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https://ntp.niehs.nih.gov/go/4613

TDMS Study 05706-01 Pathology Tables



                                     INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)
                                              4,4-DIAMINO-2,2-STILBENEDISULFONIC ACID


NTP Experiment-Test: 05706-01                                                                                     Report: PEIRPT03
Study Type: CHRONIC                                                                                               Date: 09/18/94
Route: DOSED FEED                                                                                                 Time: 22:48:35




       Facility:  International Research and Development Corp.

       Chemical CAS #:  81-11-8

       Lock Date:  None

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All
































a  Number of animals examined microscopically at site and number of animals with lesion

                                                             Page   1

NTP Experiment-Test: 05706-01         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                              4,4-DIAMINO-2,2-STILBENEDISULFONIC ACID                          Date: 09/18/94  
Route: DOSED FEED                                                                                                 Time: 22:48:35  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                        CONTROL      6250PPM      12500PPM                                               
                                                   FEMALE       FEMALE       FEMALE                                                 
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY                                                                                                                 
  Animals Initially In Study                          60           60           60                                                  
  Scheduled Sacrifice                                 10           10           10                                                  
  Early Deaths                                                                                                                      
    Dead                                               5            5            6                                                  
    Moribund                                           2            2            5                                                  
  Survivors                                                                                                                         
    Terminal Sacrifice                                43           43           38                                                  
  Missing                                                                        1                                                  
  Animals Examined Microscopically                    50           50           49                                                  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Gallbladder                                        (45)         (3)          (43)                                                
      Infiltration Cellular, Lymphocyte                1 (2%)                                                                       
   Intestine Large, Cecum                             (48)         (4)          (45)                                                
      Peyer's Patch, Hyperplasia, Lymphoid             5 (10%)                   5 (11%)                                            
   Intestine Small, Jejunum                           (49)         (28)         (43)                                                
      Hemorrhage                                                                 1 (2%)                                             
      Peyer's Patch, Angiectasis                                    1 (4%)                                                          
      Peyer's Patch, Hyperplasia, Lymphoid             5 (10%)      3 (11%)      4 (9%)                                             
   Liver                                              (50)         (17)         (49)                                                
      Basophilic Focus, Multiple                       1 (2%)                                                                       
      Cytoplasmic Alteration, Focal                                 1 (6%)       1 (2%)                                             
      Fatty Change, Diffuse                            1 (2%)       1 (6%)       2 (4%)                                             
      Fatty Change, Focal                              1 (2%)                                                                       
      Granuloma                                        1 (2%)       1 (6%)                                                          
      Hematocyst                                                                 1 (2%)                                             
      Hematopoietic Cell Proliferation                 1 (2%)       1 (6%)       1 (2%)                                             
      Infarct                                                       1 (6%)                                                          
      Infiltration Cellular, Lymphocyte               10 (20%)      2 (12%)      4 (8%)                                             
      Mitotic Alteration                               1 (2%)                                                                       
      Necrosis, Focal                                  3 (6%)       2 (12%)      4 (8%)                                             
      Pigmentation, Lipofuscin                                      1 (6%)                                                          
      Bile Duct, Hyperplasia, Multifocal               1 (2%)                                                                       
      Centrilobular, Fatty Change, Diffuse             1 (2%)                                                                       
      Periportal, Fatty Change, Diffuse                2 (4%)                                                                       
   Mesentery                                          (5)          (1)          (1)                                                 
      Amyloid Deposition                               1 (20%)                                                                      
      Fat, Necrosis, Focal                             2 (40%)      1 (100%)                                                        
   Pancreas                                           (50)         (6)          (48)                                                
      Amyloid Deposition                                            1 (17%)                                                         
      Infiltration Cellular, Multifocal, Lymphocyte   12 (24%)                   8 (17%)                                            
      Acinus, Atrophy                                               1 (17%)      1 (2%)                                             
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    2                                                              
NTP Experiment-Test: 05706-01         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                              4,4-DIAMINO-2,2-STILBENEDISULFONIC ACID                          Date: 09/18/94  
Route: DOSED FEED                                                                                                 Time: 22:48:35  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                        CONTROL      6250PPM      12500PPM                                               
                                                   FEMALE       FEMALE       FEMALE                                                 
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
      Artery, Inflammation, Chronic                    1 (2%)                                                                       
      Duct, Cyst                                                    1 (17%)                                                         
   Salivary Glands                                    (50)         (6)          (47)                                                
      Infiltration Cellular, Multifocal, Lymphocyte   21 (42%)      1 (17%)     20 (43%)                                            
   Stomach, Forestomach                               (49)         (7)          (49)                                                
      Diverticulum                                                  1 (14%)                                                         
      Epithelium, Hyperplasia, Focal                   2 (4%)                                                                       
   Stomach, Glandular                                 (50)         (6)          (48)                                                
      Hemorrhage                                                    1 (17%)                                                         
      Infiltration Cellular, Lymphocyte                                          1 (2%)                                             
      Mineralization, Focal                            1 (2%)                                                                       
      Necrosis, Multifocal                                                       1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Blood Vessel                                       (1)                                                                           
      Artery, Inflammation, Chronic Active             1 (100%)                                                                     
   Heart                                              (50)         (6)          (49)                                                
      Bacterium                                                                  1 (2%)                                             
      Infiltration Cellular, Lymphocyte                                          1 (2%)                                             
      Aortic Valve, Thrombosis                                                   1 (2%)                                             
      Arteriole, Thrombosis, Multifocal                1 (2%)                                                                       
      Artery, Hypertrophy, Multifocal                                            1 (2%)                                             
      Mitral Valve, Inflammation, Acute                                          1 (2%)                                             
      Myocardium, Degeneration                         1 (2%)                                                                       
      Ventricle, Thrombosis                            1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Gland                                      (50)         (7)          (48)                                                
      Capsule, Ectopic Tissue                          4 (8%)                    1 (2%)                                             
      Capsule, Hyperplasia, Multifocal                50 (100%)     5 (71%)     48 (100%)                                           
   Adrenal Gland, Cortex                              (50)         (6)          (48)                                                
      Atrophy                                          1 (2%)                                                                       
      Degeneration, Fatty, Multifocal                  1 (2%)                                                                       
      Hematopoietic Cell Proliferation                 2 (4%)       1 (17%)                                                         
      Hemorrhage                                                                 1 (2%)                                             
      Hyperplasia, Focal                               3 (6%)                    3 (6%)                                             
      Hypertrophy, Focal                               1 (2%)                    1 (2%)                                             
   Adrenal Gland, Medulla                             (49)         (6)          (47)                                                
      Hyperplasia, Focal                               1 (2%)                                                                       
   Islets, Pancreatic                                 (50)         (5)          (48)                                                
      Hyperplasia, Multifocal                          2 (4%)                    1 (2%)                                             
   Parathyroid Gland                                  (35)                      (32)                                                
      Hyperplasia                                                                1 (3%)                                             
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    3                                                              
NTP Experiment-Test: 05706-01         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                              4,4-DIAMINO-2,2-STILBENEDISULFONIC ACID                          Date: 09/18/94  
Route: DOSED FEED                                                                                                 Time: 22:48:35  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                        CONTROL      6250PPM      12500PPM                                               
                                                   FEMALE       FEMALE       FEMALE                                                 
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ENDOCRINE SYSTEM - CONT                                                                                                             
      Infiltration Cellular, Lymphocyte                1 (3%)                                                                       
   Pituitary Gland                                    (50)         (7)          (48)                                                
      Pars Distalis, Angiectasis                       3 (6%)                    1 (2%)                                             
      Pars Distalis, Hyperplasia, Focal                1 (2%)                    3 (6%)                                             
      Pars Distalis, Hypertrophy, Focal                                          1 (2%)                                             
   Thyroid Gland                                      (50)         (7)          (46)                                                
      Infiltration Cellular, Lymphocyte                2 (4%)                    1 (2%)                                             
      Inflammation, Acute                                                        1 (2%)                                             
      Follicle, Cyst, Multiple                                                   1 (2%)                                             
      Follicular Cell, Hyperplasia, Focal              3 (6%)                    2 (4%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Ovary                                              (50)         (15)         (49)                                                
      Abscess                                                                    1 (2%)                                             
      Cyst                                            16 (32%)      7 (47%)     20 (41%)                                            
      Hemorrhage                                                                 1 (2%)                                             
      Infiltration Cellular, Lymphocyte                3 (6%)                    1 (2%)                                             
      Pigmentation, Hemosiderin                                     1 (7%)                                                          
   Uterus                                             (50)         (45)         (48)                                                
      Angiectasis                                      2 (4%)                                                                       
      Dilatation                                       7 (14%)      5 (11%)      3 (6%)                                             
      Hyperplasia, Cystic                             44 (88%)     41 (91%)     39 (81%)                                            
      Infiltration Cellular, Lymphocyte                1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (50)         (7)          (48)                                                
      Atrophy, Focal                                   2 (4%)                    2 (4%)                                             
      Hyperplasia, Neutrophil                                       1 (14%)      2 (4%)                                             
   Lymph Node                                         (48)         (17)         (46)                                                
      Hyperplasia, Lymphoid                            1 (2%)                                                                       
      Iliac, Hemorrhage                                                          1 (2%)                                             
      Iliac, Hyperplasia, Histiocytic                                            1 (2%)                                             
      Iliac, Hyperplasia, Lymphoid                     1 (2%)       2 (12%)      1 (2%)                                             
      Inguinal, Hyperplasia, Lymphoid                  1 (2%)                    1 (2%)                                             
      Mandibular, Hyperplasia, Lymphoid                2 (4%)       1 (6%)       2 (4%)                                             
      Mandibular, Pigmentation, Hemosiderin            1 (2%)                                                                       
      Mediastinal, Hyperplasia, Lymphoid               1 (2%)       1 (6%)       2 (4%)                                             
      Mediastinal, Mineralization                                   1 (6%)                                                          
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    4                                                              
NTP Experiment-Test: 05706-01         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                              4,4-DIAMINO-2,2-STILBENEDISULFONIC ACID                          Date: 09/18/94  
Route: DOSED FEED                                                                                                 Time: 22:48:35  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                        CONTROL      6250PPM      12500PPM                                               
                                                   FEMALE       FEMALE       FEMALE                                                 
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - CONT                                                                                                         
      Renal, Hemorrhage                                                          1 (2%)                                             
      Renal, Hyperplasia, Lymphoid                                  1 (6%)       1 (2%)                                             
   Lymph Node, Mesenteric                             (48)         (5)          (46)                                                
      Abscess                                                                    1 (2%)                                             
      Amyloid Deposition                               1 (2%)                                                                       
      Hemorrhage                                                    2 (40%)      1 (2%)                                             
      Hyperplasia, Lymphoid                            5 (10%)                   1 (2%)                                             
   Spleen                                             (50)         (29)         (49)                                                
      Ectopic Tissue                                                1 (3%)                                                          
      Hematopoietic Cell Proliferation                 4 (8%)       3 (10%)      4 (8%)                                             
      Hyperplasia, Lymphoid                            4 (8%)       1 (3%)       1 (2%)                                             
      Capsule, Inflammation, Chronic                                             1 (2%)                                             
   Thymus                                             (46)         (7)          (46)                                                
      Amyloid Deposition                               1 (2%)                                                                       
      Depletion Lymphoid                               4 (9%)       3 (43%)      4 (9%)                                             
      Ectopic Parathyroid Gland                                                  2 (4%)                                             
      Hyperplasia, Lymphoid                            4 (9%)                    6 (13%)                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (45)         (6)          (43)                                                
      Hyperplasia                                      2 (4%)                    2 (5%)                                             
      Inflammation, Chronic                                         1 (17%)                                                         
   Skin                                               (50)         (27)         (49)                                                
      Inflammation, Acute                                                        1 (2%)                                             
      Inflammation, Chronic                           21 (42%)     13 (48%)     13 (27%)                                            
      Ulcer                                            1 (2%)                    1 (2%)                                             
      Epithelium, Hyperplasia                          2 (4%)       2 (7%)                                                          
      Epithelium, Mitotic Alteration                                1 (4%)                                                          
      Subcutaneous Tissue, Inflammation, Acute         1 (2%)                                                                       
      Subcutaneous Tissue, Mineralization, Focal                    1 (4%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (50)         (7)          (49)                                                
      Fibrous Osteodystrophy                           6 (12%)      1 (14%)      9 (18%)                                            
      Hyperplasia                                      1 (2%)                                                                       
      Coccygeal, Fracture                                                        1 (2%)                                             
   Skeletal Muscle                                                              (2)                                                 
      Infiltration Cellular, Lymphocyte                                          1 (50%)                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    5                                                              
NTP Experiment-Test: 05706-01         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                              4,4-DIAMINO-2,2-STILBENEDISULFONIC ACID                          Date: 09/18/94  
Route: DOSED FEED                                                                                                 Time: 22:48:35  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                        CONTROL      6250PPM      12500PPM                                               
                                                   FEMALE       FEMALE       FEMALE                                                 
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (50)         (6)          (49)                                                
      Hemorrhage                                                    1 (17%)                                                         
      Mineralization, Multifocal                      34 (68%)      1 (17%)     31 (63%)                                            
      Arteriole, Meninges, Bacterium                                             1 (2%)                                             
      Arteriole, Meninges, Thrombosis                                            1 (2%)                                             
      Meninges, Inflammation, Acute                                              1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (49)         (49)                                                
      Atelectasis, Focal                               1 (2%)                                                                       
      Hemorrhage, Focal                                1 (2%)                    2 (4%)                                             
      Infiltration Cellular, Lymphocyte                1 (2%)                                                                       
      Alveolar Epithelium, Hyperplasia, Focal          3 (6%)       5 (10%)                                                         
      Alveolus, Edema                                                            1 (2%)                                             
      Alveolus, Infiltration Cellular, Histiocyte      3 (6%)                    1 (2%)                                             
      Artery, Hypertrophy, Multifocal                 10 (20%)      8 (16%)     21 (43%)                                            
      Artery, Mineralization, Multifocal                            1 (2%)                                                          
      Artery, Thrombosis                               1 (2%)       1 (2%)                                                          
      Interstitium, Inflammation, Acute                                          1 (2%)                                             
      Mediastinum, Infiltration Cellular,                                                                                           
          Lymphocyte                                                             1 (2%)                                             
      Peribronchial, Infiltration Cellular,                                                                                         
          Lymphocyte                                   1 (2%)       2 (4%)                                                          
      Peribronchiolar, Infiltration Cellular,                                                                                       
          Lymphocyte                                   4 (8%)       1 (2%)       3 (6%)                                             
      Perivascular, Infiltration Cellular,                                                                                          
          Lymphocyte                                  16 (32%)     13 (27%)     22 (45%)                                            
      Pleura, Infiltration Cellular, Lymphocyte        1 (2%)       1 (2%)       1 (2%)                                             
   Nose                                               (50)         (6)          (48)                                                
      Lumen, Foreign Body                              4 (8%)                                                                       
      Mucosa, Degeneration, Hyaline                   28 (56%)      4 (67%)     27 (56%)                                            
      Mucosa, Inflammation, Chronic                    2 (4%)                                                                       
      Mucosa, Inflammation, Chronic Active             1 (2%)                                                                       
      Nasolacrimal Duct, Inflammation, Chronic                                   1 (2%)                                             
      Nasolacrimal Duct, Inflammation, Chronic                                                                                      
          Active                                       1 (2%)                                                                       
      Respiratory Epithelium, Hyperplasia             11 (22%)      1 (17%)      9 (19%)                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    6                                                              
NTP Experiment-Test: 05706-01         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                              4,4-DIAMINO-2,2-STILBENEDISULFONIC ACID                          Date: 09/18/94  
Route: DOSED FEED                                                                                                 Time: 22:48:35  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE FEMALE                        CONTROL      6250PPM      12500PPM                                               
                                                   FEMALE       FEMALE       FEMALE                                                 
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                             (1)          (2)                                                 
      Cornea, Inflammation, Chronic                                 1 (100%)                                                        
      Cornea, Inflammation, Chronic Active                                       1 (50%)                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (50)         (14)         (49)                                                
      Cyst                                             1 (2%)                                                                       
      Embolus Bacterial                                                          1 (2%)                                             
      Hydronephrosis                                   1 (2%)                    1 (2%)                                             
      Infiltration Cellular, Lymphocyte               14 (28%)      5 (36%)      9 (18%)                                            
      Inflammation, Acute, Multifocal                                            1 (2%)                                             
      Inflammation, Chronic, Multifocal                1 (2%)                                                                       
      Metaplasia, Osseous                                           1 (7%)                                                          
      Glomerulus, Amyloid Deposition                   3 (6%)                                                                       
      Glomerulus, Inflammation, Chronic                                          1 (2%)                                             
      Renal Tubule, Degeneration, Focal                4 (8%)       1 (7%)                                                          
   Urinary Bladder                                    (49)         (6)          (47)                                                
      Hemorrhage                                                                 1 (2%)                                             
      Infiltration Cellular, Lymphocyte               20 (41%)      2 (33%)     21 (45%)                                            
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    7                                                              
NTP Experiment-Test: 05706-01         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                              4,4-DIAMINO-2,2-STILBENEDISULFONIC ACID                          Date: 09/18/94  
Route: DOSED FEED                                                                                                 Time: 22:48:35  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                          CONTROL      6250PPM      12500PPM                                               
                                                   MALE         MALE         MALE                                                   
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY                                                                                                                 
  Animals Initially In Study                          60           60           60                                                  
  Scheduled Sacrifice                                 10           10           10                                                  
  Early Deaths                                                                                                                      
    Moribund                                           3            3            6                                                  
    Dead                                               4            6            2                                                  
  Survivors                                                                                                                         
    Terminal Sacrifice                                43           40           42                                                  
  Missing                                                           1                                                               
  Animals Examined Microscopically                    50           50           50                                                  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Intestine Large, Cecum                             (48)         (4)          (48)                                                
      Peyer's Patch, Hyperplasia, Lymphoid             7 (15%)                  11 (23%)                                            
   Intestine Large, Rectum                            (48)         (7)          (47)                                                
      Serosa, Inflammation, Suppurative                1 (2%)                                                                       
   Intestine Small, Ileum                             (48)         (5)          (48)                                                
      Peyer's Patch, Hyperplasia, Lymphoid                          1 (20%)      1 (2%)                                             
   Intestine Small, Jejunum                           (47)         (14)         (48)                                                
      Hyperplasia, Neutrophil                                       1 (7%)                                                          
      Ulcer                                                                      1 (2%)                                             
      Peyer's Patch, Hyperplasia, Lymphoid             6 (13%)      3 (21%)      8 (17%)                                            
      Serosa, Inflammation, Chronic Active                                       1 (2%)                                             
   Liver                                              (50)         (49)         (50)                                                
      Amyloid Deposition                                            1 (2%)                                                          
      Cyst                                             1 (2%)                    1 (2%)                                             
      Cytomegaly, Focal                                4 (8%)                    2 (4%)                                             
      Fatty Change, Diffuse                            1 (2%)                                                                       
      Fatty Change, Focal                              2 (4%)                    2 (4%)                                             
      Granuloma, Multifocal                            1 (2%)                                                                       
      Hematopoietic Cell Proliferation                              1 (2%)       2 (4%)                                             
      Infarct                                          1 (2%)       1 (2%)       1 (2%)                                             
      Infiltration Cellular, Lymphocyte                3 (6%)       1 (2%)                                                          
      Infiltration Cellular, Mixed Cell                             1 (2%)       1 (2%)                                             
      Mitotic Alteration                               1 (2%)                    1 (2%)                                             
      Mixed Cell Focus                                 1 (2%)                                                                       
      Necrosis, Focal                                  1 (2%)       3 (6%)       1 (2%)                                             
      Syncytial Alteration                             1 (2%)                                                                       
      Bile Duct, Hyperplasia                                                     1 (2%)                                             
      Centrilobular, Cytomegaly, Diffuse               1 (2%)                                                                       
      Centrilobular, Necrosis, Diffuse                 1 (2%)                                                                       
      Hepatocyte, Hyperplasia, Focal                                1 (2%)                                                          
      Portal, Fibrosis                                                           1 (2%)                                             
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    8                                                              
NTP Experiment-Test: 05706-01         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                              4,4-DIAMINO-2,2-STILBENEDISULFONIC ACID                          Date: 09/18/94  
Route: DOSED FEED                                                                                                 Time: 22:48:35  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                          CONTROL      6250PPM      12500PPM                                               
                                                   MALE         MALE         MALE                                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
      Portal, Inflammation, Chronic                    1 (2%)                                                                       
      Serosa, Infiltration Cellular, Histiocyte                     1 (2%)                                                          
   Mesentery                                          (1)                       (1)                                                 
      Infiltration Cellular, Lymphocyte                1 (100%)                                                                     
   Pancreas                                           (49)         (7)          (50)                                                
      Infiltration Cellular, Multifocal, Lymphocyte    4 (8%)                    1 (2%)                                             
      Inflammation, Chronic Active                                               1 (2%)                                             
      Acinus, Atrophy                                                            1 (2%)                                             
   Salivary Glands                                    (50)         (7)          (49)                                                
      Infiltration Cellular, Multifocal, Lymphocyte   13 (26%)                  10 (20%)                                            
   Stomach, Forestomach                               (49)         (6)          (50)                                                
      Abscess                                          1 (2%)                                                                       
      Serosa, Inflammation, Chronic Active                                       1 (2%)                                             
   Stomach, Glandular                                 (49)         (6)          (50)                                                
      Inflammation, Chronic Active                                  1 (17%)                                                         
      Epithelium, Hyperplasia, Focal                                1 (17%)                                                         
      Mucosa, Vacuolization Cytoplasmic                1 (2%)                                                                       
   Tooth                                                                        (3)                                                 
      Abscess                                                                    1 (33%)                                            
      Dysplasia                                                                  2 (67%)                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Blood Vessel                                                                 (1)                                                 
      Aorta, Inflammation, Chronic Active                                        1 (100%)                                           
   Heart                                              (50)         (8)          (50)                                                
      Atrium, Thrombosis                               1 (2%)                                                                       
      Myocardium, Fibrosis                                          1 (13%)                                                         
      Myocardium, Mineralization, Multifocal                                     1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Gland                                      (50)         (7)          (50)                                                
      Capsule, Ectopic Tissue                                                    1 (2%)                                             
      Capsule, Hyperplasia                             1 (2%)                                                                       
      Capsule, Hyperplasia, Multifocal                30 (60%)      2 (29%)     38 (76%)                                            
   Adrenal Gland, Cortex                              (50)         (7)          (50)                                                
      Hyperplasia, Focal                               2 (4%)                    3 (6%)                                             
      Hypertrophy, Focal                               6 (12%)                  12 (24%)                                            
   Adrenal Gland, Medulla                             (50)         (6)          (48)                                                
      Hyperplasia, Focal                                                         1 (2%)                                             
   Islets, Pancreatic                                 (49)         (7)          (50)                                                
      Hyperplasia                                      7 (14%)      1 (14%)      2 (4%)                                             
   Pituitary Gland                                    (48)         (7)          (44)                                                
      Pars Distalis, Cyst                                                        1 (2%)                                             
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page    9                                                              
NTP Experiment-Test: 05706-01         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                              4,4-DIAMINO-2,2-STILBENEDISULFONIC ACID                          Date: 09/18/94  
Route: DOSED FEED                                                                                                 Time: 22:48:35  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                          CONTROL      6250PPM      12500PPM                                               
                                                   MALE         MALE         MALE                                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ENDOCRINE SYSTEM - CONT                                                                                                             
      Pars Distalis, Hyperplasia, Focal                             1 (14%)                                                         
   Thyroid Gland                                      (50)         (7)          (49)                                                
      Infiltration Cellular, Lymphocyte                1 (2%)       1 (14%)                                                         
      Follicle, Cyst, Multiple                         1 (2%)                                                                       
      Follicular Cell, Hyperplasia, Focal                                        1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Epididymis                                         (50)         (9)          (50)                                                
      Granuloma Sperm                                  1 (2%)                                                                       
      Serosa, Necrosis, Focal                                                    1 (2%)                                             
   Penis                                              (3)          (1)          (4)                                                 
      Congestion                                                                 1 (25%)                                            
      Inflammation, Acute                                           1 (100%)     1 (25%)                                            
      Necrosis                                         2 (67%)                   1 (25%)                                            
   Preputial Gland                                    (6)          (7)          (11)                                                
      Abscess                                                                    4 (36%)                                            
      Cyst                                                          1 (14%)      4 (36%)                                            
      Dilatation                                       4 (67%)      3 (43%)      2 (18%)                                            
      Hyperplasia                                      1 (17%)                                                                      
      Inflammation, Chronic                            1 (17%)      5 (71%)      3 (27%)                                            
   Prostate                                           (50)         (7)          (48)                                                
      Inflammation, Acute                              3 (6%)                    1 (2%)                                             
      Inflammation, Chronic Active                                               1 (2%)                                             
   Seminal Vesicle                                    (49)         (9)          (50)                                                
      Fibrosis                                         3 (6%)       1 (11%)                                                         
      Inflammation, Acute                              1 (2%)                                                                       
      Inflammation, Chronic                            2 (4%)       1 (11%)      2 (4%)                                             
   Testes                                             (50)         (9)          (50)                                                
      Mineralization, Focal                                                      1 (2%)                                             
      Spermatocele                                     1 (2%)                                                                       
      Seminiferous Tubule, Atrophy                                  1 (11%)                                                         
      Seminiferous Tubule, Dilatation                  1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (50)         (8)          (50)                                                
      Hyperplasia                                                                1 (2%)                                             
      Hyperplasia, Neutrophil                          2 (4%)       2 (25%)      5 (10%)                                            
      Erythroid Cell, Depletion Cellular                                         1 (2%)                                             
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   10                                                              
NTP Experiment-Test: 05706-01         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                              4,4-DIAMINO-2,2-STILBENEDISULFONIC ACID                          Date: 09/18/94  
Route: DOSED FEED                                                                                                 Time: 22:48:35  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                          CONTROL      6250PPM      12500PPM                                               
                                                   MALE         MALE         MALE                                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - CONT                                                                                                         
   Lymph Node                                         (48)         (13)         (47)                                                
      Hyperplasia, Lymphoid                            2 (4%)                                                                       
      Hyperplasia, Plasma Cell                                                   2 (4%)                                             
      Axillary, Hemorrhage                             1 (2%)                                                                       
      Axillary, Hyperplasia, Lymphoid                  1 (2%)                                                                       
      Iliac, Hyperplasia, Lymphoid                     1 (2%)                                                                       
      Inguinal, Hyperplasia, Lymphoid                  1 (2%)                    4 (9%)                                             
      Inguinal, Hyperplasia, Plasma Cell                            1 (8%)       1 (2%)                                             
      Inguinal, Pigmentation, Hemosiderin              2 (4%)                                                                       
      Lumbar, Hyperplasia, Plasma Cell                                           1 (2%)                                             
      Mandibular, Hemorrhage                                                     1 (2%)                                             
      Mandibular, Hyperplasia, Lymphoid                3 (6%)                                                                       
      Mandibular, Pigmentation, Hemosiderin            1 (2%)                                                                       
      Renal, Hyperplasia, Plasma Cell                                            1 (2%)                                             
   Lymph Node, Mesenteric                             (45)         (10)         (45)                                                
      Hematopoietic Cell Proliferation                                           1 (2%)                                             
      Hemorrhage                                      13 (29%)      3 (30%)     10 (22%)                                            
      Hyperplasia, Lymphoid                            8 (18%)      2 (20%)      9 (20%)                                            
   Spleen                                             (50)         (39)         (50)                                                
      Congestion                                       1 (2%)                                                                       
      Depletion Lymphoid                                                         2 (4%)                                             
      Dysplasia                                                                  1 (2%)                                             
      Hematopoietic Cell Proliferation                11 (22%)      4 (10%)     12 (24%)                                            
      Hyperplasia, Lymphoid                            2 (4%)       3 (8%)       1 (2%)                                             
      Pigmentation, Hemosiderin                        1 (2%)                    1 (2%)                                             
   Thymus                                             (44)         (4)          (45)                                                
      Depletion Lymphoid                               3 (7%)       2 (50%)      7 (16%)                                            
      Epithelial Cell, Hyperplasia                                               1 (2%)                                             
      Thymocyte, Necrosis                              4 (9%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Skin                                               (50)         (20)         (50)                                                
      Inflammation, Acute                                                        2 (4%)                                             
      Inflammation, Chronic                            6 (12%)      1 (5%)       3 (6%)                                             
      Inflammation, Chronic Active                     2 (4%)       1 (5%)                                                          
      Ulcer                                            5 (10%)      1 (5%)       2 (4%)                                             
      Epithelium, Hyperplasia                          1 (2%)       1 (5%)                                                          
      Hair Follicle, Atrophy                           1 (2%)                                                                       
      Prepuce, Inflammation, Acute                                               1 (2%)                                             
      Prepuce, Ulcer                                                             1 (2%)                                             
      Subcutaneous Tissue, Abscess                     1 (2%)                                                                       
      Subcutaneous Tissue, Edema                       1 (2%)       1 (5%)                                                          
____________________________________________________________________________________________________________________________________
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   11                                                              
NTP Experiment-Test: 05706-01         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                              4,4-DIAMINO-2,2-STILBENEDISULFONIC ACID                          Date: 09/18/94  
Route: DOSED FEED                                                                                                 Time: 22:48:35  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                          CONTROL      6250PPM      12500PPM                                               
                                                   MALE         MALE         MALE                                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (50)         (41)         (49)                                                
      Hemorrhage                                                    1 (2%)                                                          
      Mineralization, Multifocal                      38 (76%)     37 (90%)     18 (37%)                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (48)         (50)                                                
      Giant Cell                                       2 (4%)                                                                       
      Leukocytosis                                                  2 (4%)                                                          
      Alveolar Epithelium, Hyperplasia, Focal          3 (6%)       2 (4%)       2 (4%)                                             
      Alveolus, Infiltration Cellular, Histiocyte      2 (4%)       1 (2%)                                                          
      Artery, Hypertrophy, Multifocal                               1 (2%)                                                          
      Bronchiole, Inflammation, Chronic                2 (4%)                                                                       
      Interstitium, Fibrosis                                                     1 (2%)                                             
      Peribronchial, Infiltration Cellular,                                                                                         
          Lymphocyte                                   2 (4%)                                                                       
      Peribronchiolar, Infiltration Cellular,                                                                                       
          Lymphocyte                                   1 (2%)       1 (2%)                                                          
      Perivascular, Infiltration Cellular,                                                                                          
          Lymphocyte                                                1 (2%)       1 (2%)                                             
   Nose                                               (50)         (8)          (49)                                                
      Mucosa, Degeneration, Hyaline                    3 (6%)                    4 (8%)                                             
      Mucosa, Inflammation, Acute                                                1 (2%)                                             
      Mucosa, Inflammation, Chronic Active             3 (6%)                                                                       
      Respiratory Epithelium, Hyperplasia              6 (12%)      1 (13%)      5 (10%)                                            
   Trachea                                            (50)         (7)          (50)                                                
      Infiltration Cellular, Lymphocyte                             1 (14%)                                                         
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                (1)          (1)                                                              
      Conjunctiva, Inflammation, Chronic               1 (100%)                                                                     
      Lens, Cataract                                                1 (100%)                                                        
____________________________________________________________________________________________________________________________________
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   12                                                              
NTP Experiment-Test: 05706-01         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: CHRONIC                              4,4-DIAMINO-2,2-STILBENEDISULFONIC ACID                          Date: 09/18/94  
Route: DOSED FEED                                                                                                 Time: 22:48:35  
____________________________________________________________________________________________________________________________________
         B6C3F1 MICE MALE                          CONTROL      6250PPM      12500PPM                                               
                                                   MALE         MALE         MALE                                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (50)         (14)         (50)                                                
      Hydronephrosis                                                             1 (2%)                                             
      Hyperplasia, Atypical, Focal                                               1 (2%)                                             
      Infarct                                                       1 (7%)                                                          
      Infiltration Cellular, Lymphocyte               18 (36%)      1 (7%)      13 (26%)                                            
      Infiltration Cellular, Mixed Cell                1 (2%)                    1 (2%)                                             
      Nephropathy, Chronic                             8 (16%)                   7 (14%)                                            
      Cortex, Cyst                                     1 (2%)       2 (14%)      2 (4%)                                             
      Cortex, Mineralization, Multifocal               5 (10%)      2 (14%)      1 (2%)                                             
      Glomerulus, Amyloid Deposition                   1 (2%)                                                                       
      Pelvis, Fibrosis                                                           1 (2%)                                             
      Pelvis, Inflammation, Acute                      1 (2%)                    3 (6%)                                             
      Pelvis, Inflammation, Chronic Active             1 (2%)                                                                       
      Renal Tubule, Degeneration, Focal                2 (4%)                    3 (6%)                                             
      Renal Tubule, Dilatation                                                   2 (4%)                                             
   Ureter                                                                       (1)                                                 
      Hyperplasia                                                                1 (100%)                                           
   Urethra                                            (2)                       (2)                                                 
      Inflammation, Acute                              1 (50%)                   1 (50%)                                            
      Bulbourethral Gland, Ectasia                     1 (50%)                                                                      
      Bulbourethral Gland, Inflammation, Acute                                   1 (50%)                                            
   Urinary Bladder                                    (48)         (7)          (49)                                                
      Dilatation                                                                 2 (4%)                                             
      Infiltration Cellular, Lymphocyte                5 (10%)      1 (14%)      6 (12%)                                            
      Inflammation, Acute                              1 (2%)                    1 (2%)                                             
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                             Page   13                                                              
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                             ----------              END OF REPORT             ----------                                           
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