National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/4126

TDMS Study 93005-03 Pathology Tables

NTP Experiment-Test: 93005-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT18
Study Type: CHRONIC                                  WITH AVERAGE SEVERITY GRADES[b]                              Date: 06/18/03
Route: DOSED FEED                                              MALACHITE GREEN                                    Time: 14:30:44




       Facility:  National Center for Toxicological Research

       Chemical CAS #:  569-64-2

       Lock Date:  None

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All



































a  Number of animals examined microscopically at site and number of animals with lesion
b  Average severity grade (1-minimal;2-mild;3-moderate;4-marked)

                                                              Page   1


NTP Experiment-Test: 93005-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT18  
Study Type: CHRONIC                                  WITH AVERAGE SEVERITY GRADES[b]                              Date: 06/18/03    
Route: DOSED FEED                                              MALACHITE GREEN                                    Time: 14:30:44    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                      0 PPM        100 PPM      300 PPM      600 PPM                                
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially In Study                          48           48           48           48                                     
  Early Deaths                                                                                                                      
    Natural Death                                      1            3            1            6                                     
    Moribund Sacrifice                                18           22           15           17                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                29           23           32           25                                     
                                                                                                                                    
  Animals Examined Microscopically                    48           48           48           48                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Esophagus                                          (48)         (48)         (48)         (48)                                   
      Dilatation                                                                              1 [3.0]                               
      Foreign Body                                                                            1                                     
   Intestine Small, Ileum                             (47)         (46)         (48)         (47)                                   
      Dilatation                                                    1 [3.0]                                                         
   Liver                                              (48)         (48)         (48)         (48)                                   
      Angiectasis                                      1 [2.0]                                                                      
      Basophilic Focus                                 2            3            2            7                                     
      Basophilic Focus, Multiple                      19           14           18            8                                     
      Clear Cell Focus                                              2                                                               
      Clear Cell Focus, Multiple                                                 1                                                  
      Cyst                                                          1 [2.0]                                                         
      Degeneration, Cystic                                                       1 [2.0]                                            
      Developmental Malformation                       4 [0.0]      7 [0.0]      2 [0.0]      5 [1.0]                               
      Eosinophilic Focus                               3            9            3           11                                     
      Eosinophilic Focus, Multiple                     2            1           10            3                                     
      Fatty Change                                                                            1 [2.0]                               
      Hematopoietic Cell Proliferation                 2 [1.5]      3 [1.0]      4 [1.0]      5 [1.8]                               
      Hepatodiaphragmatic Nodule                       3            6            2            3                                     
      Infiltration Cellular, Lymphocyte                2 [1.0]      3 [1.3]      3 [1.0]      8 [1.0]                               
      Inflammation, Focal, Granulomatous              18 [1.6]     15 [1.5]     23 [1.5]     19 [1.3]                               
      Inflammation, Focal, Suppurative                 1 [1.0]      2 [2.5]                                                         
      Mixed Cell Focus                                              2                                                               
      Mixed Cell Focus, Multiple                                                              1                                     
      Necrosis                                                      2 [1.5]      2 [1.5]      1 [1.0]                               
      Vacuolization Cytoplasmic                        4 [2.3]      4 [2.8]      1 [3.0]      6 [1.8]                               
      Bile Duct, Cyst                                                            1 [2.0]                                            
      Bile Duct, Hyperplasia                          10 [1.4]     10 [1.7]      9 [1.4]      5 [1.2]                               
      Centrilobular, Necrosis                                                                 4 [2.5]                               
   Mesentery                                          (9)          (5)          (2)          (4)                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Average severity grade (1-minimal;2-mild;3-moderate;4-marked)                                                                    
                                                                                                                                    
                                                             Page    2                                                              
                                                                                                                                   
NTP Experiment-Test: 93005-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT18  
Study Type: CHRONIC                                  WITH AVERAGE SEVERITY GRADES[b]                              Date: 06/18/03    
Route: DOSED FEED                                              MALACHITE GREEN                                    Time: 14:30:44    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                      0 PPM        100 PPM      300 PPM      600 PPM                                
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ALIMENTARY SYSTEM - CONT                                                                                                            
      Fat, Necrosis                                    7 [2.6]      4 [3.0]      2 [2.5]      3 [2.7]                               
   Pancreas                                           (48)         (48)         (48)         (48)                                   
      Infiltration Cellular, Lymphocyte                                          1 [1.0]      2 [1.0]                               
      Inflammation                                                                            1 [2.0]                               
      Acinus, Atrophy                                 13 [1.2]     14 [1.6]     12 [1.3]      7 [1.1]                               
   Salivary Glands                                    (48)         (47)         (48)         (48)                                   
      Atrophy                                                       2 [1.5]      1 [1.0]      1 [2.0]                               
      Infiltration Cellular, Lymphocyte                1 [1.0]                                1 [1.0]                               
   Stomach, Forestomach                               (48)         (48)         (48)         (48)                                   
      Edema                                                         1 [2.0]                                                         
      Erosion                                                                    1 [2.0]                                            
      Inflammation                                     1 [3.0]      2 [2.0]      1 [3.0]                                            
      Necrosis                                                      1 [2.0]                                                         
      Epithelium, Hyperplasia                                       1 [2.0]                   1 [1.0]                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (48)         (48)         (48)         (48)                                   
      Cardiomyopathy                                  13 [1.5]     22 [1.1]     19 [1.1]     14 [1.2]                               
      Atrium, Thrombosis                               1 [2.0]                                                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (48)         (48)         (48)         (48)                                   
      Accessory Adrenal Cortical Nodule                1 [2.0]                   1 [2.0]                                            
      Angiectasis                                     10 [1.3]     11 [1.5]     17 [1.5]     16 [1.6]                               
      Atrophy                                                                                 2 [2.0]                               
      Hematopoietic Cell Proliferation                                                        1 [1.0]                               
      Hyperplasia, Focal                                            1 [2.0]      1 [2.0]      1 [2.0]                               
      Hypertrophy, Focal                               1 [2.0]      4 [1.5]      1 [2.0]      4 [1.8]                               
      Infiltration Cellular, Lymphocyte                                                       1 [1.0]                               
      Vacuolization Cytoplasmic                        6 [1.3]      5 [1.8]      2 [1.5]      3 [1.3]                               
   Adrenal Medulla                                    (47)         (47)         (48)         (48)                                   
      Hyperplasia                                      1 [1.0]      3 [2.3]      1 [3.0]      1 [1.0]                               
   Islets, Pancreatic                                 (48)         (48)         (48)         (48)                                   
      Hyperplasia                                      1 [2.0]                                                                      
   Parathyroid Gland                                  (43)         (43)         (45)         (39)                                   
      Hyperplasia, Focal                               1 [2.0]                                                                      
   Pituitary Gland                                    (48)         (47)         (46)         (45)                                   
      Angiectasis                                      5 [2.4]      2 [1.5]      2 [2.0]      2 [2.0]                               
      Pars Distalis, Cyst                              8 [1.5]      5 [1.4]      9 [1.9]      9 [1.9]                               
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Average severity grade (1-minimal;2-mild;3-moderate;4-marked)                                                                    
                                                                                                                                    
                                                             Page    3                                                              
                                                                                                                                   
NTP Experiment-Test: 93005-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT18  
Study Type: CHRONIC                                  WITH AVERAGE SEVERITY GRADES[b]                              Date: 06/18/03    
Route: DOSED FEED                                              MALACHITE GREEN                                    Time: 14:30:44    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                      0 PPM        100 PPM      300 PPM      600 PPM                                
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ENDOCRINE SYSTEM - CONT                                                                                                             
      Pars Distalis, Hyperplasia                       5 [2.0]                  11 [2.2]      6 [1.8]                               
      Pars Intermedia, Cyst                                                                   1 [2.0]                               
   Thyroid Gland                                      (46)         (48)         (47)         (46)                                   
      C-Cell, Hyperplasia                              8 [1.4]      5 [1.4]     11 [1.4]      6 [1.7]                               
      Follicle, Cyst                                                1 [3.0]      1 [3.0]      3 [2.0]                               
      Follicular Cell, Hyperplasia                                               1 [1.0]      2 [1.0]                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Clitoral Gland                                     (48)         (48)         (47)         (47)                                   
      Hyperplasia                                      5 [2.2]      4 [2.0]      2 [2.0]      6 [2.2]                               
      Inflammation                                    21 [2.1]     30 [2.2]     23 [2.2]     26 [2.2]                               
      Metaplasia, Squamous                                                                    1 [2.0]                               
      Duct, Dilatation                                 6 [2.8]      5 [2.8]      7 [2.6]      6 [2.7]                               
   Ovary                                              (48)         (48)         (47)         (48)                                   
      Atrophy                                         18 [2.4]     14 [2.1]     11 [2.5]     10 [2.0]                               
      Congestion                                                                              1 [3.0]                               
      Cyst                                             5 [2.4]      4 [2.3]      7 [2.0]     10 [2.1]                               
      Cyst, Multiple                                                                          1 [2.0]                               
      Infiltration Cellular, Lymphocyte                1 [3.0]                                                                      
      Interstitial Cell, Hyperplasia                                1 [3.0]                                                         
   Uterus                                             (48)         (48)         (48)         (48)                                   
      Decidual Reaction                                                                       1 [2.0]                               
      Hemorrhage                                                                              1 [4.0]                               
      Inflammation, Suppurative                        1 [2.0]                                                                      
      Thrombosis                                                                 1 [3.0]      1 [3.0]                               
      Cervix, Hypertrophy                              1 [3.0]      1 [3.0]      1 [2.0]                                            
      Cervix, Serosa, Cyst                             1 [3.0]                                                                      
      Endometrium, Hyperplasia, Cystic                 6 [2.0]      3 [1.7]     11 [1.8]      8 [1.8]                               
   Vagina                                             (48)         (48)         (48)         (48)                                   
      Inflammation, Suppurative                        3 [2.0]      3 [2.3]      5 [2.0]      1 [2.0]                               
      Prolapse                                                                                2 [3.0]                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (48)         (48)         (48)         (48)                                   
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Average severity grade (1-minimal;2-mild;3-moderate;4-marked)                                                                    
                                                                                                                                    
                                                             Page    4                                                              
                                                                                                                                   
NTP Experiment-Test: 93005-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT18  
Study Type: CHRONIC                                  WITH AVERAGE SEVERITY GRADES[b]                              Date: 06/18/03    
Route: DOSED FEED                                              MALACHITE GREEN                                    Time: 14:30:44    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                      0 PPM        100 PPM      300 PPM      600 PPM                                
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - CONT                                                                                                         
      Atrophy                                          1 [2.0]      3 [1.7]      2 [2.0]      2 [2.0]                               
      Myelofibrosis                                                              2 [2.0]                                            
      Myelostromal Proliferation                       1 [2.0]                                                                      
      Myeloid Cell, Hyperplasia                        5 [2.0]                   5 [2.0]      4 [2.3]                               
   Lymph Node                                         (10)         (7)          (7)          (5)                                    
      Pigmentation                                                  1 [2.0]                                                         
      Deep Cervical, Hemorrhage                        1 [2.0]                                                                      
      Deep Cervical, Hyperplasia, Lymphoid             1 [2.0]                                1 [2.0]                               
      Iliac, Hyperplasia, Lymphoid                     1 [2.0]                                                                      
      Lumbar, Ectasia                                                                         2 [3.0]                               
      Lumbar, Hematopoietic Cell Proliferation                      1 [2.0]                                                         
      Lumbar, Hemorrhage                                            1 [2.0]                   1 [1.0]                               
      Lumbar, Hyperplasia, Lymphoid                    2 [2.0]                                1 [4.0]                               
      Mediastinal, Hemorrhage                          1 [2.0]      1 [2.0]      3 [2.3]                                            
      Mediastinal, Hyperplasia, Lymphoid               2 [2.0]                                                                      
      Pancreatic, Hematopoietic Cell Proliferation                               1 [3.0]                                            
      Pancreatic, Hemorrhage                           1 [2.0]                                                                      
      Pancreatic, Hyperplasia, Lymphoid                1 [2.0]                                1 [2.0]                               
      Renal, Ectasia                                   1 [3.0]                                                                      
      Renal, Hemorrhage                                2 [2.0]                   1 [1.0]                                            
      Renal, Hyperplasia, Lymphoid                                  1 [2.0]      1 [2.0]                                            
   Lymph Node, Mandibular                             (47)         (46)         (47)         (48)                                   
      Ectasia                                                       1 [2.0]                                                         
      Hemorrhage                                                                 2 [2.5]                                            
      Hyperplasia, Lymphoid                            1 [2.0]      3 [2.0]      2 [2.0]      5 [2.6]                               
      Infiltration Cellular, Plasma Cell               5 [2.2]      5 [2.0]      5 [2.2]      7 [2.0]                               
   Lymph Node, Mesenteric                             (47)         (47)         (48)         (48)                                   
      Ectasia                                                                    1 [2.0]                                            
      Hemorrhage                                                    1 [2.0]      1 [2.0]                                            
      Hyperplasia, Lymphoid                            2 [2.0]                                                                      
      Infiltration Cellular, Plasma Cell                            1 [3.0]                   1 [2.0]                               
   Spleen                                             (48)         (48)         (48)         (48)                                   
      Atrophy                                                                                 1 [3.0]                               
      Fibrosis                                                                                1 [3.0]                               
      Hematopoietic Cell Proliferation                 6 [2.8]      2 [2.0]      7 [2.3]     12 [2.2]                               
      Hyperplasia, Focal                               1 [4.0]                                                                      
      Pigmentation                                     1 [3.0]      2 [3.0]      2 [3.0]      3 [3.0]                               
      Thrombosis                                                                 1 [3.0]                                            
      Capsule, Hemorrhage                                           1 [3.0]                                                         
   Thymus                                             (40)         (42)         (43)         (40)                                   
      Cyst                                             1 [2.0]                                                                      
      Ectopic Parathyroid Gland                                     1 [2.0]                                                         
      Hemorrhage                                                                 1 [2.0]                                            
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Average severity grade (1-minimal;2-mild;3-moderate;4-marked)                                                                    
                                                                                                                                    
                                                             Page    5                                                              
                                                                                                                                   
NTP Experiment-Test: 93005-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT18  
Study Type: CHRONIC                                  WITH AVERAGE SEVERITY GRADES[b]                              Date: 06/18/03    
Route: DOSED FEED                                              MALACHITE GREEN                                    Time: 14:30:44    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                      0 PPM        100 PPM      300 PPM      600 PPM                                
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
HEMATOPOIETIC SYSTEM - CONT                                                                                                         
      Hyperplasia, Reticulum Cell                                                             1 [3.0]                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (46)         (48)         (48)         (48)                                   
      Galactocele                                      4 [2.5]      8 [3.0]      2 [2.5]      2 [2.5]                               
      Hyperplasia                                     28 [2.1]     33 [1.8]     29 [1.9]     35 [2.0]                               
      Inflammation                                                  1 [3.0]                                                         
      Duct, Dilatation                                12 [2.0]     22 [1.9]     18 [1.9]     11 [2.1]                               
   Skin                                               (48)         (48)         (48)         (48)                                   
      Hyperkeratosis                                   2 [2.5]                                                                      
      Inflammation                                                  1 [3.0]                                                         
      Epidermis, Hyperplasia                                        1 [2.0]                                                         
      Epidermis, Necrosis                                           2 [2.0]                                                         
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (48)         (48)         (48)         (48)                                   
      Femur, Fibrous Osteodystrophy                    1 [3.0]      1 [2.0]                   3 [1.0]                               
      Femur, Osteopetrosis                             8 [2.6]      3 [2.0]      3 [2.7]      3 [2.3]                               
      Sternum, Fibrous Osteodystrophy                               1 [2.0]                                                         
      Sternum, Osteopetrosis                           8 [2.6]      3 [2.0]      3 [2.7]      3 [2.3]                               
      Turbinate, Osteopetrosis                         7 [2.7]      2 [2.5]      2 [3.0]      2 [2.5]                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (48)         (48)         (48)         (48)                                   
      Hemorrhage                                       1 [1.0]      1 [2.0]                                                         
      Hypothalamus, Compression                        5 [1.8]     14 [2.0]     10 [2.3]      7 [2.3]                               
   Spinal Cord                                        (48)         (48)         (48)         (48)                                   
      Hemorrhage                                       1 [1.0]                                                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (48)         (48)         (48)         (48)                                   
      Foreign Body                                                               1 [2.0]      4 [2.8]                               
      Hemorrhage                                                                              2 [1.5]                               
      Inflammation, Chronic, Diffuse                                1 [2.0]                                                         
      Inflammation, Chronic, Focal                     1 [2.0]      3 [1.7]      4 [1.8]      8 [1.9]                               
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Average severity grade (1-minimal;2-mild;3-moderate;4-marked)                                                                    
                                                                                                                                    
                                                             Page    6                                                              
                                                                                                                                   
NTP Experiment-Test: 93005-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT18  
Study Type: CHRONIC                                  WITH AVERAGE SEVERITY GRADES[b]                              Date: 06/18/03    
Route: DOSED FEED                                              MALACHITE GREEN                                    Time: 14:30:44    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                      0 PPM        100 PPM      300 PPM      600 PPM                                
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
RESPIRATORY SYSTEM - CONT                                                                                                           
      Alveolar Epithelium, Hyperplasia, Focal          1 [2.0]      1 [2.0]      2 [3.0]                                            
      Alveolus, Infiltration Cellular, Histiocyte      1 [1.0]      1 [2.0]                                                         
   Nose                                               (48)         (48)         (48)         (48)                                   
      Foreign Body                                                               1 [2.0]      2 [3.0]                               
      Inflammation, Chronic                            1 [2.0]                                                                      
      Inflammation, Suppurative                                                  1 [3.0]      2 [3.0]                               
      Nasolacrimal Duct, Inflammation                 19 [1.7]     10 [1.6]     11 [1.7]     13 [1.7]                               
      Olfactory Epithelium, Cytoplasmic Alteration     2 [2.5]      3 [2.3]      6 [1.8]      1 [1.0]                               
      Olfactory Epithelium, Respiratory Epithelium,                                                                                 
         Cytoplasmic Alteration                        1 [2.0]                   2 [3.0]                                            
      Respiratory Epithelium, Cytoplasmic                                                                                           
        Alteration                                                                            1 [1.0]                               
   Trachea                                            (48)         (48)         (48)         (48)                                   
      Inflammation                                                               3 [1.7]      1 [3.0]                               
      Epithelium, Hyperplasia                                                    1 [2.0]      2 [2.5]                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                (47)         (48)         (48)         (48)                                   
      Cataract                                         7 [1.4]      2 [1.5]                   7 [1.9]                               
      Hemorrhage                                                                 1 [2.0]                                            
      Phthisis Bulbi                                                             2                                                  
      Bilateral, Cataract                              1 [1.0]      1 [2.0]      2 [2.0]      2 [2.0]                               
      Bilateral, Retina, Degeneration                 13 [2.8]     14 [2.1]     20 [2.2]     18 [2.3]                               
      Cornea, Inflammation                                                                    1 [3.0]                               
      Retina, Autolysis                                1            4                         2                                     
      Retina, Degeneration                             8 [2.1]      8 [2.0]      9 [1.9]      9 [2.1]                               
      Sclera, Metaplasia, Osseous                      1 [2.0]                                                                      
   Harderian Gland                                    (47)         (48)         (48)         (48)                                   
      Atrophy                                          1 [1.0]                                                                      
      Infiltration Cellular, Lymphocyte               34 [1.4]     37 [1.2]     36 [1.3]     32 [1.4]                               
      Inflammation                                     1 [3.0]                                                                      
      Pigmentation                                                                            1 [3.0]                               
   Lacrimal Gland                                     (18)         (14)         (15)         (9)                                    
      Infiltration Cellular, Lymphocyte                2 [1.5]      2 [1.0]      2 [1.5]      3 [1.0]                               
      Metaplasia                                      18 [2.0]     14 [1.9]     15 [2.0]      9 [2.1]                               
   Zymbal's Gland                                     (47)         (48)         (48)         (46)                                   
      Hyperplasia                                      1 [2.0]      1 [2.0]                                                         
      Metaplasia, Squamous                                          1 [1.0]                                                         
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Average severity grade (1-minimal;2-mild;3-moderate;4-marked)                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page    7                                                              
                                                                                                                                   
NTP Experiment-Test: 93005-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT18  
Study Type: CHRONIC                                  WITH AVERAGE SEVERITY GRADES[b]                              Date: 06/18/03    
Route: DOSED FEED                                              MALACHITE GREEN                                    Time: 14:30:44    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS FEMALE                      0 PPM        100 PPM      300 PPM      600 PPM                                
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (48)         (48)         (48)         (48)                                   
      Hydronephrosis                                                             1 [2.0]                                            
      Mineralization                                  33 [2.0]     31 [2.0]     30 [1.9]     30 [1.8]                               
      Nephropathy                                     39 [1.4]     38 [1.6]     41 [1.4]     40 [1.5]                               
      Pelvis, Inflammation                                          1 [1.0]                                                         
      Pelvis, Transitional Epithelium, Hyperplasia                  1 [1.0]                                                         
      Renal Tubule, Necrosis                                                                  1 [3.0]                               
   Urinary Bladder                                    (47)         (48)         (47)         (47)                                   
      Infiltration Cellular, Lymphocyte                1 [1.0]                   1 [1.0]      1 [1.0]                               
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Average severity grade (1-minimal;2-mild;3-moderate;4-marked)                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
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NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.