National Toxicology Program

National Toxicology Program
http://ntp.niehs.nih.gov/go/ts-10251-n

Testing Status of Citral - 10251-N

 

CASRN: 5392-40-5

Formula: C10-H16-O

Synonyms/Common Names

  • 3,7-Dimethyl-2,6-octadienal

Known Uses

Perfumery, flavoring agent, intermediate in vitamin A manufacture, main constituent of oil of lemon, orange. Associated with tobacco: reported either as a natural component of tobacco, pyrolysis product (in tobacco smoke), or additive for one or more types of tobacco products.

Chemical Properties

Toxicity Effects (HSDB)

Short-Term Toxicity

  • 2 weeks (Gavage)  (C56348B)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0, 0.625, 1.25, AND 2.5% BY WEIGHT.
  • 2 weeks (Microencapsulation in Feed)  (C56348C)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0, 0.625, 1.25, 2.5, 5.0, AND 10% BY WEIGHT/5 PER GROUP.
  • 13 weeks (Microencapsulation in Feed)  (C56348A)  Completed 
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0, 3900, 7800, 15600, OR 31300 PPM.

Long-Term Carcinogenicity

  • 2 years (Microencapsulation in Feed)  (C56348A)  Report Complete 
    • TR-505 (NIH Number: 03-4439)  (Peer Review Approval 05/03/2001A )
      Toxicology and Carcinogenesis Studies of Citral (Microencapsulated) (CASRN 5392-40-5) in F344/N Rats and B6C3F1 Mice (Feed Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats No Evidence 
      • Female Rats No Evidence 
      • Male Mice No Evidence 
      • Female Mice Equivocal Evidence 
    • Dose: R: 0 (UNTREATED), 0 (VEHICLE), 1000, 2000, OR 4000 PPM M: 0 (UNTREATED), 0 (VEHICLE), 500, 1000, OR 2000 PPM.

Special Studies

  • Absorption Disposition Metabolism Elimination (Gavage; Intravenous)  (S0087)  Completed 
    • Citation: Diliberto JJ, Usha G, Birnbaum LS. Disposition of citral in male Fischer rats. Drug Metab Dispos. 1988 Sep-Oct;16(5):721-7.Pubmed Abstract
    • Male Rats: F344/N
  • Metabolism (Gavage)  (S0002)  Completed 
    • Citation: Diliberto JJ, Srinivas P, Overstreet D, Usha G, Burka LT, Birnbaum LS. Metabolism of citral, an alpha,beta-unsaturated aldehyde, in male F344 rats. Drug Metab Dispos. 1990 Nov-Dec;18(6):866-75.Pubmed Abstract
    • Male Rats: F344/N
    • Dose: 5, 500 MG/KG.

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (881385)  Completed 
    • Sister Chromatid Exchange Positive 
    • Chromosome Aberrations Negative 
  • Micronucleus  (A66944)  Completed 
    • Mice: B6C3F1
    • Male Negative 
  • Micronucleus  (A84250)  Completed 
    • Mice: B6C3F1
    • Male Negative 
    • Female Negative 
  • Salmonella  (110259)  Completed 
    • Citation: Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K, Speck W. Salmonella mutagenicity tests: III. Results from the testing of 255 chemicals. Environ Mutagen. 1987;9 Suppl 9:1-109.
    •  Negative 
NTP is located at the National Institute of Environmental Health Sciences, part of the National Institutes of Health.