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Abstract for TR-44


In Vitro Carcinogenisis: Guide to the Literature, Recent Advances andLaboratory Procedures (1978)

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In the Fall 1974, plans for a "Workshop on In Vitro ChemicalCarcinogenesis" were initiated at the suggestion of Drs. UmbertoSaffiotti and I. Bernard Weinstein and developed together with Dr.Donald W. King as part of the conferences held at the Given Instituteof Pathobiology, University of Colorado, Aspen. It was felt that thiswas an important research area on which to hold an initial smallworkshop that would bring together most of the leading investigatorsin the field, at a time when several laboratories were attempting toestablish criteria for in vitro neoplastic cell transformationfollowing chemical treatment.

The workshop, chaired by Drs. Saffiotti and Weinstein, took placefrom July 27 to August 1, 1975, and it included 33 participants. Itwas supported by the National Cancer Institute and consisted oflectures, laboratory demonstrations, and laboratory work activelyinvolving all participants. The workshop was primarily devoted toreviewing laboratory methods, transformation criteria, and markers.The unique environment and intellectual atmosphere of the GivenInstitute at Aspen were particularly stimulating and the workshopsucceeded in fostering friendly, uninhibited, and constructivediscussion and cooperation among investigators from differentlaboratories. The participants found it a unique opportunity toreview each other's experimental material and to resolve controversialquestions of interpretation directly on site in the laboratory.

The participants in the 1975 workshop expressed their desire tomeet again in 1976 for a conference that would allow the findings ofthe leading research groups to be demonstrated to a larger audience.

On this basis, a "Seminar and Workshop on In VitroCarcinogenesis" was planned and organized by Drs. Umberto Saffiottiand Donald W. King with advice from several participants, and it washeld at the Given Institute of Pathobiology in Aspen from July 18 to23, 1976. Drs. U. Saffiotti and D. W. King served as chairmen of theconference and Dr. Paul 0. P. Ts'o as chairman of the laboratoryworkshop. This seminar was part of the series held at the GivenInstitute entitled "Advances in Cancer Biology" and supported by agrant (No. 5R13CA15961) from the National Cancer Institute.

The present publication is based on the presentations made atthis conference. The members of the seminar's faculty agreed that itwould not be desirable to publish the detailed papers which werepresented orally, since many involved discussion of previouslypublished results. Several good reviews were also recently publishedby some of the participants. A need was recognized, however, tocollect in a single publication the extensive literature that hasdeveloped in the area of neoplastic transformation of cells in cultureby chemical and physical agents. The editors suggested and thefaculty agreed that this publication be devoted to an extensivebibliography on the subject, with brief papers serving as a guide tothe literature on different topics. Selected papers give moreextensive reports of previously unpublished new advances. Inaddition, a section is devoted to the publication of detailedlaboratory procedures which are not available in the currentliterature.

The editors have arranged the sequences of papers around maintopics and edited their format and bibliography for consistency. Theywish to express their appreciation to the authors for theircooperation; to Dr. D. W. King, Director of the Given Institute ofPathobiology, for his sponsorship of the Conference; and to both Dr.D. W. King and Dr. Cecilia Fenoglio, Department of Pathology, ColumbiaUniversity, for their editorial advice.

The National Cancer Institute Carcinogenesis Technical ReportSeries, in which this volume appears, was established early in 1976,at the initiative of Drs. U. Saffiotti and N. P. Page, thenrespectively responsible for the NCI Carcinogenesis Program and forits Carcinogen Bioassays and Program Resources Branch, as a means topublish documentation methods, procedures, and findings in the fieldof Carcinogenesis bioassays and research, particularly when formatlimitations make it difficult to report detailed data through theconventional scientific journals.

It is hoped that this volume will be a useful contribution to therapidly developing field of Carcinogenesis studies by in vitro culturemethods.

Of the participants in the Symposium, four were unable to contributedirectly to this volume: J.A. DiPaolo, M.W. Lieberman, F.H.Ruddle, and I.E. Weinstein.The Editors

Report Date: 1978

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