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TDMS Study 05205-08 Pathology Tables

NTP Experiment-Test: 05205-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           P-NITROTOLUENE                                      Date: 02/08/00
Route: DOSED FEED                                                                                                 Time: 09:18:07

                                                 FINAL#1 CHRONIC STUDY FOR MICE




       Facility:  Southern Research Institute

       Chemical CAS #:  99-99-0

       Lock Date:  08/24/98

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All


































a  Number of animals examined microscopically at site and number of animals with lesion

                                                              Page   1


NTP Experiment-Test: 05205-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           P-NITROTOLUENE                                      Date: 02/08/00  
Route: DOSED FEED                                                                                                 Time: 09:18:07  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE FEMALE                        0            1250         2500         5000                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________

DISPOSITION SUMMARY                                                                                                                 


  Animals Initially In Study                          50           50           50           50                                     
  Early Deaths                                                                                                                      
    Moribund Sacrifice                                 2            1            5            1                                     
    Natural Death                                      2            2            2                                                  
  Survivors                                                                                                                         
    Terminal Sacrifice                                46           47           41           49                                     
    Natural Death                                                                2                                                  

  Animals Examined Microscopically                    50           50           50           50                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Gallbladder                                        (49)         (48)         (48)         (49)                                   
      Inflammation, Chronic                                                                   1 (2%)                                
   Intestine Small, Duodenum                          (49)         (49)         (49)         (50)                                   
      Inflammation, Chronic, Focal                                                            1 (2%)                                
      Epithelium, Cyst                                              1 (2%)                                                          
   Intestine Small, Jejunum                           (49)         (48)         (48)         (50)                                   
      Inflammation, Focal                                                        1 (2%)                                             
      Peyer's Patch, Hyperplasia, Lymphoid             1 (2%)                                                                       
      Serosa, Cyst                                                  1 (2%)                                                          
   Intestine Small, Ileum                             (49)         (48)         (48)         (50)                                   
      Peyer's Patch, Hyperplasia, Lymphoid             2 (4%)                                                                       
   Liver                                              (49)         (50)         (50)         (50)                                   
      Angiectasis                                      1 (2%)                                                                       
      Atrophy, Focal                                                                          1 (2%)                                
      Basophilic Focus                                 2 (4%)       3 (6%)                                                          
      Congestion, Focal                                                                       1 (2%)                                
      Eosinophilic Focus                                            3 (6%)                                                          
      Hemorrhage, Focal                                                                       1 (2%)                                
      Hyperplasia, Focal, Lymphoid                     1 (2%)       1 (2%)       1 (2%)                                             
      Infarct                                                       1 (2%)                                                          
      Infiltration Cellular, Mixed Cell               40 (82%)     41 (82%)     39 (78%)     39 (78%)                               
      Inflammation, Chronic                            1 (2%)       1 (2%)                    1 (2%)                                
      Mixed Cell Focus                                              1 (2%)                                                          
      Tension Lipidosis                                             1 (2%)                                                          
      Artery, Inflammation, Chronic                                                           1 (2%)                                
      Bile Duct, Cyst                                                            1 (2%)                                             
      Centrilobular, Necrosis                                                    1 (2%)                                             
      Hepatocyte, Fatty Change, Diffuse                                          1 (2%)                                             
      Hepatocyte, Necrosis, Focal                                   2 (4%)                    1 (2%)                                
      Hepatocyte, Vacuolization Cytoplasmic, Focal                  1 (2%)                                                          
      Hepatocyte, Periportal, Vacuolization                                                                                         
          Cytoplasmic                                                                         3 (6%)                                
      Portal, Inflammation, Chronic                                                           1 (2%)                                

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 05205-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           P-NITROTOLUENE                                      Date: 02/08/00  
Route: DOSED FEED                                                                                                 Time: 09:18:07  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE FEMALE                        0            1250         2500         5000                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
      Serosa, Inflammation, Chronic                                                           1 (2%)                                
   Mesentery                                          (3)          (3)          (4)          (7)                                    
      Inflammation, Chronic                                         2 (67%)      1 (25%)      1 (14%)                               
      Artery, Inflammation, Chronic                    1 (33%)                                1 (14%)                               
      Fat, Necrosis                                    1 (33%)      1 (33%)      1 (25%)      1 (14%)                               
   Pancreas                                           (49)         (50)         (49)         (50)                                   
      Acinus, Atrophy, Diffuse                         2 (4%)                                                                       
      Acinus, Atrophy, Focal                           1 (2%)                                 1 (2%)                                
      Duct, Cyst                                       3 (6%)                    1 (2%)                                             
   Stomach, Forestomach                               (49)         (50)         (50)         (50)                                   
      Diverticulum                                                  5 (10%)      1 (2%)                                             
      Epithelium, Hyperplasia                                       2 (4%)                                                          
   Stomach, Glandular                                 (49)         (50)         (49)         (50)                                   
      Erosion                                                       1 (2%)                                                          
   Tooth                                              (3)          (6)          (7)          (6)                                    
      Malformation                                                               1 (14%)                                            
      Peridontal Tissue, Inflammation, Chronic         3 (100%)     6 (100%)     6 (86%)      6 (100%)                              
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Blood Vessel                                       (1)                                                                           
      Inflammation, Chronic                            1 (100%)                                                                     
   Heart                                              (50)         (50)         (50)         (50)                                   
      Infiltration Cellular, Mixed Cell                4 (8%)       2 (4%)       1 (2%)       6 (12%)                               
      Inflammation, Chronic, Focal                     1 (2%)                                                                       
      Artery, Inflammation, Chronic                    1 (2%)                    1 (2%)       1 (2%)                                
      Epicardium, Infiltration Cellular, Mixed Cell    2 (4%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (50)         (50)         (50)         (50)                                   
      Accessory Adrenal Cortical Nodule                             1 (2%)                                                          
      Cyst                                             1 (2%)                                 1 (2%)                                
      Cytoplasmic Alteration, Focal                                 1 (2%)                                                          
      Hemorrhage                                                                 1 (2%)                                             
   Adrenal Medulla                                    (50)         (50)         (50)         (50)                                   
      Hyperplasia                                      1 (2%)                                                                       
   Parathyroid Gland                                  (49)         (48)         (44)         (48)                                   
      Cyst                                                                       1 (2%)                                             
   Pituitary Gland                                    (45)         (49)         (49)         (45)                                   
      Angiectasis                                                   1 (2%)                                                          
      Pars Distalis, Angiectasis                                    1 (2%)                                                          
      Pars Distalis, Cyst                                                        1 (2%)                                             
      Pars Distalis, Cytoplasmic Alteration, Focal     4 (9%)       2 (4%)       3 (6%)                                             
      Pars Distalis, Hyperplasia, Focal                             2 (4%)                                                          

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 05205-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           P-NITROTOLUENE                                      Date: 02/08/00  
Route: DOSED FEED                                                                                                 Time: 09:18:07  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE FEMALE                        0            1250         2500         5000                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ENDOCRINE SYSTEM - CONT                                                                                                             
   Thyroid Gland                                      (50)         (50)         (49)         (50)                                   
      Degeneration, Cystic, Focal                     18 (36%)     27 (54%)     29 (59%)     22 (44%)                               
      Follicle, Cyst                                   1 (2%)       1 (2%)       1 (2%)                                             
      Follicular Cell, Hyperplasia                     1 (2%)       3 (6%)       1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   Tissue NOS                                                                                (2)                                    
      Abdominal, Inflammation, Chronic                                                        1 (50%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Clitoral Gland                                     (50)         (45)         (49)         (48)                                   
      Degeneration, Cystic                             1 (2%)                    2 (4%)                                             
      Inflammation, Chronic                                         1 (2%)                                                          
      Pigmentation                                     1 (2%)       4 (9%)       3 (6%)       2 (4%)                                
   Ovary                                              (48)         (50)         (50)         (49)                                   
      Angiectasis                                      1 (2%)       2 (4%)                                                          
      Cyst                                            11 (23%)     13 (26%)     13 (26%)     13 (27%)                               
      Cyst, Multiple                                                1 (2%)                                                          
      Hemorrhage                                       1 (2%)       2 (4%)       5 (10%)                                            
      Mineralization                                   1 (2%)                                                                       
      Bilateral, Cyst                                  1 (2%)                                                                       
      Periovarian Tissue, Cyst                         1 (2%)                                                                       
   Uterus                                             (50)         (50)         (50)         (50)                                   
      Angiectasis                                                   1 (2%)                                                          
      Cyst                                             1 (2%)                    4 (8%)       3 (6%)                                
      Hemorrhage                                       2 (4%)                                 1 (2%)                                
      Hydrometra                                      19 (38%)     21 (42%)     23 (46%)     25 (50%)                               
      Inflammation, Suppurative                        1 (2%)                                                                       
      Thrombosis                                                    1 (2%)                                                          
      Endometrium, Hyperplasia, Cystic                47 (94%)     45 (90%)     44 (88%)     49 (98%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (50)         (50)         (50)         (50)                                   
      Hyperplasia                                      1 (2%)       1 (2%)                                                          
      Hyperplasia, Focal, Histiocytic                                                         1 (2%)                                
   Lymph Node                                         (2)          (5)          (13)         (4)                                    
      Bronchial, Hyperplasia                                        1 (20%)      2 (15%)                                            
      Bronchial, Hyperplasia, Lymphoid                                           3 (23%)      1 (25%)                               
      Iliac, Hyperplasia, Lymphoid                     1 (50%)                                                                      
      Inguinal, Hyperplasia, Histiocytic                                         1 (8%)                                             
      Inguinal, Hyperplasia, Lymphoid                                            1 (8%)                                             

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 05205-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           P-NITROTOLUENE                                      Date: 02/08/00  
Route: DOSED FEED                                                                                                 Time: 09:18:07  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE FEMALE                        0            1250         2500         5000                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - CONT                                                                                                         
      Inguinal, Pigmentation                                                                  1 (25%)                               
      Mediastinal, Hyperplasia, Lymphoid                                                      1 (25%)                               
      Pancreatic, Hyperplasia, Lymphoid                             1 (20%)                                                         
      Renal, Hyperplasia, Lymphoid                     1 (50%)      1 (20%)                                                         
   Lymph Node, Mandibular                             (50)         (48)         (48)         (47)                                   
      Hyperplasia                                      1 (2%)                                                                       
      Hyperplasia, Lymphoid                                         1 (2%)       2 (4%)                                             
   Lymph Node, Mesenteric                             (48)         (49)         (49)         (50)                                   
      Hyperplasia                                                                1 (2%)       2 (4%)                                
      Hyperplasia, Histiocytic                                      1 (2%)       1 (2%)                                             
      Hyperplasia, Lymphoid                            2 (4%)       5 (10%)                                                         
   Spleen                                             (49)         (50)         (49)         (50)                                   
      Congestion                                                    1 (2%)                                                          
      Hematopoietic Cell Proliferation                 2 (4%)       7 (14%)     11 (22%)      4 (8%)                                
      Hyperplasia, Lymphoid                            5 (10%)      9 (18%)      4 (8%)       2 (4%)                                
   Thymus                                             (48)         (49)         (47)         (46)                                   
      Angiectasis                                      1 (2%)       2 (4%)                                                          
      Cyst                                             1 (2%)       1 (2%)       2 (4%)                                             
      Hyperplasia, Lymphoid                                                      2 (4%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (50)         (50)         (50)         (50)                                   
      Ectasia                                                                    2 (4%)                                             
      Hyperplasia                                                                1 (2%)                                             
   Skin                                               (50)         (50)         (50)         (50)                                   
      Subcutaneous Tissue, Angiectasis, Focal                                    1 (2%)                                             
      Subcutaneous Tissue, Cyst Epithelial                                                                                          
          Inclusion                                    1 (2%)                                                                       
      Subcutaneous Tissue, Degeneration, Mucoid        1 (2%)       1 (2%)                                                          
      Subcutaneous Tissue, Edema                                    1 (2%)                                                          
      Subcutaneous Tissue, Inflammation, Chronic,                                                                                   
           Focal                                                    2 (4%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (50)         (50)         (50)         (50)                                   
      Callus                                           1 (2%)                                                                       
   Skeletal Muscle                                    (1)                       (3)          (1)                                    
      Artery, Inflammation, Chronic                    1 (100%)                                                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 05205-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           P-NITROTOLUENE                                      Date: 02/08/00  
Route: DOSED FEED                                                                                                 Time: 09:18:07  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE FEMALE                        0            1250         2500         5000                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (50)         (50)         (50)         (50)                                   
      Atrophy, Focal                                                1 (2%)                                                          
      Meninges, Hyperplasia, Lymphoid                                            1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (50)         (50)         (50)                                   
      Congestion                                                                              1 (2%)                                
      Hemorrhage                                       1 (2%)       5 (10%)      2 (4%)       5 (10%)                               
      Hyperplasia, Histiocytic                                                   2 (4%)                                             
      Hyperplasia, Lymphoid                                                      1 (2%)                                             
      Infiltration Cellular, Mixed Cell                             1 (2%)                                                          
      Thrombosis, Chronic                              1 (2%)                                                                       
      Alveolar Epithelium, Bronchiolization                        33 (66%)     41 (82%)     49 (98%)                               
      Alveolar Epithelium, Hyperplasia                 2 (4%)       1 (2%)       2 (4%)       1 (2%)                                
   Nose                                               (50)         (50)         (50)         (50)                                   
      Hemorrhage                                                                 1 (2%)                                             
      Inflammation, Suppurative                        1 (2%)       1 (2%)                                                          
      Nasolacrimal Duct, Inflammation                                                         1 (2%)                                
      Squamous Epithelium, Nasolacrimal Duct,                                                                                       
          Hyperplasia, Focal                                                                  1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (49)         (50)         (50)         (50)                                   
      Congestion                                                                 1 (2%)                                             
      Hyperplasia, Lymphoid                            1 (2%)                    2 (4%)                                             
      Infiltration Cellular, Mixed Cell                1 (2%)                                                                       
      Metaplasia, Focal, Osseous                       1 (2%)       1 (2%)       1 (2%)                                             
      Nephropathy                                     11 (22%)      7 (14%)      9 (18%)      3 (6%)                                
      Renal Tubule, Accumulation, Hyaline Droplet                   1 (2%)       4 (8%)                                             
      Renal Tubule, Casts Protein                                                1 (2%)                                             
      Renal Tubule, Pigmentation                       1 (2%)                                                                       
   Urinary Bladder                                    (49)         (50)         (50)         (50)                                   

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 05205-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           P-NITROTOLUENE                                      Date: 02/08/00  
Route: DOSED FEED                                                                                                 Time: 09:18:07  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE FEMALE                        0            1250         2500         5000                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
URINARY SYSTEM - CONT                                                                                                               
      Hyperplasia, Lymphoid                                                      1 (2%)                                             
                                                                                                                                    

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 05205-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           P-NITROTOLUENE                                      Date: 02/08/00  
Route: DOSED FEED                                                                                                 Time: 09:18:07  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE MALE                          0            1250         2500         5000                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________

DISPOSITION SUMMARY                                                                                                                 


  Animals Initially In Study                          50           50           50           50                                     
  Early Deaths                                                                                                                      
    Moribund Sacrifice                                 2            2            3            6                                     
    Natural Death                                      2            2            2            2                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                46           46           45           42                                     

  Animals Examined Microscopically                    50           50           50           50                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Gallbladder                                        (49)         (48)         (48)         (47)                                   
      Inflammation, Chronic                            1 (2%)                                                                       
      Epithelium, Hyperplasia                          1 (2%)                                                                       
   Intestine Small, Duodenum                          (48)         (49)         (49)         (49)                                   
      Ectasia                                                                                 1 (2%)                                
      Inflammation, Chronic, Focal                                                            1 (2%)                                
      Necrosis, Focal                                                                         1 (2%)                                
      Epithelium, Hyperplasia, Focal                                                          1 (2%)                                
   Intestine Small, Jejunum                           (48)         (49)         (48)         (49)                                   
      Epithelium, Hyperplasia, Focal                   1 (2%)                                                                       
      Peyer's Patch, Hyperplasia, Lymphoid                                                    2 (4%)                                
      Peyer's Patch, Inflammation, Chronic,                                                                                         
          Suppurative                                               1 (2%)                                                          
   Intestine Small, Ileum                             (48)         (49)         (48)         (49)                                   
      Epithelium, Cyst                                 1 (2%)                                                                       
      Peyer's Patch, Hyperplasia, Lymphoid                                                    3 (6%)                                
   Liver                                              (50)         (50)         (50)         (50)                                   
      Basophilic Focus                                 4 (8%)       5 (10%)                   1 (2%)                                
      Basophilic Focus, Multiple                       1 (2%)                                 1 (2%)                                
      Clear Cell Focus                                 4 (8%)       5 (10%)      6 (12%)      9 (18%)                               
      Clear Cell Focus, Multiple                                    1 (2%)       1 (2%)                                             
      Cyst                                                          1 (2%)                    1 (2%)                                
      Eosinophilic Focus                               3 (6%)       3 (6%)       2 (4%)       6 (12%)                               
      Infiltration Cellular, Mixed Cell               30 (60%)     30 (60%)     26 (52%)     33 (66%)                               
      Inflammation, Chronic                            1 (2%)                                                                       
      Mixed Cell Focus                                 1 (2%)       1 (2%)       2 (4%)       3 (6%)                                
      Pigmentation, Focal                                           1 (2%)                                                          
      Thrombosis                                       1 (2%)                                                                       
      Bile Duct, Hyperplasia                                                     1 (2%)       1 (2%)                                
      Centrilobular, Necrosis                                                    1 (2%)                                             
      Hepatocyte, Fatty Change, Diffuse                             1 (2%)                                                          
      Hepatocyte, Necrosis, Focal                      4 (8%)       3 (6%)       4 (8%)       3 (6%)                                
      Hepatocyte, Syncytial Alteration, Focal          2 (4%)      13 (26%)     17 (34%)     33 (66%)                               
      Hepatocyte, Vacuolization Cytoplasmic,                                                                                        

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 05205-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           P-NITROTOLUENE                                      Date: 02/08/00  
Route: DOSED FEED                                                                                                 Time: 09:18:07  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE MALE                          0            1250         2500         5000                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
          Diffuse                                      1 (2%)                                                                       
      Hepatocyte, Vacuolization Cytoplasmic, Focal     1 (2%)                                 1 (2%)                                
      Hepatocyte, Centrilobular, Fatty Change                       1 (2%)                                                          
      Serosa, Inflammation, Chronic                                              1 (2%)                                             
   Mesentery                                          (6)          (7)          (8)          (5)                                    
      Hemorrhage                                                                 1 (13%)                                            
      Inflammation, Chronic                            1 (17%)      1 (14%)      1 (13%)      2 (40%)                               
      Fat, Necrosis                                    4 (67%)      4 (57%)      4 (50%)      2 (40%)                               
   Oral Mucosa                                                                               (1)                                    
      Inflammation, Chronic                                                                   1 (100%)                              
   Pancreas                                           (50)         (50)         (50)         (50)                                   
      Angiectasis                                                                             1 (2%)                                
      Inflammation, Chronic                                                      1 (2%)                                             
      Lipomatosis                                                                1 (2%)                                             
      Acinus, Atrophy, Diffuse                                                   1 (2%)                                             
      Acinus, Cytoplasmic Alteration                                1 (2%)                                                          
      Duct, Cyst                                       1 (2%)                    1 (2%)                                             
   Stomach, Forestomach                               (50)         (50)         (50)         (50)                                   
      Diverticulum                                                               1 (2%)                                             
      Edema                                                         1 (2%)                                                          
      Inflammation, Focal                              1 (2%)       1 (2%)                                                          
      Ulcer                                                         1 (2%)       1 (2%)                                             
      Epithelium, Cyst                                              1 (2%)                                                          
      Epithelium, Hyperplasia                          3 (6%)       5 (10%)                   2 (4%)                                
   Stomach, Glandular                                 (49)         (49)         (50)         (50)                                   
      Foreign Body                                                                            1 (2%)                                
      Inflammation, Focal                                                                     1 (2%)                                
      Pigmentation, Focal                                                        1 (2%)                                             
      Epithelium, Cytoplasmic Alteration, Focal                                               1 (2%)                                
      Glands, Degeneration, Cystic, Focal              1 (2%)                                 1 (2%)                                
   Tooth                                              (6)          (13)         (8)          (8)                                    
      Malformation                                     1 (17%)      1 (8%)                    2 (25%)                               
      Peridontal Tissue, Inflammation, Chronic         5 (83%)     13 (100%)     8 (100%)     6 (75%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (50)         (50)         (50)         (50)                                   
      Infiltration Cellular, Mixed Cell                2 (4%)       1 (2%)       3 (6%)       3 (6%)                                
      Inflammation, Chronic, Focal                     2 (4%)       1 (2%)                                                          
      Mineralization                                                             1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 05205-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           P-NITROTOLUENE                                      Date: 02/08/00  
Route: DOSED FEED                                                                                                 Time: 09:18:07  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE MALE                          0            1250         2500         5000                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (49)         (50)         (49)         (50)                                   
      Accessory Adrenal Cortical Nodule                             2 (4%)                                                          
      Cyst                                             1 (2%)       1 (2%)                                                          
      Cytoplasmic Alteration, Focal                    1 (2%)       5 (10%)      9 (18%)      2 (4%)                                
      Hypertrophy                                                                             1 (2%)                                
      Hypertrophy, Focal                               4 (8%)                                 4 (8%)                                
      Infiltration Cellular, Mixed Cell                1 (2%)                                                                       
      Capsule, Hyperplasia, Focal                      1 (2%)       6 (12%)      1 (2%)       1 (2%)                                
   Adrenal Medulla                                    (49)         (50)         (49)         (49)                                   
      Hyperplasia                                                   1 (2%)       1 (2%)                                             
   Parathyroid Gland                                  (48)         (45)         (44)         (50)                                   
      Cyst                                                                                    3 (6%)                                
   Pituitary Gland                                    (46)         (49)         (49)         (45)                                   
      Pars Distalis, Cyst                              2 (4%)       1 (2%)       1 (2%)                                             
      Pars Distalis, Hyperplasia, Focal                             1 (2%)                                                          
      Pars Intermedia, Hyperplasia, Focal                                        1 (2%)                                             
   Thyroid Gland                                      (50)         (50)         (49)         (49)                                   
      Degeneration, Cystic, Focal                     10 (20%)      9 (18%)     13 (27%)     15 (31%)                               
      Follicle, Cyst                                                1 (2%)                                                          
      Follicular Cell, Hyperplasia                                  1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Epididymis                                         (50)         (50)         (49)         (50)                                   
      Inflammation, Chronic                                                      1 (2%)       1 (2%)                                
   Preputial Gland                                    (50)         (50)         (50)         (50)                                   
      Degeneration, Cystic                            29 (58%)     29 (58%)     32 (64%)     28 (56%)                               
      Inflammation, Chronic                            3 (6%)       2 (4%)       1 (2%)                                             
      Bilateral, Degeneration, Cystic                               2 (4%)                                                          
   Seminal Vesicle                                    (50)         (50)         (50)         (50)                                   
      Dilatation                                       1 (2%)       1 (2%)                                                          
      Inflammation, Chronic                                         1 (2%)                                                          
   Testes                                             (50)         (50)         (50)         (50)                                   
      Cyst                                             1 (2%)                                                                       
      Germinal Epithelium, Degeneration                                          3 (6%)       1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 05205-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           P-NITROTOLUENE                                      Date: 02/08/00  
Route: DOSED FEED                                                                                                 Time: 09:18:07  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE MALE                          0            1250         2500         5000                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (50)         (50)         (50)         (50)                                   
      Angiectasis                                                                             1 (2%)                                
      Hyperplasia                                                                1 (2%)       1 (2%)                                
      Hyperplasia, Focal, Histiocytic                                                         1 (2%)                                
      Inflammation, Chronic, Focal                     1 (2%)                                                                       
      Necrosis, Focal                                                                         1 (2%)                                
   Lymph Node                                         (5)          (5)          (3)          (5)                                    
      Bronchial, Hyperplasia                           1 (20%)                                                                      
      Bronchial, Hyperplasia, Lymphoid                              1 (20%)      1 (33%)                                            
      Inguinal, Hyperplasia, Lymphoid                  1 (20%)                                                                      
      Mediastinal, Hyperplasia                                                                1 (20%)                               
      Mediastinal, Hyperplasia, Lymphoid                            1 (20%)                   1 (20%)                               
      Mediastinal, Infiltration Cellular, Lipocyte                  1 (20%)                                                         
      Mediastinal, Pigmentation                        1 (20%)                                                                      
      Pancreatic, Amyloid Deposition                                                          1 (20%)                               
      Pancreatic, Hyperplasia                                                                 1 (20%)                               
      Renal, Hyperplasia                                                                      1 (20%)                               
   Lymph Node, Mandibular                             (48)         (50)         (45)         (48)                                   
      Hyperplasia                                      1 (2%)                                                                       
      Hyperplasia, Lymphoid                            1 (2%)                    1 (2%)       1 (2%)                                
   Lymph Node, Mesenteric                             (50)         (49)         (49)         (49)                                   
      Hemorrhage                                                    1 (2%)                                                          
      Hyperplasia                                                   1 (2%)                    1 (2%)                                
      Hyperplasia, Lymphoid                                                                   1 (2%)                                
      Inflammation, Chronic, Suppurative                            1 (2%)                                                          
   Spleen                                             (50)         (50)         (50)         (50)                                   
      Angiectasis                                      1 (2%)                                                                       
      Congestion                                       1 (2%)       1 (2%)                                                          
      Depletion Cellular                               1 (2%)                    1 (2%)                                             
      Fibrosis, Focal                                  2 (4%)                                                                       
      Hematopoietic Cell Proliferation                 9 (18%)     15 (30%)     10 (20%)     11 (22%)                               
      Hyperplasia, Lymphoid                            3 (6%)       2 (4%)       1 (2%)       1 (2%)                                
      Inflammation, Focal                                                                     1 (2%)                                
   Thymus                                             (47)         (40)         (44)         (45)                                   
      Atrophy                                          1 (2%)                                                                       
      Cyst                                             3 (6%)       3 (8%)       1 (2%)                                             
      Hyperplasia, Lymphoid                            1 (2%)                                 1 (2%)                                
      Epithelial Cell, Hyperplasia                     1 (2%)       1 (3%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Skin                                               (50)         (50)         (50)         (50)                                   
      Subcutaneous Tissue, Edema                                                 1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 05205-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           P-NITROTOLUENE                                      Date: 02/08/00  
Route: DOSED FEED                                                                                                 Time: 09:18:07  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE MALE                          0            1250         2500         5000                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (50)         (50)         (50)         (50)                                   
      Cranium, Hyperostosis                            1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (50)         (50)         (50)         (50)                                   
      Atrophy, Focal                                                1 (2%)                                                          
      Hemorrhage, Focal                                             1 (2%)                                                          
   Peripheral Nerve                                   (1)                                                                           
      Sciatic, Degeneration                            1 (100%)                                                                     
   Spinal Cord                                        (2)                                    (1)                                    
      Meninges, Inflammation, Focal                    1 (50%)                                                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (50)         (50)         (50)                                   
      Congestion                                                    1 (2%)                    2 (4%)                                
      Embolus                                          1 (2%)                                                                       
      Hemorrhage                                                    7 (14%)      4 (8%)       1 (2%)                                
      Hyperplasia, Histiocytic                                      1 (2%)       1 (2%)       1 (2%)                                
      Hyperplasia, Lymphoid                                                                   1 (2%)                                
      Infiltration Cellular, Mixed Cell                                                       2 (4%)                                
      Thrombosis                                                                 1 (2%)                                             
      Alveolar Epithelium, Bronchiolization                        20 (40%)     30 (60%)     48 (96%)                               
      Alveolar Epithelium, Hyperplasia                 1 (2%)       1 (2%)       4 (8%)       6 (12%)                               
      Bronchiole, Hyperplasia                                                                 1 (2%)                                
      Mediastinum, Inflammation, Chronic               1 (2%)                                                                       
   Nose                                               (50)         (50)         (50)         (50)                                   
      Foreign Body                                                  1 (2%)                                                          
      Inflammation, Suppurative                        1 (2%)       1 (2%)                    1 (2%)                                
      Nasolacrimal Duct, Cyst                                       1 (2%)                                                          
      Nasolacrimal Duct, Inflammation                               1 (2%)                                                          
      Sinus, Foreign Body                              1 (2%)       2 (4%)       2 (4%)       2 (4%)                                
      Sinus, Inflammation, Chronic, Suppurative        1 (2%)       3 (6%)       3 (6%)       2 (4%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                (1)          (1)                                                              
      Cataract                                         1 (100%)                                                                     
      Cornea, Inflammation, Chronic                                 1 (100%)                                                        
      Cornea, Necrosis, Focal                          1 (100%)                                                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    

a  Number of animals examined microscopically at site and number of animals with lesion                                             
NTP Experiment-Test: 05205-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                           P-NITROTOLUENE                                      Date: 02/08/00  
Route: DOSED FEED                                                                                                 Time: 09:18:07  
____________________________________________________________________________________________________________________________________

         B6C3F1 MICE MALE                          0            1250         2500         5000                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (50)         (49)         (50)         (50)                                   
      Atrophy, Focal                                   1 (2%)                                                                       
      Congestion                                       2 (4%)       2 (4%)       2 (4%)                                             
      Cyst                                             2 (4%)                    1 (2%)                                             
      Cyst, Multiple                                   1 (2%)                                                                       
      Inflammation, Chronic                                         1 (2%)                                                          
      Nephropathy                                     46 (92%)     49 (100%)    47 (94%)     43 (86%)                               
      Nephropathy, Focal                               1 (2%)                                 1 (2%)                                
      Glomerulus, Thrombosis                                                     1 (2%)                                             
      Pelvis, Dilatation                                                         1 (2%)                                             
      Renal Tubule, Accumulation, Hyaline Droplet                                1 (2%)                                             
      Renal Tubule, Hyperplasia, Focal                 1 (2%)       1 (2%)       2 (4%)                                             
      Renal Tubule, Pigmentation                                                 1 (2%)                                             
                                                                                                                                    

a  Number of animals examined microscopically at site and number of animals with lesion                                             
                             ------------------------------------------------------------                                           
                             ----------              END OF REPORT             ----------                                           
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