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TDMS Study 05211-05 Pathology Tables


NTP Experiment-Test: 05211-05         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                                               EMODIN                                          Date: 01/25/01
Route: DOSED FEED                                                                                                 Time: 12:57:48

                                                       FINAL#3;REVISION#1




       Facility:  Southern Research Institute

       Chemical CAS #:  518-82-1

       Lock Date:  07/30/97

       Cage Range:  All

       Reasons For Removal:    25022 Accidently Killed                 25019 Moribund Sacrifice
                               25020 Natural Death                     25021 Terminal Sacrifice

       Removal Date Range:     All

       Treatment Groups:       Include All

































a  Number of animals examined microscopically at site and number of animals with lesion

                                                              Page   1


NTP Experiment-Test: 05211-05         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                                               EMODIN                                          Date: 01/25/01    
Route: DOSED FEED                                                                                                 Time: 12:57:48    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                   0            280          830          2500                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially In Study                          60           60           60           60                                     
  Early Deaths                                                                                                                      
    Moribund Sacrifice                                12            8           11           15                                     
    Natural Death                                      5            3            4            1                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                33           39           35           34                                     
                                                                                                                                    
  Animals Examined Microscopically                    50           50           50           50                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Intestine Large, Cecum                             (49)         (50)         (49)         (50)                                   
      Edema                                                                      1 (2%)                                             
      Hemorrhage                                                                 1 (2%)                                             
      Inflammation, Acute                                                        1 (2%)                                             
   Intestine Small, Duodenum                          (48)         (50)         (49)         (50)                                   
      Epithelium, Hyperplasia                                                    1 (2%)                                             
   Liver                                              (49)         (50)         (50)         (50)                                   
      Angiectasis                                                                2 (4%)       4 (8%)                                
      Basophilic Focus                                39 (80%)     44 (88%)     41 (82%)     45 (90%)                               
      Clear Cell Focus                                 2 (4%)      10 (20%)      4 (8%)      11 (22%)                               
      Eosinophilic Focus                              18 (37%)     10 (20%)     18 (36%)     27 (54%)                               
      Hematopoietic Cell Proliferation                 2 (4%)                    2 (4%)       2 (4%)                                
      Hemorrhage                                       1 (2%)                    1 (2%)                                             
      Hepatodiaphragmatic Nodule                       3 (6%)       9 (18%)      7 (14%)      7 (14%)                               
      Infiltration Cellular, Mixed Cell               15 (31%)     12 (24%)      9 (18%)     11 (22%)                               
      Mixed Cell Focus                                 4 (8%)       5 (10%)      6 (12%)      1 (2%)                                
      Necrosis, Focal                                  4 (8%)       4 (8%)       3 (6%)                                             
      Bile Duct, Hyperplasia                           1 (2%)                    4 (8%)       1 (2%)                                
      Centrilobular, Necrosis                                       1 (2%)                    1 (2%)                                
      Hepatocyte, Vacuolization Cytoplasmic            3 (6%)       2 (4%)       2 (4%)       2 (4%)                                
      Kupffer Cell, Pigmentation                       2 (4%)       2 (4%)       3 (6%)       1 (2%)                                
   Mesentery                                          (1)          (6)          (1)          (6)                                    
      Accessory Spleen                                              2 (33%)                   2 (33%)                               
      Fat, Necrosis                                    1 (100%)     4 (67%)      1 (100%)     5 (83%)                               
   Pancreas                                           (49)         (50)         (49)         (50)                                   
      Atrophy                                         13 (27%)     15 (30%)     18 (37%)     13 (26%)                               
      Metaplasia, Hepatocyte                           1 (2%)                                                                       
   Salivary Glands                                    (49)         (50)         (50)         (50)                                   
      Atrophy                                          1 (2%)                    1 (2%)       2 (4%)                                
   Stomach, Forestomach                               (49)         (50)         (50)         (50)                                   
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    2                                                              
                                                                                                                                   
NTP Experiment-Test: 05211-05         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                                               EMODIN                                          Date: 01/25/01    
Route: DOSED FEED                                                                                                 Time: 12:57:48    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                   0            280          830          2500                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
      Edema                                            1 (2%)       1 (2%)       1 (2%)                                             
      Erosion                                                                    1 (2%)                                             
      Perforation                                                                1 (2%)                                             
      Epithelium, Hyperplasia                          1 (2%)                                                                       
   Stomach, Glandular                                 (49)         (50)         (50)         (50)                                   
      Edema                                                                      1 (2%)                                             
      Erosion                                                       1 (2%)       1 (2%)       1 (2%)                                
   Tongue                                                          (1)                                                              
      Epithelium, Hyperplasia                                       1 (100%)                                                        
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (49)         (50)         (50)         (50)                                   
      Cardiomyopathy                                  22 (45%)     16 (32%)     17 (34%)     16 (32%)                               
      Inflammation, Chronic Active                                  1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (49)         (50)         (50)         (50)                                   
      Accessory Adrenal Cortical Nodule                6 (12%)      4 (8%)       7 (14%)     10 (20%)                               
      Atrophy                                                                                 2 (4%)                                
      Degeneration, Fatty                             12 (24%)      9 (18%)     14 (28%)     10 (20%)                               
      Hemorrhage                                       1 (2%)       1 (2%)                                                          
      Hyperplasia, Diffuse                             1 (2%)                                                                       
      Hyperplasia, Focal                               3 (6%)       3 (6%)       8 (16%)      7 (14%)                               
      Hypertrophy                                      1 (2%)                                                                       
      Hypertrophy, Focal                              12 (24%)      7 (14%)     12 (24%)     10 (20%)                               
      Necrosis                                                      2 (4%)                                                          
   Adrenal Medulla                                    (47)         (50)         (50)         (50)                                   
      Hyperplasia                                      2 (4%)       2 (4%)       2 (4%)       3 (6%)                                
   Islets, Pancreatic                                 (49)         (50)         (49)         (50)                                   
      Hyperplasia                                                   1 (2%)                                                          
   Pituitary Gland                                    (49)         (50)         (49)         (49)                                   
      Pars Distalis, Angiectasis                       9 (18%)     10 (20%)      6 (12%)      5 (10%)                               
      Pars Distalis, Cyst                             22 (45%)     11 (22%)     13 (27%)     16 (33%)                               
      Pars Distalis, Hyperplasia, Focal               13 (27%)     10 (20%)      3 (6%)       5 (10%)                               
      Pars Intermedia, Cyst                                         1 (2%)                                                          
   Thyroid Gland                                      (49)         (50)         (50)         (50)                                   
      Ultimobranchial Cyst                             1 (2%)                    1 (2%)                                             
      C-Cell, Hyperplasia                             21 (43%)      9 (18%)     11 (22%)     12 (24%)                               
      Follicle, Cyst                                   1 (2%)       1 (2%)       2 (4%)       2 (4%)                                
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    3                                                              
                                                                                                                                   
NTP Experiment-Test: 05211-05         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                                               EMODIN                                          Date: 01/25/01    
Route: DOSED FEED                                                                                                 Time: 12:57:48    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                   0            280          830          2500                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Clitoral Gland                                     (49)         (50)         (49)         (49)                                   
      Cyst                                             1 (2%)                    4 (8%)       1 (2%)                                
      Hyperplasia                                      3 (6%)       2 (4%)       6 (12%)      3 (6%)                                
      Inflammation, Chronic                            5 (10%)      5 (10%)      6 (12%)      5 (10%)                               
   Ovary                                              (49)         (50)         (50)         (50)                                   
      Cyst                                            10 (20%)      6 (12%)      6 (12%)      7 (14%)                               
   Uterus                                             (49)         (50)         (50)         (50)                                   
      Angiectasis                                                   1 (2%)                                                          
      Hydrometra                                       5 (10%)      3 (6%)       5 (10%)      6 (12%)                               
      Hyperplasia, Cystic                              1 (2%)       5 (10%)      4 (8%)       7 (14%)                               
      Inflammation, Chronic                            1 (2%)                    1 (2%)       2 (4%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (49)         (50)         (50)         (50)                                   
      Hyperplasia                                      5 (10%)      3 (6%)       4 (8%)       6 (12%)                               
      Infiltration Cellular, Histiocyte                             2 (4%)       4 (8%)       2 (4%)                                
   Lymph Node                                         (5)          (3)          (4)          (3)                                    
      Mediastinal, Hemorrhage                                                    2 (50%)      1 (33%)                               
      Mediastinal, Hyperplasia, Lymphoid                                         1 (25%)      1 (33%)                               
      Mediastinal, Pigmentation                        1 (20%)      1 (33%)      1 (25%)                                            
      Pancreatic, Hematopoietic Cell Proliferation                               1 (25%)                                            
      Pancreatic, Hemorrhage                                                     1 (25%)                                            
   Lymph Node, Mandibular                             (49)         (50)         (50)         (50)                                   
      Ectasia                                          1 (2%)       2 (4%)       6 (12%)      6 (12%)                               
      Hemorrhage                                       2 (4%)       4 (8%)       4 (8%)       4 (8%)                                
      Hyperplasia, Lymphoid                            1 (2%)                    2 (4%)       1 (2%)                                
      Pigmentation                                    18 (37%)     19 (38%)     21 (42%)     25 (50%)                               
   Lymph Node, Mesenteric                             (49)         (50)         (49)         (50)                                   
      Hemorrhage                                       2 (4%)       5 (10%)      2 (4%)       4 (8%)                                
      Pigmentation                                     2 (4%)       2 (4%)                    2 (4%)                                
   Spleen                                             (50)         (50)         (50)         (50)                                   
      Angiectasis                                                                             1 (2%)                                
      Depletion Cellular                                            1 (2%)                                                          
      Developmental Malformation                                                              1 (2%)                                
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    4                                                              
                                                                                                                                   
NTP Experiment-Test: 05211-05         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                                               EMODIN                                          Date: 01/25/01    
Route: DOSED FEED                                                                                                 Time: 12:57:48    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                   0            280          830          2500                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - CONT                                                                                                         
      Fibrosis                                                                                2 (4%)                                
      Hematopoietic Cell Proliferation                27 (54%)     24 (48%)     31 (62%)     40 (80%)                               
      Hemorrhage                                       1 (2%)                    1 (2%)                                             
      Hyperplasia, Histiocytic                                                   1 (2%)                                             
      Necrosis                                         1 (2%)       1 (2%)                                                          
      Pigmentation                                    30 (60%)     30 (60%)     35 (70%)     43 (86%)                               
   Thymus                                             (47)         (49)         (49)         (50)                                   
      Cyst                                                                                    1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (49)         (50)         (50)         (50)                                   
      Hyperplasia                                     44 (90%)     44 (88%)     42 (84%)     42 (84%)                               
   Skin                                               (49)         (50)         (50)         (50)                                   
      Hyperkeratosis                                   1 (2%)       1 (2%)       1 (2%)       3 (6%)                                
      Inflammation, Chronic                            1 (2%)                    1 (2%)                                             
      Ulcer                                            2 (4%)       1 (2%)       4 (8%)       3 (6%)                                
      Epidermis, Hyperplasia                           4 (8%)       2 (4%)       5 (10%)      5 (10%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (49)         (50)         (50)         (50)                                   
      Cranium, Osteopetrosis                           7 (14%)      5 (10%)      6 (12%)      8 (16%)                               
      Femur, Osteopetrosis                             6 (12%)      8 (16%)      7 (14%)      7 (14%)                               
   Skeletal Muscle                                                              (1)                                                 
      Atrophy                                                                    1 (100%)                                           
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (49)         (50)         (50)         (50)                                   
      Compression                                      8 (16%)      7 (14%)     10 (20%)      7 (14%)                               
      Hydrocephalus                                    3 (6%)                    3 (6%)       7 (14%)                               
   Peripheral Nerve                                   (2)          (1)          (3)                                                 
      Atrophy                                                                    1 (33%)                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (49)         (50)         (50)         (50)                                   
      Hemorrhage                                       3 (6%)       1 (2%)       2 (4%)       5 (10%)                               
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    5                                                              
                                                                                                                                   
NTP Experiment-Test: 05211-05         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                                               EMODIN                                          Date: 01/25/01    
Route: DOSED FEED                                                                                                 Time: 12:57:48    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                   0            280          830          2500                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
RESPIRATORY SYSTEM - CONT                                                                                                           
      Infiltration Cellular, Histiocyte               38 (78%)     37 (74%)     34 (68%)     32 (64%)                               
      Inflammation, Chronic                            1 (2%)       1 (2%)       1 (2%)                                             
      Alveolar Epithelium, Hyperplasia                 3 (6%)       4 (8%)                    4 (8%)                                
   Nose                                               (49)         (50)         (50)         (50)                                   
      Foreign Body                                     1 (2%)                    1 (2%)       2 (4%)                                
      Inflammation, Chronic                            6 (12%)      3 (6%)       5 (10%)      5 (10%)                               
      Goblet Cell, Hyperplasia                         1 (2%)                    1 (2%)                                             
      Respiratory Epithelium, Hyperplasia              6 (12%)      4 (8%)       4 (8%)       1 (2%)                                
      Respiratory Epithelium, Metaplasia, Squamous     1 (2%)       1 (2%)                    2 (4%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                (5)          (2)          (4)          (2)                                    
      Cataract                                         4 (80%)      2 (100%)     3 (75%)      1 (50%)                               
      Hemorrhage                                                                 2 (50%)                                            
      Inflammation, Chronic                                                      1 (25%)      1 (50%)                               
      Retina, Degeneration                             4 (80%)      2 (100%)     3 (75%)      1 (50%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (49)         (50)         (49)         (50)                                   
      Cyst                                                                       1 (2%)                                             
      Glomerulosclerosis                                                         1 (2%)                                             
      Hydronephrosis                                                             1 (2%)                                             
      Infarct                                                                    2 (4%)       3 (6%)                                
      Inflammation, Chronic                                         1 (2%)       1 (2%)       3 (6%)                                
      Nephropathy                                     46 (94%)     43 (86%)     45 (92%)     49 (98%)                               
      Papilla, Necrosis                                                          1 (2%)                                             
      Renal Tubule, Hyaline Droplet                   22 (45%)     49 (98%)     49 (100%)    50 (100%)                              
      Renal Tubule, Hyperplasia, Oncocytic                                                    2 (4%)                                
      Renal Tubule, Necrosis                           1 (2%)                                                                       
      Renal Tubule, Pigmentation                      45 (92%)     49 (98%)     49 (100%)    50 (100%)                              
      Transitional Epithelium, Hyperplasia             2 (4%)                    1 (2%)       1 (2%)                                
   Urinary Bladder                                    (49)         (50)         (50)         (50)                                   
      Hemorrhage                                                                 1 (2%)                                             
      Necrosis                                                                   1 (2%)                                             
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page    6                                                              
                                                                                                                                   
NTP Experiment-Test: 05211-05         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                                               EMODIN                                          Date: 01/25/01    
Route: DOSED FEED                                                                                                 Time: 12:57:48    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS MALE                     0            280          830          2500                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially In Study                          60           60           60           60                                     
  Early Deaths                                                                                                                      
    Moribund Sacrifice                                17           26           27           16                                     
    Natural Death                                      3            3            2            4                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                30           21           21           30                                     
                                                                                                                                    
  Animals Examined Microscopically                    50           50           50           50                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Intestine Large, Colon                             (49)         (50)         (50)         (50)                                   
      Congestion                                                    1 (2%)                                                          
      Edema                                                         1 (2%)                                                          
      Inflammation, Chronic                                                                   1 (2%)                                
      Epithelium, Hyperplasia                                                                 1 (2%)                                
   Intestine Large, Cecum                             (49)         (50)         (50)         (49)                                   
      Congestion                                                    1 (2%)                                                          
      Edema                                            2 (4%)       2 (4%)       3 (6%)       2 (4%)                                
      Inflammation, Chronic                                                                   2 (4%)                                
      Epithelium, Hyperplasia                                                                 2 (4%)                                
   Intestine Small, Jejunum                           (49)         (48)         (50)         (49)                                   
      Congestion                                                    1 (2%)                                                          
      Epithelium, Hyperplasia                                                                 1 (2%)                                
   Liver                                              (50)         (50)         (50)         (50)                                   
      Angiectasis                                      5 (10%)      5 (10%)                  11 (22%)                               
      Basophilic Focus                                20 (40%)     18 (36%)     18 (36%)     23 (46%)                               
      Clear Cell Focus                                 7 (14%)      7 (14%)      9 (18%)     13 (26%)                               
      Degeneration, Cystic                             7 (14%)     10 (20%)     11 (22%)     10 (20%)                               
      Eosinophilic Focus                               6 (12%)      8 (16%)      6 (12%)     10 (20%)                               
      Hematopoietic Cell Proliferation                 2 (4%)                                                                       
      Hemorrhage                                                                 1 (2%)                                             
      Hepatodiaphragmatic Nodule                       3 (6%)       5 (10%)      2 (4%)       4 (8%)                                
      Infiltration Cellular, Mixed Cell                             2 (4%)       2 (4%)       2 (4%)                                
      Mixed Cell Focus                                 6 (12%)      4 (8%)       3 (6%)       9 (18%)                               
      Necrosis, Focal                                  2 (4%)       2 (4%)       2 (4%)                                             
      Bile Duct, Hyperplasia                          33 (66%)     24 (48%)     20 (40%)     14 (28%)                               
      Centrilobular, Necrosis                          1 (2%)       1 (2%)                                                          
      Hepatocyte, Vacuolization Cytoplasmic            3 (6%)       3 (6%)       4 (8%)       2 (4%)                                
      Kupffer Cell, Pigmentation                       1 (2%)       1 (2%)       2 (4%)       4 (8%)                                
   Mesentery                                          (8)          (7)          (13)         (15)                                   
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    7                                                              
                                                                                                                                   
NTP Experiment-Test: 05211-05         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                                               EMODIN                                          Date: 01/25/01    
Route: DOSED FEED                                                                                                 Time: 12:57:48    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS MALE                     0            280          830          2500                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
      Accessory Spleen                                 4 (50%)      2 (29%)      2 (15%)                                            
      Fat, Necrosis                                    5 (63%)      6 (86%)     11 (85%)     12 (80%)                               
   Pancreas                                           (50)         (50)         (50)         (50)                                   
      Atrophy                                         23 (46%)     19 (38%)     16 (32%)     23 (46%)                               
      Metaplasia, Hepatocyte                           1 (2%)                    1 (2%)                                             
      Acinus, Hyperplasia, Focal                       1 (2%)       1 (2%)                    1 (2%)                                
   Salivary Glands                                    (50)         (50)         (50)         (49)                                   
      Edema                                                         1 (2%)                                                          
   Stomach, Forestomach                               (50)         (50)         (49)         (48)                                   
      Diverticulum                                     1 (2%)                                                                       
      Edema                                            4 (8%)       3 (6%)       2 (4%)       5 (10%)                               
      Perforation                                                                1 (2%)                                             
      Ulcer                                            6 (12%)      2 (4%)       4 (8%)       4 (8%)                                
      Epithelium, Hyperplasia                          5 (10%)      4 (8%)       7 (14%)      5 (10%)                               
   Stomach, Glandular                                 (50)         (50)         (49)         (48)                                   
      Edema                                            1 (2%)       3 (6%)                                                          
      Erosion                                          1 (2%)       2 (4%)       3 (6%)       1 (2%)                                
      Ulcer                                            2 (4%)       3 (6%)       1 (2%)       1 (2%)                                
      Epithelium, Hyperplasia                                       1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (50)         (50)         (50)         (50)                                   
      Cardiomyopathy                                  34 (68%)     31 (62%)     35 (70%)     36 (72%)                               
      Thrombosis                                                    2 (4%)       4 (8%)       2 (4%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (50)         (50)         (50)         (50)                                   
      Accessory Adrenal Cortical Nodule               13 (26%)     18 (36%)     16 (32%)     10 (20%)                               
      Degeneration, Fatty                              7 (14%)      9 (18%)     10 (20%)      8 (16%)                               
      Hyperplasia, Diffuse                                          2 (4%)       1 (2%)       1 (2%)                                
      Hyperplasia, Focal                               3 (6%)                    4 (8%)       2 (4%)                                
      Hypertrophy                                                                1 (2%)                                             
      Hypertrophy, Focal                               6 (12%)      9 (18%)      7 (14%)      3 (6%)                                
      Necrosis                                                      1 (2%)                                                          
   Adrenal Medulla                                    (50)         (49)         (50)         (50)                                   
      Hyperplasia                                     10 (20%)      9 (18%)      7 (14%)     17 (34%)                               
   Islets, Pancreatic                                 (50)         (50)         (49)         (50)                                   
      Hyperplasia                                      5 (10%)                   1 (2%)       2 (4%)                                
   Parathyroid Gland                                  (48)         (45)         (45)         (47)                                   
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    8                                                              
                                                                                                                                   
NTP Experiment-Test: 05211-05         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                                               EMODIN                                          Date: 01/25/01    
Route: DOSED FEED                                                                                                 Time: 12:57:48    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS MALE                     0            280          830          2500                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ENDOCRINE SYSTEM - CONT                                                                                                             
      Hyperplasia, Focal                                            1 (2%)                                                          
   Pituitary Gland                                    (49)         (50)         (48)         (50)                                   
      Pars Distalis, Angiectasis                       1 (2%)                    1 (2%)       1 (2%)                                
      Pars Distalis, Cyst                              7 (14%)      9 (18%)      5 (10%)      3 (6%)                                
      Pars Distalis, Hyperplasia, Focal                6 (12%)      3 (6%)       5 (10%)      1 (2%)                                
      Pars Intermedia, Cyst                                         1 (2%)                                                          
   Thyroid Gland                                      (50)         (50)         (50)         (50)                                   
      Ultimobranchial Cyst                                                       1 (2%)       1 (2%)                                
      C-Cell, Hyperplasia                              7 (14%)      2 (4%)       7 (14%)      4 (8%)                                
      Follicle, Cyst                                   1 (2%)       3 (6%)       1 (2%)       1 (2%)                                
      Follicular Cell, Hyperplasia                     1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Epididymis                                         (50)         (50)         (50)         (50)                                   
      Atypia Cellular                                 29 (58%)     26 (52%)     25 (50%)     29 (58%)                               
   Preputial Gland                                    (50)         (50)         (50)         (50)                                   
      Cyst                                             1 (2%)                    1 (2%)                                             
      Inflammation, Chronic                           17 (34%)     20 (40%)     13 (26%)     20 (40%)                               
   Prostate                                           (50)         (50)         (50)         (50)                                   
      Fibrosis                                         5 (10%)      5 (10%)      4 (8%)       6 (12%)                               
      Inflammation, Chronic                           27 (54%)     24 (48%)     28 (56%)     29 (58%)                               
      Epithelium, Hyperplasia                         12 (24%)      3 (6%)      10 (20%)      9 (18%)                               
   Testes                                             (50)         (50)         (50)         (50)                                   
      Germinal Epithelium, Atrophy                     7 (14%)     12 (24%)      3 (6%)       8 (16%)                               
      Interstitial Cell, Hyperplasia                   4 (8%)       8 (16%)      3 (6%)       5 (10%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (50)         (50)         (50)         (50)                                   
      Hyperplasia                                      3 (6%)       5 (10%)      3 (6%)      11 (22%)                               
   Lymph Node                                         (12)         (21)         (18)         (11)                                   
      Iliac, Pigmentation                                           1 (5%)                    1 (9%)                                
      Mediastinal, Hemorrhage                          1 (8%)       1 (5%)       2 (11%)      4 (36%)                               
      Mediastinal, Hyperplasia, Lymphoid               1 (8%)                    1 (6%)                                             
      Mediastinal, Pigmentation                                     2 (10%)      4 (22%)      2 (18%)                               
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    9                                                              
                                                                                                                                   
NTP Experiment-Test: 05211-05         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                                               EMODIN                                          Date: 01/25/01    
Route: DOSED FEED                                                                                                 Time: 12:57:48    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS MALE                     0            280          830          2500                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - CONT                                                                                                         
      Pancreatic, Hemorrhage                                                                  1 (9%)                                
      Pancreatic, Pigmentation                                      1 (5%)                                                          
      Renal, Hemorrhage                                                          1 (6%)                                             
      Renal, Pigmentation                                                        1 (6%)       1 (9%)                                
   Lymph Node, Mandibular                             (49)         (50)         (48)         (50)                                   
      Ectasia                                                       3 (6%)       2 (4%)       1 (2%)                                
      Hemorrhage                                                                 1 (2%)       2 (4%)                                
      Hyperplasia, Lymphoid                            1 (2%)                    1 (2%)                                             
      Pigmentation                                                  2 (4%)                    2 (4%)                                
   Lymph Node, Mesenteric                             (50)         (50)         (50)         (50)                                   
      Atrophy                                                       1 (2%)                                                          
      Ectasia                                                                                 6 (12%)                               
      Hemorrhage                                       2 (4%)       2 (4%)       2 (4%)       2 (4%)                                
      Hyperplasia, Lymphoid                                                                   1 (2%)                                
   Spleen                                             (50)         (50)         (50)         (50)                                   
      Accessory Spleen                                 1 (2%)                    1 (2%)                                             
      Fibrosis                                         8 (16%)      9 (18%)      3 (6%)       3 (6%)                                
      Hematopoietic Cell Proliferation                 3 (6%)       5 (10%)      4 (8%)       8 (16%)                               
      Necrosis                                         1 (2%)       1 (2%)       2 (4%)       1 (2%)                                
      Pigmentation                                     1 (2%)       2 (4%)       4 (8%)       4 (8%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (49)         (47)         (49)         (49)                                   
      Hyperplasia                                     14 (29%)     23 (49%)     10 (20%)     13 (27%)                               
   Skin                                               (50)         (50)         (49)         (49)                                   
      Cyst Epithelial Inclusion                                                  3 (6%)       2 (4%)                                
      Hyperkeratosis                                   2 (4%)       3 (6%)       1 (2%)                                             
      Hyperplasia, Basal Cell                                       1 (2%)                                                          
      Inflammation, Chronic                            2 (4%)       2 (4%)                    1 (2%)                                
      Ulcer                                                         2 (4%)                                                          
      Epidermis, Hyperplasia                           1 (2%)       3 (6%)                                                          
      Subcutaneous Tissue, Fibrosis                                 1 (2%)                                                          
      Subcutaneous Tissue, Inflammation, Chronic                                                                                    
          Active                                                                              1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (50)         (50)         (50)         (50)                                   
      Cranium, Osteopetrosis                           1 (2%)                                                                       
   Skeletal Muscle                                    (1)                                    (2)                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page   10                                                              
                                                                                                                                   
NTP Experiment-Test: 05211-05         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                                               EMODIN                                          Date: 01/25/01    
Route: DOSED FEED                                                                                                 Time: 12:57:48    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS MALE                     0            280          830          2500                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
MUSCULOSKELETAL SYSTEM - CONT                                                                                                       
      Hemorrhage                                                                              1 (50%)                               
      Necrosis                                                                                1 (50%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (50)         (50)         (50)         (50)                                   
      Angiectasis                                                                             1 (2%)                                
      Compression                                      4 (8%)       2 (4%)       5 (10%)      5 (10%)                               
      Hydrocephalus                                    1 (2%)       1 (2%)       2 (4%)       1 (2%)                                
      Necrosis                                         1 (2%)       1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (50)         (50)         (50)                                   
      Hemorrhage                                                    1 (2%)       2 (4%)       1 (2%)                                
      Infiltration Cellular, Histiocyte               26 (52%)     28 (56%)     27 (54%)     35 (70%)                               
      Inflammation, Chronic                            1 (2%)                                 2 (4%)                                
      Alveolar Epithelium, Hyperplasia                 5 (10%)      1 (2%)       2 (4%)       5 (10%)                               
   Nose                                               (50)         (50)         (50)         (50)                                   
      Foreign Body                                     4 (8%)       3 (6%)       1 (2%)       2 (4%)                                
      Inflammation, Chronic                            9 (18%)      8 (16%)      9 (18%)      8 (16%)                               
      Respiratory Epithelium, Hyperplasia              6 (12%)      7 (14%)      8 (16%)      5 (10%)                               
      Respiratory Epithelium, Metaplasia, Squamous     4 (8%)       3 (6%)       5 (10%)      1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                (3)          (1)          (1)                                                 
      Atrophy                                          1 (33%)                                                                      
      Cataract                                         2 (67%)      1 (100%)     1 (100%)                                           
      Retina, Degeneration                             2 (67%)      1 (100%)     1 (100%)                                           
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (50)         (50)         (50)         (50)                                   
      Cyst                                                          1 (2%)       2 (4%)       1 (2%)                                
      Hydronephrosis                                                                          2 (4%)                                
      Infarct                                                                                 3 (6%)                                
      Inflammation, Chronic                           15 (30%)     11 (22%)     13 (26%)     25 (50%)                               
      Nephropathy                                     48 (96%)     50 (100%)    50 (100%)    50 (100%)                              
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page   11                                                              
                                                                                                                                   
NTP Experiment-Test: 05211-05         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: CHRONIC                                               EMODIN                                          Date: 01/25/01    
Route: DOSED FEED                                                                                                 Time: 12:57:48    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS MALE                     0            280          830          2500                                      
                                                   PPM          PPM          PPM          PPM                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
URINARY SYSTEM - CONT                                                                                                               
      Renal Tubule, Hyaline Droplet                    3 (6%)      45 (90%)     43 (86%)     43 (86%)                               
      Renal Tubule, Hyperplasia                                                  1 (2%)                                             
      Renal Tubule, Necrosis                                        1 (2%)                    1 (2%)                                
      Renal Tubule, Pigmentation                      35 (70%)     47 (94%)     49 (98%)     50 (100%)                              
      Transitional Epithelium, Hyperplasia             4 (8%)       4 (8%)       3 (6%)       2 (4%)                                
   Urinary Bladder                                    (50)         (50)         (50)         (50)                                   
      Edema                                                                                   1 (2%)                                
      Hemorrhage                                                                              1 (2%)                                
      Transitional Epithelium, Hyperplasia                                       1 (2%)                                             
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   12                                                              
                                                                                                                                   
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                             ----------              END OF REPORT             ----------                                           
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