National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/7686

Statistical Analysis Of Survival Data

NTP                                         STATISTICAL ANALYSIS OF SURVIVAL DATA                              REPORT: PEIRPT11     
LAB: Battelle Columbus                            OLEIC ACID DIETHANOLAMINE CONDENSATE                         DATE: 09/23/97       
EXPERIMENT: 91014 TEST: 04                                                                                     TIME: 09:48:23       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                   PAGE:       1        
CONT: N01-ES-15314                              ROUTE: SKIN APPLICATION                                        NTP C#: C91014       
PATHOLOGIST: SELLS, D.                                                                                         CAS: 93-83-4         
------------------------------------------------------------------------------------------------------------------------------------
                                                                                                                                    
                                                           2 YEAR CHRONIC                                                           
                                                                                                                                    

               REASONS FOR REMOVAL:    25022 Accidently Killed                                                                      
                                       25023 Missing                                                                                
                                       25019 Moribund Sacrifice                                                                     
                                       25020 Natural Death                                                                          
                                       25021 Terminal Sacrifice                                                                     
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
               REMOVAL DATE RANGE:     ALL                                                                                          
                                                                                                                                    
               TREATMENT GROUPS:       INCLUDE 001    0 MG/KG                                                                       
                                       INCLUDE 002    0 MG/KG                                                                       
                                       INCLUDE 003    15 MG/KG                                                                      
                                       INCLUDE 004    15 MG/KG                                                                      
                                       INCLUDE 005    30 MG/KG                                                                      
                                       INCLUDE 006    30 MG/KG                                                                      
NTP                                         STATISTICAL ANALYSIS OF SURVIVAL DATA                              REPORT: PEIRPT11    
LAB: Battelle Columbus                            OLEIC ACID DIETHANOLAMINE CONDENSATE                         DATE: 09/23/97      
EXPERIMENT: 91014 TEST: 04                                                                                     TIME: 09:48:23      
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                       
CONT: N01-ES-15314                              ROUTE: SKIN APPLICATION                                        NTP C#: C91014      
PATHOLOGIST: SELLS, D.                                                                                         CAS: 93-83-4        
                       OLEIC ACID DIETHANOLAMINE CONDENSATE

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                     MALE MICE
                       FIRST TERMINAL SACRIFICE AT 729 DAYS
                                                          

                          INDIVIDUAL SURVIVAL TIMES (DAYS)
 ==================================================================================
 |                                DOSE = 0 MG/KG                                  |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 50     UNCENSORED DEATHS 8      CENSORED DEATHS 1    TERMINAL 41        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      452   456   485   561   667   691   695   699                             |
 |  CENSORED DEATHS                                                               |
 |      38                                                                        |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                DOSE = 15 MG/KG                                 |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 50     UNCENSORED DEATHS 15     CENSORED DEATHS 0    TERMINAL 35        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      176   425   547   567   621   624   628   628   660   686                 |
 |      697   708   709   712   720                                               |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                DOSE = 30 MG/KG                                 |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 50     UNCENSORED DEATHS 16     CENSORED DEATHS 0    TERMINAL 34        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      356   416   468   488   537   549   572   577   606   638                 |
 |      638   642   660   676   691   705                                         |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 ==================================================================================
                       OLEIC ACID DIETHANOLAMINE CONDENSATE

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                     MALE MICE
                       FIRST TERMINAL SACRIFICE AT 729 DAYS

 ==================================================================================
 |               KAPLAN-MEIER SURVIVAL PROBABILITY ESTIMATES (%)                  |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |    DOSE                               TIME (DAYS)                              |
 |                 90   180   270   365   425   485   545   605   665   729 (A)   |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |           |                                                                    |
 |0 MG/KG    |   100.0 100.0 100.0 100.0 100.0  93.9  93.9  91.8  91.8  83.7      |
 |           |                                                                    |
 |           |                                                                    |
 |15 MG/KG   |   100.0  98.0  98.0  98.0  96.0  96.0  96.0  92.0  82.0  70.0      |
 |           |                                                                    |
 |           |                                                                    |
 |30 MG/KG   |   100.0 100.0 100.0  98.0  96.0  94.0  90.0  84.0  74.0  68.0      |
 |           |                                                                    |
 |           |                                                                    |
 ==================================================================================
 ==================================================================================
 |                           SURVIVAL SUMMARY STATISTICS                          |
 |                                                                                |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                                    DOSE                        |
 |                                                                                |
 |                                0 MG/KG          15 MG/KG         30 MG/KG      |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |SURVIVAL AT END OF STUDY|       83.7%            70.0%            68.0%         |
 |(KAPLAN-MEIER)          |                                                       |
 |                        |                                                       |
 |SIGNIFICANCE (B)        |       P=0.086          P=0.182          P=0.102       |
 |(LIFE TABLE)            |                                                       |
 |                        |                                                       |
 |MEAN DAY OF             |       588.3            607.2            576.2         |
 |NATURAL DEATHS (C)      |       (39.6)           (37.0)           (25.5)        |
 |(STANDARD ERROR)        |                                                       |
 |                        |                                                       |
 |MEAN LIFE SPAN (D)      |       692.7            692.5            680.1         |
 |(STANDARD ERROR)        |       (16.4)           (13.4)           (12.9)        |
 |                        |                                                       |
 ==================================================================================
                                    

       (A)  FIRST TERMINAL SACRIFICE
       (B)  THE FIRST ENTRY IS THE TREND TEST (TARONE, 1975) RESULT.
            SUBSEQUENT ENTRIES ARE THE RESULTS OF PAIRWISE TESTS
            (COX,1972).  NEGATIVE TRENDS ARE INDICATED BY "N".
       (C)  MEAN OF ALL UNCENSORED DEATHS PRIOR TO TERMINAL SACRIFICE
       (D)  MEAN OF ALL DEATHS (UNCENSORED, CENSORED, TERMINAL SACRIFICE)
                       OLEIC ACID DIETHANOLAMINE CONDENSATE

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                    FEMALE MICE
                       FIRST TERMINAL SACRIFICE AT 730 DAYS
                                                          

                          INDIVIDUAL SURVIVAL TIMES (DAYS)
 ==================================================================================
 |                                DOSE = 0 MG/KG                                  |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 50     UNCENSORED DEATHS 16     CENSORED DEATHS 0    TERMINAL 34        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      150   435   471   562   562   578   603   615   621   654                 |
 |      660   675   682   683   719   721                                         |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                DOSE = 15 MG/KG                                 |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 50     UNCENSORED DEATHS 20     CENSORED DEATHS 0    TERMINAL 30        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      240   445   501   584   585   604   616   624   639   641                 |
 |      646   659   659   662   677   682   682   704   704   711                 |
 |  CENSORED DEATHS                                                               |
 |      NONE                                                                      |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                DOSE = 30 MG/KG                                 |
 |                             ---------------------                              |
 |                                                                                |
 |  TOTAL 50     UNCENSORED DEATHS 14     CENSORED DEATHS 1    TERMINAL 35        |
 |                                                                                |
 |  UNCENSORED DEATHS                                                             |
 |      386   478   524   527   552   604   605   637   659   669                 |
 |      673   692   721   723                                                     |
 |  CENSORED DEATHS                                                               |
 |      369                                                                       |
 |                                                                                |
 ==================================================================================
                       OLEIC ACID DIETHANOLAMINE CONDENSATE

                       STATISTICAL ANALYSIS OF SURVIVAL DATA
                                    FEMALE MICE
                       FIRST TERMINAL SACRIFICE AT 730 DAYS

 ==================================================================================
 |               KAPLAN-MEIER SURVIVAL PROBABILITY ESTIMATES (%)                  |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |    DOSE                               TIME (DAYS)                              |
 |                 90   180   270   365   425   485   545   605   665   730 (A)   |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |           |                                                                    |
 |0 MG/KG    |   100.0  98.0  98.0  98.0  98.0  94.0  94.0  86.0  78.0  68.0      |
 |           |                                                                    |
 |           |                                                                    |
 |15 MG/KG   |   100.0 100.0  98.0  98.0  98.0  96.0  94.0  88.0  72.0  60.0      |
 |           |                                                                    |
 |           |                                                                    |
 |30 MG/KG   |   100.0 100.0 100.0 100.0  98.0  95.9  91.8  85.7  81.6  71.4      |
 |           |                                                                    |
 |           |                                                                    |
 ==================================================================================
 ==================================================================================
 |                           SURVIVAL SUMMARY STATISTICS                          |
 |                                                                                |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |                                                    DOSE                        |
 |                                                                                |
 |                                0 MG/KG          15 MG/KG         30 MG/KG      |
 |                                                                                |
 |--------------------------------------------------------------------------------|
 |SURVIVAL AT END OF STUDY|       68.0%            60.0%            71.4%         |
 |(KAPLAN-MEIER)          |                                                       |
 |                        |                                                       |
 |SIGNIFICANCE (B)        |       P=0.780N         P=0.561          P=0.847N      |
 |(LIFE TABLE)            |                                                       |
 |                        |                                                       |
 |MEAN DAY OF             |       586.9            613.3            603.6         |
 |NATURAL DEATHS (C)      |       (35.5)           (24.6)           (26.4)        |
 |(STANDARD ERROR)        |                                                       |
 |                        |                                                       |
 |MEAN LIFE SPAN (D)      |       684.2            683.3            687.4         |
 |(STANDARD ERROR)        |       (14.6)           (12.7)           (12.6)        |
 |                        |                                                       |
 ==================================================================================
                                    

       (A)  FIRST TERMINAL SACRIFICE
       (B)  THE FIRST ENTRY IS THE TREND TEST (TARONE, 1975) RESULT.
            SUBSEQUENT ENTRIES ARE THE RESULTS OF PAIRWISE TESTS
            (COX,1972).  NEGATIVE TRENDS ARE INDICATED BY "N".
       (C)  MEAN OF ALL UNCENSORED DEATHS PRIOR TO TERMINAL SACRIFICE
       (D)  MEAN OF ALL DEATHS (UNCENSORED, CENSORED, TERMINAL SACRIFICE)
                                                OLEIC ACID DIETHANOLAMINE CONDENSATE
                                                    KAPLAN-MEIER SURVIVAL CURVES
                                                        0 MG/KG          = 1
                                                        15 MG/KG         = 2
                                                        30 MG/KG         = 3

------------------------------------------------------ SPECIES=MICE SEX=MALE -------------------------------------------------------

                                         Plot of SURVIVAL*WEEK.  Symbol is value of DOSE.

      |
      |
  100 + 1                            2                          3               1
      |
      |                              2                          3         32    11
      |
   96 +                                                                   32     13  1         2
      |
      |                                                                           3  1         2 1
      |
P  92 +                                                                              3       3   1        2       1
E     |
R     |                                                                                      3 3          22      1  1
C     |
E  88 +                                                                                        3  3                  11
N     |
T     |                                                                                           33
      |
S  84 +                                                                                            3    3  2     2    1     1
U     |
R     |                                                                                                 3    3   2  2
V     |
I  80 +                                                                                                             2 2
V     |
A     |                                                                                                               2 2
L     |
   76 +                                                                                                      3   3
      |
      |                                                                                                          3 3
      |
   72 +                                                                                                            3 3  22
      |
      |                                                                                                              3 3 2  2
      |
   68 +                                                                                                                3    3
      |
      --+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+--
        0         10         20         30         40         50         60         70         80         90         100        110

                                                                   WEEK

NOTE: 5 obs hidden.
                                                OLEIC ACID DIETHANOLAMINE CONDENSATE
                                                    KAPLAN-MEIER SURVIVAL CURVES
                                                        0 MG/KG          = 1
                                                        15 MG/KG         = 2
                                                        30 MG/KG         = 3

----------------------------------------------------- SPECIES=MICE SEX=FEMALE ------------------------------------------------------

                                         Plot of SURVIVAL*WEEK.  Symbol is value of DOSE.

      |
      |
  100 + 1                       1              2                      3
      |
      |                         1              2                      3      12     3
      |                                                                      12    13  2   3
   95 +
      |                                                                            1   2   33    1  2
      |
      |                                                                                     3  3
P  90 +                                                                                        3 1 12   2
E     |
R     |                                                                                            1    12
C     |                                                                                                 11 23
E  85 +
N     |                                                                                                  11232   3
T     |                                                                                                   1    1
      |                                                                                                          33
S  80 +                                                                                                      221 133
U     |
R     |                                                                                                       2  1 1 3
V     |                                                                                                            11
I  75 +                                                                                                              3   3
V     |                                                                                                                  33
A     |                                                                                                          2 21    1
L     |                                                                                                                   3 3
   70 +                                                                                                            22
      |
      |                                                                                                                  1  1
      |                                                                                                             2  2
   65 +
      |
      |                                                                                                                22
      |
   60 +                                                                                                                 2   2
      |
      --+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+--
        0         10         20         30         40         50         60         70         80         90         100        110

                                                                   WEEK

NOTE: 8 obs hidden.
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.