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Testing Status of Isoprene M200028

CASRN: 78-79-5

Formula: C5-H8

Synonyms/Common Names

  • 1,3-Butadiene, 2-methyl- (9CI)

Short-Term Toxicity

  • 2 weeks (Inhalation)  (C61825)  Completed 
    • TOX-31 (NIH Number: 95-3354)
      Toxicity Studies of Isoprene (CASRN 78-79-5) Administered by Inhalation to F344/N Rats and B6C3F1 Mice
    • Rats: F344/N; Mice: B6C3F1
  • 13 weeks (Inhalation)  (C61825)  Completed 
    • TOX-31 (NIH Number: 95-3354)  (Tox Peer Review 11/17/1993A )
      Toxicity Studies of Isoprene (CASRN 78-79-5) Administered by Inhalation to F344/N Rats and B6C3F1 Mice
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0, 70, 220, 700, 2200, 7000 PPM.
  • 26 weeks (Inhalation)  (C61825C)  Completed 
    • TOX-31 (NIH Number: 95-3354)  (Tox Peer Review 11/17/1993A )
      Toxicity Studies of Isoprene (CASRN 78-79-5) Administered by Inhalation to F344/N Rats and B6C3F1 Mice
    • Rats: F344/N; Mice: B6C3F1
    • Dose: MALES ONLY; R&M: 0, 70, 220, 700, 2200, 7000 PPM.

Long-Term Carcinogenicity

  • 2 years (Inhalation)  (C61825B)  Completed 
    • TR-486 (NIH Number: 99-3976)  (Peer Review Approval 12/10/1997A )
      Toxicology and Carcinogenesis Studies of Isoprene (CASRN 78-79-5) in F344/N Rats (Inhalation Studies)
    • Rats: F344/N
    • Dose: R: 0, 220, 700, OR 7000 PPM; 50/SEX/GROUP.
    • Carcinogenesis Results
      • Male Rats Clear Evidence 
      • Female Rats Some Evidence 

Special Studies

  • Absorption Distribution Metabolism Excretion (Inhalation)  (S0227)  Completed 
    • Citation: Bond JA, Bechtold WE, Birnbaum LS, Dahl AR, Medinsky MA, Sun JD, Henderson RF. Disposition of inhaled isoprene in B6C3F1 mice. Toxicol Appl Pharmacol. 1991 Mar 1;107(3):494-503. Pubmed Abstract
    • Citation: Dahl AR, Birnbaum LS, Bond JA, Gervasi PG, Henderson RF. The fate of isoprene inhaled by rats: comparison to butadiene. Toxicol Appl Pharmacol. 1987 Jun 30;89(2):237-48.Pubmed Abstract
    • Male Rats [R]: F344/N
  • Absorption Distribution Metabolism Excretion (Intraperitoneal Injection)  (S0460)  Completed 
    • Citation: Sun JD, Dahl AR, Bond JA, Birnbaum LS, Henderson RF. Characterization of hemoglobin adduct formation in mice and rats after administration of [14C]butadiene or [14C]isoprene. Toxicol Appl Pharmacol. 1989 Aug;100(1):86-95.Pubmed Abstract
    • Male Rats [R]: Sprague Dawley; Male Mice [M]: B6C3F1
    • Dose: (intraperitoneal injection) 0.3, 3.0, 300, 1000 or 3000 umol [14C]Isoprene [R,M].
  • Metabolism (Intraperitoneal Injection)  (S0338)  Completed 
    • Male Rats [R]: F344/N; Male Mice [M]: B6C3F1
    • Dose: (intraperitoneal injection) 66.6 mg/kg, pilot 1; 66.2 mg/kg, pilot 2; 66.4 mg/kg, study A [R]. (intraperitoneal injection) 33.2 mg/kg, pilot 1; 68.4 mg/kg, study CM [M].
  • Modeling  (S0648)  Completed 
    • []

Genetic Toxicology

  • Chromosome Aberrations  (A63977)  Completed 
    • Mice
    •  Negative (Nonstandard Protocol) 
  • In Vitro Cytogenetics (CA/SCE)  (032841)  Completed 
    • Sister Chromatid Exchange Negative 
    • Chromosome Aberrations Negative 
  • Micronucleus  (A28566)  Completed 
    • Citation: Witt KL, Knapton A, Wehr CM, Hook GJ, Mirsalis J, Shelby MD, MacGregor JT. Micronucleated erythrocyte frequency in peripheral blood of B6C3F(1) mice from short-term, prechronic, and chronic studies of the NTP carcinogenesis bioassay program. Environ Mol Mutagen. 2000;36(3):163-94
    • Mice: B6C3F1
    • Male Positive 
    • Female Positive 
  • Micronucleus  (A63977)  Completed 
    • Mice: B6C3F1
    • Male Positive 
  • Salmonella  (453451)  Completed 
    • Citation: Mortelmans K, Haworth S, Lawlor T, Speck W, Tainer B, Zeiger E. Salmonella mutagenicity tests: II. Results from the testing of 270 chemicals. Environ Mutagen. 1986;8 Suppl 7:1-119
    •  Negative 
  • Sister Chromatid Exchanges  (A63977)  Completed 
    • Mice
    • Sister Chromatid  Positive (Nonstandard Protocol) 
  • Sister Chromatid Exchanges  (C78795)  Completed 
    • Citation: Tice RR, Boucher R, Luke CA, Paquette DE, Melnick RL, Shelby MD. Chloroprene and isoprene: cytogenetic studies in mice. Mutagenesis. 1988 Mar;3(2):141-6. doi: 10.1093/mutage/3.2.141
    • Mice
    • Sister Chromatid  Positive 

Organ Systems Toxicity

  • 6 to 17 GD for mice Conventional Teratology (Inhalation)  (TER88045)  Completed 
    • Female Mice: Swiss
    • Dose: 0, 280, 1400, 7000 ppm.
    • Mice Maternal Positive 
    • Mice Fetal Positive 
  • 6 to 19 GD Conventional Teratology (Inhalation)  (TER88142)  Completed 
    • Rats: Sprague Dawley
    • Dose: 0, 280, 1400, 7000 ppm.
    • Rats Maternal Negative 
    • Rats Fetal Negative