            Time: 06:11:11  
____________________________________________________________________________________________________________________________________
         FISCHER 344 RATS MALE                     CONTROL      100 PPM      200 PPM      400 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
RESPIRATORY SYSTEM - CONT                                                                                                           
      Foreign Body                                    10 (18%)      9 (16%)      7 (13%)      7 (13%)                               
      Hemorrhage                                       6 (11%)      3 (5%)       2 (4%)       5 (9%)                                
      Inflammation, Chronic                            2 (4%)       2 (4%)       3 (5%)       2 (4%)                                
      Inflammation, Suppurative                        4 (7%)       4 (7%)       6 (11%)     10 (18%)                               
      Goblet Cell, Respiratory Epithelium,                                                                                          
          Hypertrophy                                  9 (16%)      4 (7%)       8 (14%)      3 (5%)                                
      Nasolacrimal Duct, Inflammation                                                         1 (2%)                                
      Nasopharyngeal Duct, Hyperplasia                                                        1 (2%)                                
      Olfactory Epithelium, Metaplasia                              1 (2%)                                                          
      Respiratory Epithelium, Hyperplasia              5 (9%)       5 (9%)       6 (11%)      8 (14%)                               
   Trachea                                            (56)         (55)         (56)         (56)                                   
      Peritracheal Tissue, Fibrosis                                                           1 (2%)                                
      Peritracheal Tissue, Inflammation, Chronic                                                                                    
          Active                                                                              1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                (1)          (1)          (2)          (2)                                    
      Cataract                                                      1 (100%)                  2 (100%)                              
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (56)         (55)         (56)         (56)                                   
      Nephropathy, Chronic                            52 (93%)     55 (100%)    55 (98%)     56 (100%)                              
      Thrombosis                                       1 (2%)                                                                       
      Cortex, Necrosis                                 1 (2%)       1 (2%)       3 (5%)                                             
      Pelvis, Dilatation                                            1 (2%)                    1 (2%)                                
      Pelvis, Transitional Epithelium, Hyperplasia     3 (5%)       3 (5%)       1 (2%)       1 (2%)                                
      Renal Tubule, Hyperplasia                        1 (2%)                    1 (2%)                                             
      Renal Tubule, Mineralization                                  1 (2%)       1 (2%)                                             
   Urinary Bladder                                    (56)         (55)         (56)         (56)                                   
      Hemorrhage                                       2 (4%)       1 (2%)                                                          
      Inflammation, Suppurative                                     1 (2%)                                                          
      Metaplasia, Squamous                             1 (2%)                                                                       
      Mineralization                                                1 (2%)                                                          
      Ulcer                                            1 (2%)                                                                       
      Transitional Epithelium, Hyperplasia                          2 (4%)                    2 (4%)                                
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   13                                                              
                             ------------------------------------------------------------                                           
                             ----------              END OF REPORT             ----------                                           
                             ------------------------------------------------------------                                           
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.