https://ntp.niehs.nih.gov/go/13050

TDMS Study 99033-03 Pathology Tables

NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03
Route: DOSED FEED                                                                                                 Time: 11:45:14
                                                      FINAL #3 TG:P16 MICE
       Facility:  BIORELIANCE
       Chemical CAS #:  22839-47-0
       Lock Date:  07/20/01
       Cage Range:  All
       Reasons For Removal:    All
       Removal Date Range:     All
       Treatment Groups:       Include All
Note:  Animals arranged according to days on test
                                                              Page   1
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 1| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 3| 9| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    VEHICLE                                | 8| 9| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9|                             |     L      |
    CONTROL                                | 8| 2| 1| 3| 5| 6| 7| 2| 4| 9| 0| 1| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | A  +  +  M  +  +  +  +  +  +  +  M  +  +  +                              |  12        |
                                           |__________________________________________________________________________|____________|
   Gallbladder                             | A  A  +  +  +  +  +  M  +  +  +  +  +  +  +                              |  12        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | A  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | A  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | A  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | A  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | A  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |    X     X                                                               |          2 |
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | A  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Tongue                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Blood Vessel                            | M  +  +  +  +  +  +  +  M  +  M  +  +  +  +                              |  12        |
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page   2                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 1| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 3| 9| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    VEHICLE                                | 8| 9| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9|                             |     L      |
    CONTROL                                | 8| 2| 1| 3| 5| 6| 7| 2| 4| 9| 0| 1| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 CARDIOVASCULAR SYSTEM - cont              |                                                                          |            |
                                           |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | M  M  M  M  M  +  +  +  +  +  M  M  +  M  +                              |   7        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | M  +  I  +  I  +  I  +  +  M  +  +  +  +  +                              |  10        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Clitoral Gland                          | M  M  M  M  +  +  M  M  M  +  +  +  +  +  +                              |   8        |
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |          X     X  X                                                      |          3 |
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Vagina                                  | +  +  +  M  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page   3                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 1| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 3| 9| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    VEHICLE                                | 8| 9| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9|                             |     L      |
    CONTROL                                | 8| 2| 1| 3| 5| 6| 7| 2| 4| 9| 0| 1| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
                                           |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |          X     X  X           X                                          |          4 |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
      Histiocytic Sarcoma                  |    X                                                                     |          1 |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
      Histiocytic Sarcoma                  |    X                                                                     |          1 |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
      Histiocytic Sarcoma                  |    X                                                                     |          1 |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |    X                                                                     |          1 |
                                           |__________________________________________________________________________|____________|
   Thymus                                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
      Histiocytic Sarcoma                  |    X                                                                     |          1 |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | A  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Skin                                    | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page   4                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 1| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 3| 9| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    VEHICLE                                | 8| 9| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9|                             |     L      |
    CONTROL                                | 8| 2| 1| 3| 5| 6| 7| 2| 4| 9| 0| 1| 3| 4| 5|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 NERVOUS SYSTEM - cont                     |                                                                          |            |
                                           |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Larynx                                  | M  +  +  +  M  +  I  M  M  M  I  M  M  M  I                              |   4        |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Alveolar/Bronchiolar Adenoma         |                         X                                                |          1 |
      Histiocytic Sarcoma                  |    X                                                                     |          1 |
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Zymbal's Gland                          | +  +  +  M  M  M  +  M  +  +  +  +  +  +  M                              |  10        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 __________________________________________________________________________________________________________________________________ 
 SYSTEMIC LESIONS                          |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Multiple Organs                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |    X     X     X  X           X                                          |          5 |
 __________________________________________________________________________________________________________________________________ 
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page   5                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 4| 4| 5| 5| 5|                             |     A      |
    3125 PPM                               | 9| 9| 0| 0| 0| 0| 0| 0| 0| 1| 9| 9| 0| 0| 0|                             |     L      |
                                           | 7| 8| 0| 2| 3| 4| 6| 7| 9| 0| 6| 9| 1| 5| 8|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Gallbladder                             |                         +                                                |   1        |
      Histiocytic Sarcoma                  |                         X                                                |          1 |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |                         X                                                |          1 |
                                           |__________________________________________________________________________|____________|
   Pancreas                                |                         +                                                |   1        |
      Histiocytic Sarcoma                  |                         X                                                |          1 |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  +  +  +  I  +  +  I  +  +  +                              |  13        |
      Adenoma                              |       X                                                                  |          1 |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page   6                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 4| 4| 5| 5| 5|                             |     A      |
    3125 PPM                               | 9| 9| 0| 0| 0| 0| 0| 0| 0| 1| 9| 9| 0| 0| 0|                             |     L      |
                                           | 7| 8| 0| 2| 3| 4| 6| 7| 9| 0| 6| 9| 1| 5| 8|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM - cont                     |                                                                          |            |
                                           |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |                         X                 X                              |          2 |
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |                         X                                                |          1 |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  A  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page   7                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 5| 5| 5| 5| 5| 5| 5| 5| 4| 4| 5| 5| 5|                             |     A      |
    3125 PPM                               | 9| 9| 0| 0| 0| 0| 0| 0| 0| 1| 9| 9| 0| 0| 0|                             |     L      |
                                           | 7| 8| 0| 2| 3| 4| 6| 7| 9| 0| 6| 9| 1| 5| 8|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 RESPIRATORY SYSTEM - cont                 |                                                                          |            |
                                           |                                                                          |            |
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +     +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +                                +                                       |   2        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
 SYSTEMIC LESIONS                          |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Multiple Organs                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |                         X                 X                              |          2 |
 __________________________________________________________________________________________________________________________________ 
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page   8                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 0| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 8| 6| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|                             |     A      |
    6250 PPM                               | 1| 1| 1| 2| 2| 2| 1| 1| 1| 1| 1| 1| 2| 2| 2|                             |     L      |
                                           | 7| 6| 1| 0| 3| 5| 2| 3| 4| 5| 8| 9| 1| 2| 4|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | A                                   +                                    |   1        |
      Leiomyoma                            |                                     X                                    |          1 |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | A                                                                        |            |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | A                                                                        |            |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |          X                                                               |          1 |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | A  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | A  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  13        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hemangiosarcoma                      |                               X                                          |          1 |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | A  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | A  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | I  I  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Tissue NOS                              |    +                                                                     |   1        |
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page   9                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 0| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 8| 6| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|                             |     A      |
    6250 PPM                               | 1| 1| 1| 2| 2| 2| 1| 1| 1| 1| 1| 1| 2| 2| 2|                             |     L      |
                                           | 7| 6| 1| 0| 3| 5| 2| 3| 4| 5| 8| 9| 1| 2| 4|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |          X     X                                                         |          2 |
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | A  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | A  A  +  +  +  +  +  +  +  +  +  +  I  +  +                              |  12        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
      Hemangiosarcoma                      | X                                                                        |          1 |
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  10                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 1| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 0| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 8| 6| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|                             |     A      |
    6250 PPM                               | 1| 1| 1| 2| 2| 2| 1| 1| 1| 1| 1| 1| 2| 2| 2|                             |     L      |
                                           | 7| 6| 1| 0| 3| 5| 2| 3| 4| 5| 8| 9| 1| 2| 4|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 RESPIRATORY SYSTEM - cont                 |                                                                          |            |
                                           |                                                                          |            |
      Histiocytic Sarcoma                  |          X                                                               |          1 |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | A  A  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         |    +                                                                     |   1        |
 __________________________________________________________________________________________________________________________________ 
 SYSTEMIC LESIONS                          |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Multiple Organs                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |          X     X                                                         |          2 |
 __________________________________________________________________________________________________________________________________ 
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  11                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|                             |     A      |
    12500                                  | 2| 2| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|                             |     L      |
    PPM                                    | 8| 9| 3| 6| 7| 0| 1| 2| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  M  +  M  +  +  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | I  +  +  +  +  +  +  +  +  +  M  +  +  +  I                              |  12        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |                   X           X                                          |          2 |
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  12                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|                             |     A      |
    12500                                  | 2| 2| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|                             |     L      |
    PPM                                    | 8| 9| 3| 6| 7| 0| 1| 2| 4| 5| 6| 7| 8| 9| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
                                           |                                                                          |            |
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  +  +  +  +  I  +  +  +  +  +  +  +  M  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  M  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
 SYSTEMIC LESIONS                          |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Multiple Organs                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  13                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 _____________________________________________________________________________________________________________________      T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|                             |     A      |
    12500                                  | 2| 2| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4|                             |     L      |
    PPM                                    | 8| 9| 3| 6| 7| 0| 1| 2| 4| 5| 6| 7| 8| 9| 0|                             |            |
 __________________________________________________________________________________________________________________________________ 
 SYSTEMIC LESIONS - cont                   |                                                                          |            |
                                           |                                                                          |            |
      Histiocytic Sarcoma                  |                   X           X                                          |          2 |
 __________________________________________________________________________________________________________________________________ 
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  14                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|                             |     A      |
    25000                                  | 4| 4| 4| 5| 5| 5| 4| 4| 4| 4| 4| 4| 5| 5| 5|                             |     L      |
    PPM                                    | 2| 3| 5| 1| 2| 5| 1| 4| 6| 7| 8| 9| 0| 3| 4|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  I  +  +  +  +  +  I  I  +  +  M  +  +  +                              |  11        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |                         X     X                                          |          2 |
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  15                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|                             |     A      |
    25000                                  | 4| 4| 4| 5| 5| 5| 4| 4| 4| 4| 4| 4| 5| 5| 5|                             |     L      |
    PPM                                    | 2| 3| 5| 1| 2| 5| 1| 4| 6| 7| 8| 9| 0| 3| 4|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
                                           |                                                                          |            |
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | M  I  +  +  +  +  +  +  +  +  +  +  +  +  M                              |  12        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
 SYSTEMIC LESIONS                          |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Multiple Organs                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  16                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 _____________________________________________________________________________________________________________________      T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|                             |     A      |
    25000                                  | 4| 4| 4| 5| 5| 5| 4| 4| 4| 4| 4| 4| 5| 5| 5|                             |     L      |
    PPM                                    | 2| 3| 5| 1| 2| 5| 1| 4| 6| 7| 8| 9| 0| 3| 4|                             |            |
 __________________________________________________________________________________________________________________________________ 
 SYSTEMIC LESIONS - cont                   |                                                                          |            |
                                           |                                                                          |            |
      Histiocytic Sarcoma                  |                         X     X                                          |          2 |
      Leukemia Erythrocytic                |             X                                                            |          1 |
 __________________________________________________________________________________________________________________________________ 
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  17                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 7| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|                             |     A      |
    50000                                  | 6| 5| 5| 6| 6| 6| 6| 5| 5| 6| 6| 6| 6| 6| 7|                             |     L      |
    PPM                                    | 0| 8| 9| 1| 4| 8| 9| 6| 7| 2| 3| 5| 6| 7| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Gallbladder                             | +  +  +  M  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  | X                                   X                                    |          2 |
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |                                     X                                    |          1 |
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Tongue                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  18                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 7| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|                             |     A      |
    50000                                  | 6| 5| 5| 6| 6| 6| 6| 5| 5| 6| 6| 6| 6| 6| 7|                             |     L      |
    PPM                                    | 0| 8| 9| 1| 4| 8| 9| 6| 7| 2| 3| 5| 6| 7| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 CARDIOVASCULAR SYSTEM - cont              |                                                                          |            |
                                           |                                                                          |            |
   Blood Vessel                            | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |                                     X                                    |          1 |
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  +  +  M  M  M  +  M  M  M  M  M  M  M  M                              |   4        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  +  +  M  +  +  +  I  M  +  +  +  +                              |  12        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Clitoral Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Ovary                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  | X                                   X                                    |          2 |
                                           |__________________________________________________________________________|____________|
   Uterus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Vagina                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  19                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 7| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|                             |     A      |
    50000                                  | 6| 5| 5| 6| 6| 6| 6| 5| 5| 6| 6| 6| 6| 6| 7|                             |     L      |
    PPM                                    | 0| 8| 9| 1| 4| 8| 9| 6| 7| 2| 3| 5| 6| 7| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  | X     X                             X                                    |          3 |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  M  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  +  +  M  +  +  +  +  +  +  +  M  +  M  +                              |  12        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  | X                                   X                                    |          2 |
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hemangiosarcoma                      |          X                                                               |          1 |
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Meninges, Histiocytic Sarcoma        | X                                                                        |          1 |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  20                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 7| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|                             |     A      |
    50000                                  | 6| 5| 5| 6| 6| 6| 6| 5| 5| 6| 6| 6| 6| 6| 7|                             |     L      |
    PPM                                    | 0| 8| 9| 1| 4| 8| 9| 6| 7| 2| 3| 5| 6| 7| 0|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 NERVOUS SYSTEM - cont                     |                                                                          |            |
                                           |                                                                          |            |
   Peripheral Nerve                        | +                                                                        |   1        |
                                           |__________________________________________________________________________|____________|
   Spinal Cord                             | +                                                                        |   1        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Larynx                                  | I  +  I  I  +  I  +  I  M  I  I  M  +  I  +                              |   5        |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Alveolar/Bronchiolar Carcinoma       |                                        X                                 |          1 |
      Histiocytic Sarcoma                  | X                                   X                                    |          2 |
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Adenoma                              |             X                                                            |          1 |
                                           |__________________________________________________________________________|____________|
   Zymbal's Gland                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
 SYSTEMIC LESIONS                          |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Multiple Organs                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  21                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 7| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6|                             |            |
 _____________________________________________________________________________________________________________________      T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) FEMALE       | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5| 5|                             |     A      |
    50000                                  | 6| 5| 5| 6| 6| 6| 6| 5| 5| 6| 6| 6| 6| 6| 7|                             |     L      |
    PPM                                    | 0| 8| 9| 1| 4| 8| 9| 6| 7| 2| 3| 5| 6| 7| 0|                             |            |
 __________________________________________________________________________________________________________________________________ 
 SYSTEMIC LESIONS - cont                   |                                                                          |            |
                                           |                                                                          |            |
      Histiocytic Sarcoma                  | X     X                             X                                    |          3 |
 __________________________________________________________________________________________________________________________________ 
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  22                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 3| 4|                             |     A      |
    VEHICLE                                | 0| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 9| 0|                             |     L      |
    CONTROL                                | 1| 1| 2| 3| 4| 5| 7| 8| 9| 0| 2| 4| 5| 6| 3|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Gallbladder                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  I                              |  14        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  +  M  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | A  +  +  +  +  +  +  A  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Tongue                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Blood Vessel                            | +  +  +  +  +  +  +  +  +  I  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  23                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 3| 4|                             |     A      |
    VEHICLE                                | 0| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 9| 0|                             |     L      |
    CONTROL                                | 1| 1| 2| 3| 4| 5| 7| 8| 9| 0| 2| 4| 5| 6| 3|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 CARDIOVASCULAR SYSTEM - cont              |                                                                          |            |
                                           |                                                                          |            |
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  M  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | M  M  +  +  M  M  +  +  +  +  +  M  +  M  M                              |   8        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  I  +  +  +  +  +  +  +  +  +  +  I                              |  13        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  M  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Preputial Gland                         | +  +  +  +  +  +  +  M  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  24                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 3| 4|                             |     A      |
    VEHICLE                                | 0| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 9| 0|                             |     L      |
    CONTROL                                | 1| 1| 2| 3| 4| 5| 7| 8| 9| 0| 2| 4| 5| 6| 3|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
                                           |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |                X              X                                          |          2 |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | M  +  +  +  +  +  +  M  +  +  M  +  +  +  +                              |  12        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M                              |            |
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  25                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 3| 4|                             |     A      |
    VEHICLE                                | 0| 9| 9| 9| 9| 9| 9| 9| 9| 0| 0| 0| 0| 9| 0|                             |     L      |
    CONTROL                                | 1| 1| 2| 3| 4| 5| 7| 8| 9| 0| 2| 4| 5| 6| 3|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 RESPIRATORY SYSTEM - cont                 |                                                                          |            |
                                           |                                                                          |            |
   Larynx                                  | I  +  M  M  M  M  M  M  M  M  M  +  M  M  M                              |   2        |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  M  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Zymbal's Gland                          | M  +  +  +  +  +  M  +  +  +  +  +  +  +  +                              |  13        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
 SYSTEMIC LESIONS                          |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Multiple Organs                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  |                X              X                                          |          2 |
 __________________________________________________________________________________________________________________________________ 
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  26                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 3| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 2| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    3125 PPM                               | 0| 0| 0| 1| 1| 1| 1| 1| 2| 0| 1| 1| 1| 1| 1|                             |     L      |
                                           | 6| 8| 9| 4| 5| 6| 7| 8| 0| 7| 0| 1| 2| 3| 9|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  | X                                                                        |          1 |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  | X                                                                        |          1 |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  I  +  +  I  +  +  +  I  +  +  +  I  I  +                              |  10        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  27                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 3| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 2| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    3125 PPM                               | 0| 0| 0| 1| 1| 1| 1| 1| 2| 0| 1| 1| 1| 1| 1|                             |     L      |
                                           | 6| 8| 9| 4| 5| 6| 7| 8| 0| 7| 0| 1| 2| 3| 9|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  | X                                                                        |          1 |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  | X                                                                        |          1 |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | M  +  +  +  I  +  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Hemangiosarcoma                      |          X                                                               |          1 |
      Histiocytic Sarcoma                  | X                                                                        |          1 |
                                           |__________________________________________________________________________|____________|
   Thymus                                  | M  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M                              |            |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         |                            +                                             |   1        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  | X                                                                        |          1 |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  28                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 3| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 2| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    3125 PPM                               | 0| 0| 0| 1| 1| 1| 1| 1| 2| 0| 1| 1| 1| 1| 1|                             |     L      |
                                           | 6| 8| 9| 4| 5| 6| 7| 8| 0| 7| 0| 1| 2| 3| 9|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  | X                                                                        |          1 |
 __________________________________________________________________________________________________________________________________ 
 SYSTEMIC LESIONS                          |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Multiple Organs                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Histiocytic Sarcoma                  | X                                                                        |          1 |
 __________________________________________________________________________________________________________________________________ 
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  29                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    6250 PPM                               | 2| 2| 2| 2| 2| 3| 3| 3| 3| 2| 2| 2| 2| 3| 3|                             |     L      |
                                           | 2| 4| 6| 7| 9| 1| 2| 3| 5| 1| 3| 5| 8| 0| 4|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | I  I  I  I  +  +  +  I  +  +  +  +  +  +  +                              |  10        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  30                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    6250 PPM                               | 2| 2| 2| 2| 2| 3| 3| 3| 3| 2| 2| 2| 2| 3| 3|                             |     L      |
                                           | 2| 4| 6| 7| 9| 1| 2| 3| 5| 1| 3| 5| 8| 0| 4|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
                                           |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  M  +  +  M  +  +  +  +  +  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M                              |            |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
 SYSTEMIC LESIONS                          |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Multiple Organs                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  31                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    12500                                  | 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 5| 3| 4| 4| 4|                             |     L      |
    PPM                                    | 6| 8| 9| 0| 2| 3| 5| 6| 8| 9| 0| 7| 1| 4| 7|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Liver                                   | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | A  +  +  +  +  +  +  +  +  +  +  +  +  I  I                              |  12        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Testes                                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  32                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    12500                                  | 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 5| 3| 4| 4| 4|                             |     L      |
    PPM                                    | 6| 8| 9| 0| 2| 3| 5| 6| 8| 9| 0| 7| 1| 4| 7|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
                                           |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | A  +  +  +  +  +  +  +  +  M  +  +  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Thymus                                  | A  +  +  +  +  +  +  +  +  +  +  M  +  +  +                              |  13        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M                              |            |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Alveolar/Bronchiolar Adenoma         |                                  X                                       |          1 |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  33                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    12500                                  | 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 5| 3| 4| 4| 4|                             |     L      |
    PPM                                    | 6| 8| 9| 0| 2| 3| 5| 6| 8| 9| 0| 7| 1| 4| 7|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 URINARY SYSTEM - cont                     |                                                                          |            |
                                           |                                                                          |            |
   Kidney                                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 __________________________________________________________________________________________________________________________________ 
 SYSTEMIC LESIONS                          |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Multiple Organs                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  34                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 2| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    25000                                  | 6| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 5| 6|                             |     L      |
    PPM                                    | 5| 1| 2| 3| 4| 5| 7| 8| 9| 0| 1| 3| 4| 6| 2|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | I  +  I  I  +  I  +  I  +  +  +  +  +  +  +                              |  10        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  M  +  +  +  +  +  +  +  +  M  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Testes                                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  35                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 0| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 2| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    25000                                  | 6| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 5| 6|                             |     L      |
    PPM                                    | 5| 1| 2| 3| 4| 5| 7| 8| 9| 0| 1| 3| 4| 6| 2|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
                                           |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | M  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | A  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  +  +  +  +  I  +  +  +  +  +  M  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M                              |            |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
 SYSTEMIC LESIONS                          |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Multiple Organs                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  36                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    50000                                  | 6| 6| 7| 7| 7| 7| 7| 7| 8| 6| 6| 7| 7| 7| 7|                             |     L      |
    PPM                                    | 7| 9| 0| 1| 2| 5| 8| 9| 0| 6| 8| 3| 4| 6| 7|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 ALIMENTARY SYSTEM                         |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Esophagus                               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Gallbladder                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Colon                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Rectum                 | +  +  +  M  +  +  +  +  +  +  +  M  +  +  +                              |  13        |
                                           |__________________________________________________________________________|____________|
   Intestine Large, Cecum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Duodenum               | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Jejunum                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Intestine Small, Ileum                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Liver                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Pancreas                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Salivary Glands                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Forestomach                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Stomach, Glandular                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Tongue                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 CARDIOVASCULAR SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Blood Vessel                            | +  M  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  37                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    50000                                  | 6| 6| 7| 7| 7| 7| 7| 7| 8| 6| 6| 7| 7| 7| 7|                             |     L      |
    PPM                                    | 7| 9| 0| 1| 2| 5| 8| 9| 0| 6| 8| 3| 4| 6| 7|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 CARDIOVASCULAR SYSTEM - cont              |                                                                          |            |
                                           |                                                                          |            |
   Heart                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 ENDOCRINE SYSTEM                          |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Adrenal Cortex                          | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Adrenal Medulla                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Islets, Pancreatic                      | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Parathyroid Gland                       | +  M  M  +  M  +  M  +  +  M  +  M  +  M  +                              |   8        |
                                           |__________________________________________________________________________|____________|
   Pituitary Gland                         | +  +  +  I  +  +  +  I  I  +  +  I  +  +  +                              |  11        |
                                           |__________________________________________________________________________|____________|
   Thyroid Gland                           | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 GENERAL BODY SYSTEM                       |                                                                          |            |
    None                                   |                                                                          |            |
 _____________________________________________________________________________________________________________________|            |
 GENITAL SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Epididymis                              | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Preputial Gland                         | +  +  +  +  +  +  +  +  +  M  +  +  +  +  +                              |  14        |
                                           |__________________________________________________________________________|____________|
   Prostate                                | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Seminal Vesicle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Testes                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 HEMATOPOIETIC SYSTEM                      |                                                                          |            |
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  38                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    50000                                  | 6| 6| 7| 7| 7| 7| 7| 7| 8| 6| 6| 7| 7| 7| 7|                             |     L      |
    PPM                                    | 7| 9| 0| 1| 2| 5| 8| 9| 0| 6| 8| 3| 4| 6| 7|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 HEMATOPOIETIC SYSTEM - cont               |                                                                          |            |
                                           |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone Marrow                             | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mandibular                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mesenteric                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Lymph Node, Mediastinal                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Spleen                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Thymus                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 INTEGUMENTARY SYSTEM                      |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Mammary Gland                           | M  M  M  M  M  M  M  M  M  M  M  M  M  M  M                              |            |
                                           |__________________________________________________________________________|____________|
   Skin                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 MUSCULOSKELETAL SYSTEM                    |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Bone                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Skeletal Muscle                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 NERVOUS SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Brain                                   | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 RESPIRATORY SYSTEM                        |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Larynx                                  | +  M  M  +  M  M  I  M  +  I  I  M  M  M  I                              |   3        |
 _____________________________________________________________________________________________________________________|____________|
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  39                                                               
NTP Experiment-Test: 99033-03          NEOPLASMS BY INDIVIDUAL ANIMAL (SYSTEMIC LESIONS ABRIDGED)                 Report: PEIRPT17  
Study Type: SUBCHRON 26-WEEK                    TRANSGENIC MODEL EVALUATION II (ASPARTAME)                        Date: 03/05/03    
Route: DOSED FEED                                                                                                 Time: 11:45:14    
 __________________________________________________________________________________________________________________________________ 
                                           | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2|                             |            |
                             DAY ON TEST   | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7|                             |            |
                                           | 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| 5| 5| 5| 5| 5|                             |            |
 __________________________________________|__________________________________________________________________________|     T (*)  |
                                           | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     O      |
   P16(INK4A)/(+/-) (C57BL/6) MALE         | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0|                             |     T      |
                               ANIMAL ID   | 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4|                             |     A      |
    50000                                  | 6| 6| 7| 7| 7| 7| 7| 7| 8| 6| 6| 7| 7| 7| 7|                             |     L      |
    PPM                                    | 7| 9| 0| 1| 2| 5| 8| 9| 0| 6| 8| 3| 4| 6| 7|                             |            |
 _____________________________________________________________________________________________________________________|____________|
 RESPIRATORY SYSTEM - cont                 |                                                                          |            |
                                           |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Lung                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
      Alveolar/Bronchiolar Adenoma         |    X                                                                     |          1 |
                                           |__________________________________________________________________________|____________|
   Nose                                    | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Trachea                                 | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 _____________________________________________________________________________________________________________________|            |
 SPECIAL SENSES SYSTEM                     |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Eye                                     | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Harderian Gland                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Zymbal's Gland                          | +  +  +  +  M  +  +  +  +  +  +  +  +  +  +                              |  14        |
 _____________________________________________________________________________________________________________________|            |
 URINARY SYSTEM                            |                                                                          |            |
                                           |__________________________________________________________________________|____________|
   Kidney                                  | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
                                           |__________________________________________________________________________|____________|
   Urinary Bladder                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
 SYSTEMIC LESIONS                          |                                                                          |            |
                                            __________________________________________________________________________|____________|
   Multiple Organs                         | +  +  +  +  +  +  +  +  +  +  +  +  +  +  +                              |  15        |
 __________________________________________________________________________________________________________________________________ 
                         * : Total animals with tissue examined microscopically; total animals with tumor                           
                         + : Tissue examined microscopically                      M : Missing tissue                                
                         X : Lesion present                                       A : Autolysis precludes evaluation                
                         I : Insufficient tissue                              BLANK : Not examined microscopically                  
                                                             Page  40                                                               
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