Skip to Main Navigation
Skip to Page Content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Testing Status of Isobutyl nitrite 10869-J

 

CASRN: 542-56-3

Formula: C4-H9-N-O2

Synonyms/Common Names

  • Isobutyl nitrite (AIDS)
  • Nitrous acid, 2-methylpropyl ester (9CI)

Short-Term Toxicity

  • 2 weeks (Inhalation)  (C61052B)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 13 weeks (Inhalation)  (C61052B)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 10, 25, 75, 150, OR 300 PPM; 6 HR/DAY, 5 DAY/WEEK, 10/GROUP.

Long-Term Carcinogenicity

  • 2 years (Inhalation)  (C61052B)  Completed 
    • TR-448 (NIH Number: 96-3364)  (Peer Review Approval 11/29/1994A )
      Toxicology and Carcinogenesis Studies of Isobutyl Nitrite in F344/N Rats and B6C3F1 Mice (Inhalation Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Clear Evidence 
      • Female Rats Clear Evidence 
      • Male Mice Some Evidence 
      • Female Mice Some Evidence 
    • Dose: R&M: 0, 37, 75, OR 150 PPM.

Genetic Toxicology

  • Drosophila  (399025)  Completed 
    • Citation: Woodruff, R.C., Mason, J.M., Valencia, R., and Zimmering, S. Chemical mutagenesis testing in Drosophila: V. Results of 53 coded compounds tested for the National Toxicology Program. Environ. Mutagen. Vol. 7 (1985) 677-702
    • Reciprocal Translocation/Sex-Linked Recessive Lethal Negative 
  • In Vitro Cytogenetics (CA/SCE)  (016060)  Completed 
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Positive 
  • In Vitro Cytogenetics (CA/SCE)  (841266)  Completed 
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Positive 
  • Micronucleus  (A87798)  Completed 
    • Citation: Witt, K.L., Knapton, A., Wehr, C.M., Hook, G.J., Mirsalis, J., Shelby, M.D., and MacGregor, J.T. Micronucleated Erythrocyte Frequency in Peripheral Blood of B6C3F1 Mice from Short-Term, Prechronic, and Chronic Studies of the NTP Carcinogenesis Bioassay Program. Environ. Molec. Mutagen. Vol. 36 (2000) 163-194
    • Mice: B6C3F1
    • Male Positive 
    • Female Positive 
  • Salmonella  (234350)  Completed 
    • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
    •  Positive 
  • Salmonella  (507924)  Completed 
    •  Positive 
  • Salmonella  (983397)  Completed 
    •  Positive