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Testing Status of alpha/beta Thujone mixture M990011

CASRN: 76231-76-0

Formula: C10-H16-O

Synonyms/Common Names

  • Mixture of alpha & beta Thujone

Secondary:

  • 546-80-5 - alpha-Thujone
  • 471-15-8 - beta-Thujone

Short-Term Toxicity

  • 5 days (Gavage)  (C99011)  Completed 
    • 5-Day Transcriptomic Dose-Response Studies
    • Citation: Gwinn WM, Auerbach SS, Parham F, Stout MD, Waidyanatha S, Mutlu E, Collins B, Paules RS, Merrick BA, Ferguson S, Ramaiahgari S, Bucher JR, Sparrow B, Toy H, Gorospe J, Machesky N, Shah RR, Balik-Meisner MR, Mav D, Phadke DP, Roberts G, DeVito MJ. Evaluation of 5-day In Vivo Rat Liver and Kidney With High-throughput Transcriptomics for Estimating Benchmark Doses of Apical Outcomes. Toxicol Sci. 2020 Aug 1;176(2):343-354. doi: https://doi.org/10.1093/toxsci/kfaa081.Pubmed Abstract
    • Rats: Harlan Sprague-Dawley
    • Dose: Male rats: 0, 1.5, 3, 6.25, 12.5, 25, 50, 100, or 200 mg/kg; N=4.
  • 2 weeks (Gavage)  (C99037)  Completed 
    • Rats: F344/N; Mice: B6C3F1/N
    • Dose: 0, 1, 3, 10, 30, OR 100 MG/KG/DAY.
  • 13 weeks (Gavage)  (C99037B)  Completed 
    • Rats: F344/N; Mice: B6C3F1/N
    • Dose: R: 0, 12.5, 25, 50, 75, OR 100 MG/KG M: 0, 6.25, 12.5, 25, 50, OR 75 MG/KG; 10/SEX/SPECIES, 5X/WEEK.

Long-Term Carcinogenicity

  • 2 years (Gavage)  (C99037B)  Completed 
    • TR-570 (NIH Number: 11-5912)  (Peer Review Approval 01/26/2011A )
      Toxicology and Carcinogenesis Studies of alpha,beta-Thujone (CASRN 76231-76-0) in F344/N Rats and B6C3F1 Mice (Gavage Studies)
    • Rats: F344/N; Mice: B6C3F1/N
    • Dose: R: 0, 12.5, 25, OR 50 MG/KG M: 0, 3, 6, 12, OR 25 MG/KG 50/SEX/SPECIES.
    • Carcinogenesis Results
      • Male Rats Some Evidence 
      • Female Rats No Evidence 
      • Male Mice No Evidence 
      • Female Mice No Evidence 

Special Studies

  • Toxicokinetic Study (Gavage; Intravenous; Gavage; Intravenous)  (K06898)  Completed 
    • Citation: Waidyanatha S, Johnson JD, Hong SP, Robinson VG, Gibbs S, Graves SW, Hooth MJ, Smith CS. Toxicokinetics of α-thujone following intravenous and gavage administration of α-thujone or α- and β-thujone mixture in male and female F344/N rats and B6C3F1 mice. Toxicol Appl Pharmacol. 2013 Sep 271(2):216-28. doi: 10.1016/j.taap.2013.05.001. Epub 2013 May 10.Pubmed Abstract
    • Male and Female Rats [R]: Fischer 344; Male and Female Mice [M]: B6C3F1
    • Dose: (gavage) 25, 50 mg/kg [R]. (intravenous) 1.6, 3.0 mg/kg [R]. (gavage) 40, 80 mg/kg [M]. (intravenous) 3.2, 6.0 mg/kg [M].

Genetic Toxicology

  • Micronucleus  (A26162)  Completed 
    • Mice: B6C3F1
    • Male Negative 
    • Female Positive 
  • Salmonella  (A36495)  Completed 
    •  Negative 

Toxicogenomics

  • 5 days Transcriptome Sequencing (Gavage)  (T99011)  Completed 
    • 5-Day Transcriptomic Dose-Response Studies
    • Citation: Gwinn WM, Auerbach SS, Parham F, Stout MD, Waidyanatha S, Mutlu E, Collins B, Paules RS, Merrick BA, Ferguson S, Ramaiahgari S, Bucher JR, Sparrow B, Toy H, Gorospe J, Machesky N, Shah RR, Balik-Meisner MR, Mav D, Phadke DP, Roberts G, DeVito MJ. Evaluation of 5-day In Vivo Rat Liver and Kidney With High-throughput Transcriptomics for Estimating Benchmark Doses of Apical Outcomes. Toxicol Sci. 2020 Aug 1;176(2):343-354. doi: https://doi.org/10.1093/toxsci/kfaa081.Pubmed Abstract
    • Rats: Harlan Sprague-Dawley