National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/20691

TDMS Study 97013-08 Pathology Tables

NTP Experiment-Test: 97013-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03
Study Type: SUBCHRON 26-WEEK                 WATER DISINFECTION MODEL (BROMODICHLOROMETHANE)                      Date: 06/11/04
Route: GAVAGE                                                                                                     Time: 08:45:50

                                                          FINAL#1 MICE




       Facility:  Battelle Columbus Laboratory

       Chemical CAS #:  75-27-4

       Lock Date:  07/19/01

       Cage Range:  All

       Reasons For Removal:    All

       Removal Date Range:     All

       Treatment Groups:       Include 002    0 MG/KG
                               Include 004    25 MG/KG
                               Include 006    50 MG/KG
                               Include 008    100     MG/KG
                               Include 001    0 MG/KG
                               Include 003    25 MG/KG
                               Include 005    50 MG/KG
                               Include 007    100     MG/KG



























a  Number of animals examined microscopically at site and number of animals with lesion

                                                              Page   1


NTP Experiment-Test: 97013-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: SUBCHRON 26-WEEK                 WATER DISINFECTION MODEL (BROMODICHLOROMETHANE)                      Date: 06/11/04    
Route: GAVAGE                                                                                                     Time: 08:45:50    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         TGAC (FVB/N) HEMIZYGOUS FEMALE            0 MG/KG      25 MG/KG     50 MG/KG     100                                       
                                                                                          MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially In Study                          15           15           15           15                                     
  Early Deaths                                                                                                                      
    Moribund Sacrifice                                 1                                      1                                     
    Dosing Accident                                    1                                      1                                     
    Natural Death                                      2            1            2                                                  
  Survivors                                                                                                                         
    Terminal Sacrifice                                11           14           13           13                                     
                                                                                                                                    
  Animals Examined Microscopically                    15           15           15           15                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Esophagus                                          (1)                                    (1)                                    
      Perforation                                      1 (100%)                               1 (100%)                              
   Liver                                              (15)         (15)         (15)         (15)                                   
      Basophilic Focus                                 1 (7%)                                                                       
      Cyst                                                                       1 (7%)                                             
      Hematopoietic Cell Proliferation                 1 (7%)                    1 (7%)       1 (7%)                                
      Hemorrhage                                                    1 (7%)                                                          
      Hepatodiaphragmatic Nodule                                    1 (7%)                                                          
      Inflammation                                    11 (73%)     13 (87%)     13 (87%)     13 (87%)                               
      Necrosis                                         1 (7%)                    4 (27%)      1 (7%)                                
      Hepatocyte, Fatty Change                                      5 (33%)      8 (53%)      7 (47%)                               
      Hepatocyte, Vacuolization Cytoplasmic                         6 (40%)      4 (27%)      3 (20%)                               
   Salivary Glands                                    (1)                                    (1)                                    
      Atrophy                                                                                 1 (100%)                              
   Stomach, Forestomach                               (15)         (15)         (15)         (15)                                   
      Hyperkeratosis                                   1 (7%)                                                                       
      Epithelium, Hyperplasia                                       1 (7%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (15)         (15)         (15)         (15)                                   
      Mineralization                                                1 (7%)                                                          
      Subcapsular, Hyperplasia                         7 (47%)     10 (67%)      8 (53%)      6 (40%)                               
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    2                                                              
                                                                                                                                   
NTP Experiment-Test: 97013-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: SUBCHRON 26-WEEK                 WATER DISINFECTION MODEL (BROMODICHLOROMETHANE)                      Date: 06/11/04    
Route: GAVAGE                                                                                                     Time: 08:45:50    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         TGAC (FVB/N) HEMIZYGOUS FEMALE            0 MG/KG      25 MG/KG     50 MG/KG     100                                       
                                                                                          MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ENDOCRINE SYSTEM - CONT                                                                                                             
   Parathyroid Gland                                               (1)                                                              
      Cyst                                                          1 (100%)                                                        
   Pituitary Gland                                    (15)         (15)         (15)         (15)                                   
      Angiectasis                                                                1 (7%)                                             
      Cyst                                             1 (7%)                    1 (7%)       2 (13%)                               
   Thyroid Gland                                      (15)         (15)         (15)         (15)                                   
      Cyst                                             2 (13%)      2 (13%)      3 (20%)      1 (7%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Ovary                                              (15)         (15)         (15)         (15)                                   
      Atrophy                                                                                 1 (7%)                                
      Cyst                                             4 (27%)      2 (13%)      4 (27%)      4 (27%)                               
   Uterus                                             (15)         (15)         (15)         (15)                                   
      Atrophy                                                                                 1 (7%)                                
      Inflammation                                                  1 (7%)                                                          
      Endometrium, Hyperplasia, Cystic                12 (80%)      9 (60%)     12 (80%)     13 (87%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Lymph Node, Mandibular                             (14)         (15)         (15)         (15)                                   
      Hyperplasia                                                                             1 (7%)                                
      Infiltration Cellular, Plasma Cell               1 (7%)                                 1 (7%)                                
   Lymph Node, Mesenteric                             (14)         (15)         (15)         (15)                                   
      Atrophy                                                       1 (7%)                                                          
   Spleen                                             (15)         (15)         (15)         (15)                                   
      Hematopoietic Cell Proliferation                                           2 (13%)      1 (7%)                                
      Lymphoid Follicle, Atrophy                       3 (20%)                                1 (7%)                                
   Thymus                                             (13)         (15)         (15)         (15)                                   
      Atrophy                                          2 (15%)      3 (20%)      1 (7%)       3 (20%)                               
      Cyst                                             2 (15%)      5 (33%)      5 (33%)      2 (13%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   None                                                                                                                             
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    3                                                              
                                                                                                                                   
NTP Experiment-Test: 97013-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: SUBCHRON 26-WEEK                 WATER DISINFECTION MODEL (BROMODICHLOROMETHANE)                      Date: 06/11/04    
Route: GAVAGE                                                                                                     Time: 08:45:50    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         TGAC (FVB/N) HEMIZYGOUS FEMALE            0 MG/KG      25 MG/KG     50 MG/KG     100                                       
                                                                                          MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (15)         (15)         (15)         (15)                                   
      Inflammation                                                               2 (13%)                                            
      Thrombosis                                                                              1 (7%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (15)         (15)         (15)         (15)                                   
      Casts Protein                                    4 (27%)      5 (33%)      5 (33%)      4 (27%)                               
      Cyst                                                                       1 (7%)       2 (13%)                               
      Mineralization                                                                          1 (7%)                                
      Nephropathy                                      1 (7%)       3 (20%)      3 (20%)      1 (7%)                                
      Renal Tubule, Degeneration                                                 1 (7%)                                             
      Renal Tubule, Dilatation                         1 (7%)                                                                       
      Renal Tubule, Hypertrophy                        1 (7%)       1 (7%)       1 (7%)       8 (53%)                               
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page    4                                                              
                                                                                                                                   
NTP Experiment-Test: 97013-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: SUBCHRON 26-WEEK                 WATER DISINFECTION MODEL (BROMODICHLOROMETHANE)                      Date: 06/11/04    
Route: GAVAGE                                                                                                     Time: 08:45:50    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         TGAC (FVB/N) HEMIZYGOUS MALE              0 MG/KG      25 MG/KG     50 MG/KG     100                                       
                                                                                          MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
DISPOSITION SUMMARY                                                                                                                 
                                                                                                                                    
  Animals Initially In Study                          15           15           15           15                                     
  Early Deaths                                                                                                                      
    Dosing Accident                                    1                                                                            
    Moribund Sacrifice                                 1            1            3                                                  
  Survivors                                                                                                                         
    Terminal Sacrifice                                13           14           12           14                                     
    Natural Death                                                                             1                                     
                                                                                                                                    
  Animals Examined Microscopically                    15           15           15           15                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ALIMENTARY SYSTEM                                                                                                                  
                                                                                                                                    
   Esophagus                                          (1)                                                                           
      Inflammation                                     1 (100%)                                                                     
   Liver                                              (15)         (15)         (15)         (15)                                   
      Cyst                                                                                    1 (7%)                                
      Hematopoietic Cell Proliferation                              1 (7%)                                                          
      Inflammation                                     6 (40%)      7 (47%)      9 (60%)      8 (53%)                               
      Necrosis                                         1 (7%)                    1 (7%)                                             
      Hepatocyte, Fatty Change                         1 (7%)                                                                       
      Hepatocyte, Vacuolization Cytoplasmic            3 (20%)      1 (7%)       3 (20%)      3 (20%)                               
   Mesentery                                          (1)                       (1)                                                 
      Fat, Necrosis                                    1 (100%)                  1 (100%)                                           
   Stomach, Forestomach                               (15)         (15)         (15)         (15)                                   
      Hyperkeratosis                                   1 (7%)                                                                       
   Tooth                                              (3)          (2)          (3)          (3)                                    
      Inflammation                                                                            1 (33%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 CARDIOVASCULAR SYSTEM                                                                                                              
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 ENDOCRINE SYSTEM                                                                                                                   
                                                                                                                                    
   Adrenal Cortex                                     (15)         (15)         (15)         (15)                                   
      Hypertrophy                                      6 (40%)      8 (53%)      5 (33%)      7 (47%)                               
      Subcapsular, Hyperplasia                                      2 (13%)                                                         
   Pituitary Gland                                    (15)         (15)         (15)         (15)                                   
      Cyst                                             2 (13%)      2 (13%)      1 (7%)       1 (7%)                                
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    5                                                              
                                                                                                                                   
NTP Experiment-Test: 97013-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: SUBCHRON 26-WEEK                 WATER DISINFECTION MODEL (BROMODICHLOROMETHANE)                      Date: 06/11/04    
Route: GAVAGE                                                                                                     Time: 08:45:50    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         TGAC (FVB/N) HEMIZYGOUS MALE              0 MG/KG      25 MG/KG     50 MG/KG     100                                       
                                                                                          MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
ENDOCRINE SYSTEM - CONT                                                                                                             
   Thyroid Gland                                      (15)         (15)         (15)         (15)                                   
      Cyst                                             2 (13%)      2 (13%)                   1 (7%)                                
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENERAL BODY SYSTEM                                                                                                                
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 GENITAL SYSTEM                                                                                                                     
                                                                                                                                    
   Epididymis                                         (15)         (15)         (15)         (15)                                   
      Inflammation                                                               1 (7%)                                             
   Preputial Gland                                                              (2)                                                 
      Cyst                                                                       1 (50%)                                            
      Duct, Ectasia                                                              1 (50%)                                            
   Testes                                             (15)         (15)         (15)         (15)                                   
      Germinal Epithelium, Degeneration                1 (7%)       4 (27%)      1 (7%)                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 HEMATOPOIETIC SYSTEM                                                                                                               
                                                                                                                                    
   Lymph Node, Mandibular                             (15)         (15)         (15)         (15)                                   
      Atrophy                                                                                 1 (7%)                                
      Hyperplasia                                      1 (7%)                                 2 (13%)                               
      Infiltration Cellular, Plasma Cell                            2 (13%)                                                         
   Lymph Node, Mesenteric                             (15)         (14)         (15)         (15)                                   
      Atrophy                                          1 (7%)                                 1 (7%)                                
   Spleen                                             (15)         (15)         (15)         (15)                                   
      Hematopoietic Cell Proliferation                 1 (7%)       1 (7%)       2 (13%)                                            
      Lymphoid Follicle, Atrophy                       1 (7%)                    1 (7%)                                             
   Thymus                                             (14)         (15)         (15)         (15)                                   
      Atrophy                                          2 (14%)      1 (7%)       3 (20%)      2 (13%)                               
      Cyst                                             7 (50%)      5 (33%)      4 (27%)      8 (53%)                               
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 INTEGUMENTARY SYSTEM                                                                                                               
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 MUSCULOSKELETAL SYSTEM                                                                                                             
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                             Page    6                                                              
                                                                                                                                   
NTP Experiment-Test: 97013-08         INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a)               Report: PEIRPT03  
Study Type: SUBCHRON 26-WEEK                 WATER DISINFECTION MODEL (BROMODICHLOROMETHANE)                      Date: 06/11/04    
Route: GAVAGE                                                                                                     Time: 08:45:50    
____________________________________________________________________________________________________________________________________
                                                                                                                                    
         TGAC (FVB/N) HEMIZYGOUS MALE              0 MG/KG      25 MG/KG     50 MG/KG     100                                       
                                                                                          MG/KG                                     
____________________________________________________________________________________________________________________________________
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 NERVOUS SYSTEM                                                                                                                     
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 RESPIRATORY SYSTEM                                                                                                                 
                                                                                                                                    
   Lung                                               (15)         (15)         (15)         (15)                                   
      Edema                                                         1 (7%)                                                          
      Inflammation                                                                            1 (7%)                                
      Alveolar Epithelium, Hyperplasia                              1 (7%)                                                          
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 SPECIAL SENSES SYSTEM                                                                                                              
                                                                                                                                    
   None                                                                                                                             
____________________________________________________________________________________________________________________________________
                                                                                                                                    
 URINARY SYSTEM                                                                                                                     
                                                                                                                                    
   Kidney                                             (15)         (15)         (15)         (15)                                   
      Casts Protein                                    3 (20%)                                6 (40%)                               
      Cyst                                                          1 (7%)                                                          
      Hydronephrosis                                                1 (7%)                                                          
      Nephropathy                                      2 (13%)      8 (53%)      3 (20%)      4 (27%)                               
      Artery, Inflammation                                          1 (7%)                                                          
      Renal Tubule, Degeneration                                                              4 (27%)                               
      Renal Tubule, Dilatation                         2 (13%)      1 (7%)       5 (33%)      4 (27%)                               
      Renal Tubule, Hypertrophy                                                  4 (27%)      1 (7%)                                
      Renal Tubule, Necrosis                                                                  1 (7%)                                
                                                                                                                                    
                                                                                                                                    
a  Number of animals examined microscopically at site and number of animals with lesion                                             
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                                                                                                    
                                                             Page    7                                                              
                                                                                                                                   
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