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TDMS Study 50215-03 Pathology Tables

NTP Experiment-Test: 50215-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                           ACRYLONITRILE                                       Date: 10/31/00
Route: GAVAGE                                                                                                     Time: 12:42:06

                                                          MICE FINAL#1




       Facility:  Battelle Columbus Laboratory

       Chemical CAS #:  107-13-1

       Lock Date:  11/03/99

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All


































a  Number of animals examined microscopically at site and number of animals with lesion

                                                              Page   1


NTP Experiment-Test: 50215-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                           ACRYLONITRILE                                       Date: 10/31/00  
Route: GAVAGE                                                                                                     Time: 12:42:06  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/KG       2.5         10 MG/KG     20 MG/KG                                  
                                                                MG/KG                                                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially in Study                          50           50           50           50                                     
  Early Deaths                                                                                                                      
    Natural Death                                      6           15           10           25                                     
    Moribund Sacrifice                                 5            3            1            1                                     
    Dosing Accident                                                                           1                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                39           32           39           22                                     
    Moribund Sacrifice                                                                        1                                     
                                                                                                                                    
  Animals Examined Microscopically                    50           50           50           50                                     
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Intestine Large, Colon                             (50)         (50)         (50)         (50)                                   
      Histiocytic Sarcoma                                                        1 (2%)                                             
      Leiomyosarcoma                                                             1 (2%)                                             
   Intestine Large, Rectum                            (50)         (50)         (49)         (50)                                   
   Intestine Large, Cecum                             (50)         (50)         (50)         (50)                                   
      Histiocytic Sarcoma                                                        1 (2%)                                             
   Intestine Small, Duodenum                          (50)         (50)         (49)         (50)                                   
      Histiocytic Sarcoma                                                        1 (2%)                                             
   Intestine Small, Jejunum                           (50)         (50)         (50)         (50)                                   
      Histiocytic Sarcoma                                                        1 (2%)                                             
   Intestine Small, Ileum                             (50)         (50)         (50)         (50)                                   
   Liver                                              (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                  1 (2%)                                             
      Hemangiosarcoma                                  2 (4%)       1 (2%)       1 (2%)                                             
      Hepatocellular Carcinoma                         7 (14%)      3 (6%)       6 (12%)      2 (4%)                                
      Hepatocellular Adenoma                           8 (16%)     10 (20%)     10 (20%)      9 (18%)                               
      Hepatocellular Adenoma, Multiple                 6 (12%)      4 (8%)       5 (10%)      1 (2%)                                
      Hepatocholangiocarcinoma                                                                1 (2%)                                
      Histiocytic Sarcoma                              1 (2%)                    1 (2%)                                             
      Lipoma                                                                     1 (2%)                                             
      Sarcoma, Metastatic, Mesentery                                             1 (2%)                                             
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                              1 (2%)       1 (2%)       7 (14%)                               
   Mesentery                                          (9)          (10)         (10)         (10)                                   
      Fibrosarcoma, Metastatic, Skin                                1 (10%)                                                         
      Hemangiosarcoma                                  1 (11%)                   1 (10%)                                            
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   2                                                               
                                                                                                                                   
NTP Experiment-Test: 50215-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                           ACRYLONITRILE                                       Date: 10/31/00  
Route: GAVAGE                                                                                                     Time: 12:42:06  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/KG       2.5         10 MG/KG     20 MG/KG                                  
                                                                MG/KG                                                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - cont                                                                                                            
      Sarcoma                                                                    1 (10%)                                            
      Sarcoma, Metastatic, Skin                        1 (11%)                                1 (10%)                               
      Fat, Squamous Cell Carcinoma, Metastatic,                                                                                     
           Stomach, Forestomach                                                               2 (20%)                               
   Pancreas                                           (50)         (50)         (50)         (50)                                   
      Sarcoma, Metastatic, Skin                                                               1 (2%)                                
   Salivary Glands                                    (50)         (50)         (49)         (50)                                   
      Sarcoma, Metastatic, Skin                        1 (2%)                                                                       
   Stomach, Forestomach                               (50)         (50)         (50)         (50)                                   
      Fibrosarcoma, Metastatic, Skin                                1 (2%)                                                          
      Squamous Cell Carcinoma                                       1 (2%)       1 (2%)      11 (22%)                               
      Squamous Cell Papilloma                          2 (4%)       6 (12%)     20 (40%)     10 (20%)                               
      Squamous Cell Papilloma, Multiple                1 (2%)                    4 (8%)       9 (18%)                               
   Stomach, Glandular                                 (50)         (50)         (50)         (50)                                   
      Fibrosarcoma, Metastatic, Skin                                1 (2%)                                                          
      Histiocytic Sarcoma                              1 (2%)                    1 (2%)                                             
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                                                        3 (6%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                  1 (2%)                                             
      Fibrosarcoma, Metastatic, Skin                                                          1 (2%)                                
      Sarcoma, Metastatic, Skin                        1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (50)         (50)         (50)         (50)                                   
      Histiocytic Sarcoma                              1 (2%)                                                                       
      Bilateral, Histiocytic Sarcoma                                             1 (2%)                                             
      Subcapsular, Adenoma, Multiple                                                          1 (2%)                                
   Adrenal Medulla                                    (50)         (50)         (50)         (50)                                   
      Pheochromocytoma Benign                                       1 (2%)                                                          
      Bilateral, Pheochromocytoma Benign                                         1 (2%)                                             
   Islets, Pancreatic                                 (50)         (50)         (50)         (50)                                   
      Adenoma                                                       1 (2%)       1 (2%)                                             
   Pituitary Gland                                    (48)         (49)         (48)         (49)                                   
      Meningioma Malignant, Metastatic, Brain          1 (2%)                                                                       
      Pars Distalis, Adenoma                           2 (4%)       4 (8%)       4 (8%)       2 (4%)                                
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   3                                                               
                                                                                                                                   
NTP Experiment-Test: 50215-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                           ACRYLONITRILE                                       Date: 10/31/00  
Route: GAVAGE                                                                                                     Time: 12:42:06  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/KG       2.5         10 MG/KG     20 MG/KG                                  
                                                                MG/KG                                                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ENDOCRINE SYSTEM - cont                                                                                                             
   Thyroid Gland                                      (50)         (50)         (49)         (50)                                   
      Follicular Cell, Adenoma                         2 (4%)       1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Clitoral Gland                                     (50)         (48)         (49)         (48)                                   
   Ovary                                              (50)         (50)         (50)         (50)                                   
      Cystadenoma                                      8 (16%)      4 (8%)       2 (4%)       3 (6%)                                
      Granulosa Cell Tumor Malignant                                             1 (2%)                                             
      Granulosa Cell Tumor Benign                                                3 (6%)       1 (2%)                                
      Hemangioma                                                                 1 (2%)       2 (4%)                                
      Histiocytic Sarcoma                              1 (2%)                                                                       
      Luteoma                                                       1 (2%)                                                          
   Oviduct                                                         (1)                       (1)                                    
   Uterus                                             (50)         (50)         (50)         (50)                                   
      Hemangioma                                                    1 (2%)                    2 (4%)                                
      Histiocytic Sarcoma                              1 (2%)                                                                       
      Leiomyoma                                                     1 (2%)                                                          
      Polyp Stromal                                                 1 (2%)       3 (6%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (50)         (50)         (50)         (50)                                   
      Histiocytic Sarcoma                                                        1 (2%)                                             
   Lymph Node                                         (37)         (32)         (30)         (25)                                   
      Mediastinal, Alveolar/Bronchiolar Carcinoma,                                                                                  
           Metastatic, Lung                                                      1 (3%)                                             
      Mediastinal, Histiocytic Sarcoma                 1 (3%)                                                                       
      Mediastinal, Liposarcoma, Metastatic, Skin                                 1 (3%)                                             
      Mediastinal, Sarcoma, Metastatic, Mesentery                                1 (3%)                                             
      Pancreatic, Histiocytic Sarcoma                  1 (3%)                                                                       
   Lymph Node, Mandibular                             (49)         (49)         (47)         (50)                                   
      Carcinoma, Metastatic, Harderian Gland                                     1 (2%)       1 (2%)                                
      Histiocytic Sarcoma                              1 (2%)                                                                       
   Lymph Node, Mesenteric                             (50)         (49)         (50)         (47)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   4                                                               
                                                                                                                                   
NTP Experiment-Test: 50215-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                           ACRYLONITRILE                                       Date: 10/31/00  
Route: GAVAGE                                                                                                     Time: 12:42:06  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/KG       2.5         10 MG/KG     20 MG/KG                                  
                                                                MG/KG                                                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - cont                                                                                                         
           Lung                                                                  1 (2%)                                             
      Fibrosarcoma, Metastatic, Skin                                                          1 (2%)                                
      Hemangiosarcoma                                               1 (2%)                                                          
      Histiocytic Sarcoma                              1 (2%)                    1 (2%)                                             
      Sarcoma, Metastatic, Skin                                                               1 (2%)                                
   Spleen                                             (50)         (50)         (50)         (50)                                   
      Hemangiosarcoma                                               1 (2%)                                                          
      Histiocytic Sarcoma                              1 (2%)                    1 (2%)                                             
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                                                        1 (2%)                                
   Thymus                                             (50)         (50)         (47)         (50)                                   
      Fibrosarcoma, Metastatic, Skin                                                          1 (2%)                                
      Histiocytic Sarcoma                                                        1 (2%)                                             
      Thymoma Benign                                                1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (50)         (50)         (50)         (50)                                   
      Carcinoma                                                                  1 (2%)                                             
      Fibroadenoma                                                                            1 (2%)                                
   Skin                                               (50)         (50)         (50)         (50)                                   
      Basal Cell Carcinoma                                          1 (2%)                                                          
      Subcutaneous Tissue, Alveolar/Bronchiolar                                                                                     
          Carcinoma, Metastatic, Lung                  1 (2%)                                                                       
      Subcutaneous Tissue, Fibrosarcoma                2 (4%)       2 (4%)       3 (6%)       2 (4%)                                
      Subcutaneous Tissue, Fibrosarcoma, Multiple                   1 (2%)                                                          
      Subcutaneous Tissue, Fibrous Histiocytoma                     1 (2%)                                                          
      Subcutaneous Tissue, Hemangiosarcoma             1 (2%)                                                                       
      Subcutaneous Tissue, Histiocytic Sarcoma         1 (2%)                                                                       
      Subcutaneous Tissue, Sarcoma                     1 (2%)       1 (2%)       1 (2%)       1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Skeletal Muscle                                    (3)          (3)          (2)          (1)                                    
      Histiocytic Sarcoma                              1 (33%)                                                                      
      Sarcoma, Metastatic, Mesentery                                             1 (50%)                                            
      Sarcoma, Metastatic, Skin                        1 (33%)                                                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   5                                                               
                                                                                                                                   
NTP Experiment-Test: 50215-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                           ACRYLONITRILE                                       Date: 10/31/00  
Route: GAVAGE                                                                                                     Time: 12:42:06  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/KG       2.5         10 MG/KG     20 MG/KG                                  
                                                                MG/KG                                                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (50)         (50)         (50)         (50)                                   
      Meningioma Malignant                             1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Adenoma                     4 (8%)       1 (2%)       8 (16%)      5 (10%)                               
      Alveolar/Bronchiolar Carcinoma                   2 (4%)       5 (10%)      6 (12%)      4 (8%)                                
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                        1 (2%)       1 (2%)       1 (2%)       1 (2%)                                
      Basal Cell Carcinoma, Metastatic, Skin                        1 (2%)                                                          
      Carcinoma, Metastatic, Harderian Gland           1 (2%)                    3 (6%)       1 (2%)                                
      Fibrosarcoma, Metastatic, Skin                                             1 (2%)       1 (2%)                                
      Hepatocellular Carcinoma, Metastatic, Liver                   1 (2%)       2 (4%)                                             
      Hepatocholangiocarcinoma, Metastatic, Liver                                             1 (2%)                                
      Histiocytic Sarcoma                              1 (2%)                    1 (2%)                                             
      Sarcoma, Metastatic, Skin                        1 (2%)                    1 (2%)                                             
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                              1 (2%)                    1 (2%)                                
      Bronchus, Adenoma                                1 (2%)                                                                       
      Mediastinum, Alveolar/Bronchiolar Carcinoma,                                                                                  
           Metastatic, Lung                                         1 (2%)       1 (2%)                                             
      Mediastinum, Fibrosarcoma, Metastatic, Skin                   1 (2%)                                                          
      Mediastinum, Sarcoma, Metastatic, Skin           1 (2%)                    1 (2%)                                             
   Nose                                               (50)         (50)         (50)         (50)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Harderian Gland                                    (49)         (50)         (49)         (49)                                   
      Adenoma                                          8 (16%)     10 (20%)     21 (43%)     19 (39%)                               
      Carcinoma                                        1 (2%)                    3 (6%)       2 (4%)                                
      Bilateral, Adenoma                               2 (4%)                    4 (8%)       4 (8%)                                
   Zymbal's Gland                                     (1)                                                                           
      Carcinoma                                        1 (100%)                                                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   6                                                               
                                                                                                                                   
NTP Experiment-Test: 50215-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                           ACRYLONITRILE                                       Date: 10/31/00  
Route: GAVAGE                                                                                                     Time: 12:42:06  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/KG       2.5         10 MG/KG     20 MG/KG                                  
                                                                MG/KG                                                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                        1 (2%)                    1 (2%)                                             
      Histiocytic Sarcoma                              1 (2%)                    1 (2%)                                             
      Capsule, Squamous Cell Carcinoma, Metastatic,                                                                                 
           Stomach, Forestomach                                                               1 (2%)                                
      Renal Tubule, Adenoma                                         1 (2%)       1 (2%)       1 (2%)                                
   Urinary Bladder                                    (50)         (49)         (50)         (50)                                   
      Histiocytic Sarcoma                              1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
                                                                                                                                    
   Multiple Organs                                   *(50)        *(50)        *(50)        *(50)                                   
      Histiocytic Sarcoma                              1 (2%)                    1 (2%)                                             
      Lymphoma Malignant                               4 (8%)       5 (10%)      7 (14%)      6 (12%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   7                                                               
                                                                                                                                   
NTP Experiment-Test: 50215-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                           ACRYLONITRILE                                       Date: 10/31/00  
Route: GAVAGE                                                                                                     Time: 12:42:06  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE FEMALE                        0 MG/KG       2.5         10 MG/KG     20 MG/KG                                  
                                                                MG/KG                                                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)            39          41          50          40                                       
     Total Primary Neoplasms                           68          71         123          99                                       
                                                                                                                                    
   Total Animals with Benign Neoplasms                 29          33          45          33                                       
     Total Benign Neoplasms                            44          48          89          70                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms              24          22          30          24                                       
     Total Malignant Neoplasms                         24          23          34          29                                       
                                                                                                                                    
   Total Animals with Metastatic Neoplasms              4           5          10          12                                       
     Total Metastatic Neoplasm                         11          10          21          26                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms                                                                                           
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                                                                                              
     Total Uncertain Neoplasms                                                                                                      
                                                                                                                                    
                                                                                                                                    
____________________________________________________________________________________________________________________________________
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   8                                                               
                                                                                                                                   
NTP Experiment-Test: 50215-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                           ACRYLONITRILE                                       Date: 10/31/00  
Route: GAVAGE                                                                                                     Time: 12:42:06  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/KG       2.5         10 MG/KG     20 MG/KG                                  
                                                                MG/KG                                                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially in Study                          50           50           50           50                                     
  Early Deaths                                                                                                                      
    Natural Death                                     11            3            8           32                                     
    Moribund Sacrifice                                 1            3            3            4                                     
    Dosing Accident                                                 2                                                               
  Survivors                                                                                                                         
    Terminal Sacrifice                                38           42           39           14                                     
                                                                                                                                    
  Animals Examined Microscopically                    50           50           50           50                                     
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Gallbladder                                        (50)         (49)         (50)         (48)                                   
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                                                        1 (2%)                                
   Intestine Large, Colon                             (50)         (50)         (50)         (50)                                   
   Intestine Small, Duodenum                          (50)         (50)         (50)         (50)                                   
      Polyp Adenomatous                                1 (2%)       3 (6%)                                                          
   Intestine Small, Jejunum                           (50)         (50)         (50)         (50)                                   
      Carcinoma                                        2 (4%)       1 (2%)                                                          
   Intestine Small, Ileum                             (50)         (50)         (50)         (50)                                   
      Hemangioma                                                                              1 (2%)                                
      Hemangiosarcoma                                                                         1 (2%)                                
   Liver                                              (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                     1 (2%)       1 (2%)       1 (2%)                                
      Hemangiosarcoma                                  1 (2%)       1 (2%)       2 (4%)       1 (2%)                                
      Hemangiosarcoma, Multiple                        1 (2%)                                 1 (2%)                                
      Hepatoblastoma                                                1 (2%)                                                          
      Hepatocellular Carcinoma                        11 (22%)      9 (18%)     13 (26%)      6 (12%)                               
      Hepatocellular Carcinoma, Multiple               3 (6%)       1 (2%)       4 (8%)                                             
      Hepatocellular Adenoma                          13 (26%)     21 (42%)     11 (22%)     10 (20%)                               
      Hepatocellular Adenoma, Multiple                10 (20%)     11 (22%)     18 (36%)      4 (8%)                                
      Histiocytic Sarcoma                                                                     1 (2%)                                
      Osteosarcoma, Metastatic, Bone                                1 (2%)                                                          
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                                           4 (8%)       4 (8%)                                
   Mesentery                                          (8)          (6)          (8)          (3)                                    
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                     1 (17%)                                                         
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   9                                                               
                                                                                                                                   
NTP Experiment-Test: 50215-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                           ACRYLONITRILE                                       Date: 10/31/00  
Route: GAVAGE                                                                                                     Time: 12:42:06  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/KG       2.5         10 MG/KG     20 MG/KG                                  
                                                                MG/KG                                                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - cont                                                                                                            
      Hemangioma                                                    1 (17%)                                                         
      Sarcoma                                          1 (13%)                                                                      
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                                           1 (13%)                                            
      Fat, Carcinoma, Metastatic, Harderian Gland                                1 (13%)                                            
      Fat, Squamous Cell Carcinoma, Metastatic,                                                                                     
           Stomach, Forestomach                                                  2 (25%)      2 (67%)                               
   Pancreas                                           (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                               1 (2%)                                
      Sarcoma, Metastatic, Mesentery                   1 (2%)                                                                       
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                                           3 (6%)       2 (4%)                                
   Salivary Glands                                    (50)         (50)         (50)         (50)                                   
   Stomach, Forestomach                               (50)         (50)         (50)         (50)                                   
      Squamous Cell Carcinoma                                                    8 (16%)      9 (18%)                               
      Squamous Cell Papilloma                          3 (6%)       4 (8%)      17 (34%)     16 (32%)                               
      Squamous Cell Papilloma, Multiple                                          2 (4%)       9 (18%)                               
   Stomach, Glandular                                 (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                  1 (2%)                                             
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                                           3 (6%)       2 (4%)                                
   Tongue                                                                                    (2)                                    
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                               1 (50%)                               
      Squamous Cell Carcinoma                                                                 1 (50%)                               
   Tooth                                              (9)          (5)          (4)          (3)                                    
      Peridontal Tissue, Sarcoma                                                              1 (33%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                     1 (2%)       1 (2%)                                             
      Hemangioma                                                                              1 (2%)                                
      Hemangiosarcoma                                                            1 (2%)                                             
      Schwannoma Malignant                             1 (2%)                                                                       
      Pericardium, Hepatocellular Carcinoma,                                                                                        
          Metastatic, Liver                            1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  10                                                               
                                                                                                                                   
NTP Experiment-Test: 50215-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                           ACRYLONITRILE                                       Date: 10/31/00  
Route: GAVAGE                                                                                                     Time: 12:42:06  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/KG       2.5         10 MG/KG     20 MG/KG                                  
                                                                MG/KG                                                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (50)         (50)         (50)         (50)                                   
      Adenoma                                                                    1 (2%)                                             
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                  1 (2%)       1 (2%)                                
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                                           1 (2%)                                             
      Subcapsular, Adenoma                             3 (6%)                    3 (6%)                                             
   Adrenal Medulla                                    (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                  1 (2%)       1 (2%)                                
   Islets, Pancreatic                                 (50)         (50)         (50)         (50)                                   
      Adenoma                                          2 (4%)                    2 (4%)                                             
   Pituitary Gland                                    (49)         (46)         (47)         (47)                                   
      Pars Intermedia, Adenoma                         1 (2%)                                                                       
   Thyroid Gland                                      (50)         (50)         (50)         (50)                                   
      Follicular Cell, Adenoma                                      1 (2%)       1 (2%)                                             
      Follicular Cell, Carcinoma                                    1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   Peritoneum                                                                                (1)                                    
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                                                        1 (100%)                              
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Coagulating Gland                                  (1)                       (2)                                                 
   Epididymis                                         (50)         (50)         (50)         (50)                                   
      Sarcoma, Metastatic, Mesentery                   1 (2%)                                                                       
   Preputial Gland                                    (49)         (50)         (50)         (50)                                   
      Hemangioma                                                                 1 (2%)                                             
   Prostate                                           (50)         (50)         (50)         (50)                                   
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                                           1 (2%)                                             
   Seminal Vesicle                                    (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                     1 (2%)                                                          
      Carcinoma                                        1 (2%)                                                                       
      Sarcoma, Metastatic, Mesentery                   1 (2%)                                                                       
   Testes                                             (50)         (50)         (50)         (50)                                   
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  11                                                               
                                                                                                                                   
NTP Experiment-Test: 50215-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                           ACRYLONITRILE                                       Date: 10/31/00  
Route: GAVAGE                                                                                                     Time: 12:42:06  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/KG       2.5         10 MG/KG     20 MG/KG                                  
                                                                MG/KG                                                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
GENITAL SYSTEM - cont                                                                                                               
      Interstitial Cell, Adenoma                                    1 (2%)       1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (50)         (50)         (50)         (50)                                   
      Hemangiosarcoma                                  2 (4%)                                                                       
   Lymph Node                                         (6)          (4)          (6)          (5)                                    
      Bronchial, Squamous Cell Carcinoma,                                                                                           
          Metastatic, Stomach, Forestomach                                                    1 (20%)                               
      Mediastinal, Alveolar/Bronchiolar Carcinoma,                                                                                  
           Metastatic, Lung                            1 (17%)      1 (25%)      1 (17%)      1 (20%)                               
      Mediastinal, Carcinoma, Metastatic,                                                                                           
          Harderian Gland                                                        1 (17%)                                            
      Mediastinal, Hepatocellular Carcinoma,                                                                                        
          Metastatic, Liver                            1 (17%)                                                                      
      Mediastinal, Sarcoma, Metastatic, Mesentery      1 (17%)                                                                      
      Mediastinal, Squamous Cell Carcinoma,                                                                                         
          Metastatic, Stomach, Forestomach                                       1 (17%)                                            
      Pancreatic, Squamous Cell Carcinoma,                                                                                          
          Metastatic, Stomach, Forestomach                                       1 (17%)      1 (20%)                               
      Renal, Alveolar/Bronchiolar Carcinoma,                                                                                        
          Metastatic, Lung                                          1 (25%)                                                         
   Lymph Node, Mandibular                             (49)         (47)         (47)         (47)                                   
   Lymph Node, Mesenteric                             (48)         (48)         (49)         (48)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                  1 (2%)                                             
      Hemangioma                                       2 (4%)       1 (2%)                                                          
      Histiocytic Sarcoma                                                                     1 (2%)                                
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                                                        2 (4%)                                
   Spleen                                             (50)         (50)         (50)         (50)                                   
      Hemangiosarcoma                                  1 (2%)                    1 (2%)                                             
      Histiocytic Sarcoma                                                                     1 (2%)                                
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                                           1 (2%)                                             
   Thymus                                             (47)         (47)         (44)         (46)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                        1 (2%)       1 (2%)       1 (2%)                                             
      Hepatocellular Carcinoma, Metastatic, Liver      1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  12                                                               
                                                                                                                                   
NTP Experiment-Test: 50215-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                           ACRYLONITRILE                                       Date: 10/31/00  
Route: GAVAGE                                                                                                     Time: 12:42:06  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/KG       2.5         10 MG/KG     20 MG/KG                                  
                                                                MG/KG                                                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Skin                                               (50)         (50)         (50)         (50)                                   
      Subcutaneous Tissue, Fibrosarcoma                             1 (2%)                                                          
      Subcutaneous Tissue, Hemangiosarcoma             1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (50)         (50)         (50)         (50)                                   
      Osteosarcoma                                                  1 (2%)                    1 (2%)                                
      Osteosarcoma, Multiple                                        1 (2%)                                                          
      Femur, Fibrosarcoma                              1 (2%)                                                                       
   Skeletal Muscle                                    (1)                       (2)                                                 
      Carcinoma, Metastatic, Stomach, Forestomach                                1 (50%)                                            
      Sarcoma, Metastatic, Mesentery                   1 (100%)                                                                     
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                                           1 (50%)                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Adenoma                    10 (20%)      8 (16%)      4 (8%)       6 (12%)                               
      Alveolar/Bronchiolar Adenoma, Multiple                        1 (2%)                    2 (4%)                                
      Alveolar/Bronchiolar Carcinoma                   4 (8%)       2 (4%)       4 (8%)       2 (4%)                                
      Alveolar/Bronchiolar Carcinoma, Multiple                                   1 (2%)                                             
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                        2 (4%)       2 (4%)       2 (4%)                                             
      Carcinoma, Metastatic, Harderian Gland                                     3 (6%)       1 (2%)                                
      Carcinoma, Metastatic, Thyroid Gland                          1 (2%)                                                          
      Hepatocellular Carcinoma, Metastatic, Liver      5 (10%)      3 (6%)       4 (8%)       2 (4%)                                
      Histiocytic Sarcoma                                                                     1 (2%)                                
      Osteosarcoma, Metastatic, Bone                                1 (2%)                                                          
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                                           4 (8%)       1 (2%)                                
      Mediastinum, Alveolar/Bronchiolar Carcinoma,                                                                                  
           Metastatic, Lung                                         1 (2%)       1 (2%)       1 (2%)                                
      Mediastinum, Hepatocellular Carcinoma,                                                                                        
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  13                                                               
                                                                                                                                   
NTP Experiment-Test: 50215-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                           ACRYLONITRILE                                       Date: 10/31/00  
Route: GAVAGE                                                                                                     Time: 12:42:06  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/KG       2.5         10 MG/KG     20 MG/KG                                  
                                                                MG/KG                                                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
RESPIRATORY SYSTEM - cont                                                                                                           
          Metastatic, Liver                            1 (2%)                                                                       
      Mediastinum, Osteosarcoma, Metastatic, Bone                   1 (2%)                                                          
   Nose                                               (50)         (50)         (50)         (50)                                   
      Carcinoma, Metastatic, Harderian Gland                                     1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Harderian Gland                                    (50)         (50)         (50)         (50)                                   
      Adenoma                                          5 (10%)     16 (32%)     15 (30%)     21 (42%)                               
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                                               1 (2%)                                
      Carcinoma                                        1 (2%)       1 (2%)       4 (8%)       3 (6%)                                
      Bilateral, Adenoma                                                         9 (18%)      6 (12%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (50)         (50)         (50)         (50)                                   
      Alveolar/Bronchiolar Carcinoma, Metastatic,                                                                                   
           Lung                                                     2 (4%)                    1 (2%)                                
      Squamous Cell Carcinoma, Metastatic, Stomach,                                                                                 
           Forestomach                                                           1 (2%)       1 (2%)                                
      Capsule, Carcinoma, Metastatic, Harderian                                                                                     
          Gland                                                                  1 (2%)                                             
      Capsule, Hepatocellular Carcinoma,                                                                                            
          Metastatic, Liver                            1 (2%)                                                                       
      Renal Tubule, Adenoma                            1 (2%)       1 (2%)       1 (2%)                                             
   Urinary Bladder                                    (50)         (50)         (50)         (50)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
                                                                                                                                    
   Multiple Organs                                   *(50)        *(50)        *(50)        *(50)                                   
      Histiocytic Sarcoma                                                                     1 (2%)                                
      Lymphoma Malignant                               3 (6%)       3 (6%)       1 (2%)       3 (6%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  14                                                               
                                                                                                                                   
NTP Experiment-Test: 50215-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                           ACRYLONITRILE                                       Date: 10/31/00  
Route: GAVAGE                                                                                                     Time: 12:42:06  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         B6C3F1 MICE MALE                          0 MG/KG       2.5         10 MG/KG     20 MG/KG                                  
                                                                MG/KG                                                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)            45          43          49          41                                       
     Total Primary Neoplasms                           85          92         125         106                                       
                                                                                                                                    
   Total Animals with Benign Neoplasms                 34          39          45          39                                       
     Total Benign Neoplasms                            51          69          86          76                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms              29          18          30          24                                       
     Total Malignant Neoplasms                         34          23          39          30                                       
                                                                                                                                    
   Total Animals with Metastatic Neoplasms              8           7          13           9                                       
     Total Metastatic Neoplasm                         19          19          47          30                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms                                                                                           
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                                                                                              
     Total Uncertain Neoplasms                                                                                                      
                                                                                                                                    
                                                                                                                                    
____________________________________________________________________________________________________________________________________
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  15                                                               
                                                                                                                                   
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                             ----------              END OF REPORT             ----------                                           
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