National Toxicology Program

National Toxicology Program

Testing Status of Toluene - 10009-V


CASRN: 108-88-3


Formula: C7-H8

Synonyms/Common Names

  • Methylbenzene (9CI)

Known Uses

Chemical intermediate; solvent; denaturant. Used to back-blend gasoline. Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 13 weeks (Inhalation)  (C07272B)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: GAV:(C07272A) 0,312, 625,1250,2500,5000 MG/KG; INHAL:0,100, 625,1250,2500,3000 PPM/10 PER GROUP.
  • 13-Week (Gavage)  (C07272C)  Completed 
    • Rats: F344/N; Mice: B6C3F1

Long-Term Carcinogenicity

  • 15 months & 2 years (Inhalation)  (C07272B)  Report Complete 
    • TR-371 (NIH Number: 90-2826)  (Peer Review Approval 03/13/1989A )
      Toxicology and Carcinogenesis Studies of Toluene (CASRN108-88-3) in F344/N Rats and B6C3F1 Mice (Inhalation Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats No Evidence 
      • Female Rats No Evidence 
      • Male Mice No Evidence 
      • Female Mice No Evidence 
    • Dose: R: 0,600,1200, M: 0,120,600,1200 PPM/60 PER GROUP.

Special Studies

  • Absorption Disposition Metabolism Elimination (Gavage)  (S0615)  Completed 
    • Male Rats: F344/N; Male Mice: B6C3F1
  • Metabolism  (S0714)  Completed 
    • Citation: Chapman DE, Moore TJ, Michener SR, Powis G. Metabolism and covalent binding of [14C]toluene by human and rat liver microsomal fractions and liver slices. Drug Metab Dispos. 1990 Nov-Dec;18(6):929-36.Pubmed Abstract
    • Male Rats: F344/N; Human (Cell Lines)

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (638654)  Completed 
    • Sister Chromatid Exchange Negative 
    • Chromosome Aberrations Negative 
  • Mouse Lymphoma  (434629)  Completed 
    • Citation: McGregor, D., Brown, A., Cattanach, P., Edwards, I., McBride, D., Riach, C., and Caspary, W. Responses of the L5178Y tk+/tk- mouse lymphoma cell forward mutation assay: III. 72 coded chemicals. Environ. Molec. Mutagen. Vol. 12 (1988) 85-154
    • Mice
    •  Equivocal 
  • Salmonella  (132615)  Completed 
    • Citation: Haworth, S., Lawlor, T., Mortelmans, K., Speck, W., and Zeiger, E. Salmonella mutagenicity test results for 250 chemicals Environ. Mutagen. Vol. 5 (Suppl 1) (1983) 3-142
    •  Negative 

Organ Systems Toxicity

  • Neurotoxicology Assessment (Inhalation)  (NTA94013)  Completed 
    • Citation: Animal Neurobehavioral Test Battery in NTP Assessment In: Advances in Neurobehavioral Toxicology: Applications in Environmental & Occupational Health (Barry L. Johnson, ED.), Lewis Publishers, Inc. pp. 403-418 (1990).
    • Male Rats: F344/N
  • 8 consecutive days (MTD PHASE), 7 to 14 GD (reproductive phase) Short-Term in vivo Repro. Tox. (Gavage)  (T0063A)  Completed 
    • Citation: Determination of the Reproductive Effects in Mice of Nine Selected Chemicals
    • Female Mice: Swiss
    • Dose: 2350 MG/KG BD WT.
  • 6 to 13 GD Short-Term in vivo Repro. Tox. (Gavage)  (T0063B)  Completed 
    • Female Mice: Swiss
    • Dose: 3000 MG/KG/D.
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.