National Toxicology Program

National Toxicology Program
https://ntp.niehs.nih.gov/go/9837

Target Organs and Levels of Evidence TR-528

Carcinogenesis Studies of 2,2-bis(Bromomethyl)-1,3-Propanediol, Nitromethane, and 1,2,3-Trichloropropane (CASRNs 3296-90-0, 75-52-5, and 96-18-4) in Guppies (Poecilia reticulata) and Medaka (Oryzias latipes) (Waterborne Studies)

Chemical (Study Title)
CASRN
Peer Review Date Primary Uses Route/Exposure Levels Study Laboratory
Fish Project 1 (Nitromethane)
75-52-5
02/18/2004 BMP-used as a fire retardant in unsaturated polyester resins, in molded products, & in rigid polyurethane foam. Nitromethane-used as a rocket & engine fuel; a synthesis intermediate for agricultural fumigants, biocides, & other products; a solvent; & an explosive. TCP-used as a paint & varnish remover, solvent, & degreasing agent, & a crosslinking agent in the synthesis of polysulfides & HFP. Aquatic
Medaka: 9.4, 21, OR 46.6 MG/L 160 FISH/DOSE/SPECIES
Gulf Coast Research Laboratory

Levels of Evidence

Male Fish: Negative
Female Fish: Negative


Chemical (Study Title)
CASRN
Peer Review Date Primary Uses Route/Exposure Levels Study Laboratory
Fish Project 1 (Nitromethane)
75-52-5
02/18/2004 BMP-used as a fire retardant in unsaturated polyester resins, in molded products, & in rigid polyurethane foam. Nitromethane-used as a rocket & engine fuel; a synthesis intermediate for agricultural fumigants, biocides, & other products; a solvent; & an explosive. TCP-used as a paint & varnish remover, solvent, & degreasing agent, & a crosslinking agent in the synthesis of polysulfides & HFP. Aquatic
Guppy: 10.8, 30.9, OR 88.4 MG/L 160 FISH/DOSE/SPECIES
Gulf Coast Research Laboratory

Levels of Evidence

Male Fish: Inadequate Study
Female Fish: Negative


Chemical (Study Title)
CASRN
Peer Review Date Primary Uses Route/Exposure Levels Study Laboratory
Fish project 1 (2,2-bis(Bromomethyl)-1,3-propanediol)
3296-90-0
02/18/2004 BMP-used as a fire retardant in unsaturated polyester resins, in molded products, & in rigid polyurethane foam. Nitromethane-used as a rocket & engine fuel; a synthesis intermediate for agricultural fumigants, biocides, & other products; a solvent; & an explosive. TCP-used as a paint & varnish remover, solvent, & degreasing agent, & a crosslinking agent in the synthesis of polysulfides & HFP. Aquatic
Guppy: 160 FISH/DOSE/SPECIES
Gulf Coast Research Laboratory

Levels of Evidence

Male Fish: Positive
Type Organ/Tissue (Lesion)
Neoplastic Lesions
  • Liver (Core Study): ADENOMA 3/61 4/50 4/41 8/38 ADENOMA OR CARCINOMA 4/61 6/50 6/41 10/38
  • Liver (Stop-Exposure Study): ADENOMA 0/28 3/22 3/21 6/24 ADENOMA OR CARCINOMA 0/28 3/22 3/21 9/24
Female Fish: Inadequate Study


Chemical (Study Title)
CASRN
Peer Review Date Primary Uses Route/Exposure Levels Study Laboratory
Fish project 1 (2,2-bis(Bromomethyl)-1,3-propanediol)
3296-90-0
02/18/2004 BMP-used as a fire retardant in unsaturated polyester resins, in molded products, & in rigid polyurethane foam. Nitromethane-used as a rocket & engine fuel; a synthesis intermediate for agricultural fumigants, biocides, & other products; a solvent; & an explosive. TCP-used as a paint & varnish remover, solvent, & degreasing agent, & a crosslinking agent in the synthesis of polysulfides & HFP. Aquatic
Medaka: 0, 24, 60, 150 MG/L 160 FISH/DOSE/SPECIES
Gulf Coast Research Laboratory

Levels of Evidence

Male Fish: Positive
Type Organ/Tissue (Lesion)
Neoplastic Lesions
  • Liver (Core Study): ADENOMA 1/47 0/59 0/56 6/59 ADENOMA OR CARCINOMA 1/47 0/59 0/56 8/59
  • Liver (Stop-Exposoure Study): ADENOMA 1/27 0/21 1/31 3/29 ADENOMA OR CARCINOMA 1/27 0/21 1/31 4/29
Female Fish: Negative


Chemical (Study Title)
CASRN
Peer Review Date Primary Uses Route/Exposure Levels Study Laboratory
Fish project 1 (1,2,3-Trichloropropane)
96-18-4
02/18/2004 BMP-used as a fire retardant in unsaturated polyester resins, in molded products, & in rigid polyurethane foam. Nitromethane-used as a rocket & engine fuel; a synthesis intermediate for agricultural fumigants, biocides, & other products; a solvent; & an explosive. TCP-used as a paint & varnish remover, solvent, & degreasing agent, & a crosslinking agent in the synthesis of polysulfides & HFP. Aquatic
Guppy: 0, 4.5, 9, OR 18 MG/L (2 REPLICATES PER TREATMENT GROUP) 170 FISH/EACH REPLICATE AQUARIUM
Gulf Coast Research Laboratory

Levels of Evidence

Male Fish: Positive
Type Organ/Tissue (Lesion)
Neoplastic Lesions
  • Liver (Core Study): CHOLANGIOCARCINOMA 0/61 0/47 0/67 3/27 ADENOMA 3/61 8/47 17/67 9/27 CARCINOMA 0/61 1/47 0/67 7/27 ADENOMA OR CARCINOMA COMBINED 3/61 9/47 17/67 15/27
  • Liver (Stop-Exposure Study): CHOLANGIOCARCINOMA 0/32 0/19 0/26 1/19 ADENOMA 3/32 4/19 5/26 7/19 CARCINOMA 0/32 0/19 1/26 4/19 ADENOMA OR CARCINOMA COMBINED 3/32 4/19 5/26 10/19
Female Fish: Positive
Type Organ/Tissue (Lesion)
Neoplastic Lesions
  • Liver (Core Study): CHOLANGIOMA 0/64 3/64 4/47 2/33 CHOLANGIOCARCINOMA 0/64 0/64 0/47 6/33 ADENOMA OR CARCINOMA 5/64 4/64 4/47 8/33
  • Liver (Stop-Exposure Study): CHOLANGIOMA 2/31 2/31 0/31 2/27 CHOLANGIOCARCINOMA 0/31 0/31 0/31 5/27 ADENOMA OR CARCINOMA 2/31 1/31 2/31 3/27


Chemical (Study Title)
CASRN
Peer Review Date Primary Uses Route/Exposure Levels Study Laboratory
Fish project 1 (1,2,3-Trichloropropane)
96-18-4
02/18/2004 BMP-used as a fire retardant in unsaturated polyester resins, in molded products, & in rigid polyurethane foam. Nitromethane-used as a rocket & engine fuel; a synthesis intermediate for agricultural fumigants, biocides, & other products; a solvent; & an explosive. TCP-used as a paint & varnish remover, solvent, & degreasing agent, & a crosslinking agent in the synthesis of polysulfides & HFP. Aquatic
Medaka: 0, 4.5, 9, OR 18 MG/L (2 REPLICATES PER TREATMENT GROUP) 170 FISH/EACH REPLICATE AQUARIUM
Gulf Coast Research Laboratory

Levels of Evidence

Male Fish: Positive
Type Organ/Tissue (Lesion)
Neoplastic Lesions
  • Liver (Core Study): CHOLANGIOMA 0/95 6/94 2/65 1/78 CHOLANGIOCARCINOMA 0/95 32/94 43/65 45/78 ADENOMA OR CARCINOMA 0/95 2/94 1/65 6/78 HEPATOCHOLANGIOCARCINOMA 0/95 0/94 2/65 6/78
  • Liver (Stop-Exposure Study): CHOLANGIOMA 0/42 0/47 3/33 1/46 CHOLANGIOCARCINOMA 0/42 19/47 16/33 26/46 ADENOMA 0/42 1/47 0/33 0/46 ADENOMA OR CARCINOMA 0/42 2/47 0/33 1/46
  • Gallbladder (Core Study): ADENOMA, PAPILLARY 0/91 0/83 4/61 9/72
  • Gallbladder (Stop-Exposure Study): ADENOMA, PAPILLARY 0/41 0/42 3/30 4/46
Female Fish: Positive
Type Organ/Tissue (Lesion)
Neoplastic Lesions
  • Liver (Core Study): CHOLANGIOCARCINOMA 0/95 29/93 53/94 41/67 ADENOMA 1/95 1/93 5/94 8/67 CARCINOMA 1/95 1/93 2/94 5/67 ADENOMA OR CARCINOMA 2/95 2/93 7/94 13/67 HEPATOCHOLANGIOCARCINOMA 0/95 0/93 4/94 7/67
  • Liver (Stop Exposure Study): CHOLANGIOCARCINOMA 1/57 15/44 31/49 21/31 ADENOMA 4/57 2/44 2/49 1/31 CARCINOMA 0/57 2/44 1/49 4/31 ADENOMA OR CARCINOMA 4/57 4/44 2/49 5/31 HEPATOCHOLANGIOCARCINOMA 0/57 0/44 0/49 1/31
  • Gallbladder (Core Study): ADENOMA, PAPILLARY 0/85 1/80 5/88 6/65
  • Gallbladder (Stop-Exposure Study): ADENOMA, PAPILLARY 0/56 1/40 1/45 1/30


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