TDMS Study 56348-04 Pathology Tables
NTP Experiment-Test: 56348-04 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC CITRAL Date: 07/05/01
Route: DOSED FEED Time: 13:47:25
Facility: Battelle Columbus Laboratory
Chemical CAS #: 5392-40-5
Lock Date: 03/25/99
Cage Range: All
Reasons For Removal: All
Removal Date Range: All
Treatment Groups: Include All
a Number of animals examined microscopically at site and number of animals with lesion
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NTP Experiment-Test: 56348-04 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC CITRAL Date: 07/05/01
Route: DOSED FEED Time: 13:47:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE UNTREAT VEHICLE 500 PPM 1000 PPM 2000 PPM
CONTROL CONTROL
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially in Study 50 50 50 50 50
Early Deaths
Natural Death 5 3 3 3 5
Moribund Sacrifice 4 5 2 4 5
Accidently Killed 1
Survivors
Terminal Sacrifice 40 41 45 43 40
Missing 1
Animals Examined Microscopically 50 49 50 50 50
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ALIMENTARY SYSTEM
Intestine Large, Rectum (50) (49) (50) (50) (50)
Intestine Large, Cecum (50) (49) (50) (50) (50)
Leiomyosarcoma 1 (2%)
Intestine Small, Jejunum (50) (49) (50) (50) (50)
Carcinoma 1 (2%) 1 (2%)
Intestine Small, Ileum (50) (49) (50) (50) (50)
Liver (50) (49) (50) (50) (50)
Fibrous Histiocytoma 1 (2%)
Hemangiosarcoma 1 (2%)
Hepatocellular Carcinoma 1 (2%) 2 (4%) 1 (2%) 1 (2%) 1 (2%)
Hepatocellular Carcinoma, Multiple 1 (2%)
Hepatocellular Adenoma 5 (10%) 2 (4%) 2 (4%) 8 (16%) 7 (14%)
Hepatocellular Adenoma, Multiple 1 (2%) 1 (2%)
Histiocytic Sarcoma 1 (2%)
Squamous Cell Carcinoma, Metastatic, Stomach,
Forestomach 1 (2%)
Mesentery (5) (3) (1) (6) (2)
Fibrous Histiocytoma 1 (17%)
Sarcoma, Metastatic, Skin 1 (17%)
Pancreas (50) (49) (50) (50) (50)
Salivary Glands (49) (49) (50) (50) (49)
Stomach, Forestomach (50) (49) (50) (50) (50)
Squamous Cell Carcinoma 1 (2%)
Squamous Cell Papilloma 1 (2%) 2 (4%)
Stomach, Glandular (50) (49) (50) (50) (50)
Adenoma 1 (2%)
Tongue (1) (1)
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NTP Experiment-Test: 56348-04 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC CITRAL Date: 07/05/01
Route: DOSED FEED Time: 13:47:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE UNTREAT VEHICLE 500 PPM 1000 PPM 2000 PPM
CONTROL CONTROL
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM - cont
Squamous Cell Carcinoma 1 (100%) 1 (100%)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (50) (49) (50) (50) (50)
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (50) (49) (50) (50) (50)
Adrenal Medulla (50) (49) (50) (50) (50)
Pheochromocytoma Malignant 1 (2%)
Pituitary Gland (49) (48) (50) (49) (49)
Pars Distalis, Adenoma 4 (8%) 4 (8%) 5 (10%) 2 (4%) 3 (6%)
Pars Intermedia, Adenoma 1 (2%) 1 (2%) 1 (2%)
Thyroid Gland (48) (49) (50) (50) (49)
Follicle, Adenoma 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
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GENITAL SYSTEM
Clitoral Gland (47) (48) (50) (47) (49)
Carcinoma 1 (2%)
Ovary (50) (49) (50) (49) (50)
Carcinoma, Metastatic, Stomach, Forestomach 1 (2%)
Cystadenoma 1 (2%) 2 (4%) 1 (2%) 1 (2%)
Granulosa Cell Tumor Benign 1 (2%)
Histiocytic Sarcoma 1 (2%)
Luteoma 1 (2%)
Squamous Cell Carcinoma, Metastatic, Stomach,
Forestomach 1 (2%)
Uterus (50) (49) (50) (50) (50)
Adenoma 1 (2%)
Carcinoma 1 (2%)
Leiomyosarcoma 1 (2%)
Polyp Stromal 2 (4%) 1 (2%) 1 (2%)
____________________________________________________________________________________________________________________________________
Page 3
NTP Experiment-Test: 56348-04 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC CITRAL Date: 07/05/01
Route: DOSED FEED Time: 13:47:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE UNTREAT VEHICLE 500 PPM 1000 PPM 2000 PPM
CONTROL CONTROL
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (50) (49) (50) (50) (50)
Lymph Node (2) (1) (7) (5)
Lumbar, Histiocytic Sarcoma 1 (20%)
Mediastinal, Histiocytic Sarcoma 1 (20%)
Mediastinal, Sarcoma, Metastatic, Skin 1 (14%)
Renal, Histiocytic Sarcoma 1 (20%)
Lymph Node, Mandibular (49) (49) (48) (47) (44)
Histiocytic Sarcoma 1 (2%)
Lymph Node, Mesenteric (49) (47) (48) (50) (49)
Histiocytic Sarcoma 1 (2%)
Squamous Cell Carcinoma, Metastatic, Stomach,
Forestomach 1 (2%)
Spleen (50) (49) (50) (49) (50)
Histiocytic Sarcoma 1 (2%)
Thymus (47) (48) (49) (48) (50)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Mammary Gland (50) (49) (50) (49) (49)
Carcinoma 1 (2%)
Skin (50) (49) (50) (50) (50)
Fibrous Histiocytoma 1 (2%)
Sebaceous Gland, Adenoma 1 (2%)
Subcutaneous Tissue, Hemangioma 1 (2%)
Subcutaneous Tissue, Sarcoma 2 (4%) 2 (4%) 1 (2%) 2 (4%)
Tail, Sarcoma 1 (2%)
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MUSCULOSKELETAL SYSTEM
Bone (50) (49) (50) (50) (50)
Fibrosarcoma 1 (2%)
Skeletal Muscle (1) (1) (1) (2)
Sarcoma 1 (100%)
____________________________________________________________________________________________________________________________________
Page 4
NTP Experiment-Test: 56348-04 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC CITRAL Date: 07/05/01
Route: DOSED FEED Time: 13:47:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE UNTREAT VEHICLE 500 PPM 1000 PPM 2000 PPM
CONTROL CONTROL
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (50) (48) (50) (50) (50)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (50) (49) (50) (50) (50)
Alveolar/Bronchiolar Adenoma 3 (6%) 2 (4%) 7 (14%) 3 (6%) 1 (2%)
Alveolar/Bronchiolar Carcinoma 3 (6%) 3 (6%) 1 (2%)
Carcinoma, Metastatic, Harderian Gland 1 (2%)
Fibrous Histiocytoma 1 (2%)
Hepatocellular Carcinoma, Metastatic, Liver 1 (2%)
Histiocytic Sarcoma 1 (2%)
Pheochromocytoma Malignant, Metastatic,
Adrenal Medulla 1 (2%)
Sarcoma, Metastatic, Skin 1 (2%)
Nose (50) (49) (50) (50) (50)
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SPECIAL SENSES SYSTEM
Harderian Gland (2) (3) (2) (3) (1)
Adenoma 1 (50%) 2 (67%) 1 (50%) 2 (67%) 1 (100%)
Carcinoma 1 (50%) 1 (33%)
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URINARY SYSTEM
Kidney (50) (49) (50) (50) (50)
Histiocytic Sarcoma 1 (2%)
Urinary Bladder (50) (49) (50) (50) (50)
____________________________________________________________________________________________________________________________________
SYSTEMIC LESIONS
Multiple Organs *(50) *(49) *(50) *(50) *(50)
Histiocytic Sarcoma 1 (2%)
Lymphoma Malignant 4 (8%) 3 (6%) 5 (10%) 9 (18%) 12 (24%)
____________________________________________________________________________________________________________________________________
* Number of animals with any tissue examined microscopically
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NTP Experiment-Test: 56348-04 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC CITRAL Date: 07/05/01
Route: DOSED FEED Time: 13:47:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE FEMALE UNTREAT VEHICLE 500 PPM 1000 PPM 2000 PPM
CONTROL CONTROL
____________________________________________________________________________________________________________________________________
TUMOR SUMMARY
Total Animals with Primary Neoplasms (b) 23 22 27 23 30
Total Primary Neoplasms 32 28 36 34 36
Total Animals with Benign Neoplasms 16 12 19 13 14
Total Benign Neoplasms 19 16 22 16 17
Total Animals with Malignant Neoplasms 12 12 13 13 18
Total Malignant Neoplasms 13 12 14 18 19
Total Animals with Metastatic Neoplasms 2 2 2
Total Metastatic Neoplasm 2 2 6
Total Animals with Malignant Neoplasms
Uncertain Primary Site
Total Animals with Neoplasms Uncertain-
Benign or Malignant
Total Uncertain Neoplasms
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
b Primary tumors: all tumors except metastatic tumors
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NTP Experiment-Test: 56348-04 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC CITRAL Date: 07/05/01
Route: DOSED FEED Time: 13:47:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE UNTREAT VEHICLE 500 PPM 1000 PPM 2000 PPM
CONTROL CONTROL
____________________________________________________________________________________________________________________________________
DISPOSITION SUMMARY
Animals Initially in Study 50 50 50 50 50
Early Deaths
Moribund Sacrifice 1 4 2 6 3
Natural Death 1 3 8 2 7
Survivors
Terminal Sacrifice 47 42 40 42 40
Natural Death 1
Accidently Killed 1
Animals Examined Microscopically 50 50 50 50 50
____________________________________________________________________________________________________________________________________
ALIMENTARY SYSTEM
Intestine Small, Duodenum (50) (50) (50) (49) (50)
Polyp Adenomatous 1 (2%) 1 (2%)
Intestine Small, Jejunum (50) (50) (50) (50) (50)
Carcinoma 1 (2%) 2 (4%) 1 (2%)
Intestine Small, Ileum (50) (50) (50) (50) (50)
Liver (50) (50) (50) (50) (50)
Carcinoma, Metastatic, Pancreas 1 (2%)
Hemangiosarcoma 2 (4%)
Hepatocellular Carcinoma 3 (6%) 9 (18%) 12 (24%) 4 (8%) 9 (18%)
Hepatocellular Carcinoma, Multiple 1 (2%) 1 (2%) 1 (2%)
Hepatocellular Adenoma 9 (18%) 8 (16%) 8 (16%) 11 (22%) 10 (20%)
Hepatocellular Adenoma, Multiple 1 (2%) 2 (4%) 3 (6%) 3 (6%)
Mesentery (6) (3) (2) (3) (3)
Pancreas (50) (50) (50) (50) (50)
Carcinoma 1 (2%)
Stomach, Forestomach (50) (50) (49) (50) (50)
Carcinoma, Metastatic, Pancreas 1 (2%)
Squamous Cell Papilloma 1 (2%)
Stomach, Glandular (50) (50) (50) (50) (50)
____________________________________________________________________________________________________________________________________
CARDIOVASCULAR SYSTEM
Heart (50) (50) (50) (50) (50)
Hemangiosarcoma 1 (2%)
Hepatocellular Carcinoma, Metastatic, Liver 1 (2%)
____________________________________________________________________________________________________________________________________
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NTP Experiment-Test: 56348-04 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC CITRAL Date: 07/05/01
Route: DOSED FEED Time: 13:47:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE UNTREAT VEHICLE 500 PPM 1000 PPM 2000 PPM
CONTROL CONTROL
____________________________________________________________________________________________________________________________________
ENDOCRINE SYSTEM
Adrenal Cortex (50) (50) (50) (50) (50)
Subcapsular, Adenoma 1 (2%) 1 (2%) 1 (2%)
Adrenal Medulla (49) (50) (50) (50) (50)
Pheochromocytoma Benign 1 (2%)
Islets, Pancreatic (50) (50) (50) (50) (50)
Adenoma 1 (2%)
Pituitary Gland (50) (48) (49) (47) (50)
Pars Intermedia, Adenoma 1 (2%)
Thyroid Gland (50) (50) (50) (50) (50)
Follicular Cell, Adenoma 1 (2%)
____________________________________________________________________________________________________________________________________
GENERAL BODY SYSTEM
None
____________________________________________________________________________________________________________________________________
GENITAL SYSTEM
Epididymis (50) (50) (50) (50) (50)
Carcinoma, Metastatic, Pancreas 1 (2%)
Preputial Gland (50) (50) (50) (50) (50)
Prostate (50) (50) (50) (50) (50)
Seminal Vesicle (50) (50) (50) (50) (50)
Carcinoma, Metastatic, Pancreas 1 (2%)
Testes (50) (50) (50) (50) (50)
Hemangioma 1 (2%)
Hemangiosarcoma 1 (2%)
Interstitial Cell, Adenoma 1 (2%)
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM
Bone Marrow (50) (50) (50) (50) (50)
Hemangiosarcoma 2 (4%) 1 (2%)
Mast Cell Tumor Malignant 1 (2%) 1 (2%)
Lymph Node (2) (1) (2) (1)
Mediastinal, Carcinoma, Metastatic, Pancreas 1 (50%)
Lymph Node, Mandibular (48) (45) (47) (47) (49)
Lymph Node, Mesenteric (48) (47) (49) (48) (50)
Spleen (50) (50) (49) (50) (50)
Page 8
NTP Experiment-Test: 56348-04 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC CITRAL Date: 07/05/01
Route: DOSED FEED Time: 13:47:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE UNTREAT VEHICLE 500 PPM 1000 PPM 2000 PPM
CONTROL CONTROL
____________________________________________________________________________________________________________________________________
HEMATOPOIETIC SYSTEM - cont
Hemangiosarcoma 3 (6%) 2 (4%)
Mast Cell Tumor Malignant 1 (2%)
Thymus (50) (49) (48) (48) (46)
Thymoma Benign 1 (2%)
____________________________________________________________________________________________________________________________________
INTEGUMENTARY SYSTEM
Skin (50) (50) (50) (50) (50)
Pinna, Melanoma Malignant 1 (2%)
Subcutaneous Tissue, Hemangioma 1 (2%)
Subcutaneous Tissue, Sarcoma 1 (2%)
Tail, Fibrosarcoma 1 (2%) 1 (2%)
Tail, Neurofibrosarcoma 1 (2%)
____________________________________________________________________________________________________________________________________
MUSCULOSKELETAL SYSTEM
Bone (50) (50) (50) (50) (50)
Chondrosarcoma 1 (2%)
____________________________________________________________________________________________________________________________________
NERVOUS SYSTEM
Brain (50) (50) (50) (50) (50)
Astrocytoma Benign 1 (2%)
____________________________________________________________________________________________________________________________________
RESPIRATORY SYSTEM
Lung (50) (50) (50) (50) (50)
Alveolar/Bronchiolar Adenoma 4 (8%) 8 (16%) 5 (10%) 6 (12%) 2 (4%)
Alveolar/Bronchiolar Carcinoma 4 (8%) 5 (10%) 4 (8%) 1 (2%) 3 (6%)
Carcinoma, Metastatic, Pancreas 1 (2%)
Hemangiosarcoma 1 (2%)
Hepatocellular Carcinoma, Metastatic, Liver 1 (2%) 1 (2%) 1 (2%) 2 (4%)
____________________________________________________________________________________________________________________________________
Page 9
NTP Experiment-Test: 56348-04 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC CITRAL Date: 07/05/01
Route: DOSED FEED Time: 13:47:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE UNTREAT VEHICLE 500 PPM 1000 PPM 2000 PPM
CONTROL CONTROL
____________________________________________________________________________________________________________________________________
SPECIAL SENSES SYSTEM
Harderian Gland (2) (2) (1) (3)
Adenoma 2 (100%) 2 (100%) 1 (100%) 2 (67%)
Carcinoma 1 (33%)
____________________________________________________________________________________________________________________________________
URINARY SYSTEM
Kidney (50) (50) (50) (50) (50)
Urinary Bladder (50) (50) (49) (50) (50)
Hemangioma 1 (2%)
____________________________________________________________________________________________________________________________________
SYSTEMIC LESIONS
Multiple Organs *(50) *(50) *(50) *(50) *(50)
Lymphoma Malignant 4 (8%) 2 (4%) 3 (6%) 1 (2%)
____________________________________________________________________________________________________________________________________
* Number of animals with any tissue examined microscopically
Page 10
NTP Experiment-Test: 56348-04 INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a) Report: PEIRPT05
Study Type: CHRONIC CITRAL Date: 07/05/01
Route: DOSED FEED Time: 13:47:25
____________________________________________________________________________________________________________________________________
B6C3F1 MICE MALE UNTREAT VEHICLE 500 PPM 1000 PPM 2000 PPM
CONTROL CONTROL
____________________________________________________________________________________________________________________________________
TUMOR SUMMARY
Total Animals with Primary Neoplasms (b) 27 28 35 33 26
Total Primary Neoplasms 37 43 39 44 35
Total Animals with Benign Neoplasms 20 20 18 21 14
Total Benign Neoplasms 22 23 19 23 16
Total Animals with Malignant Neoplasms 14 18 20 17 18
Total Malignant Neoplasms 15 20 20 21 19
Total Animals with Metastatic Neoplasms 1 1 2 2
Total Metastatic Neoplasm 1 2 7 2
Total Animals with Malignant Neoplasms
Uncertain Primary Site
Total Animals with Neoplasms Uncertain-
Benign or Malignant
Total Uncertain Neoplasms
____________________________________________________________________________________________________________________________________
a Number of animals examined microscopically at site and number of animals with lesion
b Primary tumors: all tumors except metastatic tumors
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