National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/19244

TDMS Study 93020-08 Pathology Tables

NTP                                         STATISTICAL ANALYSIS OF SURVIVAL DATA                              REPORT: PEIRPT11     
LAB: Southern Research Inst                             DIISOPROPYLCARBODIIMIDE                                DATE: 07/19/04       
EXPERIMENT: 93020 TEST: 08                                                                                     TIME: 09:19:43       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                   PAGE:       1        
CONT: N01-ES-05451                              ROUTE: SKIN APPLICATION                                        NTP C#: 93020D       
PATHOLOGIST: FARNELL, DANIEL                                                                                   CAS: 693-13-0        
------------------------------------------------------------------------------------------------------------------------------------
                                                                                                                                    
                                                           FINAL #1 MICE                                                            
                                                                                                                                    
                                                                                                                                    
               REASONS FOR REMOVAL:    ALL                                                                                          
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
               REMOVAL DATE RANGE:     ALL                                                                                          
                                                                                                                                    
               TREATMENT GROUPS:       INCLUDE ALL                                                                                  
                                                                                                                                   
NTP                                         STATISTICAL ANALYSIS OF SURVIVAL DATA                              REPORT: PEIRPT11     
LAB: Southern Research Inst                             DIISOPROPYLCARBODIIMIDE                                DATE: 07/19/04       
EXPERIMENT: 93020 TEST: 08                                                                                     TIME: 09:19:43       
TEST TYPE:  CHRONIC                             CAGES FROM 0000 TO LAST CAGE                                                        
CONT: N01-ES-05451                              ROUTE: SKIN APPLICATION                                        NTP C#: 93020D       
PATHOLOGIST: FARNELL, DANIEL                                                                                   CAS: 693-13-0        
                                                                                                                                   
                              DIISOPROPYLCARBODIIMIDE                                                                               
                       STATISTICAL ANALYSIS OF SURVIVAL DATA                                                                        
                                     MALE MICE                                                                                      
                       FIRST TERMINAL SACRIFICE AT 729 DAYS                                                                         
                                                                                                                                    
                          INDIVIDUAL SURVIVAL TIMES (DAYS)                                                                          
 ==================================================================================                                                 
 |                                DOSE = 0 MG/KG                                  |                                                 
 |                             ---------------------                              |                                                 
 |                                                                                |                                                 
 |  TOTAL 50     UNCENSORED DEATHS 11     CENSORED DEATHS 0    TERMINAL 39        |                                                 
 |                                                                                |                                                 
 |  UNCENSORED DEATHS                                                             |                                                 
 |      515   517   592   598   640   672   682   702   706   721                 |                                                 
 |      725                                                                       |                                                 
 |  CENSORED DEATHS                                                               |                                                 
 |      NONE                                                                      |                                                 
 |                                                                                |                                                 
 |--------------------------------------------------------------------------------|                                                 
 |                                DOSE = 10 MG/KG                                 |                                                 
 |                             ---------------------                              |                                                 
 |                                                                                |                                                 
 |  TOTAL 50     UNCENSORED DEATHS 10     CENSORED DEATHS 0    TERMINAL 40        |                                                 
 |                                                                                |                                                 
 |  UNCENSORED DEATHS                                                             |                                                 
 |      54    435   545   603   626   660   670   691   701   710                 |                                                 
 |  CENSORED DEATHS                                                               |                                                 
 |      NONE                                                                      |                                                 
 |                                                                                |                                                 
 |--------------------------------------------------------------------------------|                                                 
 |                                DOSE = 20 MG/KG                                 |                                                 
 |                             ---------------------                              |                                                 
 |                                                                                |                                                 
 |  TOTAL 50     UNCENSORED DEATHS 12     CENSORED DEATHS 0    TERMINAL 38        |                                                 
 |                                                                                |                                                 
 |  UNCENSORED DEATHS                                                             |                                                 
 |      48    55    493   496   597   625   653   669   681   690                 |                                                 
 |      713   715                                                                 |                                                 
 |  CENSORED DEATHS                                                               |                                                 
 |      NONE                                                                      |                                                 
 |                                                                                |                                                 
 ==================================================================================                                                 
                                                                                                                                   
                              DIISOPROPYLCARBODIIMIDE                                                                               
                       STATISTICAL ANALYSIS OF SURVIVAL DATA                                                                        
                                     MALE MICE                                                                                      
                       FIRST TERMINAL SACRIFICE AT 729 DAYS                                                                         
                                                                                                                                    
                          INDIVIDUAL SURVIVAL TIMES (DAYS)                                                                          
 ==================================================================================                                                 
 |                                DOSE = 40 MG/KG                                 |                                                 
 |                             ---------------------                              |                                                 
 |                                                                                |                                                 
 |  TOTAL 50     UNCENSORED DEATHS 13     CENSORED DEATHS 1    TERMINAL 36        |                                                 
 |                                                                                |                                                 
 |  UNCENSORED DEATHS                                                             |                                                 
 |      48    474   484   498   558   573   642   664   667   674                 |                                                 
 |      679   710   712                                                           |                                                 
 |  CENSORED DEATHS                                                               |                                                 
 |      31                                                                        |                                                 
 |                                                                                |                                                 
 ==================================================================================                                                 
                                                                                                                                   
                              DIISOPROPYLCARBODIIMIDE                                                                               
                       STATISTICAL ANALYSIS OF SURVIVAL DATA                                                                        
                                     MALE MICE                                                                                      
                       FIRST TERMINAL SACRIFICE AT 729 DAYS                                                                         
 ==================================================================================                                                 
 |               KAPLAN-MEIER SURVIVAL PROBABILITY ESTIMATES (%)                  |                                                 
 |                                                                                |                                                 
 |--------------------------------------------------------------------------------|                                                 
 |    DOSE                               TIME (DAYS)                              |                                                 
 |                 90   180   270   365   425   485   545   605   665   729 (A)   |                                                 
 |                                                                                |                                                 
 |--------------------------------------------------------------------------------|                                                 
 |           |                                                                    |                                                 
 |0 MG/KG    |   100.0 100.0 100.0 100.0 100.0 100.0  96.0  92.0  90.0  78.0      |                                                 
 |           |                                                                    |                                                 
 |           |                                                                    |                                                 
 |10 MG/KG   |    98.0  98.0  98.0  98.0  98.0  96.0  94.0  92.0  88.0  80.0      |                                                 
 |           |                                                                    |                                                 
 |           |                                                                    |                                                 
 |20 MG/KG   |    96.0  96.0  96.0  96.0  96.0  96.0  92.0  90.0  86.0  76.0      |                                                 
 |           |                                                                    |                                                 
 |           |                                                                    |                                                 
 |40 MG/KG   |    98.0  98.0  98.0  98.0  98.0  93.9  91.8  87.8  83.7  73.5      |                                                 
 |           |                                                                    |                                                 
 |           |                                                                    |                                                 
 ==================================================================================                                                 
 ==================================================================================                                                 
 |                           SURVIVAL SUMMARY STATISTICS                          |                                                 
 |                                                                                |                                                 
 |                                                                                |                                                 
 |--------------------------------------------------------------------------------|                                                 
 |                                                  DOSE                          |                                                 
 |                                                                                |                                                 
 |                                0 MG/KG      10 MG/KG     20 MG/KG     40 MG/KG |                                                 
 |                                                                                |                                                 
 |--------------------------------------------------------------------------------|                                                 
 |SURVIVAL AT END OF STUDY|       78.0%        80.0%        76.0%        73.5%    |                                                 
 |(KAPLAN-MEIER)          |                                                       |                                                 
 |                        |                                                       |                                                 
 |SIGNIFICANCE (B)        |       P=0.499      P=1.000N     P=0.911      P=0.672  |                                                 
 |(LIFE TABLE)            |                                                       |                                                 
 |                        |                                                       |                                                 
 |MEAN DAY OF             |       642.7        569.5        536.3        567.9    |                                                 
 |NATURAL DEATHS (C)      |       (23.3)       (63.1)       (68.8)       (49.4)   |                                                 
 |(STANDARD ERROR)        |                                                       |                                                 
 |                        |                                                       |                                                 
 |MEAN LIFE SPAN (D)      |       710.0        697.1        682.7        673.2    |                                                 
 |(STANDARD ERROR)        |       ( 7.1)       (15.2)       (19.8)       (20.7)   |                                                 
 |                        |                                                       |                                                 
 ==================================================================================                                                 
                                                                                                                                    
       (A)  FIRST TERMINAL SACRIFICE                                                                                                
       (B)  THE FIRST ENTRY IS THE TREND TEST (TARONE, 1975) RESULT.                                                                
            SUBSEQUENT ENTRIES ARE THE RESULTS OF PAIRWISE TESTS                                                                    
            (COX,1972).  NEGATIVE TRENDS ARE INDICATED BY "N".                                                                      
       (C)  MEAN OF ALL UNCENSORED DEATHS PRIOR TO TERMINAL SACRIFICE                                                               
       (D)  MEAN OF ALL DEATHS (UNCENSORED, CENSORED, TERMINAL SACRIFICE)                                                           
                                                                                                                                   
                              DIISOPROPYLCARBODIIMIDE                                                                               
                       STATISTICAL ANALYSIS OF SURVIVAL DATA                                                                        
                                    FEMALE MICE                                                                                     
                       FIRST TERMINAL SACRIFICE AT 729 DAYS                                                                         
                                                                                                                                    
                          INDIVIDUAL SURVIVAL TIMES (DAYS)                                                                          
 ==================================================================================                                                 
 |                                DOSE = 0 MG/KG                                  |                                                 
 |                             ---------------------                              |                                                 
 |                                                                                |                                                 
 |  TOTAL 50     UNCENSORED DEATHS 17     CENSORED DEATHS 0    TERMINAL 33        |                                                 
 |                                                                                |                                                 
 |  UNCENSORED DEATHS                                                             |                                                 
 |      44    305   365   488   506   521   603   603   619   653                 |                                                 
 |      654   681   683   697   698   715   720                                   |                                                 
 |  CENSORED DEATHS                                                               |                                                 
 |      NONE                                                                      |                                                 
 |                                                                                |                                                 
 |--------------------------------------------------------------------------------|                                                 
 |                                DOSE = 10 MG/KG                                 |                                                 
 |                             ---------------------                              |                                                 
 |                                                                                |                                                 
 |  TOTAL 50     UNCENSORED DEATHS 16     CENSORED DEATHS 1    TERMINAL 33        |                                                 
 |                                                                                |                                                 
 |  UNCENSORED DEATHS                                                             |                                                 
 |      540   555   568   579   588   617   619   625   653   653                 |                                                 
 |      653   683   684   690   695   707                                         |                                                 
 |  CENSORED DEATHS                                                               |                                                 
 |      12                                                                        |                                                 
 |                                                                                |                                                 
 |--------------------------------------------------------------------------------|                                                 
 |                                DOSE = 20 MG/KG                                 |                                                 
 |                             ---------------------                              |                                                 
 |                                                                                |                                                 
 |  TOTAL 50     UNCENSORED DEATHS 10     CENSORED DEATHS 1    TERMINAL 39        |                                                 
 |                                                                                |                                                 
 |  UNCENSORED DEATHS                                                             |                                                 
 |      67    417   560   579   584   603   614   653   687   690                 |                                                 
 |  CENSORED DEATHS                                                               |                                                 
 |      15                                                                        |                                                 
 |                                                                                |                                                 
 ==================================================================================                                                 
                                                                                                                                   
                              DIISOPROPYLCARBODIIMIDE                                                                               
                       STATISTICAL ANALYSIS OF SURVIVAL DATA                                                                        
                                    FEMALE MICE                                                                                     
                       FIRST TERMINAL SACRIFICE AT 729 DAYS                                                                         
                                                                                                                                    
                          INDIVIDUAL SURVIVAL TIMES (DAYS)                                                                          
 ==================================================================================                                                 
 |                                DOSE = 40 MG/KG                                 |                                                 
 |                             ---------------------                              |                                                 
 |                                                                                |                                                 
 |  TOTAL 50     UNCENSORED DEATHS 9      CENSORED DEATHS 1    TERMINAL 40        |                                                 
 |                                                                                |                                                 
 |  UNCENSORED DEATHS                                                             |                                                 
 |      481   528   551   582   604   617   619   669   718                       |                                                 
 |  CENSORED DEATHS                                                               |                                                 
 |      105                                                                       |                                                 
 |                                                                                |                                                 
 ==================================================================================                                                 
                                                                                                                                   
                              DIISOPROPYLCARBODIIMIDE                                                                               
                       STATISTICAL ANALYSIS OF SURVIVAL DATA                                                                        
                                    FEMALE MICE                                                                                     
                       FIRST TERMINAL SACRIFICE AT 729 DAYS                                                                         
 ==================================================================================                                                 
 |               KAPLAN-MEIER SURVIVAL PROBABILITY ESTIMATES (%)                  |                                                 
 |                                                                                |                                                 
 |--------------------------------------------------------------------------------|                                                 
 |    DOSE                               TIME (DAYS)                              |                                                 
 |                 90   180   270   365   425   485   545   605   665   729 (A)   |                                                 
 |                                                                                |                                                 
 |--------------------------------------------------------------------------------|                                                 
 |           |                                                                    |                                                 
 |0 MG/KG    |    98.0  98.0  98.0  94.0  94.0  94.0  88.0  84.0  78.0  66.0      |                                                 
 |           |                                                                    |                                                 
 |           |                                                                    |                                                 
 |10 MG/KG   |   100.0 100.0 100.0 100.0 100.0 100.0  98.0  89.8  77.6  67.3      |                                                 
 |           |                                                                    |                                                 
 |           |                                                                    |                                                 
 |20 MG/KG   |    98.0  98.0  98.0  98.0  95.9  95.9  95.9  87.8  83.7  79.6      |                                                 
 |           |                                                                    |                                                 
 |           |                                                                    |                                                 
 |40 MG/KG   |   100.0 100.0 100.0 100.0 100.0  98.0  95.9  89.8  85.7  81.6      |                                                 
 |           |                                                                    |                                                 
 |           |                                                                    |                                                 
 ==================================================================================                                                 
 ==================================================================================                                                 
 |                           SURVIVAL SUMMARY STATISTICS                          |                                                 
 |                                                                                |                                                 
 |                                                                                |                                                 
 |--------------------------------------------------------------------------------|                                                 
 |                                                  DOSE                          |                                                 
 |                                                                                |                                                 
 |                                0 MG/KG      10 MG/KG     20 MG/KG     40 MG/KG |                                                 
 |                                                                                |                                                 
 |--------------------------------------------------------------------------------|                                                 
 |SURVIVAL AT END OF STUDY|       66.0%        67.3%        79.6%        81.6%    |                                                 
 |(KAPLAN-MEIER)          |                                                       |                                                 
 |                        |                                                       |                                                 
 |SIGNIFICANCE (B)        |       P=0.065N     P=0.985N     P=0.211N     P=0.125N |                                                 
 |(LIFE TABLE)            |                                                       |                                                 
 |                        |                                                       |                                                 
 |MEAN DAY OF             |       562.1        631.8        545.4        596.6    |                                                 
 |NATURAL DEATHS (C)      |       (43.8)       (13.4)       (58.6)       (24.0)   |                                                 
 |(STANDARD ERROR)        |                                                       |                                                 
 |                        |                                                       |                                                 
 |MEAN LIFE SPAN (D)      |       672.2        683.6        678.0        692.7    |                                                 
 |(STANDARD ERROR)        |       (18.4)       (15.7)       (20.5)       (14.6)   |                                                 
 |                        |                                                       |                                                 
 ==================================================================================                                                 
                                                                                                                                    
       (A)  FIRST TERMINAL SACRIFICE                                                                                                
       (B)  THE FIRST ENTRY IS THE TREND TEST (TARONE, 1975) RESULT.                                                                
            SUBSEQUENT ENTRIES ARE THE RESULTS OF PAIRWISE TESTS                                                                    
            (COX,1972).  NEGATIVE TRENDS ARE INDICATED BY "N".                                                                      
       (C)  MEAN OF ALL UNCENSORED DEATHS PRIOR TO TERMINAL SACRIFICE                                                               
       (D)  MEAN OF ALL DEATHS (UNCENSORED, CENSORED, TERMINAL SACRIFICE)                                                           
                                                                                                                                   
                                                      DIISOPROPYLCARBODIIMIDE                                                       
                                                    KAPLAN-MEIER SURVIVAL CURVES                                                    
                                                        0 MG/KG          = 1                                                        
                                                        10 MG/KG         = 2                                                        
                                                        20 MG/KG         = 3                                                        
                                                        40 MG/KG         = 4                                                        
                                                                                                                                    
------------------------------------------------------ SPECIES=MICE SEX=MALE -------------------------------------------------------
                                                                                                                                    
                                         Plot of SURVIVAL*WEEK.  Symbol is value of DOSE.                                           
                                                                                                                                    
      |                                                                                                                             
      |                                                                                                                             
  100 + 1       32                                                                       1                                          
      |                                                                                                                             
      |         32                                                           2     4                                                
      |          3                                                           2     4 43  1    2       1                             
P  95 +                                                                                                                             
E     |                                                                              4 4      2       112                           
R     |                                                                               3                12  2 1                      
C     |                                                                                4        4                                   
E  90 +                                                                                         4 4    3   2 1   21                 
N     |                                                                                                                             
T     |                                                                                           4        3 4 3 21 1               
      |                                                                                                      4 3 42 12 1            
S  85 +                                                                                                                             
U     |                                                                                                          43 32 2            
R     |                                                                                                             33 121          
V     |                                                                                                           44                
I  80 +                                                                                                              3  211 2       
V     |                                                                                                                             
A     |                                                                                                            4    331 1       
L     |                                                                                                                  3  3       
   75 +                                                                                                                             
      |                                                                                                                 4   4       
      |                                                                                                                             
      |                                                                                                                             
   70 +                                                                                                                             
      |                                                                                                                             
      --+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+--
        0         10         20         30         40         50         60         70         80         90         100        110 
                                                                                                                                    
                                                                   WEEK                                                             
                                                                                                                                    
NOTE: 14 obs hidden.                                                                                                                
                                                                                                                                   
                                                      DIISOPROPYLCARBODIIMIDE                                                       
                                                    KAPLAN-MEIER SURVIVAL CURVES                                                    
                                                        0 MG/KG          = 1                                                        
                                                        10 MG/KG         = 2                                                        
                                                        20 MG/KG         = 3                                                        
                                                        40 MG/KG         = 4                                                        
                                                                                                                                    
----------------------------------------------------- SPECIES=MICE SEX=FEMALE ------------------------------------------------------
                                                                                                                                    
                                         Plot of SURVIVAL*WEEK.  Symbol is value of DOSE.                                           
                                                                                                                                    
      |                                                                                                                             
      |                                                                                                                             
  100 + 1       1  3                                                                4         2                                     
      |                                                                                                                             
      |         1  3                                    1                 3         4       4 2 2                                   
      |                                                 1         1       3                 4  42 2                                 
   95 +                                                                                                                             
      |                                                           1                  1         43 224                               
P     |                                                                              1  1                                           
E     |                                                                                            22   4                           
R  90 +                                                                                 1  1        2   3 2                         
C     |                                                                                                                             
E     |                                                                                    1            13                          
N     |                                                                                                  322   3  4                 
T  85 +                                                                                                                             
      |                                                                                                 1 12   2  4  3   4          
S     |                                                                                                   1    1                    
U     |                                                                                                                  4  4       
R  80 +                                                                                                              3      3       
V     |                                                                                                                             
I     |                                                                                                        1    1               
V     |                                                                                                                             
A  75 +                                                                                                                             
L     |                                                                                                             121             
      |                                                                                                                             
      |                                                                                                              22             
   70 +                                                                                                               12 1          
      |                                                                                                                             
      |                                                                                                                2    2       
      |                                                                                                                  1  1       
   65 +                                                                                                                             
      |                                                                                                                             
      --+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+----------+--
        0         10         20         30         40         50         60         70         80         90         100        110 
                                                                                                                                    
                                                                   WEEK                                                             
                                                                                                                                    
NOTE: 18 obs hidden.                                                                                                                
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.