Skip to Main Navigation
Skip to Page Content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.


The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Testing Status of n-Hexane 10189-N


CASRN: 110-54-3

Formula: C6-H14

Synonyms/Common Names

  • Hexane (8CI)(9CI)

Known Uses

Determination of refractive index of minerals, calibrations, reaction medium, solvent, alcohol denaturant, pharmaceuticals, paint diluent. Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 13 weeks (Inhalation)  (C60571)  Completed 
    • Mice: B6C3F1
    • Dose: 0, 500, 1000, 4000, 10000PPM.

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (587411)  Completed 
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Negative 
  • Micronucleus  (A42410)  Completed 
    • Citation: Witt, K.L., Knapton, A., Wehr, C.M., Hook, G.J., Mirsalis, J., Shelby, M.D., and MacGregor, J.T. Micronucleated Erythrocyte Frequency in Peripheral Blood of B6C3F1 Mice from Short-Term, Prechronic, and Chronic Studies of the NTP Carcinogenesis Bioassay Program. Environ. Molec. Mutagen. Vol. 36 (2000) 163-194
    • Mice: B6C3F1
    • Male Negative 
    • Female Negative 
  • Salmonella  (701057)  Completed 
    • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
    •  Negative 
  • Sister Chromatid Exchanges  (699016)  Completed 
    • Mice
    • Sister Chromatid  Negative (Nonstandard Protocol) 

Organ Systems Toxicity

  • 5 consecutive days Dominant Lethal (Inhalation)  (T0185B)  Completed 
    • Citation: Inhalation Reproductive Toxicology Studies: Male Dominant Lethal Study of N-Hexane in Swiss (CD-1) Mice (August 1988)
    • Male Mice: Swiss
    • Dose: 200, 1000, 5000 PPM; 20 HR/DAY.
  • Neurotoxicology Assessment (Inhalation)  (NTA94015)  Completed 
    • Citation: Animal Neurobehavioral Test Battery in NTP Assessment In: Advances in Neurobehavioral Toxicology: Applications in Environmental & Occupational Health (Barry L. Johnson, ED.), Lewis Publishers, Inc. pp. 403-418 (1990).
    • Male and Female Rats/Mice: F344/N
  • 6 to 19 GD Conventional Teratology (Inhalation)  (TER90082)  Completed 
    • Citation: Inhalation Developmental Toxicology Studies: Teratology Study of N-Hexane in Rats (December 1987)
    • Female Rats: Sprague Dawley
    • Rats Maternal Positive 
    • Rats Fetal Positive 
    • Dose: 200, 1000, 5000 PPM.
  • 6 to 17 GD Conventional Teratology (Inhalation)  (TER90083)  Completed 
    • Citation: Inhalation Developmental Toxicology Studies: Teratology Study of n-Hexane in Mice
    • Female Mice: Swiss
    • Mice Maternal Positive 
    • Mice Fetal Positive 
    • Dose: 200, 1000, 5000 PPM.