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TDMS Study 93029-03 Pathology Tables

NTP Experiment-Test: 93029-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                        LEUKOMALACHITE GREEN                                   Date: 06/18/03
Route: DOSED FEED                                                                                                 Time: 15:01:05




       Facility:  National Center for Toxicological Research

       Chemical CAS #:  129-73-7

       Lock Date:  None

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All




































a  Number of animals examined microscopically at site and number of animals with lesion

                                                              Page   1


NTP Experiment-Test: 93029-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                        LEUKOMALACHITE GREEN                                   Date: 06/18/03  
Route: DOSED FEED                                                                                                 Time: 15:01:05  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS-NCTR FEMALE              0 PPM        91 PPM       272 PPM      543 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially in Study                          48           48           48           48                                     
  Early Deaths                                                                                                                      
    Moribund                                          12           11           12           10                                     
    Natural Death                                      3            1            1            4                                     
  Survivors                                                                                                                         
    Moribund                                                                                  1                                     
    Terminal Sacrifice                                33           36           35           33                                     
                                                                                                                                    
  Animals Examined Microscopically                    48           48           48           48                                     
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Intestine Large                                    (48)         (48)         (48)         (48)                                   
      Colon, Adenocarcinoma                                                                   1 (2%)                                
   Intestine Small                                    (48)         (48)         (48)         (48)                                   
   Liver                                              (48)         (48)         (48)         (48)                                   
      Hepatocellular Adenoma                           1 (2%)       3 (6%)                    3 (6%)                                
   Mesentery                                          (8)          (7)          (1)          (4)                                    
      Sarcoma                                          1 (13%)                                                                      
      Schwannoma Malignant, Metastatic, Uterus         1 (13%)                                                                      
   Pancreas                                           (48)         (48)         (47)         (48)                                   
      Schwannoma Malignant, Metastatic, Uterus         1 (2%)                                                                       
      Acinar Cell, Adenoma                             1 (2%)                                                                       
   Salivary Glands                                    (48)         (48)         (48)         (48)                                   
      Sarcoma                                                                    1 (2%)                                             
      Parotid Gland, Adenoma                                                                  1 (2%)                                
   Stomach                                            (48)         (48)         (48)         (48)                                   
   Tongue                                             (48)         (48)         (48)         (48)                                   
      Squamous Cell Carcinoma                                                    1 (2%)                                             
      Squamous Cell Papilloma                                                                 1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Blood Vessel                                       (48)         (48)         (48)         (48)                                   
   Heart                                              (48)         (48)         (48)         (48)                                   
      Endocardium, Schwannoma Malignant                                          1 (2%)                                             
      Myocardium, Schwannoma Malignant                                           1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   2                                                               
                                                                                                                                   
NTP Experiment-Test: 93029-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                        LEUKOMALACHITE GREEN                                   Date: 06/18/03  
Route: DOSED FEED                                                                                                 Time: 15:01:05  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS-NCTR FEMALE              0 PPM        91 PPM       272 PPM      543 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Gland                                      (47)         (48)         (47)         (48)                                   
      Medulla, Pheochromocytoma Malignant                                        1 (2%)                                             
      Medulla, Pheochromocytoma Benign                                           1 (2%)                                             
   Islets, Pancreatic                                 (48)         (48)         (47)         (48)                                   
   Pituitary Gland                                    (47)         (47)         (45)         (46)                                   
      Pars Distalis, Adenoma                          26 (55%)     23 (49%)     17 (38%)     20 (43%)                               
   Thyroid Gland                                      (46)         (46)         (47)         (48)                                   
      Bilateral, C-Cell, Adenoma                                                 1 (2%)       1 (2%)                                
      Bilateral, C-Cell, Carcinoma                                               1 (2%)                                             
      C-Cell, Adenoma                                  4 (9%)       7 (15%)      4 (9%)       4 (8%)                                
      C-Cell, Carcinoma                                             1 (2%)                                                          
      Follicular Cell, Adenoma                                                                1 (2%)                                
      Follicular Cell, Carcinoma                                    1 (2%)       2 (4%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   Tissue NOS                                         (1)                       (1)                                                 
      Schwannoma Malignant, Metastatic, Uterus         1 (100%)                                                                     
      Abdominal, Lipoma                                                          1 (100%)                                           
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Clitoral Gland                                     (47)         (47)         (46)         (47)                                   
      Adenoma                                          7 (15%)      8 (17%)      3 (7%)       6 (13%)                               
      Carcinoma                                        2 (4%)       1 (2%)       2 (4%)       2 (4%)                                
   Ovary                                              (48)         (48)         (48)         (48)                                   
   Oviduct                                            (1)                                                                           
      Leiomyoma                                        1 (100%)                                                                     
   Uterus                                             (48)         (48)         (48)         (48)                                   
      Leiomyoma                                        1 (2%)                                 1 (2%)                                
      Polyp Stromal                                    9 (19%)     10 (21%)     16 (33%)     16 (33%)                               
      Sarcoma Stromal                                  1 (2%)                                 1 (2%)                                
      Schwannoma Malignant                             1 (2%)                    1 (2%)                                             
      Bilateral, Polyp Stromal                         1 (2%)       2 (4%)       3 (6%)       1 (2%)                                
      Cervix, Squamous Cell Papilloma                               1 (2%)                                                          
      Endometrium, Adenoma                                          1 (2%)                    1 (2%)                                
      Endometrium, Carcinoma                                        1 (2%)                                                          
   Vagina                                             (48)         (48)         (48)         (48)                                   
      Sarcoma                                                                    1 (2%)                                             
      Schwannoma Malignant                                          1 (2%)                                                          
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   3                                                               
                                                                                                                                   
NTP Experiment-Test: 93029-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                        LEUKOMALACHITE GREEN                                   Date: 06/18/03  
Route: DOSED FEED                                                                                                 Time: 15:01:05  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS-NCTR FEMALE              0 PPM        91 PPM       272 PPM      543 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Lymph Node                                         (48)         (48)         (48)         (48)                                   
      Mesenteric, Schwannoma Malignant, Metastatic,                                                                                 
           Uterus                                      1 (2%)                                                                       
   Spleen                                             (48)         (48)         (48)         (48)                                   
   Thymus                                             (41)         (47)         (44)         (42)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (48)         (46)         (48)         (48)                                   
      Adenoma                                                       1 (2%)       1 (2%)       2 (4%)                                
      Carcinoma                                                     1 (2%)       2 (4%)       2 (4%)                                
      Fibroadenoma                                    20 (42%)     12 (26%)      9 (19%)     13 (27%)                               
      Fibroadenoma, Multiple                                                     3 (6%)                                             
   Skin                                               (48)         (48)         (48)         (48)                                   
      Basal Cell Adenoma                               1 (2%)       1 (2%)                                                          
      Basal Cell Carcinoma                                                                    1 (2%)                                
      Fibroma                                                       1 (2%)                    1 (2%)                                
      Fibrous Histiocytoma                                                       1 (2%)                                             
      Keratoacanthoma                                               1 (2%)                    1 (2%)                                
      Sarcoma                                                       2 (4%)                                                          
      Squamous Cell Papilloma                          1 (2%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (48)         (48)         (48)         (48)                                   
      Cranial Nerve, Schwannoma Malignant                                        1 (2%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   4                                                               
                                                                                                                                   
NTP Experiment-Test: 93029-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                        LEUKOMALACHITE GREEN                                   Date: 06/18/03  
Route: DOSED FEED                                                                                                 Time: 15:01:05  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS-NCTR FEMALE              0 PPM        91 PPM       272 PPM      543 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (48)         (48)         (48)         (48)                                   
      Alveolar/Bronchiolar Adenoma                     1 (2%)       2 (4%)       3 (6%)                                             
      Carcinoma, Metastatic, Thyroid Gland                                       1 (2%)                                             
   Nose                                               (48)         (48)         (48)         (48)                                   
   Trachea                                            (48)         (48)         (48)         (48)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Ear                                                             (1)                                                              
      Pinna, Neural Crest Tumor                                     1 (100%)                                                        
   Eye                                                (48)         (48)         (48)         (48)                                   
      Lids, Sarcoma                                    1 (2%)                                                                       
   Harderian Gland                                    (48)         (48)         (48)         (48)                                   
   Zymbal's Gland                                     (46)         (47)         (47)         (46)                                   
      Adenoma                                                                                 2 (4%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (48)         (48)         (48)         (48)                                   
      Hemangiosarcoma                                               1 (2%)                                                          
      Mesenchymal Tumor Benign                                                   1 (2%)                                             
      Sarcoma                                          1 (2%)                                                                       
      Renal Tubule, Carcinoma                          1 (2%)                                                                       
   Urinary Bladder                                    (47)         (47)         (48)         (48)                                   
      Transitional Epithelium, Carcinoma                            1 (2%)                                                          
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
                                                                                                                                    
   Multiple Organs                                   *(48)        *(48)        *(48)        *(48)                                   
      Hemangiosarcoma                                               1 (2%)                                                          
      Leukemia Mononuclear                            17 (35%)      8 (17%)      5 (10%)      8 (17%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   5                                                               
                                                                                                                                   
NTP Experiment-Test: 93029-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                        LEUKOMALACHITE GREEN                                   Date: 06/18/03  
Route: DOSED FEED                                                                                                 Time: 15:01:05  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS-NCTR FEMALE              0 PPM        91 PPM       272 PPM      543 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)            46          46          42          43                                       
     Total Primary Neoplasms                           99          92          84          90                                       
                                                                                                                                    
   Total Animals with Benign Neoplasms                 41          43          37          38                                       
     Total Benign Neoplasms                            74          73          63          75                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms              21          16          19          15                                       
     Total Malignant Neoplasms                         25          18          21          15                                       
                                                                                                                                    
   Total Animals with Metastatic Neoplasms              1                       1                                                   
     Total Metastatic Neoplasm                          4                       1                                                   
                                                                                                                                    
   Total Animals with Malignant Neoplasms                                                                                           
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                              1                                                               
     Total Uncertain Neoplasms                                      1                                                               
                                                                                                                                    
                                                                                                                                    
____________________________________________________________________________________________________________________________________
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   6                                                               
                                                                                                                                   
NTP Experiment-Test: 93029-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                        LEUKOMALACHITE GREEN                                   Date: 06/18/03  
Route: DOSED FEED                                                                                                 Time: 15:01:05  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS-NCTR MALE                0 PPM        91 PPM       272 PPM      543 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially in Study                          48           48           48           48                                     
  Early Deaths                                                                                                                      
    Moribund                                          20           12           11           10                                     
    Natural Death                                      4            5            3            7                                     
  Survivors                                                                                                                         
    Natural Death                                      1            1                                                               
    Terminal Sacrifice                                23           29           34           30                                     
  Wrong Sex                                                         1                         1                                     
                                                                                                                                    
  Animals Examined Microscopically                    48           47           48           47                                     
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Intestine Large                                    (48)         (47)         (48)         (47)                                   
      Colon, Adenoma                                                             1 (2%)                                             
      Rectum, Sarcoma, Metastatic, Skin                             1 (2%)                                                          
   Intestine Small                                    (47)         (47)         (48)         (47)                                   
      Ileum, Leiomyoma                                 1 (2%)       1 (2%)                                                          
      Jejunum, Leiomyoma                                                                      1 (2%)                                
   Liver                                              (48)         (47)         (48)         (47)                                   
      Fibrous Histiocytoma, Metastatic, Skin                                                  1 (2%)                                
      Hepatocellular Adenoma                           2 (4%)       2 (4%)       3 (6%)       2 (4%)                                
      Histiocytic Sarcoma                              1 (2%)                                                                       
      Sarcoma, Metastatic, Skin                                     1 (2%)                                                          
   Mesentery                                          (5)          (3)          (6)          (3)                                    
   Pancreas                                           (48)         (47)         (48)         (47)                                   
      Sarcoma, Metastatic, Skin                                     1 (2%)                                                          
      Acinar Cell, Adenocarcinoma                                                             1 (2%)                                
   Salivary Glands                                    (48)         (47)         (48)         (47)                                   
      Fibrous Histiocytoma                                                                    1 (2%)                                
      Histiocytic Sarcoma                                           1 (2%)                                                          
   Stomach                                            (48)         (47)         (47)         (47)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Blood Vessel                                       (48)         (47)         (48)         (47)                                   
   Heart                                              (48)         (47)         (48)         (47)                                   
      Histiocytic Sarcoma                                           1 (2%)                                                          
      Sarcoma, Metastatic, Skin                                                               1 (2%)                                
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   7                                                               
                                                                                                                                   
NTP Experiment-Test: 93029-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                        LEUKOMALACHITE GREEN                                   Date: 06/18/03  
Route: DOSED FEED                                                                                                 Time: 15:01:05  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS-NCTR MALE                0 PPM        91 PPM       272 PPM      543 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Gland                                      (48)         (47)         (48)         (47)                                   
      Bilateral, Medulla, Pheochromocytoma Benign                   1 (2%)                                                          
      Cortex, Adenoma                                  1 (2%)       1 (2%)       1 (2%)                                             
      Medulla, Pheochromocytoma Malignant                           2 (4%)                    3 (6%)                                
      Medulla, Pheochromocytoma Complex                                                       1 (2%)                                
      Medulla, Pheochromocytoma Benign                 3 (6%)       5 (11%)      2 (4%)       3 (6%)                                
   Islets, Pancreatic                                 (48)         (47)         (48)         (47)                                   
      Adenoma                                          5 (10%)      2 (4%)       1 (2%)       4 (9%)                                
   Pituitary Gland                                    (45)         (46)         (48)         (45)                                   
      Craniopharyngioma                                                                       1 (2%)                                
      Pars Distalis, Adenoma                          30 (67%)     19 (41%)     21 (44%)     13 (29%)                               
   Thyroid Gland                                      (47)         (47)         (48)         (46)                                   
      Fibrous Histiocytoma                                                                    1 (2%)                                
      Bilateral, Follicular Cell, Carcinoma                                                   1 (2%)                                
      C-Cell, Adenoma                                  6 (13%)      7 (15%)      7 (15%)      4 (9%)                                
      C-Cell, Carcinoma                                1 (2%)       2 (4%)                                                          
      Follicular Cell, Adenoma                                      2 (4%)                    1 (2%)                                
      Follicular Cell, Carcinoma                                                 1 (2%)       1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   Tissue NOS                                         (1)                                    (1)                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Epididymis                                         (48)         (47)         (48)         (47)                                   
      Sarcoma, Metastatic, Skin                                     2 (4%)                                                          
   Preputial Gland                                    (48)         (47)         (48)         (47)                                   
      Adenoma                                          5 (10%)      2 (4%)       7 (15%)      3 (6%)                                
      Carcinoma                                        6 (13%)                   4 (8%)       9 (19%)                               
      Sarcoma, Metastatic, Skin                                     2 (4%)                                                          
      Squamous Cell Papilloma                                                                 1 (2%)                                
   Prostate                                           (48)         (47)         (48)         (47)                                   
   Testes                                             (48)         (47)         (48)         (47)                                   
      Bilateral, Interstitial Cell, Adenoma           22 (46%)     30 (64%)     38 (79%)     39 (83%)                               
      Interstitial Cell, Adenoma                      15 (31%)     12 (26%)      5 (10%)      6 (13%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   8                                                               
                                                                                                                                   
NTP Experiment-Test: 93029-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                        LEUKOMALACHITE GREEN                                   Date: 06/18/03  
Route: DOSED FEED                                                                                                 Time: 15:01:05  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS-NCTR MALE                0 PPM        91 PPM       272 PPM      543 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Bone Marrow                                        (48)         (47)         (48)         (47)                                   
   Lymph Node                                         (48)         (47)         (48)         (47)                                   
   Spleen                                             (48)         (47)         (48)         (47)                                   
      Adenocarcinoma, Metastatic, Pancreas                                                    1 (2%)                                
   Thymus                                             (43)         (42)         (43)         (42)                                   
      Fibrous Histiocytoma                                          1 (2%)                                                          
      Histiocytic Sarcoma                                           1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (43)         (41)         (46)         (41)                                   
      Carcinoma                                                                  1 (2%)                                             
      Fibroadenoma                                     1 (2%)       2 (5%)       1 (2%)       4 (10%)                               
   Skin                                               (48)         (47)         (47)         (47)                                   
      Basal Cell Adenoma                                                                      1 (2%)                                
      Basal Cell Carcinoma                                                       1 (2%)                                             
      Fibroma                                          4 (8%)       1 (2%)       1 (2%)       5 (11%)                               
      Fibrous Histiocytoma                                          1 (2%)       1 (2%)       1 (2%)                                
      Keratoacanthoma                                  2 (4%)       3 (6%)                    2 (4%)                                
      Lipoma                                                        3 (6%)       1 (2%)                                             
      Sarcoma                                                       3 (6%)                    4 (9%)                                
      Squamous Cell Carcinoma                                                                 1 (2%)                                
      Squamous Cell Papilloma                                       1 (2%)                                                          
      Face, Squamous Cell Papilloma                                 1 (2%)                                                          
      Sebaceous Gland, Adenoma                                      1 (2%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (48)         (47)         (48)         (47)                                   
      Femur, Sarcoma, Metastatic, Skin                              1 (2%)                                                          
   Skeletal Muscle                                    (48)         (47)         (48)         (47)                                   
      Fibrous Histiocytoma, Metastatic, Skin                                                  1 (2%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (48)         (47)         (48)         (47)                                   
      Cerebrum, Astrocytoma Malignant                               1 (2%)                                                          
   Spinal Cord                                        (48)         (47)         (48)         (47)                                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   9                                                               
                                                                                                                                   
NTP Experiment-Test: 93029-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                        LEUKOMALACHITE GREEN                                   Date: 06/18/03  
Route: DOSED FEED                                                                                                 Time: 15:01:05  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS-NCTR MALE                0 PPM        91 PPM       272 PPM      543 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (48)         (47)         (48)         (47)                                   
      Alveolar/Bronchiolar Adenoma                     1 (2%)                    3 (6%)       4 (9%)                                
      Carcinoma, Metastatic, Thyroid Gland                                       1 (2%)                                             
      Fibrous Histiocytoma, Metastatic, Skin                        1 (2%)                                                          
      Histiocytic Sarcoma                                           1 (2%)                                                          
      Sarcoma, Metastatic, Skin                                     1 (2%)                    1 (2%)                                
      Squamous Cell Carcinoma, Metastatic,                                                                                          
          Preputial Gland                                                                     1 (2%)                                
      Alveolar/Bronchiolar, Adenoma                                 1 (2%)                                                          
   Nose                                               (48)         (47)         (48)         (47)                                   
   Trachea                                            (48)         (47)         (48)         (47)                                   
      Fibrous Histiocytoma                                                                    1 (2%)                                
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 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Ear                                                (1)                       (2)                                                 
      Pinna, Neural Crest Tumor                        1 (100%)                  1 (50%)                                            
   Eye                                                (48)         (47)         (48)         (47)                                   
      Lids, Sarcoma                                    1 (2%)                                                                       
   Harderian Gland                                    (48)         (47)         (48)         (47)                                   
   Zymbal's Gland                                     (46)         (43)         (47)         (47)                                   
      Carcinoma                                        1 (2%)                                 1 (2%)                                
      Squamous Cell Carcinoma                                                    1 (2%)                                             
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 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (48)         (47)         (48)         (47)                                   
      Histiocytic Sarcoma                                           1 (2%)                                                          
   Urinary Bladder                                    (48)         (47)         (48)         (47)                                   
      Transitional Epithelium, Papilloma                            1 (2%)                                                          
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
                                                                                                                                    
   Multiple Organs                                   *(48)        *(47)        *(48)        *(47)                                   
      Leukemia Mononuclear                            29 (60%)     16 (34%)     19 (40%)      7 (15%)                               
      Mesothelioma Malignant                           2 (4%)                    1 (2%)       1 (2%)                                
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  10                                                               
                                                                                                                                   
NTP Experiment-Test: 93029-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                        LEUKOMALACHITE GREEN                                   Date: 06/18/03  
Route: DOSED FEED                                                                                                 Time: 15:01:05  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS-NCTR MALE                0 PPM        91 PPM       272 PPM      543 PPM                                   
                                                                                                                                    
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 SYSTEMIC LESIONS - cont                                                                                                            
                                                                                                                                    
   Multiple Organs                                   *(48)        *(47)        *(48)        *(47)                                   
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                                                             Page  11                                                               
                                                                                                                                   
NTP Experiment-Test: 93029-03  INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (SYSTEMIC LESIONS ABRIDGED) (a)      Report: PEIRPT05
Study Type: CHRONIC                                        LEUKOMALACHITE GREEN                                   Date: 06/18/03  
Route: DOSED FEED                                                                                                 Time: 15:01:05  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         FISCHER 344 RATS-NCTR MALE                0 PPM        91 PPM       272 PPM      543 PPM                                   
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)            48          47          47          47                                       
     Total Primary Neoplasms                          140         129         122         128                                       
                                                                                                                                    
   Total Animals with Benign Neoplasms                 45          47          47          47                                       
     Total Benign Neoplasms                            98          98          92          94                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms              34          23          25          26                                       
     Total Malignant Neoplasms                         41          31          29          34                                       
                                                                                                                                    
   Total Animals with Metastatic Neoplasms                          3           1           5                                       
     Total Metastatic Neoplasm                                     10           1           6                                       
                                                                                                                                    
   Total Animals with Malignant Neoplasms                                                                                           
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                  1                       1                                                   
     Total Uncertain Neoplasms                          1                       1                                                   
                                                                                                                                    
                                                                                                                                    
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a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page  12                                                               
                                                                                                                                   
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