National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/20463

TDMS Study 97013-06 Pathology Tables

NTP Experiment-Test: 97013-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: SUBCHRON 26-WEEK                 WATER DISINFECTION MODEL (BROMODICHLOROMETHANE)                      Date: 06/11/04
Route: SKIN APPLICATION                                                                                           Time: 08:38:10

                                                          FINAL#1 MICE




       Facility:  Battelle Columbus Laboratory

       Chemical CAS #:  75-27-4

       Lock Date:  07/19/01

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include 002    0 MG/KG
                               Include 004    64 MG/KG
                               Include 006    128     MG/KG
                               Include 008    256     MG/KG
                               Include 001    0 MG/KG
                               Include 003    64 MG/KG
                               Include 005    128     MG/KG
                               Include 007    256     MG/KG



























a  Number of animals examined microscopically at site and number of animals with lesion

                                                              Page   1


NTP Experiment-Test: 97013-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: SUBCHRON 26-WEEK                 WATER DISINFECTION MODEL (BROMODICHLOROMETHANE)                      Date: 06/11/04    
Route: SKIN APPLICATION                                                                                           Time: 08:38:10    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         TGAC (FVB/N) HEMIZYGOUS FEMALE            0 MG/KG      64 MG/KG     128          256                                       
                                                                             MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially In Study                          15           15           15           15                                     
  Early Deaths                                                                                                                      
    Moribund Sacrifice                                 2            3            1            4                                     
    Natural Death                                      2            2            2            1                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                11           10           12           10                                     
                                                                                                                                    
  Animals Examined Microscopically                    15           15           15           15                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Liver                                              (15)         (15)         (15)         (15)                                   
      Hematopoietic Cell Proliferation                 1 (7%)       1 (7%)       1 (7%)       3 (20%)                               
      Inflammation                                    14 (93%)     11 (73%)     14 (93%)     11 (73%)                               
      Necrosis                                         3 (20%)      2 (13%)      4 (27%)      2 (13%)                               
      Hepatocyte, Vacuolization Cytoplasmic            3 (20%)      7 (47%)      5 (33%)      5 (33%)                               
   Mesentery                                          (1)                                                                           
      Necrosis                                         1 (100%)                                                                     
   Stomach, Forestomach                               (15)         (15)         (15)         (15)                                   
      Inflammation                                                               1 (7%)                                             
      Epithelium, Hyperkeratosis                       1 (7%)                                                                       
      Epithelium, Hyperplasia                          1 (7%)                                                                       
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (15)         (15)         (15)         (15)                                   
      Subcapsular, Hyperplasia                         8 (53%)      8 (53%)     11 (73%)      5 (33%)                               
   Pituitary Gland                                    (15)         (15)         (15)         (15)                                   
      Cyst                                             4 (27%)      1 (7%)       1 (7%)       2 (13%)                               
   Thyroid Gland                                      (15)         (15)         (15)         (15)                                   
      Cyst                                             2 (13%)      1 (7%)       1 (7%)       1 (7%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    2                                                              
                                                                                                                                   
NTP Experiment-Test: 97013-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: SUBCHRON 26-WEEK                 WATER DISINFECTION MODEL (BROMODICHLOROMETHANE)                      Date: 06/11/04    
Route: SKIN APPLICATION                                                                                           Time: 08:38:10    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         TGAC (FVB/N) HEMIZYGOUS FEMALE            0 MG/KG      64 MG/KG     128          256                                       
                                                                             MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Ovary                                              (15)         (14)         (15)         (15)                                   
      Cyst                                             1 (7%)                                 2 (13%)                               
   Uterus                                             (15)         (15)         (15)         (15)                                   
      Inflammation, Suppurative                        2 (13%)                   1 (7%)                                             
      Endometrium, Hyperplasia, Cystic                 7 (47%)      6 (40%)     10 (67%)      6 (40%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Lymph Node                                         (1)                                                                           
      Mediastinal, Hyperplasia                         1 (100%)                                                                     
   Lymph Node, Mandibular                             (15)         (15)         (15)         (15)                                   
      Hematopoietic Cell Proliferation                                                        1 (7%)                                
      Infiltration Cellular, Plasma Cell                            1 (7%)       1 (7%)       1 (7%)                                
   Spleen                                             (15)         (15)         (15)         (15)                                   
      Hematopoietic Cell Proliferation                 2 (13%)      1 (7%)       1 (7%)       4 (27%)                               
   Thymus                                             (15)         (15)         (15)         (14)                                   
      Atrophy                                                       3 (20%)      1 (7%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Skin                                               (15)         (15)         (15)         (15)                                   
      Epidermis, Hyperplasia, Focal                                              1 (7%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (15)         (15)         (15)         (15)                                   
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    3                                                              
                                                                                                                                   
NTP Experiment-Test: 97013-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: SUBCHRON 26-WEEK                 WATER DISINFECTION MODEL (BROMODICHLOROMETHANE)                      Date: 06/11/04    
Route: SKIN APPLICATION                                                                                           Time: 08:38:10    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         TGAC (FVB/N) HEMIZYGOUS FEMALE            0 MG/KG      64 MG/KG     128          256                                       
                                                                             MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
RESPIRATORY SYSTEM - CONT                                                                                                           
      Inflammation                                     1 (7%)                                                                       
      Alveolar Epithelium, Hyperplasia                 1 (7%)                                                                       
      Artery, Inflammation                                          1 (7%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   Eye                                                             (1)                       (1)                                    
      Retina, Degeneration                                          1 (100%)                  1 (100%)                              
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (15)         (15)         (15)         (15)                                   
      Casts Protein                                    6 (40%)      5 (33%)      5 (33%)      4 (27%)                               
      Cyst                                             2 (13%)      2 (13%)                   1 (7%)                                
      Hydronephrosis                                                             1 (7%)                                             
      Mineralization                                                1 (7%)                                                          
      Nephropathy                                                                2 (13%)      3 (20%)                               
      Artery, Inflammation                                          1 (7%)                    1 (7%)                                
      Renal Tubule, Hypertrophy                                     1 (7%)       1 (7%)       4 (27%)                               
      Renal Tubule, Necrosis                           1 (7%)                                                                       
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page    4                                                              
                                                                                                                                   
NTP Experiment-Test: 97013-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: SUBCHRON 26-WEEK                 WATER DISINFECTION MODEL (BROMODICHLOROMETHANE)                      Date: 06/11/04    
Route: SKIN APPLICATION                                                                                           Time: 08:38:10    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         TGAC (FVB/N) HEMIZYGOUS MALE              0 MG/KG      64 MG/KG     128          256                                       
                                                                             MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially In Study                          15           15           15           15                                     
  Early Deaths                                                                                                                      
    Moribund Sacrifice                                 2            1                                                               
    Natural Death                                                                             2                                     
  Survivors                                                                                                                         
    Terminal Sacrifice                                13           14           15           13                                     
                                                                                                                                    
  Animals Examined Microscopically                    15           15           15           15                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Liver                                              (15)         (15)         (15)         (15)                                   
      Hematopoietic Cell Proliferation                 1 (7%)       1 (7%)                    1 (7%)                                
      Inflammation                                     8 (53%)      8 (53%)      5 (33%)      6 (40%)                               
      Necrosis                                         1 (7%)       1 (7%)       1 (7%)       2 (13%)                               
      Hepatocyte, Fatty Change                         1 (7%)                                                                       
      Hepatocyte, Vacuolization Cytoplasmic            4 (27%)      2 (13%)      5 (33%)      4 (27%)                               
   Mesentery                                                                    (1)                                                 
      Fat, Necrosis                                                              1 (100%)                                           
   Stomach, Forestomach                               (15)         (15)         (15)         (15)                                   
      Hyperkeratosis                                                                          1 (7%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (15)         (15)         (15)         (15)                                   
      Hyperplasia                                      1 (7%)                                                                       
      Hypertrophy                                      9 (60%)      9 (60%)      6 (40%)      8 (53%)                               
      Vacuolization Cytoplasmic                                                               1 (7%)                                
      Subcapsular, Hyperplasia                         1 (7%)       1 (7%)                    2 (13%)                               
   Thyroid Gland                                      (15)         (15)         (15)         (15)                                   
      Cyst                                             1 (7%)                    2 (13%)                                            
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    5                                                              
                                                                                                                                   
NTP Experiment-Test: 97013-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: SUBCHRON 26-WEEK                 WATER DISINFECTION MODEL (BROMODICHLOROMETHANE)                      Date: 06/11/04    
Route: SKIN APPLICATION                                                                                           Time: 08:38:10    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         TGAC (FVB/N) HEMIZYGOUS MALE              0 MG/KG      64 MG/KG     128          256                                       
                                                                             MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Testes                                             (15)         (15)         (15)         (15)                                   
      Germinal Epithelium, Degeneration                3 (20%)      1 (7%)       2 (13%)      1 (7%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Lymph Node, Mandibular                             (15)         (15)         (14)         (14)                                   
      Atrophy                                                                                 1 (7%)                                
      Infiltration Cellular, Plasma Cell               1 (7%)                                                                       
   Lymph Node, Mesenteric                             (14)         (15)         (15)         (15)                                   
      Atrophy                                                       1 (7%)                                                          
   Spleen                                             (15)         (15)         (15)         (15)                                   
      Hematopoietic Cell Proliferation                 1 (7%)       2 (13%)      1 (7%)       1 (7%)                                
      Lymphoid Follicle, Atrophy                                                              1 (7%)                                
      Lymphoid Follicle, Hyperplasia                                                          1 (7%)                                
   Thymus                                             (15)         (15)         (15)         (15)                                   
      Atrophy                                          1 (7%)       1 (7%)                    2 (13%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   Skin                                               (15)         (15)         (15)         (15)                                   
      Epidermis, Hyperplasia, Focal                    1 (7%)                                                                       
      Subcutaneous Tissue, Inflammation                             1 (7%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (15)         (15)         (15)         (15)                                   
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    6                                                              
                                                                                                                                   
NTP Experiment-Test: 97013-06         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: SUBCHRON 26-WEEK                 WATER DISINFECTION MODEL (BROMODICHLOROMETHANE)                      Date: 06/11/04    
Route: SKIN APPLICATION                                                                                           Time: 08:38:10    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         TGAC (FVB/N) HEMIZYGOUS MALE              0 MG/KG      64 MG/KG     128          256                                       
                                                                             MG/KG        MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
RESPIRATORY SYSTEM - CONT                                                                                                           
      Inflammation                                                  1 (7%)       1 (7%)                                             
      Thrombosis                                                                 1 (7%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (15)         (15)         (15)         (15)                                   
      Casts Protein                                    3 (20%)      1 (7%)                    2 (13%)                               
      Cyst                                             1 (7%)                                 1 (7%)                                
      Hydronephrosis                                   1 (7%)                                 1 (7%)                                
      Inflammation                                                                            1 (7%)                                
      Nephropathy                                      7 (47%)      6 (40%)      7 (47%)      6 (40%)                               
      Artery, Inflammation                             1 (7%)                    1 (7%)                                             
      Renal Tubule, Dilatation                         1 (7%)                                 1 (7%)                                
      Renal Tubule, Hypertrophy                                                               1 (7%)                                
      Renal Tubule, Necrosis                                                                  1 (7%)                                
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page    7                                                              
                                                                                                                                   
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                             ----------              END OF REPORT             ----------                                           
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