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Testing Status of Rotenone 10420-W

CASRN: 83-79-4

Formula: C23-H22-O6

Synonyms/Common Names

  • (2R-(2-alpha,6Aalpha,12Aalpha))-1,2,12,12A-tetrahydro-8,9-dimethoxy-2-(1-methylethenyl)-(1)benzopyrano(3,4-B)furo(2,3-H)(1)benzopyran-6(6aH)-one (9CI)

Short-Term Toxicity

  • 2 weeks (Dosed-Feed)  (C55210B)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 13 weeks (Dosed-Feed)  (C55210B)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0, 75, 150, 300, 600, OR 1200 PPM M: 0, 600, 1900, 5000, 16000, OR 50000 PPM.

Long-Term Carcinogenicity

  • 88 weeks (Intraperitoneal Injection)  (C55210A)  Completed 
    •   (Peer Review Approval NA) Citation: Tumorogenic Potential of Rotenone and its Specificity for Mammary Tissue, published by National Center for Toxicological Research (NCTR Experiment No. 216). Call NCTR (501-543-7115) for availability of document.
    • Rats: F344/N
    • Dose: 986 & 1972 UG/KG - 5 DAYS/WK FOR 42 INJ. DAYS/72 PER GROUP.
  • 2 years (Dosed-Feed)  (C55210B)  Completed 
    • TR-320 (NIH Number: 88-2576)  (Peer Review Approval 08/19/1986A )
      Toxicology and Carcinogenesis Studies of Rotenone (CASRN 83-79-4) in F344/N Rats and B6C3F1 Mice (Feed Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0, 38, OR 75 PPM M: 0, 600, OR 1200 PPM; 50 PER GROUP.
    • Carcinogenesis Results
      • Male Rats Equivocal Evidence 
      • Female Rats No Evidence 
      • Male Mice No Evidence 
      • Female Mice No Evidence 

Special Studies

  • C. elegans  (W10420)  Completed 
    • C. elegans [CE]
    • Dose: 100, 75, 50 uM.
  • Cell Proliferation (Dosed-Feed)  (S0590)  Completed 
    • Citation: Cunningham ML, Soliman MS, Badr MZ, Matthews HB. Rotenone, an anticarcinogen, inhibits cellular proliferation but not peroxisome proliferation in mouse liver. Cancer Lett. 1995 Aug 16;95(1-2):93-7.Pubmed Abstract
    • Mice [M]: B6C3F1
    • Dose: (dosed-feed) 600 ppm [M].

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (195015)  Completed 
    • Citation: Anderson BE, Zeiger E, Shelby MD, Resnick MA, Gulati DK, Ivett JL, Loveday KS. Chromosome aberration and sister chromatid exchange test results with 42 chemicals. Environ Mol Mutagen. 1990;16 Suppl 18:55-137. doi: 10.1002/em.2850160505
    • Sister Chromatid Exchange Not a Valid Test 
    • Chromosome Aberrations Negative 
  • In Vitro Cytogenetics (CA/SCE)  (743095)  Completed 
    • Citation: Anderson BE, Zeiger E, Shelby MD, Resnick MA, Gulati DK, Ivett JL, Loveday KS. Chromosome aberration and sister chromatid exchange test results with 42 chemicals. Environ Mol Mutagen. 1990;16 Suppl 18:55-137. doi: 10.1002/em.2850160505
    • Sister Chromatid Exchange Weakly Positive 
    • Chromosome Aberrations Not Tested 
  • Mouse Lymphoma  (542524)  Completed 
    • Citation: McGregor DB, Brown A, Cattanach P, Edwards I, McBride D, Riach C, Caspary WJ. Responses of the L5178Y tk+/tk- mouse lymphoma cell forward mutation assay: III. 72 coded chemicals. Environ Mol Mutagen. 1988;12(1):85-154. doi: 10.1002/em.2860120111. Erratum in: Environ Mol Mutagen 1988;12(3):345
    • Citation: Myhr B, McGregor D, Bowers L, Riach C, Brown AG, Edwards I, McBride D, Martin R, Caspary WJ. L5178Y mouse lymphoma cell mutation assay results with 41 compounds. Environ Mol Mutagen. 1990;16 Suppl 18:138-67. doi: 10.1002/em.2850160506
    • Mice
    •  Positive 
  • Salmonella  (635953)  Completed 
    • Citation: Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K, Speck W. Salmonella mutagenicity tests: III. Results from the testing of 255 chemicals. Environ Mutagen. 1987;9 Suppl 9:1-109.
    •  Negative