National Toxicology Program

National Toxicology Program
http://ntp.niehs.nih.gov/go/ts-10030-h

Testing Status of Methylene chloride - 10030-H

 

CASRN: 75-09-2

Formula: C-H2-Cl2

Synonyms/Common Names

  • Dichloromethane
  • Dichloromethane

Known Uses

Solvent in paint removers, degreasers, aerosol propellants, extraction solvent in food processing; pharmaceutical aid, component of fire extinguishing compound (HSDB 1990). Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 13-Week (Inhalation)  (C50102B)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: 0, 525, 8400 PPM/10 PER GROUP.

Long-Term Carcinogenicity

  • 2 years (Inhalation)  (C50102B)  Report Complete 
    • TR-306 (NIH Number: 86-2562)  (Peer Review Approval 03/29/1985A )
      Toxicology and Carcinogenesis Studies of Dichloromethane (Methylene Chloride) (CASRN 75-09-2) in F344/N Rats and B6C3F1 Mice (Inhalation Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Some Evidence 
      • Female Rats Clear Evidence 
      • Male Mice Clear Evidence 
      • Female Mice Clear Evidence 
    • Dose: R: 0, 1000, 2000, 4000, M: 0, 2000, 4000 PPM/50 PER GROUP.

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (676332)  Completed 
    • Citation: Anderson, B.E., Zeiger, E., Shelby, M.D., Resnick, M.A., Gulati, D.K., Ivett, J.L., and Loveday, K.S. Chromosome aberration and sister chromatid exchange test results with 42 chemicals. Environ. Molec. Mutagen. Vol. 16 (Suppl 18) (1990) 55-137
    • Sister Chromatid Exchange Negative 
    • Chromosome Aberrations Negative 
  • Mouse Lymphoma  (502376)  Completed 
    • Citation: Myhr B.C., McGregor, D., Bowers, L., Riach, C., Brown, A., Edwards, I., McBride, D., Martin, R., and Caspary, W.J. L5178Y mouse lymphoma cell mutation assay results with 41 compounds. Environ. Molec. Mutagen. Vol. 16 (Suppl 18) (1990) 138-167
    • Mice
    •  Equivocal 
  • Salmonella  (070295)  Completed 
    • Citation: Zeiger, E. Mutagenicity of 42 chemicals in Salmonella Environ. Molec. Mutagen. Vol. 16(Suppl 18) (1990) 32-54
    •  Positive 
  • Salmonella  (413864)  Completed 
    • Citation: Zeiger, E. Mutagenicity of 42 chemicals in Salmonella Environ. Molec. Mutagen. Vol. 16(Suppl 18) (1990) 32-54
    •  Negative 
  • Salmonella  (A34199)  Completed 
    •  Positive 
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.