National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/12081

TDMS Study 99033-02 Pathology Tables

NTP Experiment-Test: 99033-02               INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a)                     Report: PEIRPT02
Study Type: SPECIAL STUDY                       TRANSGENIC MODEL EVALUATION II(ASPARTAME)                         Date: 06/05/02
Route: DOSED FEED                                                                                                 Time: 11:51:08

                                                           FINAL/MICE




       Facility:  BIORELIANCE

       Chemical CAS #:  22839-47-0

       Lock Date:  07/20/01

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All


































a  Number of animals examined microscopically at site and number of animals with lesion

                                                              Page   1


NTP Experiment-Test: 99033-02               INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a)                     Report: PEIRPT02
Study Type: SPECIAL STUDY                       TRANSGENIC MODEL EVALUATION II(ASPARTAME)                         Date: 06/05/02  
Route: DOSED FEED                                                                                                 Time: 11:51:08  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         MICE:P53+/-(C57BL/6) FEMALE               VEHICLE      3125 PPM     6250 PPM     12500        25000        50000           
                                                   CONTROL                                PPM          PPM          PPM             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially in Study                          15           15           15           15           15           15           
  Early Deaths                                                                                                                      
    Moribund Sacrifice                                 1                         1                                                  
    Natural Death                                                   1                                                               
  Survivors                                                                                                                         
    Terminal Sacrifice                                14           14           14           15           15           15           
                                                                                                                                    
  Animals Examined Microscopically                    15           15           15           15           15           15           
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Gallbladder                                        (14)                                                             (15)         
      Lymphoma Malignant                               1 (7%)                                                                       
   Intestine Large, Cecum                             (15)                                                (1)          (15)         
      Lymphoid Tissue, Lymphoma Malignant                                                                  1 (100%)                 
   Liver                                              (15)         (15)         (15)         (15)         (15)         (15)         
      Lymphoma Malignant                               1 (7%)                                                                       
      Sarcoma                                                                                 1 (7%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Pituitary Gland                                    (9)          (14)         (13)         (14)         (11)         (14)         
      Pars Distalis, Adenoma                           1 (11%)                                                                      
   Thyroid Gland                                      (14)         (13)         (14)         (14)         (13)         (15)         
      Follicular Cell, Adenoma                                                                             1 (8%)                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   2                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02               INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a)                     Report: PEIRPT02
Study Type: SPECIAL STUDY                       TRANSGENIC MODEL EVALUATION II(ASPARTAME)                         Date: 06/05/02  
Route: DOSED FEED                                                                                                 Time: 11:51:08  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         MICE:P53+/-(C57BL/6) FEMALE               VEHICLE      3125 PPM     6250 PPM     12500        25000        50000           
                                                   CONTROL                                PPM          PPM          PPM             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Lymph Node                                                                                             (1)                       
      Pancreatic, Lymphoma Malignant                                                                       1 (100%)                 
      Renal, Lymphoma Malignant                                                                            1 (100%)                 
   Lymph Node, Mandibular                             (15)         (13)         (13)         (15)         (15)         (14)         
      Lymphoma Malignant                                                                                   1 (7%)                   
   Lymph Node, Mesenteric                             (15)         (14)         (14)         (14)         (15)         (15)         
      Sarcoma                                                                                 1 (7%)                                
   Lymph Node, Mediastinal                            (12)         (13)         (13)         (12)         (13)         (15)         
      Lymphoma Malignant                                                                                   1 (8%)                   
   Spleen                                             (15)         (15)         (15)         (15)         (15)         (15)         
      Lymphoma Malignant                               1 (7%)                                              1 (7%)                   
   Thymus                                             (14)         (14)         (14)         (15)         (15)         (15)         
      Lymphoma Malignant                               1 (7%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Mammary Gland                                      (13)         (14)         (14)         (15)         (12)         (15)         
      Carcinoma                                        1 (8%)                                 1 (7%)                                
   Skin                                               (15)                      (1)                                    (15)         
      Subcutaneous Tissue, Fibrosarcoma                                          1 (100%)                                           
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (15)                      (1)                                    (15)         
      Femur, Osteosarcoma                              1 (7%)                                                           1 (7%)      
      Vertebra, Osteosarcoma                                                     1 (100%)                                           
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   3                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02               INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a)                     Report: PEIRPT02
Study Type: SPECIAL STUDY                       TRANSGENIC MODEL EVALUATION II(ASPARTAME)                         Date: 06/05/02  
Route: DOSED FEED                                                                                                 Time: 11:51:08  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         MICE:P53+/-(C57BL/6) FEMALE               VEHICLE      3125 PPM     6250 PPM     12500        25000        50000           
                                                   CONTROL                                PPM          PPM          PPM             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (15)         (15)         (14)         (15)         (15)         (15)         
      Lymphoma Malignant                               1 (7%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (15)         (14)         (15)         (15)         (15)         (15)         
      Lymphoma Malignant                               1 (7%)                                                                       
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
                                                                                                                                    
   Multiple Organs                                   *(15)        *(15)        *(15)        *(15)        *(15)        *(15)         
      Lymphoma Malignant                               1 (7%)                                              1 (7%)                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   4                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02               INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a)                     Report: PEIRPT02
Study Type: SPECIAL STUDY                       TRANSGENIC MODEL EVALUATION II(ASPARTAME)                         Date: 06/05/02  
Route: DOSED FEED                                                                                                 Time: 11:51:08  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         MICE:P53+/-(C57BL/6) FEMALE               VEHICLE      3125 PPM     6250 PPM     12500        25000        50000           
                                                   CONTROL                                PPM          PPM          PPM             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)             4                       2           2           1           1               
     Total Primary Neoplasms                            4                       2           3           2           1               
                                                                                                                                    
   Total Animals with Benign Neoplasms                  1                                               1                           
     Total Benign Neoplasms                             1                                               1                           
                                                                                                                                    
   Total Animals with Malignant Neoplasms               3                       2           2           1           1               
     Total Malignant Neoplasms                          3                       2           3           1           1               
                                                                                                                                    
   Total Animals with Metastatic Neoplasms                                                                                          
     Total Metastatic Neoplasm                                                                                                      
                                                                                                                                    
   Total Animals with Malignant Neoplasms                                                                                           
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                                                                                              
     Total Uncertain Neoplasms                                                                                                      
                                                                                                                                    
                                                                                                                                    
____________________________________________________________________________________________________________________________________
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   5                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02               INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a)                     Report: PEIRPT02
Study Type: SPECIAL STUDY                       TRANSGENIC MODEL EVALUATION II(ASPARTAME)                         Date: 06/05/02  
Route: DOSED FEED                                                                                                 Time: 11:51:08  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         MICE:P53+/-(C57BL/6) MALE                 VEHICLE      3125 PPM     6250 PPM     12500        25000        50000           
                                                   CONTROL                                PPM          PPM          PPM             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially in Study                          15           15           15           15           15           15           
  Early Deaths                                                                                                                      
    Natural Death                                      1                         1                         1                        
    Moribund Sacrifice                                                           1                                      1           
  Survivors                                                                                                                         
    Terminal Sacrifice                                14           15           13           15           14           14           
                                                                                                                                    
  Animals Examined Microscopically                    15           15           15           15           15           15           
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Intestine Small, Duodenum                          (14)                                                             (15)         
      Polyp Adenomatous                                                                                                 1 (7%)      
   Liver                                              (14)         (15)         (15)         (15)         (14)         (15)         
      Lymphoma Malignant                                                         2 (13%)                                            
   Salivary Glands                                    (14)         (1)          (1)                       (1)          (15)         
      Lymphoma Malignant                                                         1 (100%)                                           
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Prostate                                           (14)         (15)         (15)         (15)         (14)         (15)         
      Sarcoma                                                                                              1 (7%)                   
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   6                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02               INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a)                     Report: PEIRPT02
Study Type: SPECIAL STUDY                       TRANSGENIC MODEL EVALUATION II(ASPARTAME)                         Date: 06/05/02  
Route: DOSED FEED                                                                                                 Time: 11:51:08  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         MICE:P53+/-(C57BL/6) MALE                 VEHICLE      3125 PPM     6250 PPM     12500        25000        50000           
                                                   CONTROL                                PPM          PPM          PPM             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Lymph Node, Mandibular                             (14)         (15)         (14)         (15)         (14)         (15)         
      Lymphoma Malignant                                                         1 (7%)                                             
   Lymph Node, Mesenteric                             (14)         (14)         (15)         (13)         (14)         (15)         
      Lymphoma Malignant                                                         1 (7%)                                             
   Lymph Node, Mediastinal                            (13)         (14)         (14)         (13)         (12)         (13)         
      Lymphoma Malignant                                                         2 (14%)                                            
   Spleen                                             (14)         (15)         (15)         (15)         (14)         (15)         
      Lymphoma Malignant                                                         2 (13%)                                            
   Thymus                                             (14)         (15)         (15)         (14)         (15)         (14)         
      Lymphoma Malignant                                                         2 (13%)                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Skin                                               (15)                                                             (15)         
      Subcutaneous Tissue, Sarcoma                                                                                      1 (7%)      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (15)         (15)         (15)         (15)         (14)         (15)         
      Lymphoma Malignant                                                         2 (13%)                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   7                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02               INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a)                     Report: PEIRPT02
Study Type: SPECIAL STUDY                       TRANSGENIC MODEL EVALUATION II(ASPARTAME)                         Date: 06/05/02  
Route: DOSED FEED                                                                                                 Time: 11:51:08  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         MICE:P53+/-(C57BL/6) MALE                 VEHICLE      3125 PPM     6250 PPM     12500        25000        50000           
                                                   CONTROL                                PPM          PPM          PPM             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (14)         (15)         (15)         (15)         (14)         (15)         
      Lymphoma Malignant                                                         1 (7%)                                             
                                                                                                                                    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SYSTEMIC LESIONS                                                                                                                   
                                                                                                                                    
   Multiple Organs                                   *(15)        *(15)        *(15)        *(15)        *(15)        *(15)         
      Lymphoma Malignant                                                         2 (13%)                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
     *   Number of animals with any tissue examined microscopically                                                                 
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   8                                                               
                                                                                                                                   
NTP Experiment-Test: 99033-02               INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a)                     Report: PEIRPT02
Study Type: SPECIAL STUDY                       TRANSGENIC MODEL EVALUATION II(ASPARTAME)                         Date: 06/05/02  
Route: DOSED FEED                                                                                                 Time: 11:51:08  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         MICE:P53+/-(C57BL/6) MALE                 VEHICLE      3125 PPM     6250 PPM     12500        25000        50000           
                                                   CONTROL                                PPM          PPM          PPM             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
TUMOR SUMMARY                                                                                                                       
                                                                                                                                    
   Total Animals with Primary Neoplasms (b)                                     2                       1           2               
     Total Primary Neoplasms                                                    2                       1           2               
                                                                                                                                    
   Total Animals with Benign Neoplasms                                                                              1               
     Total Benign Neoplasms                                                                                         1               
                                                                                                                                    
   Total Animals with Malignant Neoplasms                                       2                       1           1               
     Total Malignant Neoplasms                                                  2                       1           1               
                                                                                                                                    
   Total Animals with Metastatic Neoplasms                                                                                          
     Total Metastatic Neoplasm                                                                                                      
                                                                                                                                    
   Total Animals with Malignant Neoplasms                                                                                           
   Uncertain Primary Site                                                                                                           
                                                                                                                                    
   Total Animals with Neoplasms Uncertain-                                                                                          
   Benign or Malignant                                                                                                              
     Total Uncertain Neoplasms                                                                                                      
                                                                                                                                    
                                                                                                                                    
____________________________________________________________________________________________________________________________________
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Primary tumors: all tumors except metastatic tumors                                                                              
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page   9                                                               
                                                                                                                                   
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