Skip to Main Navigation
Skip to Page Content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
https://ntp.niehs.nih.gov/go/13052

TDMS Study 99033-03 Pathology Tables

NTP Experiment-Test: 99033-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT18
Study Type: SUBCHRON 26-WEEK                         WITH AVERAGE SEVERITY GRADES[b]                              Date: 03/05/03
Route: DOSED FEED                                 TRANSGENIC MODEL EVALUATION II (ASPARTAME)                      Time: 11:45:56

                                                      FINAL #3 TG:P16 MICE




       Facility:  BIORELIANCE

       Chemical CAS #:  22839-47-0

       Lock Date:  07/20/01

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include All

































a  Number of animals examined microscopically at site and number of animals with lesion
b  Average severity grade (1-minimal;2-mild;3-moderate;4-marked)

                                                              Page   1


NTP Experiment-Test: 99033-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT18  
Study Type: SUBCHRON 26-WEEK                         WITH AVERAGE SEVERITY GRADES[b]                              Date: 03/05/03    
Route: DOSED FEED                                 TRANSGENIC MODEL EVALUATION II (ASPARTAME)                      Time: 11:45:56    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         P16(INK4A)/(+/-) (C57BL/6) FEMALE            VEHICLE      3125 PPM     6250 PPM     12500        25000        50000        
                                                      CONTROL                                PPM          PPM          PPM          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially In Study                          15           15           15           15           15           15           
  Early Deaths                                                                                                                      
    Natural Death                                      2                         2                                                  
    Moribund Sacrifice                                                                                                  1           
  Survivors                                                                                                                         
    Terminal Sacrifice                                13           15           13           15           15           14           
                                                                                                                                    
  Animals Examined Microscopically                    15           15           15           15           15           15           
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Liver                                              (15)         (15)         (15)         (15)         (15)         (15)         
      Hematopoietic Cell Proliferation                                                                                  2 [2.0]     
      Infiltration Cellular, Focal, Lymphocyte                                                                          1 [1.0]     
      Infiltration Cellular, Lymphocyte                1 [2.0]                                                                      
      Inflammation, Chronic Active                     1 [1.0]                                                                      
      Necrosis, Focal                                                                                      1 [1.0]      2 [1.0]     
      Hepatocyte, Necrosis, Focal                                   1 [1.0]                                                         
      Hepatocyte, Periportal, Vacuolization                                                                                         
        Cytoplasmic                                                                                                     2 [1.0]     
   Salivary Glands                                    (15)                                                             (15)         
      Infiltration Cellular, Focal, Lymphocyte         4 [1.0]                                                          5 [1.0]     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   Heart                                              (15)         (15)         (15)         (15)         (15)         (15)         
      Myocardium, Necrosis, Focal                                                1 [1.0]                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (15)         (15)         (13)         (15)         (15)         (15)         
      Accessory Adrenal Cortical Nodule                                                                    1 [1.0]                  
      Subcapsular, Hyperplasia, Focal                 14 [1.1]     15 [1.2]     12 [1.4]     15 [1.1]     15 [1.1]     15 [1.1]     
   Thyroid Gland                                      (15)         (15)         (15)         (15)         (15)         (15)         
      Ectopic Thymus                                                                          2 [1.0]                               
      Ultimobranchial Cyst                             1                                                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Average severity grade (1-minimal;2-mild;3-moderate;4-marked)                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page    2                                                              
                                                                                                                                   
NTP Experiment-Test: 99033-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT18  
Study Type: SUBCHRON 26-WEEK                         WITH AVERAGE SEVERITY GRADES[b]                              Date: 03/05/03    
Route: DOSED FEED                                 TRANSGENIC MODEL EVALUATION II (ASPARTAME)                      Time: 11:45:56    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         P16(INK4A)/(+/-) (C57BL/6) FEMALE            VEHICLE      3125 PPM     6250 PPM     12500        25000        50000        
                                                      CONTROL                                PPM          PPM          PPM          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   Tissue NOS                                                                   (1)                                                 
      Abdominal, Fat, Hemorrhage, Focal                                          1 [2.0]                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Ovary                                              (15)         (15)         (15)         (15)         (15)         (15)         
      Cyst                                                                                    1                                     
   Uterus                                             (15)         (15)         (15)         (15)         (15)         (15)         
      Endometrium, Hyperplasia, Cystic                14 [1.9]     13 [1.9]     13 [2.0]     15 [2.1]     15 [1.9]     14 [1.9]     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Lymph Node, Mandibular                             (14)         (15)         (13)         (15)         (15)         (15)         
      Hyperplasia                                                                                                       1 [2.0]     
      Inflammation, Chronic Active                     1 [3.0]                                                                      
   Lymph Node, Mesenteric                             (14)         (15)         (14)         (15)         (15)         (14)         
      Hyperplasia, Histiocytic                                                                                          1 [3.0]     
   Spleen                                             (15)         (15)         (14)         (15)         (15)         (15)         
      Accessory Spleen                                                                        1 [2.0]                               
      Hematopoietic Cell Proliferation                 5 [2.2]      2 [1.5]      4 [1.8]      3 [2.0]      2 [2.0]      3 [2.0]     
      Pigmentation                                     1 [1.0]                                                                      
   Thymus                                             (14)         (15)         (14)         (14)         (15)         (15)         
      Atrophy, Diffuse                                 1 [1.0]                                                                      
      Atrophy, Focal                                                1 [2.0]      1 [2.0]                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   Bone                                               (15)                                                             (15)         
      Cranium, Osteoporosis                            1 [1.0]                                                                      
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (15)         (14)         (14)         (15)         (15)         (15)         
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Average severity grade (1-minimal;2-mild;3-moderate;4-marked)                                                                    
                                                                                                                                    
                                                             Page    3                                                              
                                                                                                                                   
NTP Experiment-Test: 99033-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT18  
Study Type: SUBCHRON 26-WEEK                         WITH AVERAGE SEVERITY GRADES[b]                              Date: 03/05/03    
Route: DOSED FEED                                 TRANSGENIC MODEL EVALUATION II (ASPARTAME)                      Time: 11:45:56    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         P16(INK4A)/(+/-) (C57BL/6) FEMALE            VEHICLE      3125 PPM     6250 PPM     12500        25000        50000        
                                                      CONTROL                                PPM          PPM          PPM          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
NERVOUS SYSTEM - CONT                                                                                                               
      Granuloma                                        1 [2.0]                                                                      
      Cerebellum, Medulla, Vacuolization                                                                                            
        Cytoplasmic                                    1 [1.0]                                                                      
   Spinal Cord                                                                                                         (1)          
      Vacuolization Cytoplasmic                                                                                         1 [3.0]     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (15)         (14)         (14)         (15)         (15)         (15)         
      Alveolar Epithelium, Hyperplasia, Focal          1 [1.0]                                                                      
      Alveolus, Crystals                               1 [4.0]                                                                      
   Nose                                               (15)                                                             (15)         
      Olfactory Epithelium, Atrophy                                                                                     1 [1.0]     
      Olfactory Epithelium, Hyaline Droplet            2 [2.0]                                                          3 [1.7]     
      Respiratory Epithelium, Hyaline Droplet          1 [2.0]                                                          2 [1.5]     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                (14)                                                             (15)         
      Cataract                                         1                                                                            
   Harderian Gland                                    (15)                                                             (15)         
      Hyperplasia                                                                                                       1 [3.0]     
      Infiltration Cellular, Lymphocyte                                                                                 1 [1.0]     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (15)         (15)         (13)         (15)         (15)         (15)         
      Nephropathy                                      1 [1.0]                                                          1 [1.0]     
      Glomerulus, Hyalinization                                                                                         1 [2.0]     
      Renal Tubule, Dilatation, Diffuse                1 [2.0]      1 [2.0]                                1 [2.0]                  
      Renal Tubule, Dilatation, Focal                 10 [1.0]      7 [1.0]      5 [1.0]      5 [1.0]      5 [1.0]      9 [1.0]     
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Average severity grade (1-minimal;2-mild;3-moderate;4-marked)                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page    4                                                              
                                                                                                                                   
NTP Experiment-Test: 99033-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT18  
Study Type: SUBCHRON 26-WEEK                         WITH AVERAGE SEVERITY GRADES[b]                              Date: 03/05/03    
Route: DOSED FEED                                 TRANSGENIC MODEL EVALUATION II (ASPARTAME)                      Time: 11:45:56    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         P16(INK4A)/(+/-) (C57BL/6) MALE              VEHICLE      3125 PPM     6250 PPM     12500        25000        50000        
                                                      CONTROL                                PPM          PPM          PPM          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially In Study                          15           15           15           15           15           15           
  Early Deaths                                                                                                                      
    Natural Death                                      1            1                         1            1                        
  Survivors                                                                                                                         
    Terminal Sacrifice                                14           14           15           14           14           15           
                                                                                                                                    
  Animals Examined Microscopically                    15           15           15           15           15           15           
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Liver                                              (15)         (15)         (15)         (14)         (15)         (15)         
      Infiltration Cellular, Focal, Lymphocyte         3 [1.0]                                                          1 [1.0]     
      Inflammation, Acute                              2 [1.0]                                                                      
      Necrosis, Focal                                  2 [1.5]                   3 [1.0]                   1 [1.0]      4 [1.3]     
      Hepatocyte, Necrosis, Focal                                   1 [1.0]      1 [1.0]                   1 [1.0]      1 [1.0]     
      Hepatocyte, Vacuolization Cytoplasmic,                                                                                        
        Diffuse                                                                               1 [1.0]                               
      Hepatocyte, Periportal, Vacuolization                                                                                         
        Cytoplasmic                                    6 [1.0]     11 [1.5]     14 [1.2]      8 [1.1]     13 [1.1]     13 [1.5]     
   Salivary Glands                                    (15)                                                             (15)         
      Infiltration Cellular, Focal, Lymphocyte        12 [1.0]                                                         11 [1.0]     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (15)         (15)         (15)         (14)         (15)         (15)         
      Accessory Adrenal Cortical Nodule                                                                                 1 [1.0]     
      Subcapsular, Hyperplasia, Focal                  2 [1.0]      1 [1.0]                                1 [1.0]      4 [1.0]     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Average severity grade (1-minimal;2-mild;3-moderate;4-marked)                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page    5                                                              
                                                                                                                                   
NTP Experiment-Test: 99033-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT18  
Study Type: SUBCHRON 26-WEEK                         WITH AVERAGE SEVERITY GRADES[b]                              Date: 03/05/03    
Route: DOSED FEED                                 TRANSGENIC MODEL EVALUATION II (ASPARTAME)                      Time: 11:45:56    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         P16(INK4A)/(+/-) (C57BL/6) MALE              VEHICLE      3125 PPM     6250 PPM     12500        25000        50000        
                                                      CONTROL                                PPM          PPM          PPM          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Epididymis                                         (15)         (15)         (15)         (15)         (15)         (15)         
      Inflammation, Chronic Active, Focal                                        1 [2.0]                                            
      Bilateral, Spermatozoa, Degeneration             1 [1.0]                                                                      
   Testes                                             (15)         (15)         (15)         (14)         (14)         (15)         
      Germinal Epithelium, Degeneration                             1 [2.0]      1 [2.0]                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Lymph Node, Mandibular                             (15)         (15)         (15)         (14)         (14)         (15)         
      Hyperplasia                                      1 [2.0]                                1 [2.0]                               
      Necrosis                                         1 [1.0]                                                                      
   Lymph Node, Mesenteric                             (15)         (15)         (15)         (14)         (14)         (15)         
      Hyperplasia                                                                2 [2.0]                                            
   Spleen                                             (15)         (15)         (15)         (14)         (15)         (15)         
      Atrophy, Diffuse                                 1 [4.0]                                             1 [4.0]                  
      Hematopoietic Cell Proliferation                              1 [2.0]      2 [3.0]      3 [2.0]                   1 [4.0]     
      Pigmentation                                                                                                      1 [1.0]     
      Lymphoid Follicle, Atrophy                       1 [1.0]                                                                      
   Thymus                                             (15)         (14)         (15)         (13)         (15)         (15)         
      Atrophy, Diffuse                                 1 [3.0]                   1 [1.0]                   1 [4.0]                  
      Atrophy, Focal                                   1 [2.0]      2 [1.0]                                1 [1.0]                  
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   Brain                                              (15)         (15)         (15)         (14)         (15)         (15)         
      Cerebellum, Medulla, Vacuolization                                                                                            
        Cytoplasmic                                    1 [1.0]      1 [1.0]                                                         
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Average severity grade (1-minimal;2-mild;3-moderate;4-marked)                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page    6                                                              
                                                                                                                                   
NTP Experiment-Test: 99033-03         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT18  
Study Type: SUBCHRON 26-WEEK                         WITH AVERAGE SEVERITY GRADES[b]                              Date: 03/05/03    
Route: DOSED FEED                                 TRANSGENIC MODEL EVALUATION II (ASPARTAME)                      Time: 11:45:56    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         P16(INK4A)/(+/-) (C57BL/6) MALE              VEHICLE      3125 PPM     6250 PPM     12500        25000        50000        
                                                      CONTROL                                PPM          PPM          PPM          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (15)         (15)         (15)         (15)         (15)         (15)         
      Infiltration Cellular, Focal, Lymphocyte                      1 [1.0]                                                         
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Harderian Gland                                    (15)                                                             (15)         
      Infiltration Cellular, Lymphocyte                2 [1.0]                                                          2 [1.0]     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (15)         (15)         (15)         (14)         (15)         (15)         
      Nephropathy                                      2 [1.0]                                                          7 [1.0]     
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
b  Average severity grade (1-minimal;2-mild;3-moderate;4-marked)                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page    7                                                              
                                                                                                                                   
                             ------------------------------------------------------------                                           
                             ----------              END OF REPORT             ----------                                           
                             ------------------------------------------------------------