National Toxicology Program

National Toxicology Program

Testing Status of o-Nitroanisole - 10211-G


CASRN: 91-23-6

Formula: C7-H7-N-O3

Synonyms/Common Names

  • 1-Methoxy-2-nitrobenzene (9CI)
  • Nitrophenyl methyl ether

Known Uses

Used as an intermediate for the preparation of o-anisidine and in the manufacture of azo dyes

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 14-Day (Dosed-Feed)  (C60388A)  Completed 
    • Rats: F344/N; Mice: B6C3F1
  • 13-Week (Dosed-Feed)  (C60388A)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0,200,18000, M: 0,60,6000 PPM/10 PER GROUP.

Long-Term Carcinogenicity

  • 2 years (Dosed-Feed)  (C60388B)  Report Complete 
    • TR-416 (NIH Number: 92-3147)  (Peer Review Approval 11/21/1991A )
      Toxicology and Carcinogenesis Studies of o-Nitroanisole (CASRN 91-23-6) in F344 Rats and B6C3F1 Mice (Feed Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Clear Evidence 
      • Female Rats Clear Evidence 
      • Male Mice Clear Evidence 
      • Female Mice Some Evidence 
    • Dose: (CORE STUDY) R: 0, 222, 666, 2000 PPM M: 0, 666, 2000, 6000 PPM (STOP STUDY) R: 0, 6000, 18000 PPM; 50/GROUP.

Special Studies

  • Absorption Disposition Metabolism Elimination (Gavage; Intravenous)  (S0028)  Completed 
    • Citation: Miller MJ, Sipes IG, Perry DF, Carter DE. Pharmacokinetics of o-nitroanisole in Fischer 344 rats. Drug Metab Dispos. 1985 Sep-Oct;13(5):527-31.Pubmed Abstract
    • Male Rats: F344/N
    • Dose: PO: 5,50 MG/KG IV: 25 MG/KG.

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (410303)  Completed 
    • Citation: Galloway, S., Armstrong, M., Reuben, C., Colman, S., Brown, B., Cannon, C., Bloom, A., Nakamura, F., Ahmed, M., Duk, S., Rimpo, J., Margolin, B., Resnick, M., Anderson, B., and Zeiger, E. Chromosome aberration and sister chromatid exchanges in Chinese hamster ovary cells: Evaluations of 108 chemicals. Environ. Molec. Mutagen. Vol. 10 (Suppl 10) (1987) 1-176
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Weakly Positive 
  • Mouse Lymphoma  (578918)  Completed 
    • Mice
    •  Positive 
  • Salmonella  (178197)  Completed 
    •  Positive 
  • Salmonella  (205865)  Completed 
    • Citation: Haworth, S., Lawlor, T., Mortelmans, K., Speck, W., and Zeiger, E. Salmonella mutagenicity test results for 250 chemicals Environ. Mutagen. Vol. 5 (Suppl 1) (1983) 3-142
    •  Positive 
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.